LOCUS       DAFCNJ010000001       180987 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000001 DAFCNJ010000000
VERSION     DAFCNJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 180987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 180987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..180987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.001"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(12..1535)
                     /locus_tag="LNE35_000001"
     CDS             complement(12..1535)
                     /locus_tag="LNE35_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBK6909196.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="LNE35_000002"
     CDS             1787..2968
                     /locus_tag="LNE35_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6909197.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="LNE35_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="LNE35_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBK6909198.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="LNE35_000004"
     CDS             complement(5053..7764)
                     /locus_tag="LNE35_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909199.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="LNE35_000005"
     CDS             complement(7774..8475)
                     /locus_tag="LNE35_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909200.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="LNE35_000006"
     CDS             8661..9260
                     /locus_tag="LNE35_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6909201.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="LNE35_000007"
     CDS             9341..10354
                     /locus_tag="LNE35_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK6909202.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="LNE35_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="LNE35_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBK6909203.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="LNE35_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="LNE35_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBK6909204.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="LNE35_000010"
     CDS             11802..13175
                     /locus_tag="LNE35_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6909205.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="LNE35_000011"
     CDS             13532..15676
                     /locus_tag="LNE35_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK6909206.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="LNE35_000012"
     CDS             complement(15728..16768)
                     /locus_tag="LNE35_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK6909207.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="LNE35_000013"
     CDS             complement(17045..18847)
                     /locus_tag="LNE35_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909208.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="LNE35_000014"
     CDS             complement(18861..19613)
                     /locus_tag="LNE35_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909209.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="LNE35_000015"
     CDS             complement(19622..19984)
                     /locus_tag="LNE35_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6909210.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="LNE35_000016"
     CDS             complement(20160..21032)
                     /locus_tag="LNE35_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK6909211.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="LNE35_000017"
     CDS             complement(21058..21570)
                     /locus_tag="LNE35_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBK6909212.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="LNE35_000018"
     CDS             complement(21708..22499)
                     /locus_tag="LNE35_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBK6909213.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="LNE35_000019"
     CDS             complement(22554..23162)
                     /locus_tag="LNE35_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBK6909214.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="LNE35_000020"
     CDS             complement(23155..23472)
                     /locus_tag="LNE35_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6909215.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="LNE35_000021"
     CDS             complement(23628..24356)
                     /locus_tag="LNE35_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBK6909216.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="LNE35_000022"
     CDS             24515..25396
                     /locus_tag="LNE35_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6909217.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="LNE35_000023"
     CDS             complement(25633..26676)
                     /locus_tag="LNE35_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6909218.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="LNE35_000024"
     CDS             complement(26679..27347)
                     /locus_tag="LNE35_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6909219.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="LNE35_000025"
     CDS             27489..29825
                     /locus_tag="LNE35_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6909220.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="LNE35_000026"
     CDS             complement(29940..30251)
                     /locus_tag="LNE35_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909221.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="LNE35_000027"
     CDS             30432..31907
                     /locus_tag="LNE35_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBK6909222.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="LNE35_000028"
     CDS             31954..32538
                     /locus_tag="LNE35_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBK6909223.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="LNE35_000029"
     CDS             32540..32959
                     /locus_tag="LNE35_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBK6909224.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="LNE35_000030"
     CDS             complement(33240..33524)
                     /locus_tag="LNE35_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBK6909225.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="LNE35_000031"
     CDS             complement(33876..34586)
                     /locus_tag="LNE35_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBK6909226.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="LNE35_000032"
     CDS             complement(34583..36055)
                     /locus_tag="LNE35_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK6909227.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="LNE35_000033"
     CDS             36193..36666
                     /locus_tag="LNE35_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6909228.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="LNE35_000034"
     CDS             complement(36819..37370)
                     /locus_tag="LNE35_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6909229.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="LNE35_000035"
     CDS             37475..38005
                     /locus_tag="LNE35_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBK6909230.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="LNE35_000036"
     CDS             complement(38488..38931)
                     /locus_tag="LNE35_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6909231.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="LNE35_000037"
     CDS             complement(38947..40107)
                     /locus_tag="LNE35_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6909232.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="LNE35_000038"
     CDS             40614..42314
                     /locus_tag="LNE35_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909233.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="LNE35_000039"
     CDS             complement(42416..43249)
                     /locus_tag="LNE35_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBK6909234.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="LNE35_000040"
     CDS             complement(43553..45718)
                     /locus_tag="LNE35_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6909235.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="LNE35_000041"
     CDS             45978..48272
                     /locus_tag="LNE35_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK6909236.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="LNE35_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="LNE35_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK6909237.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="LNE35_000043"
     CDS             48923..49930
                     /locus_tag="LNE35_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK6909238.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="LNE35_000044"
     CDS             49952..50971
                     /locus_tag="LNE35_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6909239.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="LNE35_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="LNE35_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK6909240.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="LNE35_000046"
     CDS             complement(52729..54150)
                     /locus_tag="LNE35_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6909241.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="LNE35_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="LNE35_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK6909242.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="LNE35_000048"
     CDS             complement(56561..57817)
                     /locus_tag="LNE35_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBK6909243.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="LNE35_000049"
     CDS             58397..59698
                     /locus_tag="LNE35_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK6909244.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="LNE35_000050"
     CDS             complement(59767..60366)
                     /locus_tag="LNE35_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6909245.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="LNE35_000051"
     CDS             60620..61501
                     /locus_tag="LNE35_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909246.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="LNE35_000052"
     CDS             61514..63124
                     /locus_tag="LNE35_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBK6909247.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="LNE35_000053"
     CDS             complement(63201..63860)
                     /locus_tag="LNE35_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6909248.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="LNE35_000054"
     CDS             complement(64143..64808)
                     /locus_tag="LNE35_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBK6909249.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="LNE35_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="LNE35_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBK6909250.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="LNE35_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="LNE35_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBK6909251.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="LNE35_000057"
     CDS             complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="LNE35_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBK6909252.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="LNE35_000058"
     CDS             67122..67442
                     /locus_tag="LNE35_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBK6909253.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="LNE35_000059"
     CDS             complement(67554..68423)
                     /locus_tag="LNE35_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6909254.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="LNE35_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="LNE35_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="LNE35_000061"
     CDS             complement(69819..70328)
                     /locus_tag="LNE35_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909255.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="LNE35_000062"
     CDS             complement(70503..72638)
                     /locus_tag="LNE35_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6909256.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="LNE35_000063"
     CDS             complement(72721..74376)
                     /locus_tag="LNE35_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6909257.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="LNE35_000064"
     CDS             complement(74378..74692)
                     /locus_tag="LNE35_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK6909258.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="LNE35_000065"
     CDS             74862..75122
                     /locus_tag="LNE35_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBK6909259.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="LNE35_000066"
     CDS             75136..75450
                     /locus_tag="LNE35_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBK6909260.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="LNE35_000067"
     CDS             complement(76180..76734)
                     /locus_tag="LNE35_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBK6909261.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="LNE35_000068"
     CDS             complement(76860..78788)
                     /locus_tag="LNE35_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBK6909262.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="LNE35_000069"
     CDS             complement(79094..79810)
                     /locus_tag="LNE35_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK6909263.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="LNE35_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="LNE35_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBK6909264.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="LNE35_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="LNE35_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBK6909265.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="LNE35_000072"
     CDS             complement(83280..84740)
                     /locus_tag="LNE35_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909266.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="LNE35_000073"
     CDS             complement(84730..86025)
                     /locus_tag="LNE35_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6909267.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="LNE35_000074"
     CDS             complement(86357..87034)
                     /locus_tag="LNE35_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBK6909268.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="LNE35_000075"
     CDS             complement(87064..88155)
                     /locus_tag="LNE35_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBK6909269.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="LNE35_000076"
     CDS             complement(88256..88690)
                     /locus_tag="LNE35_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6909270.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="LNE35_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="LNE35_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK6909271.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="LNE35_000078"
     CDS             complement(90728..92623)
                     /locus_tag="LNE35_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6909272.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="LNE35_000079"
     CDS             complement(92681..94456)
                     /locus_tag="LNE35_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK6909273.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="LNE35_000080"
     CDS             complement(94506..96002)
                     /locus_tag="LNE35_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6909274.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="LNE35_000081"
     CDS             complement(96196..97818)
                     /locus_tag="LNE35_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK6909275.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="LNE35_000082"
     CDS             complement(98267..99580)
                     /locus_tag="LNE35_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK6909276.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="LNE35_000083"
     CDS             complement(99825..100049)
                     /locus_tag="LNE35_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909277.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="LNE35_000084"
     CDS             complement(100069..100491)
                     /locus_tag="LNE35_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBK6909278.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="LNE35_000085"
     CDS             complement(100635..101345)
                     /locus_tag="LNE35_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909279.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="LNE35_000086"
     CDS             complement(101363..101860)
                     /locus_tag="LNE35_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6909280.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="LNE35_000087"
     CDS             complement(101897..102349)
                     /locus_tag="LNE35_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6909281.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="LNE35_000088"
     CDS             complement(102484..103089)
                     /locus_tag="LNE35_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK6909282.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="LNE35_000089"
     CDS             complement(103539..104159)
                     /locus_tag="LNE35_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6909283.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="LNE35_000090"
     CDS             complement(104161..105030)
                     /locus_tag="LNE35_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBK6909284.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="LNE35_000091"
     CDS             complement(105053..105460)
                     /locus_tag="LNE35_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK6909285.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="LNE35_000092"
     CDS             complement(105479..106768)
                     /locus_tag="LNE35_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6909286.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="LNE35_000093"
     CDS             complement(106795..107097)
                     /locus_tag="LNE35_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6909287.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="LNE35_000094"
     CDS             complement(107122..109215)
                     /locus_tag="LNE35_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6909288.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="LNE35_000095"
     CDS             complement(109704..110468)
                     /locus_tag="LNE35_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBK6909289.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="LNE35_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="LNE35_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBK6909290.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="LNE35_000097"
     CDS             complement(111473..112549)
                     /locus_tag="LNE35_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBK6909291.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="LNE35_000098"
     CDS             complement(112573..113166)
                     /locus_tag="LNE35_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBK6909292.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="LNE35_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="LNE35_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBK6909293.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="LNE35_000100"
     CDS             complement(114836..115135)
                     /locus_tag="LNE35_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBK6909294.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="LNE35_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="LNE35_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBK6909295.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="LNE35_000102"
     CDS             complement(116890..117801)
                     /locus_tag="LNE35_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK6909296.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="LNE35_000103"
     CDS             117921..118778
                     /locus_tag="LNE35_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6909297.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="LNE35_000104"
     CDS             complement(118965..119327)
                     /locus_tag="LNE35_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBK6909298.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            119673..121112
                     /locus_tag="LNE35_000105"
     CDS             119673..121112
                     /locus_tag="LNE35_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909299.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="LNE35_000106"
     CDS             complement(121119..123671)
                     /locus_tag="LNE35_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBK6909300.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="LNE35_000107"
     CDS             complement(123785..125116)
                     /locus_tag="LNE35_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6909301.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="LNE35_000108"
     CDS             complement(125082..126560)
                     /locus_tag="LNE35_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6909302.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="LNE35_000109"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="LNE35_000109"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK6909303.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            129170..130597
                     /locus_tag="LNE35_000110"
     CDS             129170..130597
                     /locus_tag="LNE35_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909304.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(130689..131054)
                     /locus_tag="LNE35_000111"
     CDS             complement(130689..131054)
                     /locus_tag="LNE35_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK6909305.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(131051..131416)
                     /locus_tag="LNE35_000112"
     CDS             complement(131051..131416)
                     /locus_tag="LNE35_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK6909306.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(131608..131856)
                     /locus_tag="LNE35_000113"
     CDS             complement(131608..131856)
                     /locus_tag="LNE35_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909307.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            131855..131983
                     /locus_tag="LNE35_000114"
     CDS             131855..131983
                     /locus_tag="LNE35_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBK6909308.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            132040..133734
                     /locus_tag="LNE35_000115"
     CDS             132040..133734
                     /locus_tag="LNE35_000115"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBK6909309.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            133738..133857
                     /locus_tag="LNE35_000116"
                     /pseudo
     CDS             133738..133857
                     /locus_tag="LNE35_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            133834..134007
                     /locus_tag="LNE35_000117"
     CDS             133834..134007
                     /locus_tag="LNE35_000117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909310.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            134385..135518
                     /locus_tag="LNE35_000118"
     CDS             134385..135518
                     /locus_tag="LNE35_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBK6909311.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            135549..135869
                     /locus_tag="LNE35_000119"
     CDS             135549..135869
                     /locus_tag="LNE35_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBK6909312.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(135898..136362)
                     /locus_tag="LNE35_000120"
     CDS             complement(135898..136362)
                     /locus_tag="LNE35_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6909313.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(136494..137747)
                     /locus_tag="LNE35_000121"
     CDS             complement(136494..137747)
                     /locus_tag="LNE35_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6909314.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(137776..138093)
                     /locus_tag="LNE35_000122"
     CDS             complement(137776..138093)
                     /locus_tag="LNE35_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6909315.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(138119..138439)
                     /locus_tag="LNE35_000123"
     CDS             complement(138119..138439)
                     /locus_tag="LNE35_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6909316.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(138903..140318)
                     /locus_tag="LNE35_000124"
     CDS             complement(138903..140318)
                     /locus_tag="LNE35_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6909317.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(140335..141651)
                     /locus_tag="LNE35_000125"
     CDS             complement(140335..141651)
                     /locus_tag="LNE35_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6909318.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(141801..142499)
                     /locus_tag="LNE35_000126"
     CDS             complement(141801..142499)
                     /locus_tag="LNE35_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBK6909319.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(142788..143744)
                     /locus_tag="LNE35_000127"
     CDS             complement(142788..143744)
                     /locus_tag="LNE35_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6909320.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(143857..144690)
                     /gene="msrA"
                     /locus_tag="LNE35_000128"
     CDS             complement(143857..144690)
                     /gene="msrA"
                     /locus_tag="LNE35_000128"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK6909321.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(144865..146499)
                     /locus_tag="LNE35_000129"
     CDS             complement(144865..146499)
                     /locus_tag="LNE35_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909322.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(146486..148354)
                     /locus_tag="LNE35_000130"
     CDS             complement(146486..148354)
                     /locus_tag="LNE35_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909323.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            148320..149957
                     /locus_tag="LNE35_000131"
     CDS             148320..149957
                     /locus_tag="LNE35_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909324.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(150102..150842)
                     /gene="truA"
                     /locus_tag="LNE35_000132"
     CDS             complement(150102..150842)
                     /gene="truA"
                     /locus_tag="LNE35_000132"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK6909325.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(151033..151668)
                     /locus_tag="LNE35_000133"
     CDS             complement(151033..151668)
                     /locus_tag="LNE35_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBK6909326.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(151815..153056)
                     /gene="sstT"
                     /locus_tag="LNE35_000134"
     CDS             complement(151815..153056)
                     /gene="sstT"
                     /locus_tag="LNE35_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBK6909327.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            153254..154252
                     /locus_tag="LNE35_000135"
     CDS             153254..154252
                     /locus_tag="LNE35_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909328.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            154265..156283
                     /locus_tag="LNE35_000136"
     CDS             154265..156283
                     /locus_tag="LNE35_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBK6909329.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(156343..157059)
                     /locus_tag="LNE35_000137"
     CDS             complement(156343..157059)
                     /locus_tag="LNE35_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBK6909330.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(157286..157828)
                     /locus_tag="LNE35_000138"
     CDS             complement(157286..157828)
                     /locus_tag="LNE35_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK6909331.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(157833..158060)
                     /locus_tag="LNE35_000139"
     CDS             complement(157833..158060)
                     /locus_tag="LNE35_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6909332.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(158139..160019)
                     /locus_tag="LNE35_000140"
     CDS             complement(158139..160019)
                     /locus_tag="LNE35_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK6909333.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(160208..160822)
                     /locus_tag="LNE35_000141"
     CDS             complement(160208..160822)
                     /locus_tag="LNE35_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6909334.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            160964..161524
                     /locus_tag="LNE35_000142"
     CDS             160964..161524
                     /locus_tag="LNE35_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6909335.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(161829..162143)
                     /locus_tag="LNE35_000143"
                     /pseudo
     CDS             complement(161829..162143)
                     /locus_tag="LNE35_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(162397..164032)
                     /locus_tag="LNE35_000144"
                     /pseudo
     CDS             complement(162397..164032)
                     /locus_tag="LNE35_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(164587..165150)
                     /locus_tag="LNE35_000145"
     CDS             complement(164587..165150)
                     /locus_tag="LNE35_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBK6909336.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(165165..166208)
                     /locus_tag="LNE35_000146"
     CDS             complement(165165..166208)
                     /locus_tag="LNE35_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909337.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(166341..166745)
                     /locus_tag="LNE35_000147"
     CDS             complement(166341..166745)
                     /locus_tag="LNE35_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK6909338.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(166978..167613)
                     /locus_tag="LNE35_000148"
     CDS             complement(166978..167613)
                     /locus_tag="LNE35_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBK6909339.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(167879..168931)
                     /locus_tag="LNE35_000149"
     CDS             complement(167879..168931)
                     /locus_tag="LNE35_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6909340.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(168944..169882)
                     /locus_tag="LNE35_000150"
     CDS             complement(168944..169882)
                     /locus_tag="LNE35_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK6909341.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(170333..170983)
                     /locus_tag="LNE35_000151"
     CDS             complement(170333..170983)
                     /locus_tag="LNE35_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909342.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(171083..171592)
                     /locus_tag="LNE35_000152"
     CDS             complement(171083..171592)
                     /locus_tag="LNE35_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBK6909343.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(171793..172662)
                     /locus_tag="LNE35_000153"
     CDS             complement(171793..172662)
                     /locus_tag="LNE35_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6909344.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(172682..173803)
                     /locus_tag="LNE35_000154"
     CDS             complement(172682..173803)
                     /locus_tag="LNE35_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBK6909345.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(173805..174200)
                     /locus_tag="LNE35_000155"
     CDS             complement(173805..174200)
                     /locus_tag="LNE35_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6909346.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(174219..175025)
                     /locus_tag="LNE35_000156"
     CDS             complement(174219..175025)
                     /locus_tag="LNE35_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6909347.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(175018..175806)
                     /locus_tag="LNE35_000157"
     CDS             complement(175018..175806)
                     /locus_tag="LNE35_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6909348.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(175820..176296)
                     /locus_tag="LNE35_000158"
     CDS             complement(175820..176296)
                     /locus_tag="LNE35_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6909349.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(176368..179025)
                     /locus_tag="LNE35_000159"
     CDS             complement(176368..179025)
                     /locus_tag="LNE35_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6909350.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(179044..179448)
                     /locus_tag="LNE35_000160"
     CDS             complement(179044..179448)
                     /locus_tag="LNE35_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HBK6909351.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(179680..179937)
                     /locus_tag="LNE35_000161"
     CDS             complement(179680..179937)
                     /locus_tag="LNE35_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBK6909352.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(180062..180394)
                     /locus_tag="LNE35_000162"
     CDS             complement(180062..180394)
                     /locus_tag="LNE35_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909353.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        54176 a        37220 c        30704 g        58887 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
   128821 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
   128881 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaacaa
   128941 ataaaatgac tgtccttcat tcactaaaca tatttttatt tttttatatt gttaaaaatt
   129001 cacaatttat tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt
   129061 tttaacggca tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt
   129121 cataatattt cccatattgt gcaaaaaaat acaaggcgga ggctctacta tgattgaaga
   129181 atatattgag aaaaatattt taagacagct gtttttatgt gggcaattct atgtaaataa
   129241 agaagtcaat ctagaaaagc tctccaatct cctgcatgtt tgcaaaacca ctttactcaa
   129301 tgatatcaat aacataaaaa aagaatttga agaacagatc gcttatacac atcgggaaaa
   129361 ggaccgttac actttatatt tttcagaaca tatacctcga tgcaagatca tgcagcaatt
   129421 gtctcaaaat tcgttgtttt taaaaacttg tctgctttat ttggaagaag atgaacctga
   129481 ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa
   129541 acaagtatta gcttacttta atgactgcgg gatcgaaatc gaccgctact cccctcgatt
   129601 cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg tatcgtttag gcggttttaa
   129661 ttcttgggag ctacctgaat cgttttttga acgtgccgat cgtttcattg aatccgtaac
   129721 ggaaaatagt ggcagatttt atgataaaga aaataaagaa atcttatcga ttggttttgc
   129781 tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga
   129841 aatcaaaaaa cgtcctattt acaattatgt cgaatcagca tgggaaaaca ctgatttcca
   129901 aacctattat aaaaaagaag aattcgcctt tattttgaca ctattcaatc tttgtaatta
   129961 tggtttccat tcttatcagt tgattgcgga agactttcaa cagctacacc aagtgtttat
   130021 tgacaatact cccgaaataa aagaactagt tgctaccttt gaatctcact tcaatcaaga
   130081 attattcgga aaccaaccgt tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga
   130141 taactatcaa ttattcatgc cagaaaaatt ttatctttta aatgaggaac agacaaattt
   130201 atataaagaa gtccagacaa ttttttccag ctggtccagc cagttacctt atgatctacg
   130261 gttgaatcct aattgcatgc gggcttttgt gatcgaattg agcggaattc tccgtctgac
   130321 aaaagaacat ctgactatct atatcgtcac aaattcggat gttcattatc tgatttatcg
   130381 ggaagccttg gaagctgtaa ctactttcga ttttcaagtt gcaccaacaa tttattcaag
   130441 tatatctgat ataaagaagt atgcgcagca gtcctcaaac cgggtattat gtgaacgtac
   130501 attatataca ccagatgcgg tccaatatga aaatattatt cctatttcga tcaatacgat
   130561 cgatcgagct atcatttcag ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc
   130621 acagcctctt atttgttgaa gatgcccatt tctgtaacaa tctcttgata cacgtcaggc
   130681 tcacaacctc acttattttg gacatctagg ggtccgatct ctttatttaa aaaataagaa
   130741 atgatggttc ggataactac caacgccgct agttggaaaa tatcctgcaa ggtaggtttg
   130801 ataatcgatt cgatgatccc agctgaaaca agaatttcca aacttagaag tatatagcct
   130861 cccaacatct ttttaatagc attgttctcg cttactcggt cgctttgcac ttttgtatgg
   130921 acaaataaat atttcaaaaa atccttgatt gatagacaaa caccccaaac caataaacag
   130981 atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat atggaagaag aaactgcatc
   131041 aactctgtcg tcatcctacc tctcctcttc tttgtttttc tttgtcttct atttcctgat
   131101 tcaagaaata agaaatgact gttcgaatga taataataag tgcaagcttc aaaatatctg
   131161 tccaagtcgg cttgataact gactcgatga tatcagcaac gactagtact tccaagctta
   131221 acaaaatata acttgctagc agttttcttg tatctgcatt acgagtatta cgttctgcat
   131281 agtttttctt gacatgagac caatcaaaaa gtttgaatag aacaagaacg atcccgacaa
   131341 ttaagatacc gatagaacaa atatttaaaa tagcaatgat ccattctaga aattcaatta
   131401 aatttgagcg aaacatatac gctacctact ttcttttaat ttgttcctat tcttttattg
   131461 taaaagaaag ttaccttgtt cgtctattta aaagttgttc taatgataac ggttcgctta
   131521 aatttttctt tttcactctc ccatttttct ccctccatcc ataaatcgtg gaagtatcca
   131581 aaacaacgta gcaagttaaa ataaggctta gtccgtcatc agcttttcaa agaagcactg
   131641 tctcggtgct ttttttgtac ccaaatttct aaaaatagaa ccatacacct cctcaaatct
   131701 tttcttctta cctaccctat ttttatttac aggaaaatca gacacttctt cttttttgtc
   131761 aaaatttcct ttttcttctc gtcctatcat ttggcattct aataaaaacg aactatcctt
   131821 ggatatagaa agagaaaaca aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg
   131881 catcggttta ggtttgcttg cccttggcgc tgcatatcta ggaaaaaaga caggactatt
   131941 cgaagatgac agtcatttat atgatgaatt cgaatcaatc tagcatccta tttagttgaa
   132001 tcactacgga catacttgga agacattgga ggaatcacaa tgagaaaaag taaagcaata
   132061 atgattggtg ccggtattgc caatatggca gcggctgttt atttgattca agaaggcaaa
   132121 tggaaagggg atcagatcac cttttattca ttggatgatc acggttcaaa tgatggtgca
   132181 ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc atccattaga aaaccaaaaa
   132241 ggctatattg ctcgaggcgg tcgcatgctt aattaccgta cctatgtaga tttgatggat
   132301 ctgttaagtc gaataccttc tgttactgag ccagggatga ctgctgaaga agatacgaga
   132361 gattttgatg caaaacaccg aacctttgac aaagcacgtt tgatggaagg tggcaaagga
   132421 atcattgatg gcggaaaact gggattcaat aataaagacc gtattcttct gactaaactg
   132481 attgctatgc ctgattcaga agaagaaaaa ctagacaata tcactatcga agaatatttc
   132541 aaagatgatc cacacttttt tgaaacaaac ttctggttta tgtgggaaac aacatttgct
   132601 ttcagaacac gtagttctgc tcaagaattg cgtcgctata tgcatcaaat gatttatgaa
   132661 ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac gttacaatca atacgaaagt
   132721 attatgcttc ctttgatcga gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc
   132781 cgtgtcactg actgggaatt caaagaaaca ccgatgcaag atgaaatcac tgtgactggt
   132841 ttgaaaatta ccaatacact gactcaagaa gaagaatacg ttgtggtcga tgaagataca
   132901 gcagttattt tcactaatgg gtccattaca gattcagcta ctttaggaga ttacgaaaca
   132961 ccagctgtag aaaatatgga ttatggtgca gcttctagtc tctggaaaaa agccagtgaa
   133021 cgtttctaca acttgggaaa tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg
   133081 gtcagcttta ccctgacgac gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg
   133141 actcaagaac caggaaatgc cttgaattca tttatttcaa ccacaccgat cacgccgtta
   133201 ggccaaaaag atgtcaatat gtctattgtc gtgcatcatc aaccgcattt tacgtctcag
   133261 aaaccaaatg aaacagttat ttggggttat ttcctttatc caagacgacg tggcgaattt
   133321 gtggataaac agtatattaa aatgaatgga aaagaaatgc tagaagaact gattggacaa
   133381 ctttcaaaag tagatcctgg accagtgaat attcgtgaaa aagaaacaga aattttcgac
   133441 agcgtaataa ataatatccc tgtctatatg ccttatgctt ccgcgttgtt caataatcgt
   133501 gccaaaagcg atcgaccaaa agtcattcct aaacattcga ccaatctggc ctttacagga
   133561 gaatttgtcg agcagcctta tcagatgatc tttactgaac aaagtgctgt acgttctgga
   133621 gaaatcgctg ctttccattt tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt
   133681 tacgacaaag atattcctac tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga
   133741 ggttgtgaaa aagctgccct gcatcaagta ataagaacaa ccaggctaaa acagcttctt
   133801 atgtttttga tttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttacca
   133861 agaggttatg aaaaagctgc cctgcatcaa gtaataagag cagccaagct aaaacagctt
   133921 cttatgtttt tgacttggtt agacttatta ccgcagatgc aagcttttga acaccgttta
   133981 ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg
   134041 ttcctcacgt ttttgcataa atttgactta ataccgagaa actgatccct tctgtcataa
   134101 cctcttttta tttatgtctg tttctttttg aaaacaacta ttttttcaac gtttactctc
   134161 ttgccaatcc aacatcatct aacggataca actatttcaa tgattaccat tacactaaaa
   134221 aatcggaaac acacctctcc gaacacaaat acataagact atagaatgtt tcatttaaga
   134281 aaaaaagcgt ttcattatca aaaaggtaag atctccttta aaatgaaatt atctgtagta
   134341 ctaataatgc agacaatttt atgaatctga aaggagcatc tccaatgaaa gcagtcactt
   134401 ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga tccaaaaata ttagaaccaa
   134461 cagatgcaat catccgtatc acagcaacag ccatttgcgg ctccgatctg catctttacc
   134521 atcatggtga agcagttttg gaaaaagggt atgttgtagg acacgaacca atgggaatcg
   134581 ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt
   134641 ttaatattag ttgtggtcat tgtcatttct gcctgaatca catggaaagc caatgtgatg
   134701 aatcaaatcc agatcagtta tatggcgggc ttttcggatt tggcaagttg aacggtaatc
   134761 attggggtgg tcaagcggaa tatctaagag ttccttatgc agactctact tcatttgttg
   134821 taccagataa tgatcttcct gatgaaaaag ttttatttct atcagatatt ttgcccacag
   134881 cttattggag cgttgaaaac gcaggagtaa aaaaaggcga tacagttgtg attctaggtt
   134941 ctggtccagt cggtctattc gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa
   135001 tcgcagtaga tccagtacag caccgtctag ataaagcggc taattacaat ggggttgaaa
   135061 cttatttctt agaagacacg gcacaagctg gaacagaatt gtatgaactg gctaaaggtg
   135121 gcgcagatgt cattatcgat tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag
   135181 cgaaaaattt agtaagctta caaagcggca caatctctcc attacagatg gcatcgcaag
   135241 ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta tatgactcca gcttcttctt
   135301 acccgctgca agaatttttt atgcgggata tcgctgtcaa gcatggacag gcaccaatta
   135361 tccatctaat gccaaaaatt tacgcaatga ttgcagaagg actattcgat cctacacaaa
   135421 tcattactca ttcgctgcca ctatcagaag cagcacaagc atatgagatc tttgacaaaa
   135481 aagaagataa aaatataaaa gttgttttga aaccttgaat tcagtaaaaa atacaggtga
   135541 ttttctttat gcccaaaatc aaacgaaata cccatgagat cgaaatagga aaagaagaag
   135601 atatcgaaat acgaggacgt cgcttccgct tctatttcca aaatcgttac acattgatct
   135661 cactggctgt cgatttatta accgggattt tctatatttt tggaagcctt gcttccttaa
   135721 ctaaaatccc cgatcgatat ggcacctatt tttatttggc tggtgctatt ttcttgacga
   135781 ttcgtcctat tctcagaatc gtaaggaaca tttttattta cgacgaaaaa aaagcaatga
   135841 agagaacaag caaaaaaaaa gaggcatgag aatttttcaa atctcatgcc tcttttttca
   135901 ccctatcgta tcactttgat acagacgagc ggtcgtccca ctttcttcct tgaccattgt
   135961 caaaaatgtc cactcgtatc gttcataaaa cgtggtatga tctgtaatta agtaaagttt
   136021 ttcgtaccca agttgatgca tttcttttct gatttccgct aatatccgct tggctatccc
   136081 ctgcttacgc ttttccggtt caacaaacag ggcacaaaga tttggtgtca gatcagggcg
   136141 gttatggaag tcgttttcaa tgacgcccgc tcctccgacg atggtttctt tttcatctaa
   136201 gaccacatac cattgaggaa tactttcctt attagcgatt ccagtacgaa tactttcctc
   136261 atacacaaca gcaggaactc cccattttga tgaaaaccaa ttcgcgcttt gttttactaa
   136321 ttctgggtgt tctcttaatt tgatccagtt atatgtaacc aatgtttaaa cctccgacta
   136381 aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa attagcaatg acaacaatag
   136441 tgatgtaaaa aaaaaaacgg aaaacaatca cttgttctcc gttttttgat taatcaagca
   136501 tctaaagctt cttttcttct ttcttggatc gtagctacct tgacgaacgg tagatagatc
   136561 aaaatagaaa gaaccaaatt gacagcggct agtacaccac ctgcaatact ttttgttgcc
   136621 aatacaccgc caataattgg tggagttacc catggaggca taacggtagc tgctggcact
   136681 aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga ccattggtgt caaaataaat
   136741 ggaatgaaca tgatcgggtt caacacgata ggtaacccaa acatcgttgg ttcattaata
   136801 ttgaagacac ctggcgcaat cgacaagttt gttacaacca tatatggttt gtttttacgt
   136861 cctactaaat aaatagctaa cagtaaacct aaagttgcac ctgttcctcc taagttgaca
   136921 aagctgtcaa agaaaggttt gttcacgata aacggtgcgc tatgtccagc tgcgattgca
   136981 ttaacgtttg cttcaatggc tggcgcattg attgtttgca tcaaaggatc gaccatattc
   137041 gctccgtgta aaccaaagaa ccataagaat ggtgtgataa atgcaagcag taaggcagat
   137101 ggataagagt ttgcaagtcc cataaatggt tcttggacca ctgtatagaa agaacctacg
   137161 atatcatcaa caccgaatgc agcaaaaatt gcagcaacta aagcaaacgc agagatagtg
   137221 atcattgctg gtagtaatga agcaaatgct tttgcaactg ccggtggcac accatcaggc
   137281 attttgatga tcaatttttc atttccgctt aaacgtacaa agatttctgt tgaaataatc
   137341 ccaacaataa tagcaataaa caaaccgttt gagctcatac ctaatgctcc gcctaaagcg
   137401 aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt cttttcggta gccgttagct
   137461 aaatgataag caagcaagaa agcaatcaag acagaaagaa tggcaaatgt accgttccaa
   137521 gcagcagccc cgaatccttt ccatgattca ccaccaaaaa ttgagttcat caattcttgg
   137581 aacccgggaa tcggtaagtt attgatcatt actgctaatg ctcctaagat cattaacggc
   137641 atactcacca taaatgagtc ccgaatcgca actaaatgcc gttgtgcacc aatttttgat
   137701 gctacaggta taaaatgttt ctccataaat gcaacaaatt tatccataaa tcttccctct
   137761 tttctttact gtagattacg cgttcaatcg acgatacaga ttaatgatct cttttgctaa
   137821 atctgtaaaa gctatagatg tcatcagatg atcttgactg tggaccgtca aaagcgtgac
   137881 ttcaacgtga tttccttgtg cttcttgtgt cagcatctct gtttgagagt gatgtgcttg
   137941 gactaaagat tcctctgcat ctttgatttt ttcatctgct aaagtgaaat cacctttttt
   138001 cgcagcagca atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc
   138061 catgatggct tctagatttt tttgatcttc catttattgt atcctccgat aatttttatt
   138121 atttgtttat tagtttttct gcttggtcga gtactttttc accattcatc atgccatagt
   138181 cagccatatt gattacatct aaaggaatgc ctttaggtgc tagcttttca gcaaattgat
   138241 tttgcataaa gcgtacttgc ggtcctaaaa gtaaaacgtc tacattttta gattctagtt
   138301 gattatccgc atctgaagca gatacagcaa agatgtctgc ttccattcct tttgcttctg
   138361 ctgccttttg catttttgtg actaggagac ttgtactcat tccagctgaa catacaagca
   138421 tgattgtttt cttttccatt ttaaacacgt tcctttctat acttacactt gtatataagc
   138481 aagtagcgtg ccaactttaa aagcgcttta aaaagttgat atcatcacat ttaaggtcta
   138541 taccaagttg catacacact aaaaaagaaa gcgcacacta aacacacaaa ttaattttta
   138601 ttaccttttt tcttttaacg tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt
   138661 tttcaaataa acggtattca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
   138721 acataagatg ttgtttttac tttgctattc ttattacttg atgcaggaca gctttttcac
   138781 aacctctcaa taaacggtgt tcaatcagct gaccctgggt attagctcaa aacgctggat
   138841 tcacaacctc ttttatttca aataaccagt aaatacaatg aacatctaca aaaaacttct
   138901 atttatttat agatttcgtc aggcaaatcc aattctctct tatcaatcaa agtctgatac
   138961 cacgaagcag attttttcaa tgttctgctt cgttcatcgt tcaattcgat tcgaactaat
   139021 ccatatcgat tcttaaatgc attcattggt gacacacagt cagtgaatgc ccataacata
   139081 tagccttcgc aatttgcgcc ttcttctgtt gctttgatca agtaatgcaa atgctgtccg
   139141 ataaattcga tacggtaatc atcttgcacg attcctttct catccatata ctgttcttca
   139201 tattctcttc ccataccgtt ttctgttatc agccaaggga tattatgata attttctttt
   139261 agatacatgg ctatatcata catgattttc ggatagattt cccatcccct ggaattattc
   139321 attttcttcc caggtaaatc aaagacttca taatatcgtt caggatggaa aggtgtctct
   139381 tcattccatt gataacgagg ggcgcgaacg cgtttaggat aatattgatt gattccaagg
   139441 aaatctactg tatgatgctg aatcgtttct aaatcctctg ttgcaggatc gaaataaatc
   139501 gattgttttt gacacaactc caggaactct tctgtgtagt atccttttac catcggatca
   139561 aaaaatactc gattataaaa caaatcatac atataagcag ctttttggtc ttctggagaa
   139621 ttggatcgag catagaccat ttcaggattt aaaatacagc caatccttcc accataattt
   139681 ccgctgcggt aaatctctac tgctttagcc gttgctaaaa ctttatgata attccacatc
   139741 atccactttt ttgtgttttg ttcatgcggc cagcgaacag catccaaata gcaacgcgtt
   139801 tggggaacga ttggttcgtt gaatgtaaac cagtttttta ccctatcacc gtaacgatca
   139861 aaggcaattt gagcatactt agcataaaaa tcaactactt ttcttgaaga ccagccatca
   139921 tatttttcca aaagaaaagc tgggacttca taatgttcca aacaaatcat cggttctacg
   139981 tctgcttcga tcaaagagtc gatcatttca cttatatgct gagcatattc ttcatctact
   140041 atacagttct cataatcagt aaaaaaacga gcccaattga tagaagtccg ataatgtgtc
   140101 agatggatgg atttcatcag atccacatcc tgctggtatt gttccataaa attagttgct
   140161 acggctgggc catacccatt atgccaaaca aaatgttcat ttttatacca gctatctata
   140221 aacgaatcct gtccttcttt ttttcctttc caaccttccg tttgccaagc agaagatgca
   140281 gcaccaagaa taaaattttt tggaatagta attttcatga tttcctcctg ttttttagat
   140341 cccatcattt tccacacgat ttgctacttt tacaaatggc agatagatca aaatagaaac
   140401 aataatacaa acaagttgcg taataactgc accgatattt cctgctgttg ccagataagc
   140461 attgacgatc ggcggcattg gccaagggac cattacaact gctttcgccg caaatcctaa
   140521 agcggttgca aaataaccaa tcgttccagt gataagaggc gttaagataa acggaatagc
   140581 taaaattgga ttcagcatga tcggcatacc aaaaataact ggttcattga tgttgaagat
   140641 cccaggagca agggataact tggtgatttg tttcatatct tcccgttttc caactaacaa
   140701 tactgcaatc agtaaaccga tcgtgactcc agacccgccc atactcatat acatatccca
   140761 aaatggcata gtaataatgt tcggtacttc ttttccagct tcaaaagctt ccgtatttac
   140821 agcaattgcc gctaaaagca acggttcccg tatcggcttc accatttgat ttccatggat
   140881 accaatcacc caaaagactt gcgcaataaa catcaatagc aaaatacccg gtaacccttg
   140941 tacgacacct tctaatgggc gttgtacgac attgtaaatg atatcataaa ggtacattcc
   141001 tgtaagttct ttgatgataa atcccgcagc cgcgataccc gatacagtca gaatcgtcgg
   141061 gaataaagca gaaaaactag cagaaacatt cgttggcacg gtatcaggca ttctcagctg
   141121 tagtttcttc tgtttcctca accagcaata aagttcaata gaaaggatgg agaccaacat
   141181 tcccaaaaat aaccctttcg tatctgtata ttgtttagcc aatacgtttt ctactataac
   141241 agtctgattt tcattcacta attcataaac agttggatta acagataaat aagacatcac
   141301 tgcgatgata ccagggaata caccatccac tttattcagc ttggctaatt cgatccctat
   141361 cagaaaaaca gcatagatgg tcaagaaact tagtgtgaca tagctgatgg cagatgcgat
   141421 aggctttaat tcttctaaaa aacgcaagaa acttatctgt gctaagccat tttccgagtc
   141481 aaagaccatt gctgaaaaca atgtgccaaa agcacccgta ataattaccg gaatcaatgc
   141541 agcaaatgcg ttttttatca ccatgatata ccttaaatta ttgattctcg ttgccacgtt
   141601 ccctaaaaca ttggctaatt tatccataaa actcattcca gatcccctca ttcctgcttc
   141661 ttctccttga cgtcatctct ttttgtctat tagtcagcgc ttacatttcg atgaacagaa
   141721 actatttact cctctttttt accaaatata aaacaaaaat tcatctgatg tatttatcat
   141781 aaaatacatc agatgaactg tcaattagtt tctttttctc ctattatttt ctgtaattct
   141841 ctccgattta ttgccatatg gacagtcatt gcatcaaacg cagcaagtgc attccgcctt
   141901 tcgatggcct gaacgatctc tgtatgaata tcgatcgtct cgattttcag atgctggata
   141961 tcataatttt tattaaacaa agcaattgat tcattaatga ttgggataat atgcagcaag
   142021 gctacgtttc cgctagcttc tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt
   142081 tcttgtgtgc cttctcgaaa agaagtttca atcttttgcg ttaatttttt catttggacg
   142141 atttgattct tcgttgcata ttccgctgcc aaacctgcca tcttaggctc caataaatat
   142201 ctcagttcaa ataagtctcg tatcatccga tcctgatctt caatcaaaga aaaaccgagt
   142261 gggtcattgg caatccctcg cttatcactg acaaatgtcc ccgctccttg ccgaacttca
   142321 aggatattcc tggaagctag agcccgcaca gcttctcgca ccgtgcttcg ccccacctct
   142381 aaataatcgg acaattcata ttcattcggc agtttatctc ctacttgcaa ctgcatttcc
   142441 tttaaatatc taaggatctt gtcagtagtt acttctacta gggttttatt ttcttccaaa
   142501 ccacatacct ccttttcatc tgatgtattt tttcgtaatt attataacac gtttgaatca
   142561 tctgatgatt ttttgtatac aaaattgatt aatccgttca tagacataca aacgaagaag
   142621 atatttacca taaaaaagac taggataaaa ttcactccta gccttcgatc atttttattt
   142681 tgaacgacaa aaaatatgat gttcaataac agacgatctt cattcgattc ttgatgtcat
   142741 cttttgtttt ttttaatata tggataaaaa gtttgattac atcgtttcta ttctagaatt
   142801 tggtcgattc tcaattcttc ttctggtgag aaatctaaat tatccatcat tcgaacactt
   142861 tcttgtaact gacttaaccg acttgctccg accagtacac ttgtaacaga tttttgtcgc
   142921 aaattccatg caagggccat ttgcgctaat gattgtcctc gtgatacggc gattgtttgc
   142981 agtgctttga ttttttccag agttgatcca acttgttctt ctgaaaggaa tggtatttcc
   143041 ttacgatgtg ctctagaatc ttcaggaata ccatgtagat aacgatttgt cagcaaccct
   143101 tgagcaagcg gactgaacgt gattgcccct agcccttctt cttctaatac tggagataga
   143161 tcgtcttcta gtagtgcacg agaaaacata ttgtagcgca tctgatgaat aatgaacggt
   143221 gcttcctttc tttttaaaat ttcagtcatt ttttttgtat cttctccatt atagttggat
   143281 ataccaatat ataatgcttt tccttgtctt acaagttgca tcaaagcttc tgctgtttct
   143341 tccaaaggtg tatcagggtc tgggcgatga tgatagaaga tgtcaacata atctagcccc
   143401 attctttgga gactttggtc acaacttgaa atgatggatt tacgagagcc ccattcccca
   143461 tatggccctt cccacatatg ataacctgct ttgctagaaa taatcagttc atcacggtag
   143521 gcttgaaaat cttctctaaa aatacgccca aagtttttct ctgcagctcc agcagggcct
   143581 ccataattat tcgcaaggtc gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc
   143641 tctctttgat tatggatagg atcataatca ccaaaatttt gccataatcc gagagataaa
   143701 atcggtaatt tcaaacctga atgaccggat ttacgatatt ccattttttc ataacgtgca
   143761 ctatctgcaa tatacaattt ttctcctcct catcaaatct ttttaaaacg ctttcttttt
   143821 tctattttta gtttactaca ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat
   143881 atttttttgc catttgtttt ttactcgttg atagatcaga aattgattac gtgcttgctg
   143941 gattttttta taacgtttcg gttgtttttt gtaaaattgc tgatgatagt tttctgctgg
   144001 ccaaaacgta ccggctggtt cgatttttgt gacgattggt tggtggaaag ttcctgaact
   144061 gtctagtttc tgttttgatt gttccgctaa ttctttttgt cgttcgactt gataaaaaat
   144121 cactggacga tattgcttcc ctctatcttg aaattgtcca aatgtatctg tgggatcagt
   144181 aatttgccaa tagattgtca gcaactcgct ataacttatt tctcttgtgt cataaatgat
   144241 ctccacagct tccacatgac ctgtgtaacc accacttact tgatcataat ttggtttttc
   144301 aacatgtccg cccgtgtatc ctgacaaaac agataggatc ccttttttcg attcaaacgg
   144361 ctcgaccata caccagaaac atccccctgc aaatactgca cgttcttgat agtcagcatc
   144421 ttgcatggca tgcttggaaa agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa
   144481 taaataaaag tccattacat caggcgtcag attcattcgg atagctaatg gacgtagtgt
   144541 tgcttctagt tttgataact gttctttaac ggatatattt tctcttatgt cattctttgt
   144601 ctgaatcaat acttttcgtt cccaatctcg tgtattcgga ttcaaaatca gattataaag
   144661 tgtttgaatg aatatttgtt cctcatccat agattctcct cctttccgaa gaagacaagc
   144721 tagtgacttt agctgtaatt tatcataaag tgaggaaata agcgattact tcatccccct
   144781 acagattttt cgtataaaaa agagagaatc cttatcgaca gattctcctt tttaatcaat
   144841 ttattttata aatgaaaagt taatttacct tttatggaaa actattttaa aaaataaaca
   144901 taaatatgca gttaatgtaa caagataaga aataatagtc gttactgtat aacttgtgct
   144961 tactttaata tcagcaactc ctttgactcc gcttatttgt accaatccat tagcattcac
   145021 ctgctccttt acaggttgat gatttatttc tacattcgtt cctaaataag catatacagg
   145081 aagtgtgatt acttggctct tctcattagt tactctatat atgatagaac taccagttgt
   145141 tttataagga aggtttaccc attgactgcc aatctttgct tcgtgtttta ttatatgctc
   145201 cttattctcc aatgaagcga taggagaata atcatataaa aaataatcca ttcctttagt
   145261 ggtatacgcc tctactgttt ctttgtttac aacaatatgt cccggctgag aaattatttt
   145321 ttgattgaaa ttttttttac tagaaacagt taaagtgatt aataacaaaa cagtcataaa
   145381 taaataaaat agttctcttg tcttattgta tcttctattt ttgaaaaata tcatcgttaa
   145441 tatcagagaa gagaatatta tttgcaagct taagatccgc caaggaaatt gtaacaattt
   145501 ggctggcgta ttttgcagaa gataccatgg caccaaacta gttgtagcta gccaagtcaa
   145561 acacgtacca taataaatct ttttttctat gtttgttaat tttttaaata aaatcagggg
   145621 aatacataaa gataacagac caactagtcc cattgagtaa gtagttaagt cgttgttcat
   145681 agcttcagtc agactatcga acacattaga tgcatgagca tataaatttg tcacgctagg
   145741 tttgttgatt ttttggtaga gcatttgttc aaacatcgga taccaaaaag cactcgtcag
   145801 aaccaatgtc attcctgccg cactaaataa ttttttaaat atagcaataa aatctgcatt
   145861 cttgaaacct ttagaaacca aacgaataaa aataaaaatg ataattaatg ctgtactcat
   145921 taatgttgat agtacatggc tgtacactag caaactcata ctcaatgcta gctttacaga
   145981 cggatacttt tcttcttttc cagagatgat ttgataagca taaaataata cagggataac
   146041 aagtgttatt gcgatagctt cggcaattgc ggaacgatag tagatgtcta ctgtccgata
   146101 agcagagaaa atatagagac aactaaataa aaatgccgct tttttttgtt ttaaaaaccg
   146161 ctcgccataa tgataagcta cgagtgttgt acacaagttc aataaccaaa cgtaaataat
   146221 atagcttaca atcaaatttt ttgtcatcca atataacatt acagctggaa aaaaagtcag
   146281 aaagggataa aaataattta caccatatcc agtataattg aatgtctcat aattaatcgg
   146341 actggtaaaa atagtcgata accccttgat tcgagataag tgaaaagaaa gatctccagt
   146401 ggtataaata ctctgtgtat ttttgaaaaa gatgaaagaa tatatactac taactataat
   146461 taatccactc aatattacta cttttttatt tattttcatt cttcttgttt atctcctttt
   146521 tagtcacata tcgagaatct ttcaacttat taggtgcaaa tcgtttaaaa cttgaataca
   146581 ccactaaaca ataagtcaga atactgatcc ataaagccat gagagcagtt ggagtatatt
   146641 ttgttgtaat ttctatctta ttttctccag catttacatc cacattcact aagccatgtt
   146701 ttttataaga gtcatttacg actttaccgt ttacttttac ctgtgtacct aaataagcat
   146761 atactggtac tgtcactttt tgctttttgt tgcttgccac tgtgtaagta atgttattat
   146821 ctgcagcttt atacttccct ttttcccacg attctcctac ttttacttca tgtttttcta
   146881 agtgaggcct ttcttttatc gcttctgttg gggcgtaatc gtataatcct cccatatctg
   146941 atgttgtata acgtgggagt gtttcttcat tgataacaat atgaccgtgt tttgattgga
   147001 taacttttgc gtaattttct tttgctgcta tagacgtgac gagtagaaac accacagtag
   147061 cgccaatgat taaccatgaa tattttttat tcatagtctt tttcataaaa acaagcgtta
   147121 tgatcattga agaaaaaaag atttggatac ctaagatacg ccaaggaaac tggagtaagc
   147181 ttatcggtgt tttttgaaac aaaaaccatg gaaataaatt agttacgcat acccatgtag
   147241 aaactacgcc aacatagatg attttttctt ttttctccat ttctttcagt atgaataaag
   147301 gaaaaatcaa cgcaaccaat cctaataacc ccatagtgta tgttgtaata tcgttattta
   147361 acgcttgaat gatactatct gaaatagaaa gtgcttctag ctgcaaatct gttttgaagg
   147421 gactgtgtat ttcttgataa ttcatctgct gcaacatagg ataccaaaaa tagctagtta
   147481 atgcacaagt caaaaaaaca gcctttacta aattcacgac cacaaatttt atatcgtttt
   147541 ttatttgctt gcttaaaaac aaacgaacta taatcataca agcaatcaat gcactacaca
   147601 atagtgtagt taagacatga gtatacacaa gtagacttat actgacagat aaagcgatga
   147661 cttttttttc tctattgaat atcaatccat aagcataata taaaacagct gggataatcg
   147721 taagagcgat tgcttctgca attgcggatc gataatagat atcaactgtt cgataagttg
   147781 caaaagtata taaacaacta aataaaaaag cagcatctat ctttttaaaa aacttttctc
   147841 cataataaaa actaatcaga atcgtacaaa tatttaataa ccaaatatat agaacataac
   147901 ttaatattaa gttatttgaa attccataaa aaacgactgc aggaattatt gtaagaaaag
   147961 gataaaaata gttcagtcca tctccataat tgttaaatgt tgtatagttg attggccctt
   148021 caaaaataga agataaccct ttgattcgtg aaagatgaaa agataaatca cctgttgtat
   148081 aaatttcttg agcatttctc ataaaattta ctgaatataa agtactaagt aagataagaa
   148141 ctactaaaag aaatgctttt ttacttattt tcatagattt gttgttctcc ttttttaatt
   148201 ctattttcag tatactagaa ttgtcttttt ttaataagtt ttgcgttaaa tttttcatag
   148261 cactagtatg atatactaga aaagaatcaa tttttattac ttttaaatag gagaaaaaaa
   148321 tgaaaaaaaa caaaaaccaa ataatagata tcatttttat gttatttcta agttttgctt
   148381 acgtcatccc tctattcatg tctaagggaa tctatcatag tgtcaatcag gatacttact
   148441 ttcatttatc tcgaatcata ggactagata atgtatggag ttctccagta aacttcaata
   148501 acttcgatca tcatggaaca atgatgaata ttttttatcc atggcttact ctttatccag
   148561 cttttttatt ttataaaatg atgggaaatc ttgttttaag ttataatatc tactattttt
   148621 ttatcacttt tttaacaatg gtcgtttctt atttttctat gaaacaaata aaaaacaatc
   148681 gttatatttc gctgttattt tcgattattt atacattttc cgcctatcgt gccattgaca
   148741 tattccatcg tgcttcattg ggagaagctg tcgctttaac atttttgcct ttgatactaa
   148801 tggggtgtta cgaaatttat attagagatt accaaaaatg gtactggttg tctattggca
   148861 tgacgctagt agtgtatact catttacttt ctgtcgctat ggtttctgta tttattggag
   148921 gaacgttgtt tctttctttt tatttttggg accaaaaaat cgctagactc ttaagtcttt
   148981 taaaagctac cgtcctcacc ttttttttaa gtgcgggatt cttaattcct tttattcaac
   149041 aatctagagc acaagaattg aaagtcccac tgggaaaaga attaagtggt atggctccta
   149101 gtgatatgct gacacatatt ttaaataata actataacaa ctatactatt ggattattct
   149161 tatttctcgg attgattggt gcttttattt ttataaaaaa attaactgct gatgatttat
   149221 ttattttctt tcttggtgta tttgttcttt tttgttcaac caatttattt ccttggcaac
   149281 tttttaacca tacaccagtt aagagtctac aatttgtctg gcggttaaat ggtttttctt
   149341 ctttatttat agcctatacg atgagtatag tgatctatta tattttttct actaaaaata
   149401 attcatggaa aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa
   149461 gcaattctat ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg
   149521 ctatagctga aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg
   149581 atatgattaa taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt
   149641 ttatgagtaa caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga
   149701 cgacacctat ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa
   149761 caaaattatc taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg
   149821 ttattactta tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt
   149881 tgattttatt tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag
   149941 tacaacattc tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg
   150001 ctaaaaagtc tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt
   150061 tatactgtga aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca
   150121 tccaaaaaca gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca
   150181 aatatttcat cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc
   150241 atgataatcc gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt
   150301 tctccctttc gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt
   150361 ttcaatgcag aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc
   150421 agcttgtcca tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa
   150481 ggaatcacgt tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa
   150541 tggaagcgct caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga
   150601 ttcataaagt cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact
   150661 tgtcctcgcg catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca
   150721 tttgtcatct gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca
   150781 agtctttgcc atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc
   150841 atattttagt ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag
   150901 cacaaaatat agctccttgc ttctctaaga cagaagctat acaagaaaac tgtggaaata
   150961 tatatttcag aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg
   151021 acggctcaag ttttactgtt tgagatgcaa gatactcaac ctggcaatgg gcacataatt
   151081 aaagaaaaaa ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat
   151141 agaaggaatt cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc
   151201 aaggtataag tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac
   151261 tggaaacaat aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga
   151321 gacattccac gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc
   151381 tataccatag aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtactaac
   151441 aaatgcagca tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca
   151501 aataagaaac gcttttctta caggactgga aacctctcca aatacactga tcacgctctt
   151561 catttgatct aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag
   151621 cacgcctaat aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca
   151681 ataacaatta cctatgcttt taattgttat tataaacgac tttatcttga tttaacagaa
   151741 acctctattt acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac
   151801 taaatatcta acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt
   151861 tccccacttc ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac
   151921 gaatttaagg cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg
   151981 atttgcatcg cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac
   152041 agtgaaccgc ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa
   152101 aggactttca aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc
   152161 aaggaaatgg taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg
   152221 gaataagact cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt
   152281 gcggcagaac ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc
   152341 aatggatagg gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg
   152401 acaaacagca tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagcg
   152461 attccagtcg tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca
   152521 ataatcactt gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc
   152581 gattctgaac gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg
   152641 taattcgcat caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat
   152701 aaaacacttt ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga
   152761 acagtaaaaa ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa
   152821 tataagatca aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac
   152881 gccatcgtca agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac
   152941 gttcccaata ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact
   153001 aaaataccaa caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga
   153061 ccaccctttc acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata
   153121 attttctaaa aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg
   153181 tctacacgtt gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag
   153241 gaaacgtttt cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat
   153301 aaagaaggag agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca
   153361 gcgtatattg atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta
   153421 catctataca tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat
   153481 gggttaccaa tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc
   153541 ctttatattc ctaatagcca cactagtttg caatatcttt ttttacgtac aagctggcag
   153601 cttgaatatt cgtgattatt ggcttatcct aacacctatc agtcagaatt actttactta
   153661 tgctgtttct tgtactttgc ttttttgtag tattccttat acagttggat ttttaaataa
   153721 attatcgaaa acaacaatta aatatctact attattttta acaattggat tagttggctg
   153781 ctctactcta tttaataaag acccgtttgc attacagaat ggacagagta ttttatggat
   153841 tttttattta tttttaatgg gttatggttt gaaagaatgg aactggaaag aaaaggtccg
   153901 ctataagctt cctcaagtat ttatcagctt gattgtttta ttcggtttga ttattttgat
   153961 gacacaaatt tcactcatca tcagaggaaa tacagataca gcgctgaggt tttctaaacc
   154021 atattctctc ttcgcaatgt attatacttt ctcttcgttt ttatttttag aaatgctatc
   154081 tgataaatta ggtataaaag gaagaggaaa ttttcttgct gtatttttga ttgtcactca
   154141 agttatatgt aatttccctt tagtttctta tcgcgtatct acatttatga aacgtgaatt
   154201 tccaaactca gggaaggcat ggctgataaa tatcgggcag ttttttattt gatatttatt
   154261 agcagtgtcc ctcctagtta tctttttatt ttatatacaa aaaatccgtt tcattcatcg
   154321 tttcatcgaa tacttcgctt ttgattcgat tgaacaatta actcagcgtt ggaaaaaaag
   154381 aattcattgg ctttttgttc ataaaagtta ttttttagtt gcagcatttt tttactgctt
   154441 taccttcgtg cagattttcg ttttagaacc aaaagaaggt tggaaagaaa ctatccaggt
   154501 tgccttaaaa atatttactc agcgacaagc acccatgatc ctgacaatca tcattattat
   154561 gggatttttc ttcttactat tgttgatttc caatcgtttt tggtacgcct tgactgctac
   154621 cttgatcatt aacttactgc tcactatttc gacagtaatc aaaatggaaa tgcgggaaga
   154681 gcctgtcttt ccttcagatt taaaaatgct gaccggattg tcagaactat tatcgatggt
   154741 tagcccagtc ttattaatcg tgggcggatt gatccttctc ctattattga ttactagtat
   154801 tattgtacaa cgtagactac aaaaacagta ttccttgaag attcattgga aacgtcgatt
   154861 tatcagtatc gctgtattat taggatgttt ttcgggtgtc tttttcatta atcacaaaaa
   154921 ttcgccttct ttcttgctgt ttaatttatt taaagtaaac aagactttct ttaaccagca
   154981 aggcgccgta aaagataacg gaccagtcat tcaattccta aacaatatcg acattaaaat
   155041 catggaagaa cctgctggct attcgaaaga aaaaattaat ggaataatga aaaagtacaa
   155101 cacaaaggca gatgaaatca accaaacaag aagtgactgg ttaaacaatc agacgatcgt
   155161 tctcaattta agtgaaagct tcagtgatcc tgcacgtgtc cctaatctga cagtacctac
   155221 agaccctatt ccttacatca aaagcattat aacagaagga acaggaggat tgatgttatc
   155281 cgtcggttat ggtggcggaa cagctaacat ggaatgggaa ggactgacag gcttgagtat
   155341 cagtaacctg tctgcttcgt tagttacacc ttatacccag ctagtggagc gtcagaaaat
   155401 aagtccaaat attaccgatc tattcgatga aaaaatagcc atccatcctt ttacagctgc
   155461 tctttatcgc agaaaacaag tgtttgataa atttggtttt gacaggttct attatttaga
   155521 cagtccggat aaattaactt atacggataa aatagaacaa agcccacgca tatcggatga
   155581 ttcagcatac aaagaaacct tgaaagcttt acaaagcaat acggaaacgt cacaattcat
   155641 ccaattatcc actatgcaaa accatatgcc ttatgatgat tactatagtg aattgaacta
   155701 cactgctgaa ggaactgctg tcattcctaa tagaagaaaa gaattacaaa cttatatgca
   155761 aggccttcac tataccgata cagcagtaaa aaaattcatc caagaaatcg acaaaatcga
   155821 taaaccgatt acctttgttt tttatggtga ccatttaccc gcaatctata gtgggaatga
   155881 ttcgaaaaaa tatggattag aacagcacca gacagattat ttcatttata acaacaagta
   155941 tagtcgtgaa caagcagcta cgttaaataa agaagttgtt gctcctaata actttggtgc
   156001 tatggctctt gagcatgcga atatcaaagt aactccttac tatgcgttgt tgacagaagt
   156061 atctaatcat ctacctgcat taacaattga tcctacagaa agcttgtcta atcgttttaa
   156121 tggaaaacag gtctttgtta cagaggatag tcaaattatc caagaaaaaa atttaactga
   156181 gaaacaaaaa gaaattctag aagattatcg tctgatacaa tatgatcttg ttgcaggtaa
   156241 ccagtacagt gcaacatggg cgacacaaaa aataaaagac taacaaataa actgagcaag
   156301 gggttgtgat aaaagacatg tcacaacccc ttgtttatct cattaatttg cttgtccata
   156361 aacgatttga ttaaagccat ttttcaattc attattcgct gtctcgttga agtaaagata
   156421 attatcggga ttcccctgat tttgttgtgt caccaatact tttggctgat tttgctgaaa
   156481 cccgaataga tagacgtgct ttttcagata tggttctgtc tctgcatcgg aatagacggc
   156541 tgtgatttga tagtctgctt gacctgtccc actttctgac cattctgcag aaacaggcac
   156601 tccaccaata gccatcttcc actccccgtt caaaatagct tgagggactt taagaccata
   156661 taagtccaca ctcatttgat tcgtataact tttgtattgt tgtcccattg tttggcccca
   156721 tttggtcata aatgcctcta attgtgcgga tttgtctgca ttccacagtg tttgttcaac
   156781 ttgttcgctg ctttctgaag aggaagatga agcctctaca gaagaacttg aagtagatgt
   156841 ttgagttgtt tcagtcgttt tggaagactc tgacgaagta gaggtatctg ctgtttctgt
   156901 acttgctgca gttgacttgc tctctgtgct agttgttgta gtagttgcaa ctgttgcttt
   156961 cgctgtttct tttgttgatt cttttttcga ttcattcccg ttggtacaac cagctaacaa
   157021 aaaagctaga ccaattgtta ctaaaatcct ttttttcaat taaaaaactc ctttcgatta
   157081 ctcccacttc tttattctat caaataagca atgtgatttt attgattttt cgtattttct
   157141 catcaatctt tcaaatcttc taaaaactga gaagcacccg ctgatgttgt ctgtaataca
   157201 tcagcgggtg cttcttttat tttaatcttc gtgattttcc aatttacctc acgctttata
   157261 tttgtatttg aagaaacata gacttttagt catcatcgtc ttcctcatcc aaatcttgtc
   157321 ggacagtttt tccttggagt ctttgtactt ggtagatgat ttctttattg aaacttaaaa
   157381 gttcaacaag caattgttgc aagccatatt tttcttcttt ttcagctaat ttgattgccc
   157441 gtgtgataaa gacattttgg aaatcaaaat cttttgcaaa gttttccaac attgttgagg
   157501 ctggtaaata tttgttttga ccatgttcgt aaatttttcc ataacgcatg acttcttcta
   157561 aagtagacgg aacgatttca ttttcaatca ctaactcatg agagatttta ttgaaccatc
   157621 tcgcttcttg atcagcatat tctgaaaaaa gggtacttaa ttcttgcgaa gatgtaccca
   157681 aaacatacaa ttgtgcttgg atcaatttca tttcttctaa tttaaaattt gctaatacat
   157741 gccccaccat ggcgccagca gttggtttat gatggtcgat atctgcttgt tttgtttctt
   157801 gaacaaattt ttcttccact atattcattg atttacgcct cttttacttt tgctgattgg
   157861 acttcataac ctaatttttc cacaactgca gctaattgtt cagggtggac ttcatctgga
   157921 tcaaagtttg ctttgatttt gctcgcatta aacaaaactt tcgtattgct taccccaggc
   157981 tgttgagaaa ccgcatgttc gattttttgc atacagcttg gacaagctag tgtatctaat
   158041 tgcataataa tttttttcat cgtagttctt ccttcctttt tcttgatagt cttattctag
   158101 attacagctt ccataaaaaa attgattctt atcaagtttt acgatttttc ttctttgctt
   158161 tcgtgtccaa aatacaataa acgcatcgcg ttaaagataa cgatcaagat actagcttcg
   158221 tgaacgaaca tcccactagc catgtaaata aacccggcaa ataagccgtt gagtaaaaat
   158281 aagacgacac caatggcaat cactacattt tcacgggtat ttaaaacagt tttcttagcc
   158341 aagcggtagg catgaaccaa cgattcaaaa ttagattgca tcaagacaac gtcactcgtt
   158401 tcgatagcaa catccgttcc tgatcccata gcaatcccga tatcagccgc agccaaagag
   158461 ggagaatcat tgattccatc tccaataaaa gcaacgcgca atccttgttc ttggtatttt
   158521 ttcacaaatt gaaccttttc gtctggtaat aattcagcat ggacttcatc aatgcctaac
   158581 atatcagcta cataatcagc agtcatctga ttatcacctg tcagcatcac taactgtttt
   158641 gctcctgctt gtttcaactt agctaattgt tcagcgactt ctggtcgaat cacatctgac
   158701 acacctatga tttgagtgat ttttttgtcg attgctacaa taatgaccga gctgcctgtt
   158761 tgttgtaatt gatgtaaatc ttgtacttgc tttggtgtga gagaaagttt gtgagcggca
   158821 atcaatttgg tgttgcccgc acaaactgta tgaccatcaa tctcagctgt gattccttgt
   158881 cccttgattg tctgattatc tttgactgct aattgttgaa aatcaatgtc tttctcctga
   158941 gcataatgga tcacggcgcg ccccagagga tgatcggata acgtttctaa tcgagccact
   159001 aaagctaagc tgtccccttc atcctctgta tagttcttga tagtggatac agcagtagtt
   159061 cctttggtca atgtaccagt tttatcaaag acaaaagtat cgattttgga aaaagtatcc
   159121 attgcttcac cgcctttgac taagatgccc cgtttcgctc cattaccgat tcctgctaca
   159181 ttcgaaacag gcgcaccaat gactaaagcg ccaggacaac caagaaccaa aacagtgatt
   159241 gctaaacgaa aatcacgact aatcagacca acaaccaacg caataagtaa aacggttgga
   159301 gtataatact gagaaaatcg atcaatgaat ttttccgcat gggatttggt atcttgtgct
   159361 tcttctacta attcgataat tttggcaaag gtcgtatcat ctccgacctt agttgcttca
   159421 atcgtcacgt agccatctgc taaaatcgtt ccagagaaga cttcatctcc actgattttg
   159481 acgacttgtc gtgattcacc agtcactgcg gcttcatccg catacccttt cccgtcaatg
   159541 actcgtccgt ccacaggaat agctccacca gtttttacta agacgatatc gccttcttcc
   159601 acgtcatcga tttccacttc ttcagtcgtt ccatcttctt ggacaacact agcagtagat
   159661 ggggccatct cggtcaaagc cttgattgac tcccgcgttt tggtcaatgt tttttgttcc
   159721 agatagctac caaacaaaaa caagaatgta acgatagctg actccttgta ttctccgatc
   159781 ataaaggcac caagcacagc aatagtaacg agtaaatcaa ttgagataac tttgactttc
   159841 actgcctgat acgcatggat cataataggt atgaccgcaa taatagaagc tatggctaga
   159901 gcaatgttat agaacaagac aaaaccagta aattttccga taaaaccaaa aataatcaac
   159961 acagctgata aggcggtgat atgatttcga tgctcttgga tccacttttg taatttcatc
   160021 tttctccctc ttttcctttc attcttacga gactaagtat agatacttat ccatcataaa
   160081 aaattgacca ctatcaagtt tagtcaaaaa ggcggttttc ttttggtaga atacactaca
   160141 aaaaatagtt agcaagaaga ctcactaaaa gtgatcttgc caactattgt cgtttctatc
   160201 gaaattttca ttgaatgatt ttcctgtaca gcatacatcg gagatctatt tctttatcta
   160261 agatttttat cttgctaatt ttttccttta gtctttcaaa tccacgtcgt tcatagaatt
   160321 gatacgtaca gccactatct gtaaaaagga atatttcttt tcctttctct cttttttcaa
   160381 attctgttag caacttggta ccgatgcctt ttgttttcaa atcaggattt gcagctaaaa
   160441 aaatgatctc tccatctggc gaatgcttct ttaaatatgc tgcaagcatt tgttcatttg
   160501 ctgtctcgta agctccttta ctttctttag caaatatatt ttgtaaaaaa tcgaatatct
   160561 taacatatat ttgctgtcca aaagaacgat gtttcttttt ttctcctttt atctccgcca
   160621 gcaaaacacc tgccaactcg tcctctgcat acaaagcaag aatttgagtt gctcgattca
   160681 actccatata caaaaaatat cttccgtaaa gattcagcag aagttcattt tccatatatc
   160741 tctcaaagtt cattccttca acagcaaaat agatagcttt tttgtaatct ttcttttcta
   160801 agggtttaaa agttagtttc atcatttcgc ctcctcaact tcaatgattg tgttatactt
   160861 acattataat agacacagtg tctactatct ataatcaatc atctataagt ttgtcgatta
   160921 agcgacagct ttgctttgat atgtcggata aggagatagg taaatgaaaa agacagatag
   160981 acgcgtcaaa aaaacagaaa aagctttggc tgaaacacta tccacattat tagtaaataa
   161041 aaagattcaa gcaatcacta tacgcgaact aacagaaacg gcagatgtac atcgttcgac
   161101 tttttataca cattataagg atatctatga tctatatgat caattagaaa gcaacttttt
   161161 tcgggatcta aatgaaattc tctcttatga tccagcccat agctatgagg aactatacac
   161221 tcgcttgatt gactatgttt acgcaaatac ttttttatat cgcttattta tcctcaacca
   161281 tggtgatatt tcgtttcaga agaaagttaa acaagttatt gaagaaaact atctgaacat
   161341 atggctatat gaagacaaga aaacgattat cacagaagaa atgagattct ttactacgta
   161401 ccacgtccaa ggttgtctat ccgttctcaa tcggtgggta gaaagcaatt acaagtactc
   161461 caaaaaagaa ttattagaac tcttacagaa actgaatgat cacatagaga aaatcatgcc
   161521 ttgaataaaa acatactaag cttagttaag aagaaccgta accttctcgt ataacaactg
   161581 aatcttattt agccaaattg tttactgaag attttttgag taaaatttct ataattccta
   161641 cttattgaaa agtagtttgt agtgctgctt ctccctctct aagtgatata tcttgttgta
   161701 ccaatcaaat tcacctcatc attaataaat tacttttagg ctaatttatt acaacttata
   161761 ggttgaagct gtaaccaatt tatagtcata ttatattttt atcatccagt ttattgaatt
   161821 cttctcgttt acctcaattc attttcaagg ggaatcagct catcatctaa cattttacgc
   161881 catgctcgct tcatttcttg tgggtattta aatggtttcg cttctggatg tttggcttta
   161941 tatccttcgt aatccttact gttgataatt gctttcatat taggaattgg atcttcacct
   162001 ggcatatatt gcagattaga agcatgtaat tttttctcag aaacaaacca ctcattggaa
   162061 aagacatgaa tcaaatcatc acttacttgt gtaaaatagc gacgtttaat gacaaagata
   162121 tcttcattgc tatctagtgt accttcattt ctttgtgctt aaattcaaag ataattttct
   162181 caataaagat ttttgattcc agtaagattc ctttactcct tttacaacat agcgttgtcc
   162241 tttgttttga aacatcttct tcccctcttt gccattaaaa atagcccaat tctctataaa
   162301 gaagattaag cttcattatt ataatataaa gtttaaattt ggtttattac ttactttttc
   162361 ttattctcta gtaagctaat attgaaaaaa atgtaatcag atcattattt ttatatgttg
   162421 taacttcctg ataagaaaac taattaaaag aaagaggtaa ctacataaac taattaaaaa
   162481 agctaacttc atagttggag gataaatgga agaaacttca atgattgttt ctccttgtgt
   162541 tccttcaatc gacactagtc ctattatcaa tctctctttt ttgtccattt acttttgcga
   162601 ctgttcccca ataatcaaat acaggaagcg ttattttttg tcctttagat tcatgtactg
   162661 tatagactat tttcgaatcc atcacttgat aaggcatagt atgccattcc ttgccaattc
   162721 ttacttcatg attctgcaaa tgttctttgt attgtaatgc ttccgtgggt gcatagtcat
   162781 aaagtcctcc gggaaggagt gtcgtgtaac gaataagatt ttcttgattc attaccaagt
   162841 gatctctctg agtgacaact ttatgttcat agtttgcttt tgttgcagca gatacagcca
   162901 acagtaataa aacaattgcc gttatattaa ctacttttct ttgcttagct gatgtgctct
   162961 tcatgaaaac gaacgataaa atcaacgagc caaacagtac ttgaaagcct aagattcgcc
   163021 aaggaaattg aatcatttga atgggtgtat tttgtaataa agaccatgga aaaaatttgg
   163081 tggataagag ccaagagact accgcactaa ggtaaatcac tttttctttg cgattaaatc
   163141 ctttaaaaaa gaagagtgga cagatcaaag aaataatacc aatgattcct accgtaaagg
   163201 ttgtcaggtc attattcatc gcaccaatca aactatccga gacatttaat gcttggtgtt
   163261 gtaaggtcgt tttatctggc tcattaatcg attgaaccat gatttgtcgg aacatcggat
   163321 accaaaagta tgctgttaaa agaatcgtca ttcctactgc ctttgacaac tccacaatca
   163381 accggatgct ttcttttttt cttgtttcac ttactacaaa gccgcctaag actagtaagc
   163441 caacggctat tacactcata aaagtggaaa gaacatgtgt ataaatgagt aagctcatac
   163501 tgactgctaa tggaattgca catggctttt tctcataaat cagtgcgtaa gcataataca
   163561 taactatcgg tataaccgta agtgcaatcg cttcagcaat agcagaacga tgataaatat
   163621 caatcgtacg gtacccataa aatgtatata aacagctaaa tataaaagcc gcatcgattc
   163681 tttttaaaaa ttttagtcca taatgaaaca tcagcatgat agtacaaaca ttcaatagcc
   163741 aaacatataa gatataacta acgattaaat tattacttat ccaataaaaa atgactgccg
   163801 gaaaaaaggt caagtaggga taaaaataat tcactccgct cccataatga ttaaacgtcg
   163861 taaaattgat tggaccagaa aaaattgaag ataacccttt gactctagca aaatggaagg
   163921 caatatcccc cgatgtagaa atagtatgaa catttctaaa ataattgatt gaataaagtc
   163981 cagcaataat tgctaggaga atggacaata ttattttttt ctttacttgc attacttaat
   164041 tatatactcc tttcatggat tgctttatct agtataactt aaattttaaa taaatcgtta
   164101 tttattttta ctctcactta aaaaagcact aaaattataa gagagggagg taatggtctg
   164161 aacaccaaga attgttccaa catcgaattg cttaactaat gagttcaaag cttaattatg
   164221 tggctcttat atttaccgag cgtggattgc gatctcttta gcgaaacaaa catctttcat
   164281 aaaaaaaaca gccatatccg ctttcttgga tatgactatt tactactact ttactatctt
   164341 tcattcttct tgtctcactt atgataaggc gctgcgtatc atgagtgaat gatattagga
   164401 aaacttcata aaataaatct tttatacatt tccacttcta gatggtcatt catgttagaa
   164461 gaatcaaact atttttttac tgtcgtaata atattaaaac actagaacaa atcaattaaa
   164521 ttccataaaa tataaataat agacctagca tcatctggta taaaatggat gctaggtccg
   164581 ttatatttac tcaaaattga ttctacgaat agatcttctt atcatttcta ttttagtagt
   164641 catgcgttgt tgttcttcta aaggtggtaa tggtataagt agtttagata aatttgtacc
   164701 atttacattt ggttgcccag ttccgtaaga aattttttct aataacttcc aatataatgg
   164761 cgagtctaag aagcagtcta cgtattctga taataatttc tcaactaacc taattcgaat
   164821 caagtaagaa gcaaaaacag attcctcact tatttcctta accaagaaac tttttcccat
   164881 agttcctcct gtccttgcta tcaaaatgtc attttcttcc aatcttaaat caactagttt
   164941 agaattacta atatcacagt aaggcacact ggaccaattt acacgtccct cttgtatatc
   165001 tgtgattctc aaaaacttaa cattgccttg ttttttcgcc gaatctgtat agccatattg
   165061 aatcttagta gaaatacttc ctaaataaac ccatagtcga acatcgctag atgatttttc
   165121 ttgtcgttct ttatgaacaa cagtgatcat cttgagttgt ccgactattt tttagttgca
   165181 gcaatctttt tgggacgctt atttgcatga agcaacgttg ctaagtctag taataaaaca
   165241 ttggctaaca caggattggc tttctcgcga tacgctgcgg cgccactttc aaacaatact
   165301 gctagacgat cttctacaac aacgatttgt tccaaatacg gtggaaattt gattcgtttt
   165361 aggatccggc taggagagtc ccgctcggac actaagagtt ccgaggtata aggaccataa
   165421 gaacgactgg ctaagagata atggccataa aaagccacac cttgaatctt atcagatgtt
   165481 tttctgctca ttgtcacggt tggttggatt ttgtctttgc ggcgaattgc tgttttcatt
   165541 tcagctatcg tcggcaatag atagaaagtc aactgtcctt caccttttag tgtgaagttg
   165601 ccagatacca gttgatcatt gttttgagtc aaatacgatg cttgtggaat ctctgagaga
   165661 tcagttgtca gccaataagc gattggctgt cccgtctctt cagatgtatc tgctaagata
   165721 tcgatcaaac gtaaagcggc tactcgtcca gtagcagatc ctgtgattgt caaccataaa
   165781 agttcccgtt ttgtatcata tactaagccc cctacatgtg gccgtttagg taaaatcagc
   165841 agtttgatcg gctgtcctgt ttctctatcc agtacatgaa taatagaatg atgctgatgg
   165901 tcatagcagt aggcagaaat aaacacaaaa tcttgagaaa aagccaaccc ttgcggcgtc
   165961 atccattgac aaacctcatt ttcgccggtt tccggcacca ccattcttgt ttctgttaat
   166021 ccaggaatgt tatattgttt tgcttcagcc ttttcccaag ctgcttgaac aataggatac
   166081 cgttttaact ttttttcaat atcctgatgt atccgttgct gcttccaatg cttaaacttt
   166141 aggacatagt aaacaagaag gccaatgacc agcaacacta aaatcattat tgctacccgc
   166201 tgtgtcattt ttttgcctcc ttgttctatt cgatcaatcg tccatatacc gctattaatt
   166261 accgtaacta catgtttctt ttactagcgt ttctttatag gatctttcat aatctcattt
   166321 atagtaacat taagaatccc ttattctact atagtcactt ccacatcaat attgcctctt
   166381 gttgctttag agtatgggca aaatgcgtgg gcttcttctg cgattttctc tgcttggtcc
   166441 ttctcaagtc ctggcaaaga aacttctaaa acgaccccga tttggtagcc cccatcttct
   166501 aattgaaata aagatacccg tgctttgact tcagaatcag actcaagatt attttcttcc
   166561 agcatgtgtt gaaccgcgcc gttgaagcaa gatgcgtatc cagccgcaaa aagttgttcc
   166621 ggatttgtcc cttcactatg gattcctggg cggtcgatct tcatgtgcat acttccgttt
   166681 ggtgcctcaa cttcgccatc acggcctcca cgattgatca ttgctgtttc gtacattttt
   166741 ttcattgaga tcatcctttc tttcgtcgtt ctatattaag tgtaacgagt attttttaat
   166801 ctgacaaagg taatgcttgc attcggcgac atcagttaga caacatcgat gatcagtgat
   166861 agaagcagtt tatctataca aaaaaagaag ggttctatga tatttggtgt ccttactcgc
   166921 cttgagtaac aacaccaaat atcatagaac cacttaatca gcttcacact gctttttcta
   166981 ctttcgtagt tgaaaaaaac ttaaacaaat catcggcaaa tagttaaaga aaaaacttaa
   167041 tcgttgaaag aagcccaatt gcttaaagaa ggcaaactga ctaatcgacg gaatccgtgc
   167101 taatccgtaa agacctgcaa ttagtaaact aataactgtt aaaacaagat aaaaatggct
   167161 aagcgtacct tgaccacttt gacgataaag cagaacaagt aacaatggga aaagtagaaa
   167221 gcctgcatag cctagcccag aacctatatt atggatccaa gtcgatagat tacaagagct
   167281 ttcattcgta ttgatactga ataagccagt aaaaatacaa tcacctatcc catataaacc
   167341 aactgttgca acaacaagta ttgcaagtgt ttttgatgtt ccaacaaata ggtgatacaa
   167401 cgcaggcaac gaaagaacaa aaaaaagtcc agatacaact gaccagacca agaaagcacg
   167461 tctgactgga ctatctacgt caccaaacac actaattacg gtagtcattt gattcagttt
   167521 gggataaaaa aggccaagga tatacggtgt cgataaatca ctcatcacac ctaataataa
   167581 aaaataaaaa ccatattttt gcaaaaaaat cattttcttc ccctactttt tagtgattat
   167641 tcgatacatc caacaatcaa attttttctc attttaacac atatttttat tctagttgtc
   167701 ttttgaacaa tcaataacat aaaggtgagt ttcttctaca ttttaaaaaa tcgaatggca
   167761 ctgaagagtt cagttgtcag catcaatctc ctactcaaaa aggcaaaaaa acgtaagtca
   167821 gatgttgttg acatctcact tacgcatcca ttgattgaaa atttcttagt aaaatctact
   167881 agcctaaaaa tttgaagact tcttctcgat catacagttt aaatactttc tcgcataaaa
   167941 attgagtaat tagtgccgca aaaacaggga tgactaacca acaaagaaca acaagaaagc
   168001 catttagccc tgcatctaaa gaagccaacg gaccaacaag tccgaccacg ccgaaaccag
   168061 cagaagcagg cgtacctgaa atagaaaata gagcgacagg aatagctgta atcactgcac
   168121 taactaaaca aggaataaga atcacaggtt ttctaaatag attaggcatc atcatcttca
   168181 tagcgcctaa agcaatcgca attgtcacac ctgacttatt aaccttccaa gagttcacta
   168241 ctaaaacaaa agtggttgca gctacgccca tcgcagccgc accagcagaa acaccatcta
   168301 gttggattgc cattccgatt gctactgttg aaataggaga gataatcaaa aaggcaaagg
   168361 aacaaccaat caagatagac attaacagcg gttgtaaggt agtgaagtta ttaatcaatg
   168421 atccaattgc agaagtaatt gctgttacaa aagggaataa ataaaaacca agtaaacctg
   168481 cacctacacc tacaacaatc ggtgttaaca caatcgacac tgacccaaaa ctatttccaa
   168541 tcagtaaaat cagcccgaca gcaatcgatg ctgtcagcat cgtattaata atatcgcctg
   168601 taccagcacc aacgtaaaca cccgtttcac ccaaatcagg tgtgaatttg actacaccag
   168661 aaccaacaaa agaagctccg gctacaacca tcattctcat cggatcaaat ccaaattgta
   168721 aggcaatcaa accaccaatg atcaaaggtg ttgctagttg aaaaataact gctaactgtg
   168781 cgatcgttac cgcaaaggga tacgcactaa aatattttaa aatggcacct aatactgcat
   168841 taggaatcaa tccaataatg attcctgttg ctgtaccagc gaggacttta ttcagaaaaa
   168901 ttttcggtga tattttttgt gaagtttcca ttcaatctct cctttatttt acgttcaaac
   168961 tatcttcttt tgcatggaca agctcagtca agaaatcaca ataaggcgtc gcaacaccat
   169021 atttcttccc ttttctagaa attgcgccat tgatataatc gatttctgtt aatcgatggt
   169081 tcttaatcaa gtcttgatac attgaaggat aatgtaaccc aattgtttcc gggtcaaaac
   169141 aagattcaca atgtgcaatg acttctggga catctagttc aatcttctct actgctgcta
   169201 ctttggcaaa ctcattgaca atcgtcgcca ccattttatg agcagtcgat gtttttccta
   169261 gctcagccat attaacgtct aagattgtgc atagtccgtt cattgttccg ttcacacatg
   169321 ctttgcgata aatagaatag tgaatattgt cagaaaaatg agcatttaat cctgattcag
   169381 acaatttgtc tgctaatttt tttgcagctg cttcttttcc atcacctaaa ttttgtaatt
   169441 ctactgaacc acttccaaat aatttgacct gaccaggacc ttcaagacct gccgtccaca
   169501 tggtatttcc aatatagata ttttccattg gtacaaattt ctcaataata tcttcatgcc
   169561 cgataccatt taatagacat aagacctctg tatctttttt gattaatgat tggatatcct
   169621 gcagcatttt ttccagctgc atcgctttgg taaatagaat aatcagatca acttgatctt
   169681 ctttttctac ttcggattga agaacgattg gtagttttgc ttctacctct ttaccattaa
   169741 aatttgcttg taatccatgt tctttgattt gttggacatg ttctgcccag ccatcgatta
   169801 ataatacttc gttcccactt tggtgaagca tcaacccaaa gcgacttccc attgcgccag
   169861 cgcctgcaat tgctattttc attgattctt cctccttttt ctttctatta tactcatttg
   169921 agaatctttt cacaacctcg ttttgttatt cttctcttat ttatcatgtt tatatccacc
   169981 attcaagtta ttttatcagc aaatcaataa aaactggaat agaatcccaa tttcatcgta
   170041 agagaaacta gactagcaat aagaaaatag aaacattcat agaaaaaatc tcactcctct
   170101 gattataaaa gagggggcat taacctcgtg tatactaaaa ccttccattc attcgatgga
   170161 tgattttctt tttgtgaaaa aatattcatt gtagtacgat gttgcgcaac acagacggat
   170221 ggtattgctt attatttatt aaaatcgaac aatacactta tttaaaaact tctttcccag
   170281 attttcttcc atttttacag ttataactag tattatcatg aaaaatagcc ttttattgtc
   170341 tatgggaata ataatttaaa attggtactt ttttattaat tagttcatcg cccaataatc
   170401 tctccagttt cttagcatct cctgactcaa ccggaattat ccaagcccag tctaaataat
   170461 tttttaaaat ctcatcatat tttgcaaata tttcattgcc atattccgtc cttaaaatta
   170521 tattttcagt gccagacaag tgatttatat tgagttgata aaaaagatct ccatctaaaa
   170581 aaatatcatt agaagaagat ttccccacag taatgaccat tccatcagct ctatgtgtta
   170641 catacaaata attgtatgac ctcatttcac ctaatgaatc aattatttct tgccctttag
   170701 ctctaatata ttgatctcta ttacctatct cactcttatt taacaaaagt tttttcatgt
   170761 caaatttaat cggctgtttt atttttgtaa attcgcttct actactaatc caatccgaga
   170821 ttaatttttc aacatgtccc agagtaaact ctgatttatt cgctaaatac tttttttgtt
   170881 gtattaaaga catctttccc cgaagctcaa aattgagcct acatcgattc tctatctctt
   170941 caaaaagcat cttatcttca tcattcttat attcatcatt cataaaacca atccagctcc
   171001 tcgattaaga taatatttta atctcataca aaaactagct ctcatcaaaa caaccattgt
   171061 tttgatttct catgaaattt ctttatactt ttctccgatt ataaattcct aaccccttga
   171121 gaaaattacg catatattta tctccgcaag gcttaaaatt tcgttggcct tcttttctca
   171181 aaaatgcccc gatttcgcct tttgatggat agatttctgc ctcagcaaaa acgtctaaaa
   171241 tatcatcagt ggtcatagac aaggcgattt ttaatttttt tagcaacaca ttgttgatat
   171301 atttgatttc cttgcttttt tgcggcgtgt ctactatcgg cttatctttt tgcggaccac
   171361 gcgccaagag aatcaggcca tttaggaatg cgtcaaaaac ttggttgttg atggtttttt
   171421 catacacatt attttctgca ttatctttgt tcttatcctg tactttggtc agtaccgcct
   171481 gagcttcttc tttagtcagt ggtacaccgc ctagctcaaa agctttaatc aaatccgtat
   171541 ctttcaaatc aatcgcataa cgcaatcgca ataacctatc attgttattc atttgttcct
   171601 ccgaagtatt tagtctttac tttcttacgt actttttgtc ttgctaagat ttttaggatc
   171661 aatgataaaa accgcatcac ttatgataag gcgctgggta tcatgagtga atgatattgc
   171721 tgaatacaaa tagtccgacc acattaagtt gatcggactt cataagcatt ttattgacta
   171781 atactttttt gcttaagaat tgattctaac atcttttgct aaagttaagc atcctatagt
   171841 tcctgcatta tctgctaact taggtgtgac aatgtattct tcgatatttg gcgtatcgac
   171901 atagccgtta attagttcta aaaaacgtct ttgtactttc tttttcagat gttcttgttg
   171961 catgacacca ccaccaaaaa ttataatatc tggtgaaagc atcagggtcg tattacaggc
   172021 acattgtgca atataatccg cctcgatttc ccagaacgga tcttctatag aaagatcttg
   172081 tcccttggtt cccgtacgca tctcgatagc gggtccagat gccaaccctt cgagacaatc
   172141 atgatgaaaa ggacaagtac cagagaaagt atctcctgaa tgtctcttaa ctaacgtatg
   172201 ccccatttct ggatggctaa acccttcaac aaaaatccca tcttgtatag ctcctccacc
   172261 aattccagtt ccaacagtgt aataaacaac actggataaa ccttttccat gacctgaaac
   172321 atactccccg taacaagcag cattcacatc ggttgtccag gcaattggaa tttcgaaatg
   172381 cttattcaac gttccaacaa aatcaaaatt tttccaggca gtttttggag tagaggtgat
   172441 atgtccatag gtctttgatt cacgatggat atcaatcggt ccaaatgacc ctactccaat
   172501 gctttctaat tgttctttat agtgattaaa gaaatcaatt accaaactca ttgtttcatt
   172561 cggtgtagtt gtaggaaaac tcactctttc taaaaccttt aaatcatcat tgccaatggc
   172621 acaaacaaat ttagtcccac ctgcttctat tgagccatac atttcttact ccaccgtcct
   172681 tttatctagg aattcgttct tccctgtcta atagtttttc gaaaaactca tgtggttcct
   172741 tttggtacca ataggcaaca gaagaaatat cgtcttgacg ctcataaagc ttgatgtcat
   172801 catttcctag ggtttggaaa gtaacacgta aattttcttt gaaactaatg gggtcgggta
   172861 aatgccagcg gtacaaccca tgcatcggta aagcatcatc gccaaaacca tgaactggat
   172921 ttggattagg ttttccagca ctaaaataat cccgggtgtg atctctttta gattgaaaag
   172981 gatagcccat aaatagcgta ttaaaatttt gagcatatgc cctttctcca aagttctttt
   173041 tatggaaagc ccaagcgccg ccaaaataat cttctgctcc tgtagatgtt attgtaggga
   173101 aatcttcatc tccatctata taaaacttaa attctccttc cccccaccag tatctttcca
   173161 atgcgcacaa agcaaagtag gttcctacat aatacccagt gcctttgatt ccatcaataa
   173221 gggtgtaatc ttttcctttt gtcgtgatgg gttcacggtt ccatttagca tgaaaataca
   173281 tcgcatcatc cggtacttca gtcaatgcat aattaaatgt ataaaaaatg tgcttaataa
   173341 actcaggatg ttcattcgtt aaagtaatct tagctttttt tcgaaatggc atctcaaaat
   173401 agcaattcat ccctgatgtt ggattcacta cgattggtaa ggaattcact tcgcatcttg
   173461 tcccaaaccc attacaaaaa aaatctccta atggactttc tacagaagga gtggattcat
   173521 catcccaata aatacgcaaa attaaatcac gtaagacgaa actcccagcg attgttttgt
   173581 ctgtcactgt catccaaaaa tgacgtaaaa ttccagggcc ttccatttct gctaaagtaa
   173641 ttgtctctcc agcatttatc ggaacagaag gtgacccttt tctcccaatt cctagatgac
   173701 tcgctgtcat tgccgctttc cctttttcac catttttatt ttctggtgaa atagttctac
   173761 tttcctctcc cttgtggagc catatattat ttaaacaatt cattttattc gtcctctatc
   173821 ctttcttcac ttttttgatt attcatatag atgatttgtt gttgtgcctc cgcaataatt
   173881 gctccaatat ctaaattatc atttcctaac tgcaaagcga ttgacattgc caagggaaga
   173941 ttcattcctg caattacgtg cactctgtca gaaatatagg taaaaaactt ttgatttaca
   174001 cttcctccag ccatgtcagt taaaacaatt atttcgtctg tagtatccac taactcttta
   174061 atttgttcat ctatatcatc aatttcttcg acatatgcac ataagctact aatttgatta
   174121 tgcatacctg ttaaaaactc tagtgtctct ttaaaaccct cagctagtat tgaatggcta
   174181 gcaatgatca atcttctcat aattcaaatc cttttccttt acgctaatat tccaaatgca
   174241 gccataacca tcgcaaaaac aataacgagt agaatcaaaa tggtcatact gatttttttc
   174301 ttactgagta gataatatgc tgcagcagta ataattactg gaattaatgt tggtaaaatt
   174361 ttgtccaaca ttggttggat tttcatacta acttcaccaa aagtgaattt aagtggcgtt
   174421 gaaacactga taactgaagc aattaatgat ccaactactg ttagccccaa tactgaagca
   174481 gcttcagtta aataattaat cttttcaccg tattcagtaa caatcttcat tccctgctta
   174541 taaccaaatt caaagcctct cattcggaaa aagaagatta ccatgattaa aatgaaccat
   174601 aaaaagattc ctacaggatt attttctaaa gccatataag ccgcaattga tcccatgatg
   174661 gttgggatca tcgtcatcaa aagagagtcc ccaataccag caattggacc catcgtacca
   174721 actttaaagt cattaatcgc atcttttgct tctaatcctt gcttttcctc cattgcaaca
   174781 caagcaccta aaataatggc tgccagccaa ggctgtgtat tgtaatactg aaagtgatta
   174841 tttaaagctt cgatgtattc atcatcatct ttgtataatt ttcttaaaat aggtgacaat
   174901 gcgtaagtca cagatgcacc ttgctgtgtt tgataattaa aggtacaaac tgacatcaac
   174961 caacgatagt tcgctttcct aagatccttt ttcgttactt gatatttttc gctcatttta
   175021 ttcatcctcc ccaataaacc caacatcaga agctcccgca gaagcagtga tttgtgcatc
   175081 tttttcagct ctatgataca cattaaatgc taaagcgaat cctatgatcg ccgctccaat
   175141 aataggtacc ttcaaaaaag cggctaagaa aaatccaaca agtaaataac taatgtatcg
   175201 acctgtaggc ataaagcgta acaacatcgc aataccaaca gcaggcaaca tttttccggc
   175261 taatgttagc ccatccataa accaattagg taaaacatcc aaaacgtaat taacagcaga
   175321 ctgtcctaaa gtaacggcaa taaatactgg aattgctgca gataaaccaa agaaaattgg
   175381 ccccaaccac aaaacactat tcattttctt aaattctcgt ttattcgcat attcctgtga
   175441 tttgcgaaca acaaaaccat ttaagatttt gacaattaca tctagttgca cccctaacat
   175501 acctacaggt agtcctattg ctaatccggc ttctactcca agctggctac caaatgccgt
   175561 tgcaataatt gcgcacgttc catagtccgg tacagatgag ccaccaaaac cggcaactcc
   175621 taatgacatc aattgaactg taccgccaac tatcaaacct gtttttatat cacccatcaa
   175681 aattcctgtg aataatcccc aaaaaactgc gtaatttaca aagatcatcg gaatcccata
   175741 ataatcaaca tgtttaataa atgctaatat agtcagcata ataatttgaa aaatagtaaa
   175801 atgcattatc cttactcctt tatcctaatt ttgattgaat ttcctgtgcc ttatccgccg
   175861 gaacaaattg tgtaacaatt tctacaccag aatccaacaa ttttttatac gtgctgatat
   175921 tttcatctga tgcataggct ctagaagata gctgtaatgc attatcttta acaacagtac
   175981 cgccaacaat tagtcggtca attctaatac cgttatctac taaatgaagg attgtttccg
   176041 gctctttcac gatgacaaat actttttcct gatcgtattt ccccttttta aaattcgcaa
   176101 tcgctgtttc ttcagtgata attgatacag ccattccagc aggtctagct aaacgcatac
   176161 ttgatttttg gatgtcgtta tttgcgacag aatcatcgat caccatcact ctgtttgctt
   176221 tagacattgg tgcccattgc gtgacaataa tcccatgcaa taaacggtta tcaactctcg
   176281 acataacaat actcataaat acttcctcct ttatctttca ttttcttaca agcaattacc
   176341 atgccaaaac aaaaagaact tgataaatca agctcttttt acataatcat aaacgtattt
   176401 aacctcatga atcgggatgt cgacactata ataatgttca acgacactga aagattgctt
   176461 aaaatgtgtc tgaaattttt gaaattcctc actcgtttca ttgaaataaa tttcatcttc
   176521 caaagtattt tgttttacta atcgttcaat taagcaacta atgtgaacat ataatccgaa
   176581 acacgtatta ttatcaaatg aaattcctag atcattttgt aagatgtata tagcatctga
   176641 aacccgttct aataatttag tcggatttaa tatggtcaat tgctccatta aattcgttag
   176701 actaaaattc ttcagaatat tattgttcaa aactttcagt tcattatcat taaagtgtct
   176761 attcaattga ttggagaatt tacttatatt cattcctaaa atcatatctt caattgaaaa
   176821 gaattctaca tcttctagat gtggatttag tgttccaaca ataaaaagaa tattgttctc
   176881 tttacgaagc tccgataaat agttttcatt cactaaagag taatagtcac acgtttctat
   176941 gacaagatca gtatcccttg gaaagctatc ttcaagtatc ttttttaatt taaacgaagt
   177001 tcctaatccg cttgcacaag aacagacaat catatccttc ttaatatttg atgattcata
   177061 aatataatcc acattttgat tttcttttac ttgttcaaga atctgtttaa gcggtaaatc
   177121 taataatatc tgatttgcaa catcaatagc cattcttgtt gaaacattgt ttagtagtcc
   177181 gaattttttt tgagcgtcta gttccaaatt tagataaatt ttctccagtg aacccatatc
   177241 aacaagtaaa ataatttctt catatccggt acactcttgt aagtatctat taattttgtc
   177301 tgcaatctca ctacttgaag tcattagtgg catatcaatg gcgtcgtata tatatctctc
   177361 aacgatccta ttagcagtac ttgctataca ggttgccgtt agatctccat gagccaaaat
   177421 cagtgctaaa cgtttttgct gaaattcttt actacacatt aacttaaata aaataaccaa
   177481 aacatttttt tgaatttttg ttaaataaat atcccctatt tgaataaaac gattgactaa
   177541 ttgaacttct cgactacacc aactattaat atcattgatg aaggaatata atttatcgtc
   177601 cgatgttaat tggatttgat aaatccaaga attcaaaaag atagacgtta tttgaagata
   177661 ataatagtca aattctattt gaaaatctct ctctatttcc ttaacatact gtctaaatcg
   177721 aatctcttga taggtgggat atgttaaagc tgaattattt tggatatcaa cattcttttg
   177781 tctaaaatat ctatcatatt tctcaaaaag ctgctgctta tcagtgtctt tcttccacgt
   177841 ttgataaaga tactctataa gttcgttgat attaaaaact tcgtctactt tttccaaatc
   177901 atccatttga atatatttta attcattctg tcttttcata accttcatgc cgttgaaatt
   177961 cacccattgt ggtaaatgat acatctgaat ataaagttct tccaaatcat ctaagaaata
   178021 aaaggcattg gcacaggtca attgaatgat atttttcaaa ttgccaacat tttcactaaa
   178081 gcgatagttt aataaaaaat ctaataattg ttgagatatc agtattttct ttgctagtct
   178141 ttcttcttct gatttaaaaa atatcttgat taactgtttt ctttcataaa gagaacgact
   178201 gtctaatgtt ggaagtgtta gctttacagg tattcttctc aacaaagttg ataatagttt
   178261 atcctcaggt ggttctgatg ttgcaaatat aatccagcaa cttgatgtgt accagttttc
   178321 attatcaccc atccgatgaa atttcctcgt atccataaac tgaaaaagtt tctcctgaca
   178381 ttcattgctc aatgcatgaa tttcatctaa aaataagact ccattattag ctaattctgc
   178441 taaacccttt cttccatctt cggctcctgt atatgcacct tttacatgcc caaaaaaatt
   178501 ggacaagaaa agttctggat tattggcata ttcactacaa ttaactgaaa tgaaagatgc
   178561 cttctcttct atataattat tggccaacaa atccttatac acataatttg ctaaatagct
   178621 ttttccagtt ccagtctcgc catgaagtaa aatgggtaag ccatttggcg gatagtagat
   178681 tgctgcttta atttgagcaa ttacatttcg cagacttcct tgacttccga taaccctttc
   178741 tattccaacc ctaaaagagc tatcatacca tttttccgct tcctctgaag agctaaaaat
   178801 ttgttgattt ggaactttct ccggtaaaga aaagaagatt actggacgag tattgatttt
   178861 tccaaattct ccatctttta cgaattcatt caaatattga gagacaatat tacgactaac
   178921 atttaatttt tcagatattt tctgtgctgt attgttcccc catttatttt taatctcaga
   178981 gttctctatg acataattga caatgcgttc ttttaattct tccatcacag cacctcctcg
   179041 acactattcg aattttgccg acagtatttt tccataaata tgacttgcta aaaaggcttt
   179101 gtttagatca ttcaattgga acgttgcttg ttttgcttga tccgctaaga cgttgaaaag
   179161 atactctggc gaaatcgcat accccaaaat atcaacgatt gttgcaatca gatcttcttt
   179221 tgattcttca tctgaaagta attcacgata caacatcgtt tgtttgctta ctgtgtcata
   179281 ttcctctaag gattcatctg ctaaaagaac cacttgttcc aacaaacggt cggataaata
   179341 tttatagaaa atcagcccca ataaataatt tttatattct gacgcatcca ttttactacg
   179401 gagattatct gccgcactaa ataacttctg atttaattct gcacccaaaa agatacactc
   179461 ctatctctta tttaaagttg ctatataccc tcttatttta gcatactcag ttttagaaaa
   179521 agcaaaaaaa taaagaaact tattcttgat aacaagctac gtgacgtata tccctaacac
   179581 aaaaatagcc atatcctttg gtttggatac ggctatttga tattactcat tcaattatca
   179641 aatcacagta ggtgtgaatc tcatcgggat cggcaatatc tacttctgat taggatcaaa
   179701 ggtgattttc tcaataaagg tgtgatacaa agctttaact tgcttcttct cactctggat
   179761 aagaaagcaa tccaaactag tcaatatctg ttggacatct ttgacttgaa tcggttcccc
   179821 ttgttgttga aagatcccta agatttcatt ctctcgaatt tgtaactgtc ttcgtttttc
   179881 agtcaattca tttaaacgat cttttaacat cggaaacaat tcgggactat cttccaaagc
   179941 agcttcctgt ttttgctcta atagttagat tttgcgtacc attttcttct tttattttcc
   180001 ttcgattgct gattttaata tagtctaaag gaaatttata tttattgtat tctttataac
   180061 ttcaagagaa cttcgtttga gtattactgt atttttttaa ttccataaga aaattagtta
   180121 ttacctgcca cctaatcagc aaatctgaac ctgagtcaat ataattaata ttattttcca
   180181 tctccaacat tgtaagtact gttggtgtta atatagggac tttgtacaat tgtatttttt
   180241 tgattttatc atctgcacaa aggagatttt ttaaatctaa tccttctttt atttcaaaag
   180301 ttgaattttt taaatctata agagtaaaac caattccttc atactgaatt tctgaaaatc
   180361 gaatttttct cccagttgat agatcaaggg gcagaaaacc ttttattaca ttgtgagtgt
   180421 caccatcatt tacaatgtca ttaggaattt tgattttaga attctgtgga gcacctattg
   180481 gaagataata attcattata attttgtagt cttcatgagg aaataaagta cttttaggat
   180541 tgctaagata ttctcgtacc gaatctaaga atggcttatc cattgaatct tcatccccat
   180601 ttaaactaaa aaacattcct acaattgctt ttgcaactct aagtgaattc attttcttta
   180661 ttgaatgtgt taaatcagac ttaccattct ttaaacttgt attgttataa aggaaatttt
   180721 tattgtcttc tgcaaatcta atatattcct caacatactc actaccaaaa aaactattac
   180781 atttactaca taacgtattg gaatggcaac agtttttttg acaaatttta taaggtgcag
   180841 aacttctttc cgtatgctat tccgattgtc cttgacaatg agcctcctcg gatgtgacga
   180901 tcttcggcag gagctaacag ttaaagttag actgcctcgc tagcgttagc acatccgagc
   180961 tcattatcaa ggattcgctg cgccaat
//
LOCUS       DAFCNJ010000002       102913 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000002 DAFCNJ010000000
VERSION     DAFCNJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 102913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.002"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            651..2261
                     /locus_tag="LNE35_000163"
     CDS             651..2261
                     /locus_tag="LNE35_000163"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBK6909354.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2553..3422
                     /locus_tag="LNE35_000164"
     CDS             2553..3422
                     /locus_tag="LNE35_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBK6909355.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3587..4858
                     /locus_tag="LNE35_000165"
     CDS             3587..4858
                     /locus_tag="LNE35_000165"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK6909356.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4855..6150
                     /gene="rho"
                     /locus_tag="LNE35_000166"
     CDS             4855..6150
                     /gene="rho"
                     /locus_tag="LNE35_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBK6909357.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6249..6512
                     /locus_tag="LNE35_000167"
     CDS             6249..6512
                     /locus_tag="LNE35_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBK6909358.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6957..7406
                     /locus_tag="LNE35_000168"
     CDS             6957..7406
                     /locus_tag="LNE35_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6909359.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7463..8881
                     /locus_tag="LNE35_000169"
     CDS             7463..8881
                     /locus_tag="LNE35_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6909360.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8881..9741
                     /locus_tag="LNE35_000170"
     CDS             8881..9741
                     /locus_tag="LNE35_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBK6909361.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9915..10688
                     /locus_tag="LNE35_000171"
     CDS             9915..10688
                     /locus_tag="LNE35_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6909362.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10835..11200
                     /locus_tag="LNE35_000172"
     CDS             10835..11200
                     /locus_tag="LNE35_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBK6909363.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11348..12556)
                     /locus_tag="LNE35_000173"
     CDS             complement(11348..12556)
                     /locus_tag="LNE35_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6909364.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12575..12853)
                     /locus_tag="LNE35_000174"
     CDS             complement(12575..12853)
                     /locus_tag="LNE35_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909365.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            13007..13702
                     /locus_tag="LNE35_000175"
     CDS             13007..13702
                     /locus_tag="LNE35_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6909366.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(14144..14941)
                     /locus_tag="LNE35_000176"
     CDS             complement(14144..14941)
                     /locus_tag="LNE35_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBK6909367.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14938..16122)
                     /locus_tag="LNE35_000177"
     CDS             complement(14938..16122)
                     /locus_tag="LNE35_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK6909368.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(16147..17394)
                     /locus_tag="LNE35_000178"
     CDS             complement(16147..17394)
                     /locus_tag="LNE35_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK6909369.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17609..19354
                     /gene="dnaX"
                     /locus_tag="LNE35_000179"
     CDS             17609..19354
                     /gene="dnaX"
                     /locus_tag="LNE35_000179"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBK6909370.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19374..19688
                     /locus_tag="LNE35_000180"
     CDS             19374..19688
                     /locus_tag="LNE35_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBK6909371.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19713..20309
                     /gene="recR"
                     /locus_tag="LNE35_000181"
     CDS             19713..20309
                     /gene="recR"
                     /locus_tag="LNE35_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBK6909372.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20373..21017
                     /locus_tag="LNE35_000182"
     CDS             20373..21017
                     /locus_tag="LNE35_000182"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBK6909373.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            21032..21361
                     /locus_tag="LNE35_000183"
     CDS             21032..21361
                     /locus_tag="LNE35_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBK6909374.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21375..22313
                     /gene="holB"
                     /locus_tag="LNE35_000184"
     CDS             21375..22313
                     /gene="holB"
                     /locus_tag="LNE35_000184"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBK6909375.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22349..23173
                     /locus_tag="LNE35_000185"
     CDS             22349..23173
                     /locus_tag="LNE35_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBK6909376.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            23166..23513
                     /locus_tag="LNE35_000186"
     CDS             23166..23513
                     /locus_tag="LNE35_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBK6909377.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23582..24454
                     /gene="rsmI"
                     /locus_tag="LNE35_000187"
     CDS             23582..24454
                     /gene="rsmI"
                     /locus_tag="LNE35_000187"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBK6909378.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24563..25684
                     /gene="dinB"
                     /locus_tag="LNE35_000188"
     CDS             24563..25684
                     /gene="dinB"
                     /locus_tag="LNE35_000188"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBK6909379.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25738..26340)
                     /gene="nrdG"
                     /locus_tag="LNE35_000189"
     CDS             complement(25738..26340)
                     /gene="nrdG"
                     /locus_tag="LNE35_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBK6909380.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26655..28844)
                     /gene="nrdD"
                     /locus_tag="LNE35_000190"
     CDS             complement(26655..28844)
                     /gene="nrdD"
                     /locus_tag="LNE35_000190"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBK6909381.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            29143..29937
                     /locus_tag="LNE35_000191"
     CDS             29143..29937
                     /locus_tag="LNE35_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909382.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29927..31969
                     /locus_tag="LNE35_000192"
     CDS             29927..31969
                     /locus_tag="LNE35_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909383.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            32250..32399
                     /locus_tag="LNE35_000193"
     CDS             32250..32399
                     /locus_tag="LNE35_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBK6909384.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32410..33924
                     /gene="dltA"
                     /locus_tag="LNE35_000194"
     CDS             32410..33924
                     /gene="dltA"
                     /locus_tag="LNE35_000194"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBK6909385.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            33921..35126
                     /gene="dltB"
                     /locus_tag="LNE35_000195"
     CDS             33921..35126
                     /gene="dltB"
                     /locus_tag="LNE35_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBK6909386.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            35171..35404
                     /gene="dltC"
                     /locus_tag="LNE35_000196"
     CDS             35171..35404
                     /gene="dltC"
                     /locus_tag="LNE35_000196"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBK6909387.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35407..36675
                     /gene="dltD"
                     /locus_tag="LNE35_000197"
     CDS             35407..36675
                     /gene="dltD"
                     /locus_tag="LNE35_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBK6909388.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36816..37898
                     /locus_tag="LNE35_000198"
     CDS             36816..37898
                     /locus_tag="LNE35_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBK6909389.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            37898..38701
                     /locus_tag="LNE35_000199"
     CDS             37898..38701
                     /locus_tag="LNE35_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK6909390.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38771..39511
                     /locus_tag="LNE35_000200"
     CDS             38771..39511
                     /locus_tag="LNE35_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6909391.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39522..40526
                     /locus_tag="LNE35_000201"
     CDS             39522..40526
                     /locus_tag="LNE35_000201"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBK6909392.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(40571..42415)
                     /locus_tag="LNE35_000202"
     CDS             complement(40571..42415)
                     /locus_tag="LNE35_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909393.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            42627..43145
                     /locus_tag="LNE35_000203"
     CDS             42627..43145
                     /locus_tag="LNE35_000203"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK6909394.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            43273..43956
                     /locus_tag="LNE35_000204"
     CDS             43273..43956
                     /locus_tag="LNE35_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBK6909395.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            44265..44338
                     /locus_tag="LNE35_000205"
     tRNA            44265..44338
                     /locus_tag="LNE35_000205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44299..44301,aa:Arg,seq:tct)
     gene            44497..44856
                     /locus_tag="LNE35_000206"
     CDS             44497..44856
                     /locus_tag="LNE35_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBK6909396.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            44979..46610
                     /locus_tag="LNE35_000207"
     CDS             44979..46610
                     /locus_tag="LNE35_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBK6909397.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(46672..47313)
                     /locus_tag="LNE35_000208"
     CDS             complement(46672..47313)
                     /locus_tag="LNE35_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK6909398.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    47499..47575
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            47595..47903
                     /gene="rplU"
                     /locus_tag="LNE35_000209"
     CDS             47595..47903
                     /gene="rplU"
                     /locus_tag="LNE35_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBK6909399.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            47930..48274
                     /locus_tag="LNE35_000210"
     CDS             47930..48274
                     /locus_tag="LNE35_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBK6909400.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            48287..48580
                     /gene="rpmA"
                     /locus_tag="LNE35_000211"
     CDS             48287..48580
                     /gene="rpmA"
                     /locus_tag="LNE35_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBK6909401.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            49014..51821
                     /locus_tag="LNE35_000212"
     CDS             49014..51821
                     /locus_tag="LNE35_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6909402.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            52012..52503
                     /locus_tag="LNE35_000213"
     CDS             52012..52503
                     /locus_tag="LNE35_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6909403.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            52606..53595
                     /gene="manX"
                     /locus_tag="LNE35_000214"
     CDS             52606..53595
                     /gene="manX"
                     /locus_tag="LNE35_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBK6909404.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            53628..54431
                     /locus_tag="LNE35_000215"
     CDS             53628..54431
                     /locus_tag="LNE35_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBK6909405.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            54452..55366
                     /locus_tag="LNE35_000216"
     CDS             54452..55366
                     /locus_tag="LNE35_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBK6909406.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            55449..55823
                     /locus_tag="LNE35_000217"
     CDS             55449..55823
                     /locus_tag="LNE35_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBK6909407.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            55962..56207
                     /locus_tag="LNE35_000218"
     CDS             55962..56207
                     /locus_tag="LNE35_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6909408.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            56379..57443
                     /locus_tag="LNE35_000219"
     CDS             56379..57443
                     /locus_tag="LNE35_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK6909409.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            57689..58114
                     /locus_tag="LNE35_000220"
     CDS             57689..58114
                     /locus_tag="LNE35_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6909410.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            58107..58556
                     /gene="nusB"
                     /locus_tag="LNE35_000221"
     CDS             58107..58556
                     /gene="nusB"
                     /locus_tag="LNE35_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBK6909411.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            58653..59507
                     /locus_tag="LNE35_000222"
     CDS             58653..59507
                     /locus_tag="LNE35_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBK6909412.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            59508..60845
                     /locus_tag="LNE35_000223"
     CDS             59508..60845
                     /locus_tag="LNE35_000223"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBK6909413.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            60850..61074
                     /locus_tag="LNE35_000224"
     CDS             60850..61074
                     /locus_tag="LNE35_000224"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBK6909414.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            61076..61966
                     /locus_tag="LNE35_000225"
     CDS             61076..61966
                     /locus_tag="LNE35_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK6909415.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            61975..62793
                     /locus_tag="LNE35_000226"
     CDS             61975..62793
                     /locus_tag="LNE35_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBK6909416.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            62900..63349
                     /locus_tag="LNE35_000227"
     CDS             62900..63349
                     /locus_tag="LNE35_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK6909417.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            63367..65049
                     /gene="recN"
                     /locus_tag="LNE35_000228"
     CDS             63367..65049
                     /gene="recN"
                     /locus_tag="LNE35_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBK6909418.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(65104..65220)
                     /locus_tag="LNE35_000229"
     CDS             complement(65104..65220)
                     /locus_tag="LNE35_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBK6909419.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(65293..66249)
                     /locus_tag="LNE35_000230"
     CDS             complement(65293..66249)
                     /locus_tag="LNE35_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK6909420.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            66420..66788
                     /locus_tag="LNE35_000231"
     CDS             66420..66788
                     /locus_tag="LNE35_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBK6909421.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            66958..67389
                     /gene="mraZ"
                     /locus_tag="LNE35_000232"
     CDS             66958..67389
                     /gene="mraZ"
                     /locus_tag="LNE35_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBK6909422.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            67410..68369
                     /gene="rsmH"
                     /locus_tag="LNE35_000233"
     CDS             67410..68369
                     /gene="rsmH"
                     /locus_tag="LNE35_000233"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBK6909423.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            68374..68775
                     /gene="ftsL"
                     /locus_tag="LNE35_000234"
     CDS             68374..68775
                     /gene="ftsL"
                     /locus_tag="LNE35_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBK6909424.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            68776..70968
                     /locus_tag="LNE35_000235"
     CDS             68776..70968
                     /locus_tag="LNE35_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK6909425.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            70994..71956
                     /locus_tag="LNE35_000236"
     CDS             70994..71956
                     /locus_tag="LNE35_000236"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBK6909426.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            71970..73331
                     /locus_tag="LNE35_000237"
     CDS             71970..73331
                     /locus_tag="LNE35_000237"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBK6909427.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            73357..74445
                     /gene="murG"
                     /locus_tag="LNE35_000238"
     CDS             73357..74445
                     /gene="murG"
                     /locus_tag="LNE35_000238"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBK6909428.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            74460..75680
                     /locus_tag="LNE35_000239"
     CDS             74460..75680
                     /locus_tag="LNE35_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBK6909429.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            75836..77161
                     /gene="ftsA"
                     /locus_tag="LNE35_000240"
     CDS             75836..77161
                     /gene="ftsA"
                     /locus_tag="LNE35_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBK6909430.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            77184..78425
                     /gene="ftsZ"
                     /locus_tag="LNE35_000241"
     CDS             77184..78425
                     /gene="ftsZ"
                     /locus_tag="LNE35_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBK6909431.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            78442..79119
                     /locus_tag="LNE35_000242"
     CDS             78442..79119
                     /locus_tag="LNE35_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBK6909432.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            79133..79732
                     /locus_tag="LNE35_000243"
     CDS             79133..79732
                     /locus_tag="LNE35_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBK6909433.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            79747..80034
                     /locus_tag="LNE35_000244"
     CDS             79747..80034
                     /locus_tag="LNE35_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBK6909434.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            80083..80865
                     /locus_tag="LNE35_000245"
     CDS             80083..80865
                     /locus_tag="LNE35_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBK6909435.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            80886..81593
                     /locus_tag="LNE35_000246"
     CDS             80886..81593
                     /locus_tag="LNE35_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBK6909436.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            81855..84653
                     /gene="ileS"
                     /locus_tag="LNE35_000247"
     CDS             81855..84653
                     /gene="ileS"
                     /locus_tag="LNE35_000247"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBK6909437.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(84702..86228)
                     /locus_tag="LNE35_000248"
     CDS             complement(84702..86228)
                     /locus_tag="LNE35_000248"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBK6909438.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(86243..86890)
                     /locus_tag="LNE35_000249"
     CDS             complement(86243..86890)
                     /locus_tag="LNE35_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBK6909439.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(87074..87403)
                     /locus_tag="LNE35_000250"
     CDS             complement(87074..87403)
                     /locus_tag="LNE35_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBK6909440.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            87580..88308
                     /gene="sfsA"
                     /locus_tag="LNE35_000251"
     CDS             87580..88308
                     /gene="sfsA"
                     /locus_tag="LNE35_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBK6909441.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            88324..89337
                     /locus_tag="LNE35_000252"
     CDS             88324..89337
                     /locus_tag="LNE35_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6909442.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            89337..90614
                     /locus_tag="LNE35_000253"
     CDS             89337..90614
                     /locus_tag="LNE35_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6909443.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            90677..93379
                     /locus_tag="LNE35_000254"
     CDS             90677..93379
                     /locus_tag="LNE35_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6909444.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            93531..93848
                     /locus_tag="LNE35_000255"
     CDS             93531..93848
                     /locus_tag="LNE35_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6909445.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            93878..94198
                     /locus_tag="LNE35_000256"
     CDS             93878..94198
                     /locus_tag="LNE35_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6909446.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            94306..95766
                     /locus_tag="LNE35_000257"
     CDS             94306..95766
                     /locus_tag="LNE35_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6909447.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            95834..96055
                     /locus_tag="LNE35_000258"
     CDS             95834..96055
                     /locus_tag="LNE35_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909448.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            96086..96268
                     /locus_tag="LNE35_000259"
     CDS             96086..96268
                     /locus_tag="LNE35_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBK6909449.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            96268..96681
                     /locus_tag="LNE35_000260"
     CDS             96268..96681
                     /locus_tag="LNE35_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBK6909450.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            96804..97985
                     /locus_tag="LNE35_000261"
     CDS             96804..97985
                     /locus_tag="LNE35_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6909451.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(98056..98220)
                     /locus_tag="LNE35_000262"
     CDS             complement(98056..98220)
                     /locus_tag="LNE35_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909452.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            98374..98447
                     /locus_tag="LNE35_000263"
     tRNA            98374..98447
                     /locus_tag="LNE35_000263"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:98408..98410,aa:Arg,seq:cct)
     gene            complement(98516..99655)
                     /locus_tag="LNE35_000264"
     CDS             complement(98516..99655)
                     /locus_tag="LNE35_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6909453.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(99765..100415)
                     /locus_tag="LNE35_000265"
     CDS             complement(99765..100415)
                     /locus_tag="LNE35_000265"
                     /inference="COORDINATES: protein motif:HMM:NF023043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBK6909454.1"
                     /translation="MKKNLILGFICTSILTLGACSSPSNNATNSETQKTINSLKKENE
                     ELKKQIASQSNDTEENSESIDNASKEKTLPVVGILEYTISSIKTEQVTNDEDNFTNAE
                     YNFSGIENFPEKYYRTTISYKLKNKATSPFSLSSYQASLIDGDGMEYSRTAGEYFLYD
                     ENSNSLVQPETTTSGVFYLLSKNKPNLSTFKLNASKQFTDDFTAEPIGEAGTTEYK"
     gene            complement(100552..101205)
                     /locus_tag="LNE35_000266"
     CDS             complement(100552..101205)
                     /locus_tag="LNE35_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909455.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETNISKTKET
                     TEISSSTSKSTSKTDSSSTVTSSNQATAKPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNTEAQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(101255..101683)
                     /locus_tag="LNE35_000267"
     CDS             complement(101255..101683)
                     /locus_tag="LNE35_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK6909456.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(101688..102062)
                     /locus_tag="LNE35_000268"
     CDS             complement(101688..102062)
                     /locus_tag="LNE35_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6909457.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            102365..102550
                     /locus_tag="LNE35_000269"
     CDS             102365..102550
                     /locus_tag="LNE35_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6909458.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQEHIETVKKILGIQ"
     gene            102563..102901
                     /locus_tag="LNE35_000270"
     CDS             102563..102901
                     /locus_tag="LNE35_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBK6909459.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT        33495 a        17600 c        21617 g        30201 t
ORIGIN      
        1 tattttttac ttatgtgatt cgttaccttc aatgaatccc tacaaggcac ttttgttgaa
       61 aaaaaggtca tctttgaaaa aattagtgga attgttttgt ttttgatatt atttaataga
      121 aaagatatgc caaaaatccg agaactgtgg actacgtttt ttacgtaaag gaggtaaata
      181 agggtgaaag aaaaagattg tttctacgat aagctatata aactgttgct gcaagtagga
      241 aaaagtagtt ctttgttagg tgggatatta tctatcataa tgtttgtttt atttttttat
      301 ctattatttc acttaaaatt tttcagtaac gattttctga tttgcttagg tttaacgtta
      361 gctatgttta tatcaagctt attatgtggg ttaaattttt tttgcactca taaaggactt
      421 aagaaataat ttctcaaagg atatcgaaaa agaaaaaatt gtaagcggat agtattcatt
      481 tgagtacttg actttccctt agtcagcttg tattatacac cttgggcacc cttttagagg
      541 gtcagaagtt tacagaaatt tcggctccct attcttattg aagaataggg agctttgttt
      601 attgtctgag acttttcctg cacataaatt aaaaaggagt ttttgaactt atgacaaaat
      661 atatttttgt gaccggcggt gtcgtttcat caatcggaaa agggattgtt gcagcttctt
      721 taggacgctt attgaaaaac cgcggcttaa aagtaactat ccaaaaattt gatccataca
      781 tcaacgtgga tccggggacg atgagcccat atcagcacgg agaagtattc gtaacagatg
      841 atggtgctga aacagatttg gatctgggcc actacgaacg ttttattgac atcaatttga
      901 acaaatactc caacgtgacg acaggaaaaa tctattctga agttttgcgt aaagaacgta
      961 agggcgaata cttgggagca actgttcaag ttattcctca tattacaaat gagatcaaag
     1021 aaaaaatcat gcgagcagct aaaatgactg attcagatgt gatcattaca gaagtagggg
     1081 gaacagtagg ggatatagaa tctttgccat tccttgaagc acttcgtcaa atgaaagcag
     1141 atgtcggtac agataatgtt atgtatatcc atacaacatt gattccttac ttgaaagctg
     1201 ctggagaaat gaaaactaaa ccaacgcagc acagtgtaaa agagctacgt ggtttaggta
     1261 tccaaccgaa tattttggtg gtacgtacag agcaacctgt ttctcaaagc gtgaaaaaca
     1321 aattggcaca attttgtgat gtcgaaccag aagcagttat cgagtcgcca gatgtagata
     1381 cactttactc catcccatta gttttacaag cacaaggaat ggatcaaatt gtttgcgacc
     1441 atttaaaact agatacacca gaagcagaca tgacagaatg gaaagaatta gaagaacgtg
     1501 tcttgaactt gaagaaaaaa gtacggattg cgcttgtcgg taaatatgtc gagttaccag
     1561 atgcatatat ctcagtagtt gaagcattga aacattctgg tttcgcctat aattcagaca
     1621 tcgagatcga ttggatcaaa gcacaagaat taacgagaga aaatgtagaa gaacgtttga
     1681 aagacgcaga tggaatcttg gttcctggtg gatttggtga tcgcggagta gaagggaaaa
     1741 tcgaagcgat tcgttatgct cgcgaaaatg acgttccgtt tttaggtatt tgtcttggta
     1801 tgcagatggc ttgtgtcgaa tttgcacgta acgtcgttgg attagaagat gcgtcatcag
     1861 ctgaaacgaa tccagataca gcaaacaata ttattgattt aatggctgat caggaaaaca
     1921 tcgagaattt gggtggaact ttacgccttg gattataccc atgtaaaatc aaaaaaggaa
     1981 cgaagacggc tgaagcatat gatggagccg atgttgtgca agaacgtcac cgtcaccgct
     2041 atgagttcaa taacaaatat cgtcaattgt ttgaagaaca agggctagtc ttttcaggtg
     2101 tctcaccaga taaccgttta gtagaaatcg tagagctgcc agagaaaaaa ttctttgtgg
     2161 gatgccaatt ccacccagaa ttgatttctc gtccaaatcg cccgcaaaaa ttaatcaaag
     2221 catttgtcgg agcatcatta gcagatcacg aaacaaaata atttttgaac ttgatagaaa
     2281 gagaaaagta gcaagagaag gggataagca attttcttgt cacttttctc tttttctgat
     2341 ttgttattat ttgtaggaaa aaagatagaa agcaaaagac ttttttcgat taaagcgcta
     2401 ttggatattt tattttttgc aaaaaacttc ttgaaaattt tcattttcat tgattttagt
     2461 agaaaactac cgaaatgttt ggtaaaatag ggtcgttggt taagaattgt cttaacaaat
     2521 ttagttttac aaaacttagg aggaatttta ttatgccagt agtatcagga gctgaatttt
     2581 taaaagctgc tcgtaaaggc ggttacgctg tcggcggatt caacacaaac aacttggaat
     2641 ggactcaagc aatccttgaa gcggctgaag caaaacaagc accagtactt atccaaacat
     2701 caatgggtgc agctaaatac atgggtggtt acaaaatctg taaagattta atcactgatt
     2761 tagttgaagc aatgaacatc actgttccag ttgctatcca cttagaccat ggtgattacg
     2821 aagctgcttt ggaatgtatc gaattaggct atacttcagt aatgttcgat ggttcacatc
     2881 taccatttga agaaaacttg aaattagcta aagacgttgt tgaaaaagca catgcaaaag
     2941 gtatctcagt tgaatgtgaa gttggttcaa tcggcggaga agaagacgga atcatcggta
     3001 acggcgaatt agctgatatt gaagaatgca aacaaatggt tgctacaggt atcgactact
     3061 tagcttgcgg tatcggtaac atccatggtc aatatccaga aaactggaaa ggattagctt
     3121 ttgaccactt acaagcaatt gctgatgctg taggtccaga catgccttta gtattacatg
     3181 gcggatcagg tattcctcaa gatcaaatcg aaaaagctat ctcaatgggt atctcaaaag
     3241 taaatgttaa cactgaattc caattatcat ttgctgcagc tactcgtaaa tatatcgaag
     3301 ctggtaaaga taaagaaggt aaaggattcg accctcgtaa attattagct ccaggtaaag
     3361 cagcaatcgt tgaagatgct aaatctcata tcgactggtt cggttcagct aacaaagcgt
     3421 aagcatccac ataacagata cgatatatag gaatggagaa cgacttttga agtcgctctc
     3481 cattcttttt tacttaaaac ggcagaccat gctcgtttct tttttttatc tatgataaaa
     3541 tacaagcata tccatgaaaa aagattaata aacaaggtgg aaacagatga aaaaaatcgt
     3601 aatcaaagga aaccgacctt tagctggtga agtaacgatc agcggtgcaa aaaatagtgc
     3661 agtcgcattg attccagctg caatcttagc tgattcccca gtgatcttgg aaggtgtgcc
     3721 ggacatacaa gatgttcatt cattaattga aattttagag atcatgggcg caactgtcca
     3781 tttttccaac aacatactag aaatcgatcc tcgagaagtc gtatcgattc cgatgccgca
     3841 cggaaaaatc aatagtttgc gtgcttctta ttattttatg ggaacactgc ttggtaaatt
     3901 tggcgaagcg gtagtaggcc tgccaggtgg ctgttattta ggccctagac cgattgacct
     3961 acacgtcaaa ggatttgaag ctctaggtgc tgaagtgaca aatgaacatg gtgccatgta
     4021 tttacggaca gaaaataaag gattgcgtgg caatcggatt ttcatggacg tggtttcagt
     4081 cggagcaacg atcaatgtga tgttagctgc tgtaaaagca aaaggacaga cagtaattga
     4141 aaacgctgca cgagaaccgg aaatcataga tgtggcaact ttactgaata atatgggggc
     4201 aaaagttcga ggagcgggta cagatgtgat ccgtatcgaa ggagtcgaaa cattacatgg
     4261 ctgtcggcat ttcatgattc ctgaccgcat cgaagcagga acctatctag cattagcagc
     4321 tgcagtagga aatggtatca aagtgaaaaa tgtgatcttt gaacatttgg aaagctttat
     4381 agcaaaattg caggaaattg gtgtccatat gaaaatcagc gaagatgaaa tcgaagttta
     4441 cccctcgaaa gaattaaaac ccgcaaacat catgacctat ccttatccag gatttgccac
     4501 agatctgcag cagccactta ctgcgttact attgatgact acaggcactt cagaaatcat
     4561 cgatacgatc tatgcgaaac gtgtgaacca tgtgccggaa ctagcacgaa tgggcgctga
     4621 tattaccgtg gaaggaaata tgatcatcgt gaatggtcca aataaacttc acggcacaga
     4681 agtcgtggct tctgatcttc gggcaggtgc ttgtttagtg attgctggac tattggcaga
     4741 agggacaacg acgatttaca atgtagatta tatcttacgc ggttatgatc atattatcga
     4801 gaaactaaca gccttagggg catcaattga aatgatagag gaagtgggag ctgaatgagc
     4861 gattatttga caatggcaga actagagaac aaaacgttaa aagaagttta tagttatgcc
     4921 aaagaattta aaattcctta ttacagtaag atgaataaaa aggaattatc tctagctgtg
     4981 attcgtgcac aagcagagaa acaaggattt tattatatgg aaggaatatt ggatatcgtt
     5041 ggtcaagatg gttacggttt tttacggcca atcaattatg gaccaagtgc agaagatatc
     5101 tatatttctg cctcacaaat cagacgattc agtttgagaa atggggacaa agtggcagga
     5161 aaagcccgtc cacctaaaga atcggaacgt tattatggtt tgatgcacgt ggaaagcgtt
     5221 aatggaaaag atccggaaga agcaaaagaa cgtccgcatt ttccagcctt aacacctctt
     5281 tatccgcaaa gacagcttca actggaaaca acaccgcaaa gactatctac ccgaatgatc
     5341 gatatctttt ctccaatcgg ttttggtcag cgtggattga tcgtcgcacc gccaaaagca
     5401 gggaaaactt ctgtattaaa agaaatcgca aatggtatca ccgaaaacta tccggaagta
     5461 gaattaatcg ttctactgat tgatgaacga ccggaagaag taacggatct agaacgaagc
     5521 gtgaaaggcg acgtggtatc ttctacattt gatctgcagc cgcaaaacca tactcgagtt
     5581 tctgaattag tattggaacg cgcaatgcgt ttagtcgaag ataaacgaga tgtcgttatc
     5641 ttgatggata gtattactag acttgctcgg gcctataact tagtcgttcc accaagcgga
     5701 cgtacattga gtggcgggat tgacccagcc gcattgtata aacctaagaa atttttcggg
     5761 gcagcaagaa acatcgaaga aggtggcagt ttgacgattt tagcgaccgc tttagtcgat
     5821 acaggaagcc gtatggatga cgtgatctat gaagaattca aaggaactgg aaatctagaa
     5881 ttacaacttt cacgtgaatt atctgaacgt cggatcttcc cagccatcga tatcaaaaaa
     5941 tcaggtaccc gaaaagaaga attattgatg ccaagtgaag aattagaaga aatttggaaa
     6001 cttcgaaaaa atatgactgg agattcatta gaatatacgg aacaactgat ccgctttttg
     6061 cgtaaaacaa aaaacaatca gcaatttttc aaagaatttc aagatgtctc ctttggtaaa
     6121 aaaaatccga ccagaaatct gaaaagataa ttgcaacctt gactgaaaca tgttaagatg
     6181 ttaatgttgt aaatcgacaa atcaactctg gttcagcaaa tggagcaggg caaaggagcg
     6241 aaaaaatcat gaaagaaaac atccatccag attaccaccc agtagtattc atggattcaa
     6301 caactggttt caaattcttg tcaggttcaa caaaaacttc acaagaaaca gttgaatggg
     6361 aagacggtaa tacataccca gtcatccgtg tcgaagtaac ttctgactca catccattct
     6421 acactggacg tcaaaaattc acacaagcag acggacgtgt ggaccgtttc aacaaaaaat
     6481 acggtctcaa agacgcaaac gctgcggaat aataaaaacg ctgataactc aacgtttagc
     6541 agactatctg atagggatag tctgtttttt tatgtttttt gccaatttaa aacttcgttt
     6601 gatgctgaaa aagtcgtagg atcataatgc acaaaacaat gaaaaactag tatatagttt
     6661 taattatcca cttgataaaa acaccttata ttaacgaaat gaagaggggt tatttcgaag
     6721 taaaaaacca tttacctaaa attagaagct aagctttcct ttcaaggaag gctttttgtt
     6781 ttgttataga cttgcgattg tttgtgagct agatcgttca ttttaatgac ttatacacgc
     6841 ggatgatggg tctttttcct ctgttcattc ataatattga acggtacagc tagcgatgct
     6901 atactgacct cagttccaag aaaacgttaa cattttttgt gggggagagg ttttttatga
     6961 ggtttgatca attgatcgca gaagaactga ttgttttcga tccatcgata gattcaaaag
     7021 gatcattatt tgagcgaata ggggatgtgt tgaaaaaaac aaataaggta agaaatgttc
     7081 aaaaagtcgt taaggcacta ttcaaacgag aggaggagat ctccacagga atagaagatg
     7141 gatttgggat tccccatgca aaaagcaaat atgtgacaaa accagcactg tgtttttttc
     7201 atacagccaa attaacggac tatagcggga tagatgaaat gccaattgaa tatatttttg
     7261 ccatcgtagt tcctgaaaaa tcagcagata tgcatctaga gattctcagc acactctcca
     7321 gacgattgat gaatgcagat tttagaaagg gaataaaaga agccactacg ccagcagata
     7381 tcatcagaca gattagtcaa gaatgaaaat agtagagtct ttgtccgagt atcgatagtg
     7441 aagaaaaagg aggaaaataa tcatgaaaat cgttggtgta actgcttgcc cgactggaat
     7501 tgctcatact tatatgtcgg cagaaaaatt agaagtaaca gcaagaaatc taaaccatca
     7561 agcaaaattc gaaacacaag gtgtaaaaac agaaaatcag ttatctgaac aagaaatcaa
     7621 agaggcagat gcaatcattc ttgcagtaga taaagaaatc gaattggacc gatttgccgg
     7681 gaaaaaagta aagcgtgtct ctacaagccg agcaatcaaa gaaccgcaag tagtaataga
     7741 cgaagcattg agagatattg gcgtattcgt tgtgtctaat gaaaaagaac cagctgcaaa
     7801 tgagaaaaag ccgacaatct ataatcattt catgaatggt gtgaattaca tgcttccatt
     7861 tgtgatcgca ggcggaatca tcatcgcact gagttttgcc tttgggatta cagcggctga
     7921 tcccaactca gcagactata atgtgttagc agctgcattt tcaaggatcg gaggagatac
     7981 agcgtttgcc atgatggttc ctgcgttagg cgcggggatt gctacatcga ttgctggaaa
     8041 agcaggtttt gccccaggaa ttgttgccgg attactggcc tctacaggtg gttcaggttt
     8101 tcttggcgga atgattggtg gcgtgctagc agggtatatc tgtgattttc tggcaaataa
     8161 aattagcgta aaaaaagaat tttctgctat ttatcagctg attgtcgtcc cctttttatc
     8221 gatcttaatt attgggcttt tgatggtatt tgtgattgaa caaccgatca catgggtgtt
     8281 ggatgctttg acaaattggc tgaatagctt gggaaataca tccgggctat tgttcggatt
     8341 acttatcggg atcatgatgg cagctgatat gggtgggccg atcaataaat cgacttcgac
     8401 tttctcgatc ggtttgatgt cagctggagt aaatgcaccg attgctgctt gtatggctgc
     8461 aggaatgaca cctcccctag gtattgcttt agccacttta ttattcaaac ataaatttac
     8521 caaagaagaa cggacttcag gtcaatcatg ctggatttta ggcgcttcgt atattacaga
     8581 aggtgctata ccgtttgcgg tatctgaccc gatccgcgtg atacctagtt tgatgacagg
     8641 ttcagcagtc gctgcaggga tttccatggc agtaggagtt acttcaatgg cacctcatgg
     8701 agggatttgg gtgatgctaa ttccaaatgt tatgaacaat ttacctatgt atgtcttggc
     8761 aattgcatca ggtacagtca ctacagcctt tatgcttgga ctattgaaaa aaacagtgaa
     8821 agagaaaaaa caagacaagt ggacgaaaaa tgaaaatcta acaaatgagg tggaagcgta
     8881 atgttatata caatgaaaga gttattgaaa gtggctaaag agaatcaatt tgcagtacca
     8941 gccttcaata tttgcagcta tgatatgatg aaagcaatca tcgaagaagc agagagatta
     9001 gaagcaccag taatcttaga aattcatcct gatgaaatcg cctatttaag tgatgagttt
     9061 gttgcggcag taagagccta tgcttataca agtaaactgc cgatcgttat ccacttggat
     9121 catggcggta cgatagaaga tgtcttacgg gcgattcgaa acggctattc ttccgtgatg
     9181 atcgatgcgt ctttgaaatc attcgacgac aatgtacaga ttacgaaaaa agtggttgcg
     9241 ttagctcatg cggtaaatgt ctcagtggag gctgagcttg gaacgattgg gaataatggt
     9301 tctgctgaag gaggtgctga aacaatcatt tatacagatc ctgatcaagc gaagattttt
     9361 gtgaaagaaa caggaatcga tacacttgct gtagcaattg gaacagcgca tggcttgtat
     9421 ccgaaaaaca gaactccaaa attaaatatc gacttactga aagaattgaa ccaacaaatc
     9481 gatcttccat ttgttttaca tggcggttca ggtaatccgg acgcagaagt cagcgaagca
     9541 gtaaaatacg gagttgccaa agtgaatttg agctcagatc ttaaaagcgt ctttttcaat
     9601 gctttgagag aaatattgag tgaaaatcca tcgatgtacg agccgaatgc aatttatcca
     9661 gcagctaata aaaaggtaca agaagtggtc aaacataaaa tggaaatttt gaacacagta
     9721 ggcaaggcta gtttatacta aaaataaaaa tgtgtggtca agagggtatg acaatagtct
     9781 tgtcataccc tcttaaccac acacgatgtt cagaagtagt tttttcgaaa aatttcttct
     9841 tatttctcga agccaatcac ttctgtcaca acctcttttt tcctgttact atataaagta
     9901 aatggggtga ttttatgttg aatgaagtgg agaatagaca gcaggagatt gtgaattatc
     9961 taaaaaacaa ccaatttgca agagtcaccg atttgatcga gttagtaaat tatagcgaag
    10021 ccacgattaa acgtgacctt gtttttctgg aaaaaagtgg attgatccgt agagttcgcg
    10081 gtggcgccat gcttattgac aatcgaaaaa ttgacgtgcc ttacctgatg aagatcacca
    10141 aattagaaga agaaacagaa aaacgtaaga ttgcagatac agcttcttct ttgattcgag
    10201 atgatatggt cttattcttg gattccagta caacctcttt acacttagtc agaaacctag
    10261 caagatttga tggtttgcag atcattacga atggcatcgt cacagctgct atgttgagtg
    10321 aatatacaac ggcaaaagta tctgtattgg gaggggcaat tgttccgaaa cgagcaacaa
    10381 tcaacggttc aaaagcctat aacgatgcat tgtcatatta tgcggattta gcgataatga
    10441 gttgtcgcgg gctagatttc agccaaggag taacagaaac tcatgaagga gaggcaatgg
    10501 tcaaaaaagc ttttagaaaa caatcagacc ggcttgccgt tttagttaca cagcaaaaaa
    10561 ttgcacataa attcatgcat caagccattg cttttcatga aatcgattat ctggtgacaa
    10621 gtaaagaact gacaaaagaa cagtatgcga aagtaaagga acaccatatc gaatgtctgt
    10681 acggataaat tattacgtga atgttctaag tggcttcctc atttttagat aggaccttat
    10741 ctttgctcgc ttctctcagc cgatggtata ataatgaaaa ggttatcttg ttagataatc
    10801 ttactttttt atccaggaga aaggagtgca gaaaatgaaa catgtgaagg cgttggtagt
    10861 gaaggcaatc atgatctggg cgattctgtg ggtcgtgctg acaggtttat acggtgttag
    10921 ctttatggat tcaacgattg ttggggtaat tattgtagtc atgatctatg tgctagggga
    10981 tctgttgatc ttacgtaaag tagggaacat cgcagcgacg atcgcagatg ctggttcagc
    11041 tgcagttatc ttgtggctct atctatattt tatgaatgat acagtcgata tttggatgaa
    11101 agtattgatt cctgctctat tgatcggtgt ggcagaatgg ttcttccaca agtggttgct
    11161 aagtcaaggc atcgtaccag atgaacggaa aatggaataa atcaacagtt aaactattct
    11221 tatcgataaa tgaaagcttt aaaaataaat agatgtttaa ttaagaataa tcataaacta
    11281 tttttactca aaaaaccgct cagattattt tcagatctga acggtttttt ttatctattc
    11341 atcaagatca aatttcattt ttcaatgatt caatcaattc gccctctgct tcatgactat
    11401 gttttcggcc attaacaaga tagagtgctg tacatatcaa tggaatgact ccggcaatca
    11461 catacaatcc tctaaatgaa gtaagagatt ttagcatccc catcaaataa ggaccaacgc
    11521 ctaagcctag gtctaaacca ataaagtagg tagataaagc aatactgatt cgatgttcgc
    11581 ggacgatttt taaacaaaca gcctgtccat ttgacataaa cgtaccataa cctaggccga
    11641 ctaatgctcc agagataagc aatgtcacac tattcgtcgt tacactcagt aaaaataatc
    11701 ctgctgttaa gaataaatag ctaggataca tcacgtattg ttctcctttg acatcgaaaa
    11761 tccttccagt caatggacgt gtaaatgtga tgaccaaagc atatacgacg aagaaaaatg
    11821 acccagcagc aactaagtgg attacttttg tataagaaga taaaaatgat aaaacacttg
    11881 aataagaaag acccatcaaa aaggcaattc ctgtaatgaa aagtgctttc ttttcaataa
    11941 aactattgat attccatgag cgaagagata ttttttgttc ttcagataat ggaatatttt
    12001 ttacaggaaa gacaaggcag gcaatcgtaa caaagaagac cagtacaatt gaaaaccaga
    12061 taatgaaacg aaaatcagtc atattcagca aaatcattcc taaaaatggc ccaacagcgg
    12121 cagctaaact agtacttaat ccataataat tgatgccttc ccctttttta gatgcaggga
    12181 tatatgcagt aacgatagca tttgtagctg tagaaactgt accgtagccg aatccattca
    12241 agaatcgaac taaatacaat gccccgatag ttggcgtata aagatatgct aatgttgaaa
    12301 ggagatagaa gatcgcgccg cctcttaagg taaacttccg accaaatagt tctaactgtt
    12361 tccccatgaa taagcgtgcc agcaatgttc cgatgatata gatcccagaa gcaaaacctg
    12421 cttggctcaa gctggcgtgt aaggtgtctt gcgcaatgac tgccatgatg accatcaaca
    12481 gataataaat caggtagaca ataaaattga tcaatgtgat tccaataaaa tatttattaa
    12541 ataatttctt ttccatgtat gttatacatt ccccttaagc tgaatatgct tttaattttt
    12601 gtatttgaac ttctacatcg tgataattgg atggatagcg atcaacaagt tggcgaataa
    12661 ataaatctct tgtagacata aacagattta aaaattcttc gcattcgttt gttggattct
    12721 catccactgc tttgatcaat tcgatcgagt tttgatacgc atcatttaaa aagactaaag
    12781 aagctttcat atcaaatctt gtttgatatg ctgtttctaa tcgattaatg attgtgttca
    12841 tccagtaatt catgtttttt ccctctattc ttaaaaaatt ttacttctgc agtataacga
    12901 ataaaaaaaa ggaaaaaaat cgcataagtg atggaaaaag gtacttaaca aatgttaata
    12961 tcattagtaa aatacctttg gactttaaaa agttttaaag tactgtatga tctttttaga
    13021 tgaaagtttg gatgggggag catcattgga tagtagagtc ttaaaggaat atttggataa
    13081 aaatgatttt ccgattatac gcaaaggata tcataaatat ttgacattcg aagggatgga
    13141 agattcccat acgtatatct tgaaagaggg aacggtaaaa gcaagtgtta tttcaaatga
    13201 tgggagagaa tttaaccttc gctatatcaa agatcttgaa atcgtctctc ttttaaaaga
    13261 cgaatattca caattcatcg attcgccgta taatattcga gttgaatcag aatatgcaga
    13321 gttttatcga atcaatcgtg tgcgcttttg gcaaaatatc aaccaagacc caaagctaca
    13381 acactatgta aaagagtatt accgctatcg tctgctttat tcgatgaaaa aaatgcagca
    13441 aatgctatta aacggaaagc tgggagctgt ttgtacgcaa ctttacgaat tatatgattt
    13501 attcggtgtg cggacagaag aagggatgct gatcgatttt tcgatcacga atgaagaaat
    13561 cgccaaattt tgtggcatct caactgctag cagtgtaaac agaattttaa aacaactgaa
    13621 aaaagatggt attatcaccg ttaaagacca tcatatcgtt attttgaata tggacttgtt
    13681 agaagacaat attatctttt gatgaacaaa gaggttgtga aatcagcgtt ttgacctgag
    13741 tattagagaa acaaacggaa aaatagttcc tcatattttg ccgttttttg agctaatacc
    13801 gagggtcagc tgattgaaca ccgtttacca agaggttgtg agcctgccgt ttaactctga
    13861 gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg tgacttgtga
    13921 ccgaggagtt ggtaggagaa caccgtttac caagaggttg tgaaaaagct gttctgcatc
    13981 aagtaataag aagagccaag taaaaacagt ctcttatgtt tttaacttgg ctggacttat
    14041 caccgtagat gtaagctttt gaacgccgtt tatttgcaac aaaaggggct gtgacacgat
    14101 ttttgtcaca gccctttttt tgcgaacatt catttagcaa tttttagaaa cttctgccgc
    14161 caccgccaaa agtcccgcca ccgctagaat gggtagtact tccgccaccg ccgcttccag
    14221 gaggtgggct tttggggatt cttcttgtcg tgacaaaaga atttgtcaga cgatcggttt
    14281 tttcagttaa tttgatactg gatttttctc ggaatggata tttgtagatg cctgatttca
    14341 attgataacg cgaaacagtc acaaaataaa agacaagact aaggataaga gccaaaacaa
    14401 cagcaatgat gatttctact gtcgtcaaaa ccttatagcg tgtaattttg ccagtttctt
    14461 cgtctacacg ataatgtcct cctggaactc cgtcttccac atatgttgct gatttagaca
    14521 ggtaagcttt tgcagcattg taataggcag catttgtcat ctgatcataa acatcatcta
    14581 agatcgaatc gattctgcta tcagtcaaat aatcgatcat attaccagaa gtcgaaatgt
    14641 agatttcacg ctgtcccata tctaatagca atactgagcc attttgatca tttccgactg
    14701 cgtctcttaa gtaattatca gcaaagtctc ttggttcttc tgaattagat gtagtcgtta
    14761 cgatcaagac tcgtcctttg attttttcat tcagcttttc tgcctgttgt tccaaagatt
    14821 ggatttgatc tgttgtgaat aacccagcct catcatttac cgtagctgta tctgcatgag
    14881 tagccaccgg ccagccaaca cttactagca aaagaaagaa taaaaaaaac tttttcatca
    14941 tatcaagtaa cctccgatca gaaagaggat cgctagccca gcgaaaatcc caagagcggt
    15001 cagtcccaat tttttctggc tgatcggcag gacaccgctg acctttcctg tttgtccatt
    15061 cattgcgtag tagtagactt ttttacttga gtcattgctt cgataagtaa caagccatac
    15121 cggcagtaat acgtaattat ttacttcgtt tgtgattgaa gcagaagttc gcacaccggt
    15181 caatgttgta tatccatttg cggtatcttt taacagcttc tctgaatagt cttttaattc
    15241 agattgaatc tgtgatttga ttgcctcata ttcgatatct cttttttctg cttggaaccc
    15301 ggctaaatac tggcttttaa aatcaattgc tttatctaac gggaaaggtt gcacgccttc
    15361 gaccatcttt tgttgcgtat tttttgataa ggcatttttt actagttctt taaaagaaag
    15421 cttgcctgct cttgaaacag caaattgctt cgtttctgtg tattcgatgt cacctatccg
    15481 ccatatgcgg ataactgtac cattcgcctg catggcacca tctacttcag catctgtagc
    15541 ccaataaggg aaatacacac ctgtcaactt atcgatttga tccttattga aaaaagcttt
    15601 gggaacaaac cattttttct tggtccaagc gagaaatctg ttgattgctt cttctttctc
    15661 gacagcaaaa ggaagtactt tatttggcaa gaattctccg cttaaacgtc ccgatagtac
    15721 gacgggatta tggcagtagt aacaataagt cgcagcagtt gtcgcatcag tcacgatctc
    15781 tgcaccacag ttgggacaaa gaaaaagatc tatcgctgcg gtattatctg cagattcgtc
    15841 cgctgtatct gaaaaaccgc ctgcctcgtc aaccgctttg cgctcggctt cattcatatc
    15901 ggcaagctgt tcttgggctg taaaagtaaa atcttcggta ggtgaaggtt cttctttgct
    15961 ttcttcgcca gccatacgcg cttctttttg cttttgctca tattggctga cttcttcttc
    16021 tgtatagata ttcaggcagt attcgcaatg aaatttttgg tctttgggat caaaagtcaa
    16081 cggaccgccg caattaggac atttatgtgt aagaacatcc atcagctgca cacctttcta
    16141 aataaattaa ttaacgggga ttcctttaaa cggtttacca cagtttggac agaatttagg
    16201 gatgccgttg gaaagatcca cgatctcatt gcattcactg catcgcattt gtagcttagg
    16261 ctgaacgttt tctacaggtt tggcagctcc gcagttggaa cagaattttc cggaattttc
    16321 tgtcccgcaa actggacatg tccaagtatc cgcatttcct tgtgcagttt gttgattttg
    16381 tttttctgct tgttgtttga tttgttcttg gttattttga gctgcttgag aaagataaga
    16441 accatttgca ttcattccca tacctacacc cataaagccg gtcattgctc ctgcttcatt
    16501 ttttccggct gcttccatac cacgtgcaat ggaaccttgc acgtatcctt cacgtacgct
    16561 aggatcacct agcatcgccc cttcgttacg catattgatc agtttcacag aatcatcggt
    16621 gtaagaaatg ctggctacag ccacagaaac aatctccatc ccacgtaatt cacgccaaga
    16681 atcatccaat gcagtatcca tgtatttgct taattccaaa ctcttagatg gtacataaga
    16741 aatccgttga ccatctgcag acatctgatt gattgctgat tgtagcgctg ttaggaattc
    16801 agctaaatac tgctcattga tatcattgat ttccacttgt gttttgtttt tagggattgc
    16861 gtttgtataa aataaaatcg ggtcaacgac atgaatagaa taagtgccat gtgcccgtaa
    16921 gaataattct gcgttataga aattgtcaaa gtagttcaac ggcgcgcttg taccgaattt
    16981 gattcccttt atttcttgga gattgatata aaaaacttgt tgtttctgcg gtgtgacgcc
    17041 gccaaatttg aatcggtcaa aagtttcagc aattgcttct ttcagtgttc cattgaacat
    17101 ggaaggagct gcgtcatttt taatggtgta ataaccttcc tcagcggtat aatcgatgat
    17161 ttttccacca tcgaccaaaa gcatcatcat gtttggatag acatggacga ctgttccgtc
    17221 tgttaaaaca tcttctgttc ctttacgatt ggagccacgt ttgtcatctt tgcggacttt
    17281 gacacctttt gtcatgaccg ttgtatcact catattgtct ggttcgatga cttctagcca
    17341 ttgatctgcc agaccgccgc caaccatact tgttgctgct ttgataagac ccataaaaaa
    17401 ttcctcctct gattcagcta gtgccaaaca attattgttt atttattata ccataggaag
    17461 atgtgaaact tctcggacta gagacggaat tgaaatggaa aattgcgtat tgtttaaata
    17521 cctgaaaaag ccgagaagga tagtagtttt tttacctaat atcacgtata ataagagata
    17581 gtgaattttt taaaaaaggg ggatccttat ggcatatcaa gcactttatc gagtctggcg
    17641 ttcgcagcgt ttcgaagaca tcgttggaca aaaagctgtg acacagacac taaaaaatgc
    17701 tattgtttcc cacaaaacct cacatgccta cttgtttaca ggacccagag gaaccggaaa
    17761 gacgagtgca gcaaagattt tcgcaaaagc aatcaactgt ccgaacagta aagacggtga
    17821 gccatgcaac gaatgcgaaa tgtgtcgatc aattactgct ggtacgcaag aagatgtgat
    17881 cgaaatcgat gctgccagca ataacggagt cgaagaaatc cgatttatcc gtgatcgggc
    17941 aaattatgcg cctacaaagg cagcgtacaa agtgtacatt attgatgagg tacatatgct
    18001 ttcaacagga gctttcaatg ccttgttgaa aacattggaa gagccaaaag aaagtgtcat
    18061 attcatctta gcaacgaccg aacctcacaa aataccagct acgatcattt cacggactca
    18121 gcgatttgat tttaaacgga tcaatacggc agatattgtc gaacatcttg aatttatctt
    18181 gaaagaatcg actatcagct atgaaccagc tgcattgaat gtgattgccc gttctgcaga
    18241 aggcgggatg cgggatgcat tgagtattct tgatcaggca atctcgttta gtgaaggaac
    18301 aattacttta gatgatgcca tgcaagtgac aggtagctta actgacgaaa tgatggacag
    18361 ttacttgagt gcttgcgtga aaaaagaagt accagaagca ttggaaacat tgaaccaaat
    18421 tctcgcagcc ggcaaagaat cacgtcgctt tctagaggat atgttacaat actgccgtga
    18481 cttgctgatg tatcagcaag cacctaacct agttgaggaa caatcgagta cgatcagaag
    18541 tacatttaca gaattggcac aaactgtttc agcggatcag ctgttttcaa tgataaagat
    18601 tttgagcgat actcaaaatg aaattcgttt taccaatagt gccgctattt atttggaagt
    18661 cgctacagtc aaacttgcac agctaccgtc tgcttctgtt ccttcagcag atgtagcatt
    18721 tgcagatgat cagatagtaa aacagctaca acaagagatt aaggaactaa gaaacgaaat
    18781 caaggaacta cgcagtaata caggaattga gaatacaaaa gtacagccgg tacagcaaac
    18841 aaatcataaa aaacagacaa gtacctatcg tgtaccgacg gaacgagtat ataaagttct
    18901 gcgggaagcg acaagacagc atctgaacca agtaaaagaa gtatgggaag acttgctgat
    18961 gagtctatct gtcactcagc gagcaatgct taaagcaagc gaaccagttg cagctggacc
    19021 tacaggacta gtgattgcgt ttgattatga gattgtttgt caaagagcag caaatgacga
    19081 agaattgcag ctttcggtac acaacaactt aagccgaatg atcaaagatt atgcgcctga
    19141 tcctgtttat attacaagag aaagctggcc agaacttcga agaaattatt tgacgcaggc
    19201 acaagaagag ggaacagaaa actcttttgc ttctgaagac tcagaaggag aaatcgagtt
    19261 gcttcctcaa gaacaagaag taccagaaga aacacttgta gtaaaagcag aggaactgtt
    19321 tggctccgaa gctgttacag tggtagatga ttaataaaaa ggagtagata attatgatgc
    19381 gtggaatggg caatatgcaa ggcatgatga aacaagtaca aaaaatgcaa aaagaaatgg
    19441 cagaagcaca agagctgcta aataaaacag agtttacagg taaagccacc aatgaattgg
    19501 tcaaagcggt ttttacaggc gatcgtcgca tgaaagatat ccaaatcaat gaagaagtcg
    19561 ttgatcctga tgatatcgag atgcttcagg atcttgtcgt aatggcagtc aatgatgcga
    19621 tcgcacaaat tgatgtggaa actgagaaaa aattagggaa atacgcaaaa ggtttgccag
    19681 gattttagtt ttaaactgaa ggagtaatga atatgcaata tccagaacca attgcgaaac
    19741 tgatcgacag ctacatgaaa cttccaggaa tcggtcagaa aacagcgaca agactcgcct
    19801 tttatacgat cgatatgaaa gatgaagtag taaatgaatt tgctaaatct ttgttaagtg
    19861 taaaacgcga tttgcatttt tgtagtatct gcggaaacat cacagaagaa gatccctgcg
    19921 aaatatgtag aaatccttca cgtgatcgaa gcgttatcct agtggtcgaa gagccgaaag
    19981 atgtaatggc aatggaaaaa atgcgagaat atcacggact ttatcatgtt ctccatggtg
    20041 tgttatcacc gatggaaggg acaggtccag aagacatcaa tatttcttct ctgttgcagc
    20101 ggctgcatga tgcagatgta aaagaagtga ttatcgcaac aaatgcaaca acagagggag
    20161 aagcaacagc tatgtatctc tcacgtctga tcaaacccgc aggtatcaaa gtgactcgtc
    20221 tagcacatgg tctgtctgta ggctctgata tcgagtatgc agatgaagtt actttattga
    20281 aagctgtcga aggaagacgt gagttataaa tttctaatta agtgtttttt cagatacaat
    20341 ataaaagcag cacaagagat gggggcaaca atgtgaacgg actatttatt acaatcgagg
    20401 ggcctgatgg tgcaggaaaa accagcatct tgaatgaact ttaccctctt ttgaaagaag
    20461 tagcaaaaac agaaatcatt caaacaagag aaccaggagg cattccgatt gctgaagaga
    20521 tccgagcagt gatccttgat ccaaaaaatg atcggatgga tgaacgtacg gaagcactgc
    20581 tttatgcagc agcaagaaga caacatctag tcgaaaaggt tctgcctgct ctggcaaaag
    20641 ggaaaatcgt tttatgcgat agatttgtcg acagttctct tgcttatcaa ggtgcaggcc
    20701 gacgcatcgg cgttaccgag attgctcggt tgaatgagtt tgctacagaa ggtactacgc
    20761 cggattttac cctttatctt gacgtagatt cagacaccgg attaagaaga atcaagaaaa
    20821 acagacaaaa tcaaatcgac cgtttagatt cggaaggttt ggaattccac cagcgtgtac
    20881 gtcatgctta cttaaagctg gcagaagaga atccggaaag aatccacaaa gttgatgcaa
    20941 gaaaaagctt tgaagaagtg ctgcaaacaa gttatcatac aattattgaa cagtatccgc
    21001 aattttttga aaactaggaa ggtgaacgtt tatgaaaatt attttagcga ttatccaaga
    21061 caaagacagc aaccgtttat caaatgaatt gattgatgca aatatccgtg ccacaaaact
    21121 atcatcgaca ggcggcttcc ttaaggctgg aaacagtacg tttatcgtgg gtattgaaga
    21181 tgaccgtgta gaagaagcat tggaaatcat caaaaaaacg tgtgaatcaa gaaagcaatt
    21241 tgtatctaca cctgttacac tggatatttc gatggatggg ggcgtccctt atccagtaga
    21301 agtagaagta ggcggtgcca cagtcttcgt cttacctgtt gaaggattcc atcaatttta
    21361 ggaggagctg agctatgaat caagaaataa acttggctca aatgcaaccg atcgtgtatc
    21421 agcagcttca acggagtttt gagcatgggc gtatcgctca tgcttatctt tttgaagggg
    21481 agaaaggaac aggaaaacat gaagtaggta tatggcttgc acagcacctg ttctgtaccc
    21541 aaatgaaaga caatctgcct tgtggaaaat gcaacaattg tcagcggatc gaaaagaaag
    21601 aacatccaga tgttttggtt attgaaccgg aaggacaaac catcaaagtc gatcagatca
    21661 gacgtttgca gacggaattc agtcgcagcg gttacgaatc aagaaaaaaa gtttttttga
    21721 tcaaagaagc tgaaaaaatg aatagcagtg cggcaaacag tttgttgaaa ttcttggaag
    21781 agccccctgg agatttttta gcggtgttag aaacagatgc agtaggaaga attctcccta
    21841 ctattcaatc tcgttgtcaa gttctgcatt ttcaagaact ttcgaaagaa gcactgatcc
    21901 atcgattgca agaagagcag attccattgg aaaaagcaaa acttttggct ttcttaacaa
    21961 acagtttagg aaaagcagtt gaaatctctc aagatgaatg gtttaatgat gctaaggact
    22021 tgatttatca atggttcgtt tacttgcaaa agcaagatac tcaagccttt atctatgtcc
    22081 aaaaaaaatt ggtaaaatca tttaaagaaa aaacacaaca gtttacagga ttatctgtct
    22141 tgatgtttta ttatcaggaa gcacagcgtc aagcagtagc aaacggcgaa tatggcaaag
    22201 tgaaacagat aaaccagata attgaacgga tattgatcgc agagcagaaa ctacgcagca
    22261 atgtcagttt tcaggctgtt gcggaacagt tcgttttgca tacgattttt tagcttgccg
    22321 caaaggatga tgatcaagga ggatggaaat ggtagaagta gtaggtgtcc gatatagaga
    22381 tgcaggtcat atctattatt ttgcaccagg agactctgaa tatgtttata gtgacaaggt
    22441 attagtcgaa tctcagcaat ccttacatat tgctactgtt gtccttccta aaaaagagat
    22501 tgatcccaat gaccttcctg aagctatccg tccgatcttg cataaagcgt cacaagctga
    22561 attagaaaaa gacaaaaaaa acaaaaaaga tgcttatgat gcattgaaaa tcgcaaaaga
    22621 aaaagtgcag ttgcgtgagt tggaaatgaa aatggtcaaa gccgaataca catttgatcg
    22681 aaataaattg attttttctt tcacagcgga cgggcgaatt gactttagag aattggtaaa
    22741 agatctggcc gctgtatttc gtactcgtat tgagcttcga caaattggtg tccgcgatga
    22801 agcaaagata ataggcggaa ttggtccatg tgggcgtcca ttatgctgtt cttctttttt
    22861 aggcgatttc gtgcctgtat caatcaaaat ggcaaaagat caaagtttat ctttgaatcc
    22921 aacaaaaatt tccggtttat gcggacgctt gatgtgctgt ctgcaatatg agagcgaaga
    22981 atacgagaca gcgaaaaaag aactccctga ctttggaaaa gtaattgcta cacctgatgg
    23041 taaagggaaa gtcgtagggt tgaatctgct tagccgtatc gttagtattc ggctgactgg
    23101 acgggaaaat cctgtcgaat atgaatggga tgaattgaaa gaatttatcc aagtaggtga
    23161 gatgaatgga taagagatca ttatacgacg gattgaatca attagaatca gagttgagaa
    23221 atacattaac agaacttgtt gaaatgaagc atgctttgca tgagatcgta gaaaaaaata
    23281 caactttgga gatggaaaat caacgtctgc gggaacacct gcgggaattg aaccaagttg
    23341 cacaagctcc agctgaaaat gtgaagcagg aactttctaa gtctcgtatg aatcttgaaa
    23401 aaatttatga agaaggattc catgtctgct acgatttata tggatcaaga agggacaatg
    23461 acgagccttg tgcgttttgt ctagaagtga tctatcgcga aagcggtcga tgaccaataa
    23521 caagaggaga ctggagcgga gaaatactgc tccagtcttt ttattaagga gattgtgaca
    23581 gatgtataat caaaaaagtt tcaagggaaa tccggtatat ggggatttat atcttgtacc
    23641 gacgccaatt ggaaatttag aagatatgag ctacagaagt gttcgtatgc tgcaagaatc
    23701 cgatcttatc gcaagtgagg atacgcgtaa tacacaaaag ttattgaatc attttgaaat
    23761 ccatacaccc caaaaaagtt tacatgaaca taattataaa gaacgtgtcc ctcaattgat
    23821 tgctttgtta aaagaaggaa aaacgattgc tcaagtcagt gacgcaggaa tgccttccat
    23881 cagcgatcct ggacatgagc tagttttagc ctgcataaaa gaagggattt ctgtgatctc
    23941 tatcccagga cctacagctg ggatgacggc tttgattgct tcagggctag tgcctcaacc
    24001 atttttattt tatggcttcc ttccacgtaa gaaaaaagaa cagaaagaaa cattgaaaaa
    24061 gctaaaagaa cagacagtta cgctgatttt ttatgagtca ccttatcgga tcactgctac
    24121 attgacgaat tttttagaaa tttttggaaa tcgccaagtc gttttgtgtc gggaattgac
    24181 aaaggtccat gaagaatacc ttagaggaag tacggaagag ctactcgctt atatcgaaga
    24241 acatccagta aagggtgaat gttgtctgct tgtagaaggg aatttgttcg aaatgccagt
    24301 gacggaagtt gatgaagagt tgggaacgtt aaaggaacaa gtagaagcaa agatcgcttc
    24361 aggcgaaaaa ccaaatgctg ctatcaaagc agtagcgctg aaaaatgggc tgaaaaaaca
    24421 agaagtctac cgtcagtacc atgaattaga ctgagatagt ataagttgtc tataatgaaa
    24481 gactaatgag gaaatcagct actaactttt caaatctctt gaacgcgaac gaatgttctt
    24541 gtataatgaa taagaggtga ggatgatgga gctgcaattt cctttacata atgatacatc
    24601 aagaaaaatc attcatattg atatggatgc ttttttcgct tcggttgaag aacgagatca
    24661 tcctgagttt cgcggacatc cattagtaat tgccagacac cccagtgaca ctggtgggcg
    24721 tggagtagtg actacagcaa attatgaagc aagaaaattc ggaattcatt cagcaatgag
    24781 tgcacaaaaa gcgtatgagc tttgtccgca agccatcttt aagcctggcg accatcataa
    24841 atacagcgat atttccaaac aagtacggga agtattctat cgttatacag atattgtgga
    24901 accagttagt atcgatgaag cgtatcttga tgtgaccgaa aataaaatca attgcaagtc
    24961 agccatcaaa atcgcccgaa tgatccaagc agatatttgg gagagtgtcc agctaacatg
    25021 ttctgcggga gtaagttaca ataagttttt ggccaaatta gcatcagact atcaaaaacc
    25081 tagaggaatc acggtagttg cccctcaaga cgctgtgcca tttttaaaag ctttgccaat
    25141 tgaaaaattt catggggtag ggaagaaaac tgtacctaga atgcatgagc tagggattta
    25201 tacaggaaaa gacctatatg aatgcacaga aatgatgttg attcgaaatt ttggaaaaat
    25261 gggctattct ctttatcgta aagtcagagg aatccatgat tctccagtaa acgttacaag
    25321 agagcgaaaa tctgtgggaa aagagcatac ttatgggcag ccgttgcaaa cggaagaagc
    25381 agtgctgact cagttgcgcc aactagctga aaaagtagaa gaagcactga gaagagtcca
    25441 aaaacatggg aaaacagtag ttttgaaggt caggtacacg gactattcta ctgtgaccaa
    25501 aagagtgaca ttgccagaat atatctataa aaaagaagca ctcttttatc aagcaagcct
    25561 tatatgggag gaaatcttgg gagtagaaaa aggaatccga ttgttaggga ttactttgac
    25621 gaatctggac ccgatgactt atgaaaacat cgtccttcca ttatgggaaa atcaagaaat
    25681 ataaaaacag ctttgagagt tgaaaccgga aatccgtttt ctctcccaaa gctgttttta
    25741 tatcaagtct tgctttttga tttgttcata ggtttctgtt gcatccgtac atttttccca
    25801 aatcacaact tgattggatt ctaaagattt ttggacgtca atgatccgct ggttgctact
    25861 tccgcgaaat tgtagtttta ggtcacgttt gcttaattca aagcgtccgt caacaaggat
    25921 atcgatttgc gaaaggagtt ctaatttatc ttctgaatct tgtagcaatt catcaaaggc
    25981 atagcctgtc catgaccaga tatcttttgt cgtaccgaac tcttttcgga tacgtcgtac
    26041 gacttgcaga caaacatctg tattcaaaaa aggttcgcca ccaagtaaag ttaatccttg
    26101 cacgtaatcg ttgcgcaagt cagcgatgat ttgttcttct aattcaggtg tgaatggttt
    26161 gccatatcgg aaattttgtg cagctttgtt gaagcagcct tcgcaggcaa ataagcagcc
    26221 actaacgtaa agactgttgc ggacaccttc accatcgaca aaattaaacg gtttgtagtc
    26281 agcaatatag ttttggctgt agtcttttgc taaccattct tttggttttg gatttctcat
    26341 aaaaaaactt ctttcattta aggttcaaag atatagctat tgaagataaa atctactcga
    26401 ccacataaat aagaacaaac tgacctaaat ccattgttca agaaagcttg ttcttatttt
    26461 gatagagggc gttcaaagat acaaagccat cgaaagaggg tggtgaaaaa agacttgtca
    26521 tagcctctta ttctgaataa acggtgttca aaagcttgca tctgcagtaa taagcccaac
    26581 taaacgaaaa atatgaggaa ctatttttgt ttagctattc ttattacttg atgcaggaca
    26641 gctttttcac aacctcattt catgtgtttc acacgtgagg aaatttcttt atggcggcca
    26701 tgtaccatcg gacgagcttg cggatttcct aaatagccac aagtacgttt gactacatca
    26761 catgtttttg gatcatgatt tccacattct ggacattcga accctcgttc tgtcggtttg
    26821 aaatcacctt cgaaaccaca ttgatagcaa tgatcgattg gcgtattcgt accaagatac
    26881 ccgattttgt catatgcaaa atcccataca gcttctaaag ctttcggatt ttgttggagc
    26941 atagggtatt cacagtagtg gatgaatcca cctgaacaat attttggata atccttctca
    27001 aattccaatt tttcaaatgg cgttgggttt ttacgcacat catagtggaa actgtttgta
    27061 taatattctt tatctgtgat attttcgatg atgccgaatt tttctgtatc caaacggcag
    27121 aaacggtcag ttagactttc acttggagtt gaatagacac tgaaatgata accatattca
    27181 tccccccatg catctgcatt agcttttaat tctttgacga tatcaagtgt gaaagctttt
    27241 gcttctgggt ttgtttccca ttcgccaccg aagaaagctg aagcaacttc atacaatcca
    27301 atgtagccta acgatacagt agctcgacgg tttttgaata attcattgac agaatcggta
    27361 cgtgaaagac gtttcccgaa agaaccatac atatataaaa ttggcgcatt tgctggaatg
    27421 gcttctttac aacgttctac acgatagaga agcgcatctt tcatgatttc taagcggtca
    27481 gacagtaact gccagaattt attctgatct ccttttgatt cgatcgcgat tcttggcaaa
    27541 ttcaatgtaa caacacctaa attcattcgt ccgacattta cttcttgacc attttcgtct
    27601 ttccaccctt gcaagaatga acgacagccc ataggtactt tgaaacttcc agttaattca
    27661 acgattttat cgtaattcag aatatcagga tacattcttt tcgttgcaca ttccaatgct
    27721 agttgtttga tgtcgtaatt aggatcttca gggtttaagt tcacaccttt tttcaaagtg
    27781 aagatcagtt tagggaagat agctgtccgc ttttcactcc ctaatccatt gattcgaatc
    27841 tgtaaaattg ctttttgaat ttctctttca aaccagtttt ctcctagtcc aaatcctaat
    27901 gaagtgaaag gagtttgtcc atttgaagta aataaggtat tgatttcata ttccaaactt
    27961 tgcatagcat cgaagatatc ttttttggtt tttgcttttg catattcttt ttggcgttct
    28021 ggcgaatcga tccattcttc cgcgtctttt agatgttttt tatagttcaa ttctgcaaaa
    28081 ggagcaagca attcatctgt tcggtcggca gaacagccac catattgact tgacgctaca
    28141 ttggcaataa tctgtgagat ttgtgctgtg gcggtctgga tagattttgg cgattcgact
    28201 tcggcattgc cgattttgaa cccattgttc agcatgcctt tgaaatcaat caagcagcaa
    28261 ttcgtcatcg gtgtataagg atgataatcc aaatcatggt aatggatatc gcctttttga
    28321 tgcgcatttg ctatatgtgg tggcaacatt ttcagtccga ttgattttcc aacgatcccg
    28381 gctgtcagat ctcgctgtgt attgaaaaca ttactgtctt tatttgcgtt ttcgttcacg
    28441 acagtttggt ccttgttgat tagcttttca atcgaaaagt tgatatctgt tgctttactg
    28501 cgagcaaaat ctcgtttggt acgataattg atgtactctt ctgctaattc atattcgttt
    28561 ttttctagta atatatgttc cacgatgttt tgaatctcat agattttgac attttcagca
    28621 aaccggcttt tgatttcttg gacaaccaag tctgcaatca tttgaatttt ttcataagta
    28681 agtggtgata atgagccgtg aattttcttt tctgctttga tcagtgcttc attgatcttt
    28741 tgttcatcga aatcaacatg gcggccatcg cgtttgatga ctttcatctg ggatgtctct
    28801 ggctgctgag taacgacagt tttttcttca taatttactt tcattatctt caactcctgc
    28861 taaattgttc ttgtctatca taaaacaaaa tcggggaagg agcaactaaa tatcggctat
    28921 atatagtatt caatgtgctg tataattgga tttctatact atatgtagtg tatattttat
    28981 tgtgaaaaaa ggtgaaaact tgtagctggt ggatttgttt tttgcatatt taattttgct
    29041 gaataaaata attgaataaa attttttatt cagtccaagt aaaagtgaca aaaacataag
    29101 agcaaattat tcgtagatat gctactatgc aaataaatag aaatgaaaaa tgaaagagga
    29161 ggagaagaat tggaaaagat gatcgaagtg aaacatctaa gtaaaagcta cggcagcaaa
    29221 ctttctcccg tcaaagcctt agataatatc tcattcacag tagaagaagg cgagtttgtt
    29281 gggattatgg gcccaagtgg tgcaggaaag acaacgctga tgaatatctt gtccacgatc
    29341 aatttgccta cgatgggcag tgtctcgatc caaggaaaag aaatcacaaa aatgaaaaac
    29401 agtgagctca gtgactttcg tcgaaaaaaa ctaggattca tttttcaaga gttcaatttg
    29461 atcgataccc taaatgcaaa agacaatatc ctattgcccc tagcagttga acggatgact
    29521 aaagaggaaa tggaaaagag agtaagacat gtagctcaat tgttgaatat agaagaactg
    29581 ctcaaacggt atcccgatga actttctgtc ggacagagac aacgaattgc agctgctaga
    29641 gcacttgttg ttcagccaca agtgattttt gcagatgaac caactggctc tttggactcg
    29701 aagtcggcta cagaattgtt gaactacttg aagaccatga atcaaaaaga aaaagcaacg
    29761 atactgttag tcacccatga tccatataca gcaagctact gcgatagaat tttatttatt
    29821 aaagatggtg tgattttttc tgaagttgtc agaagaggga caagaagaga gttctttgag
    29881 aaagtaatag acatgcaggc gacgattggc ggaggaggaa aaatgaatgc tgtataaact
    29941 agccgttaaa agtttttttc ggcagtcacg aggctatctt gtttattttt tcagtttgac
    30001 tttatccgta atgatctatt attccttcag cgcaatgacc tacgatcaat ctttagtcag
    30061 aagagcaagt caagatactt cgatagatgg tggattgagt tttggtggat ttatcattac
    30121 tgttgttttg ctttttttcg tattcagtgc caaccgattt ttcttgaata gaaggcaaaa
    30181 ggaaataggc atctatcagc tttttggtat gaataaactg cagatatcag gtatatatgt
    30241 gttagaaatc atgataattg gattgttcgc ctgtatttct gggatattac tcggtattat
    30301 tttttccaaa ctcttttcaa tgattttggt tcgaatgatg gatatggatc ttactagtcc
    30361 attttttatc tccattccat cagtagtaga tactttattt gcttttttca tcatcctttt
    30421 agcagtatct gtatattcaa tatggaaaat atggcgctat ccaatgattc gaagttttgg
    30481 agaaaaagaa caagcagata gttcaacaat gcgcttcagg acaaggcacc gattactcgc
    30541 agtcatcggg ttactacttt taacgagcgg atattttgga gctagtcatt ttagggaaat
    30601 cacttcatgg atgatcagtt atcattatct tgatacatta gttttctttt ttcctttttt
    30661 aatttttttc gtttgtgtgg tcggtactta tttgtttttc tgttatacat ttcggttatt
    30721 tttgatcata ttgagtaaat ggaagattta ttactatggt atcaattttt tgataatggg
    30781 taacacgcaa gttcatttgc tcaaaagttg gcgtacaaat tctctgatta cagtcattct
    30841 cggaatttct ttaatgacaa ttggcggcac ggctagtatc tctactattt tatcctacca
    30901 ggagaaagtt tcctccccga tggactatca gtttgatgtt gagacagaga aaaaaatcaa
    30961 gccgattcta gcagaggaag ggcagaagat aactaaagaa atcaccttgc attataaagt
    31021 cattgggagt atctatagtc aaaatctcga agaacctatg tttcaaactg ctaatctgat
    31081 cacggaaaca caatatcaag ccttcagaaa agttaatcca gatttgccga acatttcttt
    31141 gaaatcacct ggtcatactg ttttgcttga tcaaatgcaa actctttttc ggaatatttc
    31201 attatatgga tctagtattg gactgccaga taatcaagaa ttgaagattc aacaaatgct
    31261 tccaagtgta ttaggtgacg aaacaatggt atatctaagt cctgtactga ttgtagatga
    31321 taccgttttt gaaaaagcaa aaggtgtgga ttatcaagta gtcaatgtag atgtatcagg
    31381 tggaaataat gaaaaaatag atgaacgact gagcaatgac ctgtcgataa agtggctcaa
    31441 tgctgtttat tattcttatg atatcgtaaa tggaagtcta caaggagaaa ttagcacaaa
    31501 gaaacttccg gatgaccaaa tgaaggaagc acaatttagc caagaaagct ctcgtttgaa
    31561 tttctcttcc agctattcga aattgcgtgc agttaatcgg cgaatcggta tcaatatttt
    31621 tgtcacacta tttgtcggga tgatcgtgat tattgctact ggtagtattt tgacagtccg
    31681 gcaattagca gaagcagagg aagaaaaaga aaattatcac ctattgacta agctggggat
    31741 tccacaaaga aggatcagac ggatgatctt cgagcaaaac gcacttacct tctttccacc
    31801 catgatatta gggatcactc acgcagtatt tgcaatcaat gtcttcactc agtatataaa
    31861 aactgccgat tactggttgg cttatcttgt gagtggctta ttgatcctta tctatctttt
    31921 gctttatgtc attacgagtc agttatattg tcggattatc gaggaatgat atacggaata
    31981 agacaagata ataaatagaa ctaaattttg aataccattt atttggaata agaggttgtg
    32041 acacgatttt tgtcacaacc tctttatttt ttatattttt taaaagtacg agaaattata
    32101 aagaaaaaag cagtgatttg atgtgctatt tttataaaag ctatgtgaca aaactgttag
    32161 taaaaaaacg gtttctgtaa gctagacaca tgtttctttt tgtcaaaatt agttacactt
    32221 tgtgtgaggt tatatgagga gggaatccaa tgaacaaatg gcgttctaat ccaaaattga
    32281 attattgggg aaaattcatc attcgaacaa ttttttatag tgcagtactg ctgttcctga
    32341 tttatctgta tcactataaa aatattcaag gaggaacttt tatctataac gaattttagg
    32401 aggaagagta tggaaatcaa aacgattatt gaagcaatcg atgattgggg aataaatgaa
    32461 cctgatcgag ttgcttatca ggcggatgag acgcatactt ttggtgaatt gaaacaagca
    32521 tcagacgcac ttgcttatta tttagaaaaa aaggtagaag gcgatggacc aattgtcgtt
    32581 tttggcaatt tggagtttga aatgattgtc tcatttctag gggttgtcaa atctggccat
    32641 gcctatttgc cgattgaaga gcatacaccc aaggagcgta tcctatcgat ctttcgggtg
    32701 gctaaaccat ctatggtcat cagtatcggg gactggatag aagatattcc ttcggttcct
    32761 gtcataacaa aaaaagaatt tcaagaaatc aagaagatac cagtaggatt ttatcccaga
    32821 aatagcgtaa aaggtacaga aaatttttat attattttta cttctggtac tactggagaa
    32881 ccaaaaggcg tacaaatcag tcatgataat ctagtcagct ttgttcaatg gacgatggaa
    32941 gactttggga tcaagcctgg tatgcatttc ttagcccaag cgccattttc ttttgattta
    33001 tcggtatttt ctatttatcc tgcattagtt tctggcggaa tgctgaagcc gttaaataaa
    33061 gcagttgttc aagactttcg tcaattattc gcgacattgc cagccttgaa gttgaatgtg
    33121 tgggtttcta ctccatcatt tatggatatt tgcttgatgg aacctacatt caatggagaa
    33181 aatgtgccgg aattggacat gtttttattt tgcggagaag aattgacgaa aaaaactgca
    33241 gaaagtctac ttgatcgatt cccaaatgca cggatatata atacttacgg tccaacagaa
    33301 gcaactgtag ctatttcaag tattcagatt gaccagcatg tactagatag ttatgaccga
    33361 ctaccgattg gctacgtaaa aaaagacacg aaagtttcta ttgtccaaga aggtaaacct
    33421 gtttcaaaag gagaaacggg tgaaatcatt attgcaggac caagcgtatc aaaaggttac
    33481 ctgaacaatc ccgaaaagac agctgctgcg ttcttttgtc aaaatgactt agcaagttac
    33541 catacagggg atgcaggtag actagatgaa tatgggcttt tattttatga aggacgtatg
    33601 gatcagcagg tcaaattaca tggataccgg attgaactcg gggatatcga acattatctg
    33661 ctccaagaca accgaatcaa acaagctgtc gtcgtaccaa aataccaagg aacaaaagta
    33721 cagcagctgg ttgcttttgt cgtattagaa aacaaaacga aacagccgga ttttcagttg
    33781 actaaatcta tcaaagaaca attactagaa gttgtaatgg attatatgat tcctcagaag
    33841 ttccattatg tcgatctgct gcctcagaca gttaatggaa aaatcgatcg aaaaaaatta
    33901 acagctgagg tgaatcctga atgatgttac ctcatatgat cccttatgca gagccgtttt
    33961 atttttttct tttagccatt gcgcttgtac cgatcgttct atctttattg atatgggaaa
    34021 aaagattacc agtttatcaa tcagcagtga cacttttctt tttatttgtc agctttggag
    34081 gtaattattg gcaacaggga gcggcgctga ttgggtatgt tttgtggcag acgatacttg
    34141 tatggggcta ttttgtttat cggcaaagga aaaatcagac aggatggttt tttgccgccg
    34201 ttattttagc gataattcca ctgtttttaa cgaaagtgac tccttttttt gaacatggaa
    34261 aagcatcgtt acttggtttt ttaggaatct cttatttaac ctttaaatct gtacaaatga
    34321 tcatggaaat aagagatggc atgatcaaaa catataagcc atttcgttat atccagtttc
    34381 tgctgttctt tccaacaatc tcttctggac caatcgatcg ctataggaga tttgaaaaag
    34441 atcagctcca tccgcctgtt cgtgataaat atgttgatct tttgggaaaa ggcgtccaaa
    34501 atatttttgt cggtttttta tataaattca ttatcggtta ttattttggt caagtactgc
    34561 ttcctgtagt gggacgcatt gccttacagc atggtggtat ttcatgggca ttagtaggtt
    34621 atatgtacgt gtacagtatg tatttgtttt ttgattttgc tggctatagc ttatttgcag
    34681 tcggtacaag ctacatgatg ggatatcaga caccaatcaa tttcaataaa ccttttttat
    34741 catggaatat caaagaattc tggaatcgtt ggcatatgac gctgtccttt tggttccgtg
    34801 attatgtata tatgcgattg atgttcttca tgatgaagaa aaaatttttt aaaagcaaga
    34861 tcgtaatgtc aaatatcggt tattttgctt tgtttcttct aatgggtata tggcatggat
    34921 tgacgtggta ctacatcgta tatggactgt atcatgcttg tctgatctgt ctgacagatg
    34981 catggatccg atttaagaag aagcataaag agcagttgcc ttctaacaaa tttacgcatg
    35041 gcttagccgt atttttgaca tttcaagcag tatgttttag cttcctgatt ttttcaggat
    35101 ttttagataa actttttttt agctaacgaa tagtgacata ttaatttata taacaaagga
    35161 gaaaaggaaa atggaagaac aaattttatc aatcttagaa gaaataacag gaacagatga
    35221 agtaagaaaa aatcgtgata ttgatctatt tgatgaaggg ttgcttgatt ctttagcatc
    35281 tgttcaatta ttagtcgagc tagacggaga gctggatatc caagtccctg tatctgaatt
    35341 cgagcgagag gattggagca caccgaataa aatcattcaa caagcaactg cattgaaggg
    35401 ttgagtatga aaaagaaact ttttgctatt ttcggcccgg ttgttttagc tgttggctta
    35461 ctgtttgtgt tttttacctc ctcctatcaa gtagatatct ctaatccaga aacgattaga
    35521 cgtgcctctt cttccatgtc agtgaacgtg ttgaaaggag atgcgataaa aaattatgca
    35581 ttatctgaaa aacaatatat tccttttttt ggctcaagtg aattaagcag aatcagtcca
    35641 tttcatccgt ctgttctggc agaaaaatac cagcgaaact atcgtccatt tttgctggga
    35701 gcacccggga cacaatcgtt aagccaatat atgatgatgc gttctgcagg agatgctatg
    35761 aaaaacaaga aagtcgtctt tattatttct cctcagtggt tcgttaaaaa tggtgtaaaa
    35821 actgattact ttaatacgta ttattctgaa cttcaaacat acgactggtt gttttcaatg
    35881 aagaaagtaa cgcctgctga tcgctattta gctagaagat tattaacttt ctcaaaagta
    35941 aaagagaatg atacgttgac ggcgattctg caaacgatca aaaaagggaa gctaccttta
    36001 cctgaatctt tgaatcagct gcgcagccaa tggaacatgc tgaagagaga ggatgaagta
    36061 ttcagcaata ttgggttgaa agatcgacag caaaaaatcg atcatgagtc aaaacgatta
    36121 ccaaaacaat accaagaaac agaattatcc attttagcaa atcaaattgg agaacgagaa
    36181 acgacgaata atccattcgg gttgaaaaac gatttttata cgcatcgtat ccgagcccac
    36241 gagccagagc tcaaacagtc acaaaaaaat tgggattatc gtttttcgcc agaattttct
    36301 gattttcagc tggtcttgga tcaattagca aaaaaccata acgaagtatt gttcatcatc
    36361 ccaccggtta acgagaaatg gagcgattat accggactat ctcaagaaat gttacaagga
    36421 tttgccaaaa agatcaaatt tcaattaaat agccaaggat tcaatcgaat agctgatttt
    36481 gtcaatcaag cgggtaccaa ctattttatg gaagacacga tccatctagg ctggaaaggc
    36541 tggttggctg cagatcaaca gatcagaccg tttttagaag aaaatcatat tactgctagt
    36601 aagtatcatc ttgacgatgc tttttttagc aaatcttggc aacatcaaat accagataaa
    36661 ctacaattaa aataaagaaa acaggcgagt gttgctcgct tgttttcttt atttttgaat
    36721 attctgaata aaagataaaa ataccaagtt tgcctgaaca aaaatcataa gcaatggtat
    36781 aatcacaata tgaatataga aatgggggaa aacatttgga tacaaaagaa gaacagctac
    36841 ttattgattt aacttctgca aaaggagtac ctggaaatga agaagaggta agagaagtat
    36901 tcagagaata tgccaaacca ttcgctgatg atatttttta tgacggttta ggcagtgtga
    36961 ttgccaaaca tggtgctggc ggaggcccca aggtctttat ttctggacat atggacgaag
    37021 ttggttttat ggtaacaaaa ataaccgaaa aaggattctt ggaattccaa actcttggag
    37081 gttggtgggg gcaagtcatg cttgctcagc aagttgaaat caaaacacgt gaagggaaaa
    37141 tcgtccacgg tgtgattggt tccaaaccac cacatgtttt aacacctcaa gtaagaaata
    37201 aaccttatga aataaaggat atgttcattg atattggcgc atcaagccaa gaagaagcca
    37261 aagagtgggg catacgtcct ggagatatgg tcactccata tattgaatac aaacgaatga
    37321 atggcagtaa atatttactg gcaaaagcat gggataatcg tatcgggaca gctgtttctc
    37381 tgcgagtatt agagaatcta tctaaagaag cacacccaaa tgttttattc gcaggaagcg
    37441 atgtgcaaga agaagtaggc ttgcgaggtg ctcgaacaag tacacacctt gtaaatcccg
    37501 atattgcttt tgctttagat actggtacag caggagacac tccaggaatg acaccaaaag
    37561 aagcagattc gattttagga aaaggcccac agattttgat ttttgatgca tctatgatcc
    37621 cgcataaaaa attattgaat tttgttattg atgtagcaga ggaaatggat attccattcc
    37681 aatatacagt gatttctggc ggaggaactg atgcaggaca aatgcatttg acaagagatg
    37741 gtgttccgtc tgtagccatt accgttcctg ttcgttatct gcattcccat acatcagtga
    37801 ttcatgaaga tgattatttg aatacagtga aactagtcac agaattagtg aaacggcttg
    37861 atagtgaaac agttgaaaaa attcggagtt atgaataatg aataatagaa tacctatttc
    37921 tgaatatcgg aaaaaagcac gagtttcttt ggaaaatcaa tggggaatca atacttggct
    37981 tgttttctta tctataatat ttacgagcgt catccagaac ttttttgacg gtttcagtcc
    38041 ttttccggaa gaaagcatgc agcagcactt attgagtttt ctagtcgata atttactttt
    38101 attcgcattc aactatgcat tgttttacat tgcgctagta gttgtacggg gtggcagacc
    38161 aaaaagtaac cttttatttt cagttttcca aagaaagtat tttggttcga tacttggcat
    38221 caatttgatc aattcagtca tcaactggct actcaacagt ttaatctttc tgcccatgtt
    38281 tttaatcagc ggagtgagta cttatatgca gctgcttttc agttcaaatg cggtacctac
    38341 gggtgtcatg ccagaagatt ttcttgatat cagctttgtt ttatcgatga tagggatgat
    38401 ttttgtttca gcactcatca tgagtattgt tggcggttta ttccaatttg ctgcatggac
    38461 aaagttcgac tatcctgatt tgtcggttat tcaatgtttg aaatatggct ggtttttatt
    38521 gaaagataga attggtacgt atattttgtt gcagctgtct tttatcggct ggtatttgct
    38581 aggagcaatc gctttgttca tcggtctttt gtgggtcatt gtttatgtga atgtgtcagt
    38641 tgcagaattt tatgaacaag cacggattga aaaaggaggt ccagacgcct attttactta
    38701 gcatgtaagc agtttattca tcaaaaaaat aacttttgtg gtaggataaa tctttgaggg
    38761 ggaatgtacg atgaaattaa ctgttttagg atgcttagga gcgtatcctt ataaaaatca
    38821 agggacaaca ggttatttgc ttcaatctga cgggttcaat ttgttgatcg atgcaggtag
    38881 tgctacgctg atcaaattac aagaacacct tgatccactt gatctagatg ctgtgatcct
    38941 tagccattat catcatgatc atattgctga ccttggtgta ttacagtatt actggcaact
    39001 tcatcctaag agaaatccgg agacattgct ttcaatttat ggacacacaa aagattccat
    39061 tcactttgaa gaattgacgt tggaaaatgt ctcaaaagga catccttatt ttgaagctga
    39121 agaattaaaa attggtcctt ttctgattac ttttatggaa acaatccatc cagtagtatg
    39181 ttacgccatg cgtatcgtag aagaaacaac ggggaaagta ttcgtattta caggtgattc
    39241 aggttatttg gaaagtttcg ctgattttgc gaaagatgct gatttgtttc tagcagatac
    39301 ctatcttttt gaaggaaatg aacatcatca tgcccatttc acttcaaaag aagctggaga
    39361 acttgcgaaa tcagctaacg taaagaaact tgtacttaca cacctacctc agtttggtga
    39421 tttagaacaa ttgaaaaatg aagcgcaaaa ggcagcaggc aaagggattg atatagaact
    39481 tgctgcagtt gacaaagtgt ttaacattta ggaggaagaa catgattttt gtaccaaatg
    39541 aaaaccacga cccaagaatc aatttagcaa ttgaacaata tttgctgcaa gaaatgcctt
    39601 tagatgaacc tattttgctc ttttatatca atgaaccttc aatcatcatc ggcagaaacc
    39661 aaaatacgct tgaagaaatc aatcgagatt atgtggaaga aaatggcatt catgttgtcc
    39721 gtcgcttaag tggaggcgga gctgtctacc atgattttgg aaacctgaac tttagtttta
    39781 tcatgcctga tgatggggat tcattccgtg actttgcgaa agtaacacag ccaatcatcc
    39841 aagcacttca tgaattgggt gttgctgggg cggaattgaa aggccgaaat gatctagtca
    39901 ttgatggcat gaaattttct ggcaacgcga tgtatgctac caacggtcgg atgtttgctc
    39961 atggaacaat tatgttcgac tctgatatca atgaagtagt caatgcatta aaagtaaaaa
    40021 aagacaaaat cgaatcaaaa ggaatcaaat cgatccgttc tcgtgtaacg aatatcaaac
    40081 catttcttcc agaagaaaag caaaaaatga caacagaaga atttcgtcaa gaaatcttgt
    40141 tgaaaatttt cggcgtagag tcagtagaag aagtaaaaac gtacgaattg actgatgaag
    40201 attggaaaaa aatcaatcga atctctgagg aatattatcg aaattgggat tggaattacg
    40261 ggaagtctcc agatttcaat ttctctcgcc aaaaacgttt ttctatcggt tcgattgaag
    40321 tccatttgaa tgtctcagaa ggaatgatca aagaagcaaa aatctttggc gatttcttcg
    40381 gactaggaga tatctctgac gtggaaaaaa aattggttgg acagaaatat gataaagcat
    40441 ctctatcaaa aatagtcagc caaattgatg tgaagaaata ttttggtgcg atagaaccag
    40501 aagaattgct taccttactt tattgaaaat aaaaaatagc ggaatccaca tatatggatt
    40561 ccgctatttt ttatacagga ttcgggtaat ttcgagagag ttgaacagtg atccgatcaa
    40621 tgaaagtatc tttttcctgc gttagtgttc ctgtgtaacg atccgctacg tctttcatca
    40681 tcattaagtc aaaacgttcc tgatcatcta attcttctgt gatgcctcga ctaaataggt
    40741 ttgtatcgta atgtgcacta tgttcgcaac aaatgacgaa atgttcctct tgttcgtgta
    40801 tcgacaaata aatttttcga tacagtggat tttcgatggc atgagtttct ctaaaggaat
    40861 gctctaaagc gtggatcaaa agttgagcca gatcgtcatc gttgatagga agttcttcgg
    40921 gaagatctgc ttggaaggtt atctgaatat tccgtttggc agctaacttc tggtaacgcg
    40981 ctaagataag atttgtcacc tggtggttgc aaaaagaaca cagcatttcg aaattttttg
    41041 cttcttctaa tatcttttcc acatagtttt tagcttttgt ttggtccgta tgcattaaat
    41101 cttgcagatt ctccatctct tggacaaatt ccaggcgaag tgtatttact tcttggatat
    41161 gctgtcgaag ctgctcataa tgttgctcca acaaatcatt tttgattttt aagaaatcta
    41221 cttgtctccg atctttgtcg gtatttaaga tgtgatcgat tagattgtag ctgataataa
    41281 cgattagaat gataatatat aaaatcgtag accaattgat cgttgtttga tagataccta
    41341 atgccgtttg gatgtaaatc aagcaatgga taatggctac caagacgatc caaggtgtac
    41401 aagctacata aaaacggtta ttcttatatg cttcgccaat gcatgcaagt gaagtgacta
    41461 aagtgcttat aacactagca atattagcaa tcggcattaa ttcaggaagt tctgcaggag
    41521 taaaagttgc ataaagcaga acgccgataa aaatcaaaag ttggaacgta caaaaaattc
    41581 cgtaccattt tttatagtaa aacattcggc tccaatagta gcagatgatg aaagctgacg
    41641 ttaagataat aaataaatga gataaaaaag aatgaacgaa tggttcaaaa agaataccac
    41701 ttaaaatatc ttctgataca gcttcaaatc ctaatgccaa agcaaagcaa cttaagagaa
    41761 taagtgagta ttcaagacgt ttttgtttat acatcaagta gatcgtatag aaaaaggtcc
    41821 ctatagcgat aaaaacagcg attatcatcg tcaatacttg cggcactgat tgtgaataaa
    41881 taaaactaat caatggtcca gttgttccta gataaacttt cggtggcagt ccagcatagt
    41941 tctgataagg agatgagact gtaagggtca atttttttcc gatataattt gctggtaaat
    42001 ctatcacagc caatgagagt ccgggattgc tttcttcctg ataatctttc cgttggtata
    42061 aaatcttttc gcctagatat acagtaatcc attgatggtt tcctttgatt agtaaatttg
    42121 cttcgtccaa cggtttttca agtgtcgttt ccataatgaa tgtttcgtct ttatccacgg
    42181 taggaatcgt acgagtgtac ttgctgtcaa acttaaaatc aggatcatct tttgtataga
    42241 aaacccacga atcttttatt tctttcattt gattgctgtc cgcaggagaa aaaataaaat
    42301 aaaagaaata agcaattcct gttaaaaata acaatgataa tataatgatt atgctccact
    42361 ttatacgtaa ataaaaagat tcttttttat tatatatgtt attaaaaaaa gacataaagg
    42421 tattcctttc taaaacttgt aatgttaggg taaacgtttt ttaaactaac gtcaattatt
    42481 tataataaaa aatgagataa agatgaggaa atagaattag aaacatataa ttattaaagg
    42541 gaaacatttt tacagctata atttgaaaaa aatttatcgt tattatatga ttaagaagga
    42601 ttgaataaag aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt
    42661 ctataacgtt gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt
    42721 ttggtttgac tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa
    42781 atctgatata tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat
    42841 attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc
    42901 acgaagattt ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa
    42961 agagaaatat tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa
    43021 aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa
    43081 tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt
    43141 tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa
    43201 acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag
    43261 aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg
    43321 gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa
    43381 gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta
    43441 cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca
    43501 agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat
    43561 gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact
    43621 ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt
    43681 tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta
    43741 agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca
    43801 gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt
    43861 gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc
    43921 ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga
    43981 taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt
    44041 caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct
    44101 gttttcaatt gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt
    44161 tagtagttgg gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag
    44221 aagcaattta attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc
    44281 tggatagagc atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct
    44341 aaaaaaccgt tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg
    44401 tttgttttca aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc
    44461 aaaaaaataa aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta
    44521 gtgactctag ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat
    44581 tctgacacga caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt
    44641 caaacattgt ttaagaacca aggagtgtac aatggactat taggagtggg actactttat
    44701 ggagcttatc tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt
    44761 tttgttgcat tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg
    44821 ctagctctgt tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc
    44881 taacaggaaa ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat
    44941 ggtattttag aaaatttcat gaaaaaggag ttagacaggt gttcaaaaat tatgtttcct
    45001 cactaaaaaa agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg
    45061 ggatcacagt agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag
    45121 ctgatgcggc cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat
    45181 caggcggttt ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta
    45241 ttgcggcaac aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt
    45301 tacttgcagc aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa
    45361 ttacggggtt tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat
    45421 ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag
    45481 gatttccgat aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta
    45541 tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa
    45601 cagcagttat gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat
    45661 tgatcagtag taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg
    45721 cagttccagc tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt
    45781 cggctggccg aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa
    45841 ttggaaatct gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta
    45901 caagcgtggc catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg
    45961 tcttactgct ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac
    46021 ttgcaggtgt tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc
    46081 tatttactaa taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag
    46141 ttatctttga tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg
    46201 ttgtcaaaag cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc
    46261 tgccagaaac ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt
    46321 ttatgtcttc agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca
    46381 ttttctccat gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat
    46441 ttcaagaaaa agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag
    46501 tagaaaaaca actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg
    46561 ctcatgcgtt gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa
    46621 aagaacagac aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg
    46681 ctgtaaaagt gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt
    46741 gtagaaaata gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc
    46801 gtaggcatcc ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt
    46861 tttggtaacc gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc
    46921 aattaatttt ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac
    46981 ttcggaacga gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt
    47041 gatccgatga gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc
    47101 tcgtgttaag ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa
    47161 aggaacatgc gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg
    47221 atgaagaggt ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga
    47281 agcttcttgt atcagttctt ttgggtattc catttatgct cctccttttt cttttattat
    47341 aagaataaaa gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc
    47401 agaagtattg cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt
    47461 gctgttctgg taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa
    47521 caagtaataa gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg
    47581 aggtgcttta tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag
    47641 tgggacaagc aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg
    47701 acgaagttat cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg
    47761 caactgttga aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat
    47821 acaaacctaa aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg
    47881 ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg
    47941 aacatttaag cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga
    48001 tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac
    48061 tgtcaacggg attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga
    48121 aggcggttat ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc
    48181 tgagattcta ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga
    48241 atatatccaa attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat
    48301 gaatctacaa ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc
    48361 agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat
    48421 tctttaccgt caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga
    48481 cgacacttta tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa
    48541 aaaacaagtg tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga
    48601 ggttgtgaca agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga
    48661 ggctacgaag tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg
    48721 atgttttgat aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat
    48781 aattatatgg taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt
    48841 ttcgttcttt taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag
    48901 gtaaatagtt gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa
    48961 tagtctatag aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg
    49021 tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg
    49081 aaattgctga agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag
    49141 tgcgcgaagg aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac
    49201 tcgttacgca cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct
    49261 ctgaagaaaa aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag
    49321 ctaacggcag catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa
    49381 gaggattaaa ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg
    49441 cgatgtatca ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt
    49501 tcaattgtgc tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg
    49561 caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg
    49621 gcggtatgct gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct
    49681 tttactttat ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag
    49741 caaatgtacg gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt
    49801 ttgttcggcg tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg
    49861 agaaagtcga tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga
    49921 tttctttgac agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag
    49981 gacaattgaa gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat
    50041 cacccgaaat ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc
    50101 aattagcaag caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa
    50161 ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata
    50221 atctatatga tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag
    50281 aagcaatcaa tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag
    50341 accatggttt ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca
    50401 tagaagtgac caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc
    50461 aaaactttat ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag
    50521 gagaagaacg gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat
    50581 atgctgttgc gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag
    50641 aaagtgaaga ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg
    50701 gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag
    50761 ttgtccaaca gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg
    50821 atccgaaaac agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg
    50881 gggtcatact cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag
    50941 acactggaat tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg
    51001 tacgaaaggt ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt
    51061 ttcgaggtta cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata
    51121 agaaaaaagc tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag
    51181 aattgatcga acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt
    51241 cgattgtgga agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta
    51301 ctactgggat tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg
    51361 atcaaaatat cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg
    51421 aattcatctc cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata
    51481 acttcatctt tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa
    51541 tctctcagac gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc
    51601 acacagcagg aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg
    51661 aattgacaaa tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt
    51721 tggaagatca gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag
    51781 tccataatca gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac
    51841 ttttatagtg tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc
    51901 gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca
    51961 cagaaatgat aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt
    52021 cgattggcac gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag
    52081 caaacaggtg ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga
    52141 aagtttctac taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag
    52201 aaaatgattc aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc
    52261 accaatcccc aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat
    52321 atcggcggta tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat
    52381 caacaagata ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga
    52441 aaagtacctg ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg
    52501 tagcaagaat ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta
    52561 atatttttga atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc
    52621 ctagcaagtc atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc
    52681 gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga
    52741 gccaaattgg aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt
    52801 gatttatggg gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat
    52861 aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc
    52921 ttttcaatgg aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt
    52981 gtaaaagtaa aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac
    53041 gcacaaccta aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat
    53101 gtattagcac gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa
    53161 gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt
    53221 aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc
    53281 aaaatgatcg aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt
    53341 gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat
    53401 gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga
    53461 caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt
    53521 aaagtaccta atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa
    53581 ttagcaaaag cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt
    53641 caataatttt agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat
    53701 ttcaatttca tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg
    53761 aagctggtat cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg
    53821 gtgccgctgt agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt
    53881 taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg
    53941 ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga
    54001 tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg
    54061 tatgtttaca aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac
    54121 aaactgtgca atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg
    54181 gtgggggaat ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag
    54241 aagtatggcc attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga
    54301 ttgctctagg tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg
    54361 gtggttcatc aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga
    54421 atgactatta atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa
    54481 aaaagatcgt ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga
    54541 acgtatgcaa aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa
    54601 aacaaaagaa gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc
    54661 atacattgct tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg
    54721 cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc
    54781 tggtgttggt gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc
    54841 ttcattagct atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat
    54901 tattcgttgg gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat
    54961 tactgatgac ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg
    55021 gatgttcgta ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt
    55081 atcaactgta aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg
    55141 tgaaggaatc aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa
    55201 ggtaacaaca ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt
    55261 aacattgtta tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg
    55321 tttattcgta gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata
    55381 gctcgggcgt tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga
    55441 gggagaaaat ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt
    55501 ttacgggtct gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc
    55561 atgcgcgaga tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag
    55621 catcagtcat gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg
    55681 gtacatacac attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg
    55741 tagatgaaga tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc
    55801 gtagtatgtt taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat
    55861 ttttttactt tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat
    55921 cccatatgct aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat
    55981 tgattgttgg agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga
    56041 tgggctgtgt aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt
    56101 tcggcgaatg gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag
    56161 tgattttggt agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat
    56221 agtaaactaa aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa
    56281 taaaaagaag ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat
    56341 aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat
    56401 tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc
    56461 ttcgttactt aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag
    56521 cattttttat tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg
    56581 aaattattaa aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag
    56641 aactgcactc attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag
    56701 aagaaatcat cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag
    56761 tgaaagatga agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat
    56821 atgatcacat cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac
    56881 tagactttta tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta
    56941 gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg
    57001 acttgatcac attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta
    57061 cattcgctat tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg
    57121 ctcaattagc agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag
    57181 ttgctagaga ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac
    57241 atgggatcgg tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat
    57301 ttgtaccagg aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag
    57361 tacgtattga ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac
    57421 caaaagaatt gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt
    57481 tcacagactt tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa
    57541 gctgtaagag ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg
    57601 gcggcagttt tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc
    57661 gtgcttaggc aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc
    57721 aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat
    57781 tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac
    57841 tgaattatta ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg
    57901 cttgaaagta gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat
    57961 ggaaatgcag gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga
    58021 agtcaatatc catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga
    58081 actttttgaa gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg
    58141 aaatggcatt gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg
    58201 cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt
    58261 ttgttcctgt ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg
    58321 ataaagtgat tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg
    58381 atttggtgat cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg
    58441 ctgttgcttt aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta
    58501 aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc
    58561 tagctttttt ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa
    58621 tgaaaacaca aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat
    58681 tagctgaaaa aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta
    58741 tccatcccgg attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa
    58801 gaaataaaga acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg
    58861 agacgatttc tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt
    58921 ttcatggtat attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt
    58981 tagctatccg ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc
    59041 ttgcgggtaa accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag
    59101 cttattctat cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg
    59161 gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta
    59221 aaaccgaaaa tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac
    59281 gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga
    59341 tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta
    59401 ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga
    59461 tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat
    59521 atttaactgt cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt
    59581 tagaacgcgt atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc
    59641 aatatttcag cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat
    59701 tccaaaaatt acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt
    59761 cattatatga agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa
    59821 taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt
    59881 ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc
    59941 caagcggagc ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc
    60001 aattggtttt gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga
    60061 atatccagag agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg
    60121 gttcgattga ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag
    60181 ctaatacgcc ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag
    60241 tagcagatgt tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt
    60301 cagaagagtt gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc
    60361 agatccagcg caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc
    60421 ctgaaagatt gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc
    60481 aagctacaca gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat
    60541 tggaacaagt ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag
    60601 aaaaacgctt agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc
    60661 aagctgttca atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct
    60721 atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc
    60781 tgacgatcca ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag
    60841 aataaaaata tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc
    60901 atgcagttag aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga
    60961 atggaattaa gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa
    61021 atgatgacag aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag
    61081 attaactgac ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt
    61141 agatgagcat acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg
    61201 cgggaaaaga atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat
    61261 tgatgttccc gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat
    61321 ccacgatgat ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca
    61381 taaagtattc ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt
    61441 ccagctaatc agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt
    61501 agctgtttgt gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga
    61561 atcgaaaaag ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt
    61621 gattcgttat gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt
    61681 gctacttcaa cgattagccg aacatttagg tttagctttt cagataagag atgatctact
    61741 cgatgtgatt ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga
    61801 aaaaaatact tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga
    61861 gctgttatcc gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg
    61921 cgggctgatg cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa
    61981 aaagaacgcg tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc
    62041 aaacgttcag tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg
    62101 ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt
    62161 tctcgagggg gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga
    62221 aaaacattgt tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat
    62281 ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga
    62341 caagatccta gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac
    62401 tttaccttag gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg
    62461 atgctgcctc cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa
    62521 ccccaattcg aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag
    62581 acccatcaaa aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg
    62641 ctggaattaa gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac
    62701 ttgaggaaag tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt
    62761 tcaaatgcac acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa
    62821 gtatgcggat tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat
    62881 gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt
    62941 gagtgagcaa gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat
    63001 atctgttact caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc
    63061 ggcttcagat ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac
    63121 taaattggaa aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt
    63181 tattttgaaa acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta
    63241 caagaaagaa ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac
    63301 tgaagaccag gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg
    63361 aatgtgatgc tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt
    63421 tcttttcacg aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt
    63481 atcgatgctt taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga
    63541 gcagaaaaat gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa
    63601 ctgatggttg aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg
    63661 tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg
    63721 agaagaatcg gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc
    63781 cagcctgaat cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag
    63841 aaaaagagtt atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa
    63901 gtgcagcaaa atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa
    63961 gaaatagctg ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa
    64021 aaacttagca attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg
    64081 aataatgaac aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc
    64141 gcccatctag atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg
    64201 ctgcaagatg caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt
    64261 cgtttagaag aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc
    64321 gaatccattg agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct
    64381 gatttttcag aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta
    64441 cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa
    64501 aaatccattc tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt
    64561 ttcttaaaag aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac
    64621 attacgacca atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa
    64681 ctgtctcgga tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt
    64741 attatttttg atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat
    64801 aaaatttctc aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt
    64861 gctgcagtcg ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa
    64921 acttctgttt cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct
    64981 ggaagtgaga ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa
    65041 aggacctgag ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta
    65101 tttttacatg acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat
    65161 tgtccaaatg acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat
    65221 tgttttcaac ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac
    65281 aagagggctt ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat
    65341 aaataatgtt aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt
    65401 aatgttcatt ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga
    65461 agttaaaatt ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt
    65521 catttgttct actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac
    65581 caatacatca cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc
    65641 tagttctttc aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt
    65701 ttggatatcc atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc
    65761 caaagtatcg cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg
    65821 ccaaatgaat tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg
    65881 gctaaagact ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt
    65941 gggcgtcaaa ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt
    66001 tttagcaaaa ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa
    66061 ttcttctgta cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat
    66121 ttcaaattga tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca
    66181 gatcgtttgg tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata
    66241 tttgatcatg attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga
    66301 atacatggaa tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa
    66361 cagttgtgta aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca
    66421 tgggcacatt cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg
    66481 gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg
    66541 atttggcgat tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa
    66601 tttctatctt accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt
    66661 tccaagcata ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt
    66721 tggtttttct gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt
    66781 acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat
    66841 ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta
    66901 gaaagtgggg gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg
    66961 ttgatgggtg aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa
    67021 cttcgtgaag aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc
    67081 ggctatccga tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct
    67141 aaaaaagacg cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt
    67201 gataaacaag gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa
    67261 ggttgcgtag tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa
    67321 gatttttctg cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc
    67381 ggactataag aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca
    67441 tgttgaaaga aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta
    67501 cactcggcgg tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc
    67561 tgtatgcgtt tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt
    67621 atgtcgaaca aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct
    67681 tgagtgagca tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc
    67741 agctagatga agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca
    67801 tggatcagtc agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat
    67861 tagtaaagat tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa
    67921 tcgaacgagt acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta
    67981 aacaagtcat cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc
    68041 aagctgtgcg gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg
    68101 caatccggtt attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag
    68161 acagaattgt caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac
    68221 ttcctgtcat acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc
    68281 ttccttcaga ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg
    68341 cagaaaaaat aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat
    68401 tacgaatatg atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct
    68461 tcgaatcaag aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct
    68521 ttggaaaaaa ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca
    68581 gttatgctgc gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa
    68641 atcaatgata aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt
    68701 actgaacgga ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta
    68761 aggaaagtga agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat
    68821 ctaaatccga tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga
    68881 ttgttctttt tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt
    68941 acctcattag ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa
    69001 agaggaacga tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca
    69061 attaaagcga tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa
    69121 aagaattttg ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat
    69181 gcactgaata ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc
    69241 tatgggaaaa atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa
    69301 ggtgtcgctg gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt
    69361 tcttctcatt ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa
    69421 ctaggtttgg agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat
    69481 caaaaagata attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc
    69541 gacggacaag atatctacac gactttagac agcagactac aaagttattt agaaacactg
    69601 atggatcaag taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca
    69661 aaaaccggag aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa
    69721 ggattgactg gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca
    69781 ggatcaacca tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag
    69841 aatgaaacct tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac
    69901 tttggtgaaa aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg
    69961 atggtcatac tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga
    70021 tttggacaaa gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta
    70081 aatattgtcg accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa
    70141 atgatgaaag ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc
    70201 agcaaagtgg tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg
    70261 caaccttttt caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa
    70321 agcgaaaact acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca
    70381 aaaaacggaa cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc
    70441 tacctgtatt cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta
    70501 acaatgaaac accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg
    70561 atgaaacggg caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca
    70621 gaaaaagtat ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct
    70681 caaaaaagtg gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct
    70741 actgccaacg gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat
    70801 aagctgatgc ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc
    70861 ctcggtattg aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca
    70921 tatgaaaaaa tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag
    70981 gagagaaaag agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat
    71041 gacaattgcc gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc
    71101 gattcgtgag gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg
    71161 cttagtgttt ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca
    71221 gctaactcct actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt
    71281 tcttgatgat tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa
    71341 attgattgga cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta
    71401 tccaggtgta ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt
    71461 cgctctcttc tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg
    71521 attagttgct ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca
    71581 gcatcagtat gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt
    71641 cgtctacaac agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg
    71701 cggactttta gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg
    71761 tttgatctat gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac
    71821 aggaaaacga atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc
    71881 ggagtggaag attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt
    71941 atggatcgtt tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga
    72001 aagtgcttgt cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat
    72061 tgggcgcact tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag
    72121 agctgctttc tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg
    72181 aagaattttc attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa
    72241 aagcccaaga aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg
    72301 aatgcccaat tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag
    72361 gactactact gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt
    72421 atccagcaag tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt
    72481 ctagcttcca actcatggga atcacagatt tccgccctca tatagcggtg attaccaata
    72541 tctatgaagc acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga
    72601 accttcaaaa aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac
    72661 tacaagaatt agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt
    72721 tggaagacgg tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg
    72781 aaatatctga acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt
    72841 ctgtagcaaa attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc
    72901 atggcgtacc acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg
    72961 attcaaaagc aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta
    73021 aggtcgtgtt attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt
    73081 cattaaaagg aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg
    73141 ctggaaaaaa agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc
    73201 ctttagcatt agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca
    73261 gctgggatca atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc
    73321 gattaaaata gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga
    73381 ggaacagggg gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa
    73441 ccgaatgctg aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct
    73501 gatacaggaa tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg
    73561 cataacatca agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc
    73621 agtgattttc aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg
    73681 tacgctgcag ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt
    73741 gttaccaata aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg
    73801 gcttcctttt ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta
    73861 gcagatacta aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca
    73921 gtcttgattt ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt
    73981 ttaagtgaat atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat
    74041 gaagagatcc taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc
    74101 aaaaatatgg cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact
    74161 tcgatcgcag aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt
    74221 acgaatgatc atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg
    74281 attgcagata atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt
    74341 gatgaagaat tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct
    74401 tcaaaacgtc tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag
    74461 tgagtaagaa aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga
    74521 ctccttggca aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc
    74581 caaaaaaaga agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg
    74641 ttcaagagga acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga
    74701 tcaagcaaaa gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt
    74761 taaaaagtca acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg
    74821 gtataccgct gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg
    74881 cagtatcagg caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat
    74941 taggagaaaa tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa
    75001 aagcacagcc aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt
    75061 tggatgtaac agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag
    75121 tgattgaaaa tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta
    75181 tcttggaaga gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt
    75241 tatcatcaga attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata
    75301 aaaatctgat ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc
    75361 ttgcgaacca aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga
    75421 tcgacatgga agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat
    75481 cgggccaatc ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa
    75541 gtgaagaggc agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat
    75601 ctgattcaaa tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt
    75661 caagtttaaa tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac
    75721 cagtaattta tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa
    75781 cggacgctag ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc
    75841 aaaaacagga atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc
    75901 tgaatatatc gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat
    75961 taaccgaggc attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg
    76021 acaagcagaa gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa
    76081 catgcttgaa gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat
    76141 caccgatgaa gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga
    76201 acgtcaaatc gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa
    76261 agatcctcgt ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc
    76321 taaaacgatc atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga
    76381 aatggtgatt actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt
    76441 tggtacgatc gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa
    76501 gcaattgaag tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc
    76561 tattgtatta aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc
    76621 atacccagaa cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga
    76681 accagtgaaa gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat
    76741 tttcaacaaa gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt
    76801 cgtattgaca ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt
    76861 cggcgtaaat gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac
    76921 aaatgtaata agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa
    76981 aattgctgtt acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac
    77041 aaacacatat gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa
    77101 agagtccgga gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta
    77161 attcatcctg ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg
    77221 cagtaatcaa agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg
    77281 aagaaaacgt taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa
    77341 attcaaaagc tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg
    77401 gttctcagcc agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag
    77461 ctttagacgg tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag
    77521 gtgctgcacc aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg
    77581 tgacacgtcc attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg
    77641 ctcgtttgaa agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag
    77701 ttgtagataa aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac
    77761 aaggtgtaca aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg
    77821 ctgacgtgaa aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta
    77881 gtggagaaga tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa
    77941 catctattga tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat
    78001 tgttcgaagc acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata
    78061 tcattctagg tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg
    78121 caacaggtat cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac
    78181 aaatgcaatc acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt
    78241 cagaagaaga aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta
    78301 gagttgatga ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg
    78361 aagaaacaaa atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa
    78421 aataaaggag aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt
    78481 caggattcgt gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca
    78541 aaatctgttg aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca
    78601 gaaaaccgag tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata
    78661 aagtggcatt taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt
    78721 gattactttc atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag
    78781 aaagaattat cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt
    78841 tcaccagatg agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt
    78901 gttgggttga tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt
    78961 ggtacgctga aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt
    79021 agtgaattaa gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca
    79081 tttatccgtg taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct
    79141 tttaaacaga ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata
    79201 ccaagattat cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca
    79261 gaatcaatca actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac
    79321 ttcacgtcct gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact
    79381 acatcaatca cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca
    79441 acctaaagca aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc
    79501 tatggcgatt gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt
    79561 agaagaaaaa caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga
    79621 tggagatatc aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat
    79681 tgacagttca accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga
    79741 actacgattt atactattgc actattactt catttacttc aacgggcagc ctatttctat
    79801 agcattatct tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg
    79861 ctaggacgat tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct
    79921 ttgaggattg gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc
    79981 gtacaaggtt tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag
    80041 caacaaaatc tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac
    80101 cagcactttc gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag
    80161 gttcaaggac aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt
    80221 ttagaaacac tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa
    80281 caggcagaaa gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat
    80341 tttgaaacag cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg
    80401 aaaatccttg gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc
    80461 tcggacggtg atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca
    80521 caagtaacaa agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg
    80581 attatgccga aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac
    80641 agtgtgatct ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct
    80701 ggtaaagtaa aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta
    80761 gatattattt cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg
    80821 aaaaaagaaa agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt
    80881 tactcatggc tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg
    80941 gatataatca agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat
    81001 tgactcaaag aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt
    81061 acttcaatga attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg
    81121 ataaagtaaa aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca
    81181 aagcagatga gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag
    81241 aagaaaaagc aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag
    81301 aaactgaaga tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg
    81361 agtctcaatt agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca
    81421 gctacgtgac tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg
    81481 aaaatgatac tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg
    81541 atacacaggc tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa
    81601 aagcactttg ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat
    81661 ggtgaaagat cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg
    81721 acctcgttat tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac
    81781 cacggcatgt tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag
    81841 gagtgtatgg ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta
    81901 tgcgtggtaa tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa
    81961 tctaccaaaa aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac
    82021 ctccatatgc caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca
    82081 ttatcattcg ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata
    82141 cacatggatt gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt
    82201 ccatggcaga atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac
    82261 gagcagattt caaacgccta ggagttgcag gagattggga acatccttat gttacattag
    82321 atccttctta tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata
    82381 tttacaaagg attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag
    82441 ctgaaattga atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag
    82501 acggcaaaga tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga
    82561 cattgccagc aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag
    82621 ccgatggccg taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg
    82681 gttgggaaaa tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg
    82741 caagtcatcc attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag
    82801 acgctggtac tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg
    82861 gtagcaaata taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag
    82921 aagcacctgg atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat
    82981 taaaagaaaa aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt
    83041 ggcggacgaa aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata
    83101 aattccgtca gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta
    83161 aaacacgttt gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg
    83221 catggggagt accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag
    83281 aaacaatcga acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa
    83341 aagaagcgaa agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat
    83401 ttacaaaaga aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg
    83461 ttttacgtca gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc
    83521 aatatcgtgg ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac
    83581 catacaaatc agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta
    83641 aatctttagg aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc
    83701 ttcgcttatg ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct
    83761 taagtcaagt ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga
    83821 atacagaaga tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag
    83881 acaaatatat gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg
    83941 acaaatataa cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat
    84001 cgtcattcta cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc
    84061 aacgtcgttg catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga
    84121 ctcctatcat tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt
    84181 acgttcagtt aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg
    84241 atacctggac tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa
    84301 gaaacagcaa attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac
    84361 aagttcgtga aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac
    84421 cagactactt tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg
    84481 atgatgttgc tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag
    84541 ttcgtaaaga tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa
    84601 tcgttgaaga cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa
    84661 aagggggtgt gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca
    84721 cgttcacgat accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt
    84781 tcgaaagctg ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt
    84841 tggtcccaag cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa
    84901 tgagagaagt tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct
    84961 gggctatttt caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca
    85021 aatccttgcc ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata
    85081 tagatcgtta gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt
    85141 ttgaatacat taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc
    85201 aaacgtttgc ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg
    85261 aatttgcctg cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga
    85321 tagcccttga attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt
    85381 tctaacactt cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta
    85441 cggatttctt tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt
    85501 aaaatatgat tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga
    85561 tcttctacac cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc
    85621 cattgagcct caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct
    85681 tttcctaaat aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc
    85741 tggttcaatt caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga
    85801 ttgaagccat tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa
    85861 aattgaggtg ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct
    85921 aatcggtcag acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat
    85981 tgatagtaaa aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg
    86041 attgtttctt tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg
    86101 attacagcaa aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac
    86161 aatttacgct gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca
    86221 ttagacatgt ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa
    86281 agctgacggg gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga
    86341 tgtcaacgat ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca
    86401 attctttttc atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca
    86461 atgtttgtcg gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag
    86521 gacaatgttc aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca
    86581 catgttccaa tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa
    86641 ctaaaaaaac ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc
    86701 ttgcagtcaa ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt
    86761 tagaaatcat ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat
    86821 tgttgacttt cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat
    86881 ttggtgtcat attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa
    86941 aaaaacgtta attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca
    87001 acaaaaaacg aaagatgata gagaagactc attctttcgt ttataaagga tatatatata
    87061 cattgggagg gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat
    87121 aaagagtttg ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta
    87181 aacgatattc atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg
    87241 tgtaatcaga tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac
    87301 cttgatactt tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa
    87361 aacttgagag aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat
    87421 cactccttta tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc
    87481 gaataaaagc taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa
    87541 ttatgatagg atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa
    87601 atagcttttt ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga
    87661 gaaataatta ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc
    87721 gctgaagtgg ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt
    87781 gctgtaaaaa agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca
    87841 attgatggta ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta
    87901 tacaagcgtg aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc
    87961 ggacaaaaag catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct
    88021 tttcctgatg cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca
    88081 caagatggtt atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca
    88141 acgatccatg aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc
    88201 ggagttcaaa tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag
    88261 gccattccat ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa
    88321 ataatgatca acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca
    88381 gctttaagca caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca
    88441 caggaatttg gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt
    88501 ttcaatatta ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt
    88561 caagctaagc aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg
    88621 acgccaaaag aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt
    88681 gaagcagcaa gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta
    88741 aaagacgaga taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa
    88801 gtattagatg gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg
    88861 gatttaggtg tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc
    88921 cattatttcc caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt
    88981 tatgagaatt ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca
    89041 gagatttatt ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca
    89101 caatttcatg atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat
    89161 ccaatggcag atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct
    89221 tggggattgt tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat
    89281 gatttaagaa aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga
    89341 acgaatttgt acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta
    89401 tctcaccttc tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag
    89461 tagcggtttc tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa
    89521 acgtggaacc aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta
    89581 ttcaagtagc agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt
    89641 tggtgggcgg agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct
    89701 tgattggtac accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc
    89761 aagtgaaaac aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa
    89821 tcattgatca aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga
    89881 cagcagatcg ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg
    89941 acgtgacagc agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga
    90001 tacgaaagaa agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga
    90061 ttttctttaa tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg
    90121 taccggtagg aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac
    90181 aattcaaaaa gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag
    90241 atattacagg tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt
    90301 tgcatcgttc gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac
    90361 aagataataa tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag
    90421 agattttttt atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc
    90481 ctgcagtaaa taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata
    90541 acaaagaaaa aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag
    90601 caaggcgcaa ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg
    90661 ccagaggagg ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa
    90721 ccaacaagga ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc
    90781 aagccgatat ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc
    90841 agtcgtttac tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc
    90901 tacgcaagtg acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca
    90961 gcaggcaaaa gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga
    91021 aactggtaca gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga
    91081 gatgctttct gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc
    91141 gcaactgctc tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca
    91201 agatagtccc ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca
    91261 ccgtttaccg ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg
    91321 tccgcttgga gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac
    91381 tgaatcttca gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt
    91441 gccgaatctt gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa
    91501 acaagaagcg aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt
    91561 catgctttat gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg
    91621 tgcaaaatct tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga
    91681 agaactttcc cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct
    91741 cgaccgattc atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt
    91801 tgtctggtca caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt
    91861 cacttctctt tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga
    91921 tgtggatgct tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga
    91981 tcttttgcca gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg
    92041 gttagaccgt atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac
    92101 attggatcgt gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa
    92161 aaattttaag agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga
    92221 acaagttgag attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct
    92281 aggagaaagt gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag
    92341 acagactgct tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa
    92401 agcttttaat atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata
    92461 tgtcaaaaac tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc
    92521 attaaacacc ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac
    92581 gatgacaagt accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt
    92641 agaagatatt tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa
    92701 agcggaaaag tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg
    92761 tgaaggagta gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt
    92821 agaactgatc cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt
    92881 attggaagaa tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat
    92941 cccatttttt tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat
    93001 agctgatgat gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga
    93061 tgtcttttct attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac
    93121 cgatttgtcg attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt
    93181 catgttagac ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt
    93241 ctcaaaaatt tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg
    93301 aaaatatcag gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga
    93361 aaataaaata aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc
    93421 gcactataaa aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc
    93481 atgcttcttg cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa
    93541 aaacaatcat gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc
    93601 aaaaagcagc agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg
    93661 cagataataa tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt
    93721 tcatgaaagc tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca
    93781 atatgcaaga ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa
    93841 tgaaataaat caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga
    93901 agccatcatg gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat
    93961 tgcggctgct aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga
    94021 atcacttata caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa
    94081 tcatatgcaa gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc
    94141 gtttactgat ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat
    94201 acaacttctt ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata
    94261 aggagaactc tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca
    94321 agttggatgg agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta
    94381 gttgctttgc gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct
    94441 gtaatgatca atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac
    94501 agtttagcag cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt
    94561 tttgtctgga acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat
    94621 aatcttgccc gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc
    94681 ttgattcaag gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct
    94741 tcaaatgtcg cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca
    94801 ctgtcagttg gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt
    94861 acagttatga tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac
    94921 attacgatca aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt
    94981 ttaccagcga cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta
    95041 gctgatgggc aactgatcat tgatttggtg caaaaatata ttgcagagcc attcctagga
    95101 ttatctcaag ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc
    95161 tttggtattc atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca
    95221 caactaatca atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc
    95281 gtgttagatg caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct
    95341 tttgcatggt ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc
    95401 tctaaacgtg cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat
    95461 atcaacgaac cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca
    95521 ttcttgattg ctccagtagt agcaacagca attggatgga cagctactta tcttggccta
    95581 gtcgctccgg tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta
    95641 gcaacaggag cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc
    95701 ttgatttggg caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa
    95761 caataagatg aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg
    95821 aggaggaact gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt
    95881 gttagaagga tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa
    95941 gctgttcttt gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact
    96001 aatcaaagca cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat
    96061 ggtttaaaaa gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg
    96121 ttctttgatc gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat
    96181 ggtcacaggt ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag
    96241 agaagaagca aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca
    96301 tcgttttttt acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc
    96361 aaatcagtaa ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat
    96421 aaccgggctc gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca
    96481 tcgacaacga agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc
    96541 tgtgaggttc attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat
    96601 atgcttttag gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat
    96661 atgatcgaag caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga
    96721 agtaagctag aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct
    96781 tttttgtagg aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga
    96841 ttgaggaaat gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc
    96901 gagaacgttt tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg
    96961 atcggctaac aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt
    97021 acctaatggc cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga
    97081 tcaatcagat catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt
    97141 atctggcgcc attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata
    97201 aaatcagtta tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa
    97261 taaagcgtat ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc
    97321 cggaaaacca acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc
    97381 aaaaaggcaa ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata
    97441 tcgtttatca gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg
    97501 atgctttttg cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg
    97561 aatattcatg cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt
    97621 ctgtaatgat cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa
    97681 attatccatt taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg
    97741 cgaaatggaa tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa
    97801 aagtagaaca aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat
    97861 tattagggta tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag
    97921 aatatgaaga aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt
    97981 tttgataaaa aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc
    98041 atttgaagga aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc
    98101 ttttgtttta gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg
    98161 atttttttta gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat
    98221 tggaaaggac ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta
    98281 gtggaaacgt tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa
    98341 aaacaaacaa gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca
    98401 atcgcctcct aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc
    98461 tgtatccttt gtggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt
    98521 tatcattttt atataaacta tcgaatacag cattgatttt ttctttatcc tgatcttcaa
    98581 gctctcgaac aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaacc
    98641 gttttgatac gctaagtata tttactcctt gataaagtaa aatagatgca tgtgtatgcc
    98701 ttagtccatg caaagtaaat tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat
    98761 atttgctgac ggcatttgat gaaactaatc cattttttaa gttgaaaaaa acaaaattat
    98821 gagggttttt aacttcaaaa ttctcgaata attctttttg attgatttta aactttttta
    98881 agagatcaat cgtatgttta tcaattgaaa ttttccgatt tgaagtctcg ttttttgttt
    98941 ttccccattc atttaattta taattccatg ttttattaat agtgatgatt tgatcttcga
    99001 aatcgatatc ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca gtagcaccag
    99061 caactaacac gagcatagga gaagagtact gtgcagaaag gttttcttct acgagtttca
    99121 ttaaagattt aaattgatca taatctaaat atttatcttc ttttggcttc aatgaatctt
    99181 ttcctttgat tactgctttt ctagttggat caaacggaat taatccttct tctacggcat
    99241 ctttcaacga tgctctaata tggttattaa acttaataac ggtggatttt acatggtctt
    99301 tcgcatactt atttaaaaag cgttgatatc caactctatc taaatcagaa atcaatactg
    99361 ctggcatata tttctttata ttcattaacg tatcttcgta ttttctataa gtgataggag
    99421 atactgttcc ttctttgtag agttgcatcc aatcttcaaa gtaatctgat aataatagat
    99481 ttttgcgatc ccctttaaga cctttattca ggttatattc taattcgttg gcggcatctt
    99541 ttgcttcgcc ttttgtcttg aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt
    99601 ttttgtatga aatttcgtat tgccaactat ttccacgttt gactaatctt gccataattg
    99661 aatctactct ctttctctga tacaataggc actgtaaaga agcctattgt gtaggtttgt
    99721 ttttaacgcg tccacaaact ttggcgaggg agggggcgtg ttttttattt atactctgtt
    99781 gttcctgctt cgccaatcgg ctcagctgta aaatcatcag taaattgttt agacgcatta
    99841 agtttaaatg tacttaaatt tggtttattt ttagaaagta aatagaaaac cccagatgtt
    99901 gtcgtttcag gttgaactaa agaattagaa ttttcgtcat ataagaaata ttctccagct
    99961 gttctactat attccatacc gtctccatct attaaagatg cctgatacga agacaaagag
   100021 aaaggtgatg ttgctttatt ttttaacttg taagaaatag tagtacgata gtatttttct
   100081 ggaaaatttt ctattccact aaagttatat tcggcatttg taaaattatc ttcgtcattt
   100141 gttacctgtt cagtttttat agaagaaatt gtatattcta ggataccaac aactggcaag
   100201 gttttttcct tagaagcatt gtcgatagat tcgctatttt cttcagtgtc atttgactgc
   100261 gatgctattt gttttttaag ctcttcattt tcttttttta aactattgat tgttttttga
   100321 gtttcactat tagttgcatt atttgaaggc gaactacatg cacccaaagt taaaatacta
   100381 gtacaaataa atcctaatat taagtttttt ttcattttta attcctcact tctatgatat
   100441 tatttttatg tagaatctta gaaatgagat tttagtccgt gttgcagcac gggctttttt
   100501 ctttataact ttttagaggt tataggcaag atagtgggac acaaaaatat attattgtat
   100561 tccgtattta gaaaatccta attgaacttc accggaagtt ttttgctgtg ttgtacgcaa
   100621 tgcttcttct tcagacattc cattctgtac tttccatgca acaggcgaca tcccgtattt
   100681 gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgt tgagcgcttg tttgttgggc
   100741 ttctgtattt tgttgagatg cagcttgttg ttctttctga tcttgactga taatattgcc
   100801 agcatcatct gtagtcaatc cattttcata aagggccacg ccgaaagctt cccactcttt
   100861 gttggaccaa tttgcacgat cagctggagt tgactgtaaa gtgcgttgtt tcatctgttc
   100921 atatgtttct tcttgaggtg cggtttggat tgtatcctga ctggagctta taactgttgg
   100981 gctaggtttc gccgtagctt ggttggagct tgtaactgtt gaactagaat ctgttttaga
   101041 tgtagatttg ctagtagatg aactgatttc agttgtttct tttgttttac ttatatttgt
   101101 ttcttggtta gaagtggcat ctgttgattc agctttttta ttatttgaac aagctgaaag
   101161 tagcagagca gtacttaaca acaacataac gctaactttt ttcattttat aattcctctt
   101221 tctcgttgta atatgtgtgc taacacgggc ttttttatat aagaaaacga taagcgcttt
   101281 ctggaagacc gtaaagattc tttaattcct cgattttttt aggatattga tcattatctt
   101341 ctttataaag agaaacaatg agattagcag caaagcaatt agcttcgctt tcagatttgc
   101401 ttctagatgt tcttgttgat acataatagc tggataagcc acgatgaaaa atagcgtgac
   101461 ctaattcgtg agcgcaaatg tagaatcttt cctcagagtc tcgcagttca tcatttaaaa
   101521 agattatcgc acgatctcta atttcttgaa actgcccctt gggattctcg ataaaaggaa
   101581 cgtattgaat tttaatgccc atcttttcac aaatataaaa aggattagcg gactggtatt
   101641 tccgcttcaa ctcctcgact aaattaatcg tatccatctc cataagctca catctttttg
   101701 cctttttctt tgtcttcttt cacaatatcc cagaaagtcg ctatcaggat atcttttacg
   101761 cgctgtattt gttcgggtgt caatgtttcc ccaccataag acatattaac atttgagtct
   101821 agtagtttat caagttcaac cacttcctct tttgtagccc atttgggaac attattattt
   101881 cccaataaat aatcagttgt gacaccaaaa taatcagcaa ctttctttaa gttctcagat
   101941 tttggcgagg ctttatccca tcttcttatt tgtccattag agatgcccac ctgtctttct
   102001 acttctgcta tagtcacatg cttttcgtct gctaattctt taatcttagt aactaaactc
   102061 attattatca acctttcaaa gctgaaaaga aaataaatag cttaaaagtt atatttttgg
   102121 ttgacaatta gcttttaagc tagtatattt aattcgtaag ctaaattgtt agctaaataa
   102181 gagcaacaaa aaactctact aatttaaaac attctctcgg tcgccaaact tagaaatgtt
   102241 attttagagg ctttttataa gtcttattta actatgtatt cataatagct taaaagctaa
   102301 taggtgtcaa cgatttagct aattttttag cttacaaatt attttttaga aaggagctat
   102361 ttttatgtct gagaatttag acttaaaaat tcgagcggag atgagaaaaa gaagaatgac
   102421 tttcaaagaa ctagctgcgc ttgttggtat ttcaggagct tatttatcag atatcctaaa
   102481 cggcaatcgt gatggtaaaa aagctcaaga acacatagaa actgtaaaaa aaatattagg
   102541 tattcaatag gaggaacagc taatgcaata tctagaagcg aaaatcccag ttccagaagg
   102601 ctatgtaatt atctctcaag tggattacga ggagttaaaa aaagctgatg atactggtag
   102661 atggatgacg ttgccagaag tgctagaacg gattaacaga aaatatgatt ggtttacttc
   102721 tagagtttta aagaacccaa gatatagaaa tattatcgat atcgagaaaa acaaaaatgg
   102781 atttgtctat tatccagttg aaggaagaga cacatatcta tttttaagaa gtaaaacact
   102841 tgaattttta gaaacaaatt tttcggaaat cttaaggagg caagcggatg ggaaaattta
   102901 atagagcgct agt
//
LOCUS       DAFCNJ010000003        95905 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000003 DAFCNJ010000000
VERSION     DAFCNJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 95905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.003"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..562
                     /locus_tag="LNE35_000271"
     CDS             <1..562
                     /locus_tag="LNE35_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK6909460.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="LNE35_000272"
     CDS             762..1076
                     /locus_tag="LNE35_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6909461.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="LNE35_000273"
     CDS             1097..1474
                     /locus_tag="LNE35_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6909462.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="LNE35_000274"
     CDS             1484..2257
                     /locus_tag="LNE35_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909463.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="LNE35_000275"
     CDS             2279..3682
                     /locus_tag="LNE35_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK6909464.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="LNE35_000276"
     CDS             3864..5048
                     /locus_tag="LNE35_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6909465.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="LNE35_000277"
     CDS             5045..5335
                     /locus_tag="LNE35_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909466.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="LNE35_000278"
     CDS             5332..5553
                     /locus_tag="LNE35_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909467.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="LNE35_000279"
     CDS             5595..6374
                     /locus_tag="LNE35_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBK6909468.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="LNE35_000280"
     CDS             6441..6935
                     /locus_tag="LNE35_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK6909469.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="LNE35_000281"
     CDS             6991..7629
                     /locus_tag="LNE35_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909470.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="LNE35_000282"
     CDS             7701..8096
                     /locus_tag="LNE35_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6909471.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="LNE35_000283"
     CDS             8080..10533
                     /locus_tag="LNE35_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6909472.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="LNE35_000284"
     CDS             10530..12557
                     /locus_tag="LNE35_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909473.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="LNE35_000285"
     CDS             12554..13576
                     /locus_tag="LNE35_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK6909474.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="LNE35_000286"
     CDS             13593..14522
                     /locus_tag="LNE35_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6909475.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="LNE35_000287"
     CDS             14767..14883
                     /locus_tag="LNE35_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK6909476.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..15702
                     /locus_tag="LNE35_000288"
     CDS             14899..15702
                     /locus_tag="LNE35_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK6909477.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15937..16173
                     /locus_tag="LNE35_000289"
     CDS             15937..16173
                     /locus_tag="LNE35_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909478.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="LNE35_000290"
     CDS             16180..18132
                     /locus_tag="LNE35_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBK6909479.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="LNE35_000291"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="LNE35_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBK6909480.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19063..20103
                     /locus_tag="LNE35_000292"
     CDS             19063..20103
                     /locus_tag="LNE35_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK6909481.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20201..20389
                     /locus_tag="LNE35_000293"
     CDS             20201..20389
                     /locus_tag="LNE35_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBK6909482.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20447..20800)
                     /locus_tag="LNE35_000294"
     CDS             complement(20447..20800)
                     /locus_tag="LNE35_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909483.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21331..21753
                     /locus_tag="LNE35_000295"
     CDS             21331..21753
                     /locus_tag="LNE35_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6909484.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21750..21980
                     /locus_tag="LNE35_000296"
     CDS             21750..21980
                     /locus_tag="LNE35_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909485.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22478..22678
                     /locus_tag="LNE35_000297"
     CDS             22478..22678
                     /locus_tag="LNE35_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBK6909486.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22705..23898
                     /locus_tag="LNE35_000298"
     CDS             22705..23898
                     /locus_tag="LNE35_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6909487.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="LNE35_000299"
     CDS             complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="LNE35_000299"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBK6909488.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25590..26513)
                     /locus_tag="LNE35_000300"
     CDS             complement(25590..26513)
                     /locus_tag="LNE35_000300"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBK6909489.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26709..27314
                     /locus_tag="LNE35_000301"
     CDS             26709..27314
                     /locus_tag="LNE35_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6909490.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27622..28638
                     /locus_tag="LNE35_000302"
     CDS             27622..28638
                     /locus_tag="LNE35_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK6909491.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28779..30080
                     /locus_tag="LNE35_000303"
     CDS             28779..30080
                     /locus_tag="LNE35_000303"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBK6909492.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30126..30788
                     /gene="deoC"
                     /locus_tag="LNE35_000304"
     CDS             30126..30788
                     /gene="deoC"
                     /locus_tag="LNE35_000304"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBK6909493.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30809..31207
                     /locus_tag="LNE35_000305"
     CDS             30809..31207
                     /locus_tag="LNE35_000305"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBK6909494.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31273..32355
                     /locus_tag="LNE35_000306"
     CDS             31273..32355
                     /locus_tag="LNE35_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6909495.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32567..34129
                     /locus_tag="LNE35_000307"
     CDS             32567..34129
                     /locus_tag="LNE35_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909496.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34122..35255
                     /locus_tag="LNE35_000308"
     CDS             34122..35255
                     /locus_tag="LNE35_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909497.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35255..36211
                     /locus_tag="LNE35_000309"
     CDS             35255..36211
                     /locus_tag="LNE35_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909498.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36310..37476
                     /gene="deoB"
                     /locus_tag="LNE35_000310"
     CDS             36310..37476
                     /gene="deoB"
                     /locus_tag="LNE35_000310"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK6909499.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37500..38318
                     /locus_tag="LNE35_000311"
     CDS             37500..38318
                     /locus_tag="LNE35_000311"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK6909500.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38335..39042
                     /gene="deoD"
                     /locus_tag="LNE35_000312"
     CDS             38335..39042
                     /gene="deoD"
                     /locus_tag="LNE35_000312"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK6909501.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="LNE35_000313"
     CDS             complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="LNE35_000313"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK6909502.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40198..40779)
                     /locus_tag="LNE35_000314"
     CDS             complement(40198..40779)
                     /locus_tag="LNE35_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6909503.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="LNE35_000315"
     CDS             complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="LNE35_000315"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBK6909504.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41914..42327
                     /gene="rpsL"
                     /locus_tag="LNE35_000316"
     CDS             41914..42327
                     /gene="rpsL"
                     /locus_tag="LNE35_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBK6909505.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42411..42881
                     /gene="rpsG"
                     /locus_tag="LNE35_000317"
     CDS             42411..42881
                     /gene="rpsG"
                     /locus_tag="LNE35_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBK6909506.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42975..45062
                     /gene="fusA"
                     /locus_tag="LNE35_000318"
     CDS             42975..45062
                     /gene="fusA"
                     /locus_tag="LNE35_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK6909507.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45234..46421
                     /gene="tuf"
                     /locus_tag="LNE35_000319"
     CDS             45234..46421
                     /gene="tuf"
                     /locus_tag="LNE35_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK6909508.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46828..47136
                     /gene="rpsJ"
                     /locus_tag="LNE35_000320"
     CDS             46828..47136
                     /gene="rpsJ"
                     /locus_tag="LNE35_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBK6909509.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            47167..47796
                     /gene="rplC"
                     /locus_tag="LNE35_000321"
     CDS             47167..47796
                     /gene="rplC"
                     /locus_tag="LNE35_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBK6909510.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47824..48447
                     /gene="rplD"
                     /locus_tag="LNE35_000322"
     CDS             47824..48447
                     /gene="rplD"
                     /locus_tag="LNE35_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBK6909511.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48447..48737
                     /gene="rplW"
                     /locus_tag="LNE35_000323"
     CDS             48447..48737
                     /gene="rplW"
                     /locus_tag="LNE35_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBK6909512.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48776..49609
                     /gene="rplB"
                     /locus_tag="LNE35_000324"
     CDS             48776..49609
                     /gene="rplB"
                     /locus_tag="LNE35_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBK6909513.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49652..49930
                     /gene="rpsS"
                     /locus_tag="LNE35_000325"
     CDS             49652..49930
                     /gene="rpsS"
                     /locus_tag="LNE35_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBK6909514.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49953..50300
                     /gene="rplV"
                     /locus_tag="LNE35_000326"
     CDS             49953..50300
                     /gene="rplV"
                     /locus_tag="LNE35_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBK6909515.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50314..50970
                     /gene="rpsC"
                     /locus_tag="LNE35_000327"
     CDS             50314..50970
                     /gene="rpsC"
                     /locus_tag="LNE35_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBK6909516.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50973..51407
                     /gene="rplP"
                     /locus_tag="LNE35_000328"
     CDS             50973..51407
                     /gene="rplP"
                     /locus_tag="LNE35_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBK6909517.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51397..51585
                     /gene="rpmC"
                     /locus_tag="LNE35_000329"
     CDS             51397..51585
                     /gene="rpmC"
                     /locus_tag="LNE35_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBK6909518.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51610..51876
                     /gene="rpsQ"
                     /locus_tag="LNE35_000330"
     CDS             51610..51876
                     /gene="rpsQ"
                     /locus_tag="LNE35_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBK6909519.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51934..52302
                     /gene="rplN"
                     /locus_tag="LNE35_000331"
     CDS             51934..52302
                     /gene="rplN"
                     /locus_tag="LNE35_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBK6909520.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52339..52647
                     /gene="rplX"
                     /locus_tag="LNE35_000332"
     CDS             52339..52647
                     /gene="rplX"
                     /locus_tag="LNE35_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBK6909521.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52675..53214
                     /gene="rplE"
                     /locus_tag="LNE35_000333"
     CDS             52675..53214
                     /gene="rplE"
                     /locus_tag="LNE35_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBK6909522.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            53233..53418
                     /locus_tag="LNE35_000334"
     CDS             53233..53418
                     /locus_tag="LNE35_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBK6909523.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53454..53852
                     /gene="rpsH"
                     /locus_tag="LNE35_000335"
     CDS             53454..53852
                     /gene="rpsH"
                     /locus_tag="LNE35_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBK6909524.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53884..54420
                     /gene="rplF"
                     /locus_tag="LNE35_000336"
     CDS             53884..54420
                     /gene="rplF"
                     /locus_tag="LNE35_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBK6909525.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54581..54937
                     /gene="rplR"
                     /locus_tag="LNE35_000337"
     CDS             54581..54937
                     /gene="rplR"
                     /locus_tag="LNE35_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBK6909526.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54958..55458
                     /gene="rpsE"
                     /locus_tag="LNE35_000338"
     CDS             54958..55458
                     /gene="rpsE"
                     /locus_tag="LNE35_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBK6909527.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55473..55652
                     /gene="rpmD"
                     /locus_tag="LNE35_000339"
     CDS             55473..55652
                     /gene="rpmD"
                     /locus_tag="LNE35_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBK6909528.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55696..56136
                     /gene="rplO"
                     /locus_tag="LNE35_000340"
     CDS             55696..56136
                     /gene="rplO"
                     /locus_tag="LNE35_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBK6909529.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            56136..57431
                     /gene="secY"
                     /locus_tag="LNE35_000341"
     CDS             56136..57431
                     /gene="secY"
                     /locus_tag="LNE35_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBK6909530.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57493..58140
                     /locus_tag="LNE35_000342"
     CDS             57493..58140
                     /locus_tag="LNE35_000342"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBK6909531.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58331..58549
                     /gene="infA"
                     /locus_tag="LNE35_000343"
     CDS             58331..58549
                     /gene="infA"
                     /locus_tag="LNE35_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBK6909532.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58582..58698
                     /gene="rpmJ"
                     /locus_tag="LNE35_000344"
     CDS             58582..58698
                     /gene="rpmJ"
                     /locus_tag="LNE35_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBK6909533.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58716..59081
                     /gene="rpsM"
                     /locus_tag="LNE35_000345"
     CDS             58716..59081
                     /gene="rpsM"
                     /locus_tag="LNE35_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBK6909534.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            59109..59498
                     /gene="rpsK"
                     /locus_tag="LNE35_000346"
     CDS             59109..59498
                     /gene="rpsK"
                     /locus_tag="LNE35_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBK6909535.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59578..60516
                     /locus_tag="LNE35_000347"
     CDS             59578..60516
                     /locus_tag="LNE35_000347"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBK6909536.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60544..60924
                     /gene="rplQ"
                     /locus_tag="LNE35_000348"
     CDS             60544..60924
                     /gene="rplQ"
                     /locus_tag="LNE35_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBK6909537.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            61196..62035
                     /locus_tag="LNE35_000349"
     CDS             61196..62035
                     /locus_tag="LNE35_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6909538.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            62011..62880
                     /locus_tag="LNE35_000350"
     CDS             62011..62880
                     /locus_tag="LNE35_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6909539.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62870..63667
                     /locus_tag="LNE35_000351"
     CDS             62870..63667
                     /locus_tag="LNE35_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK6909540.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63870..64616
                     /gene="truA"
                     /locus_tag="LNE35_000352"
     CDS             63870..64616
                     /gene="truA"
                     /locus_tag="LNE35_000352"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK6909541.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64949..67618)
                     /locus_tag="LNE35_000353"
     CDS             complement(64949..67618)
                     /locus_tag="LNE35_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBK6909542.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67856..68044)
                     /locus_tag="LNE35_000354"
     CDS             complement(67856..68044)
                     /locus_tag="LNE35_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBK6909543.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            68314..68676
                     /locus_tag="LNE35_000355"
     CDS             68314..68676
                     /locus_tag="LNE35_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6909544.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68728..70410
                     /locus_tag="LNE35_000356"
     CDS             68728..70410
                     /locus_tag="LNE35_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBK6909545.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            70527..72563
                     /gene="recG"
                     /locus_tag="LNE35_000357"
     CDS             70527..72563
                     /gene="recG"
                     /locus_tag="LNE35_000357"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBK6909546.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72612..73613
                     /gene="plsX"
                     /locus_tag="LNE35_000358"
     CDS             72612..73613
                     /gene="plsX"
                     /locus_tag="LNE35_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBK6909547.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73735..73977
                     /gene="acpP"
                     /locus_tag="LNE35_000359"
     CDS             73735..73977
                     /gene="acpP"
                     /locus_tag="LNE35_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK6909548.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            74245..75249
                     /locus_tag="LNE35_000360"
     CDS             74245..75249
                     /locus_tag="LNE35_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909549.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            75250..76194
                     /locus_tag="LNE35_000361"
     CDS             75250..76194
                     /locus_tag="LNE35_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909550.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            76194..77156
                     /locus_tag="LNE35_000362"
     CDS             76194..77156
                     /locus_tag="LNE35_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909551.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            77183..78097
                     /locus_tag="LNE35_000363"
     CDS             77183..78097
                     /locus_tag="LNE35_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909552.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            78123..79904
                     /locus_tag="LNE35_000364"
     CDS             78123..79904
                     /locus_tag="LNE35_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6909553.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            80252..80938
                     /locus_tag="LNE35_000365"
     CDS             80252..80938
                     /locus_tag="LNE35_000365"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBK6909554.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            80958..84539
                     /gene="smc"
                     /locus_tag="LNE35_000366"
     CDS             80958..84539
                     /gene="smc"
                     /locus_tag="LNE35_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBK6909555.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            84548..85357
                     /locus_tag="LNE35_000367"
     CDS             84548..85357
                     /locus_tag="LNE35_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6909556.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            85368..86366
                     /gene="ftsY"
                     /locus_tag="LNE35_000368"
     CDS             85368..86366
                     /gene="ftsY"
                     /locus_tag="LNE35_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBK6909557.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            86553..88820
                     /gene="gshAB"
                     /locus_tag="LNE35_000369"
     CDS             86553..88820
                     /gene="gshAB"
                     /locus_tag="LNE35_000369"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBK6909558.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(88909..89361)
                     /locus_tag="LNE35_000370"
     CDS             complement(88909..89361)
                     /locus_tag="LNE35_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBK6909559.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            89511..90461
                     /locus_tag="LNE35_000371"
     CDS             89511..90461
                     /locus_tag="LNE35_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK6909560.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            90454..91413
                     /locus_tag="LNE35_000372"
     CDS             90454..91413
                     /locus_tag="LNE35_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK6909561.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            91410..92165
                     /locus_tag="LNE35_000373"
     CDS             91410..92165
                     /locus_tag="LNE35_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6909562.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            92188..93141
                     /locus_tag="LNE35_000374"
     CDS             92188..93141
                     /locus_tag="LNE35_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6909563.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(93785..94081)
                     /locus_tag="LNE35_000375"
     CDS             complement(93785..94081)
                     /locus_tag="LNE35_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBK6909564.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            94436..94792
                     /locus_tag="LNE35_000376"
     CDS             94436..94792
                     /locus_tag="LNE35_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6909565.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            94802..95701
                     /locus_tag="LNE35_000377"
     CDS             94802..95701
                     /locus_tag="LNE35_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBK6909566.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        31584 a        17030 c        20163 g        27128 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18961 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    19021 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    19081 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    19141 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    19201 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    19261 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
    19321 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
    19381 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
    19441 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
    19501 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
    19561 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
    19621 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
    19681 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
    19741 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
    19801 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
    19861 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
    19921 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
    19981 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
    20041 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
    20101 tagtgcgttt tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt
    20161 taatatagat ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat
    20221 attgtgtcct atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa
    20281 aaatttccct ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat
    20341 gaatataata acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg
    20401 aaactctcat cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat
    20461 ttcttgcttc attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa
    20521 agctatccat gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag
    20581 gtaagaaaaa ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg
    20641 tacttgggtg ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca
    20701 ttgttcccac ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac
    20761 caacggctct aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat
    20821 tttactgttc ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt
    20881 tcctatagaa cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg
    20941 ctgattttat ttacctatac aactttataa aatttagtca tactcctaga aagtagataa
    21001 aattggcgac ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct
    21061 cttaattaag ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa
    21121 tgtggttttg cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct
    21181 gtttatgtgc ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca
    21241 acaaatcccc cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact
    21301 ccctttcacg acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata
    21361 gaaaatcagt ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc
    21421 aaacatctag acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt
    21481 gtcaactctt tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt
    21541 ggattaagaa tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag
    21601 aaaaaacgga acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg
    21661 gagttactaa atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg
    21721 attaaagaat tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc
    21781 ccctcattgt aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt
    21841 atcgagggta tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc
    21901 atatggttat tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt
    21961 tatcgttcga aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc
    22021 ttcccgattt gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga
    22081 taggctaaca agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt
    22141 aagaaaagag ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt
    22201 agtaattcga aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga
    22261 tactcccgta tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca
    22321 tgagaaccat gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga
    22381 tattcttctc ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt
    22441 ggagttttcc tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga
    22501 tttagttgag ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga
    22561 attgatgatt acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg
    22621 tgaagctgtt gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt
    22681 tttgcacagt aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga
    22741 acctattatt ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat
    22801 cgtagtggat tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg
    22861 aaaccagaag aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa
    22921 attttccttc catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca
    22981 actattaaga agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt
    23041 gatgtgcaaa attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct
    23101 gttcaaggac tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca
    23161 aatccatcta agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca
    23221 aaagaagaat ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt
    23281 ttatttatct ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc
    23341 attcaatggg aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat
    23401 tacaagaatc aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat
    23461 attgtattag ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat
    23521 ctaattcaga cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta
    23581 tcatatgcta ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg
    23641 ttacggcatt ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa
    23701 gatcgtctag gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct
    23761 aacagtaatt tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa
    23821 caaaattatg atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa
    23881 atgaaaaaag tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat
    23941 aagaggattt ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag
    24001 tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt
    24061 tttgcaagac ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta
    24121 cagcacggat accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc
    24181 ggatccctgg aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga
    24241 tttcttcacg caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt
    24301 cgccaagaac acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat
    24361 caggcatgcc taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg
    24421 gttcgatcaa ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga
    24481 tcgtctgtgc tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta
    24541 ggaaagagac gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga
    24601 attcattccc aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta
    24661 agaagcgatc tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt
    24721 ccattacttg ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca
    24781 attggtcacc gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg
    24841 aaagaccaag caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat
    24901 cgataaagtt atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc
    24961 gcagcaattc gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa
    25021 aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt
    25081 caaagccttc aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt
    25141 ttggtgtttc tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat
    25201 attcgcggtt tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac
    25261 catagcaaat ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg
    25321 cattctcatc atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg
    25381 cttttacttc ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac
    25441 gaatacgtct tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt
    25501 caacgttcgt catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta
    25561 tctcaaaata gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg
    25621 atatgatttt cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat
    25681 tcctgtaaat ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct
    25741 tcacgatctc cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc
    25801 agtaacgcac caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca
    25861 tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc
    25921 gtattgatcc cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga
    25981 atgatatcat acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt
    26041 acacggttca agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg
    26101 tccatgattc cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc
    26161 agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt
    26221 gtactttttc caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg
    26281 tatttatgaa gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga
    26341 taaatcagat gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca
    26401 ttcaaacttt tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa
    26461 aattctcgcc attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc
    26521 tcctgcgttc tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact
    26581 taagagagat tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt
    26641 cttgacttgc gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga
    26701 tggaagacat gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac
    26761 aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt
    26821 cacgtgaaac agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt
    26881 tacctgatga aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt
    26941 tggcatttgc gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc
    27001 tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata
    27061 tctatgaagc agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg
    27121 ctggaaaaaa agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg
    27181 gtacactgac aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg
    27241 aagaaacatt tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa
    27301 gtgtgaaaga ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc
    27361 atgtacgggt acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt
    27421 ttttcttcaa aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag
    27481 atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa
    27541 catactttcc ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt
    27601 agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc
    27661 gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag
    27721 acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct
    27781 tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat
    27841 ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg
    27901 atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt
    27961 tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat
    28021 gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat
    28081 cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg
    28141 ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat
    28201 caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt
    28261 aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa
    28321 aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc
    28381 ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata
    28441 ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag
    28501 ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct
    28561 caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa
    28621 gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt
    28681 tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat
    28741 aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg
    28801 aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact
    28861 atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc
    28921 aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg
    28981 tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg
    29041 gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta
    29101 aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc
    29161 ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag
    29221 tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc
    29281 gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa
    29341 aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca
    29401 tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg
    29461 ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg
    29521 gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc
    29581 tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga
    29641 caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa
    29701 aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac
    29761 gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa
    29821 aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca
    29881 aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg
    29941 tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca
    30001 ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc
    30061 atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa
    30121 aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag
    30181 aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca
    30241 atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta
    30301 cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag
    30361 atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat
    30421 caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc
    30481 gtgcgcttgt caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag
    30541 cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa
    30601 caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg
    30661 gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg
    30721 gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag
    30781 gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac
    30841 agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg
    30901 tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg
    30961 attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac
    31021 atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg
    31081 tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa
    31141 tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga
    31201 tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga
    31261 ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca
    31321 gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc
    31381 tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca
    31441 ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga
    31501 ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt
    31561 gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca
    31621 gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc
    31681 atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt
    31741 gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta
    31801 aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg
    31861 aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca
    31921 gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat
    31981 gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc
    32041 gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca
    32101 ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta
    32161 gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt
    32221 tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa
    32281 aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact
    32341 cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg
    32401 gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg
    32461 gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt
    32521 agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa
    32581 tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa
    32641 catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg
    32701 caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt
    32761 gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg
    32821 gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt
    32881 gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca
    32941 aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc
    33001 agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact
    33061 gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat
    33121 catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga
    33181 aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt
    33241 caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc
    33301 attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct
    33361 agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc
    33421 tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc
    33481 tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac
    33541 taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca
    33601 taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca
    33661 tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag
    33721 agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt
    33781 atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct
    33841 gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa
    33901 acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga
    33961 agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat
    34021 cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt
    34081 agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa
    34141 aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga
    34201 tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag
    34261 cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta
    34321 cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac
    34381 aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa
    34441 aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg
    34501 ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga
    34561 tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct
    34621 atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat
    34681 ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg
    34741 cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg
    34801 ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt
    34861 tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa
    34921 cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg
    34981 tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta
    35041 ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca
    35101 atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca
    35161 tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac
    35221 caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt
    35281 gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac
    35341 tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc
    35401 ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg
    35461 gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc
    35521 aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc
    35581 tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac
    35641 gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat
    35701 tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg
    35761 ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca
    35821 agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc
    35881 tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc
    35941 agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg
    36001 gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat
    36061 cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc
    36121 atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc
    36181 aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac
    36241 gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa
    36301 ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa
    36361 gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa
    36421 gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt
    36481 gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct
    36541 gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca
    36601 tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct
    36661 ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt
    36721 gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag
    36781 atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt
    36841 cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg
    36901 ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc
    36961 ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag
    37021 atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg
    37081 gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc
    37141 aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat
    37201 gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac
    37261 ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg
    37321 cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct
    37381 caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact
    37441 gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa
    37501 tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa
    37561 aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg
    37621 aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg
    37681 ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag
    37741 ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga
    37801 tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat
    37861 catttgtacc aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc
    37921 ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt
    37981 atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta
    38041 aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa
    38101 tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag
    38161 cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta
    38221 tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt
    38281 ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt
    38341 gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct
    38401 ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta
    38461 cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca
    38521 ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta
    38581 aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat
    38641 ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc
    38701 tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa
    38761 gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat
    38821 gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca
    38881 gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt
    38941 agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat
    39001 gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct
    39061 gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg
    39121 ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct
    39181 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    39241 tattgtgact tgtgaccgag aagttggcag gagaaaaccg tttatacgaa aaaagggact
    39301 gtgacaaatc gtgtcacagt ccctttttta tagtgttttt tataggtagt attttttagc
    39361 aactgttaag tcatcattta attcataaac aagtggttga cctgttggaa tttcaagatc
    39421 catgatgtct tcgtcagaga taccttcgat atgtttagct agggcgcgta atgagttacc
    39481 atgtgcagct actaaaacag ttttgttgtc tagtaatgct ggagcaattt catcttgcca
    39541 gaatggcaaa gcgcgttcta atgtcacttt caagttttct ccaccaggaa tatcgcgttt
    39601 gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt cttccattaa
    39661 tggaggaaga gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc cgtatttttc
    39721 agctgtttct tttttgttca aaccttgtaa tttaccatag tgacgttcgt ttaagcgcca
    39781 tgatttgatt tgtggtaccc aaagttgatc tgaatattct aagatcaagt tacatgtttt
    39841 gattgcacga gttaaaacag aagtgtaagc aacatcaaat tcgattccag cttctttgat
    39901 tttgcgtccg ccttcttttg cttcttcgat tccttctggt gctagattta catcagccca
    39961 accagtaaat tggttcaatg cattccattc actaagaccg tgacgagaaa aaactaattt
    40021 tggcatgttt agctctcctt ttaagatagg tttatatttc tgaaattttc agttctgttt
    40081 tattgtacaa ttttttggaa gagatttcca tcgaaaaatt agataagatt ctatgagaaa
    40141 acaagtagag aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt ttaggtatta
    40201 gatgcataat tgctcgattt ctttttcagg taaattgata tatttataac atgttccaac
    40261 tgaaacaccg cattttgaag aaataaattg gatcgtttct tttttttcat aatagagacg
    40321 tcggattttt ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt ttcctttttt
    40381 acgagcttca tctaatccaa ctaatgtacg ttctttgatc aatgcgcact ccatgtttgc
    40441 taggttttcc attaattgga aatagatttt tcccatttcg cgcgtatcga ttccttcatc
    40501 aagactaatc aagtgcagcc cgcgatcttt gaataattgc gtgagttctg ttaattgccg
    40561 cgtagatttg cctaatcgat gaagttggca aatgactagt gcatcgcctt gttgcatgtc
    40621 agcgatcact gactccaaat gaacagcagt agaatcattt tgttccactt catatgtctc
    40681 atggaaaatt tcttgacaac caaagtcaga taattgatca acctgagttg ttaaatcaac
    40741 gtcatttatg gttgtgcgtg cataaccaat aattttcatg gtgattcctt tctagcagtg
    40801 attttctaac tggatataac agtaaaaatg tagcataaag tacgtcaaaa agatagtttt
    40861 ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa gcagtttttc tttatttacg
    40921 acggattgcg tcaagaatct caacgccttt ttctgagccg acaactactt tagaaaccat
    40981 gtttaggaaa agcccatgtt ctactacccc aacaagttca tctaaatagg tagctaagct
    41041 ttctggctgt tcgatgactt ctagatgcag atcaatgatg taatgaccac catctgtggt
    41101 taagacttct ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt cttcaaaaat
    41161 atgaagtagc tgttgggagc cataagggat cacttcaact gggagtggga acttcccaag
    41221 tttatcgact aatttagaag aatcaacgat ccaaatcgtt tcttttgagt acgtagcgac
    41281 tattttctcg aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat cagcactgat
    41341 ttcatcagca ccgtctattg tcaaatcgac tacggggact tcatcaatac tctttaacgg
    41401 tattcctaat ttttccgctt gttcttttgt cgcatttgaa gtagtaactc ctgtgatacg
    41461 taaaccttct tcacgcatac ggcgaccaat ctcttctacc atataatagg cggttgaacc
    41521 ggtcccgaga cctacgatca tcccgtcctt tacgaatttc gcggcttcaa tgccagctaa
    41581 ttgttttaaa ttcatctttg cgtccacctt tttttagttt gagcatcgtg ctttcctcac
    41641 tattttagcg tgatttacgg taaaaagata ccattctttt ttattccaat aaagcttttt
    41701 caaaaaaaat tgcatctctt gttgacatgt tttttcattc gtgatattct aacaaaggtg
    41761 ctttatctac aggtttctgt gaagaaacgt gctgactgtt tatcaaagca tatttgataa
    41821 ggatgcagac attgcattta ttttttatca caaaaaaaga accacctgga tgtgtggaac
    41881 tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta caattaacca attagtacgt
    41941 aaacctcgta aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa aggatataac
    42001 agctttaaga aaactcaaac taacgtaaac tcacctcaaa aacgtggggt agcaactcgt
    42061 gtgggtacaa tgacacctaa aaaaccgaac tcagctttac gtaaatatgc acgtgttcgt
    42121 ttgtcaaact taattgaagt tactgcttat atcccaggga ttggtcataa cttacaagaa
    42181 cacagtgttg tattattacg cggtggtcgt gtaaaagact taccaggggt acgttaccat
    42241 atcgttcgcg gtgctttaga tactgctggt gttaacgacc gtaaacaaag ccgttctaaa
    42301 tacggaacta aaaaacctaa agcttaattt taattattaa acaaatgaaa cttgcttcta
    42361 ctatatagaa gtaattacta tgaacaatat ttcggaagga ggagttacgg atgccacgta
    42421 aaggtcctgt tgcaaaacgt gatgttttac cagatcctat ttataactca aaattagtaa
    42481 ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg gattgcagct aacattatct
    42541 ataatgcctt tgacttgatc aaagaatcta caggtaacga tccattagaa gtgtttgaac
    42601 aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt gggggttcaa
    42661 actaccaagt accagttgaa gttcgccctg aacgccgcgt tactctaggt cttcgttggg
    42721 tagtaaacta cgctcgcttg cgcggtgaac acacaatgga agaacgctta gcaaaagaaa
    42781 tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac actcataaaa
    42841 tggctgatgc caaccgtgcg tttgctcact atcgctggta agattctctc tgtttggtcg
    42901 gcttttataa gtcgacctca gatagtgtcc ttatttttag caactagata gtaaagagag
    42961 gagaaacaag gagaatgacc agagaatttt ctttacaaaa cactcgtaat atcggtatca
    43021 tggcccacat cgatgctggt aaaacgactg ctactgaacg tatcttgtac tatactggta
    43081 aaatccataa aattggtgaa actcatgaag gagcttcaca aatggactgg atggaacaag
    43141 aacaagaacg tggtattacg atcacttctg ctgctactac agcacaatgg aagggtcacc
    43201 gaatcaacat catcgacaca ccaggacacg tagacttcac agttgaagtt gaacgttcat
    43261 tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt gaacctcaaa
    43321 ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta tttgcaaaca
    43381 aaatggacaa gatcggtgct gacttcttat attcagtatc aactttacat gaccgtttgc
    43441 aagcaaacgc tcacccaatc caattaccaa ttggtgcgga agacgacttc acaggaatta
    43501 tcgaccttgt aacaatgaag gctgaaatgt atacaaacga tcttggaaca gagatcgaag
    43561 aaacagaaat tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa aaattggttg
    43621 aagctgtagc tgaaactgat gaagaactta ctttgaaata cttagaaggt gaagaaatca
    43681 ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa ttctacccag
    43741 tactatgtgg ttcagcattt aagaacaaag gggttcaatt actattggac gctgttcttg
    43801 attacttgcc atcaccgtta gatatcccag ctatcaaagg gattgaccct aaaactgatg
    43861 aagaagtaga acgtcctgca gacgacagcg ctcctttctc agctttagca tttaaagtta
    43921 tgactgaccc attcgtaggt cgtttaactt tcttccgtgt gtactctggt gttttacaat
    43981 caggttcata cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt cgtatcctac
    44041 agatgcatgc caactctcgt tcagaaatct ctgaagttta cgctggagat atcgctgctg
    44101 ctgttggttt gaaagacaca actactgggg acacactatg tgatgaaaag agtctagtta
    44161 ttcttgaatc catggaattc ccagaaccag ttatccaagt cgctattgaa cctaaatcaa
    44221 aagctgacca agataaaatg ggtgtggctt tacaaaaact atctgaagaa gatcctactt
    44281 tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg ggtgaacttc
    44341 acttagatat catcgttgac cgtatgcgtc gtgaattcaa agttgaagct aacgtaggtg
    44401 cgcctcaagt atcttaccgt gaaactttcc gtgctggcac tcaagccgaa ggtaagtttg
    44461 tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt acaccaaatg
    44521 aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt cctcgtgaat
    44581 acattccagc tgttgaaact ggtttgaaag atgctatgga aaatggtgtc ttagctggct
    44641 atccattagt agatatcaaa gctaaacttt acgatggttc ataccatgat gtcgattcaa
    44701 atgaaactgc cttccgtgtt gctgcttcta tggcattacg tgcagcagct aagaaagcta
    44761 atccagtaat ccttgaacca atcatggctg tagaagttgt tatccctgaa gattatttag
    44821 gtgatgttat gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg gaagctcgcg
    44881 ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt ggttatgcta
    44941 caactcttcg ttctgctact caaggtcgtg gtacatttac aatgacattt gaccactacg
    45001 aagatgtacc aaaatcagta caagaagaaa tcattaagaa aaacggcgga aaagctgaat
    45061 aatttcttaa aaccggatag tcctgcaaag agattatcca aagagactat atttttaata
    45121 tagtttcgtg taaaataatt atcgatgata tgggcatgtg gtgtaccttg tgtacatcat
    45181 atacctatca gacaaataaa aaatatattg tatttttagg aggacatttt aaaatggcaa
    45241 aagaaaaatt tgaccgttct aaaccacacg ttaacattgg tactatcgga cacgttgacc
    45301 atggtaaaac tacattaaca gctgcaatca caactgtact atctaagaaa aacggcggcc
    45361 aagctatggc ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc ggaatcacaa
    45421 tctcaacagc tcacgttgag tatgaaacag atacacgcca ctacgctcac gttgactgcc
    45481 caggacacgc ggattacgtt aaaaacatga tcactggtgc tgctcaaatg gacggagcta
    45541 tcttggtagt ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac atcctattgt
    45601 ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa caaagtagac atggttgatg
    45661 acgaagaatt actagaatta gttgaaatgg aagttcgtga cctattaaca gaatacgaat
    45721 tccctggtga cgatgttcct gtagttgctg gatcagcttt gaaagctcta gaaggcgacg
    45781 cttcatacga agaaaaaatt cttgaattaa tggctgcagt tgacgaatac atcccaactc
    45841 cagaacgtga caacgacaaa ccattcatga tgccagttga agacgtgttc tcaattactg
    45901 gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt ggtgacgaag
    45961 ttgaagttgt tggtattgct gaagaaactt caaaaacaac agttactggt gttgaaatgt
    46021 tccgtaaatt gttagactac gctgaagctg gagacaacat tggtgcttta ctacgtggtg
    46081 ttgcacgtga agacatccaa cgtggacaag ttttagctaa accaggtaca atcacacctc
    46141 atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga cgtcatactc
    46201 cattcttcac taactaccgt ccacaattct acttccgtac aactgacgta acaggtgttg
    46261 ttgaattacc agaaggaact gaaatggtta tgcctggcga caacgtaaca atggaagttg
    46321 aattaatcca cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt gaaggcggac
    46381 gtacagttgg tgccggtgtc gttacagaaa tcaaagctta attcacttaa gttttaaagt
    46441 aaagcaacat ttattcggac gtaatcgctc ggaacgaagg tcttaggatc tttgttccga
    46501 gcttttttat ttattgcagg atcaggaaaa aagacaggag tattataaat agaagaaaca
    46561 cgaaataaaa gagtcagtat tttctaaatg aaactttatg aaaacaaaat gttttttaca
    46621 agaaaaagcc ttgctttgtg agtttatata tattataatg acaaaggtgc gtttacttta
    46681 gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca
    46741 cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct atgtctactt
    46801 ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa
    46861 gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga
    46921 actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct ttacacaatc
    46981 atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg tacacacaaa
    47041 cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat gaagctagac
    47101 ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt
    47161 gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat cttcactgaa
    47221 tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt actacaagtg
    47281 aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga tatgcgtgaa
    47341 gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc
    47401 ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa agaaattaca
    47461 gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa aggtaaagga
    47521 ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca cggttctcgt
    47581 taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt taaaaacaaa
    47641 cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga agttgtacgt
    47701 gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc taaaaaatct
    47761 ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag gaggaactaa
    47821 ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga aatcacactt
    47881 aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg
    47941 caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc tgttcgcggc
    48001 ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc
    48061 tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc ttacagctac
    48121 aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga aaaagttgct
    48181 gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac aaaagaattc
    48241 aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct agaaaaaggt
    48301 aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt aacttctgat
    48361 aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca aactgctctt
    48421 actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa cgcccagtga
    48481 tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa gtggacactc
    48541 gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa
    48601 acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata
    48661 caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa atccaattat
    48721 tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga aaaacgtggc
    48781 gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc
    48841 tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa accatgctgg
    48901 acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca aacgccaata
    48961 tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc aaacgatcga
    49021 atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg gagtgaaagc
    49081 ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag gtccagaagc
    49141 agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta ctgttatcca
    49201 caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc
    49261 tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg gtgaagttcg
    49321 tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac aacacgaatt
    49381 aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg
    49441 tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag ctcctatcgg
    49501 acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa ctcgtaacaa
    49561 aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat tactttgacc
    49621 atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga aaggaccttt
    49681 tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa agaaaaaagt
    49741 tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat ataccatcgc
    49801 agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg taggacacaa
    49861 attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg ataagaaaac
    49921 gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca tcagctaaag
    49981 caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt gaccttatca
    50041 gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg
    50101 gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac tttgacttag
    50161 atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca atgaaacgtt
    50221 tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt catatcacgg
    50281 tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca tccaattgga
    50341 atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa agagtatgct
    50401 gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact tgctgatgct
    50461 gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc aattcacaca
    50521 gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa
    50581 ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa aaaaccagat
    50641 ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc
    50701 cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca agggatcaaa
    50761 actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg atactcagaa
    50821 ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga agaagcagat
    50881 acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat tcttccaact
    50941 aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt gtaaaacacc
    51001 gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg
    51061 gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa atcgaagctg
    51121 ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt aaaattttcc
    51181 ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt aaaggggcac
    51241 ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc
    51301 ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca atgaaaacta
    51361 agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga aatcagagaa
    51421 ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt gtttaatctt
    51481 agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga agtacgtaaa
    51541 tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc
    51601 attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca
    51661 gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca ccctatctat
    51721 ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa
    51781 gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa acgtttccgt
    51841 ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt cctatataga
    51901 ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa
    51961 gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg tggatctggt
    52021 cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt
    52081 ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct
    52141 cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat tattcgtgat
    52201 gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact acgtgaaaac
    52261 aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga cacttatcaa
    52321 aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt tatcactgga
    52381 aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt
    52441 gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt
    52501 ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt tgactctact
    52561 ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa
    52621 aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta ctgaatgaac
    52681 cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa atttaactat
    52741 agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg tgttggtgat
    52801 gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt gatcactggt
    52861 caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt acgtgaagga
    52921 atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa
    52981 ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt
    53041 gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc agaagtagat
    53101 tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac agcaaactct
    53161 gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa ataattaaag
    53221 gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt
    53281 tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag
    53341 ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat tcccggcgtg
    53401 aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga tcaatggtca
    53461 tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg gttaaacatg
    53521 agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg
    53581 aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg atccgtgttt
    53641 tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac
    53701 caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac ggtttaggta
    53761 tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt gaaaaaaata
    53821 tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag gaggtgtctt
    53881 taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac agttactcaa
    53941 gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac tttttcagca
    54001 gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa
    54061 gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt tgtaggtgtc
    54121 agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc acaattacaa
    54181 ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac accaccagct
    54241 ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc gaacaaagaa
    54301 gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc ttataaaggt
    54361 aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa
    54421 tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc tgaagtttac
    54481 cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac gtatttactg
    54541 cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa aaccagataa
    54601 gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg gtactgctga
    54661 gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa ttattgatga
    54721 cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt caggtggaac
    54781 aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg
    54841 cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg tgcaagcttt
    54901 agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat accattcatg
    54961 gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat taaccgtgta
    55021 acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac
    55081 aaaaacggac acgtagggtt tggtacaggt aaagctcaag aagttcctga agcaatccgt
    55141 aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg ttcaacaatc
    55201 ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa
    55261 ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc tggggtagca
    55321 gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg cgcaacagtt
    55381 gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt
    55441 gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt actttaaaac
    55501 gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta ggtcttacaa
    55561 agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg atcaatactg
    55621 tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact agaacattaa
    55681 ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc acgccaagta
    55741 cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg acgcggacaa
    55801 aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg gggacaaaca
    55861 ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa agactatgca
    55921 gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac tcctgttgct
    55981 ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg
    56041 gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca
    56101 attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta aaagatgctt
    56161 ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg tttgtatttc
    56221 gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa aacctttctt
    56281 ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa aacttttcca
    56341 ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag cttcttcaaa
    56401 tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac
    56461 tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct gtcggtatca
    56521 ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac gtagcaactt
    56581 acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg ctaggagaac
    56641 agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca ggtatcattt
    56701 ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat attgatcctt
    56761 cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc ttgttgatcg
    56821 tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa tatacgaaac
    56881 gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca gctggagtta
    56941 ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta caagcattga
    57001 gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt aactataata
    57061 cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc ttctacgcct
    57121 ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga agttatatac
    57181 caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg atgcgtttaa
    57241 gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt gctcaaatgg
    57301 tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc gttatcggtg
    57361 tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa tatgttggat
    57421 tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac acatttatta
    57481 ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa aggaacacaa
    57541 gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga tatgttccgc
    57601 gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat ggataaaggt
    57661 gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc tgaaccagat
    57721 acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc aaaagctctg
    57781 gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat ccatgttggt
    57841 gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg tggagcaact
    57901 taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg cggtgggcat
    57961 gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct ggctatcaac
    58021 atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct aaacactatt
    58081 gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag
    58141 cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc aataaattat
    58201 gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt tgagaatttt
    58261 gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga
    58321 ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt
    58381 gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg ctactgtttc
    58441 aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa cggttgaatt
    58501 gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat tgtactccgt
    58561 aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat gtgcgaacat
    58621 tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa tccaaaacat
    58681 aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca ggagtagata
    58741 ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt ggtaacacta
    58801 ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa
    58861 cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt gaaggggatc
    58921 ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct taccgtggaa
    58981 tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc
    59041 gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt gaaggaggtt
    59101 aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag
    59161 caggtattgc acatatccat tctacattca ataacactat catcatgatc actgatactc
    59221 acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga agcaaaaaat
    59281 caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca atggaacacg
    59341 gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa gcagcaattc
    59401 gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact ccagttcctc
    59461 ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc attttgagtt
    59521 ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa gataagaatg
    59581 attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta tggcaagttc
    59641 atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct acgtcgtatt
    59701 ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg tgtactgcat
    59761 gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt gaacattaaa
    59821 ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga
    59881 cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat tcttaacaaa
    59941 gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt aacagtgaaa
    60001 cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc aattggcgtt
    60061 cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt tgagaacaca
    60121 cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac agatggttca
    60181 attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca tttggatatt
    60241 tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca
    60301 caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt tcgctcttac
    60361 aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa atctgagcct
    60421 gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa agcaaaattg
    60481 cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag gagggaaacc
    60541 aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat gttgcgtgat
    60601 ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa
    60661 gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt gcatgcacgt
    60721 cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac
    60781 atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa
    60841 cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga tggtgcacca
    60901 atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata gatgattctt
    60961 ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct gctcctcaag
    61021 cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc ttcaattacc
    61081 tgtcatgaca gatatgtttg aaggcataaa aaaacatttt aagcgagaaa taattgaaaa
    61141 ttttgtcttt ttttgatagt gttagtagga actttaggat gaagaaggag taatcatgga
    61201 gccaattatt gaattagaaa agataaatta taaataccag ccagatgatc ttcgccccgc
    61261 attgaaagat gtctccttta caattgataa aggtgagtgg atcgctatta ttggacacaa
    61321 tggttcagga aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg
    61381 gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat atttggacga tccgacagat
    61441 ggttggaatg atttttcaaa atccagacaa tcagtttgtt ggttcgacag tagaagatga
    61501 cgttgctttt ggtcttgaaa atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa
    61561 agatgcactt gagaaagtta gaatggctga gtttgcttca cgcgagcctg ctcgtttatc
    61621 aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt gccttacgac ctgatatcat
    61681 tattttagat gaagcaacca gtatgctaga tccagaaggt cgtgaagaag tgatctctac
    61741 gatcaagaag atcaaagaag aaagtcaatt gaccgttata tctatcactc atgatattga
    61801 tgaagcagct aatgccaatc ggatactagt gatgagacaa ggcgaacttg tccgtgaagg
    61861 tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc gacttaggtc tagatctacc
    61921 atttcctgaa aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata
    61981 tatgacagaa gaaaggatgg tggattggtt atggacatcc gttttgaaca agtagacttc
    62041 acttatcagc cgaatacacc atttgaacaa agagccttat ttgacatcaa tatgacgatc
    62101 aaagaaaaca gttatactgc attagtaggg cataccggaa gcgggaaatc aaccttactt
    62161 cagcatttga atgcgcttgt taagccaaca agcggtacag tccatattgg agaacgagat
    62221 attcagccgg atacggataa taagaattta aagcccatcc gaaaaaaagt tggcatcgtc
    62281 tttcagtttc cagaagcaca gctattcgaa gaaacggtag caaaagacat tgctttcggt
    62341 cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga aacattagaa
    62401 caagttgggc tggatgaaag ctatttggaa cgttcgccat ttgaactttc aggagggcaa
    62461 atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc cggaagtact tgtattggat
    62521 gaaccaacgg caggacttga tccccagggc agaaaagaaa tgatggagat gttttggcgt
    62581 cttcataagg aacagcagat cactattgta ttagttacgc atttaatgga tgatgtagcc
    62641 aattttgcag attatgtcta tgtgttggaa aagggacgaa tcgttaatag cggggagccg
    62701 caagaggtct ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt acctactgcc
    62761 accgagtttg cagaagaatt aatggctaaa gggatgaact ttgctacctt gcctttgaca
    62821 gctgaagaat tagcagacgc tatcgtccat tatgctggag gaaatgccca tgatgaataa
    62881 attgatattt ggtcgttata tgcctggaga ttccttgatc catcgattgg atccccgagc
    62941 taaattatta gccagttttt attttatcgg aatcattttt ttagcaaata attgggaaac
    63001 gtaccttttg ttggcatttt tcacattact ttctgtcttc ttgtcaaaag tagatttgaa
    63061 attttttatc cgaggcattc gcccattgat ctggctaatc ctcttcactg tagctttgca
    63121 ggtcttgttt acaagtggcg gagaaactta ttggagttgg ggtgttttcc atatcactga
    63181 atttggtgtg atgaacggcc tctttatttt ctgtcgattc gtgttgatca tttttatgtc
    63241 gactctactc acgcttacta caccgccttt ggaattatcg gatgcgattg agtacttgct
    63301 tcgtccattg aaagccgtac ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc
    63361 tcttcgtttt gtgcctacat tgatggacga aacagaaaag atcatgaacg ctcagcgcgc
    63421 tcgcggtgtt gattttggtg aaggaaacct agttcagaaa atgaaagcag tcattccttt
    63481 gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga
    63541 agcaagaggc tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc attggcataa
    63601 tagagacacg atggttatca ttgcttttgt attattaact gtgctactga tctttttaag
    63661 aggataaacg ttcagtgggc cgctatctat tcttttcttg ctgatctgtt tgcaagaaag
    63721 gatgcaagca gtgtatgaca agaaatatta gggtctgtgg cagactaagg tcacaggcct
    63781 ctttctttta tctgaaaaga aagaacgaaa aaattttata gaaaaatcat tattccagat
    63841 aaccttttag gaacaaagga gagaaaaaca tggtacgtta caaagcaatc attgcgtacg
    63901 acggaacaaa ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt caagaagaag
    63961 tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat cacggtcttt ggttcgggaa
    64021 ggacagatgc cggagtacat gcgatcgggc aagtaatcca ttttgattat ccagaggagc
    64081 gtccgttaga acgaatgcgt ttcgcgctag atacccagtc accagaggat attgctgtaa
    64141 gacaagtaga aattgtttca gaagacttcc acgcaagata tttggtcaaa gaaaagacgt
    64201 atcaatttcg agtggatata gggaaaccac gcagtccttt tcgacgccat tatgcaagtt
    64261 attttcctta cccacttgat ttggaaaaaa ttcagcgggc gcttcctgat ttattaggga
    64321 ctcacgattt cacctctttt tgtgcatcag gaagctcgat agaggataaa atacgtacga
    64381 tctacgaggc taagatggag gtaaatgaaa caaaagacga attgttgttt acatttaggg
    64441 gaaatggctt cttgtataag atgatacgga tcttagtagg aacgcttttg aaaattggca
    64501 atggccgatt gcctgaagat agtatcccag atattattgc gaaaaaagat cgtaatgcag
    64561 caggacctac agcacaccct gaaggacttt atttgtatga agtgaaatat gaataagagg
    64621 ttatgataga agtggtcagc ttcaagaaat aagaaggtat ttacgaaaat tacttctcaa
    64681 aattttgcaa aatttcggct tattttcgaa gaagctactt ctggaacacc gtttaagaaa
    64741 gaggtcgtga gcctgtcatt taactctgag tcacaaagag caatttcaaa aaacagcttc
    64801 tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg
    64861 ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttggtgcg
    64921 gacagtctga acaggttcca tcgtttcttt attttctacg tcttcttctg tgtgtgtttc
    64981 gtggttgttc ttctattgct ggatcaatcg tgccataaaa ggggcgattg cggtcaaggt
    65041 accagacata aagaataaac aatgcaatac ataggacaaa aaggattgca cctatcgtaa
    65101 atccttcagg caagtaactg aaagtgacag tatgtttacc tgctggaact tttatcatca
    65161 agaatgcgtc tttgaaattg ctgaccttga ctggtttccc atcaatttta gctttccatc
    65221 ctttgtcata aggaatagtt gtcaccaaca tggaatcttc agcggtattt acagtcccag
    65281 aagcttttct gccagtggct tccatttcga cacctttttc tttgatctga ttcattgcgg
    65341 cttgataggc tacaacatct agaccaacga ctttaggctc catgaaactt atttctttcg
    65401 tcccataaaa acttgcagta aatgtcactg ttgtctcttg aggataatag ccaagatcat
    65461 agtattgacc agaaatgttg atttgcgttt ttcgcgatgt accattgaca gtgatcgtcg
    65521 cactagaact ttctaattgc gcaaaattgg ttgggaacaa acttaagtaa gcttgtgtat
    65581 ttgcaggcac atggacttcc caagtgagct cttttggctg gttatctgct gtttctgagt
    65641 aagtgacgcc agtcgttgtc tttgtgatcg tcgtattcaa ctgattcttc aaagtaggtg
    65701 tatagaatgt aaaatactgt tcatttagac cagacaatgt attgatcaag tttgtctgac
    65761 tggtcaaatt atctgtcact ggctgctcaa catcattaac ggacaacggt gcagtaaatg
    65821 ctagtggcaa tgcattgaca ttctgataca gtgtatagtc gccgctttga ccagcttggc
    65881 ggaagccata tttactgaac gtgctttcgt ttttagaaat gttatatttg attcctgtaa
    65941 acccatccat cagcaaagta ttattgttat aacggatatt caaatttgtc cctcttgaac
    66001 ggaaacctaa tttatccagg taagaggaag aattacgatt tcttatagaa gaaaacaaac
    66061 tgataccact gtaaccatag ttgatcgaat cgtttgcaga tactggatcc agattttcca
    66121 agcggtaaaa cgagtcggaa tctttttttg tttcatttac aagagctttc aagtcaggat
    66181 aaggatcagt atataaactg cgggaagcat aattccaatc atcaagaatg ccatggatca
    66241 tactgttcgt attgaccagt gcttcagcag acatgatcaa taataacaat atactcagat
    66301 gattgaatga aagtttttta tgctgataaa atcccacagc aaaagcataa atccctaaga
    66361 aaaccactgt caatacaaag gaagtcatcg tcacataggt gtagcttcct tcaggtttga
    66421 tgccccatgc caccgcaaag agagcagcta cgaccaaaat gattcctaac aacagcccac
    66481 catcgtcttg ttctatcttt tcccatcctt ttcctgcaag caggagcact aagaaagaga
    66541 acagataggc ataacggaat aaaaacatat tcggtgcatg cataccatgc cagaacaaat
    66601 tcaatggaac aagataaaaa cttgcgataa gtatggcgaa aagactgccg tacaaaaatt
    66661 tatttttcaa cggtactttc cgactggcaa aataaaacag acagaggact aatggtagca
    66721 gacctgcgta aacgaacgga atcgatccgt attttgtcgt gtcataaaca ccaatcatat
    66781 ttttcatgat tagatccaga taagaagtcg cttctgtttt caacgtagtg atctgactca
    66841 gtgcctcgcc atttgaccga agatccagca ctgctggcaa tacaatgatc attgaagcgc
    66901 cgcctgccag taaagaagtg ataccataag gaacaatcgt ttttttatat tctgaccagt
    66961 ttgtcaatag acgggcgata aaatataaga aagaaaatac gccaatcata aaacccatat
    67021 aaaaactgga taaaaacaag agtaaataac tgataaataa aagtttcggc cgtttaagat
    67081 ccataacacg atgaatcccc aaaatcacta atggaagata aacaaatgca tccagccaca
    67141 tgatgatctc cgaatgtgca gtgataaatg agatcaatgc gtaactgacg ctaagtgcaa
    67201 catgccccca gcgaggaatc ttgaacgtac gtgcaagaaa ccaaaaagcc aatccggcac
    67261 agccgacctt aagcaaagtc agaacataca gtgcatctgg catcatctgg tttggaaaaa
    67321 agaacaccag tggcgtgaag atcccgccta gatagtaaga aattaaagat aaataattca
    67381 atcctagaga agcattccat gtataaaaaa tactttgctt tccaagaaga acatttcgga
    67441 agctggcatg aaaattagaa aactgtgaaa aggcatcact tgccaaaata cttctgctgc
    67501 ttccaggata gattccgata cttagataaa caagtgctaa aatcaaaaac ggaatcagga
    67561 aactagctgc tgcgtagcgt ccgttttctc taaagaatga ctttaaattt tttttcatat
    67621 ttattttcca ccaaatttct atgaagttgt attcagttta tcatatcctt gtttttctct
    67681 gcttaaaaag agtctagttt tcacaaaaaa ataaaattta gaataaataa cttcataatt
    67741 tgttcgtata acaaaaataa acgcaaaaaa agggtaggaa tcaaaagtcc cacccttaaa
    67801 aatttttatt aaataaaaaa ccgttcacta tctgaacggt ccaagtatca ttcaattaaa
    67861 cgcgttcaac ttttcctgat ttcaaagcac gagttgacac ccaaactttt ttaggttttc
    67921 cgtcgaccat aacgcgaact ttttgtaagt taggtttaac agtacgtttt gttgagttca
    67981 tagcgtgtga acggttgttt ccactttttg tcttgcgacc tgtaaagtaa caaacttttg
    68041 ccatggtaat ttcctccttt gccttgattt ggagcaatag aataattcag ctcataccag
    68101 actaacatat catatagaat gggaatttgc aagtacattt tcatagatac aattgacaat
    68161 tgagaagaat aattgtaaat taaacaaggt tatacctgaa attttgcaag gtttttgagt
    68221 actttgggta actgcctttt atttcaagtt gcgctatgat aaaatatgat agaagaaaaa
    68281 tgggagtttt ttacccgtta aggaggctca ttcatggctg taaaaattca aacgtcagct
    68341 ggaaccattg aaattaccaa tgatgtaatt gcaacagttg ttggtggcgc cgcgacagat
    68401 gtgttcggtg ttgtaggaat ggccagcaaa aatcaaatta aagataatat tactgagatt
    68461 ttgcgtaaag aaaattactc aagaggagta gttgtccgcc aagaagataa tggtattgct
    68521 gtagatgttt atacgattat cagctatggt acgaaaatct ctgaagtttc ccgcaacgtc
    68581 caagaaaaag tgaaatacaa tctagaggca ttattaggcg taactgccaa ttcagttaat
    68641 gtctttgtac aaggtgttcg tgtactgcca gactaggcag gagactggct ggtaactcag
    68701 cagtcgatct gaaggaggat tatctaggtg agtgtaacag aaataagtgc aagccagttc
    68761 caggaaatgg tccaggcagg tgcgaatcgt ctgcaagtaa atgcagaata cgtcaattca
    68821 ttaaatgtat tccctgtgcc agatggcgat acgggaacaa atatgaatct atctatgact
    68881 agtggagcta aggcagtagt agattcaacc tcagaaaaag taggagaatt agcagcaatg
    68941 ctttcaaaag gattgctgat gggtgcgcgc ggaaattccg gcgttatttt gtctcaattg
    69001 tttcgtggat tttctaagca aattccagat gttgtctcat taaatgcttc tgatcttgct
    69061 gcagccttca ctcatggagt tgaaactgcg tataaagcag taatgaaacc agtagaagga
    69121 acgattttga cagtagctag agaagcagcg aaagctggag aaaagaaagc aaaaagtact
    69181 gatgatgtcg ttgaagtaat gacagctgtt gtaaaaggag caaagcgtgc tttagctaaa
    69241 actccagact tgcttccagt cctaaaagaa gtcggtgtcg tagacagtgg agggcaagga
    69301 ctgctgtttg tttatgaggg ctttttaagt gcattgaatg gagaattcca agcggatgaa
    69361 acatatgaac cttctccagc agaaatggat gatatggtca atgcagaaca tcatcgcagt
    69421 gtccaaggtc aaatggcgac agaagatatc cattttggtt actgtacaga aatcatggta
    69481 aaaatcggag aaggacctac agtcgatagt acattcgatt acgaagaatt ccgtaactat
    69541 ttagatggta taggagattc tttacttgtg gtcaatgatg acgagatcat caaagtccac
    69601 gtgcatacag aaaacccagg agaagtcttg aattatggac aaaaattcgg ttcattagtc
    69661 aaaatcaaag tcgacaacat gcgcttgcag catgagacga ttttggaaca cgatgaagaa
    69721 gtgaacgagt ttgcgaaaaa agaaatacca cgtatcccgt atggtatcgt agcgatcgct
    69781 gctggtaaag gcgtccaaga gctgtttgaa agtttaggcg ctaattatgt tatcagtggt
    69841 ggacaaacaa tgaatccaag tacggaagac attgttaaag cgattcaaga agtaaacgca
    69901 gataaagtga ttgtcttgcc aaacaataag aacatcttca tggcagctga tcaagcagca
    69961 gaagtagcag atatcccagt agcagttgtt ccttcaagaa cgatttccca aggattgaca
    70021 gctatgcttt cattcaatga tcaagcttct ttagaagaaa acaaagaaac gatgacagaa
    70081 atgttgtcta gcgttgtgag cggtcaagtc actcacgcga tcagagatac ctcaattgac
    70141 ggtgtagaga tcaaagaagg agactttttg ggcatgatcg atggaaaaat cctgatctct
    70201 aacccagata tcacagcaac agcgttaagt acactagaac aaatgatcaa tgaggacaca
    70261 gagatcgtaa cgatcttggc aggtgatgaa ggaacagttg aacaagcaaa tgaatttgct
    70321 gagaaactaa cagaaaaaca tcctgatttg gaaatcgaga tccaccaagg agatcagcct
    70381 gtctatcctt atctattttc tgctgaataa tttctgtgtc agaatatctt tctaacacag
    70441 tattcattca taaaaggcct tcggttagtg atcaggtatg atcgctgacc aagtcctttt
    70501 tttgttaaag gagggaggaa aaatttatgc gttctttaga tgatccaatc actatgctgg
    70561 caggagtcgg agaaaaaaga gcagctagtc ttgccagctt gggcatccaa acgattgaag
    70621 acttactgac ttattatcct tttcgctacg aagatattca ggaaagaaat ctaaacgaga
    70681 tccaggatca agaaaaggtt actttgaaag gaatcgtagt ctcgcctcct gtgatgaatc
    70741 gatttggtta taaaaaaagc cgattgcaat ttcgtatgat gcaagaacat gatgtgttca
    70801 acgtttcttt ttttaatcaa ccttatctaa aagaaaaagt cattttatcg gaggaaatcg
    70861 ccgtatacgg taaatgggat gcaaagcgta aagccttgaa cgggatgaag attttaggtt
    70921 ctcaaacaat ggatgacttt tcgccgatat atcacgtcaa caaatcaatt cgtcaaacga
    70981 cattagttga tctaatcaaa aaagggtttc aagaatacgg cacattgatt gaagaaaatc
    71041 tgccaaacaa ccttgtggag aaatatcgac tgcttgaccg agcttctgct gtaagaagta
    71101 tgcattttcc aaaagaccac gaagaaaacc atcaagccaa acgacgtgtc gtttttgaag
    71161 agttcttttt atttcaaatg cgtcttcaag gattgaaaaa agcagaaaaa gcagaaacga
    71221 atggtttgga gatcctttat gatgtccaaa aattgaaaca atttactcaa aagctacctt
    71281 ttgaactgac aaatgcacaa aaacgtgtaa cgaatgaaat ctgtcgggat cttcgcagtc
    71341 cgcagcatat gcagcgactt ttgcagggag atgtaggtag cggaaaaaca gtggttgccg
    71401 cgattgcgtt atacgctacc gttacagcag gtttccaagg tgcgttgatg gtgcctacag
    71461 aaatcttggc acaacaacat atggaaagct tgcaacagct gtttgatccc aatgaagtaa
    71521 ccatcgcatt gattacgggg tcaacgaaag caaaagaacg acgtgagctc cttgaaaaac
    71581 tagagcaagg ggaaattaac gtcgtgatcg gcacacatgc attgatccaa gatggagtcg
    71641 aatttcagaa tttaggtcta gttattacgg atgagcagca ccgttttggc gtgaaccagc
    71701 ggaaagtact tcgtgaaaaa ggctggcgac cagatgtctt atttatgaca gcgactccga
    71761 ttcctagaac attagctatt acagcatatg gtgagatgga tgtatctatc attgatgaac
    71821 ttccagctgg acgtatcccg atcgaaacac gctgggtaag aacacctcaa ttggacagtg
    71881 tactggattg gacgaaaaaa gaactggcaa gaggacatca aatgtatgtc atctgtccgc
    71941 tgattgaaga atcagaagca ttagatgtaa aaaatgcagt ggaaatctat gaaaaacttc
    72001 gtgaactatt tgcaccaaaa tatgaagttg gcttgttaca tggtaaaatg aagaatcagg
    72061 aaaaagaagt aatcatggaa gccttcaaag acaaccagat gcagatatta gtttcgacta
    72121 ctgtaattga agtaggggta aacgtgccga acgcgaccgt catgctgatc atggatgcgg
    72181 atcgatttgg attggcacag ctgcaccaac ttcgtggacg tgttggacgc ggaagtgatg
    72241 cttcctactg tatcttagtt gctaatccta aaaatgaact aggtgtggaa cggatgaaaa
    72301 tcatgacaga gaccaccaat ggttttgtac taagcgaaaa agatctggaa ttacgtggtc
    72361 ctggtgaagt atttggcttc cgtcaatctg gtctgcctca atttgcgatt gctgacttgg
    72421 tgacggatgg aaatgttttg gaggtagcca gagaagaggc tgcagccctt tggcaaataa
    72481 aggattggca gatgcttcct gagtatcagc ctttagcgca aagcctccaa atagaaaatc
    72541 aggataatcg ttatttcgat tagcagactg tgctatactt tatactgatg acggaaaaag
    72601 gagtggaaac aatgaaaatt gccgtagatg caatgggcgg agatcacgca cctcaagcaa
    72661 ttgtagaagg cgtggcttta gcgcagcaag attttccaga agtagaattc ttactttacg
    72721 gaaaagaagc agaaatcaag aaatacttaa caaatgaaaa aaacatcacg attatccata
    72781 cagacgaaaa aatcaatagt gatgatgagc cagtaaaagc aattcgacga aaaaagactg
    72841 catcaatggt ccttgctgca caagcagtga aaaacggtga agcagatgct gtattttcag
    72901 cagggaatac aggcgcgcta ctagcagcag ggttgttcat cgtaggacgt atcaaaaata
    72961 tcgaacgccc tggcttgatg tctaccttgc cagtagtagg gaaagaaggt gccggtttcg
    73021 atatgctgga tcttggcgct aacgcagaaa acaaaccaga acatttgttg caatacggta
    73081 ttttaggttc tttttatgca cgtaaagtcc gaggtatcgg acgtcctcgt gtcgcactct
    73141 taaacaacgg aacagaagca acaaaaggta gtgaagtgac gaaaaaagcc tatgaactcc
    73201 ttcagaatga aacaagcttg aattttattg gaaacgtaga agctcgtgag ctactcaatg
    73261 gtgtggctga tgtagtggtc acagatggat ttacaggcaa cgctgttttg aaatctattg
    73321 aaggaacagc aatgaatatg atgaatctat taaaatctgc tattctaaat gaagggatca
    73381 aaggaaaaat gggcgctatg cttttaaaag acggcttacg ttctttgaaa gcagaaatgg
    73441 attattccaa acatggcggt gccgttttgt ttggcttgaa agcaccagtc attaaaactc
    73501 atggagcaac aggccccgat gctgttcgct atacgatccg ccaaatccac acaatgcttg
    73561 agacagatgt agttggacaa ttagtcgaac aatttgaaaa aagcgaagaa tagtatttgg
    73621 atatctgaag cgatccgttc cattgaaaaa aaggtggaca ttcctgaaaa atgccagtaa
    73681 aattagccat ggtgtaggta attgacaagt gtatgattct tggaggtgaa aaagttgact
    73741 cgcgaagaag tatttaacaa agtagcgaaa attatttcaa atcattttga gatagatacc
    73801 gataaagtga cggatgaatt gaacatcaaa gatgatttaa aagctgattc aattagtatt
    73861 atggaattcg ttttagagct tgaagatgaa ttcggtacgg aaatttcaga tgaagacgct
    73921 gaacaaattg aaacagtcgg tggcgcagta gattatatca gcagccatct taaataattt
    73981 caaataaaag caaagaagga taaaaagctg catgcagctt tttatccttc tttttttaat
    74041 ctttttgaca cgtaaaagtc atttttctga taattctggc gataaacaga aattatgttg
    74101 caaagtaaag cggttttcta tataattatt aataattcat tacacaaaga taagaataac
    74161 ctttgttata aaacgattta ttctgtgtaa tgtccttttt tattaagaaa aacttccatt
    74221 taggaagcag agaggggtat tcgattgtcc gataaccaat tattagaagt tcagaaccta
    74281 catacaggat tccgactcaa agatgactat tacgatgcag tagatgacgt ttcatttact
    74341 ttagacaaaa acgagatttt agcaatcgtt ggggaatctg ggtgcgggaa aagtaccttg
    74401 gctactacga ttatgggatt acttgatcct aataacacaa aaattacagg tgagatttta
    74461 tataacgatc tgaatttaat cgacctgaac gaaacgctct ataataaaat ccgcggaaat
    74521 gatatcggaa tgattttcca agatccgttg tctgcattaa atcctctcat gagaatcgaa
    74581 gaccagatca aagaaggact ttcctatcac acaaagatga atgcagaaca gcgtcaagct
    74641 cgtgcactag aattgctaga acaagtaggg attcctaatc ctgcgagagt tggacgccaa
    74701 tatccccatg aactatcagg cggtatgaga caacgtgtca tcatcgcaat cgcaatcgcc
    74761 tgcaaacctc caatcgtcat tgcagatgaa ccgacgacag ccctagatgt aaccatccaa
    74821 gcacagattc ttgatttact gaaagacctt caggaagaaa caaaaacagg gatcatcttg
    74881 atcactcatg acttaggagt cgtagcagaa atggctgatc gagtggcagt gatgtatggc
    74941 ggacagttcg ttgaagtagc aggtgtaaaa gaactgtttg atcatcctaa gcatccttat
    75001 acacgctctt tactgaactc tataccacaa gaaggaaatc atgatgcaga actccatgtg
    75061 atcgaaggtg tcgttccttc tctgaaaaac atgcctcgta aaggttgtcg gtttgcacca
    75121 cgtattccat ggataccagc aagcgatcat gaagaagagc ctatccttca tgaagtcgca
    75181 ccgaaccact tggtacgttg ctcctgctat aagcatttcc actttagaga cgaaaaagga
    75241 gaggtgtaga tgacagaatt aatcacaatc aaagacctga aagttcatta ccctattcgc
    75301 agtggattct tcaatcgagt aacggatcat gtatatgctg tagatggtgt ggattttatt
    75361 atcgaaaaag gaaagacata tggtttagtg ggagagtctg gctctggaaa atctactacg
    75421 ggaaaagccg ttgtgggatt agaaaaggta acagccggac aaatcatgta tgaaggaaaa
    75481 gatgtaacga agcgaagcaa ccgcaaaaaa atgggttata acaaggatgt gcagatgatc
    75541 ttccaagatt caatgtccag tttgaatcct aaaaagcgag tgttggatat cattgctgaa
    75601 cccatccgaa attttgaacg gttaagcgat caagaagaaa agaaaaaggt aaaaggactg
    75661 ctagatatcg ttgggatgcc ggaagatgcg ctatataaat atcctcatga attttcaggc
    75721 ggacaacgtc aacgtctagg agtagcccgt gctgttgcta ccaatccaaa actgatcgta
    75781 gccgatgaac ctgtatctgc tctggatcta tctgttcaag cgcaagtatt gaatttcatg
    75841 aaacgaatcc agcaagaata cggcctcagc tatttattca tttcacatga tttaggtgta
    75901 gtaaaacaca tgtgcgacaa tatcgcaatt atgtacaaag gtcgatttgt agaaatcggt
    75961 acaagagaag acatttataa tgacccgcga catatctata cgaaacgtct gctttcagct
    76021 attccgcaaa tcgatgtaga aaatagggaa agccacaaag aaaatcgacg aagagtagaa
    76081 caagagtata tccaaaacca aaaagaatac tatgatgcga ctggtcgagt ctacgatttg
    76141 cgtacgatca caactacaca caaagtggcg ttgaaagatg gaggtgcaag ctaatgtgga
    76201 aaacgattct gcgacgtgtt ttattgatga tcccgcaagt tattatttta agtgttctga
    76261 tcttcttatt agcccaagcg atgccggggg atccatttac cggtttgatc aatccaaatc
    76321 aggatccggc agtgatcgaa cagatgcgac aagcagctgg cttgaatgac ccatggtatg
    76381 tccagtactt ccgttggatc ggaaatgctt tgcaaggcga ttttggtcaa agtttcttat
    76441 acaaacgctc tgttgctgac ttgatcggcg aacggatcat caacacttta tatctatcga
    76501 tcttgacagt tgtccttact tatttgattg ctgtgcctct ggggatgctt gctggccggt
    76561 atcaaaattc aatcttggat aagattgtcg taatctacaa cttctttagt tttgctgttc
    76621 ctttatttat cttcggcttg atcatgctat ttgtttttgg ttatcgttta ggctggttcc
    76681 caacaagtgg atcacaaaca ctaggtttgc aaggtggatt tatcactcaa tggatagacc
    76741 gtttgaaata tattctgtta ccatcaatta cccaagcatt tttggcgact gccgtcacaa
    76801 tccaatatct gcgaagtgaa gtaatcgatt cgaaatcgct cgattttgtc agaacagccc
    76861 gttccaaagg ggtaccgacg aataaagtat tcagtcgaca tattttccga aatgcagcgt
    76921 tgccgatggc ttctcaaatg gggtatgaaa tcacttcgtt gattgctggt tctgtagtta
    76981 tcgaaaagat ttttggttat ccgggagtcg gaaaactatt tattgattcc atcggccaac
    77041 gggactatac cgtgatcaca tccttagtat tgattttagg aatcgcgacg ctagtgggga
    77101 cattactttc agatatcatc atgagtatcg ttgacccgcg tattcggatt caataaaacc
    77161 aatcagaaag gagaatggat cgatgagtga aaaaaacaat acagcagttc aagaaagtat
    77221 tcctcctatg gggatacgaa tgattgctcg agaatttaaa aaagataaaa ttgctatttt
    77281 ttcattaggt ttgctagtgg ttttattatt agtgatcttt atcggtgcga tgctgaccga
    77341 tcaagacaaa gtgatgtatg ttagtatctt cgacaaatac gctgcaccag gaacagtttc
    77401 gacacaagga caaaagttca ttctgggagc agatgaaggc ggacgtgatg tattaggaca
    77461 attgatcata ggtgctagaa actctgttac gatcggattt gcgatcacga tccttacttc
    77521 gatcatcggg gttggattag ggatcatctc tggctactac ggagggatca ttgacaacat
    77581 cttgatgcgg atcgttgatt ttatcatgat tctaccgatc atgctgatca ttatcgtctt
    77641 cgtatcgatt atttccagat acaatgtctg gtcctttata ttcatcatga gtgcatttta
    77701 ttgggtagct aaagcccgat tattccgaag caagacatta tctgaagcaa gaagagatta
    77761 tgtcagtgcc tcaaaaacaa tgggaaccaa tgattttaaa atcatgttcc gcgaaatcat
    77821 gccgaatcta agttcattaa tcattactaa tttgacgatg aactttgcgg caaacatcgg
    77881 tatcgagaca actttgactt tcttaggttt cggcttgccg gcaaatgtac ctagtttagg
    77941 tacattgatc ggttatgcaa gtaacggaga agtcttagtg aatcggcaat ggatctggtt
    78001 acccgcatca atccttattt tagttttgat gttgagtatc aattatgttg gacaagcatt
    78061 caagcgttct gcagatgcgc gccaacggtt aggctagtat agaagcgaag ggggaaatag
    78121 gaatgaagaa aacgattttt ggatttgcag cgctgatgtc tgctgtcgtt cttgcagcat
    78181 gcgggggcgg aggtacagat acctctggaa acaactctgg aggcaacggc aataacaaag
    78241 aggaattaac ttttccaatt gcaacaaaaa ataatgatga tgcaatcgaa gacggcgaat
    78301 tagaagctgc ggtcgcaacg gacagtcagt ttaaaggatt gttccagtgg gagttttacc
    78361 aagattcata tgatgcagct tttatggctc cttctcatga accgctattt gcaaacgatg
    78421 acgatttcag aatcacagac gacggagctg caacgcttga tctagatgaa gatacgaaaa
    78481 aagcgacaat caaagtaaaa gacaacgtca aatggtcaga tggtgaagat gtaacggcgg
    78541 acgatgtgat tttcccttac gaaatcattg ggaataaaga ttacactggt atccgttatg
    78601 acgacacttt ccgtaacatc gtaggtatgg aagaatatca tgatggaagt gccgacacaa
    78661 tctccggaat caaaaaaata gacgacaaaa cagttgaaat cgaatataaa gaagtaaacc
    78721 caagcatgct tcaatcgggt ggtggtatct ggtcatatgc agcaccaaaa catacgttag
    78781 aaggtgtagc aatcaaagat atggaatcaa gcgatcaagt acgtaaaaat ccagtgactt
    78841 ttggccctta ttacatgagt aatatcgttg caggagaatc agtagaatac ttgccaaacg
    78901 agcactactg gaatggtacg cctaaattga aaaagatcac gatgaaatct gtaccgatgg
    78961 caagtgcaac tgaagcattg aactctaaat tatatgatat gattttccaa atgccaacag
    79021 atacttacga tacctataaa gaaattgctg gctatacaaa cttaggccgt gaacaaactt
    79081 cttatactta tctaggattc aagttaggta aatggaatgc agatacaaag agtgtggaat
    79141 acaatccaga ttcaaaaatg gctgataaat cattacgtca agcaatgggc tatgcattag
    79201 ataataatgc agtaggtgaa cgtttctatg caggtttgag aagcaatgcg acttcattga
    79261 ttcctccagt attccagtca taccatgatg atgagatgaa aggattcaca caagatctag
    79321 acaaagcgaa aaaactatta gatgatgctg gctacaaaga tgtggatgac gatggtttaa
    79381 gagaagacaa agatggaaat aaactaacaa tcaactttgc ttcgatgtcc ggtggggaaa
    79441 cagcgcaacc attggcagat tactatatcc aacaatggaa agaaattggt ttggatgtcc
    79501 aatacacaac aggtcgtctg atcgaattca attctttcta tgataaatta gaaaacgatg
    79561 atcctgaaat tgatgtttac caagctgctt ggaatacagg aactgatcca aatccaagtg
    79621 gactatgggg accaaatgct gcgttcaact acactcgtta tgaatcagaa gaaaatacaa
    79681 aattgatcaa cgatatcagt tcaagcaaag catttgatga agagtaccaa aaagaagcat
    79741 atcaaaaatg gcaacaatat gcatttgaag aagcatttgc gattccaacg ttgttccgta
    79801 atgaagtatt gccagtcaac gaccgtgtca aagactggga ttggtcttat gatgtaccaa
    79861 acccatgggc aaatgtttct gttacagcag attcacgttc ataatcacta aaaatgaccg
    79921 aaaaagggcg atgtcctttt tcggtttttt gatatttttc tgtttatttc tctaatattc
    79981 aggtcaaggc gctgatttca tgacttctcc aataaacggt gttcaaagtc cagtttctcg
    80041 gtattaagtc aaatttatgc aaaaatatgg aaagccattt tcacaaaatt ttttcttcat
    80101 actcgaaact aaacgaattt ttcacaactt ctcgtcaatc actaggatta ttaaagcttt
    80161 acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat aaggaagtat
    80221 cgaaaaaata tccataaaag gaatgtggaa aatggacaac cagctgacaa aagaactaaa
    80281 agaaaactat aacatcgttt ttcacgatct aaatttgctt gaacaagctt ttacccattc
    80341 atcctatgtg aatgagcatc gcaatctgca attatcggat aatgagcgac ttgaattttt
    80401 aggagatgcc gttttagaac tgatggtttc tcagtattta ttccgtttat atcctcattt
    80461 gccagaagga aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca gcttgtcgaa
    80521 atttgccaaa gaatgtcact ttgatcaata tgttttgttg ggcaagggag aagagaattc
    80581 aggaggacgt acgcgaccag cattgctttg cgacctattc gaagcttttt taggcgcgtt
    80641 atatcttgac cagggattcg acacgaccca tgcattcatc gaaaaagtga tttttcctaa
    80701 agtcaaagca ggtgcttttt cacatgagat ggatcataaa acaaaactac aagaagtctt
    80761 gcaaaaatca ggtgatgtat caatcgaata ccgattgatc aatgaagaag gtccagctca
    80821 tgaacgagta ttttggatcg aagtttatgt agatgatcaa ttgattggca ctggacaagg
    80881 gaaatcgaaa aaattagcag aacaagctgc ggcggaaaat gcattggcag cactgtaaaa
    80941 gtaagtgagg gaaagctttg tatttgaaac gaattgaaat agccggtttt aaatcatttg
    81001 cagacaagac agtcattgat tttgaaaata gcgtcacagc agtggttgga ccaaatggca
    81061 gcggaaaaag caatattaca gaggctatcc gctgggtatt aggagaacaa tctgctaaaa
    81121 gtctgcgagg cggaaaaatg ccggatatca tctttgccgg ttccgatacc cgtaagcagc
    81181 tgaatattgc agaagtcaca gtgattttag acaatagtga tcattatctg ccattggagt
    81241 acaatgagat cagtgtaacc cgccgttatc gacggactgg ggaaagcgag tttttcatca
    81301 ataaacaaag ctgcagatta aaagatatcc aagaattgtt cttagattct ggattaggaa
    81361 aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc gattttcagt agtaaaccgg
    81421 aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt gaaatacaag cagcggaaaa
    81481 agaaagcaga acagaagctt tttgaaacag aagacaacct cagccgcgtc caagacatca
    81541 tccacgaatt ggaagaacag ttaactcctt tggccgccca gagtgaggca gcaaaggagt
    81601 ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt gctgaaatca
    81661 agacagcaaa aaaagattgg gacaacaaac aagcacagct tgcgaaattc aatctagaat
    81721 taggcaaact atctgagtcg atccaagaac aagaatccat tttggcgaag cagcgtaaag
    81781 aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca agtattatta gatctttcgg
    81841 aaaaactgaa gcagacagaa ggacaaaaag atgttttgca ggagcggaca aagcatacac
    81901 aaaaaagctc gcaagaatat caaacttcat tagcagaagc acaaaaaaag gttaaacatt
    81961 ttgaaaagct tcaagaaagc ttgatgaaag cagcagcaga aaaagaaaca gagatccaaa
    82021 aagctgaagc aaatctcata aaaacacagc aagaattaga aaaataccaa aaatcaacga
    82081 aagaattatt ggcagaatta agagatcaat atgttgattt gatgcaagaa caagcagctg
    82141 taggaaacga actgaagtat ctagaacgtc aatatataca agaaacagca aaaagcaagc
    82201 agacgttagc caagcaatcg gaagtagaag catcagtaga ccgattgatc ttacaaaaag
    82261 aagaattgac acaaaagcag gcgcagctaa aatcatcttt aactgaaaca aaagagaagt
    82321 tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa gaacagccta
    82381 aaatgtatca attaatgaat caagtccagc agctacgtgc tcgtaagaaa agccttcaag
    82441 aaatccaaga aaactatttt ggtttttacc aaggggttcg attggtcttg cagcataaac
    82501 agcagctttc gggtattgtc ggagcagtgg ctgaactgat cgatgttcct gcagatttca
    82561 cgttagctat cgaaacagct ttaggtgggg cagcccagca tgtcatcgtt gaaaatgaaa
    82621 aagatgctcg gcaagccatc acgtatttga aacagcaaag aggaggaaga gcaacattcc
    82681 ttccactgac gacaatcaaa ccaagacaat taccggctca tatattgaca caagcagcag
    82741 ctgtagaagg gtttataggg attgccagtg aacaagtcag ttatcccgat caaatccaaa
    82801 ctgtggttca aaacctgcta ggaacgatcc ttttagccaa agacttaact agtgccaatg
    82861 caatcgcgca aacgatccgc tatcaatatc gagtggtttc cttagaaggt gatgtgatga
    82921 atgccggagg atccatgaca ggtggagcga ataaacgagg aaaccaaggc agtttgtttg
    82981 tacaaaatca agaattgaaa cagttgacta gtgaatttga agaagcagat aaacaactcc
    83041 aagcacaaga aaaaaaagtt caggagctgc aacaagaaac agctcgttta gccgaagaac
    83101 aagaagtttt gcgtacacga ggcgaacaat tacgttttga agaacaagaa gcgacaaatc
    83161 agctacaaaa catcataaat gaacttgaac gttttgaaaa agaaaagcaa atctcaactt
    83221 ttgagacacg tgaactgcag cagtttatcg aagattacga aaagcaaacg aacgaattga
    83281 aagacaaaca aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa atcaaatcgt
    83341 taagtcaaga aagcgaccag atggaagcca gaagagcaca agtacaaagt caaaaagcac
    83401 aagaacaagc agatcttgct gtcttgaaag agcagtttaa tcatctgcag atccagctcc
    83461 gcggtgcccg agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata gaaaagcagt
    83521 tggcgacatt gacagcagac ttttctgatc atgaagtcac agaagaaagt ttggaaaaac
    83581 aaattaacga attatctgct cagcgagaaa cattaaaagc cgagttggca aaagccaaag
    83641 agcagcgaga tagaaggcaa aaggagatcg ataagcttga agcagtcttg gcagagcgta
    83701 atcgtgagca aaaagcacga ctgtcggaac aatccaaatt agaagtccaa aaagaccgag
    83761 ctgaaatgat tttagataat catttgacct atctgcagtc agaatatcag ctgacatttg
    83821 agaaagcttc ccaagactat caagaaacaa ccgatatcga agacagtaga acaaaggttt
    83881 catccttaaa agaacagatt gaaaaactgg gaccagtaaa cttaaattca atcgaacaat
    83941 acgaacaagt cagtgaaaga catacattct tagcaacaca gcgagatgat ctgttagcag
    84001 cgaaaaacca attgtttgaa acgatggatg agatggatga tgaagtgcgg acaagattca
    84061 aagaagtatt tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat atgtttggcg
    84121 gcggtaaagc tgaattagta ctgacggaac caactgattt gctgaaaaca ggtattgaga
    84181 ttgaagtaca acctcctggc aaaaaactgc aaagtctaag tttgctttct ggaggagaac
    84241 gagcacttac agccatcgcg ttgctctttt ccattatccg tgtctgtcct gttccgtttt
    84301 gtatcttaga tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt tttggtcgtt
    84361 acttgagcga tttccaagat gacacacaat ttatcgtagt cacacaccgc aaaggaacga
    84421 tggtcgcagc agatgtactg tacggagtga cgatgcagga atccggtgta tcaaaaatcg
    84481 tttctgtacg gatggaagat atcaatgaag aggcgaaaat caacgaaacg aaagtgtagg
    84541 aacgaaaatg atcaaattag tagcaatcga tttggacggt acattattaa atgaacaaaa
    84601 gctgatttca gatgagaata aacaagcatt agcacaagca aaacagcaag gggtcaagat
    84661 cgttctatgt acaggacgcc ccttagctgc gatggcgcat tatctgcaag aattaggact
    84721 ggtagatgaa ggtgactaca gcatcacttt caacggcggt ttggtacaaa aaaatgatac
    84781 tggtgaaatc atggaaaaga aagtgatgga agtatcagat atccaacgct tatacaaact
    84841 ggctcagcaa ttggatctgc cactagatgt tttgtctgac aatgtggttt tacagctgcc
    84901 tagtgcccca aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc aattccaacc
    84961 agcggatata agcgatatta cagatgaatt tgtgcttaat aaggcagtaa tcgcttatga
    85021 ccaagaagaa ttagatccta aaatcaaaga aataccagca gaattccatg aaatgtatga
    85081 gatcatcaaa acaagaagta tgcttttgga atttatgcca aaaggtgtta caaaagctta
    85141 tggtatttct ttattggcaa aagatcttgg tttgaaacaa gaagaaatca tggcgatcgg
    85201 tgatgaggaa aatgatttac cgatgatcca atatgctgga ctaggcgtag cgatggagaa
    85261 tgctgtgcct ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg aagaaaatgg
    85321 cgtagccaaa gtgatccaaa aatttgtatt agagtagggg gagtcaaatg ggattttttg
    85381 ataaaatcaa gcgtgctttt actggcgaag agaaaacaga agaagttgaa gtacaggaga
    85441 agtacgacaa gggtttagaa aagacgcgta aaacatttgg tgaacggatg aatgagttgt
    85501 ttgccaattt tcgaacagtc gatgaagact tttttgaaga attagaagaa acacttatcg
    85561 gtgcagatgt aggttttgaa acagcgatac gaattaccga agcattacgt caagaagtga
    85621 aactcagaaa tgcgaaaaaa tcggcagaag tccaaaatgc gatcatcgag aaacttgtgg
    85681 atctctatga agcggaagga atcaatgaag taaatgaact aaatatccaa gaagacggac
    85741 taacagttat tttgtttgtc ggtgttaatg gtgtagggaa aacaacaagt attggtaaac
    85801 ttgcccatca gttcaaaaac gaaggtaaaa aagtattgat ggcagcagca gataccttcc
    85861 gcgctggagc aatcgaccag ttagttgtct ggggcgaacg cgctggtgtt gaagttgttc
    85921 gtggcaatgc aggaggcgat ccagcagctg tcgtattcga tgctttggaa agagcaaaga
    85981 cagagcaggc agatgtttta ctagttgata ctgcaggccg actgcaaaat aaagtgaact
    86041 tgatgaacga attagaaaaa ataaaacgag taatccagcg ggaattacca gaagcacccc
    86101 aagaaacact tcttgtatta gatgcaacca ctggtcaaaa cgcgatggtt caagcaaaac
    86161 aattcaaaga aacaacagat gtgaccggtc ttgttttgac aaaattagat ggtacagcaa
    86221 agggcggtat cgtacttgct atccgtaatg aattgcattt accagtgaaa ttggtcggtc
    86281 ttggtgaagg aatcgatgac ttggagccgt ttgatccaaa tgattttgtc gttggattgt
    86341 tcaaaggatt actaaaagaa gaataaccca tgtctaaaaa gcaggagctg tgacaaaagt
    86401 tgcagctcct tgtttgaatg attgttttta acatagctgt gtttttgatg ccttcatttt
    86461 tatacttatt tgtaagcggt tctcaaacaa tttcgttctt tttcttagac tttttgatta
    86521 taattagatt catatgaatt ggaggtcact taatgatgaa ttttaagcaa ttattattgc
    86581 atgtcaatgc gcgccctttt atcgaccaag ctcgatttgg tatagaacgg gaaggacagc
    86641 gggttgatct tgcaggaaat ctagcaaaaa ccgatcatcc agcaatcttt ggcgatcgat
    86701 cctatcaccc ctatatccaa acagatttta gcgaaacaca aacagagatg atcacccctg
    86761 ttaccgattc tattcccgaa ttatttcagt atctcgctgc tgtttatgat gtgactgctc
    86821 gttctatacc gaaagaagag atgatctggc cattaagtat gccacctgcc ttaccggaaa
    86881 aagacgaaga gatcattatt gcaaaattaa aaaatttcga agatgtcttg tatcgacgtt
    86941 atttagcaaa agaatacggg aagcgaaaac agatggtaag cggtattcat ttcaattttg
    87001 aatttggcga tgagttgcta agaacattgt tcagccatca ggaagaattt caagattttt
    87061 ccgaattcaa aacagaactt tatttgaaaa cagcgagaaa ctttatgcgc tatcgctgga
    87121 tgatcactta tttattcggt gcttcaccaa tgagtgagaa gaactacttt ttagatgaat
    87181 cacatccgca agaacctgtt cgcagtattc gcaacagtgc gctgggctat acgaatcatc
    87241 caaatgtgaa agtttcctat gcttctatga aacagtattt agcagatatc gagcgaatga
    87301 tcgaagaagg gaaactttcg gaggagaaag aattttatac accgcttcgt ttccgaggcg
    87361 gaaagaaagt cgcagattta gcaacaacag gtgttcgtta tattgaattg cggaatatcg
    87421 atttgaatcc ttatgcaaga ttgggtatca atccagagca agttcggttc ttacaattat
    87481 tcctgatgta catgttatgg acagaagaaa aagaagactg tgaccaatgg gtagcagaag
    87541 gtacgactcg aaataacaaa gtagcactag aacaaccatc agatcaaaca gaatttcatc
    87601 aagaaggtag agaaattctt gaaggaatga agcaaatgct ggttgaattg gattggttgg
    87661 attctcttta tttagtagag gaagcattga ctcaaatgga tcatcctgaa caaacgttag
    87721 cagcgaaact ctatcaagaa gcacagctat cgagtcagca agaagttgct gtggcattgg
    87781 gacatcaata ttataaagaa agccatgaac gtccctatca gttggccggt tttcgcgaaa
    87841 tggaactttc tactcagata tttatgttcg atgccatcca aaaaggtgtc caagtcaaag
    87901 tattagatga gtcggatcaa tttttgcgtc tgcaattcca agaccatgta gaatatgtga
    87961 aaaatgcaaa catgacaagt aaggacagct atatcgtccc acttatcatg gaaaacaaaa
    88021 cagtaacgaa aaaagtctta aaagaagctg ggttccgggt accaggaggc gcagaatttt
    88081 catccatgga agaagcggta aaagcttatc cgagatttgc cgagcaggca tttgtgatca
    88141 aacctaaatc aaccaattac gggttaggta tcacgatttt caaggaagga gctagtctgg
    88201 aagattatca agcaggttta gccattgctt ttcgtgaaga cagttcggtt ttagtagaag
    88261 aatttatgcc gggaacggaa tatcgtttct ttgtgatcga cggagaagtc caagccatca
    88321 tgttgcgagt ccctgctaat gttattggtg acagtatccg aacagtgaaa gaacttgttg
    88381 aagaaaaaaa tagtgaccct ttgcgtggaa cgaatcatcg tgcaccttta gaattgatcc
    88441 aattaggtga attagaacaa ttgatgttga aagaacaagg gttgacgatc gaatcagttc
    88501 cccaagccaa tcaaattgtt tatctaaggg agaactcaaa tatcagtaca ggtggtgatt
    88561 cgatcgatat gactgatgaa ttttctgaag cttacaaaaa aatcgccgtt tctgcggtag
    88621 aagcactagg agctaagatt agtggtatcg atttgattat accagataaa gaaatcgatc
    88681 ctacaactga taaaaaagca tatgggatca tcgaagcaaa tttcaatcca gccatgcata
    88741 tgcatgttta tccttttgca ggaaaaggca gaagattgac gatgaatgta ttgaaattgt
    88801 tatatcctga agtattttaa aagaaaaagc ttttgacaga ttgtcctatt tgatgtcaat
    88861 ctgatcaaaa gctttttttg catgtaaaaa tttatgtgtt ttttcgtttt aaaataaacg
    88921 atcccagaag ctttttttct tttttggttt ttctttttca gctggcgaag agttttcttc
    88981 tgggtatttt ttttcgatat caatcacagg ttcatttact gcttcttttg ctgttaagag
    89041 caatccgaat tcctcaggat ttgcagtgac gtaatcgttg actacagtga aacggctatt
    89101 gtttttcgtt gctagctgga tatattggtt ttggacagaa aggggtagag ccccattcaa
    89161 gagtatggaa gcatctggat gttttttgag ttcttttaaa aagttttctt tgttttttgg
    89221 ttggatcatt tcagcaacag tcatactaag atagcagcgt tccctgaatg tccctaaata
    89281 tttatgctgt tcttcagggt tcactaaagg tgtaccataa agtcccttat ccaaatggct
    89341 ttttacatca tcttgtgcca taaaaaatac cctccttctt ctctttgtct ttcttacgat
    89401 tatagcatat tcccaataat acctaaaaca tatgaaaaag cggttgcttt tgttcaacga
    89461 ttcacttaaa ataagaatga aaatcattct caattagaaa ggtggattgg atgcgctacg
    89521 gacgactagt agcattatta gttattttaa gtagtatttc attgttcatc ggagtatcga
    89581 atgtacaatt gtcagaactg ctccagttag atgggcaaag cttacaaatc ttgctagtga
    89641 ctcgtttgcc acgaacgatt tgtttgatcc ttgtgggagc gaccagtagt atttgtgggt
    89701 tgatcatgca acatttgacc caaaataaat ttgtttcacc aacgactgcg ggcacgatgg
    89761 atagtgcgcg tttaggtatt ttagtggcta tgattttcct acctggtgca tccttgattg
    89821 tccgatcctt gacagcattt tgctttgctt ttgcaggaac attgcttttt ttatccctta
    89881 cgcgattgtt cctccagaaa aatcaattga tgcttccgtt gattggagtc atgttaggga
    89941 atatcattgg ttcagttgct actttttttg cttatcaatt ccagttggta caaaatatga
    90001 cttcatggct tcaagggaat tttgcgactg tgatacgagg aaattatgag ttattgtatg
    90061 ctgttgttcc attgttgctc ttggtttggc tatttgctta ccgatttacg atcattggtc
    90121 tgggcgaatc gtttgcagta aatctgggaa tagattacca gaagatgcag tttttaggca
    90181 ttggcttagt atcattggct agtgcagtca tgttgatcat ggtaggaagc attcctttta
    90241 tcggagtgat cattccgaat cttgtctcta gaatatatgg agatcaagtg cacaaaacaa
    90301 ttggcgtaac tgctttagca ggtagtttgt tcttagtatt ttgcgatctt gtcgctcgcg
    90361 tgttgatttt tccttatgaa ataccagtca gtgtgatcgt tggaatcatc ggtggggcta
    90421 tcttccttta tcttctgata aggggacgac gatatgctta aaaaaaagcc atctattttt
    90481 attggtatgc ttgttcttgt tttatttgtt acaggaatgt acttgacgat ccatacatat
    90541 ggaaactggc attttgcatg tgcattgagg agtaagaaaa tttttgcatt tatccttgtc
    90601 tcgctagctg tcagcagtgc cacaatcagc tttcagacaa ttacgcataa ccagtttttg
    90661 actccttcag tattgggact tgagtcgtta tatgtccttg tccaaacggt ccttttcttc
    90721 tttatcggag gagtgacgat gcttcagcag acaggtacag tttttttctt attgaacgtt
    90781 gggctgatgg tcgggctgag tctattgtta tttttctttc ttttgaaaaa aatgagagcg
    90841 aatctttttt tattactgat gtcagggatg attttaggta cgctttttgg tagtgtcagt
    90901 acctttttac aagtcatcat ggatccgaat gaatatgatt tgcttcaagg cagattattt
    90961 gcaagctttg gcaatatcca gagtcagtat cttttgcctg cttctttgat cattatcggc
    91021 tgctggtacc tgttatttcg atatcgacat gtgctagacg ttttccatct cggaaatgaa
    91081 caagcagtaa acctcgggat agacacagat cgttttgaat ttttgatttt atgttgtgtc
    91141 agtcttttga cagggacatc aacggctctt gtgggtccag ttactttttt agggtttatt
    91201 gtcgcaaata tcagttatcg tgtcttttct acttatcgtc atggtcagct atttatcgga
    91261 agctttctgg tagctatgct gtttctgaca ggaggacagc tcgcagtaga gcagattttc
    91321 ggctggaata caacagtgag tgtcgtcgtt gaatttgtcg gaggaattta ttttgtcaca
    91381 aaattgattc gcgaaaggaa gagtatccta tgattgattt acaaaaggtc aataaactgt
    91441 accatggaaa atcagtggtt aaagatgtac agttaacgat tcctgaaaag aaattgactg
    91501 cttttatcgg tccaaatggt gctggaaagt caacggtgtt gtcgatgatc agtcgtttga
    91561 taccaaaaga tacaggtgaa atctatttag accataacga agtaaaggct tggaaatcca
    91621 atgaattagc caaaaaatta tcgatcctac gacaatcgaa tgaaataaat gtccaaatca
    91681 ccgtgaaaga attagtagaa tttggacgat ttccttatac gaaaggaaaa ttgacgacaa
    91741 aagacgagga aatcgttcag gaatcactgg aacaccttgg gttagaagaa atagcggatc
    91801 aatcgattga cactctttca ggcggtcaac tccaacgtgc gtatattgca atggtgctag
    91861 cgcaagacac ggattatatt ttactggatg aacctttgaa caatctagat atgaattttg
    91921 cggttcaaat catgcagatt ttaaaaaatt tagtacaaga aatcgggaaa acaattgtta
    91981 ttgttcttca tgatatcaac tttgctgcaa gttatgcaga cgaaattgtt gcaatgaaag
    92041 atggacagtt atttacgcaa ggaacgaccc aagaaatgat aaaaaaagat gtattggatg
    92101 aattatacga aatggacata cggatttgtg agattgaagg ccgcagattc tgtctttatt
    92161 tctaaaacta acattaggag agaagagatg aaaaaattag tagtaggtat cttgattcta
    92221 gcatcatttg gtttagcagc atgtggaaat agcaacaaca ccagtcaggc agacacaaaa
    92281 aaaagcagca cacaaactga aactacattg acgataacag atagtaatgg agaccaaatc
    92341 gaggtgccaa ataatccaga aaaagtcgtc gtattcgaca atggttcttt agatacgatg
    92401 gatgctcttg gtgttggaga taaggtagtt ggagcagcta caagcagcct tcctgagtat
    92461 ctttcatcct ataaaaaagt agaatcagca ggtggaataa aagaaccaga cttagaaaaa
    92521 attaatcagc ttcagccaga tctaatcatc atttctggcc gacaaagaga ttttcaaaaa
    92581 gatctaagtc aaattgctcc gaccattttc ctttctttag atgcgaaaaa tccatgggaa
    92641 tctttccagc aaaatgtcac tgctttaggg gagatctttg gtaagcaaga agaagctaaa
    92701 actcaattag aggaattatc tagtgcaatc gatcagacga aaaagaaagc agaagctact
    92761 gacaaaaaag cactagtcac acttgtgaat gaagggcaat tatcagccta cggatctggt
    92821 tctagattcg gtttcatcca tgatttattt ggctttgaac aagcagatga tcaaatcgaa
    92881 gcatccactc acggtcaaag tgtttcttat gaatatgtgc tggaaaaaaa tccggatatc
    92941 ttatttgtag tagacagaac aaaagctatt ggtggagacg acagcaaaga tgatatttct
    93001 gccaatgaac tggtagcaca aacgaatgct ggtaaaaatc agcagatcat ctcccttgaa
    93061 ccagatgtct ggtatttaag cgggggcggt ttagaatcaa tgaaactgat gatcgaagat
    93121 gtgaatcaag catttaaata gcataaataa cagaggatta aactctcata ctatgtaaca
    93181 ataatgtttc ttacattctt aaatagcaag agcctatcct caaagaagtt atcaaaaacc
    93241 atccgtaaaa tcgtattgat gattttacgg atggttttta tttatttgag aaatcttttt
    93301 tcaacgtgaa ttcattatct acatcatacg ggttgattct agaatgggga gtggcaaaaa
    93361 atagtgtgat ccattttttc agcatcaaga tcgcctcctt tgatttgaat caaaggtaca
    93421 aagcgaaact tagataaaac ttaaatttag atatttttat tttgtaccga tctcttcttt
    93481 aaacgatgtt ccatctgctg cttctcggca ataagtccac tagactagaa aatatgagaa
    93541 gctattttct gtccagcgtt cttattactc aaagcaaatc gcagttgtca caatctcttc
    93601 taaacgatgt tccatctgct gcttctcggc aataagccca ctagactaga aaatatgaga
    93661 agctattttc tgtccagcgt tcttattact caaagcaaat cgcagttgtc acaatctctt
    93721 ctaaacaatg ttcaatcagc tgaccttcgg tattagctca aagcactgat ttcacaacct
    93781 ctatttaggc atgtgtctca gcaagcggtt gatagttgat gatctgcatt tttcgataaa
    93841 aatcggcggg aagcttactc caccgtttta ataattgata gaaataactc tcttctgctt
    93901 tgattttata ctcgcttaat atcaatccaa aactattcgt tttttttgaa gctaattttt
    93961 tataaatatc caatgctttg gttttatctt cattgacaaa gagtgttttt acttcaatat
    94021 aagaaccctc gatattttgt gtttcttcga taaaatagat gttttttata tattccttca
    94081 tagtgattcc tccaaagaag tcttttattt gataagttta ctctaccgaa gagttgttaa
    94141 taatccgtga acaaaaagta attctttttc caatagttgt taaaggtttt ttatttttta
    94201 tagtattttt tgttatttaa gaaaggaata aggttaaaca aataacaggt tgctttttgg
    94261 gtctttttcc gttcaagaaa ctgaacgaag aaaagggaaa gttgtctctt ttgtgataag
    94321 agaaatcata ataaacgaat gacttacttt ccaataaatt gagaacagtc agaaccagac
    94381 aaattagttg catctgatga ataaagtgat tattctatga catgaggtga tacagatgaa
    94441 attagaagtg acaaaagaag cacaagaagt attgaagaat aaattagaag cagacgatca
    94501 acttttatta gatatcgaaa acggtgacgg tccatttgca gaaagccaag tcagttgcca
    94561 attggatacg gcatttcgat tgatcattgt caaaaaagat acacaaacag atctaagtct
    94621 ttattctgta gtggctgata caccagttgg accaatcaaa atcaaagaca gtgcaatact
    94681 ttacatggat gatccatcca cacttgcttt agaaccaact tataatagtt tacaattaaa
    94741 aggaccaagc ggcttgagaa aaggcaactt gcaagttgtt cgaaaagact agagggataa
    94801 aatgggaaaa ttggcgatca tcagcgacct acatgtggac atcaataaat tcggcgaatc
    94861 agagctgatg cagctttggc aagttttgca aaagcacaag atcactcggc ttcatctagc
    94921 cggagatacg gctaataaag tagaccgttg cctagctgtt gtcgaatttt tcgtcaataa
    94981 aggattatct acgacatttc atttcggcaa tcacgagttg gcagatgtgc ccaatgaaca
    95041 gatgatggac cattttccag acccacattt tttaaatgaa cggtatattg ctttaaatga
    95101 gcagaccgtt cttttaggga tgaatggctg gtacgactat cagttttccg aattgaaaga
    95161 ttacgaggca atcgtacgaa tgaagcgttt gtattggtat gatcggttca tccagcgtgg
    95221 gaaaacagat ctcgaggtca atgcagaaat gcttgcagct gcaaaaaatt tgctagacca
    95281 gctggaagca aaaaaacttg aggtgatttt atccactcat ttcgttccta aacgggcatt
    95341 tatcatgtat caatctgctc cttatgagcg ttggaacaaa ttaaatgcat ttttaggatc
    95401 agaatcattc ggagaattac ttgaccgtta ttcaaatatc aaacaagtag tttttggcca
    95461 tacgcatcgt cgctttgaag ataaatccat cggtcacacg atttacagct gcagaccttt
    95521 tggttattat tatgaatggc agctgacccg agattttgtc ttgaaagaac agttgctaga
    95581 cagctatgat cctatgaaat tacgtgtcct cttgcgtgcc catcacccag cttttcaggc
    95641 ctatcaaaca caacatttgc aagaggaatt tgaacaagcg atgacaatcg ttccgtattg
    95701 agacagcatt agtaatgtgt aaaagaaaat accttgtaaa acataaggat aagtaagagc
    95761 agtagatcca tttatcgtgg atatgctgct ttttttgcgg aaaataatca gcgaatgttt
    95821 atttgatcgg atgatttgta actcgcagag tactagatgc aagcgtgtat tctttttttg
    95881 aacgataatc acaaaaaaaa atata
//
LOCUS       DAFCNJ010000004        89653 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000004 DAFCNJ010000000
VERSION     DAFCNJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.004"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(553..2166)
                     /locus_tag="LNE35_000378"
     CDS             complement(553..2166)
                     /locus_tag="LNE35_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6909567.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="LNE35_000379"
     CDS             complement(2307..3215)
                     /locus_tag="LNE35_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6909568.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3398..4522
                     /locus_tag="LNE35_000380"
     CDS             3398..4522
                     /locus_tag="LNE35_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6909569.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4749..5666
                     /locus_tag="LNE35_000381"
     CDS             4749..5666
                     /locus_tag="LNE35_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6909570.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5659..6492
                     /locus_tag="LNE35_000382"
     CDS             5659..6492
                     /locus_tag="LNE35_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6909571.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6575..7693
                     /locus_tag="LNE35_000383"
     CDS             6575..7693
                     /locus_tag="LNE35_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK6909572.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7687..9585
                     /locus_tag="LNE35_000384"
     CDS             7687..9585
                     /locus_tag="LNE35_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBK6909573.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9579..10802
                     /locus_tag="LNE35_000385"
     CDS             9579..10802
                     /locus_tag="LNE35_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK6909574.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10888..12822)
                     /locus_tag="LNE35_000386"
     CDS             complement(10888..12822)
                     /locus_tag="LNE35_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6909575.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13048..13704
                     /locus_tag="LNE35_000387"
     CDS             13048..13704
                     /locus_tag="LNE35_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK6909576.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13778..14131)
                     /locus_tag="LNE35_000388"
     CDS             complement(13778..14131)
                     /locus_tag="LNE35_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK6909577.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14323..15252
                     /locus_tag="LNE35_000389"
     CDS             14323..15252
                     /locus_tag="LNE35_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBK6909578.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15263..16099
                     /locus_tag="LNE35_000390"
     CDS             15263..16099
                     /locus_tag="LNE35_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBK6909579.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16347..17123
                     /locus_tag="LNE35_000391"
     CDS             16347..17123
                     /locus_tag="LNE35_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6909580.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17299..18042
                     /locus_tag="LNE35_000392"
     CDS             17299..18042
                     /locus_tag="LNE35_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909581.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18035..19615
                     /locus_tag="LNE35_000393"
     CDS             18035..19615
                     /locus_tag="LNE35_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBK6909582.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19866..20345)
                     /locus_tag="LNE35_000394"
     CDS             complement(19866..20345)
                     /locus_tag="LNE35_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK6909583.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20361..21113)
                     /locus_tag="LNE35_000395"
     CDS             complement(20361..21113)
                     /locus_tag="LNE35_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK6909584.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21230..21300
                     /locus_tag="LNE35_000396"
     tRNA            21230..21300
                     /locus_tag="LNE35_000396"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21262..21264,aa:Gly,seq:ccc)
     gene            21661..22386
                     /locus_tag="LNE35_000397"
     CDS             21661..22386
                     /locus_tag="LNE35_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBK6909585.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22641..22907
                     /locus_tag="LNE35_000398"
     CDS             22641..22907
                     /locus_tag="LNE35_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBK6909586.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22904..23335
                     /gene="ruvX"
                     /locus_tag="LNE35_000399"
     CDS             22904..23335
                     /gene="ruvX"
                     /locus_tag="LNE35_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBK6909587.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23360..23665
                     /locus_tag="LNE35_000400"
     CDS             23360..23665
                     /locus_tag="LNE35_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBK6909588.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23746..24891)
                     /locus_tag="LNE35_000401"
     CDS             complement(23746..24891)
                     /locus_tag="LNE35_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6909589.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24952..25599)
                     /locus_tag="LNE35_000402"
     CDS             complement(24952..25599)
                     /locus_tag="LNE35_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6909590.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25793..26086
                     /locus_tag="LNE35_000403"
     CDS             25793..26086
                     /locus_tag="LNE35_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909591.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26130..26441
                     /locus_tag="LNE35_000404"
     CDS             26130..26441
                     /locus_tag="LNE35_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909592.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26438..27062
                     /locus_tag="LNE35_000405"
                     /pseudo
     CDS             26438..27062
                     /locus_tag="LNE35_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27203..27361
                     /locus_tag="LNE35_000406"
     CDS             27203..27361
                     /locus_tag="LNE35_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909593.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27362..27724
                     /locus_tag="LNE35_000407"
     CDS             27362..27724
                     /locus_tag="LNE35_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909594.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27761..28609
                     /locus_tag="LNE35_000408"
     CDS             27761..28609
                     /locus_tag="LNE35_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HBK6909595.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28599..30074
                     /locus_tag="LNE35_000409"
     CDS             28599..30074
                     /locus_tag="LNE35_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBK6909596.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30340..30747
                     /locus_tag="LNE35_000410"
     CDS             30340..30747
                     /locus_tag="LNE35_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HBK6909597.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30750..30965
                     /locus_tag="LNE35_000411"
     CDS             30750..30965
                     /locus_tag="LNE35_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909598.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30969..31349
                     /locus_tag="LNE35_000412"
     CDS             30969..31349
                     /locus_tag="LNE35_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK6909599.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31483..31638
                     /locus_tag="LNE35_000413"
     CDS             31483..31638
                     /locus_tag="LNE35_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HBK6909600.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31707..32180
                     /locus_tag="LNE35_000414"
     CDS             31707..32180
                     /locus_tag="LNE35_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBK6909601.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32177..33871
                     /locus_tag="LNE35_000415"
     CDS             32177..33871
                     /locus_tag="LNE35_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK6909602.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33837..34022
                     /locus_tag="LNE35_000416"
     CDS             33837..34022
                     /locus_tag="LNE35_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909603.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34026..35201
                     /locus_tag="LNE35_000417"
     CDS             34026..35201
                     /locus_tag="LNE35_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK6909604.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35194..36717
                     /locus_tag="LNE35_000418"
     CDS             35194..36717
                     /locus_tag="LNE35_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK6909605.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36773..37057
                     /locus_tag="LNE35_000419"
     CDS             36773..37057
                     /locus_tag="LNE35_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBK6909606.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37044..37379
                     /locus_tag="LNE35_000420"
     CDS             37044..37379
                     /locus_tag="LNE35_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK6909607.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37674..38300
                     /locus_tag="LNE35_000421"
     CDS             37674..38300
                     /locus_tag="LNE35_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909608.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39042..39879
                     /locus_tag="LNE35_000422"
                     /pseudo
     CDS             39042..39879
                     /locus_tag="LNE35_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            39944..40378
                     /locus_tag="LNE35_000423"
     CDS             39944..40378
                     /locus_tag="LNE35_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6909609.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(40446..41768)
                     /locus_tag="LNE35_000424"
     CDS             complement(40446..41768)
                     /locus_tag="LNE35_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBK6909610.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41922..43562)
                     /locus_tag="LNE35_000425"
     CDS             complement(41922..43562)
                     /locus_tag="LNE35_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBK6909611.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43892..44362
                     /locus_tag="LNE35_000426"
     CDS             43892..44362
                     /locus_tag="LNE35_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBK6909612.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            44991..46094
                     /locus_tag="LNE35_000427"
     CDS             44991..46094
                     /locus_tag="LNE35_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK6909613.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            46378..47058
                     /locus_tag="LNE35_000428"
     CDS             46378..47058
                     /locus_tag="LNE35_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK6909614.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47148..48182
                     /locus_tag="LNE35_000429"
     CDS             47148..48182
                     /locus_tag="LNE35_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK6909615.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            48196..48531
                     /locus_tag="LNE35_000430"
     CDS             48196..48531
                     /locus_tag="LNE35_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909616.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48515..50995
                     /locus_tag="LNE35_000431"
     CDS             48515..50995
                     /locus_tag="LNE35_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909617.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            51051..52610
                     /locus_tag="LNE35_000432"
     CDS             51051..52610
                     /locus_tag="LNE35_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909618.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52607..53971
                     /locus_tag="LNE35_000433"
     CDS             52607..53971
                     /locus_tag="LNE35_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6909619.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54142..55212
                     /locus_tag="LNE35_000434"
     CDS             54142..55212
                     /locus_tag="LNE35_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909620.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            55227..55895
                     /locus_tag="LNE35_000435"
     CDS             55227..55895
                     /locus_tag="LNE35_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909621.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55915..56493
                     /locus_tag="LNE35_000436"
     CDS             55915..56493
                     /locus_tag="LNE35_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6909622.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56896..57807
                     /locus_tag="LNE35_000437"
     CDS             56896..57807
                     /locus_tag="LNE35_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK6909623.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58063..58980)
                     /locus_tag="LNE35_000438"
     CDS             complement(58063..58980)
                     /locus_tag="LNE35_000438"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBK6909624.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59182..59619
                     /gene="zapA"
                     /locus_tag="LNE35_000439"
     CDS             59182..59619
                     /gene="zapA"
                     /locus_tag="LNE35_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBK6909625.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59677..60224
                     /locus_tag="LNE35_000440"
                     /pseudo
     CDS             59677..60224
                     /locus_tag="LNE35_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            60339..62699
                     /locus_tag="LNE35_000441"
     CDS             60339..62699
                     /locus_tag="LNE35_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBK6909626.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62786..63100
                     /gene="trxA"
                     /locus_tag="LNE35_000442"
     CDS             62786..63100
                     /gene="trxA"
                     /locus_tag="LNE35_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBK6909627.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63282..65060
                     /gene="uvrC"
                     /locus_tag="LNE35_000443"
     CDS             63282..65060
                     /gene="uvrC"
                     /locus_tag="LNE35_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBK6909628.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65205..66104
                     /locus_tag="LNE35_000444"
     CDS             65205..66104
                     /locus_tag="LNE35_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909629.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66097..67359
                     /locus_tag="LNE35_000445"
     CDS             66097..67359
                     /locus_tag="LNE35_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909630.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67522..69420
                     /locus_tag="LNE35_000446"
     CDS             67522..69420
                     /locus_tag="LNE35_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBK6909631.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(69719..70438)
                     /locus_tag="LNE35_000447"
     CDS             complement(69719..70438)
                     /locus_tag="LNE35_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBK6909632.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            70775..71107
                     /locus_tag="LNE35_000448"
     CDS             70775..71107
                     /locus_tag="LNE35_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909633.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71094..73097
                     /locus_tag="LNE35_000449"
     CDS             71094..73097
                     /locus_tag="LNE35_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBK6909634.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73116..73586
                     /locus_tag="LNE35_000450"
     CDS             73116..73586
                     /locus_tag="LNE35_000450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBK6909635.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73607..74191
                     /locus_tag="LNE35_000451"
     CDS             73607..74191
                     /locus_tag="LNE35_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBK6909636.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74206..75192
                     /locus_tag="LNE35_000452"
     CDS             74206..75192
                     /locus_tag="LNE35_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBK6909637.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            75182..75493
                     /locus_tag="LNE35_000453"
     CDS             75182..75493
                     /locus_tag="LNE35_000453"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBK6909638.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75515..77296
                     /locus_tag="LNE35_000454"
     CDS             75515..77296
                     /locus_tag="LNE35_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBK6909639.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77289..78665
                     /locus_tag="LNE35_000455"
     CDS             77289..78665
                     /locus_tag="LNE35_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBK6909640.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78672..79307
                     /locus_tag="LNE35_000456"
     CDS             78672..79307
                     /locus_tag="LNE35_000456"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBK6909641.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79337..80692
                     /locus_tag="LNE35_000457"
     CDS             79337..80692
                     /locus_tag="LNE35_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBK6909642.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80884..82278)
                     /locus_tag="LNE35_000458"
     CDS             complement(80884..82278)
                     /locus_tag="LNE35_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK6909643.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82370..83341
                     /locus_tag="LNE35_000459"
     CDS             82370..83341
                     /locus_tag="LNE35_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBK6909644.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83338..84120
                     /locus_tag="LNE35_000460"
     CDS             83338..84120
                     /locus_tag="LNE35_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBK6909645.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84289..84660)
                     /locus_tag="LNE35_000461"
     CDS             complement(84289..84660)
                     /locus_tag="LNE35_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6909646.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84794..85237
                     /locus_tag="LNE35_000462"
     CDS             84794..85237
                     /locus_tag="LNE35_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBK6909647.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85280..86089)
                     /gene="yidA"
                     /locus_tag="LNE35_000463"
     CDS             complement(85280..86089)
                     /gene="yidA"
                     /locus_tag="LNE35_000463"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBK6909648.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(86108..87475)
                     /locus_tag="LNE35_000464"
     CDS             complement(86108..87475)
                     /locus_tag="LNE35_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6909649.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87459..88289)
                     /locus_tag="LNE35_000465"
     CDS             complement(87459..88289)
                     /locus_tag="LNE35_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBK6909650.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88452..88889
                     /locus_tag="LNE35_000466"
     CDS             88452..88889
                     /locus_tag="LNE35_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBK6909651.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88965..89585
                     /locus_tag="LNE35_000467"
     CDS             88965..89585
                     /locus_tag="LNE35_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBK6909652.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        29552 a        15170 c        18472 g        26459 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt tataattaat gactattata acaaaaatga aaagccaaaa cgacaagtaa
     3301 aatatttttt tatataaata tttgtgttac taattccgtt aatatacata agtgaaagcg
     3361 ttttatataa gaacttgaaa agaaagaagg gatagacatg aaaaaaagat acaaattggt
     3421 gatagctttg ctaaacatta ccctattatt cagcttaagt gcatgtggaa aaactaacga
     3481 agaagtaaca gcaaacatcg cagatccaat gataaaaaaa gccactacat tagaagtata
     3541 tgtcgcaaac ggcaaactga caaactatgt tgaaaatgct gcaaaaatct ataaccgcaa
     3601 aactggagca aatattgact taaaaattgt gaatgtagct tcgggaacag caacagtaca
     3661 aatgatcacc ccaaaattgg taacacacga agatatgccg gatattatct ccatggcaga
     3721 cacttcagca gcaggtgtgc tggaaaaatt tcaagatgct ttttatagtg ctgatgaata
     3781 tggattttat gacaaatatg gcgatcagtt ttatgatcaa aaattgaata ttttgcgtca
     3841 acaaacgagt aatgaaacga ttattccttg ggctagtgat tttaccccag cactttgtta
     3901 ttatcaacca aaaaaatttg ctgaaatagg tgtagatttt gaagaaatca gttcttggga
     3961 agaatatatc caggtggcaa aagcattaaa agaaaaaact ggtagttttg gaattgcctt
     4021 gccagaatct ggggaccaag aattgtttat caacatgcta gcccagcaga atcaatcttt
     4081 gcttgatgaa gaaggaaata tccgtctgaa tactaaagaa gcgaaacatg ctgcaaagtt
     4141 aatcaaagaa atgatcgact cagatattgt acatttctac ggtgcacaag atgctgagaa
     4201 agcatttcaa gaatctgcaa tgtttgttgc tggcggctgg tatgctacga atatgtcttt
     4261 gaattttcct gatgcatctg acaaatggcg catggctcca ctgattcctt tttcaaaaga
     4321 aaatccagga aaaggtgcag tatcaggagg atcaagtttt tatgtcccta aagatgccaa
     4381 aaatccacaa gtggcacaac aatttcttac atttatgctg acagatgaag aatgcttagc
     4441 caacgcactg gagctgggcg tggctacatc taactcattg gcctatcaaa cagaagcagc
     4501 tgagaaaaag tttgactact aaggaggaca aaaatactat gaactattga ataaaatcaa
     4561 tgaaaataca tcaaatactg ttttaccagc cacttattca gatgctacgt cttatttgac
     4621 taccgctgca tataactatt ggcaaacagg tgattttgaa aattcatatc taaaagaagc
     4681 agaaaatttt gctcataagt acggcgtaaa attaactggt gattaaaaaa tattaggagg
     4741 acttaacgat gatgcaatct tcgttaccaa gcaagacaat aaagaacagc aagaaaaaag
     4801 caacagatac gtctcataaa aaaagtgctt atggattggt cacacctttt gttattttct
     4861 ttattgtgac gattttgatt cccatcatta tctcgtttgc ttttagtttt atgaaaattg
     4921 gctactcatt tgaatttgtt ggatttgaca attatctagc tatcttccga gatccattgt
     4981 ttattaaatc ttacacgaat gttttgatta tcatggcagg atcaattcca ataacgatgc
     5041 tattagctat ttactttgct gttttattga attcagcaca gttaaaagga aaaggatttt
     5101 ttagaactgt ctattatatt ccaaccatta catcaacggt ggctgtagct tccgtattca
     5161 tgactttttt caatccgact gggttattca atggattttt gaatgcgcta ggaattaacg
     5221 gtgtacagtg gttaacaaat cctttttgga tacgaatatc aatgatcatc acgatgattt
     5281 ggcttagtgt aggatataac actattttat ttttagctgg tcttcaaggg atttctaaag
     5341 agatatacga aagtgctgaa atagatggag catcgaaatt tcggcaattc tttttattaa
     5401 cgatccccat gctaaagcca atcattttaa tggccacagt tttagccact atcaatggct
     5461 tgggtaattt caatatccca aatatattct ttgggacttc caatggacca gaaaattcag
     5521 ctttggtcgt aggagtcaat ttatataaaa caagttttga aatggttgat tttggaaaag
     5581 cctctgctat tgcctggacg atggtgtttg tatctgttat attatcggct attcaattta
     5641 aattcggagg tgataaaaat gactaaacaa aagatacaaa gcagaatttt tacgtacgtc
     5701 attattgtgt ttggtgcctt aatatttttg atgccatatt tatatatgat tgtagcttca
     5761 acgcaaagta atcaagaaat tttaagtggc ggaatcaatt tcaagtttgg ggatatgcta
     5821 ttaaaaaact ggcaagccat catggcgaga tataattatc ccttgattat ttggaattcg
     5881 attgttattg ctgtaatcag tacagtttta gctacagcca gttctacaat ggcgggttat
     5941 gcattagcaa agtatcgttt tatggggaat aaactgttgt tcaatacagt tatgctatct
     6001 aggatggtgc ctttttttac cacattgatt cctttatttt acattatgag tcgtttagga
     6061 ttatcgaata cttatgcagg tgtgatcatc ccttcgattg ccagtacaac ttcagtgttt
     6121 atgatgcgac aatatagcag tcaatttcct acagcactga tggaggctgc cagaatcgat
     6181 ggtgccaatg agtgggtgat ttttacgaaa atcgctgtac cgattttatc acctacgatt
     6241 attactagtg gtttacttat ctttatggga agttggaatc aatatctatt tccattagtc
     6301 atgctttcag ataaagataa atatacggtt cccttaatta tcaaaaatat gacggtttct
     6361 agtacaggtg agccaattaa ttatggcgca attatgctaa ttttagcaac ttcagtgatc
     6421 ccaatggtct tgatttatgt gtgggcgcag tctaaattta aggagaatga cattggagct
     6481 gcaaataagt aaaaaaaata acgaatgtta accatctagt tcgttctagg agcaactcca
     6541 cattcataaa aattaaaaga atggcgggat atccatgaat gaacagacga aggaatggtc
     6601 aagaaaaatt ttgaatgtag cttctgaaaa gatagatgcg gaattagaca gaatggggaa
     6661 taaaattcca tatatttctt acaacggaaa atatacagac gtaataaagg acagaggaat
     6721 cgactggtgg accaatggtt tttggagtgg tattttatgg cagctctttc acaagacaaa
     6781 acgagaaaag ttcaaagagg cagcaataca acaagaacaa ttattagaat ctgccctgta
     6841 ccatttttca gacgttcatc atgatgtagg ttttttattc ttaccatcgg ctattgcaca
     6901 ttatcaacag tctcaaaaca aacatagtcg acaactaggg ttacatgcag ctacggtact
     6961 tgctggacgc tataatattt cagggaacta tttagtagca tggaatgacc ataaacctgg
     7021 ttggatgatt attgatagta tgatgaattt accattactg tattgggctt cgagggaaac
     7081 tggtgatccg aggttctcta aaattgctcg gcagcatgca gatacaacag ctaagtatct
     7141 agtgaggaaa gatggatcaa ccgggcatat cgcagaattt gatgcagaat cagggaaata
     7201 tattcggtta cttggcgggc aaggatacaa tgagaattct tgttggagca gaggaaatgc
     7261 atgggcttta tatggattcg cgttaagtta tcgttacaca aaaaatccgt actatctagc
     7321 tatagcagaa aagatagcag taaattttat tcagaaaatc gaaaaaactg gttatattcc
     7381 tctcattgat tttgctgtaa aagattcgaa tattgatacc agtgctggag tatgcgctgc
     7441 atgcggtctt ctggaattag caggatatgt ttcctctgta cgatcaaaaa tgtatcatga
     7501 agctgctatc aatatactcc aaaagacaga aaagcactat gcaaattgga atatggatga
     7561 agatgggatc attggaggcg gagcagaagc ataccataga gaactgactt ataatgtgcc
     7621 actgatctat tctgattatt tttttattga aggcttactt cgattagaac agtcggaatt
     7681 agctatttgg tagtaaaaag aggagaaaat acaatgaaac atccagtacc actattaagg
     7741 accagacagg attactttga cttatttaaa aagataaatc aaccactcca gccgttttat
     7801 cgagcaagtg gcgcacaaat tgattatata catcaagggg taggatatgg aaataaaatt
     7861 gcaggaatgg aaggattctc tcgggtttta tggggagcgg ggcccgcaat tgaccaatta
     7921 gatgaacgat ggttgaaatt aattctatca ggaataaaag ctggaacaga tccttcccat
     7981 cccgattatt ggggagaaat acatgaaaga gaccagcgta tggtagaaat gcccgcaatg
     8041 ttattagcgt tatatcatca tgaacaatta ttatggaaaa aattagatac aggagaaaaa
     8101 agccagatta ttcattggtt ctcgcaaatt tttgaatatg aatgtgcaga tggtaattgg
     8161 caatttttta agataattgt cggaacaatt atccaaaaat ttggttatcc agttaaagaa
     8221 tcagcactga aagaagcatt tgataatata gaaaattgtt atcttcaaaa tggctggtat
     8281 cgagacagtt caagaggtcg ccaagattat tataatcctt ttgcatttca ttattatggt
     8341 ttgatttata gcgtactaga accagaaaag cccattagcc agatatataa gaaccgagct
     8401 attgattttt ctagagacta tattcatttt tttgcggaag atggtgcaaa tgtgcctttt
     8461 ggtcgaagca tgatttatcg ttttgcagtt tcaagctttt ggtcagctat gttgtgggcc
     8521 gatcttttgc cttttaaatt aggtgaaatc aaggggttag tcaatcggaa tataagatgg
     8581 tggatgcaaa aagaaatatt tgatgaaaaa ggtatcctga atattggtta tacctaccct
     8641 caattgacat tgactgaacc ctataatagt acgttatctc cattatggct gaataagatt
     8701 tttttattgt tggcgctacc tgaagaccat gagtattgga cggctaaaga aatgcctatg
     8761 cctattcgtg aaggaactaa acttctttca gaagccaatt tgctcataca acatgataat
     8821 ggacatacat tttttttgaa tgcgggacaa ttagggccaa atttccatac attgaccaat
     8881 gaaaaatatt taaaatttgc ttattctagt caatttggtt tttcgatacc gcgagcgaat
     8941 caattgaaag cagaaatggc aatggattca atgctaggga ttcagcgagc ggacacatat
     9001 atcacgacca gttttaagaa tgagagcatc aaaacctatg gacagttcat cgttcgaaat
     9061 aatgtacgag acattgtatg taagaaagag gtacttgcct ctacatggga acctacttta
     9121 caggcaaaaa tcagaacttg gcttattcct tttgaaggct ggcagattcg cgtacacaaa
     9181 gtagaattag atacagaata tgttctatat gagacagggt ttgctattga atgttctcct
     9241 gagaaagaag gaataatcat agaagaagac aaggaaaact atagggtgag tgaagctggc
     9301 ttttcaggta ttatctgttt aagtgacgat actattaaac gaaaatccac agctattatg
     9361 ggtttgccca atacaaactt gatgacatgg gaaaacactt ttattcctgg attagagggt
     9421 acatttggaa aaggaaccca ttggctcgga acaggagtgg ttgctcatca agatcgggat
     9481 tatagtttag aagtatggaa caatcgtccc aaaattgatt ttgatgggac aaccgggcta
     9541 actatccaaa ataaaaacaa taaaaaaaga ctaaaattat gctaaaggct ggtggggaaa
     9601 tgcttgataa agtgatgttt gaagagataa tacatccaga aaaatggata aatgaatgtt
     9661 ataaagaatt aatggaaaaa gaatggaata atgagatcga gcgttgtcgc agacacacgg
     9721 agaggttaat caaacgtatc agtaacagtc agtatcaatt cccaagcgag tgtcctataa
     9781 aaggacgtta tccattaaga gacaatattg gttggacaga aggattttgg acaggaatta
     9841 attggttgtt atttgagaag acgggtgatc ctttatttca acaaacagct gaaaaagcag
     9901 atatagattt tatccacagg attgactgtc aaattatcac taatcatcac gatcttggtt
     9961 ttttgtattc tttatcaacc gtagcagatt ataaaataac gggaaatcga aaaatgaagg
    10021 aagcttcaat tgatgctgca gaaacattac ttcagcgttt ttgggagaag gcaggagtta
    10081 tacaagcttg gggagatcct agtgatcctt cacaacaagg tagaatgatc atggattgtt
    10141 gcatgaatct tccattattg tattgggcga gtgaagaaac tggaaatcaa aaatataaaa
    10201 aagctgctca ggctcatatt caaaatgcag cagattattt agtaagagaa gatggttcaa
    10261 cttatcatac attttatatg gatgtcaaag atggaaaacc aagatatgga agtacgcatc
    10321 aaggttatag tgataattcc tgttgggcta gaggccaagc atgggggatt tacggttttg
    10381 cattaagtta tcgttatttg aaagaacaaa aatattttga tttaagcaaa atattagcca
    10441 attattttat gaatcggttg ccagaagata ccatttgtta ttgggatttg atctttgatg
    10501 attctagcag agaagaaaaa gatacatcta gtatggtgat catggtttgt ggattattag
    10561 aattaataga atcaggtcta ttagacaaaa cagaaaagaa cttataccag tctgctgttt
    10621 ggaaaattat gcgacaattg aaagaaagat atgctacaac ggaagaagaa gatggctttt
    10681 taacacagtc tgtttataat aagcccaaaa ataatggggt aaatacacct tgtatttggg
    10741 gtgattacta ttatttagaa gctttagtga gaatgactag taattggtct tcttattggt
    10801 gaaaaaggta aatatggaat ttttgtttga taaaataaaa aaagaaaaac caaaacagca
    10861 tgaatggttt ctgttttggt tttttcgtta attttcatat tcttctaatt ccaagcttag
    10921 ttcttcccat tccgtcaatt ttgtttcttg atcttgtcta gcagcttcta aatcttggtt
    10981 cagctcattg agttttacgt gatcttccag gttttctggc gcgaccattt cttgttccaa
    11041 ctgatcgatc cattcatcga tccgcgccat ttcttcttca acctgagtaa tttttcgttg
    11101 aaggctacgg ataagctttt gctgttcttt gctttggtaa taatcagatt tgttcgtggg
    11161 agcaggagca gattctggct catcttgtgc caacaattcg gcgatttctt cttcttcctg
    11221 ctttttctct aaataataat catagtctcc tagatagact ttgctgccat tttcagagag
    11281 ttcgacgact tttgtcgcga tccgattgat gaaatatcgg tcatgggaaa caaaaagcag
    11341 tgtgccgtca taatcgatca atgcattttc taatacttct ttgttgtcaa tatctaaatg
    11401 gtttgttggt tcatccaaaa tcaaaaaatt ttctttgttc atcgctaact tggctaaagc
    11461 aacccgtgct ttttcaccgc cacttagcag agggattggt ttttctacat cttcgccaga
    11521 aaataagaaa gcacctaaag cacttcggat ctctttttct ggagtggtag gatgctcatc
    11581 ccacaattct gctaggatcg tcttgtttgt atgcagatca gcttgacctt gatcgtaata
    11641 gccgatggtg acatttgtcc ctagcgtttc ttttccacgg ataaaaggga tctggtgaat
    11701 gattgatttc agcaacgtcg atttcccgat accattcgga cctactagag caatcgcttc
    11761 ttgacgacgg atatctagat tgattggttc agataaggtg acattatcat atccgatagc
    11821 ggcatcttcg atttgcaaga ctacatttcc agaaggcttt tctatactga atagaaagtt
    11881 tgcttgtttt tcatcccctt gaggacgatc cagccggtcc attttttcta atgtctttct
    11941 gcggctttgc gctcgttttg tggtagaggc gcgaacaagg ttccgagcaa cgaagtcttc
    12001 taatttatgg atttctgttt gctgcttttc ataagccttc cattcacttg ctaactgttc
    12061 tgctttcaaa tctagatatt tcgaatagtt acctttgtaa tggcgcattt ttcttctgct
    12121 taattcatag acttcattga ctactttatc taaaaaataa cggtcatggg aaacaatcaa
    12181 taatgcacca gtatagcttt gcagataatt ctcaagccag gaaagtgtct cgatatctaa
    12241 atggttcgtt ggttcatcta gaatcaaaat gtcagggtgt tgcaaaagca tgcgagctaa
    12301 agctaaacgt gtgcgctgtc cgcctgataa agtatcgatt ttctgatcat aaaatgaaga
    12361 atcgaattta aacccatgca atacggaacg aatttcattt tcataaccat agccgtttcg
    12421 atctgaaaaa tcgtgttgca gccggtcgta ttcttttaaa taagaactat aagcaggtgt
    12481 gtctggcgta ccttcgctaa tgaaaatctc tagctcgcgc atccgctctt ccatttgtct
    12541 aacttgttca aaagctttca gcatctcgtt ccaaactgtt tcattggaat caagtcctgt
    12601 gtcctgagcg aggtatccga gagtcgctgt tttattttta gcgatctttc cttcatcagg
    12661 tgcttcaatt ccagcaatga tctttaataa agtcgatttt ccagcgccat ttctgccaac
    12721 taaagcgaca cgagcgcctg tactgatttc taaatgtatg ttttcaaata agacttctgc
    12781 accaaaatgg cgagcaatct gattggcttg taataatatc atggaatcct cttttctact
    12841 ttgtgaattc tcctcaaata aaaaactatt tttcaattag tagtctacca taaattccat
    12901 tatactgcga gatttgcaga aatgataaat aaatgaataa agaataaaat ttcacaatat
    12961 ctgagcaatg gaattgattt tttcctataa atactgctat gatggagaaa tcaaagatgt
    13021 aattagacaa tacgtggagg tcatttcgtg aaagatcata ttattccaaa ggcgacagct
    13081 aaaagactgc ctttgtatta tcgctattta aggattttaa ataatgctgg aaagaaaaaa
    13141 gtgtcttcaa ctgaattaag tgaagccgtt caagtagata gtgcaacgat ccgtcgagat
    13201 ttttcttatt ttggcgaatt aggaaaacgt gggtatggtt atgatgtaga agatcttatg
    13261 cacttttttg caaagacatt aaacgacgat caattgacaa atgttgcgct gatcggtgta
    13321 gggaacttgg gaagtgcttt actaaaatac aaattccatc aaagtaatag catacgtatc
    13381 agttgtgctt ttgatgtgaa tgaggaaatc gttgggcgca tcgtagacgg tatccccgtt
    13441 tatccaatgg aagatatgtc agaacaaatc aagatccaac aaatcgaagt agcaatttta
    13501 acgatacccg ctgaaagagc acaagaagta gtcaatcagt taacggaagc aggaatcaaa
    13561 ggaatcttga actttaccgc tgctcgattg aatgctccgt cagatgtatt gatccaaaat
    13621 gttgatttga cgaatgaatt gcaaaccttg atctatttct tgcattctga cgcaaatgcg
    13681 cattccgaaa atacagcaga ataaaaataa aaagcgattg aacaaatgaa ggcaaaactt
    13741 tcacttgttc aatcgctttt ttctgtttgg acaacgatta ttttgttcgg aagatttctt
    13801 cataagaaag ggaatcatct tctgctgtca gttcatctag tagataacgg atttttttaa
    13861 agtcgatgaa ccgtgcttgc cgaaagactt cttttccttg atgaaaaatc aggacaactg
    13921 gcgcagtcag cacttcaaat ttactagcta cttctggtgt ttcaaatgca tccaaatgaa
    13981 gtgccggaat atcataatgt gtgagaagtt cctctagtcg tggacgaaca gcgtgacaaa
    14041 cactgcaatt aggcatcgaa agataaatga cggcaacgga tgactgttgc agtatgtctt
    14101 gaacttcagt caagttagtg gtttgtttca tttttattct cccttttatg atagaagtga
    14161 tatgtatgag accttaattt aatgggaata acagaaaaag tcaattgaag tgctttggta
    14221 aaaaataaga gttgtgtaaa aatttacaga gcaacttgtt ttttttgaat aaaagcgtat
    14281 atttgcaaag cgaatgaact agaaataaaa aggaggaaac tcatgttttc ttttattcca
    14341 aattcggttt tgcagatgag tacgattttc ttatcgatcg ttattgaagc attacctttc
    14401 gtattattag gctgtcttat ctcaggcgcc ttacaagtat ttttgacccc agaacgtgtg
    14461 aagagatggc ttcctgaaaa tcgtatcttc tctattttta ctggttcgat cttaggattt
    14521 ttctttcctt catgtgaatg cggcatcgta ccgatcgttc atcaatttgt taaaaaaggc
    14581 gtaccagttc acacagcgtt tgcttttatg ctgactgctc cgatcatcaa tccaatcgtg
    14641 attttttcca cttttatcgc atttggcaat tcttggcaga tggcaagctg gcgggtgata
    14701 ggcagctttt ttgttgccct tgtgattggt gtctggcttg cctattttca aaaagacgct
    14761 gtattgaaag aaaaaatcac gtctaaagta gcagaaaatc accatcatca tgtccatgaa
    14821 gaagaacaaa gtgagaagaa tgaaccaaaa aatcagctga tccatcaaac tgggcatgtg
    14881 ttgacacatt caatcgacga attttttgat acaggtcgtt atttgatcat tggcggatta
    14941 atcgcagctg cgatgcagac ttatttacca actagaatta tgctaacgct cggctcaaca
    15001 aagattttag ccattatcat catgctgtta ttggccttta cgatgtctct ttgctcagaa
    15061 gcagatgcgt ttataggatc ttctttgtta agtctgttcg gcacagcacc tgtagctgcc
    15121 tttttggtct ttggcccaat ggtcgatatc aaaaatctac tgatgatgaa acgatatttt
    15181 acaggacgct ttattttatc gctgatcgga ctgattgccc ttagcgttat tggattcaca
    15241 ttatttattt aggagggaaa atatgctgcg gtttttgatt ttatctgggt atttcgaatt
    15301 gatgatgtac ttgcaagttt ctggcaaact cgatcaatat atcaatgtcc attatcgcta
    15361 cttagctatt ctttcaatga ttttatctgc tcttttggct ttagttcagc tgtatttgtg
    15421 ggtcaaaaac ggaaaagcag aagaaaaggc acacgagcac gatcatgacc atgggttgtc
    15481 aaaaccttat caacgtggaa tcgcttatgt actgctagct ttgccagtaa tcgtggggac
    15541 gttgtttcca acagttagtc tcgacactac gatcgtagaa gccaaaggat ttaatttccc
    15601 agtaagtaaa gaatcagtag gggatccgga gatgcagaca caatacttaa agccggatac
    15661 cagtatttat ttcaacaaat cagactactt agaccaaatg aatgagttga aagaaaagta
    15721 tggggataag caaactatca agatcacaga tgaaaactat ttagaagtta tggagctaat
    15781 ttataattac cctagcgaat tcattggcaa aaaaatcagc tatgaaggat ttgtttacca
    15841 aacaccgaac gatagcaata cagatatctt cttgttccga ttcggcatta tccattgtgt
    15901 ggcagattca ggagtctttg gtttgttgac gcatatgccg gaaggagtaa cggttaaaaa
    15961 tgatgaatgg tataaaatag aaggaaccat ccaatcagac tattatcaac cattcaagcg
    16021 tgaaatccca tctgtagttg ttacaaacgc tgagaaagtc tctgctccga aaaatcagta
    16081 tgtgtatcgt tctttttaaa agaacaaaat aaataaacat agctcagaca gctgacacgc
    16141 aaggacgtcg gttgtctggc tttttttgca tgatgtattc atttgtttga tatttatttt
    16201 agaagcagca tcctgaaagt atttgatatc ggtttcaaag gtgttggaaa agattgtgaa
    16261 gagaattaca atttattcct aacatgtatg ttgaaaagtt gtatgaaatg attttgatag
    16321 taaaatatcg agtggaggta ctttttatgg taacgcgtat atttattttg gatgatagtt
    16381 tggtttttga aaaaatgcta gtagaaattt tggaagaatc cagaatttta ttaagtcctt
    16441 ggaaatttga gatcgttcag ggattgaacg ttatacaatt tgttgaattc attaaaaata
    16501 atgaaatcta ttcctcagat atttttttcc tagacatcga tttgaacgcc tattatagcg
    16561 gtatcgaact aggcgaaatg cttagagaaa agaatgaaaa gtgtaaaatt gtttatctaa
    16621 cgtctcatcc gaataaagca ataactgtta ttaatcgaaa gattacgcct agtggttatt
    16681 tgataaaaga attcacttat gaaaaaaata aaaaaagcat ccaaagttac ttattgaaag
    16741 aaaaagaaaa ctatcctgtt caacatgcta cgaaaaaaag gtggacaact gtccgttatg
    16801 gtaatagcga tcactatatc cattacgatg aaatccttta tctaatgagc atgccaggtt
    16861 ataaaaataa aataggccta gtattgaaag atgaagagat tctcgtaaac ggcacaatta
    16921 aaaaatataa aaaatcactc gaatatgagt atttatgtaa agatttaaaa gcttatatca
    16981 ttaatactga aaacattaca tctattagca gagtaaacga aatagtaaca tttgcaaatg
    17041 gagaacaact agagatggga aagagaagta ttgataaact aaagaatttt atgcaagaaa
    17101 agatagaaaa tgaatcattt taaagaaaac aaagactcat atcttacctg ttagaagagt
    17161 gttttcctgt agaagagaac aacccaagta gcagcctagt cgtttctatc cttttttgtt
    17221 atgcactctt tttgacattt actccaccaa taacgaaggg tacaataaaa agtaataaca
    17281 aacaaaagga gtacagaaat gattgaattc atcaatgtaa gtaaaagtta cgggaaaaaa
    17341 caagcattaa aagacttgtc tctctcgata agacaagggg aaatttttgg atttttagga
    17401 cataatggtg ccggaaaatc aactacgatt aaaagtttag ttagtattat tgaaccatca
    17461 tcgggaacga ttttagtaga tggaatgaaa ttgacggaaa atcgattgag tatcaaacaa
    17521 aaaatcggtt atgtgcctga ttccccagat atttttttac aattgacggc tggagaatat
    17581 tgggacttga tttcagctgc ctatgaatta gacacacaaa aaaaagaaaa gcgtttagca
    17641 gaattgacag cgctttttga tatgtatagt catcaaaatg aaacaatcgc aagtttttca
    17701 catggaatgc gtcaaaaaac gattttgatc ggcacgttgc tgccagatcc agatatttgg
    17761 gtattggatg aacctttgca aggacttgat cctcaagcag catttgattt gaaagaaatg
    17821 atgaaggctc atgccgcaaa aggaaaaaca gtcatttttt ctacacatgt attggatacc
    17881 gcacaacagt tatgcgataa actggctatt ttgaagaaag gtgaattgat ttatcagggg
    17941 gcagttagtg aattattgaa tggctcacct gatgaaacgc tagaaaagat ttatttgaaa
    18001 atggctggaa ggcaagcgac aggagatgga ccgtatgtat aagcgacaat tattagaatt
    18061 aattcgagtt aatcttttat atgttaatcc acagctcacg aataaagcaa gagagaaagg
    18121 aaaaaaggga aagcgattaa ctatctatct tttgtctcaa aatctttttt cagggctgat
    18181 ttttttactg atttacggat ttgctatgtt tgctatgaac tttgttaaat tgccaggttt
    18241 ctttacttat tacgtggcac tttttggtat gttaggttta actcaaagta tatcagtcat
    18301 ttataatatc ttttttgaag gaaatgacct gcaagtattt ttgcctctgc cttttggaca
    18361 agaacaggtt ttttcagcaa agattttggt agttgctttg accatcattc cttttgtttt
    18421 tccgcttcta gtcatgttta ttttaaccgg atggcgagct gatctaggga ttttgcttac
    18481 tatcattatc tcaatccttt tgttttctct atttttgatt attctttttt gtctggctag
    18541 tttgattgtt tttggtttgg ctcgaacagc agtgtttaaa aaacatagaa aaacagtttc
    18601 tagtatcttg cttggcagct cgatgctgat cgcatttgct ggaatcatgt ggatgaatca
    18661 tcaaacgggg actattgaaa atgagttaac agatcggcat ccgatttcga tattattgcc
    18721 attctatgat gctgccagtc agccactttc tcaacaaggt ttattgggtt ggggaatact
    18781 agctatcacg gctctgggat ttttccttgc tgtaaaacag ctgatagtac caaagcttac
    18841 agagcaactg actgattcaa acagtgagtt caaaataaaa agaacccata aaaagaacca
    18901 aaatgtgcat cagcttttat ttagttacaa tgctcaatta gtaaaagaac ctaatttgat
    18961 tatgcaagtt ctttcttctt ccttgttaac tccaatcatt tttattcttg cttttgccct
    19021 aggaggcgaa ataaaattaa ctgatttaga taataagtta atcggtgttg ttttcctggt
    19081 agggatcgca ttggctttct taacagttaa tcagacttct tttatcagca atcttatttc
    19141 tttggatcaa gaaaattttc tgtttatccg ttctttgcca atctctatga accaatattt
    19201 aaaagaaaag ttcagattcg gatggatatt gcagagtttt ttaactggtg ggatcgcatt
    19261 attaagcgga ctgtcttttc agctgccttt gttttttatc cctagcttgc tatttggttc
    19321 tttattcggc tgctacttat tgtcactacg ttatttcgct agagattatc ggttgctgct
    19381 gctgaactgg accaatatta accaattatt taacagaggt tcaggaagtt tgggtttagt
    19441 cgcaactatg atgggttcat tgatcatcag tattattctt atcgtcttat acggaatgtt
    19501 ggctttgttt tatccatttt ggttagtgaa tattcctgtg atcggtttag ttctaatact
    19561 ttccgctcta tggatcgttt attatcaacg aacgttttgg aaaaaatttg aataaagaag
    19621 aggggccgtg acaaaaatca tgtcgcagcc ccttttttac agatagaggg tgttcaaaag
    19681 tcagtttctc agtattaagt caattttatg taaaaatgtg agaagctatt tttacaaaac
    19741 tttttttaat actcataatt acttcttatt gctcctgcgg ttacttgtcg caatgtttgt
    19801 gagatatttt gtcatcgctc aaggctgaac gtctctttca caaccttttc tttgttacac
    19861 ttcgtttatt taaaaagccg tttttttcgt ttcatcatga ttgcgtcacg ctttactttg
    19921 aaattacgga tgttatggtt aaagacttct gataatacaa gacctaaagc gattgctcca
    19981 gcaatcataa ttgcttggac cgcataatga gccgcttctc gataggattc agtaacgaga
    20041 tttcgaaccg cttgataagc taaaccacca gggactagag gaaccatccc gggaatattg
    20101 aaaatcgtta cgggcatttt caaacgttta gagaaaagat cgctgatgaa tgctactcct
    20161 aaagaaccaa gcaatgaacc aaaagcagca gatccgccta tttgaacaca gatccagtag
    20221 atcatccagc caaaagcacc agaaagacca caacaaatca atgaacgcct aggtacattt
    20281 gtaataacgg caaatgccgc agtcgctaaa aaactaaaag aaaactgaat aaatatcgtc
    20341 cccatacttg tgttcctttc ctaataaaaa agctggaaaa taaaagcaat cgcaaaaccg
    20401 atcatcgtag cagtcatcat cgcttctgtt ccgcgagaaa ctcctgagac ataatgtcca
    20461 gctagcaaat cacggatagc gttcgtaatt tgaactccag gaactaaagg catcacacat
    20521 ccgataatga ttagatcctg attgcttcct aagccaaaac gtacgctgta gatagcggct
    20581 atgccaatca ataaagaaga taaaaattcc gataaaaatt tgattctaaa aaatttcaaa
    20641 ctatatagat aagctgcgta tcccaatcct ccaatgacta aagtcacagg taaatcggtc
    20701 atctttccgc caaataacac cataattgtt ccactgatca ctgccgcgct aacgatttgc
    20761 aaccagacag gaaagaaaga acggtctcgg tcgattgctt gcagttcgca gtaaagttct
    20821 tctaatgtta gctctgaagc aacatattta cgtgaaagat catttacttt tactactttt
    20881 tctaaattaa tcgaacggtt ggtaatttgt tccattcgga tggtcgaggt acgatcaaga
    20941 ccaataaaga gaccggtttg agtgacatag ctgaccaaac ggaaatttcc agaagctaaa
    21001 gcaattcgac tcatcgtatc ttctacccga tacatctccg cattgctttc catcatgatt
    21061 ttcccagcga gtaaacaagt gtccagcaac agatctaatt cttttcccgt catagcatct
    21121 tctccaataa ttgattgatt agttttattt tactacaaaa cataccagaa gcgtaaagaa
    21181 taaaaaaact tgaggaagga ataaaagagg cgtatactgt ttttgtattg cgggtgtagt
    21241 ttagtggtaa aatcccagct tcccaagctg atgtcgcggg ttcgattccc gtcacccgct
    21301 taaaataggt tgtgaaaaag ctgctctgca acaagtaata agaacagcca agcaaaaaca
    21361 acttcttatg tttttaactt ggatggactt attaccgcag atgcaagctt ttgcgcaccg
    21421 tttaccaagc ggttgtgaaa tcagtgtatt gtcttgagta tgaagaaaat ttttgaataa
    21481 taataaatac cttacagata gagtggtgac tgattagaga cgaggaatcc gtctcttttt
    21541 tgtattttta agccatctta aaacaggttt tattttttgc tgtttctatt tctttctagt
    21601 atttatttat aggggaacat atgttaagat ttagggagct gttagtaaag gagtaaagaa
    21661 atgagcaaaa aaacaaccaa ggcgatcgtg gggatcgtcg ctgctgtgat tgcaggggca
    21721 ttcggtgttt ccaccacaca aaaagattca gatatctttc agcagattac aactattttt
    21781 gattccacac cacaagcttc tcaagtagcg gatgtgccag aatatgatgg aaaacatcaa
    21841 gaaatagaaa tcaatgggaa tcagccggaa tttacaaaag aggatcttag cctttccaaa
    21901 ggaacatggg aaagctattc aaatattgat aaattgaaca gagtaggaca agccaatgct
    21961 atgcttggaa aagatttgtt ccctactgaa aagagagagc ctttgtatat tgacccgaca
    22021 ggttggaaac aaaagaaatt aagtgacggg caatggttat ataatcgcag ccatttaatt
    22081 ggtttccagc tgactggtca aaataacaac attaaaaact tgatgacagg gacacgttcg
    22141 ttgaatgctc catacatgct tgctcacgaa aatgatattg cggcatatat caaagaaaca
    22201 aatcatcatg tacgctaccg tgtcactcct cattttgagg ggaatgaact ggtagccagg
    22261 ggagtgcaat tggaagcaga aagcattgaa gacaaaaaaa ttgaattcaa tgtctttatc
    22321 tacaatgttc aagatggcta cacgatcaat tatgagacag gacaagcaac aaaaacaaaa
    22381 aattagtagc agctattcga attttttatg aatattatga aaaaattgtg taaaaacata
    22441 cattcaaaga aatatagcgt atactttatg aaaagagtac ttttgataaa gctacagaaa
    22501 aaaggaaaaa aacatacaaa tagtgtttct taatttattc ggaagtttgg aatatttgtt
    22561 tgtagttttc cctttcttcg agtaaaataa ggttgtagaa ggtaagctgg aattaccttg
    22621 cgggaaagag gtgtagaatc atgggtttta cagatgaaac cgtacgtttt ggttttgacg
    22681 atagtcgcaa aaaagaagtg agcgaaacat tagcgattgt ttatcgggct ttggaagaaa
    22741 aaggctacaa tccaatcaat cagattgtcg gctacttgct ttctggagac ccggcctaca
    22801 ttcctcgtta tcgtgatgct cgaaatttga ttcgccgtca cgaacgcgat gagatcatgg
    22861 aagtaattgt caaggactac ctctcaaatc atggagtgaa tttatgagag taatgggctt
    22921 ggatgtcggt tctaagacag taggtgttgc tatcagtgac ccctttggct ggacagctca
    22981 aggagtagag atcattcgga tcaatgaagc agaagaagag tttggctttg atcggttgaa
    23041 ggaattagta gcacaatatg aagtagaccg gtttgtagtt ggattaccta aaaatatgaa
    23101 caattccatc ggaccaaggg cagaagcttc catgtcatac ggagaaaaga tcaaagaact
    23161 attctccttg ccagttgatt atcaagatga acgattaacc actgttcagg cagaacgtat
    23221 gctagtagag caggcaaata cttcaagagc caaacgaaaa aaagtcatcg ataaattagc
    23281 agctgtgatg atcttacaaa attatttaga cggctcaggt aagcaattat tttaataata
    23341 aaagaaaaga ggtaatctta tgacagaaca taaccattcc cacgaccatg aaggacacga
    23401 acacatcaca ttggtggatg accaaggaaa tgaaacatta tatgaaatcc tactaacagt
    23461 tgacggacaa gaagaatttg gacgtaacta tgttctttta tatcctgctg gtgtttcaga
    23521 agatgaagat gtagaattac aagcttatgc atacatcgaa aacgaagatg gaacagaagg
    23581 cgagttagaa caaatcgaaa cagacgcaga atgggacatg attgaagaag tattcaatac
    23641 attcatggcg gaagaagaag aatagactat aaagctagga gaaaccttga tatgataggg
    23701 tttctccttt ttttattttt tactgtatgc catattgtat gccatctagt caatatttaa
    23761 gtgttgtact aatttttcaa cagtcttttc tttcgtttct tttgccaagt gaccgtaaca
    23821 atcgatcgtc tgactaattt ttgtatgccg taatctagtt tgaacttcta ccatagtagc
    23881 tccagcgtcc aagagcattg aagcatgagt gtgtcttgct acatgcacac cgagaaatgg
    23941 aaccatagct ttttcacaaa tagtaatcaa tcttgcataa atagcgtggt gtgtgattag
    24001 ttctttatag ataccacaaa aaatcatagt cggatcagtt acaccaagtg tcagcatata
    24061 tttccgctga ttaatctgcc atttttttaa tatagaaatt gttctttcat ctagtggaag
    24121 aaccccagca ctacaatcag ttttaggatc actgatttta tagccatttt tggattggct
    24181 tagtgtttta ttgatggtga ccgtattttc ttgaaaatca atgtctgacc aattcaatgc
    24241 aagggcttca ctgatcctaa ccccgctata aaagagaaag cgtagtaacg ttttatcaaa
    24301 gtcgtttcga taagtttcag gtttgctgtt taggtaatca aacaaggtga tcagttgttc
    24361 tttactgtaa actttgacct tttctttatg cttagtggtg gttcttttag gcattaatac
    24421 gttgtctagt ggattagtct caataatccc aagattgata gcgtagctaa aaatacgctt
    24481 agaagttgaa tgaagtacct tgtattggtc agtagtttca gcccagctat ttacaatttg
    24541 ttgggctttt tttaccgtga cttcattcac acgaagattt ccatattttg gaagaatcca
    24601 ctttctaaac ttgatttcag ttttcatgaa ggtggcttct cggacagtgg ttttatatgt
    24661 ttctagccac aagtcataaa cttctttaaa cgtttcgttg ttcgcttttt taataccgtt
    24721 tttatcgtag tccacttgca agcgtgacaa ggcaagctgg gcttcttttt ttgttttgta
    24781 acctcgtcta cgaaccttat caggtttgcc agtcattttg ttcattccaa gataggcttc
    24841 atagagccaa aatttacctt ttttagtttc atattgcttg aatgttgcca taatagtttt
    24901 ccctccatag tgtagcaagc cgtgggggcg gttggaggag gcagtttatt attattttga
    24961 atagtgttta tttagctcag taacaagagc tgtgaactct tttagaagat cattttcata
    25021 aaattcttga tactcttttt tctcatctgt atttttttct gaatcattaa ttaatgagtc
    25081 atatcgtaaa agcgtattta gaagagctgc gagataagca attggggtac tcccgtcagg
    25141 aaattcaatt cgagatcctg ttttaagata ttttattgcg tttgttaaat aaatcttctg
    25201 tgagacagtc aggttatctt cttcgatgtt attgatagca ttagaaagat tagaatcaat
    25261 tatagagtta tttttttcaa tagttttttt caattgcttt tcaagcacat ttcgcttaat
    25321 ttcgggcatc atatgcatat ctcttaatgt ataagatccg tttaatagat aggtagtgga
    25381 tacatctaat acctttgaaa gttgtttaag cctggtgtta ttgggaagat tatagccatt
    25441 ttcccaatta ctaacggcac ctttactggc cggtggggat aatttacctc caagttgttc
    25501 cattgagagt cccttttcaa ttctagccag tctaattctt tgtcctgtct ccactgcttc
    25561 ggggtatttt tttctagcca cattttcacc accattcata tattttctct actatatcac
    25621 gatttaaata tgtgtgaaac ataaaagttc aaaaaagtat gttaaaattc ttgcaaatga
    25681 aaaggtatga tggtatattg agtttataag aggtatagaa atgtattcaa gagcctcttg
    25741 cattttttta aaatctttgt atagaaatgt atgctaacga ggggggataa atatggaatt
    25801 aaaaattgac aaactcacgc ttgatgatcc agcttttaaa attattgacg atcgagttgt
    25861 tcaacaagta gagcaaactc aaggaacgtt actttcatta ttagaaaaaa agtaccagtt
    25921 tccaaaatat atgaacaagg cacaagctgc taagtatatg aacgtttcat acaatactat
    25981 gatgcagaaa tatgttccta acggtttaaa gttgattata gttgatggag tcgtaagaat
    26041 ttcacaagat gaatgtgatc gctttatgga agaataccaa aaataaacgc aagccgtggg
    26101 ggcaaaggga ttaataggag gaaataataa tggaatcgac acaaaataaa gcaatcgaaa
    26161 aagtattatc aacaattctt acagaagaca cagcggcaga attagcaaac ttggaaggca
    26221 aagcgctaga agaaacgttt gaatgtttgt acgaacaaat ggactatcaa aaattgctac
    26281 cacaagaacc aacggtaagc ggagtattgc gtggcttgaa cgatttagta caagcagagt
    26341 ttaaagaacg actttctatt gaagagtatc aagaaatttt gtatcagcaa gttgagttgt
    26401 tagcgagctt gctagggctt gaactggagg acgttgaatg aaaactgaaa tttggaacgg
    26461 acacattata agatttgtcg atattaatga tgaatggtgg gcagtggcga aagatgttgc
    26521 ggaagcgtta ggtcttaaac aggttactag agccattcac tctttgccta aagatggggt
    26581 tactactagt aaggtcatcg attcattggg cagaacacaa gatgtaaaca tcatcaacga
    26641 aaaaaaatat ttaccgcatg gcattcaaaa gccgtaaaaa agaagccgag gcatttcaag
    26701 actggatttt cgacatcatc aaagaactac gccagtccac aggacttgaa ggctttcaag
    26761 tattccgcat gctagacaaa gaacaccaaa aagaagcaat gactaaatta agccacgcta
    26821 ttactgaacc aaaaccagta gactacatta aagcgaatgt gatagccaat aaagctgtat
    26881 caactattta tggtcattcc aaaatggtaa aaaagaaaga tatgactcct gaaatgttgg
    26941 ttgatcgtga acctatttta gatgagacag tagaattaat gactgttaaa gaaaaatatg
    27001 ggttgcagtt ttcagtgagt gagaaaattt ataatcgttc tgctgaattg cagacaactt
    27061 aggagcatta acatgatcga gaacaaagaa aaagccttat ccataacagt cttggcggac
    27121 aggataaagg caacttaatt gggataaaac ccgatattct ttgttccgat tataccatat
    27181 taaaaaatcg gagggaacca ttatgaaacg aaacttaatt ataggaagca ttttaatcgg
    27241 cggaattttc ggcttattac cattaattgc aaaaatagct atctttgcag gattagcgtt
    27301 atatctcttc actgcttatg acgagtacga ctatcaatta agaactggag ggcataagta
    27361 gatgtacaaa acaaaactat taaaccaact cgatagcctt gaattagagg aaattaatca
    27421 agggatcgct gaactagaaa ataatatcgg gaaaacctac tttggaaatt cattcaatga
    27481 aaaactaaca gttttgtatg tactcaaaaa acatgcagaa cacaaaataa tttgtcgaga
    27541 aatcaacgag ctaaaaaatc agattttaac cgcatggtta aacattactg atatgcaaga
    27601 agcaagggtg aaaacattta atacatgggt aaagtatcag aatcaattaa aaggtgctga
    27661 atttgttcgg gatgggttga aatacgaact tgagcagttg aagctcatgg aggtgtctga
    27721 atgatttata ccagtatttt gaaaaaggag gagaggttta ttggcaaata attatcaagc
    27781 agcaacggaa ttacaaaagt cgggatggtc aatttatccc ttatctcctg gaactaaaat
    27841 tccaataaaa ggctctagtg gttggaatga tgcaactaac gattctaatc aaattaatga
    27901 atggtggacg gaagatgcaa ctagaaatat tgggttaatg cttgaagatc atcaaatggt
    27961 ggtagttgac ctagatcaac attctgaaac acgaaacggc gttgagaatt ataaaaaact
    28021 tgcttcaatg tatgatccat ttccttctac ttatacggaa atcacaccag gtaaaggaat
    28081 ccatttcttc tttaaaattc ctgatggtgt aattatccaa caagaaacag gtgcatttgc
    28141 tgatatcttc gggcgtgatg ataaaggtaa aagtttaagc gggattgata ttatcactaa
    28201 aggagttcca atagcgccaa ctatgattat ttcaagaagt gttgggagag catatgaacc
    28261 tattgaaaac gtatctctaa ataatattgc accagctccc gattggctag tgaacctact
    28321 gctaaaaaaa ccgcaagaaa taaagactca ataccgaccc gtagcaaaaa cagggacagc
    28381 aaaaaaatta gatatgattg ttcaaggcgc tggcgaaggt agtagaaacg atcatttaac
    28441 taagttatgc ggttggttac tatggcacgg tgtagataat caaactttag cagaattgat
    28501 ttatacagca aatgcgtaca atgaaccacc gctaaaagat aaagaagtga atcaaattat
    28561 tcgatcgatg attaaaaaag atttgagggg gaaagctcat ggctcataag cataaattag
    28621 atagtatttt agactttccc gaagccagcg agcgtgaaga caatatcatt gaattgaaaa
    28681 cctggatgtc acgattaaga tgcaataaag acgatcagat taaaagtaac agtgttgtga
    28741 atgcagaatt gattttaacc aatgatagca atttagctgg aacaattgct tataacgaat
    28801 ttagcggata cattcaccta ttaaaagatt ctccttggat caatcgtacc gctggagaat
    28861 gggaagacag ttttgaggat gccttaacgg catatattga agaaaactac aatgtagttt
    28921 ttgatgacaa caaaatacac aaagcagtag taaatgtggc tagaaagaat gttttcaacc
    28981 cagtaaaaga acgtatcgaa aaagttaaat gggatcaaca gcctagactg gaaacactgt
    29041 ttattgacct tttaggggta gacgacaacc tttacacacg agaagtaaca aaacgttgga
    29101 tagtcggatc agtcgcacgt atctataaac caggaataaa gtttgagatc gttccagttt
    29161 tggatggacc gcaagggatt ggtaagagta ccgttccagc attactttac acggatgatt
    29221 ttttcaccga ttcattagat tcgctaggtg aaaagaaaga tgattatatg cagcttcaag
    29281 gcaatgtaat tattgaactt ggagaattat cttcaatgaa caaaactaaa attgaacaga
    29341 taaaaaactt catcagtgcc aagatcgata aaattagacc tccttatggt cggaatgtca
    29401 tagcttgggc aaggcaatgt gtatttattg gaacgtcgaa tgatgggcag tatttgaaag
    29461 atgatacagg aaaccgccgt ttttatcctt tgccttgtaa gaataagcca aagatgaatc
    29521 cgtttaaaac aaaggatgaa tattttcttc aagtgttagc tgaagcaaag gtgctttttg
    29581 ataaaggaca acgaatctat tttagtcccg atgaagataa agaagtctta gaaattgcaa
    29641 aagactatca agaggatgcc aagatggaga atccaattaa agaagcaatc actaaatatt
    29701 tggaaatgga aatcccgtat aaatgggaag aagctccagc gtgggcgaga cgttcttatt
    29761 atcagaacta tcctgacagt aaatgtaatg agaaaaattt acagcatttc tataatggag
    29821 ctactatgca acaagagttt tatttagttg atagcgtact caccgctgat attttggaag
    29881 ctgtttttga taaacaagca aaggatttat tgaatggtcg atcagatgca gaaactaaaa
    29941 agattgcctt gattattagc agtattcctg gttgggaacg gaagcaactg ccaaaaagaa
    30001 ataaacgacg aggattttac aataagtcaa atgcctatga aaataaaaat cgagctggaa
    30061 agtacaaaaa gtaacgggta gtaacgggtc agtaacacct agcccgttac tagtttaccc
    30121 ttactgcccc aagggataca gcaatttggt aacggggtaa caccttattt ccttaaaact
    30181 tttgaaatag ttagctaata tgctggggaa taatgttact ggattttacc cgttacctgt
    30241 taccaaaacg ctagaaccct tgtccccgtt gggattgagt ggtaacgggt acctgttacg
    30301 taggtgttac cacgtgttac gaatcaagga ggaaagtaaa tgctcaatat tatttcaaca
    30361 aacaaagcac cgaactttca atatacagat gaaatggatc ggtttctaat gaacacgctg
    30421 gcatttagtg tgggattggt aacagaagat tattccacat ttgatccaga agtattaaag
    30481 ataatggaag aagaacctga ttggctgcag gagtcagtag cgtggtgtca atcgctagta
    30541 gtgggatcat tggtagatag tggcaactat gatgatacag gtgagctaat ggatgaattt
    30601 aattgtctgc ttaacctata tgatcgagcg agacaacgag agcttacatc gaatgaggat
    30661 aatttgtttt taaacatcca tgataagttc ttggctttgc tactaacaga tgatgaattg
    30721 ataactaatt tattggaggt agaataatta tgcatatgga aagtattaga tacttcgatg
    30781 gtgagaaaca cattgagtat tcaaacactc aagaagatgt agactttatt agttttgagg
    30841 aagataaaat cactaaagtt aattttaaag acggaacgtg cttgaagata gtttcgcctt
    30901 atattgaata caaatcaaaa tgggaacaag atgattctga cgaacgagat cgctttggtt
    30961 ggtaatctat ggcagttaaa aaacaatgta atcatgctgg atgtaaaaca ttgattgatt
    31021 ataggcagaa gtattgtggg aagcataaag caaagcaaac ggcaattaag cgtgaagaaa
    31081 gaaagcaaag cgaaggtaaa tattttcaat tctatcaaag tagaacatgg cgaaaggctt
    31141 catacttgta tcggttaaat catccagtat gtgaggattg cctagaagaa ggcttgataa
    31201 ggaaagctga tgttgtagat cacaaaattg aactaaaaga tgattggaat agacggttag
    31261 atgaaactaa ttttcgttct ttatgccatg cacaccataa ttccaagaca gccaatgaaa
    31321 gacaaagacg agaaaagagt accctttgag ttaagggggc atagtgtgaa acttactgac
    31381 aatcgatgcc tactcatttt ggtacaaata accgttgtga aaaggcataa gggtaattac
    31441 aaataacgat tacacttgta atcacaagtg aaaaagtata taatgaaagt aggagattta
    31501 ttgaaaaata caattgttat tactgataaa acagggaaac agcgcaccat tgagttacct
    31561 cattttggtc aagtgaccat tcaaatgcag aacggaaaaa tcatttatat agataaatta
    31621 gataaagaaa aattctgatc gaaaaacgaa ggaatgtgag cttaattgct tacgttcctt
    31681 tttcttttgt ctgaaaggag gacaacatgg gacaccctaa aatattacac gatacaaaag
    31741 gcaatatatc aagtgaagaa aaagcaatcc gtgtggacgc tagagaagaa ttgttcaagc
    31801 agcaaccatt aataaatatc acgccccctg actggatggc agcgagcgct agaagtgaat
    31861 ggaatcgtat cgtaccaaca ttaaaaaaag attatccatt gagtgaagca gattatggtt
    31921 cattggtagc ctattgttta gcctttgctc gaatgaaaac agccgaagcc gagataagaa
    31981 aatcaggtac gtttatcaca tgcgaaaacg gagtaaagaa agccaatcca gcggttcggg
    32041 ttcaatcaca agctatgagt gatttgaaaa aacaagccac ctcactaggt atgaccttag
    32101 aatcacgatc aaaactagct ttgaacaagg ttaaaaatga tgaacccgaa gacccgttca
    32161 aagagttgat gaattcatga atgattacat tgagaaagtc ctatcaggta agctgattgc
    32221 acccaagaaa attatccaag cgtgtgagcg tcatataagc gatttggagc gttctaagtc
    32281 agatagcttc ccttatgtgt tcgatgaaga acaagccacc aaagcgatta aattcattga
    32341 gttgctacca tctacggacg gtaaagcaat caagatgtta ggatttcaaa aattcattct
    32401 aggcagtctt tatggctggc gtactaaaga agggaattac aggcgattca atcgagcgtt
    32461 taccagtatg agccgtaaga atgggaaaac gtatatcgca agtggcatgg ctgccaatgc
    32521 gttgattatg gaacaagaac cagcagaagc aaggcaagta ttgtttgtaa gtaacgcttt
    32581 gaaacaagct aaattgggct atgatatgct gtctaattca cttagaaacg tggtcaagtc
    32641 tagtaagttt ttaagaccac aactgaaaat tatgaactct aagattcagc acttgccctc
    32701 taattcgttc gctatggcac tggctagtga aaccagcacg ctagatgggt ttgcaccaac
    32761 aaccgcaatt cttgacgagt ggcacgaagc aaaaactcgt aaaacgtaca acgtcattaa
    32821 gtcaggaatg acccaacaaa agaacggctt attgtgtgtt attagtaccg ctgggcttga
    32881 tttaaacgtt cctatgtacg aggaatactt attgttagat cgtgtgctaa aaggcgaaga
    32941 acaagccgac aggtacttta tagcgatatg ggaattggac gatcccgaag aaattcacga
    33001 tcaagagaaa tggatcaaag ccaatccgat ttttgaaagt gaagaaatca aaaaagtaat
    33061 gattccaacc attcaagatg atgtgaacct tgctttgaaa caaaataacc ttaattctgt
    33121 attggtgaaa aacttcaatt tatggagaca agcgagtgag gacagctata tgattgctga
    33181 agactggcag gcaaccgaag tagaaccaca agatattaca ggcaagcccg tttatatcgg
    33241 agtggattta tctaaaacag atgatttaac tagcgtttca tggatcgtac cgctagataa
    33301 cggcgaactt tattgtgatt cacatagctt tgtagccacc aaatatgggc ttcaagacaa
    33361 agaaaagcgc gacggtttgc cttatcgaga actagaaaaa gctggtgagt gttccattac
    33421 tcaattagaa agtggaatcg tggactatga ccaagtattt cagtttattc aagatttgat
    33481 tcaagaaaat gatttggaat gtatggggat ttgttatgac ccgtataacg ctaattcgct
    33541 tatcagtaaa gctgaaaaag ccaactaccc aatgttagaa gtaagacaag gaacgattac
    33601 tctaaacgtt ccgacccgaa cttttagaga acaagtttat gaaggcaacg ttattcacaa
    33661 taaaaatacg attctcaccc atgcagtgaa caacgctatt ttaaaaacgg ataacaacgg
    33721 cattcagatt aataaatcaa agaacagtaa caaaattgat ccaatagctg cattaatcaa
    33781 tgcctatgtg tttgcaatgg attacttcac cacaacggaa ggagcgaaag cagacaatga
    33841 attttataca agtgaagaat tttctttcta attacattca taccgttctt ttacttcttg
    33901 gattggtgtg tgtgttggtt gctatcacct tactaacaaa tgtctactat ggcttgttag
    33961 cgctgggcat agtgcttatt ggggtagcgg tcatgctaaa tacagaacag aagggaggtt
    34021 aaaagatggc attttttaaa gcgagacaaa atacaacggg agatcctttc ctacaacatg
    34081 tggtatcaat ccaatcggat gattacacca ccagctttac aagcgttcgt gcattaagaa
    34141 atagtgatgt gtttgcagcc gttcgaatta ttgctagtga tattgcttca agtccgattc
    34201 aattggtcaa aaacaatatg ccacaagctg ataatgaact ggtgaagtta ctaaacgaga
    34261 aacctaattc agaaatggat ggttggcatt taaagtttgc tttggcggtc aatatgttgc
    34321 taaacggtaa tagctttgca gagattaaac gcaacggtga aaaggtagaa gaaattcact
    34381 tattacctaa ctcaagtgta acggttactc aattagataa tggtacgttg tcttatcaga
    34441 ttggtgataa aaaaagacgt gtgaagtcta gcgatatttt gcacttcaaa tatttcaccc
    34501 aagatggttt aacaggatta ccaccacttt atgcgttacg tgatgaactg aaaattcaac
    34561 aagctggcaa ccgcacattg cacaatttct ttactcgtgg tgtcagtggg tcaggtattt
    34621 tgaaggttca taagtcagat ttagacggat cagcaaagaa cgcaattcgt gaaaagtttg
    34681 aagaagccaa cggttcaagt agtggagata atgctcttag aacgatcatt cttgatgaaa
    34741 caatggacta caaaacatta gaaataaaca ccgatgtttt gaagcttatc aattccaatg
    34801 attggaacac aaaacaaatt gccaaggcgt ttggtgtacc gattgagcgt ttaggcgttg
    34861 aaaatgaaca ttctagcacc gttcaaagta atctccagta tatccaaagt acgctgattc
    34921 attactttaa tgtgtttgtg agtgaattag atacgaaact tgaaaccaat atccgcttta
    34981 attccgatca gttactagag actgatcctg aaacaaaagt aaaaaatgta ttggatcagg
    35041 tcaaagggtc acttctcacg attaatgagg ggcggtcgaa aatggggcta ccccccatgg
    35101 atggtggcga tcgtttacta gcaagtttga actttacgta tttagatacg ttggagaaat
    35161 atcaattaaa agaacaggaa ggagttacac cagttgaata atgaagaaga aaaggaaaaa
    35221 cggctgacag aagaagctga gctaacagcc gattctccca aagtagagaa agaaaatgaa
    35281 aaacaaccaa cagacggcaa aactatttca ggctatgcgt tgaaattcgg gcaaccgtca
    35341 aaagatttag gcggctttgt agaagtgatt acacccgaag cattaaaaga ggtggattta
    35401 tcaaatgtgt tcttattata tggacacgat tattcaaagc cactagctag tgtcaaagca
    35461 ggtacgttga aattgaatgt cgatgataca ggattacatt ttgaagcaga gctaaccgac
    35521 acgacctatt caaatgatgt gtatgagaat atctcaaaag gggttgttga ttctatgtcc
    35581 tttgggttcg tgcttggatt agattcattc gacaaaaaag aagatggcac aattgaacga
    35641 tcaatagata aaatcaaagc acttaatgaa attagtgtcg tgaccgttcc cgcttatgat
    35701 tcatcaaatg tccaagtaaa taagcgttct tacgaatcgt ttatgagtaa caaccaagca
    35761 aaacaaacaa acaatagctt agaatccact tctaaagcac aaaaggagag taataacatg
    35821 gaaaaaactt taatcgataa cgaaaaaaca gaattgcgtg ggtatgaaga atatatccgt
    35881 tcacaaggcg aagtgcgtga tggagtcact actgtaaatg cagcggcagt tgttcccgaa
    35941 gaagtaatcg gtgaagtctt tgatttgaaa cgttcaaatt ataacttagc tcaatatgca
    36001 acagtaaaaa cagtatcaaa tggacaaggt aaatatccag tagcaactaa ccaacaagca
    36061 gtgttagcaa caaaagctga acttgctgaa attggtgata ttgacgctga aatgtttact
    36121 tcagttgatt ataaagtaga aactcgtgct ggtaagattg ccttatcaaa tgaggttgtg
    36181 gaagattcag cagtgaatat tgtacaagag gtcaaagatc agttagcgaa attggtagaa
    36241 aacactgaca ataagcatat catggattta ttaaaaacat tcactaagaa aacggctgct
    36301 acgttggacg atttgaaaca actatacaat gtggcattag acccagcatt aaataaaatg
    36361 gtaattctaa accaaagcgg atattaccac ctagatacat tgaaagattc agatggacgt
    36421 tacattttac aacccgatgt gacagcacct agtggtaaat cattattcgg tatgccagta
    36481 gtattgattg cagatacatt gtttgccaat cctaaagctg gaacattccc tatgattatg
    36541 ggggatattg cacaatctat ctttgttgct cgtagaaatc aagtaacgac tcaatgggaa
    36601 aaattcgatt actactcaca aggacttgca gtgatcgttc gcaacgatta taagaaaatt
    36661 gatcaaaatg cttcagtgta tattgagttt acgccagttg taacaccaaa agtatagaaa
    36721 aatattgggc ggcggtttat cccactgcct ttttttcatt aggagagata aaatggtaat
    36781 tttggatagc ataaaaaaaa gtatgcggat cgatcacact attgatgatg actttattca
    36841 acaattgatt gatacagcag gcgaatatat aaaaagtgct attgatagta gcgcaacgga
    36901 taaagatatg gataattatc agcaatttga tttggcggtg tcattactta ctcaacattg
    36961 gtatttgaac cgtcaagaag ccagcagcga acggatacca gtaacggtac aagcattagt
    37021 acaacaaatg agaggtgctt attatgccaa tcattaagaa tgtaaatgaa ttgactgaga
    37081 gagtcaagtt taaaaagaca aaacgggtaa aagatgaaga tgggcagatg gtggatagtg
    37141 aagaaactgt atttgaatgt tgggctaatg tgcgttcaca aatgctaaaa gacgtgcttg
    37201 ctagtgtagg taccattctt gaaggaacgt tgacgtttat tattcgatac gatcaagatt
    37261 atgaactaac aaacgatatg aaagtagctt ggaaaaacaa gagttataaa attatttcaa
    37321 tcaatgaagg aacggcgttt aaagattata caacgatcat agctaaacag atttcttaga
    37381 atgattacac ttgtaaacgt tgtgggtatt tagtataatt aaggtagtaa atgaagggtt
    37441 agctgcctga attttgcaag acttagctag tcgaaatcta ttgttggact gaaaattgta
    37501 ggtgtggttg caaacatgct ggactaagaa agatgataga tgatgaacgt tcaattttaa
    37561 tacaggcatg ctaggactga ttggatggtt ttggcaagct gattgtgtag aaatcaaaga
    37621 acacgtctgt tgtggacgtg ttttttttgt tataattcaa aagaggtgat ttaatggaca
    37681 aagcagagtt tacaaatttt tattggaagt attatctaca tttagaaaat gagtttatta
    37741 ataccacaga ttttgtgatg ctcgatgaga ataatttcaa aacattctct attgaatatc
    37801 aaaaactact actagcgatt ggttcggagt gtgagattat tttcaaagaa ctttgcggat
    37861 ttgatagcag tagcaataaa agcataactg attttaaagc tgttattaaa tctagcgaat
    37921 taatgtgttt ggataatggt gttagggtta tgaattcttt aacattgact tcactaaaac
    37981 cttttggaga tgggtggcct gaagatacac cagattggtg gaaaatatat aacagagtaa
    38041 aacatggtcg ttcttcaaat tataaggaag caaatttgga aaatgtgttg tatgctttag
    38101 caagtctata tttattagag gaatatttgc ataagaaagt aatactagaa ggtgaagtag
    38161 actttataag tccagagtca aatctattta cgttatcttg ggaaagaaaa cattcgtcga
    38221 tgcaaaaagt gacgtttgaa aaagtggatt caaattatgt agctccactc attgatttta
    38281 aagaacaaga agaaatttaa aaacccccac ggcttattga agtttttgta tgccatattg
    38341 tatgccattt ggtttaaatc gcttgaaaaa tagtgataga taactctaat tcatttagtc
    38401 ataaacgctg ttttgataac ctttatagtt ctttgatatg ggtttctcat tcatggcgga
    38461 agaagaagaa tagaacaaaa gaatgaatta ataagatata aaaaaaggga ttgtgacaaa
    38521 cgtcttgtca caatcccttt ttccaaataa acgatgttca atcagctgcc cctcggcatt
    38581 cgctcgaaaa acggcgaaat atgaagaact attttgccat ttgtttctct aataatcagg
    38641 tcaaaacgtt gacttcacag tctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc
    38701 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc
    38761 ggagctgaac ggcaggctca cgacctcttt cttaaacggt gttcaaaagc ttgcatctgc
    38821 ggtaataagt tcaaccaaac gaaaattatg aggagctatt tttgtttggc tgttcttatt
    38881 acttgatgca ggacagcttt ttcacaacct cttttcgttt agcactaaat ttccttaaaa
    38941 agtagaaaag ctttcgaagc ctcgtttgtt tcttttactg tataataaaa gaaaagagta
    39001 acaatcgcta aaagatataa acaagagaaa ggaaatacaa gatgattggt ctgatttatt
    39061 ttgtggtgat cgttctggcg aacacagttg gcgctatttc cgggatgggt ggaggcgtgt
    39121 tgatcaaacc gattcttgat cttatcggcg cccattctgt tgcagggatt tctttttact
    39181 caacagttgc ggtattcacg atgtccattg tttcgactgt gcgacaagta tcaagcggga
    39241 aatcactgaa ttggcagatt gtcggttggg tatctagtgg agctgttgtt ggaggagtag
    39301 tcggcaacat tgtattcgaa gtttttttac agttatttga aaacgagaag catgtgcagc
    39361 tgatccaaat ttttttgacg gtattgacct tggtttttgc gttcttctac accaagcatc
    39421 atcagccaaa atttcatttg acgtcatgga cttggtacct tatatgtggg gggggttctg
    39481 ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc tttgttgatg
    39541 ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac gattttcttt
    39601 tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg ctttgattta
    39661 tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg agcaagattt
    39721 agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt cctcgtggtt
    39781 cttttggtca attgttacaa tgcctttgtc atcttacaaa cattaggaga atctagacta
    39841 acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaagagta tactatgata
    39901 aaaaaattca ttttaaaaaa tcagaaattg gctggtgatt tggatgttac gggaaatgcg
    39961 tttagaagat gcagaagcac tacgtgaaat caatgcggat caattagggt atgatatacc
    40021 attaaccgtt acggtgagac aaatgaagaa actgctgaag gagccagaga aaaatttttt
    40081 cttggtttat gaagatccta cttctaaaaa agcagctggc tatgtacaag cggagtccta
    40141 tgaatcgatt tttagcgatc caatgttcaa tattatggca cttgctgtgt ctaaatatgc
    40201 tgaaaaaaga ggcatcggaa aggctttgat gactggcgta gaagaagaag cgaaaaaacg
    40261 aggaataacg acaataaggc ttaattctgc agaatacaga gtagaagcac accaatttta
    40321 tgaacacatc ggttatcatt ccgataaaat gcaaaaaaga tttttgaaaa tcctgtaaat
    40381 ctttatcaaa aaaatacccg atcaccaagc cgatacttga tgatcaggta tttttgtttt
    40441 tttgactaag aaagtaattg catcaagaaa ccagagatga tcaaaacgat ggcaccacca
    40501 agtcgattgc ccatttgagc aaaagcaata agttccatcc gattagctgc agataagacg
    40561 gctacatttc ctgttccgcc catcgagttg ttgcataagc cagcagtaat agttgattca
    40621 attggatata aaccaaacaa acgaccgaca aatcctgaaa caactgaaat cgcgataaca
    40681 cttgtcagac ataagacaac aaattgccat gtcaaagcag aagctaatac attcagattc
    40741 agcaacgcaa taccgatacc ggcaagaaca gctgctgtca agtttttgac aatcaagtta
    40801 ttgaacatga tagctgcatc ttcataatac tctggtaata ggttggcgat tttacagatg
    40861 acaacgataa taatcataaa tgcgtaagca tggactttag gaacaaaata gttaccaatt
    40921 tgtcctaaga tgaagaaagt caatgatacc atcaaaccaa cacctaactg tggaatggaa
    40981 ggtttcacag ttcgaggttt tccatcgcca agatcgcctt tttccattaa tttaccattg
    41041 ccgttgaatt ttggcattga ttgtccagca cgagcaacta atgctgcacc gatgatagct
    41101 aagacatttg tcattgctgt cgcaggaatc aggcgggaaa tgatagaaga agcatccgta
    41161 cctaaaacat ttgcataaat cgttgataaa ggaacggctc cggctccaac accaccagcc
    41221 atcgcaggta atgagatgta taaaacggaa tttgcaaaac catttccgat aagcatgccc
    41281 acgagtccta cggcaaaaaa ggagacgacc attgaaataa gtgcaacggg aataaaacga
    41341 acagaagctt tcatcaatag attacggttc ataccaagga tgctaccagt aatcaaggca
    41401 gcaatataaa agtcccaaaa gcccatattg ctgacaaagt tttctgtgga tttcactaca
    41461 tattcaggta agatcccaaa tgtagcaatc gcagcagaag cgaagatcgc aaaaacggaa
    41521 ccaccaccca aataactttt gacgataggg atttttgtcc cgataaaatg aaataaatta
    41581 ccgaaaataa ccaaaactgc gattggtcca atcatattcg tcggtaattt ccctaaagcc
    41641 attgcaatga tcaggacaag gaccatgatc aaataaagtg gcaaacttac gccggtgatt
    41701 tttgtggacc aaaaaccttc ttttttcttt tcctcaactg cagttgcagt tgttgttttt
    41761 tgtgccattt ttacagatcc tttcttattc tgtatcatat gaaaaaagcc aaagaaaagc
    41821 gacaaaagag caggagttgg ctggccgctg ttttgccgca gtcgcttttt tctttttaga
    41881 cgtcatacaa agtttctgta tgttctttta ctataacgat cttaatcttc taaagattta
    41941 tattcaggaa cccatttagc agattcaact gcttctttga tatccgtgat ttcttcacga
    42001 ttcaagtttt gtgcgacaac tgattttgcg acagtttcag caatcgtttg agaaaattca
    42061 gtgatttttg ctactggagg caagatagct gctcctggtt ttgttacatc aacgattccg
    42121 cctaatgctc gactagcttg agaaatgatt tcagcattta ctcgtgtagc tgttgaagca
    42181 atcaaaccaa gtcctaaacc tgggtacatc aaagcattat ttgcttgacc gatttggtaa
    42241 gttacaccat tatattcaac atcagcagca gggatacctg ttccaactaa tgcttttccg
    42301 tccgtccatt ctattaaatc ttttgctttt gcttctgcca attttgttgg attagacaaa
    42361 ggcatgatga tcggacgtgg tgtatgagca gccatttctt tcacgatttc ttcagtgaat
    42421 gctccaggtt gggtagaagt accaatcatg attgtcggat ggatcgcttt tacaactgct
    42481 tctaagtttg tcaattcatc agcattagtg aaatctgagc gtttgcgagc gaatgggatt
    42541 tgaccaggag tcaatccttc tgtatcttca aacaataaac cttgtttatc tacttgatag
    42601 aaatgttggc gtgcttcttc ttcaggtaca ccttgacgga tcatctcatc taaaaggatg
    42661 tttgcgatac ccacaccagc agtaccagca ccaaatgtca aaattatttg atctttcaat
    42721 tgttcaccgg atatattcaa tccgcctaat acacctgcta ggacaacaat tcctgtacct
    42781 tggatatcgt cgttgaaagt agtgatttga tcttcgtatt tttctaagat cgctgcagca
    42841 tttcctcgtc caaaatcttc ccaatgcaat aacaattctg ggaataattc ggtagctgat
    42901 tgaacgaatt gatcgatgaa ttcatagtaa gtttcgcctt ctacacgagc atgacgattg
    42961 cctaagtata agggattatt caataattct tcattattag taccagcgtc gatcgataca
    43021 ggcaaaactt gtgctggatt aataccagca gcagcagtat agaccatcaa tttgccgata
    43081 gcgatgtcaa caccgttcac accccagtcg cccataccta agattccttc tgcatcagtc
    43141 acaacaatca aacgaatgtc tcgtccatct gccgcgttct tcaaacttgc tttgatgttt
    43201 tctggttcgt cgattgaaag aaatgcagca tcttgcggtt tcaagaaaat ttcgttgtat
    43261 tgttcgattg aatccgcaac gacaggatca taaacgacag gcatgaattc aactaaatgt
    43321 tcgcccatca atttataaaa caatgtccga ttcgtattga atagattcat taagaagata
    43381 cgtttttcca aatctgtttg tttgcttaag tattgaccat aagcttgaac agattgttgt
    43441 tccagtgttt ggacagtact tggaagcata ccagtgattc catattttgc ccgttcttct
    43501 tttgtaaaag ctgttccttt atttagaaaa ggattattta ataagtttaa ccctgtaagc
    43561 atgaatgatt cctcctaagt tttaatacaa acgaagtata gaagtcagat gatgatatag
    43621 tcaaaaagag caggtatgat aaattttatt tatatgtaat atagccaaaa gaaatcaaaa
    43681 tagcagtatt tgttcttttt atatcgacaa tccttgcttg aatactatgc gtatctctta
    43741 tatgctagag aaagaaaagt agaagaataa gggatagatg gaatggataa ataggatttc
    43801 tgaggaaaaa agcacatgat agcgttgata ggagtcacct atgtcaggtg ataaaaatac
    43861 ataagtagaa aattaagagg aggaagagaa aatgaaagaa gttcaattga ttagaaaaag
    43921 cgaactatca gaaggcggtt gcaatgcatg tggcgtagta gaagcgacaa gctatacatt
    43981 aaaattagat tccaatcaag caataatctc tgaactgaca gtaggcggtc ttgtggacag
    44041 tctggcgctt gctgaaggtt ttactggaga agatatttat gagatgtttt cagaagtccg
    44101 tcagttaaaa aaaggagaaa aatgcataga ggttcatcat gagagcccga atgtgaggtt
    44161 caaacgtggt gataatgaga tgatcttcaa gaatcatgtg tctgatcaca ctgaactata
    44221 tgaaatcgtg aatcaaatac tgtctgggat ttttgaatta ggaccttatg agttcaagga
    44281 agaaaacggt aatccaaaat taaatgaaga atggcaagaa acgattgaaa cacaaagaaa
    44341 caatccacac ttatttcaat agtcttccac agtatttcgt aaaaatgttg atgaaaaaga
    44401 aacaccgtga atcagaggtc gtgaaaaacc tgccctgcat caagtaataa gaacagccaa
    44461 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagtttt
    44521 tgaacaccgt ttaaggaaga gactgtgaag tcaacgttct gacctgagta ttagagagac
    44581 aaatggcaaa atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg
    44641 attgaacacc gttttatcga gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa
    44701 aattttgtga aaatggcttt ccacattttt gcataaattt gacttaatac cgagaaaatg
    44761 gcttttgaac accgtttatt caaaaaaagg gactttgaca ctacttttgt caaagtccca
    44821 tcggtttttt atctattttt attacaatca tttcttccag agtagtgaca tctggaatgg
    44881 ctgaaatgta aacagattat ttgcctgtat caatgtcgaa gagtaatata aaaagtgtaa
    44941 acgctatcat attgttcatc attcaaattt tatagagaga aggcattgag atggaaaaaa
    45001 catatcaagc aggtacaaat gaaggaatag tagattttat caatatggaa gatttagaaa
    45061 tcgcggcttc acaagtgatt ccagctggtg gttatggtta catcagcagt ggtgctggcg
    45121 acttatttac ttatcaagaa aatgaacggg cattcaatca tcgtttgatt attcctcatg
    45181 tattaagaga tgtagaatta cccgatacaa ctacacattt tgacgaagaa atgctgactg
    45241 caccaatcat catggcacca gttgctgctc atggattagc tcatgtgaag gcggagaaag
    45301 cttctgcaaa aggtgtagca gattttggga cgatctatac agcaagttct tacgcttctt
    45361 gtactttaga agagataaga gaagcaggcg gggagaaagc accgcaatgg tttcaatttt
    45421 atatgagcaa agataatgga atcaatttgg atattcttga agtggccaag cgtaatggag
    45481 caaaagcaat cgtattgaca gcggatgcaa cagttggcgg aaaccgtgaa acagaccgca
    45541 gaaacgggtt taccttcccg ctgccaatgc cgatcgttca ggcttatcaa tcaggagtag
    45601 gccaaacgat ggatgctgta tataaatctt ccaaacaaaa acttagtcca aaagatgtag
    45661 aatttatcgc tgctcattct gatttgccag tctacgtgaa aggtgttcaa tcagaagaag
    45721 atgtctatcg atcattagaa tctggtgctg gtggaatttg ggtatctaac catggtggcc
    45781 gtcaattaga tggggggcct gctgcgtttg attcgttgca atatgtggca gaagcagtag
    45841 ataaacgtgt accaatcgtg tttgacagcg gtgtacgacg tggacaacat gttttcaaag
    45901 caatcgcatc aggtgccgat cttgttgcta ttggcagacc cgttatttat ggattgtctt
    45961 taggaggaag tacaggagtc catcaagtgt ttgatttctt taaaacagaa ttggaaatgg
    46021 tcatgcaatt agctggaaca caaacagtag aagatatcaa aaaaataaaa ttgcgtgaga
    46081 atcgttttat ttaagaaata aatgacgaaa aaaataggag tgcggcagat gatgccgtac
    46141 tcctattttt atagttcaag gatgtcacta atagaataat ttgaaacgaa aaaataaaat
    46201 tgaaatattt tttcatactt aataaaaaag gaagaatatt catgtaaacg attttattgt
    46261 ttttttctct tttatacaaa aagggtcata atttcaagta agcggatgaa ttcatgagga
    46321 ttttttgcat actgtctcta ctatacttag gtcactaaga aaaaggagtg atttattatg
    46381 aaaaagaaca tgattgggac attattactt tcaacacttc ttctaggcgg cttggcaaca
    46441 ccagcttttg ctgagggtca agcgacttca aaaggagata ttacctttac tgaaccaaca
    46501 aataccgttg agcctcttaa ccctactgat ccttcaaaac cagtagagcc agcagatccg
    46561 gaaaatccag caactggcca gacaggttct ttaacattgg atgtagttcc tgaattgcca
    46621 tttggtacac acgagattga aagcggaaca aaaacgtatc aagtagacgc ttcaaaaaac
    46681 gatacacctt atctccaagt atcagatcgt cgaggtgtag gtgcagatgg ccaagcacaa
    46741 gggtggaacg tgacagtttc ggtttcagat tttgtaaacg gttcacaagt tttgcaaggc
    46801 gctgaattag attttggaac atccactgtt aaaagtacgt cagataacga atccactgga
    46861 ccaactagtc aaactgtaac tgggttgagt aaagcaagtg cagcgacacc aatatttaca
    46921 gcagcaaaag atcaaggtct gggaacatgg ctttctgttt atgatccagc aaatatcaca
    46981 ctgaaagtgc cgaaagcagc agctggaaca ttcacggctg atttgacttg gaacctcgtg
    47041 gctggcccag tagcatagag tatgaaataa attcaacaaa ctgcggtgaa aatactatag
    47101 gtgaaggatt ttttaccgca gttgttgttg aaaggatgtg gtggacaatg aatcaaagta
    47161 aacgactgtt tgtttcgttt ttttctattt taagtctatt tttcattttt ccgtcaatca
    47221 gccaagcaaa ggattcagca ggagactttg gaatcaaacc ggtatttcca gagaaccaaa
    47281 tagataaagc aatcggttat tttgatttat tagtggcacc ggaacaaaat cagacactag
    47341 aagtaattat cagcaattct tcagatgaag aacgaacatt tgaggtctct gtcaatccgg
    47401 cggtaacgag tgatggtgga acgatcgatt atagtcaaaa aaacccaacg ttagatgaga
    47461 cattgccgtt tgatgtccgt gacgttttgc tcattgcgaa aaaggaaatc aatgtatcag
    47521 cacatgcaga aacaactata cctatagaag tgaaaatccc agcaaaaagc tttaagggac
    47581 gtgtattagc gggtattcat gtttcaccta aagaagaagc tgaaacagaa aatgctacag
    47641 aaggagcaca aatcaaaaat cgtattgctt ataatctcgc agtcgtttta caagaaagtc
    47701 aagagacgat agaaccggat ctgaaacttt tatcaggaga tttggacgag gtcaacgcaa
    47761 agccaactgt tcagcttcgt ttccaaaatc ctcagcctag gattatcagc aatttgatat
    47821 ttacgagcaa gatcttttat gaaaatcagc tttatattga aaatacgtct aatgaatttt
    47881 tagttgcacc aaattcgaat tttcacttga atcttgattt agctggtgat aaagcaaaag
    47941 ccggcgatta ccgcgcagaa atcatagcaa aatcaggcga cagtaatgaa tggcgattta
    48001 cacaaaactt tacgatcaaa aaagaaaaag cacaaaaagt aaatgaaaat tccgtttttg
    48061 cagtacaaga acaatctttc ccatggcttt atgcaggatt ggttgctgca gttctagtgt
    48121 tatggttgat tttgctgttt ttgtgggtcc acaagaaaaa gaaaaaagga gaaacaacat
    48181 gatgaggtga tttcgttgaa aacatcgatc ttattgggag ggattattgc aagtttgctg
    48241 ttcttcagct ggcaagagcc agtccaagca gataccaaaa gttcaggtac gattacattt
    48301 tatgatctaa gtccagttcc gcatccaagt ggttcagtca atggcgcatc aaaacagcag
    48361 acacagctac cgatttataa agaatctaat caacatggcg tattcccgga gacgaatgaa
    48421 gaaaagatcc gttttcttta tttgatcatt ggtatagaat tatggcttct tgcgtggttg
    48481 ttgttatgga gaagaaagga aaaggaggat gaacatgttg ctacaaaata aattttcatt
    48541 ttttcgctcc ttctttttta cttcgctttt tgttttaagt atatttatct tttcaccttc
    48601 ggcagaagct gttggagcaa cgcctcctgc catttcttat gatccataca cgtatgaaaa
    48661 tatcgtacct ccaaatggtg gacgtagtgc agtcaatggc gggaagcttg tccaactaac
    48721 tggggctaac caattgccta aattgactgc tacttatttg aacgatggtt caaatgatcc
    48781 ttggaaaggt ggcacggtcc gttttcattg ggaaagccat gtgaaactaa caaatgtact
    48841 tgatttggtg aatatcactg cacaaggatt tgcatttgtg gcgatcatca atttgccgga
    48901 acatgtatca agcagtgatg tattaaaagc aattgactgg aacaacgcct acttgtatat
    48961 tgccggtgcc cccatcaagg ttactagcgg tagtttatta tcagcaggaa gccatacctt
    49021 gcgttttggg atggggtcgt gggatacaaa tactctattg gcagatatta ttgctattat
    49081 tacgcgaggc gaattcaacg tggacgatat cccgcttcaa tttgatgtta cagctaatgt
    49141 cgcgaagatg acagaaaacg gagataaata tgatacgagt ccaaacaaaa ttttgaccaa
    49201 gggtaaatta caaccttcta tgtctaaaaa ggccgttttt tctgttgatt tttatgattc
    49261 gaataatatt atccaagatc gtatgttcaa ttcgatttta gctccagctg caggtagatt
    49321 ttatcctaaa ttaactggcg gaaacttgaa tccaggagca tatgcatttt ctgcaagtgc
    49381 agagataaat acgtggaata gttacatctc aacaagtgat tcaacaggaa aatataatac
    49441 atttgctgat accacagaat acgtggatgg aaataaccag aaccttccag gctctaccaa
    49501 tagtcggaca ttgacgatgg ggctgactga aggacgtttt gatcagttgc catttagtcg
    49561 ctttgatcgc gtagtcaact attttacagg aaaaaacgtt acacaaggag caatactgac
    49621 tcatactcca gcaagcacaa gtggattgaa tcagacagcc aatatcgtgt acagtgggaa
    49681 agatgcgcaa ggcggattgc tttctcctgt tgctttaaag gtattggagc agtacaagct
    49741 agacggaacg gttttaccaa caaacttaac agccgatagt cagtggggaa agctcctttc
    49801 ttctgcatcg taccaagtca cggccaattg gtcagcaact gaactaaaaa agggttcgat
    49861 ccattacagg ctttattcga aaggaaccca aaaattagcg acaggatgga cagatcaact
    49921 tttccaaacg atccaatcaa ataacggggg tgtaaataca gctacttcta cactacctgc
    49981 attggcttca gggcaatatt attttgatta tcgcttggtg gacgatgtac ttactaacat
    50041 gtatccgtct tttgcgtaca aatggcagtc tgaacaagct ggaatcacca ctcttcctca
    50101 aataacagtg gctaattttc catctgtgag tgcagatagt cagctaaaaa accttagcag
    50161 acaacctgag acaggagatc ccctgtctgc tctatcagga gatacgattc aagaaaatgt
    50221 gacattcacg ttaacaaaat ctggggatgc gataaccgac aaaaaaatca cgatctcact
    50281 tcctgaaaat actacttata tcaatggttc cttaaaatta aatggtacaa ctatcgcaga
    50341 taccggcatt caacaaggag tttctgtccc agcagatcta ttgagcaaaa taggagatac
    50401 cattcatctt acttacaact atcaattaaa tactgtagat acttctgtac aatccgtcag
    50461 catactgaca aaggcagctg tactttccag caatattaca ttagcagatg gtgctaagct
    50521 tcctaaccca gttgtccaaa catctgctaa aacaattctt gtccccaaac aagaattaac
    50581 attagttaat gtaccagatg attttacatt tggtaacgat ctccccaaac cgttgaagac
    50641 aagttattat gaagcaaaag gcgatttttc ttttgatgtg cgggatactc ggcttccaag
    50701 cacgtctcct tggcaactga cagggacatt gacttcttta tttaagaatg atcaaggtca
    50761 agaattaagc ggaacaaaat tatacttcaa tcattctgga tcaaaacaat tgattcagca
    50821 aggtcaaaac acgttgattt atgagagtga tggaacagct aaaggagagg ttctcgttga
    50881 ctttcccgat accgatggtt tactcttaga ggtaaatagc agtacgaatg ctcaacctgg
    50941 cgcaacgtat caaggcatgg tcacatggga actgacagct gggccaacca gctaagtgat
    51001 tagaatttgt atggatctgt gataactgtc acagatcctc tttttcaagg atgagaagga
    51061 aacttctgct gttaaaatca gaaatgcttg gaggagtgga cttggaactt ttcagtttgc
    51121 ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact tttattagag gaaaaaggag
    51181 agatttccgt taataacgta cttacccgct tatcagttga tcgggctacc ttgaaagaag
    51241 atctgtttta tttacaagct aacttggaaa atatcaaaca attggaaatt gttatccaag
    51301 aagggaatat tttattaatc agacatggaa atatagcagc agtcgatgtt tattatcatt
    51361 gctacttgcg ctattctaca aaatatcaga tacttcttta tctgttggaa aatggctcat
    51421 ttgatcggca gaaacttgtc atggaactta atatcagttc ggctactttg tctcgcagag
    51481 tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa aataaaaagc gggcggctga
    51541 ccggtccaga aagtcaaatt tgttattttt atttccaatt attttggttt ggacgtcctt
    51601 atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa cactttttta gatctgctgc
    51661 aaaaagagtt tagttttact attacagaag aaggacgatt gaaatttgaa atatggcttt
    51721 ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa accagattat agcaatcttc
    51781 ctgttggatt gtttcaaacg acacaatttt tattggacct ccatacattt ttgacccgtt
    51841 acttctttca atatgccttt ccttggaatc aaaatgaaac gttggttttt tatcttttca
    51901 tgatcagtaa ctttacatta gatacacata actcgcatgt ccaggagttt ttgataagcg
    51961 aaaaatacaa agacgacgta gtagctcaat tgaatcgaac gtttaaacaa tcgatagaag
    52021 aatatttctc tttttatgtt tttccagaag agatttcgca aaagattgcg ttgacagtga
    52081 tgcaaaatca cgctcatttt gcctatttta gaggatggat gaatattttt ggccaagaag
    52141 gattaaaaca gcggttaact gaagtagaag ataaacaatt cctggtttta tgtgaaagat
    52201 tggtcagaca aacatttgct attgttgatt ttactgaagt agacgaggaa aatgcccgct
    52261 ttgaattatt tgctcgctat gcatctattt tgcggacgct gatcaagcag ttagaattac
    52321 gtctgcaaat tgcttatgat cttccttatg aaaggctaat ggcggatatc gtgaaagaaa
    52381 tcatccagga acaattgctg aatacgatca aatatgattt agtcgaatac gagaaagaaa
    52441 agcagtatga tatcattttg actagccaat tgaaagagta cccgtcacag aagtccgcta
    52501 aagtttttgt attcactagc aacgaatata attatgattt cccctatttg aaccaatttt
    52561 taaaagaatg ttatttagaa aaattgaatt taaggatgaa taaaacatga aaatcgtgat
    52621 cattggtgct tcccatgcag gcatcacagc tgccctaaat ctaagaaaac ttcaaccgaa
    52681 agcagaagtt ttactgatcg atgaagatca taaagatgga ctaggctatg tttcaaatgg
    52741 aatcaacctt tatttaaaag gaaagatacg tagtttagct gaagttgccc ataacatgcg
    52801 gactcttcaa gaaagtgggg caaaactgat caccgaatgg agagtaactg aacttgatcc
    52861 agacaaacat cagctcatcc tcgcatccaa agaaggaaaa aacgagacca taacctatga
    52921 taaattaatc gtagcaacag gttcctctcc tgtcaccttg tataaacaaa ttgaagctga
    52981 aaatgtctac acctataaaa atctagtgca atctaagcaa gtacttgccg ctttaaaaga
    53041 agcaaaagaa gtagtgatct tcggtgctgg ttatattgga ttggaattag cagatgcttt
    53101 gagaaataaa ggatacatta ttcatctagt tgattatatg ccgaatgttt tatcaagata
    53161 tttcgataag gatatgatca acagttttca aaatcagctg caaactaagc aaatcaattt
    53221 ttatccaaat gaattcttga tcgattggaa aaaatcagaa gagaaagtag tatccgtaca
    53281 acttctatct caagcaataa aggcagatat ggtcattttt tctgcccaaa ctcgtcctaa
    53341 tacgacattg ttaaaagaaa aagtggcttt gtatgaggat gagactgtga tagtcaatga
    53401 atatctgcag acaagtgatc cggacatcta tgcgattgga gatatcgtcc cagtcagttt
    53461 tgacaaaaat aaacggcatc tctttttacc tttagttact cgagctgtac atatggcaag
    53521 agcagtagcg ctaactttga gtggtcagcc aaccgcttac gacctcagac aaaagataac
    53581 agcagcagtc attatagact actttttagg aactgtaggg ctgacagaag atgaagcgcc
    53641 attcttggaa caaaataccg gttcgtgttc aggagaattt gatctatttc cacaatacga
    53701 tgaagaaaac aaaacagtaa atgcaaagct gatctaccat ccggatacat tagaaataat
    53761 tggcggacag ctgataagcc aagaattctt attatcagat ttgaacctgc ttgcggatat
    53821 cgtgaaacac aaaactacga ttccacagct agcagttgag gattttggat ttctgtctga
    53881 acacacctct cgttttcact acctcaatga gttagctttt aaagtactag ctaagagagc
    53941 aaatagcaat agagtagata aaagactgtg agaaatatca cggtctttta ttgtgtgggg
    54001 atataataaa tattaatcaa tcgagaataa aaaattcata taatgaaata gcggaagaat
    54061 tttttgagca taatattcgc caaaaattta tcaagtgata aaattgttgt ataaaatgat
    54121 tatttcagga ggaggagtta aatgttttta gcattaaatg aaattatgca ttcaaagtta
    54181 cgttatgcgt tagttgcagg ggtgatgttt ttgatcgcct acctggtatt ttttttgact
    54241 gggttagctt atggcttagc tcaagataat cgaacagcag tcgacaagtg ggaggcagat
    54301 tcgattgtgc tttcgaaaga tgcaaattca aatctaggga tgtccatgat tactaagaag
    54361 atagcagaag aagtcgaagg tgggaaagtg gcgtatcttg cccagacacc tggagtcgtg
    54421 actagtaaag acagtacaga agaaggaaaa atcaatgtta gcttcttcgg tatcgataaa
    54481 aatcaattca tcatgcccaa tcttgttgaa ggaaaagctt ttgataacga tgatgaggca
    54541 gtaggcgata ttagtttgaa agaagaatat ggtctagccg taggagatac agttaagtta
    54601 tctggtagtg acaagacgtt taaactaact ggctttacgg atcatgccaa attcaatgtc
    54661 tcaccagttc tctatacaac catcaatgct taccaagaga tccgcttcga aaaagaagat
    54721 acaagtgaaa atgcacggat taacgcaata gttgtcagag gtaaaatcag taatttacct
    54781 gaagatttag aacagatcaa aatttctaaa ttcatcaatg aattacctgg atataatgcg
    54841 caagtattga cttttggatt tatgatcgga tttttgatcg ttattgcggc tattgtgatc
    54901 gggattttca tttatgtact gacgatgcaa aagattaata tctttggtgt aatgaaagca
    54961 caagggatca caggaggatt tattgcgcgt tctgtagtgg cacaaacatt cattttatca
    55021 tttgttggta ttcttcttgg tctattagga acagtgggaa catcccttgt tctacctgat
    55081 gcggttccat tccaaagcaa ttggctgttt tttggcgtca ttagcttgtt gatgctggta
    55141 gtagctgtgt taggcgcact attttctgtt cggacaatcg taaaaatcga tccgttgaag
    55201 gcaattggat aagaaggagg aagagcatga aagcaataga atttaaagaa gtagaaaaaa
    55261 gtttccaaga tggcgaccaa gtagttaaag cattgaaaga aacaaccatg agtatcgata
    55321 aaggggaatt tgttgcaatc atcggtcctt ctggttcagg taaaagtact tttttgactt
    55381 tggcaggcgg attgcagacg cctagcagtg gctctgtatt tatcaatgat caagaattca
    55441 gtaatcaaaa agaaagtgaa cgcgttaaac tacgttttaa agaaattggc tttattttgc
    55501 aagcttccaa tctggttcct tttctgacag taaaaaagca gttattatta gtagataaga
    55561 tcaaacatga acatcgtcaa aaagaagcaa aagaattatt tgagcagctt ggcgtggata
    55621 agttgatcaa caagtatcca gaagaactat ctggtggaga acggcaaagg gtcgcaatcg
    55681 ctcgtgcatt gtacaatgat ccgtccatta ttctagcaga tgaaccaact gctagtttgg
    55741 attcagaaaa ggcacgagaa gtagtcgata tcttggcaaa agaatcaaaa gacaaaaata
    55801 aagcaacgat catggttact cacgatacac ggctgattga caaatgcgat agaatattta
    55861 tgatggaaga tggtgtattg aaagaaaaag aataaccaaa aagcaggtgg aaaaatggtc
    55921 catcgataca acaaagatac acagcaaaaa attttgaaag aagcgactga cttgtttatg
    55981 tccaagggat atctcggcac ttcaacaaga gagatcgcac aaaaagttgg gatcacccaa
    56041 cccaatctct atcattattt cggtgataaa gaaaaactat atacggcagt tttagaaaac
    56101 cacttaaaag atgttggaaa agctttgaga gaaatcgttc aaacaagcga aacgggattt
    56161 caagtaacct tgacaaaaat ggcgcagttt ttgattgaga cacatctggt cgatttgttt
    56221 ctgatgcttc acgatcttga aagcaatctt tcaaaggaaa caagagatca tcttttcttt
    56281 ctatggaaga aaaattatcg tgaacctttt gaagaaatct tttcagcaaa ccaatctgtg
    56341 ctaagaaatg ggatttcaaa agaaatcgca gcaaggcatt ttttcttagt gttagcacct
    56401 tacatcactc ggtcgaaaag acctgtggaa gaaccgctaa atgtggcgca attgatcgat
    56461 ttatatttga atggtgtatt aggagaagcg taaagctgtt atatagggag aaaaatagtt
    56521 aggtgaagaa gcaagatatg acgaagatcg ttatatcttg ttttttttat ataggtagtt
    56581 tttcataaat acaaatttgt tattctctca tgattatcag ggtatatttt tatttattat
    56641 gtcggtgttc caggaagtgt tttaatttaa taacgaagaa attaattgtt tgtagaatga
    56701 aggaaactat ttattgggta gatgaatcac cacgaatagt caaaaggaga tttcaggata
    56761 tgccaaaaat gaaaaaaatc attttagcag taatgttatt ttctacggtt atatctttta
    56821 cagccccttt tttgcgtact taagctgatt ttaagttttg acccttagtc tggatctacg
    56881 aggtgagagt caagaatgaa aaaaataatt ggagcttttg tatttgcaat cattatcatt
    56941 attatctgtt ttcaaataga tgtttttggt caaaaaaaga tagttggaca aacggtcagc
    57001 agtacgaaca gtacggttac aaagacaaag gaatcatttg atgatacaga acaagagaca
    57061 ttaatcaaaa tcggaaaaaa tgaccgtaca ctatatacag atgactcgct aactacccca
    57121 cttgcaacta taaacggcgg agagctggcg gagtttcttt ctgaaacaaa aaatgcttat
    57181 cagatcaaga caaatgatgg ctatactgga tatttagccc tcgaagatgg aaccaaagtc
    57241 acaaagaata tacaaacaaa accggaagag ctatctgatg cagtgattgt attagatcct
    57301 gggcatggcg gaaatgatac tggggcatta agcaatgatg aacaaacaga agaaaaagat
    57361 ataacattaa gtacagccat aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt
    57421 tatttgaccc atactacaga tgaattagta cagcttggag atatatgcga ctacagtgaa
    57481 gaaaaaaagg cagatgtctt tatcagttta cacgcagact ctacggaata tgcgaatgaa
    57541 gcaactggaa tcacgacata ttattactat ggacaagagg aaacattagc acagacaatt
    57601 gctgactcct tcactgatct gcctctggat tcaagaggaa tctccactgg gaactatcaa
    57661 gttcttcgag aaaatcttca accttctatt ttaatcgaga tgggttatat gaataatgat
    57721 agtgatttaa aggaattagt aacagacagt tatcagcagc agattgctgc aagtttgaca
    57781 caagcattga ccacttattt tcagtaataa ataagcccag ccaaacaaaa atagctcctc
    57841 atatttttcg tttggccggg cttattatcg cagatgcaag cttttgaaca ccgtttaagg
    57901 aagaggttgt gagcctgccg tttaactccg cgtcacaagg agcaatttca aaaaacagct
    57961 tctcatgttt gatgactatt gtgacttgtg accgaggagt tggcatgaga acaccgttta
    58021 ttcggaacaa gggactgtga catgactttt gtcacagttc cattattttt ttagcagctt
    58081 caaagctttt tctgtattgg caaaatgaag tttggcatat ttttcaagca taggcatgcc
    58141 gcctttctcc aatagttttg ccgctacttg gtcagatttc gttccagctc ctgatggaag
    58201 ggtgattcca gcttcagctg atgctttgtg taactcttcc aaaaagctgg cacgactgat
    58261 gatagaagca gcagcaacag ctaaatgata ttgttctcct ttagtaacga agtacagttt
    58321 ttcttgtaat tgattttttt ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa
    58381 ctgatcgatc aaaatcgctt ctggtttttc tggctcgatt ttttttagca gcaaatggat
    58441 cgcttgattg tgcaaagcga ctttcatccg aacggcattg tattttggtt ggatctcatt
    58501 gtatttttct ggagataaga caagcagttt ataggggatg gtttttttta agatgtcagc
    58561 aagctggatg atttggctat cttttaactc ttttgaatct ctaacaccaa gtttacgtaa
    58621 ttctgcaagc tgatcacgtt tcacataagc agcacatact gttagcggac caaaatagct
    58681 gccatttccg acctcatcac tacctaaaac ggataattga ctgatatttt ttggcaaaga
    58741 agaggctgtt tttgctggct tggcttgttt atcctgtgcc tccccccatt tatcagcttc
    58801 tattgctgca ttcgtacctt gaaacatcac ttttccggat gtataagcgg taatggtcgt
    58861 cccgtctttt tttgcagcaa agctagtata aggaatctgt ttatttaaca aataagagcg
    58921 atagtatgct ttcatttgac taagttggtc gtttgttgct ttgataatga cgttgctcat
    58981 aaaattctcc tcgtttttat acataaaact ataccgcaac tcacgacttt tcgttttcta
    59041 agagattgcg agacatagca tttttttaaa gtttatcata taatagttaa gtatgtgaaa
    59101 aatatttgtg gagaacggtg aaagaaccaa cttcatggta cactaaataa aactttcatc
    59161 aaataggagg aaatgattcg tatggcgcac gaaaaaacac gctacaaagc agtgattgcc
    59221 aaccaaacat atactattat cggacgggaa accaagcatc atatggatat cgtaacgaag
    59281 ctgatcaatg aacagttagc agaattgaaa caattatcac cacaaatgga taatgaacaa
    59341 gcagcaatct tgatggctgt caatgcgctt tcggatcaat tgaagaaaca agaaagaatc
    59401 ttggagctgg aagaagaaac agcagaatta aagaaaaaaa tgatcaaatt tacagagctt
    59461 gaaaatcgtg tgaaacgcat tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa
    59521 aatggtcaag cagatcatac cattaaaaat catgtggaag cacaacagat tttaaacgag
    59581 aaacgaaaag gacaaattaa acaaaaatct tctaattaag atccttttat caaaataaga
    59641 aaaatttaaa aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct gattttattt
    59701 ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca aatcatattc
    59761 gcgattggat atgtattgtc atttattgct gcccagcatt tttacaaacc attagcaagt
    59821 catttggaat tatacattcc atacccagct gtcaccccaa catcaaagtt agcatttttt
    59881 gatcaggtat ttgcttttca cctagatgaa gctttttatg caggaactgc ttttctgatt
    59941 attttgctaa ttggctggct gcttacccga tttgtggggg tttttgtcca tggattgacg
    60001 tatgtgccga tcttgagaca ggtagactgg attgcaggag gcatcctgtc actaatcatg
    60061 gcttacgtta ctatattttt gattttgcag ttacttgcct ttgttccatt agtgtggtcc
    60121 aaaatcaatt cggcgagcat agtttcgctc gttttatcgt tgagagaacg ccatttctta
    60181 ccaacaagat gtatgagctt tgggtaacga atattttgaa ttaaggaaaa tagtgctgga
    60241 aaatatttcg ttttttgtac agagtagttt atttaagaag gctgcgtagc gtatgtccgg
    60301 cacagctctt ttttcatagt gaaagataag gtgataaaat gaataaccga atccttgaaa
    60361 cattggaatt tgaaaaagtc aaacagatgg tcagacaatt tgtagtgact gcacaaggaa
    60421 aagaagaact ggcagaattg gtaccagtga gtgagaaaca aacgatcaca aactggcttc
    60481 aagaaactga ggatggattg aaagtgcaac gtctgcgagg cgggatccct atacctaaac
    60541 ttgaaaatat ccgtccccat atgaaacgga tcgagatcgg agcagacttg aatggagtgg
    60601 agcttgcaca agtttccaga gtattgtcta cgacaagcga attgaatcgt ttcattgatg
    60661 atctttctga cagtgaaatc gaatttgcac gtttgtatat gtgggcagat cagttagtca
    60721 cgatccctgt tttgagccgc cgtttaaagg aagcaatcga tgaagacgga cgcgtgactg
    60781 atgacgcttc tcctgagtta aagagcataa gacagaacat tcggcgaagt gaacaagctg
    60841 ttcgtgaaca gttagatggg atcgtccgtg ggaaaaatgc aaagtattta agtgatgcga
    60901 tcatcaccat gcgaaatgac cgctacgtta tcccagtcaa acaagaatac cgcggtgtat
    60961 ttggcggtgt agtccacgat caaagtgctt caggacagac tttatttatt gaaccaaaac
    61021 aagtggtgga tttgaataat cgtttgcgtc aataccaaat cgctgaacga aatgagattc
    61081 aacgtatttt aagtgaactt tctgctgaac ttgttcccca tcgtcaagaa atcatccaca
    61141 atgcttatgt gattggtaaa atggatctga tgaatgcaaa agcacgattt ggtaaagaag
    61201 taaaagccat cgtgccggga atcagtgaag acaatcatgt cgtcttgaaa caagcaagac
    61261 atccactgat cgatcaggaa aaagtagttt ctaatgacat cacaatcgga aaagactatc
    61321 aagccatcgt cattacagga cctaacacag gtgggaaaac gatcacgttg aagactttag
    61381 gtctgttaca actgatgggg caagcaggat taccgattcc tgcaggtgaa gaaagtcaaa
    61441 tcggtatttt tgaggaagta tttgcggata taggagacga acagtcgatt gagcaaagtc
    61501 tatcgacctt ttcttctcat atgacgaaca ctgtagatat tttatcaaaa gtcaatgaaa
    61561 aaagtctggt attatttgac gagctcggtg caggaacaga tccgcaagaa ggtgctgctc
    61621 tagcaatcgc aatcttagat gatcttggta aaaagtctgc ttatgtgatg gcaacaacgc
    61681 attatcctga attgaaagtt tatggataca atcgggcaaa tacgattaat gccagcatgg
    61741 agttcgatgt ggatacacta agtccaacgt atcgtttgct gattggcgtt cctggacgaa
    61801 gcaatgcgtt tgaaatctct agccgattag gtcttgatac agaagttatt gatgaagcga
    61861 agcagttgat gaatgatgag agccaagatt taaacgaaat gatcacagat ttagagaatc
    61921 gccggaaaat ggcagagacc gaatatctgg aaatgcgtca ttttgtttct gaggcacaag
    61981 aacttcacga tgatttgaaa gaagcttaca gctatttctt tgaagaacgt gaaaaagaaa
    62041 tggaaaaagc aaagaaaaaa gcaaacgaag tcgtgtcaga ggcggaagaa aaagcagaaa
    62101 aaatcattgc tgatattcgt aaaatgcaac agcagattgg gcaaggaaat gtgaaagagc
    62161 atcagttgat cgatgcaaag acccaattag caaatctaca tcaagaagaa actttgaaga
    62221 aaaataaagt attgaagaag gcgaaagaac aaaaaacgtt gaaaccggga gacgaagtgt
    62281 tggttacgac ttacggccaa agagggacgc ttttacgtaa aaacgggaat cagtggcaag
    62341 tagaaatcgg tatattaaaa atgaatgtgt ccgaagacga gctgacgcct gttgctcctc
    62401 aaaaagagcc gacacaacga gttatccatg ctgtacgatc tgaatctagt agtcacgtgc
    62461 cgaatcaatt ggaccttcga ggaaaacgat acgaagaagc gctaagtgag gtagatcaat
    62521 atttggattc tgccattcta gctggttatc ctcaagtgac tattgtccac ggaaaaggaa
    62581 caggcgcact tcgcaaggga attactgatt atcttaaaaa ccatcggagc gtcaaaagct
    62641 ttgagtttgc accggcaaac caaggcggaa atggtgcgac aattgttaaa tttaagtaaa
    62701 gcttactaaa ggtaagcaga tggttcgcta tccaagtata gaaatgctat actaatcata
    62761 gattttaaaa atggaggtct ttgatatgtc acaagtaatt acagataaga catttaatga
    62821 agaaacagat aaaggacttg tattgattga tttttgggca acttggtgtg gtccatgtcg
    62881 tatgcaagca ccgattttgg atcagttgga acaagaatat gatgaagaag aattcagaat
    62941 tgctaaaatg gatgtggatg aaaatccaga aactcctcaa caattcggta tcatgagcat
    63001 tccaacatta atgttgaaaa aagatggtca agtagtagaa aaagctgttg gtgttcattc
    63061 aaaagaacaa ctacgtcaaa tgatcgatca atatttataa aaacttacag caaaaataag
    63121 ctgagggtgg gactgaatcc agttccgtcc tcggtttttt tgcggttgag ttaaggttat
    63181 tcaaattaat attttttgag tataattacg ttgtcaggct ggattgtttt tttgacagac
    63241 atgctataat aactttaatt gaatatgaag gggagtacca gatgaacgaa cgaatcaaaa
    63301 acaaattagc tttacttcct gatcagcccg gctgttactt gatgaaagat aaaaatggaa
    63361 cgatcattta tgtcggcaaa gccaaaatat taaaaaatcg tgtccgctca tattttactg
    63421 gcagccataa tacgaaaacg gaacggcttg ttagtgaaat cgaagacttt gaatacatcg
    63481 ttacagaatc gaatatcgaa gcattgctct tagagatcaa tctgataaag aaaaatgatc
    63541 ctaaatacaa cattatgtta aaggatgata aaacctatcc tttccttaag ataacgaatg
    63601 aaaaatatcc ccgtttggtg attactcgta aagtactaaa agacaaagca ctgtattttg
    63661 gaccatatcc cgatgtcggt gcggcgaatg agacaaagaa gatcctcgat cgattatttc
    63721 ctctgagaaa atgcaaacct agtcagacga aggagccttg tctttactat catttgggac
    63781 aatgtctgtg tccttattat ttcgacgtag atccgaaagt ctatacgaaa atcgttgatg
    63841 aagtgaaaaa gtttttaaac ggaggacacg aaaaaatcga gtcagagatc catcagaaaa
    63901 tggaaaaagc tgctgaaaac atggagtttg aaaaagctgc agaataccga gaccagatcc
    63961 gtgccattga gacaatcatg actcgccaaa aaatgacaaa tactgattta ctggatcgag
    64021 atgtatttgg ttatgcgatt gacaaaggat ggatgtgtgt ccaagtgttt ttcgtaagac
    64081 aaggaaagct aattgaacga gatgtttcaa tgtttccttt ctataacgaa gcagaagaag
    64141 atttcttgac ttacctcggt cagttttatc aggaaaatga gcatttcatt cctaaagaag
    64201 tcttgattcc tgatacgatc gacaagccca gtgtagaagc tttgttagct acgaaagtgc
    64261 tgcaaccaaa acgaggagaa aagaaaaaat tagttgaatt ggcgaataag aatgcctcag
    64321 ttgctttgaa tgaacggttt gatctgattg ccagaaaaca ggaacggacg atcggagcag
    64381 ttgaacgttt aggaaacgcg atgaatatcc cgaccccaat acgtatcgag tcttttgata
    64441 actctaatat catggggacg aatcctgtgg ctgcgatggt cgtattcatt gatggaaaac
    64501 cggcaagaaa agaatatcgg aaatataaaa taaaaacagt caacggaccg gatgattatg
    64561 catcaatgag agaagtcatc tatcgcagat attcacgagt gattcgcgaa gggttgcctt
    64621 tacctgattt gatcttgatc gatggaggaa aaggacaagt agatgtggca aaagatgtct
    64681 tagaaaatca attaggaatc gatattccta ttgctggcat ggcaaaaaac gataaacata
    64741 aaacaaatga gttgctattc ggtatagatt tggctgtagt accgcttgag cgaaattcac
    64801 cagagttttt cttactccag cgaatccaag atgaagtcca tcgattcgca atcactttcc
    64861 atcggcagct acgcagtaaa aacagttttg cttcccgatt agatgaaatt gaaaggttag
    64921 gaccaaaacg aaaaaaaatg ctgcttaaag agttcaaatc gttgaaaaat atcacagcag
    64981 ccacgacaga agaattacaa gaaatcggac tgccgaaaaa tgtcgcacaa aacgtatttg
    65041 ataaattaca tcaggaatga ggaaagggca ctttacaaag aaagtaaagt gtcctatcat
    65101 tttatgtaca acaaaaagag cgtttacgtt ttgttggtaa ataataaatc gcttacgtct
    65161 ggtgtctaag gatatatgtg atttcttgaa gaaggagtga agaaatgtta gaagtcaagg
    65221 atcttgttaa aacttttggg aactacacag ctgtcgatca tgtctctttc caaatccccg
    65281 atggaaaaat catggggcta attggccaaa acggggctgg gaagacaacg acttttcgtt
    65341 tgatcttgga ttttttgaca caagaccaag gagaagtgtt atggaatggc catcatcttg
    65401 gggagaaaga ttatgatgtc atcggttatc ttccggagga acggggactt tatccgaaag
    65461 taacgattca agaccaactg ctttattttg cagaacttag agggaaatca agaaaagaga
    65521 ttgaaccaaa aatcgatttt tggatggaaa aatttcaggt aaaagggaaa aagacggata
    65581 aagtaaaatc tttgtctaaa ggaaatcagc aaaaagtcca gctgatcgcc acgttaatcc
    65641 atgaacctaa attgatcatt ttggatgagc cattcagcgg gttagatccg gtaaatgctg
    65701 aattgctaaa agatggaata ttggaattga aaaaacatgg ttcatgtgtc attttttcta
    65761 gccacaatat ggataatgta gaaaaaatct gcgatcattt gatcatgttg cgaaacggaa
    65821 aaatggtttt gaatggaaaa gtacatgaga ttcgtgaatc atttggccgt acgaagttat
    65881 tcttggaatc tggtttaagc caacaggaag tagaagaaat cgaaggcgtg aaaaaagtga
    65941 tccttcatga agacaatacg ttagagatta ccttagaaga tcctgaagct gggaaagaaa
    66001 tctttactcg tgcgactcag ttcggctaca ttcctatgtt taaccagcaa ccgccaactt
    66061 tggaagagat tttcaaattg aaagcaggtg aaatcgatgc gtaagttttg gattattacc
    66121 aaagatgtct atttaaaaaa tgttaagtcg atttctttct tgatcatgat attagtccct
    66181 tttatcatgc tgggtatcat ttatgtagca ggaaactttg ctcaacaaaa tagtgatgtc
    66241 aataaaatcg gcgtattggc tgataatgag caaatcagtc agcaaatgag ccaagtcaaa
    66301 acagatgatt ttgagttcaa agcagttgat tctgaagaaa aagcacaaaa agagctttcg
    66361 gaagaaaaaa tcgatgcgta tttagtagtc tcaactgaga atgataagat aaatgggatg
    66421 ctttatagcg aaaattcatt aggacaaagt acacaactac tgatacaaca acaactgact
    66481 ggcttgcaat cgatgcttcg agcaagtcaa ttaggcattt caccagaaga agtagctagc
    66541 ttgagccaac aagcaagttt gactcgacaa aaagtcaatt ttgattcaaa aggaaaaatg
    66601 acagttggag aagataactc gacagtccaa tatgtcgtaa gttatgtagc aacgattgtc
    66661 ttgttcatta ttattttgac gtatgcgcag atcattgcac aagagatcgc ttctgaaaaa
    66721 ggcacgcgga tcatggaagt gatcctgtcc agtacgactg cacaaaagca tttttacgga
    66781 aaactaacag gtgttttact tgttgcatta acgcaaatgg cattatacgg tattattttt
    66841 ggtataggat acagtcaatt caaagatatg gaaatggtca aaaatatatt agacggtatc
    66901 tcattggata acttgattgg accattcttg tggtattctc ttctattcat gatctttggc
    66961 attttgattt tttctgtttt agcagcttta tgtggctctt tggtcaacaa agcagaagat
    67021 acagcaaaag caattttgcc agtgacttat ctatctctag gcggatatat gcttggattg
    67081 atactaggag cttcagatcc aaacaatatc gttattcgga tcacttcgta tattccattc
    67141 ttatcttcat atattatgcc ggttcgctta gcaaatgaga cagtcggtat gggtggagca
    67201 ttgatttcct tgttggtact tgtagccgta acattcttat tgatgatctt gtctgcaaac
    67261 atgtacaaat cgaatgtact cgtgtatagc gaaggcggat tatggagttc attgaaacaa
    67321 tcgatttcaa ttatgagaaa tgaacgaaaa aaagcataaa atatagaaaa atatagaagg
    67381 aagctggaca tttattgttc ccagcttctt ttttgtatag acctatttct atttttttat
    67441 tatattggat atatatattc attgaaaata gagcataaca actttataat gttgagtata
    67501 ccaatttttg gaggcgtttt aatggaccgc tatttagatt tactttcaga gaagttccct
    67561 acaacggaag atgtcatcac ggaaatcatc aatctcgaag caattttgca gcttccaaaa
    67621 ggaacagagt tatttttgag cgatatacat ggcgaatttc ctgcttttga tcatattctg
    67681 cgtatcggtt caggaaatct taaagaaaag atcaaagacc ttttctctga acaaatgacg
    67741 gaaacggaga tcagccaatt cactattttt acggcttatc cagaatatgt gttgacgact
    67801 gattggtaca aaaaacaaga taaatcccag ctgatccatc gtttgatcga tcttttacgg
    67861 tttacgacag tcaaatacac tcgatcaaaa gttcgaaaag gattgccgaa agaatatagc
    67921 tatatcattg aggaactctt gtatatcgat gatcgtatca acgggaaaaa agactatgta
    67981 aaaaaaatca ttgaacaact tgtcttgatg aaggaagaag aaaaattttt aacaaaactt
    68041 gcgcagacga tccaaaaatc tgtcatcgat catttgcata tcgtgggtga tatttttgat
    68101 cgaggaactc aagcagccaa agtaatggat cgaatcatgg atttttcctc tgtagatatc
    68161 cagtggggga atcatgatat tttgtggatt ggtgcgtatt gcggctctga ggcatgttta
    68221 ttgacgcttt tacggattgc cgcacgctac aactatttat ttgaattaga gaaagaatat
    68281 ggattaaatt tacgtccatt attttctttt gcccatcaaa cctatcagaa gaatccagta
    68341 tttaccccta aaaacaggaa agatctatct gaaagagagc aagaagaatt agaaaagatc
    68401 catcaagcac tttctatcct ccaattcaaa tcagaacatc agttgttgaa tcgacgacct
    68461 gaatttaaaa tggacgatcg taaattgttg gaaaaaatca actatgagga atcaacgatc
    68521 gttttagaag gtcagctcta tgagttgaaa gggacttgtt ttcaaacgat ccagccagat
    68581 gatcctaaaa aattcttgga agaggaacaa aaagtgatcg atagtttgat gtattcattc
    68641 cagcactcgt taagaatgca aaaacacatg acgtttttga tcgaaaaagg gggaatgtat
    68701 ctcacgaaca atcagcatgt actgttccat ggctgtatac ctgtagatga aaaggggcag
    68761 ctcttagcat ttgaattaga ccaatcttat cgcggaaaag aactgctggg attttttgaa
    68821 aaacagataa ctgaaagcgc aaaagatttg actgcaaatg aagatttggc gaccgatttg
    68881 atttggtatg cgtggagcgg gccattttcg cctttattcg gcaagagtaa aatggcaact
    68941 tttgagcgct attttctcac agagcctcat acgcacgtcg aaaaaagcaa tccctattat
    69001 cacttgcgag atgaagagtg gttcagtgaa aaaattttgg cagaattcga tgcaaaagaa
    69061 gaaggttcag caatcatcaa tggacacacg ccagtaaaag taaaaaaagg agagtcaccg
    69121 atcaaagcaa acggaaaagc atttgttatt gacggaggtc tatccaaagc ttatcaaaaa
    69181 acaaccggga ttgctggcta ctcattacta tgcaattctt acggtttcca aatcgtgaca
    69241 cattatccgt tcttgcctat tgaacaacaa tttgcaaaaa aatctgatca aacgaatgta
    69301 aaaaaagtga tcgagcagca gctcccacgc aaacttaatc ggaatacgac aaaaggaatg
    69361 gaattacagc aacagattac tcaactccaa aggctgttgg attatcgaaa ggacgattga
    69421 gaaaaatgct agccaacatt gctcaataga ctaaaaagcg actgcaacaa aaggcgtgtc
    69481 gcagtctcct tcggaattag ctcaaaaaac ggtaaaatat tagaagctat ttttctgttt
    69541 atttctctaa tactcaggtc agggcgctga tttcacgacc tcttccttaa acggtgttct
    69601 cctgccaact cctcggtcac aagtcacaat atccaacaaa catgagaagc tgttttttga
    69661 aattgctcct tgtgactcag agttgaacgg caggctcacg acctaatcag aaaaacgatt
    69721 aaaattcatt cacaaagaaa tcgaataaac gctgttcaga tgaatctaca cgatgagaaa
    69781 gttccggatg ccattggaca ccaaggattt tgtgcatatt atctttactt tggactgctt
    69841 caatgagtcc atcgttggaa aaggcgatgg cttttaacaa aggagaaaga tctttgactg
    69901 cctgatgatg ataggaattc acaaagtatg aatcagggag aaggtcactt aataaactgt
    69961 ccgaaacgat tttgacttcg tgagttgcaa actgcggggc agtgggctgt tgttcgtgtt
    70021 ttacggacca ttttggatat aatgaaagat cttgataaag tgttccctcc aacacgacat
    70081 tcaatagttg aagtcctcgg cagactgcaa aaatcggttt tctttgtttc aatgcttcca
    70141 agatcaatgc ctgttcgaat tggtctcgtt gaatatttgt ttccgtcagt tttggatgag
    70201 ggtcttccat gtaaagttgc ggactaacat cctgaccgcc tgcaagaatg agtttgtcga
    70261 ttttctcaat tagttgaggc gctgaatctg gagacatgac tggcagaata agaggtaatc
    70321 cttgagcatc taaaactgca tccacaaatc cctgcggtgt atagcttact tgatttcctt
    70381 ggaaagtatc tgtcgctcga atcagttgat tcccggcaat gccgatgatt ggtttcattt
    70441 ttattccccc taaagtagac taattgtaac aaggatttaa aaaaatttaa actatgtaag
    70501 acttgcccct atacttatct gttcttgctg tgttactatc tgtaaagaga attttcgtgt
    70561 gaaattcatg acgatagttg aaaatattgc ttaatttgtt ataataaaaa gtaagcagaa
    70621 ataaaaaggt tgggcgtaag cttcaaggat ctagttcttc aaggggtgag aaggacattt
    70681 tattgaaggg gcctgcctat actaagttat ctttagaggt acatactttc aatgaaaaaa
    70741 gaaagcatgt acctcttttt taaatcgaaa ggaagtgaag caagtgttag aagctttgga
    70801 acaaatccga aaagcagaag aagaaaatga gaagcgtgct cgaaccctac ggcagcagtt
    70861 gaagacctat gcagaagaaa aagagcaaga attggagata atccaaacga acagtcggca
    70921 acagctgaaa cagttggtag aagaaaaaga acaattcgaa aagcaagaat tagaaaaaga
    70981 aaaaatgaaa ttgaaagcag aagcagacaa ggtgcaagca actttgaaag accaatatca
    71041 aaagcatcag agtcaagcaa tcggtgcaat cattgaaagg gtgaaagaaa cttatggccg
    71101 tcactaagat ggaaaaagtg accctcattt cagataaaag aaatcaggag aggattttac
    71161 aagcagtaca aggaatccaa aatgttgaaa tccgggatct gtatcaaaat agaacaaaca
    71221 accagtgggt agaaaaatat tttccagata ccacgattat tgataaagaa gcaaaattaa
    71281 gcgctttagg caatcggctg aaagacatcc aagaagctat ccaattcata caacaccacg
    71341 gcgatagccg tcaaaagaaa ctccatttga aacgtcaaga actttcctta caagacttgg
    71401 aagcaaatta ttccgaagaa aatttcatga ataaattaga agaagtcctt gatttaaaag
    71461 aaaagtggga acagctaact gaaaaacgtg atcgtttaga agaagaggaa gaatggcttc
    71521 ttcattggca acagctggat attgctccaa gtatgtatgc aagtaagacg accatttttg
    71581 agatggggac tgtgagtgca gcaaattttg aaagtttcaa ggaagaacta agtaagcttg
    71641 atgacgttta tatggaagaa atagattata cgccaaaaca agtttatctt tcgtatgttg
    71701 ctttaaaaaa atcagttgct gctatagaag aaattgctgg gaaatacggg ttcaataaag
    71761 aaacgtatcc ttatgaacaa aatcctaaag aaaaattgca agaaaccaaa cagcaattat
    71821 ccattgttca cgagcagcaa aaaaagataa cgacagcgtt aggtcagtgc agcggctata
    71881 tccaagaatt tgaatgggta gaagaagtca cacttgccgt tgcagaacgg gaaaaagtaa
    71941 aagaacggtt tatccatgcc acttatctga tcgttttgca aggttgggta gatgcagaag
    72001 aaaaacagaa tttgatgcat gtgttaaacg aaactgtatc agaagaagat atatacgtta
    72061 gtttcgaaac accaaactca gaagagattc agcaggaagt ccctactaaa ttgaaaaacc
    72121 atccgcttat cgaacctttt gaattgctga ctgaaatgta cagtctgcca aaatatgaag
    72181 aagtagatcc aacgccttgg atgacaccat tttatcttgt cttcttcggg atgatggtcg
    72241 cagatgttgg ttacggattg ctgatgctca ttgggacaat actagctcaa aaactatttg
    72301 ttttaccccg cgggatgaaa cgtttcgtaa aattctttga gattctctcg gtaccatcga
    72361 tcatctgggg attgatctac agttcatttt tcggacaggc attaccaaaa gaactatttg
    72421 gtatccgctt gcctttccca atcctatcta cgacggatga cgtcaatacg attttgattt
    72481 tatccgtgat tttcggtctg atacagatct tggttggatt attcgtggca gcaaaggaaa
    72541 atatcaggag gaaagattac ttgagtgctg tcagtgatgg ttttgcctgg caagggatcc
    72601 tgattgggat cgttattgca ttgattggtg tcatgttgat aaaaaataca gcatttgttt
    72661 atcttggcgc ggggctatcg atcattgctg ctttatgtat cttaatcgtg ccgattatcc
    72721 aatcgccgtc taaagtcaag ggtgctgcca aaggagcgta taatctttac ggattgactg
    72781 gttatatcgg tgatttagtc agttatacac gtttgatggc actaggtatt tcaggcggaa
    72841 gtattggcgc ggcattcaat atgctagttg ctttcatgcc gccagccgct cgattcagtg
    72901 tagggatcct tttgatcatc gctttacacg cactgaatat gtttttgaca ctgttaagtg
    72961 cctatgtcca tggtgcacga ttgcagtatg tcgaattttt tggaaaattt tatacaggag
    73021 gcggacgtgc ctttgacccg ctgaaaacgg cggaaaaata cgtcaacatc aatcataaga
    73081 aaaaaaacaa taaataattg gaggattttt atacaatgat ggattactta attagtcaaa
    73141 atggtggaat ggtatttgca gtattagcga tggcaacagc gacgattttt tcagggattg
    73201 gttcagctaa aggtgttgga atgacaggtg aggcagcagc agcgttaaca acaagtcaac
    73261 ccgaaaaatt cggacaggct ttgattctgc aattgcttcc aggtacgcaa gggttgtatg
    73321 gattcgttat cgcctttttg atttttatca acttaggaaa tgatatgtca gttgtacaag
    73381 gattgaactt tctaggcgct tctctgccga ttgcttttac aggtttgttt tcaggtatcg
    73441 cccaagggaa agttgcagca gcaggtatcc aaattttagc gaaaaaacct gaacatgcta
    73501 caaaaggaat catttttgct gcgatggttg aaacatatgc gattttaggt ttcgttattt
    73561 ccttcctatt agtattgaat gcctaagcat aaaggaggaa aagcagatga acgccattga
    73621 aaaaatcatc tctcagatga atgaagcagc agagcaagaa agagcggcgt tagagcaaga
    73681 agagcgaatg aagatcgatc aaaactttga acaaaaacgt acgcaaatcg aaacagaaca
    73741 tcaaaaacaa aaagaaaaac aaatcgaatt acttgaaaaa aaataccgtc agcttaggaa
    73801 tcgccaacaa gtagaagtac gacaagaaaa tttgaatgcg aagcaggaat ttttacgacg
    73861 attgtttgca gatgcaatag ctgaaatgga gaattgggat gaatctgaac aaatccagtt
    73921 tatcaaaaat tcattgtatt cattaccttt gacaggcaaa gtaacattca ttgctggaga
    73981 aaaatcagca gcttatcttt ctcagacatt gctggatgaa tggaacaatg aacttccctt
    74041 tatgatggtt ttaagtgatg aaactgttgc tgatcaagcg ggttttctga taaacgatca
    74101 aggagttcag tataattttc ttttcagcag ccttgtacaa gatatccaag gaacgatgag
    74161 ttttgagatt gccaatcaat tattcgaata aggagggatc agtgtatgga ataccacgaa
    74221 ctaaatccct tgattcgcgg aagagagctt gaacttttgt ccaatgagac atttgaacgg
    74281 atgattcaaa caaattctgt cgaagcactt ggtgaaatat tgaaatccac gatttacagt
    74341 ccatatattt atgacggata tgaatatgac ttcgaacaga atcttttaaa ggagcagagc
    74401 cgtctatttc gctggttaaa ggaatcagca ccggagcctg aaatcgtttg gatctatacg
    74461 atgcgctata ctttccataa tctgaaggtt ttgacaaaag ccgagatgac aaatcaaaat
    74521 ctggaccatt tgtatatcga cgatggattt tattcattag aaaccttgaa ggatgccatt
    74581 catacgcaag tttcaaatga actccctgaa agcattatgg acagtatacg agaagtacgt
    74641 gattattttg aagagtctac cattcttcag ggaattgacg tgatttatga tcgacagttt
    74701 ttgacagagc agcgacggct tgccgataag ctaggatatc aagaattgct gacagaagtc
    74761 attgctttta ttgacttgac caatatcaca acgatggctc gaggaatatt gcagaaaaga
    74821 acatatgggt tcatgacgac ggtactttcc agctcgggaa gtatcgaaaa agaagtattt
    74881 ttgaactttg tagataaaga tatggaagat tacacgaatt ttctattaga gacagattat
    74941 gctgattttc tgcgccctgt catccgtgag ggaaatcttg atctcgtaaa gcttgaacag
    75001 ttgaaagatg attatctaag ttcattgtat caagaagcac aaacccaggc tttcggacca
    75061 ctgccactac tagccttttt gaacgcaaaa gaggtagaaa gcaaaaacct tcgtttactg
    75121 gttatcggca aaagaagcca tttcgaacca gaaacaatca gaaagcgggt gagacaagtc
    75181 tatgacgcat aagatcggtg tagttggcga caaagattct gttttgccat tcaaattgtt
    75241 tggctttgat gttcgttatg gtacaaccaa gcaaaccgtt cgtcaggcaa ttgaagaaat
    75301 ggcaaagaat gagtatggcg tgatttatgt cacagagcaa tgtgcagcat tgagcccaga
    75361 aacgatcgaa cgttacaagg aacaaatgat acctgctatc gtattgattc ccaatcatca
    75421 aggatcatta gggatagggc tggaagaaat tcaaaaaaac gtagaaaaag cagtaggtca
    75481 aaatatctta tagtatgaaa aggagataga aattttgcaa attggaaaaa tcataaaagt
    75541 ctccggacct cttgtaatgg ctgagaacat gtctgatgcc agtatccaag acatgtgtct
    75601 agtgggagat ctaggagtta tcggagaaat tatcgagatg cgtggcgatg tggcgtctat
    75661 tcaagtatat gaagagactt ctggaattgg tccaggcgaa ccggttcgct ctactggtga
    75721 agctttatct gttgaattag gaccagggat catttcacaa atgtttgacg gaatccaacg
    75781 gccattagat acctttatgg aaatcacaca aagtaatttc ttagggcgcg gagttcaatt
    75841 gcccgcattg gaccatgaga aaaaatggtg gttcgaacca acagtagaag ccggagaaac
    75901 ggtgagtgct ggagatgtta tcgggatcgt agaagaaaca aaagttataa aacataaaat
    75961 catggtgcca aatgggataa aaggaacgat caaaacaatc gaaaaaggtt cattcactat
    76021 tgatgaaaca gtatgtgtca ttgaaaccga aaatggtgac aaaaatttga cgatgcttca
    76081 aaaatggcca gtaagacgta gtcggccaat caaagaaaaa ttaaacccgg atgcacccat
    76141 gatcactgga cagcgagtga ttgatacatt cttcccagtg acaaaaggag gagcagcagc
    76201 tgtacctggt ccttttggtg cagggaaaac agtcgttcag catcaaattg caaaatgggc
    76261 cgatgtggac atggttgtct atgtgggatg tggcgagcgt ggaaatgaga tgacggatgt
    76321 tttaaatgaa ttccctgaat tgattgaccc taatacagga gaatcgttga tggaacgtac
    76381 ggtattgatt gctaatacat caaacatgcc agtagcagct cgagaagctt ctatctatac
    76441 gggaatcaca attgcggaat atttccgtga catgggttat gacgttgcta tcatggcaga
    76501 ctccacttca cgatgggcag aagcacttcg tgagatgagc ggacgtttgg aagaaatgcc
    76561 tggtgatgaa ggttatccag cttatcttgg ttcacggctt gcggaatact atgaacgatc
    76621 tggaagagtc attgcattag gatcagaagg tcgcgaagga agtataaccg caatcagtgc
    76681 tgtatccccg tctggagggg atatttcgga accagtcaca caaaatacat tgcgggtcgt
    76741 aaaagtattc tggggtcttg attccagttt agcccaaaaa agacattttc cttcgatcga
    76801 ttggatacaa agctattctc tttattcaac agaagtaggg aagtatatgg atcatatcct
    76861 tcaagctgac tgggcatcca tggtcaccga gggcatgcgt attttgcagg aagaacagca
    76921 gctgaatgaa atcgtgcgtt tggtagggat tgattccctt tcagataatg accgtctgac
    76981 attagaagtg gcaaaatcca ttcgtgaaga ttatttgcag caaaatgctt ttgatgatgt
    77041 cgatacattc acttcaagag agaaacaatt caaaatgttg aagatgatct tgacctttgg
    77101 tgaagaagga cgccgtgcgt tgtcgttagg tgcttattta agtgagatca tggacggtac
    77161 cgtatcgatt cgtgaaagga tcggacgaag caaatatatc ccggaaacag aattagaaaa
    77221 actagatcaa attattggtg aaatcaagca aacgatgcaa acgatcgttt cagaaggagg
    77281 catgagcaat gattaaagaa tatcgtacga tcggtgaagt cgtcggtcca ttgatggcgg
    77341 ttgaaaaagt tgctggtgtc aaatatgaag aattaatcga agtccgtatg caaaatggcg
    77401 agattcgtcg tggacaagta ttggaagtcc aagaagataa agcaatggtc caaatattcg
    77461 aaggaacaag tggtatcaat ttacgtgact cttctgtacg atttttaggc catcctttag
    77521 aactaggtgt atcggaagac atgatcggtc gagtattcga tggtttggga cgaccgaaag
    77581 ataacggacc agagatcctg cctgaaaagc atttggatat taacggagaa gtaatcaatc
    77641 cagtagctcg ggattatcca gatgagttta tccaaacagg tatctctgca atcgatcatt
    77701 tgaatacatt agttcgtggg caaaaattac cagtattttc tggctctggt cttcctcata
    77761 aagaattagc tgctcagatc gcgcgacaag caacggtact cgattcatca gatgatttcg
    77821 ccgtagtctt tgctgctatt ggaatcactt ttgaagaagc tgaatttttc atggaagatt
    77881 ttcgtcaaac gggcgcaatc gaccgttctg tcatgtttat gaatttagca aacgaccctg
    77941 caatcgaacg tatcgctact cctcgtatgg ctttgactgc tgcagaatat cttgcttatg
    78001 aaaagggaat gcatgttttg gttatcatga cagacatgac caactatgca gaggcattgc
    78061 gagagatttc tgcagcacgg cgagaagttc caggaagaag aggatatcct gggtatcttt
    78121 acacgaactt agctacttta ttcgaacgag caggaagaat ccgcggacta aaaggatcgg
    78181 ttactcaaat tccgattcta acaatgccag aagacgataa aactcatcca atccctgact
    78241 tgactggtta tattacagaa ggacagatca tcttgactag agagctttac aagagtggga
    78301 ttcagccacc aattgatgta ctcccgtctc tttcgcggtt aaaagataag ggaactggac
    78361 ccggaaagac aagagaagac catgcagcta cgatgaatca gctttttgca gcctatgcac
    78421 aaggaaaaca ggcaaaagaa ttagccgtag ttttaggtga atctgcttta tctgatgtag
    78481 acaagatcta tgcgaagttt gctgagcggt ttgaaaaaga atatgtcaat caaggattct
    78541 acacgaaccg ttcgattact gaaacacttg atctagggtg ggaactgcta tcgatgttgc
    78601 caagaaccga attaaaacga atcaaagacg acatgctgga caaatatctg ccagaaggga
    78661 agtgagttct gatggcacga ttgaatgtga atccgacccg catggaattg actcgattga
    78721 aaaaacagtt gacaactgcc acaagagggc ataaattatt aaaagataag caagatgaac
    78781 tgatgcgtca attcatcctg ttgattcgaa agaacaacca gctgcgtcaa gaaatggaag
    78841 agtctatgac agaagcaatg tctgattttg tattggcaaa tgcttcgatc acggaacctt
    78901 tcatcgaaga attatttgtt ttgccggcgg aaaacgttga tttgtctatc gtagacaaaa
    78961 atatcatgag tgtaaaagta cctgtgatga attttgaata tgatgaacag ctgacagaca
    79021 cacctttgga atacgggtat ttgaattcca gtgccgaact ggatcggtca atcgatcggt
    79081 ttactaccct gcttccaaaa cttttggaac tgacagaaat cgaaaaaact tgtcaactga
    79141 tggcgagtga aatcgaaaag acacgtcggc gagtcaatgc attagaatat atgacgatcc
    79201 ctcaattaga agaaacgatc tattatattc gtatgaagct ggaagaaaac gagcgcgcag
    79261 aagtaacaag gttgatcaaa gtaaaaaata tgggtaccac tgactaagtg gtatcttttt
    79321 ttaaaagagg aaaacgatga ttaaacaaag caaacaaata aagaaacgtt tgccggctgt
    79381 tcagcttctc gcattaggat tttttctcct gattttagtt ggcggcggcc tacttaccct
    79441 accatttttt agtaatagtg ggcatgggac aaattttatc gatgcgcttt tcactgcaac
    79501 ttctgctgtc tgtgtaacgg gattgacaac attgaataca gcagagcatt ggaacagtgc
    79561 tggtcaattt ttgatcatgc ttttgattga gatcggcggt cttggtttta tgatggttcc
    79621 gattctatgc tttgcattga taaagaagaa agtcagtctc agcacacgta ttatattaaa
    79681 agaagcgctg aatctagaag aagtatcagg tgtgatgaag ctcatgcttt acatcctaaa
    79741 attggccttt gttatccaat tgattggtac ggttgcttta tcttttgtat ttgtgcctga
    79801 atttggctgg ttgaaaggta tctggtacag tttgttccat gcaatctcta gtttttgtaa
    79861 tgcagggttt gatttgttag gagacagttt agccaatcac cagcaaaatg tttatttgat
    79921 catggtggta tctgctttga tcatttctgg tgggttaggc ttcatcgtat ggcgtgattt
    79981 actggaatat catcagaaaa agaagatgac gattcattca aaaatcgcat taaccgttac
    80041 aggtaccctt ttattaggcg gattcgttgt gttctttttc acagagcgca atggggcaac
    80101 gctagtggaa gggaatatgt ttcaacgatt ggcaaacacc ttttttatga gtgtgacacc
    80161 tagaacggct ggatattatt cagttgatta cctccatatg agccatgcag gtctgatttt
    80221 aacgatgttt ttgatgtaca tcggcggaac atctgggtcg acggccggcg gattgaaaac
    80281 aactacgtta ggtgtgttgc tgatccagat gcgttcgatg ttgaaaggac gtacgagagc
    80341 ggaagcattt ggccgtacca tccgacaagg cgcagtgttg cgggcactta ctttattttt
    80401 tgttacgttg acgttgtgtg tagtcgccat catgatttta tcggtgactg aaacgatacc
    80461 taaaacttct ggcatcgaat atattgcttt tgaggtcttc tctgcgtttg gtacagtagg
    80521 tcttacgatg ggattgacgc cagacttgac tgcaataggg aaattgatca ttatagcgct
    80581 tatgtacatt ggacgagttg gtgtattgac tgtggtcttc tctctgatga caaaagcaca
    80641 aagaacacaa tcaaaataca aatatccaga agaaagtgtc atgattggat aatttcatct
    80701 tctgacagaa acctagtgat ttagtaaaat aagaaaataa aataattttt cttgcttctt
    80761 ggatacttta agtgcaaaat gttcgtttat tttttgaggc actacccgtt gttgaacctc
    80821 gggtagtgcc tcttttttat tgaaaggaat caataagatt tgattccttt atttatcaat
    80881 aggttataat tcatattttt ttattttttc ttttacgata cttagtccag aagctaaaat
    80941 ttcaaggtta ttagaggata gggaaattcg aacgaatgca tctggttgcg cacgtgtagt
    81001 aaagcgtctg gaatgataaa gtcggatacc gttagcaaga aagtcattct caattatctt
    81061 agggtcaatc tcaggatcaa ctggaagaat tcgatagtaa gggacacgat tcgttggttt
    81121 ttctactttg aaaagagaat caaaaagctg attggctctt tttgtaagac ttaatttagt
    81181 ttctaagatc ctatttgctt catttgaatg gaggatttga gtgatgactt ccacatctaa
    81241 tccagaagtt ttcacattta tattaaaaat tgcttgttct atcttgcttt ggacatgtac
    81301 tggaactaca agataagcaa ctcgcaatcc agaacaaata aattttgtca ttccagctaa
    81361 gtagattgtt tggttaggta ataaattttg gaaaggagga atgatcgtat gttgatagta
    81421 agttgttaaa aatgaatgga tatcatcttc aataacccat agaccttctt tttcaatcac
    81481 ttcggctaat tgctctcgtc ttttgtgaga gatttgaaac cctataggat tattacaaga
    81541 aggcataaga aagacaccat gaatcttctt ttttctacat tcctgaatca atatatcagg
    81601 tcgcattcct tgctcgtcaa aatcaattgg aacgatctct aaatgatata actgagccaa
    81661 ttcaatgaaa ttagcgtatg tatatcggtc tgttgctatt ctatcacctg gagagaaaag
    81721 tgcagaaagt gtgatagcca atccattttg aacaccggaa acaatagata gagattctaa
    81781 ggacggaatg actccttgag tttttaacca atttgctgca gtagtcaatt gaaaatttgt
    81841 gcctttggga tcttggtaat taagcaaacc aacaagattt gggttattac tagttgattg
    81901 aataaacggg acaacgaatt tattacattc ttcaaaagaa ctaaccagac caaaatcaat
    81961 gatatgttcg gttacttcat tggtagagat ggtgatggac tcaaagacat tgggagaaat
    82021 aaatgtgcca cttccaatat tcgtatacag caaacctttc tcaattccat atttataagc
    82081 cttgccaacg gtagtaaaat ttaaatctaa ataatcagct agctctcttt gggagggtaa
    82141 ctgagtgttt ttctgtaatt tattctttaa aatatcttgt tctaattgtt gaatcaacga
    82201 tcgataataa ggacgcttca atttttgttt gtctggtcgc caagttaacg ggtaattgtc
    82261 ataagaatta ataggcattt tctccactcc ttaaaattgt ataccataca attttaccat
    82321 tgattgtatt gattttagaa gttaatcttt tgtgaggagg gaattgtcta tgaaaattat
    82381 taattttgag gaagtagtag cttcttttag ttttgaagaa gcgattcaag taatgaaaaa
    82441 atgttttttt gattatgaaa gtggtaaaat cagccaaaat cctcgattgg tggaaatttt
    82501 acctgacgga aaaggcaaaa atatgtttgc gatgatgcct gcttatttgg gggagaacag
    82561 atattttggc acaaaagtca ttaccgcttt tccggataat catcagtatc atttaccttc
    82621 tcatttagga gagattttct tgtttgatgc tcaaaacgga ctacccaaag cattgataaa
    82681 cgcaaatgca gtcacctgga ttcgaacagc tgctgtatct gcgctggcaa cagattactt
    82741 agcaaatccg catgcagaaa cacttgcttt aattggagct ggtcaacaag cattgtcgca
    82801 tttgactgcg atttctacca ttcgtcccat cactacagtg tttgtttacg atattttaga
    82861 agaaagacga aatactttta tcgctaaagc taaaaaacag tatcctaaaa taacgtttat
    82921 aaatgcaata tcagtagaag atgcagtacg tccagcggaa atcatttgta cgttgacctc
    82981 tagtaaagag gcatttcttc aaaaagagtg ggtaagggaa gacgcacata tcaatgctgt
    83041 gggcacattt actcctgata caagagagat aagtacatcg ttgatggaaa cttctcaaat
    83101 ctatgtagat gattactcag ctgccttaac agaaagcggg gacctgctta tagccatggc
    83161 agaaggagat ttagaggaat cttgtattgt tggtacatta ggcgaattag tttctgagaa
    83221 aaaaacattt tataaaggag aatcgaggcc gacgattttc gacgcagttg gtctagctat
    83281 tgaagatctt tgttgtgcag aatatattta taacaaatta gagaaagggg ctttaaaatg
    83341 aacttagatt attacgtagt tgatgcattt gcagatgagg tgttcaaagg aaatccagca
    83401 gcggtttatg tgctagaaga atggctgcca gaaggtacga tgcaaaaaat tgccattgaa
    83461 aacaatttat ctgaaacagc atttacagtg aagaaaaacc aagaatttga gcttagatgg
    83521 tttacgccag atcgagaaat cgatctttgt ggtcacgcga cattagcaac agcatttgtt
    83581 ttatttaact attacaaaat acctgatgaa acgattaaat tttctactca aagcggtaat
    83641 ctatttgtga caagaattaa agattattat tacatggatt ttcctagtat tatgccgaaa
    83701 aaagtgccga tattgtctga atatgaagaa gcgattgggg ctaagattaa agaagcctac
    83761 ttagcacgtg atttattttt cgtattagaa gatgaaaaaa cggtggctaa attgcagcca
    83821 gattttacag ctatcaaaaa ttttgaatta ggaataggag tcatcgtgac agcagaaagt
    83881 gtgcaaaaag actttgtttc tagaaccttc tttccaaaat taacaatcaa tgaagatcca
    83941 gtatgtggat cagcacattc taatcttatt ccttattggg cagaaagaat gaataaaaca
    84001 aaattgtcag cttatcaaat gtcatcaagg ggcggggatc tagaatgtga attaaaagaa
    84061 gatcgtgtga taataggagg aaaagcagtt ttgttttcta aaggtacctc gtttatttga
    84121 tttctttcaa atgaattaaa aataaattgg tggagagtgg caaccttata gctacaatgg
    84181 ttgttaaaaa aagacgcaaa ctttttcaat ttaatgaaaa gtttgcgtct cattttaatt
    84241 attggtgttt gttttttatt caatcaagag taaaatgccc catactaact atcctcggat
    84301 gacttcgatt ttataaccat cagggtcaat gacaaagtag taagaaggcg cagtgccagg
    84361 taatcctttt aagtcagtaa tctcaagacc ggcagcttta tgttgctcgt gtaatttttc
    84421 tagatcatca gctgctatag cgatatgacc atagccgtct cctagatcat acgcaccatg
    84481 atcatagttg taagtcaact ctaattcata gtcatcccca ggaagtgtca aataaacaag
    84541 tgtaaatttg ttttctggaa aatcacggcg gcggctttct tcaaaaccaa atgccttttg
    84601 ataaaattcg attgatgctt caagatcttt cactcgaaca catgtgtgag ccattttcat
    84661 agattcatct ctccttttcc ttttactcaa tcataacaaa aagaagtaag gaaatagttg
    84721 atttgtgctt gttcatgaga aatttctcat gtaaaatgga tagaaaataa aactgttaag
    84781 gtggaaacta ttcatggata aaccaatttt tggtgaaaaa aagcaatttg ctgattatca
    84841 aacccgttat gcagcatata ttgtcataga aagagatcag cagattgcag taatcgaggc
    84901 tccaaatggt gctttttttc ttcctggcgg ggaaattgaa ggagaagaga cgaaagaaga
    84961 agcgattgtc cgtgagctga tagaagaaat gggaattgtg gcagaaattt cttcttatct
    85021 gggagaagct gatgagtatt tttactcgaa ttatcgtgca acttattatt ataatccagg
    85081 atatttcttt gttgctgatt cttggaagcg tataggagaa ccaactgaaa agaccaataa
    85141 aatttggtgg gtgagccctc aagaagcgct tgtgaaattg aaacgtggta gccaccgatg
    85201 ggcagtcgag agatggatcg aaaaaaataa aaactgaaag aaagcgagta gatcagcttt
    85261 ctctcagttt ttttatttat tacagtacat attttttgat tgcttcagct acaccgtgct
    85321 cgtcgtttga tgaagtgatg acatttgctg cttctttgac aagagaaacg gcatttccca
    85381 ttgctactcc tagaccagca tattcaatca tggataagtc gttctcgtta tctccgatag
    85441 ccatgatttc atcttgatca ataccaaggt gctgtgcgag attcgcaact gccgcacctt
    85501 tacttgcttc tttatttagt acttcgaagt aatagggcgc acttttgacc gttgtgtatt
    85561 tatcacggaa agtttgcggt aaacgcgcaa ttgctgcatc taaaatttct ggttcgtcga
    85621 tcatcatcat tttgacgatg ttcatgtctg gtgtcatttc ttcaggtgtt cgatatttta
    85681 gcggcatctt gactaaactt gcttcgtgga tcgtataagc gctgatatct cgattaggcg
    85741 tgtagatcgc tttgtcatcg atcgaatgga gatgactgcc gacacggcgt gccatcactt
    85801 cgatctctag gaaatcttca tgtgtcagtc cgtgacgggc gatgatttta cctgatttcg
    85861 tttgttgtac gagtgccccg ttataagtaa taacgtaatc attatcttga aaaaggttca
    85921 actcgtttaa ctgttcctgg acacctggta agggacggcc agtgcataaa acgatcgaaa
    85981 caccttttgc aacagcagcg gcaatcgctt cttttacttc ttttgttact tctcttttct
    86041 catttaataa agtaccatcg atgtcgatag cgactagttt gatagacata atttcctcca
    86101 atatatttta atttatttca atcaatgcgc cattacggat atggctggca aattcttgat
    86161 aagtttcgtc aaataaatca taatgatcac gcaaggacgg atcaaccatt tcttttggaa
    86221 agaaaaatcg ctcatctcct tgttcttgtc ccgctaatgc agcaacaagc tgactaacct
    86281 gtgataattc tatcaaagtt ccgtcatttc gcaaaatctc gatttgtgtg cgatgtctgc
    86341 cttgttctgg gcgataaaaa tcataaggta gatcgtagct agaattcaca gcagtgtaat
    86401 aaacaggatt atatccgacc ttttctacta acaaagtgag ttcttggact aattttgaat
    86461 cccggttgcc agaaaaagta gcagatttca gtggtttacg attcaaaaaa cgtttagcta
    86521 aatcatttaa aataggatca gcagattcag accattgagt aaagtaagtg cccaagaccc
    86581 catcatcaag tttgaggtat tcttctaaag taaagcttcc gtcaagaaat ggcagaagta
    86641 attgagaatg gagttcaaaa ctgctggcat cttcttgata caactcgtgt gcgcgatgaa
    86701 gcagatgctc caaaattact tccataccac gggaaacagg atggaagtac acttgcacat
    86761 acatttgata tcggctaacg atataatctt ccactgcatg catgccactc atggaaaaag
    86821 cgatcccgcc ttcataagga cgaatcacac gtaatattcg ggtaagatca aatgttccat
    86881 attctgttcc agtgaaataa gcatcccgaa gcaaatagtc cattcgatct gcatcgatct
    86941 gactagaaat catttggaca acttgtgggt tggggtaagt tttttggatc acactagcta
    87001 ccttttcagg aaaaccttct tctactcggt taagaatctg atacacttct gtttctggag
    87061 aagtgatgat ctctacagtg atggcttcat gattcgtatg gaagatatgt tcaaatgtat
    87121 gagagtaagg accatgaccg acatcatgca acagtgccgc acataaagtc accagccgtt
    87181 cctgatcatc ccagcctccg ttgcctattt tttcttttga aaaatttcga gaaaagatat
    87241 cgcagattcg tcttgaaatc tcataaacgc ctaatgaatg agtaaaacgg ctatgttctg
    87301 ctccgtgaaa agtgaaagaa gaagtgccta attgtttaat gcgtcgtaga cgttggactt
    87361 ctttcgaatt gatcaaatct aagattactt gatgctggac atggatatag ttatggactg
    87421 gatcacgaaa aactttttcc ataggcagca tttgatattt ataaggggtt gtcataatgt
    87481 agctccttta tgttattacg ttgttccatt ctctggattt ggttgttcca ttgctccagc
    87541 tggatttctt tttttagcca gtcggtagaa tcctgtaaat cacttgcaga atacagcttt
    87601 tcaaaagctt ttgcaaatgc ttctttgact tcttttacaa aaaggggttg gtcaagcagt
    87661 tcttcaagtg ttgccattgt agcaggatcc actgcaggat agccatttgt accaaagtct
    87721 tcttttaaag cagattgata aaatcgcttc atgacttgcc cgcgtttttc ctgattaccg
    87781 ttgacactta agtacatcat aacagaaatc ccatttttga tccgacgctg agcgataccg
    87841 gcaaattttt tgccatggat gcttaaatca taagttccag gacaataaga atgggtgatt
    87901 tcgaatgctt gtatcggtgc ctttggaaaa gcgtatttag tcaataggag catttgctcg
    87961 taagcgtcat ctatggttat tttttttgtg tcactttggg ggaaaatcaa agaaatattc
    88021 aaaattcctt catctgatac gatccctaat ccacctgaat ttctgatcac tggagcgtaa
    88081 cctgcatctt tcagtgtttg gatcccgtca cttagaaaag gagttcgaac atccttcata
    88141 cctaagatga ctgtgcagtc atattgccag aaatgtaaaa agatttgctg gtgttcttta
    88201 gaatattccg ttaatatatc tgttaatgca aaaggggaaa agtcgttttt ttgataaatg
    88261 tgctgatcta gtcgaatcgc agggttcatt caaccggctc ctttgtataa attcatattt
    88321 tctacaatat tatactctaa aatcaaagag ttgttttgga aaaaaggttt tctgtaaaaa
    88381 tggacaagtt ggttgacaaa acaaaagaaa ctaggaacaa tgtagctatg agaagtaaag
    88441 gagaatgaat aatggaatta tcacaaggaa caccgatttc aatcaaattg cgtacaaaag
    88501 taaaacagca aggggaagtg caagattttt attttgattt gaaagggcaa atggtgacaa
    88561 ttggcgatac gctgtacata cgttataaag agattcagga ggagacgggc gatgagattc
    88621 ctgtaacgat caaattagtt ccagatggtc atgtacagct gatccgtgca ggagaaatgc
    88681 ggatgcgttt gaaatttggc tataaagaac ggttagaaac atcctatcga acaccatacg
    88741 gtatgatcca gattgctact tttacgaaag aactgcatgt tagtttgaaa gaccgtccca
    88801 caggagggaa ggttcgaatc gattatgatc tgttcatggg accggaaaaa atcggcgaat
    88861 attatttgac cttggatttt acagcgtaaa tataaaagaa aagtttgatt ttttgtgtct
    88921 gattttgtat gataaggagt agactgtgaa aggacgtgtc ctgattggaa attagtgtat
    88981 ttgaaggtgc aaacaaaagc gaattatcaa tgatcgaagt agcacacgcg atcttagaac
    89041 aacgtggcga tgtcatggat ttttctgatt tagctaacca gatccaaaac tatcttggga
    89101 aatcagacag tgaaattcgt gattcactcg cacaatttta tactgacttg aatattgacg
    89161 gcagctttat ctcattaggg gataatcgtt ggggcttgcg ttcatggtat gctatcgatt
    89221 cgatcgatga agaagtaaac catggaatgg acgaagagga tgaagatact ccacgtcgcc
    89281 gtaaacgcaa gaaagtcaac gcctttatca atgatgatga agacgcgatc gattacaatg
    89341 acgatgaccc agaagataca gatctgacag aagaagatga cgatgatctg tttgatgacg
    89401 atgatgatga agatgaagaa atcgctgctt ataattctga cttgcaagaa atcggagcag
    89461 atgacccgac agatgatgaa gaagacttgc caggaaatat cgaagaagat ttaagtatca
    89521 tagacgaaga cgatgatgat gaagattatg aagaggaaga attttctgac tctgaaaaag
    89581 aataaacatt attttcttac gagaggttgt gacagagacg atcagcctat cagaataagg
    89641 aggtgtgaca cga
//
LOCUS       DAFCNJ010000005        84916 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000005 DAFCNJ010000000
VERSION     DAFCNJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.005"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1588)
                     /locus_tag="LNE35_000468"
     CDS             complement(<1..1588)
                     /locus_tag="LNE35_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6909653.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
     gene            complement(1603..5124)
                     /gene="mfd"
                     /locus_tag="LNE35_000469"
     CDS             complement(1603..5124)
                     /gene="mfd"
                     /locus_tag="LNE35_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBK6909654.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(5141..5701)
                     /locus_tag="LNE35_000470"
     CDS             complement(5141..5701)
                     /locus_tag="LNE35_000470"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBK6909655.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            6054..7028
                     /locus_tag="LNE35_000471"
     CDS             6054..7028
                     /locus_tag="LNE35_000471"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK6909656.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            7198..8598
                     /locus_tag="LNE35_000472"
     CDS             7198..8598
                     /locus_tag="LNE35_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBK6909657.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(8676..9398)
                     /locus_tag="LNE35_000473"
     CDS             complement(8676..9398)
                     /locus_tag="LNE35_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6909658.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(9477..9983)
                     /locus_tag="LNE35_000474"
     CDS             complement(9477..9983)
                     /locus_tag="LNE35_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK6909659.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            10106..11905
                     /locus_tag="LNE35_000475"
     CDS             10106..11905
                     /locus_tag="LNE35_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBK6909660.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(11973..13547)
                     /locus_tag="LNE35_000476"
     CDS             complement(11973..13547)
                     /locus_tag="LNE35_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK6909661.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(13802..14311)
                     /gene="mreD"
                     /locus_tag="LNE35_000477"
     CDS             complement(13802..14311)
                     /gene="mreD"
                     /locus_tag="LNE35_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBK6909662.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(14315..15169)
                     /gene="mreC"
                     /locus_tag="LNE35_000478"
     CDS             complement(14315..15169)
                     /gene="mreC"
                     /locus_tag="LNE35_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBK6909663.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(15324..15962)
                     /locus_tag="LNE35_000479"
     CDS             complement(15324..15962)
                     /locus_tag="LNE35_000479"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBK6909664.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(15963..16613)
                     /locus_tag="LNE35_000480"
     CDS             complement(15963..16613)
                     /locus_tag="LNE35_000480"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK6909665.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(16626..17525)
                     /gene="rsgA"
                     /locus_tag="LNE35_000481"
     CDS             complement(16626..17525)
                     /gene="rsgA"
                     /locus_tag="LNE35_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBK6909666.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(17690..19759)
                     /gene="pknB"
                     /locus_tag="LNE35_000482"
     CDS             complement(17690..19759)
                     /gene="pknB"
                     /locus_tag="LNE35_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBK6909667.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(19756..20496)
                     /locus_tag="LNE35_000483"
     CDS             complement(19756..20496)
                     /locus_tag="LNE35_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBK6909668.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(20509..21867)
                     /gene="rsmB"
                     /locus_tag="LNE35_000484"
     CDS             complement(20509..21867)
                     /gene="rsmB"
                     /locus_tag="LNE35_000484"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBK6909669.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(21867..22805)
                     /locus_tag="LNE35_000485"
     CDS             complement(21867..22805)
                     /locus_tag="LNE35_000485"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBK6909670.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(22798..23289)
                     /gene="def"
                     /locus_tag="LNE35_000486"
     CDS             complement(22798..23289)
                     /gene="def"
                     /locus_tag="LNE35_000486"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK6909671.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(23304..25712)
                     /gene="priA"
                     /locus_tag="LNE35_000487"
     CDS             complement(23304..25712)
                     /gene="priA"
                     /locus_tag="LNE35_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBK6909672.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(25965..26270)
                     /locus_tag="LNE35_000488"
     CDS             complement(25965..26270)
                     /locus_tag="LNE35_000488"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK6909673.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(26275..26889)
                     /gene="gmk"
                     /locus_tag="LNE35_000489"
     CDS             complement(26275..26889)
                     /gene="gmk"
                     /locus_tag="LNE35_000489"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBK6909674.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(27376..27660)
                     /locus_tag="LNE35_000490"
     CDS             complement(27376..27660)
                     /locus_tag="LNE35_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909675.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(27678..28568)
                     /locus_tag="LNE35_000491"
     CDS             complement(27678..28568)
                     /locus_tag="LNE35_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBK6909676.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(28588..28806)
                     /locus_tag="LNE35_000492"
     CDS             complement(28588..28806)
                     /locus_tag="LNE35_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBK6909677.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(28776..30476)
                     /locus_tag="LNE35_000493"
     CDS             complement(28776..30476)
                     /locus_tag="LNE35_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBK6909678.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            30743..31516
                     /locus_tag="LNE35_000494"
     CDS             30743..31516
                     /locus_tag="LNE35_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909679.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(31597..32166)
                     /gene="thiT"
                     /locus_tag="LNE35_000495"
     CDS             complement(31597..32166)
                     /gene="thiT"
                     /locus_tag="LNE35_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBK6909680.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(32232..32334)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(32340..33311)
                     /locus_tag="LNE35_000496"
     CDS             complement(32340..33311)
                     /locus_tag="LNE35_000496"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK6909681.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(33405..34778)
                     /gene="glmU"
                     /locus_tag="LNE35_000497"
     CDS             complement(33405..34778)
                     /gene="glmU"
                     /locus_tag="LNE35_000497"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBK6909682.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(35281..36099)
                     /gene="purR"
                     /locus_tag="LNE35_000498"
     CDS             complement(35281..36099)
                     /gene="purR"
                     /locus_tag="LNE35_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBK6909683.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(36266..37090)
                     /locus_tag="LNE35_000499"
     CDS             complement(36266..37090)
                     /locus_tag="LNE35_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK6909684.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(37063..37743)
                     /locus_tag="LNE35_000500"
     CDS             complement(37063..37743)
                     /locus_tag="LNE35_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK6909685.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(37766..38704)
                     /locus_tag="LNE35_000501"
     CDS             complement(37766..38704)
                     /locus_tag="LNE35_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBK6909686.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(38949..39812)
                     /locus_tag="LNE35_000502"
     CDS             complement(38949..39812)
                     /locus_tag="LNE35_000502"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBK6909687.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(39954..42527)
                     /gene="mprF"
                     /locus_tag="LNE35_000503"
     CDS             complement(39954..42527)
                     /gene="mprF"
                     /locus_tag="LNE35_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK6909688.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            42668..43474
                     /gene="proC"
                     /locus_tag="LNE35_000504"
     CDS             42668..43474
                     /gene="proC"
                     /locus_tag="LNE35_000504"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBK6909689.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(43553..45109)
                     /gene="rny"
                     /locus_tag="LNE35_000505"
     CDS             complement(43553..45109)
                     /gene="rny"
                     /locus_tag="LNE35_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBK6909690.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(45520..46569)
                     /gene="recA"
                     /locus_tag="LNE35_000506"
     CDS             complement(45520..46569)
                     /gene="recA"
                     /locus_tag="LNE35_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBK6909691.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(46678..47919)
                     /locus_tag="LNE35_000507"
     CDS             complement(46678..47919)
                     /locus_tag="LNE35_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBK6909692.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(48159..48737)
                     /gene="pgsA"
                     /locus_tag="LNE35_000508"
     CDS             complement(48159..48737)
                     /gene="pgsA"
                     /locus_tag="LNE35_000508"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBK6909693.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(48946..49869)
                     /locus_tag="LNE35_000509"
     CDS             complement(48946..49869)
                     /locus_tag="LNE35_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909694.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(50608..51894)
                     /locus_tag="LNE35_000510"
     CDS             complement(50608..51894)
                     /locus_tag="LNE35_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK6909695.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(51887..53155)
                     /locus_tag="LNE35_000511"
     CDS             complement(51887..53155)
                     /locus_tag="LNE35_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK6909696.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            53407..53868
                     /gene="mscL"
                     /locus_tag="LNE35_000512"
     CDS             53407..53868
                     /gene="mscL"
                     /locus_tag="LNE35_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBK6909697.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(53928..54374)
                     /locus_tag="LNE35_000513"
     CDS             complement(53928..54374)
                     /locus_tag="LNE35_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6909698.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(54467..54712)
                     /locus_tag="LNE35_000514"
     CDS             complement(54467..54712)
                     /locus_tag="LNE35_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBK6909699.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(54827..55405)
                     /locus_tag="LNE35_000515"
     CDS             complement(54827..55405)
                     /locus_tag="LNE35_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6909700.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(55482..56012)
                     /locus_tag="LNE35_000516"
     CDS             complement(55482..56012)
                     /locus_tag="LNE35_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBK6909701.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(56016..56876)
                     /gene="rlmB"
                     /locus_tag="LNE35_000517"
     CDS             complement(56016..56876)
                     /gene="rlmB"
                     /locus_tag="LNE35_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBK6909702.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(56869..57264)
                     /locus_tag="LNE35_000518"
     CDS             complement(56869..57264)
                     /locus_tag="LNE35_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBK6909703.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(57261..58673)
                     /locus_tag="LNE35_000519"
     CDS             complement(57261..58673)
                     /locus_tag="LNE35_000519"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBK6909704.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(58670..59206)
                     /gene="cysE"
                     /locus_tag="LNE35_000520"
     CDS             complement(58670..59206)
                     /gene="cysE"
                     /locus_tag="LNE35_000520"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBK6909705.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(59292..59531)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(59528..60988)
                     /locus_tag="LNE35_000521"
     CDS             complement(59528..60988)
                     /locus_tag="LNE35_000521"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBK6909706.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(61158..62315)
                     /locus_tag="LNE35_000522"
     CDS             complement(61158..62315)
                     /locus_tag="LNE35_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBK6909707.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(62349..63719)
                     /gene="radA"
                     /locus_tag="LNE35_000523"
     CDS             complement(62349..63719)
                     /gene="radA"
                     /locus_tag="LNE35_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBK6909708.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(63782..64309)
                     /locus_tag="LNE35_000524"
     CDS             complement(63782..64309)
                     /locus_tag="LNE35_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBK6909709.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            64539..65345
                     /gene="proB"
                     /locus_tag="LNE35_000525"
     CDS             64539..65345
                     /gene="proB"
                     /locus_tag="LNE35_000525"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBK6909710.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            65342..66589
                     /locus_tag="LNE35_000526"
     CDS             65342..66589
                     /locus_tag="LNE35_000526"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBK6909711.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            66665..67009
                     /locus_tag="LNE35_000527"
     CDS             66665..67009
                     /locus_tag="LNE35_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909712.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(67126..67452)
                     /locus_tag="LNE35_000528"
     CDS             complement(67126..67452)
                     /locus_tag="LNE35_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909713.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(68067..69269)
                     /locus_tag="LNE35_000529"
     CDS             complement(68067..69269)
                     /locus_tag="LNE35_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6909714.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            69386..69901
                     /locus_tag="LNE35_000530"
     CDS             69386..69901
                     /locus_tag="LNE35_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6909715.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(70100..70546)
                     /locus_tag="LNE35_000531"
     CDS             complement(70100..70546)
                     /locus_tag="LNE35_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK6909716.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(70593..72071)
                     /gene="msr(C)"
                     /locus_tag="LNE35_000532"
     CDS             complement(70593..72071)
                     /gene="msr(C)"
                     /locus_tag="LNE35_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK6909717.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(72425..73054)
                     /locus_tag="LNE35_000533"
     CDS             complement(72425..73054)
                     /locus_tag="LNE35_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK6909718.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(73038..73502)
                     /locus_tag="LNE35_000534"
     CDS             complement(73038..73502)
                     /locus_tag="LNE35_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6909719.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(73989..75257)
                     /locus_tag="LNE35_000535"
     CDS             complement(73989..75257)
                     /locus_tag="LNE35_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK6909720.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(75488..76225)
                     /locus_tag="LNE35_000536"
     CDS             complement(75488..76225)
                     /locus_tag="LNE35_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6909721.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(76292..76861)
                     /locus_tag="LNE35_000537"
     CDS             complement(76292..76861)
                     /locus_tag="LNE35_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBK6909722.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            76989..77861
                     /locus_tag="LNE35_000538"
     CDS             76989..77861
                     /locus_tag="LNE35_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6909723.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            78203..79075
                     /locus_tag="LNE35_000539"
     CDS             78203..79075
                     /locus_tag="LNE35_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6909724.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            79072..81030
                     /locus_tag="LNE35_000540"
     CDS             79072..81030
                     /locus_tag="LNE35_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6909725.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(81362..82213)
                     /locus_tag="LNE35_000541"
     CDS             complement(81362..82213)
                     /locus_tag="LNE35_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6909726.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            82336..82803
                     /locus_tag="LNE35_000542"
     CDS             82336..82803
                     /locus_tag="LNE35_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBK6909727.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(83066..83548)
                     /locus_tag="LNE35_000543"
     CDS             complement(83066..83548)
                     /locus_tag="LNE35_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBK6909728.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(83648..84511)
                     /locus_tag="LNE35_000544"
     CDS             complement(83648..84511)
                     /locus_tag="LNE35_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK6909729.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        23984 a        18210 c        15313 g        27409 t
ORIGIN      
        1 taattttttt atttaatcgc aggattttct taccaaaagg caacattagc cattctcgta
       61 ctgtaaacag acgaagacga atcactgcat aaatgaacac agaagccccg atcccaactc
      121 ctatcacgct gacaaaaaaa gcagtggaac gatgagttac aggaccaaat actatattca
      181 atacaccata atacagcagc agtactactg tcatacaaag aaggctgaac atcaacttcc
      241 gtccaaaatg ccgttctttc caaaaagcat ttagctcttt tgtttcaatt cgaacaaaat
      301 accaaagcgt cacacctaat cctaaaaggg tagacaggct tgcacctgct gttcctaata
      361 aaccggtcag aaaagaagta gtcaatcctt ttaccaatag tccccatcct gcgcctttta
      421 aagaagggcg aaacgagttc ttgctttgag caatactttg ataagcttgg atgactgcag
      481 tgaaagcaat ggaaaaaaca aataatacaa gcgctgcatt tcccgcataa tctttaaaca
      541 acgcataatt gatataaggg agcagcaagg ctaatcccaa agacgcagct aatgctaaag
      601 cggtcgtcaa tctaagatag attttggctg tctgtaaaaa ctgacgatag atccggttgg
      661 tcaaatgacg agtcaacgca ggcaggaatg tcgcacttaa tgcagtggca atcactaaac
      721 ctaattgtac taaaggctga cctcgatcat agaccccttt tgctatcttc gcaccgtatt
      781 ctgacagtcc gtaaacttcc aaagcatttt ttacaaagaa agagtcgatc agctggaaaa
      841 agataagcaa tccactataa atcgaaacca gtccgccttc taccagaaaa cgccggatca
      901 agggctttga cggctgtttg aacaatggaa accgtcggaa gcttaacgct cctccatgaa
      961 tcttttgttc atagtagtga aggatcccat atgcgcataa accgccaaaa acagctccac
     1021 tcattgctgc cgtccctgtt tggtacaccg tccatccaag ctttctaaaa gcaaacgcac
     1081 tacagacaat cacaaaaaca cgaacgaact gctcaactac ttgagaaaca gcagttggtt
     1141 ccatcaagaa tcttccttgg aaattccctc gataaaaaga tagaccaggc atcagcaaaa
     1201 aggtaaagga aactacttgg atcagcggtt tcaattgtat gtctcccatc atccaagcaa
     1261 tccagctgct aaatgcaaag acaaacaacc acaaaaagac acctgtccaa aatactaagg
     1321 gataaagatt ttgcaaagct tgctttcgct cgaaaggatt ctttctttct gcaacgtact
     1381 ttgatatgaa ctgcggaaga ccggataaag ctaaagtcat tgctatccca taaattggat
     1441 aaacttgctg ataaacataa aaaccttcgt cgccgactaa gttttggagg ggaattcggt
     1501 aaagggcact taatactttt gctatgaaag aagcaatcgt caatacaaat gctccttgca
     1561 tgacgcgccg catttcttta tgatccaaat cattgctcct tcctacgctg acagatattt
     1621 ttgctcacgc aacgctttga caaacttttg gatttcctga agccatactg cttcttccat
     1681 ttggttgggg atcgtcaagc gtatcaccat ctgttctttt tcaacgccta gactggcttt
     1741 tagttttgtc gctgtcagtg cttcgaataa ctgttccaca ctatagcgtt ttgttccaac
     1801 tttacttaga gtaaagacta tatgcgcctc ctgtttcctt atcgtttcta ggagcgctcg
     1861 atcgccatcc attttgatct gcccaattgt caagagatgt gctacttctt ctggatattc
     1921 tccaaaacgg tcgagcagat catcttctag ttcatctagc atttcttgcg aatcaagttc
     1981 acgaattcgt ttatagatct cgattttctg ccgttgatct gccacgtatg tttcaggtag
     2041 atacgcatcg acaccgaggt cgatctcaac agtggtcttc tccacttgac tattctttcc
     2101 ttgtttacga gaaacagctt cgcttagcat ttgagaatac atatcgaaac cgactgcatc
     2161 aataaatcca tgctgctgag cacctaagag attacctgca ccgcgaatgg aaagatcccg
     2221 catcgcaatc ttgaatccag atcccagttc tgtaaaatct ttgattgcct gaagacgttt
     2281 ttcactgact tcgttaagga tcttctgctg ctcatacata aagtaagcat atgcgacacg
     2341 gctgcttctt cctacccgcc ctcgcaattg gtataacgtg gataatccca tataatcagc
     2401 gttctcaacg aacaacgtat ttgcgttcgg gatatctact cctgtttcaa tgatggttgt
     2461 agtgacaaga acatcatatt gaccttcaat aaattcaaaa agcgtatttt ctaattgcgc
     2521 ctctgtcatt tgcccatgag cataaccgat ccgtgcttcc ggaacgagtt cttggatttc
     2581 ttctactttt tgttcgatcg tttctacccg attatataaa tagaatactt gtccacctcg
     2641 tcccatttct cgatggattg cttcgcgaat agccccagga tttttttcca taacatacgt
     2701 ttggatcgga taacgattcg caggcggtgt ttcgattact gaaagatccc gcacgcctaa
     2761 catcgacata tgcaaagttc tcggaatcgg tgttgcagtc agagtcaaaa catccacttg
     2821 tgcacgtaac tgtttcaatc gttccttatg ttttacacca aaacgttgtt cttcgtcaat
     2881 gatcagtaga cccaaatcgc taaattcaat atcttttgac aaaattcggt gggttcctac
     2941 aacaatatca acttgacctg tacgcagttg ttcgatggtt tctttttgtt gctttcttgt
     3001 tctgaaacga cttaatagcc cgatatttac ggggaaacct tcaaatcttt ccagcatcgt
     3061 ttcatagtgc tgctgtgcca agatcgttgt tggaactaaa aaggctactt gtttgctttc
     3121 ctttatcgct ttaaaggccg ctcgaagcgc tacttctgtt ttcccataac caacatcacc
     3181 tacgagtagt cgatccattg gtttttcttt ttccatatcc cgtttgatct ccgctgcact
     3241 tctcaactgg tcatcagttt cagagtaggg gaaggcattt tcgaattctt tttggtaggc
     3301 gtcatcaggt ccaaaagcat atcctttttc tgactctctt gcggcatata atttgattag
     3361 atcgtcagcg atatcctcga tttttgaaga gacttttcgc ttggttttcg tccattcact
     3421 tccgcctagc ttattgatgc gaggcgcttt ggcttcagag gcgacatatt tttggatcag
     3481 attcagttga gtaacaggaa taaacagttt gtcgtcgttt tgatacaaaa tagtcatata
     3541 gtcttggtga acgccatcta cttctagcgt ttccatccca atgtatttcc cgataccatg
     3601 attggcatgg acaacgtaat ctcctgcttt taactcatta tagcttttca atcgctctgc
     3661 gtttgttact gtttgacgtc tcgtttgttt tttcgtcgtt ttttggaaaa tttctttttc
     3721 tgtgatagct actatttttt cttgaggaag ttcaaaacca gcctgcaaag ctccttcaac
     3781 taattgtacc tgtcctctga tcaatgcatt tttttctgtt tctactacta atatgtcttc
     3841 atctcgaagc atttgttctg ctttgcgtat tctgtccttt gtcggaataa agatcacaac
     3901 agtctgtttt tgcttgcgcc aacggtccat ttctgttttc agcaatggca tttgtccgaa
     3961 gaactgttgc atcgtccgat attgaaaatt atggattgct tggaaacgaa gatttcccat
     4021 ccctttttgg aagagtgaga aaaaggttgt ggtgaacttt tccttctgca cgattttttg
     4081 tatatctgct gaaaatgtct gctcagaaaa cacccgcaat tcgctgattt tttgagtctg
     4141 ccattctgcc gcttcccttt cgatttcccg atttgtttcc atgattcgct gataatcatc
     4201 aacgactagt aaactatttg aagggaaata atccaagatc gtcacagatt cttggtaaag
     4261 aagatctgag tagaattttg cttcttctgt cgggacacct gcttgccagc ttgaagtcag
     4321 ctgaccgaag taatcttgca aaaactcgcg gtctgcttct tctgtggtcg cagcaagccg
     4381 tttttctagc attttttgca tttgctctat tccatgttct agatcctctg gtccgtagac
     4441 ttgttcactc gttggggaaa cccagatttc ttccgttttt tcgattgagc gttgtgtttc
     4501 tgcttcaaaa taccgcatag aatctatctc tacatcaaat agttctacac gaacaggata
     4561 ggttgtcgtt aacgggtaaa tatcgataat gcttcctcga atactgaatt ctcccggttt
     4621 tgcgaccatc tcttctcgta catagcccat caaagtcaat tggcgaggaa gattttccac
     4681 gtctatctca tcaccaatat gccaattcaa ttgcgcctgt agccaagttt tcttattcgg
     4741 cagtcgtttg tgcaaagatg cgactggcaa caaatagata cctgcttttt tttcttgaag
     4801 tgcgttaagc gttgcaactc tctcgcctcg cgcttctgga gaagaaaagg ccatttcagc
     4861 agaaagcact tcatccactg gaaataaatg gatctgatct tgcggaatca catgttgcag
     4921 atcttctgct aactgattcg cataatacaa atttggcatc agaataatca tgtttttatt
     4981 tgttttctta aacgctcgag caaatagaag tgttttcgct gagcccgcta atcctgtgat
     5041 tagttgtcta ggcatttttt cttctaaatt gctgacccat tcttttactt gcggatcttt
     5101 tccgactaat tgaataatat ccattccttt tcctccttcc tcaattgaat cgattcatcg
     5161 catccacgaa cgaatgccct tctatgagat attcgactgc tttaacagat tgacacatgc
     5221 tttcttctat caaaggctga ttttctttgc tgaaaggact aagcacatgc tggactactg
     5281 tttttttccc ttctggcctg ccgattccca ccttgattcg atcaaagaca ttcgtattca
     5341 aatgggaaat aatacttttg ataccgttat gcccgccggc acttcccttt tgacggagcc
     5401 ggatttttcc cactgccaga tccaaatcat cgtagattac gactaattcc tctggataaa
     5461 tcccaaaata agtcattagt gggccgactg cacgaccaga ttcattcatg aatgtttgtg
     5521 gtttgactaa aaggatcttt tctccattat gaaaaaattc ggcaacttct gcttcgaacg
     5581 gatttttttt gaaagtagct tgatgctcgc gtgcaatttt atccacgacc ataaagccga
     5641 tattgtgttt tgtatattgg tatttggtac ctgggtttcc taacccgacg atcattttca
     5701 tttttgtttt ccacctaatt ctaaaaagca gcacaaaagg ctggactgct aattagcggt
     5761 ccagtccatc gagctttttt cttttttaca cttgtcaatg catagtataa cacaaaacaa
     5821 ggcgtttcgg ctaaaagaga atcgttaata aatgataaag aaattaaaaa tttcttcaaa
     5881 gtttaatgaa ataattgata tataaaaata gtgaaataac gcacatgtaa gcttttacat
     5941 ataatacaac cttgttatct gttatatttt atagccaaat agcgtgacat ggggcttgag
     6001 aaatgttagc ataagcatgg gaaaaaacaa atcaatggaa ggattgatac taaatgactg
     6061 cagctgcaga aaaaaaagat catcaaaaag ttattcttgt tggagacggc gccgtaggtt
     6121 ctagctatgc ctttgctcta gttactcaaa atatcgctca agaagtaggt attgtggata
     6181 tcaatactgc taaaactgaa ggagacgcaa ttgacttgtc tcacgctttg gcatttactt
     6241 cacctaaaaa aatctattct gctacttacg cagacgcaca tgatgctgac ttagttgtta
     6301 ttacagctgg cgcaccacaa aaaccaggtg aaactcgttt agacttagta aacaaaaacc
     6361 taaaaatcaa ccgcgatgtt gtcacacaaa tcgttgaatc aggatttaac ggtatctttt
     6421 tagttgccgc taacccagta gatattttga cttactcaac ttggaaattc tcaggtttcc
     6481 ctaaagaacg cgtaatcggt tcaggtacct ctcttgactc agctcgtttc cgtcaagcac
     6541 ttgcggaatt agttgacgtt gatgcacgta acgtccatgc ttacatctta ggtgaacacg
     6601 gagattcaga attcccagtt tggtcacatg caaatgttgc cggcttgcaa atctatgaat
     6661 gggtaaaaaa caacccagaa atcgatgaag aagctatggt taacctattc tttggcgtac
     6721 gtgacgctgc ttacacaatt atcgagaaaa aaggcgctac tttctatggt atcgcagtag
     6781 cattagcacg tatcactcgt gccatcttag atgatgaaaa tgctgttctt cctttatcag
     6841 tattcatgaa tggcgaatat ggattgaacg acatctacat tggtgcacct gctgttatca
     6901 accgtcaagg tatccaaaaa gtaatcgaaa ttcctttaag tgacagcgag caagaccgta
     6961 tggctgcttc tgctaagcaa ttaaaagaaa ttctagatga agcttttgct aaactagacg
     7021 ctgaataatc tttgaacatg acggaaaaca tctattcggt gttttccgtt tttttatttt
     7081 atcaccctta tggtttctaa attttttcat aaagtagaga taaccttcgt cttttcctct
     7141 aaattatgct atcatgagac cgttccaccc taaactttta cttaaggagt tgtcgatatg
     7201 acaagatctt cgcatcgaaa aaaatcgaaa catgttgctt tgctttcagt tcttggtgtt
     7261 ctcttagtgc tgatcagctt ttatacctat cgcagtacat actacacaaa gcattttttg
     7321 ccgaatacag aaataaatgg aataaatgtc agcaatctaa cagtcgaaaa agcaaatgaa
     7381 aaattaaaag aagcttattc tgataaaaaa ctggctatta aagaagatgg aaaagtctgg
     7441 caggaagtac caaaatccga gctcggctac aaagatgatt tcacttcgga gctttctccg
     7501 atcctaaatg aacaaaacgg atggacttgg ggaatgactt atgtctcagc ggcagaaaaa
     7561 caagaaatag atcctgtcag tcaaaaccac caaaaactag acactaccgt tcagacattg
     7621 acaactaagc tgactgattt gaacaaagac cggacaccta ctactgatgc aacgatcgaa
     7681 aaagcaggtg actctttcgt tatcaagcct gaagtcaatg gaaatacgat cgacgtcgat
     7741 gctgcggtca aacaattaaa aagtgcggta aactctggta aagatacaat tgaattgact
     7801 gaattcaaag aaaaaccaaa agtaacatct gaagacagct cactgaaaga acaactcgca
     7861 tccatgaatg ctatcgcaaa tgtaaaagct acttacagta taaacggtga gactttccag
     7921 atcccatcaa gtgatattat gagttggctg acctacaatg atggaaaagt cgatttagat
     7981 acagaacaag tacgtcaata tgtaacagat ttaggcacaa agtataacac aagtacgaat
     8041 gacacaaaat ttaaaagtac aaaaagagga gaagttacgg tcccagttgg cacttacagt
     8101 tggacgatcc aaacagacag tgagacagaa gcattgaaaa aagcgattct ggcaggccaa
     8161 gattttactc gttcacccat tgtgcaaggt ggcacaaccg ctgatcatcc attgatcgaa
     8221 gatacgtata tcgaagttga tctggagaac cagcatatgt ggtattacaa agacggaaaa
     8281 gtggcccttg aaacagatat cgtttccgga aagccaacta cacctacacc tgctggagtc
     8341 ttctatgttt ggaataaaga agaagatgca actttaaaag gaacaaatga cgatggaaca
     8401 ccttacgaga gtccggtaaa ctactggatg ccgattgact ggacaggtgt tggtatccac
     8461 gattcagact ggcaacctga atacggtgga gacttatgga aaacccgcgg ttcacacggc
     8521 tgcatcaaca ccccaccaag tgtaatgaag gaattattcg ggatggtcga aaaaggaacg
     8581 cctgtattgg tcttctaaca aaaataatat ataaaaagaa agcacccgta cgattggtac
     8641 aggtgctttc tttttattat ctaacaatgt gctacttaca tttcagcagc agtaaagact
     8701 tctgaaacat catcatcgtc ttctagctta tctactagac gttccaattg ctctttttgt
     8761 tcctcattta aatcaatcgt cgtttgtgga atcatcgtca attctgcttg cgccaaagtg
     8821 aactcttttt ccaactcatc tcgtacagca gcaaaatctt ctggtgcagt ataaatctcg
     8881 aatacttcag gagaagtttg cagatcttct gcccccgcct ctaatacatc ctcaaacatc
     8941 gtctcttcat caacagtcaa cccatcgcgt tcaatagcga tatatccttt gcggtcaaac
     9001 aagtaactga ctgaacccgt ttctcccagt gatccaccgt ttcgagtaaa agctactcgg
     9061 acatttgtcg ctgtccgatt acggttatct gtcaatgcat ggactaagat tcctactcca
     9121 cccggaccat atccttcata cgtgatttca tcgtaattcg tttcatctac ggcactcgta
     9181 gcctttttaa tcgcccgttc cacattgtcg ttaggcatat ttgcagcttt tgctttgtcc
     9241 acaacaagcc gcaaagctgg attcatagat ggatcaggac cgcctgcttt agctgccata
     9301 taaatttcac gagaaagttt ctgaaagact ttccctcgtt ttgcatcttg ggcatttttt
     9361 cttccttgga tattatgcca tttactatgt cctgacatga tttttcctcc cttgttcttt
     9421 tgagaataca cttctattct agtactattt cacaaaaaaa caaagccaca gccaatctat
     9481 aaacgcttct tgaatgccgc aaaaagcaca cataatatca cagcagtcag agcaccgatc
     9541 gaatccaaca tgacatcttg gaacataggt gttcgtccac cggtcatcat ctgatggaat
     9601 tcgtccaaac ctgcgtaccc tgttgctgaa agccaagcaa ggatcgctgt cagccaccaa
     9661 atttttagct tggggaaaac acctaaaaac aagctgcctc ccaaaataaa ataggtaccg
     9721 aaatgtgcgc ccttccttaa aaagaactct aaaaatttgg cgtagcccag atggttgata
     9781 ctcaccatac ttccaccata attaaaacgt atacccgcta atgtgtcttt aaatggctga
     9841 ttaggcagcc agctttctaa tcgcgaaact tgtgattgct gtccgtaagt ttgagaagaa
     9901 ctgtaaaata agattataac gactatcagt gcgattacta aataaatatt tccgtttttc
     9961 atttgttttt ttgaagaatt cataagtcac ctctgtattt tatcttagac tttttttgaa
    10021 gaaacaacaa tcattttcat agagacttct tccataaaaa agtgctatac ttaattttgt
    10081 attacaatat gaagggagtt ttttcatgag ttattcatta aattggaatc tagattctat
    10141 tttttccgga ggcagtcatt ctgatgcttt gaaccaacgg atgaagcaat tggaagacca
    10201 aatgaacgag tattatcacc gcgtaacaaa atggtcacct agttctgaca atgcagagca
    10261 gttaaacgct attcttcaat tgcaagaaac gattacgaat ggttttaccc aatgcagcag
    10321 ctatattact gcattgcttt ctgccaatgt caatgattca gatgcaaagg tcttatcagg
    10381 caagctttac gcaatgctgc ctcgtctgca atcagccgaa actgtattgt caaagaaatt
    10441 tgcagaaata tctgacaatg attggaatca gatgctttct caatcttcgt ttgaaacaat
    10501 tgcttttcgt ttaaacgaga ttcgtcgtga tggctcacaa ttattatcag aagctgaaga
    10561 aaacattata aatacgcttt ctttagatgg tctaaatgct tggagcagtc attatgatac
    10621 gattgtagcc agcatttcga ttcccttcga acaagatgga caagtagtcg aattatctgc
    10681 tggacaagcc ttcaataaga tgatgggcga tccagatcca aaagtcagag aaacactttt
    10741 cgcagcatgg gaagaagcct ggaaagaaaa ggcacccctc ttcacggata ctttgaatca
    10801 tttagatggt tttagacttt ctgactatga acttcatggt gtcacagatt ttctacaaaa
    10861 accattagag tacaatcggt tgaagaaaga aacattgtct gtcatgtggg atacaatcca
    10921 gaaaaacaaa caaccccttg ttaattactt gactagaaaa gctaatcttt tcggtaaaga
    10981 aaaaatggaa tggcaagatc aagatgcacc tattatttta ggcgatttga aagaaaaaac
    11041 ctttagtttt gacgaagcag cagcatttat tatcgaaaac tttaacaaat tcagtccaaa
    11101 aatggctaaa tttgcccaat ctgcttttga aaagagctgg atcgaagcag aagatcgtcc
    11161 tgggaaacgt cctggtggtt attgtactga gttaccagaa acaaaagaat caaggatctt
    11221 tatgacctac agtaattctg ttaatgaggt tgcgacactt gctcatgaac taggacatgc
    11281 tttccatagc agcacgatgt gggatttacc ttctttaaat cgcgaatatg ccatgaatgt
    11341 cgcagaaaca gcaagtacat tcgcagaact gatcgtagcc gatgctacat taaaagtcgc
    11401 aaaaacaaaa gaagaaaaaa tcaatttgct agatacaaaa ctgcaaaatg ctttagctat
    11461 gttcatgaat atccattccc gattcatttt tgaaaatcga ttctatgaag cacggcaaga
    11521 aggattagtc tcagaagata agatcacaga gatgatggtt gaggcgcaaa aagaaggcta
    11581 tcaaggagct ttagctactt accatccata tttctgggct gcaaaattgc atttctttat
    11641 tgatgacgta ccgttttata acttcccata cacatttggc tacctcttta gtttgggcat
    11701 ctatgcttac gccaaccaaa agggagcaaa ttttgaagaa gaatatatcc atttactacg
    11761 cgatactgcc tcaatgacga cggaagatct tgcgaagaaa catttgaatg tcgatcttac
    11821 ccaaccagat ttttggcaag caggtatcga catggtgact gaagatatcc aaacgttcat
    11881 ggaattgaca gaagaattta tctgatttct attttatcac aataaaaaaa gagttagtcc
    11941 agaatgggct aactcttttt gattaatcat tcttacatgc tgacagcaaa gtcaggggca
    12001 aaccattgaa ctgaaccaac ttttacgctt tcgcctggtt gaggagcatg gatatattgt
    12061 cctccgccta aagcaatcgc tacgtggtaa gttccgcctt gtgaccccca gaataacaag
    12121 tctccagctt ttgcttgaga aactgaaatt tttgttccag ctgattcttg aggaactgtc
    12181 caaccaccga tgtcacgacc agttacttgc atgtaaacat agcgtgtgaa ccctgagcag
    12241 tcaaagccac ttggatcttt accgccccaa acatatggag taccaatata tttgtaagct
    12301 tctgctacga ttgctgcacc attcacactt ccagaaggag ctggagcagg tgttacaggt
    12361 tttggttgtt caggtgttgg tgttggcgtt ggagccggag tactacttcc tgtgccgttt
    12421 cctgtatcaa cactttgatc tgttgatggt gttgtaggcg caggtgttgt tgattcttct
    12481 gttgttgaag attcaggcac tgttgttgat tcttctgttg ctgaagattc aggcactgtt
    12541 gttgattctt ctgttgctga agattcaggc gctgttgttg attcttctgt tgctgaagat
    12601 tcaggcgctg ttgattcttc tgttgaagat tcaggtgttg ttgattcttc tgttgctgaa
    12661 gattgtgtag ctgatgatga ttcttcagtc attgaagatt gtgtagctga tgattcttct
    12721 gttgctgagc tttgtgcagt agatgaagct tgagtagctt gtgcttcagc ctctgcttgt
    12781 tcgcgtgctt ctttttcagc tttttcttgt gcagcttgtt gacgagcttg ttctgctaga
    12841 cgttgttgtt cacgaatacg agcttgctct gcttcagctt ctgctttttg acggttcaag
    12901 tcagctttct tgtcttcagc agttgcttgt tcagcagcta atgaagtttt cagtacgttc
    12961 aaatcagctt gtttagataa aagatcgcct ttttgagatt ccaaagcagc ttggttttca
    13021 gctaattctt ttagtttagc atcgttttca gcttttttgt cttctacagc ttttttatct
    13081 tgtttttgtt gttccatcaa gtcgttgttt gcttttacca tcgttgtcat tgcttgaacg
    13141 cgtccaatag catctgctaa tgaatcagca ttcaatacag catcgatgta gtttgagctt
    13201 gtgttgctta cttgtgcttc gcgtgcttgt ttttggattg tatcttcacg tttttcaata
    13261 cgttcttgta aatctgcaat gtcttttact aattgtgcag attcttgacg caatgtatct
    13321 tgttttgcta ataggtcttg tgcttgtgtg ttgatttcag aaacttggct ttctaaggca
    13381 tcgatttgtg attgtgcatc tgcttgttga tttttcaagt ctgcaatctt ttgatcttgc
    13441 tgttgtatct gagaatcaaa atcgtccgcc gcggcagcga tcggtgatgc tacggcagtc
    13501 aaggtcattg aacataccat tactgctgat attaaactct ttttcactct tcattcctcc
    13561 gactggctta atttaataaa tatctaaata tatttaagtc tttcttaacg atataaaaat
    13621 tgtaccatag cagtgtgtca acgatataat agttatgtta caaaaatgtt tcaaaacaac
    13681 ttttaaaaat gacaaaactg ttcgtttcag aaaatgatca tgaaaaagcc gcgttaagtc
    13741 tagcatttct taagataaca acgattcttt ttcgctttcg acctaaaaat aaaaaaagaa
    13801 attattcatc actgaataat ttcttaaagg gaaaaatgaa taatacaaat ataatcatgt
    13861 tcaacagcag tgtaggtcct aaaaaccttg taataaaata cgtgttattt actactgcta
    13921 atttaaaaat caactggacc accatcgtaa acaattcata acccgtgaca aagatgatca
    13981 tactgaaaaa ttcagtaaag atgttgacat ggataacgtc tttcattaca tacatcaacc
    14041 atacaattaa agggagagct actgcataga taccgataac gccaatatag taagcgtcat
    14101 agatagcccc gagaacaatc gtggtaatca gtaaataacg tttttcaaac gccatactgc
    14161 aacataataa tgccaatatc agaaagtggg catttgacat ataggtgcct ttcgaccact
    14221 cccctagcat tcttgttaaa tgaccatcta tcaacatcaa aaagaacaaa acgattggca
    14281 gatagtactt cattgtttct ttcttcaaca tggactaatc ctccgctagc cgttggacaa
    14341 tcgtcacaac tgacacgtca tacatttctg cataaggttt cacataaact tcccgatcca
    14401 gcccataact atctggtttc gttttaataa ccgtaccgat cggcaagtta gctggagaat
    14461 tccctccaag accggatgtc tggacaacat ccccttcttt gatatccgta tcaccagtta
    14521 attgggtcac cactaaggca tggagctttt catcatagtt tttaagcaaa ccaaacgctt
    14581 cgccattagc cgaagatacc cgtactggaa aatgattgga gctttcatta gaggatgaca
    14641 gtaattcgat tttagacgaa gccgtattga cctcgatcac tcggccaatc aatccttttt
    14701 gcgacatgac tgccatattt gcttcgatac cgtctttaga acccttgtct acgatcagca
    14761 tgtcctgcca actgtcaggc gaacgagtaa tgacatttgc cgttactttt tcgtaactag
    14821 tcagcgtctg attcaactca agttcttttt tcaacgcttc gatttctctc tcgtaattct
    14881 tgttttgctg tgaaacgtca tcatagctat cgattttttc ttttagccgc tcattttcgg
    14941 cataagttga aaaaagatta tggacagaat ctactccatt tcgtacccac cgtactgggg
    15001 cagagattgt tttatcaacc attgcaacac tgtcattaac gatggattgg aagaaattgg
    15061 gctttccttc attcgctcgc tgcgcagcgg tcacacttag tatcgtcacc acgatgataa
    15121 caataaccaa ggtaataata atatttttat taggattgaa cttcttcaca aagacacctc
    15181 gaattaatta gactcttgtg ttcaatttta tcactaaatg ccgtgaaaca aaaggtaagt
    15241 tgctcctctt aagctttttt ttagcaaata atagattctg tcatttttag cctgaaagac
    15301 aaattttttt atttgtttat ggatcaatct ttgctttgaa taactgcaat cataccatct
    15361 gaaaaagaaa attctgcggt atctgaaaga aattcgttac ttgcataaga tgtcggatat
    15421 tctacttctt tattttccaa tacatacttg ctgcctttta atgtaagatt cgagacggga
    15481 gtcaagcagc aataagctaa atacttcata tctggtatct gtcttactat atgctctcca
    15541 ggcaaataat aagacaaata attttgacaa tctctgataa taagctgatg ggcaaagggt
    15601 ttgaagcgag gttccatacc taaccagaga ttggctaaaa gatgatccaa tcttccgccc
    15661 gtagctccaa tcaaagtgac ttccgcttgc gggtaacgtt caaatgtttt taagatacct
    15721 aactgtgtat ctgtatcgtc tttttctgca ggggaacggg tatactcttt ggcttttgca
    15781 aatacttcct tctgctcttc gttcgacaat gaatcaaaat cacccactgc cagatctact
    15841 gggtagcctg cttgaagcag gtacagcgca ccacggtcta tcccgacata ggcatcgtat
    15901 ttggacagca gagactgcgg ccagttttgc ggattacctc cagcagctaa taaaattttc
    15961 atttagtcta atgcctcttt taatcgttcg atttgtccga ctggattttc ggctccatag
    16021 atatatgatc ctgcaacgaa gacatccgcc cctgcatctt tacagatttt tgctgtttct
    16081 ggaacgattc cgccatctac ttcgattgca taagtccaat tgttctctct acgtagatca
    16141 gcgagttccg taattttttc tactgtttct tcaataaagc tttgtccgcc gaacccagga
    16201 ttgacagtca tgactagtac ttgatcagcc aaaggcaaaa catgtttgat catggagact
    16261 ggtgttcctg gattgataac tactccagct ttaactccta agctttttgt catttgaagt
    16321 gcccgatgaa tatgaggagt tgcttcgacg tggacagtaa tgatatctgc tccagctttt
    16381 gcaaatgctg gaatgtagtt ttctggatca acgatcatca gatggacgtc taacggtaat
    16441 ttagtaacag gacggattgc ctgaacaata tttggcccta atgtgatatt tgggacaaat
    16501 cggccatcca ttacatctac gtggatgtaa tctgcgcctc ctttttccac taactcaatg
    16561 tctctttgta aattggcaaa atctgcactc aaaatggatg gtgctatctt cattcttgtt
    16621 tcctcctatt ttcgtttacc ttcttttttt gcatacattg gttttctttt ttcgatttct
    16681 tgtaagaatt gcaaataatt gtcgtaacgt gtctgtgcga taccacctgt ttctactcta
    16741 tgttttactt cgcagccagg ttctttagca tgcatgcact ctctaaattt gcaatggaca
    16801 gaagcttcta caaattcagg aaattgtctc ggcagttctc ctgcttcaat tgtcaaaaag
    16861 tcaatcgagc taaatcctgg tgtatctgct actaacccgc catagatagg aatcagttcc
    16921 acatgacgtg ttgtatgccg tcctcttcca agtgcatcag aaatctctcc tgttgctaaa
    16981 ttcagttccg gtgatatttg attcagtagc gtcgattttc ctgctccaga ctgacccata
    17041 aagaccgata gccgttctgg gaagtaacgg atcagttctg tcgtatcacc cgtatgattg
    17101 ggtacaatca ccggatagcc tattttagta taggtttgct tgatctcttc gattttttct
    17161 gggtcgtctg acaagtcggt ctttgtcaaa aaaataatcg gttcgatcga ctcgtattcc
    17221 aaggtaacta gaaagcggtc aagtagatta taagaaaatg caggctctac gatacttgtg
    17281 acaatgattc caagatcaac atttgtgact ggtgggcgaa ctaattgatt ttttctcggc
    17341 aagatttcca gaagatagcc gtctgtttga ttttcactct caaagatcac ctgatctccg
    17401 acaagcggcg tgattttccg attccggaaa tttcctcttc ctcttgtttg ataggtctct
    17461 ccgtttgcgt atacatagta aaaaccgctg attgcttttc tgatttgtcc tttgataagt
    17521 gccacctccg tttttgttta ttttaccata cgaagaggaa agtaggtgtt attccatgca
    17581 aacttctcaa agtatatggc ttgaaaattg atcaaaagag atagaaacac aaaaaagaga
    17641 atgcaacaaa tcaatgtcgc attcccttct gatatatttt attcagcact tattttgaac
    17701 tttctgaggt gctagctgat gaacttgtgg agctggaaga ttcttctttt gaagagttgc
    17761 ttgtgctact ggagctactt gagctgctag aactgtctga agagtctttt tcagaactag
    17821 aagacgctgg atctggtcct ttagagtaaa ttacgttcac aaccattcct ttggtaattg
    17881 tcgttcctgc ccctggtact gtacgaatca ctttatcttt atccaccgtg tttgaatatt
    17941 cggaagtctc atgcccgata tattctgcac cgacactagc taaataactt tgtgcttcgc
    18001 tttttgtata gccggagata tcattcaatt taggctctgt tccttggctt acgacaaaat
    18061 tgatgtaagt ttcttctgct acaacttcac ccgaggctgg atcctgactg atgatcgaac
    18121 ctgatggtac agtgtcgcta tagtcataag tagcactaat attattgata ttgaatcctt
    18181 gtgtaacaag ccaattacga acagtttcgt agttttctcc tacatgacta ctcaaagtca
    18241 ctgtttctgg tcctttgctg actgtgaaag taatcgtgtc tgttttagga tcgacttctg
    18301 tatcttctga tggagattgc gaaatgatca ttccgctttc tacctctgaa ctgaattcct
    18361 cttctttttg aatcaagtca gcagaaaacc ctaattcttt caaacttgct tcggcatctt
    18421 cataactttt accactgtaa tcatctaatt tgattttttt cgtgccgcta ctaatataca
    18481 gagtgacttc gcgtttttgc ttgactgtcg ttcccgctgc cgggtctgtc ttcactacat
    18541 taccttcttc gattttgtca tctgcaattt cttctgtttt tgttgctact tctaatttcg
    18601 cttttgcgag tgtttctcta gcagaagctt cagaaagatc agaaacatca ggaacaacaa
    18661 cttctcctcg cccgccagaa gcaaagaata cagctgttcc gattcctaaa acagcaagaa
    18721 tagctaatag gatcagccac cattttttct gtcctttctt tttaggatca ggcagaactt
    18781 cttcctcctc tggtaaggtt tcgttttctg gcttgtcttt tggcagaggc atagatttga
    18841 atgaatcagg cattggtgca tcttccgtaa ggggtgtgat aacttttgtt tcattatcca
    18901 tcacatgagg ctgccatttt tcttcgtttg cacgagctgg tgataatact gtagaaagat
    18961 catcagacat ttcctcggct gacttataac ggtctgcagg ttctttagcc gttgcccgta
    19021 aaatcacgtt ttccagtgct tgtggcacat ttggatcaag tgcttttata gatggaagat
    19081 catcttgaaa atgttttaaa gcaattgtta cagcagattc accatcaaac ggtacgctac
    19141 ccgtcaacat ctcataaagg atgatcccta aagcataaat atctgactgc ttcgttgcca
    19201 tacttcctct ggcctgctca ggggacagat aatgaacaga tcctagcatc gtattcgtct
    19261 gcgtgatcga tgtttcagat aaagcgatag caatcccgaa atccgtgatt tttacaacac
    19321 cttcattatc gatcagtacg ttttggggtt tcaaatctcg gtggatgatt tgatggttat
    19381 gagctaaaga aatagcagaa aggatctgct gcataatatt tactgctgtt tcataaggaa
    19441 caggataatg tgtttggata tatcgtttca agtccattcc tttgacgtat tccatcacca
    19501 gatactgcat gttgtcttct tcaccaacat cgtaaacact aacaatgttt ggatgaacga
    19561 gttctgttgc tgctaaagct tctctctgaa atcgtcgaat cgcagtctgg tcattttgaa
    19621 agtcaaatcg taagacttta actgctacat cacgatccaa gattaaatcg tgcgccaaaa
    19681 atacatttgc cataccaccg ctaccgatat ttccagtgat ttgataacgg ccatttaatt
    19741 ttttgcccaa ctcgatcata cactgtcctc cttatattcg ataagaagaa cggtgatatt
    19801 atccgctccg cctgcttcat tggcacgatc aatcagctca gttactttat gagacaatgg
    19861 gccttcttga ttgatgatcg ttcctatttc ttcttcagac agcatgttcg tcaagccatc
    19921 tgaacataac agcagtcgat cttttagttg ccaaagatga gtagaaacat ctacttcgac
    19981 tgttccaggc atgccgacag aacgtgttaa aatatttttc cttggatggt tcactgccat
    20041 ttcttcgctg atttctcctg atttgactag ttcattgact aaagaatgat cctccgtcaa
    20101 ttgcatgatt ttatcttcac gaatcaaata agctctgcta tccccaacat tggcaatcgt
    20161 gaaccagtca tctgacagag cggccgctac gattgttgtt cccataccgt tatattccgg
    20221 ctgttcttgt ccacgctgat aaatcagcat attttcttct tgaatcgatt gaatgaacca
    20281 ttgggcgatt ttttcatcgt caacagcttc ttgtttttcc caagcttcgc ctagattcgt
    20341 taccgccatc tgactggcaa tgtctccggc acgatgacca cccatcccgt ctgctaatac
    20401 agcaagcttg agacctgaac ggctggtaaa tacactggca tagtcttgat ttgtgttacg
    20461 tcgtcttcct acatctgatt gatattcaat ctgcattttt tcacctcatt actttttgcg
    20521 caaacaacaa atgaaaaatc catctgtgta aaactgatgc ggatacaaag tcaacatttt
    20581 ctcttcgatt gatgattgta gcataggatt ggtcaaaaca tcaatcctct caaaatcagg
    20641 gtgtctttcc aagaaagcag aaacaacgtc ttggttttct tcttttaaga tcgtacaagt
    20701 gctataagtc agaataccgg aagactttaa agttggtgcg cagctttcta aaatttccaa
    20761 ttggattttt ggtaaattcg ctaattcatc cggctgtttt ttataacgaa tatctggttt
    20821 tcgccgaagc agcccaagac ctgaacaagg cgcgtctact aaaatccgat caaactgctc
    20881 tgcttcgaac tgtttactga cttctctagc atccatcttt tgagcgtaga cagcagaaga
    20941 gacacctaga cgtgctgcat tatcttcaat gagtttgact ttgtgttcgt gtacatctaa
    21001 agcgaccact tctccaccgc atgacggatc taaaaaagaa gcaatatgcg tcgtcttacc
    21061 tccgggtgct gcacaggcat ctaagacatg atgttcaggc tcgatttgca tagacggcgc
    21121 tacaagcatc gaactttcat cttggatcgt catgacgcca tcgtggaaca atgagctaga
    21181 agctaaatgc ccttttttcg ctactatacc gtatggactg acttggcttt tttcagctgt
    21241 gatctcttct tcttgcaaaa cagtgatggc ctgatctctg gaaagtcggc gtgtatctac
    21301 tcgggcacta gcatggctag gctgatatag cgataaaccg agcttttcag tttcttcgta
    21361 tccgatttga tccaccagtc gtttcgtcat ccatagaggc aggctgattt tgacagaaag
    21421 tctttcgata ggatcagcaa tgccttgcag atctggtgct ccttgacgct gataattcct
    21481 caagacacca tttacaaatt tccctgtccc aggatttcct tttacttttg cgatttcgac
    21541 cgcctcgttt aaaatagcat gtgcagggac tttatctaaa aattctaatt ggtatacaga
    21601 aagataaaga agcatcttga cccagtcatc cacttttttt gctttagcga tcaatggtgc
    21661 aacgtaaaat tccaatagtc gctgatggct aatcgtacca tagatcaatt ctgtaaataa
    21721 acccacatct ttttgattta gctcgctttg tttgatcatc tcattcaaca aaagattcga
    21781 gtaggcgcca cccttgttca ctcgctctaa tgtcagtaat gccatatatc gtactgattt
    21841 tttcattttt ttagggattt ttttcattat ttcacctgct gaccaatctg aacttgctga
    21901 ccgcttccgt taaggaaatc atgtacggcc tgttttcctt taccagctgg ctgtaattca
    21961 ttgatggcta agacggtctg tttaccgcaa gcaatccata gatttttttt gtctttccgg
    22021 atgatcgtac caggtgcttt gtctgtagtt tctgccaata cttcaaccga taataatttc
    22081 cagcgtatgc cctcataggt cgtgaatgca attggccaag gacgcattcc gcgtacttgg
    22141 ttgtcaattt cttctgcagt tttatgccaa tcgattgctt cttgttcccg tgtgatgttt
    22201 ggcgaaaaag ttgcttctga ctcgtcttga ggtactggtt tttgtccatc caaaatattg
    22261 ggcaatgttt ctagcaatag ctttcttcct aaaaggctaa gcttttcaaa catcgtccct
    22321 acatcatctt gtttagtgat tggtatgctt tcttgcgcat agattccgcc tgcatccatt
    22381 tttttgatca tttccatgat cgttacacca gtttctttct ccccattcat gatggcataa
    22441 tgaacaggcg cacctccccg atatttagga agtaaagaag catggacatt gattgcccct
    22501 aattttggta cttgcaataa cttttcaggc aagaattggc caaatgccgc agtgacgatc
    22561 acatcaggca ccaattcaat gatttcttcc atttctggcg aaccggagat tttctctggc
    22621 tgcagcacac ggataccgtg cttcagagcc gcttttttta ccggtgtcgg cgtaattact
    22681 cgtttccgcc caactggacg gtcgggctgt gttaccactg cctggatctc gtaacctgat
    22741 tctatcagcc cttcaagaat cggcacagaa aaagcaggtg tgcccataaa tactagctta
    22801 gtcatcaaga tgttcctcca tatattcttc aagttcttct tccgggatct gctgtatcat
    22861 tttctcaatg aacaagatac cgtctaagtg atcgatttca tgctggaagg cacgggctaa
    22921 atagccaaaa gccgtcactt ctatctcttc tccgtctcga tcataataac gcacggtcac
    22981 ttcatctgct cgcttgaccg ttccatagac atgcggaata ctgagacaac cttctacgtc
    23041 aaggctttca ccttttgctt cgatgatttc tgggttaatc agttcaaatt tctcgccttc
    23101 atctacttcg atcacagcaa ttctcttgtt ttgtcctact tgaggagcag cgatccctat
    23161 tccgtcattc gcaatcattg tctcatataa attgtctaat aaagcgattg tctcgtccgt
    23221 gattacatcg attggctgag cagttctttt taatttatca ttagggtgga tcaatatagg
    23281 atagcgcata atttggccct ttcttaaata aagtttaatg gttcgttgtc aattgccaca
    23341 aaaagacctt gtcgctgttc tttctggctt ccattcaaaa tttcatccaa aacatgagga
    23401 agattcggtt catgtttata tttgatgatt agttgataat aatatcggtt tttcactctc
    23461 aaaatcatgc tgggcgttgg ccctaatagc aagctttgag ggcttaacac ttcttttagc
    23521 tgattggcaa tctgaaagat tttctttgcc gctacttgtt cttcaggatg gctggcggtt
    23581 attttcacag taaagtaata aggaggatag cctccacgat gccgtacatg catttcctgc
    23641 tgataaaact gctcataatt ttgtttttta gccaattcta tggcgtaatg ttgaggattg
    23701 aatgtttgga tcacgacttc gcctgctttt tccgctcgcc ctgctcgtcc tgaaacttgg
    23761 gtaagcaatt ggaatgtatg ctcactagaa cgaaaatccg gtaaattcag ggaggtatca
    23821 gcattcaaca ccccaactaa tgtcacttct ggaaagtcca aacctttagc aatcatttgt
    23881 gtccctaata atatatctgc ttcttttgca ccgaattttt gtaaaatctg ttcgtgtgcc
    23941 ccttttcttc gagtggtatc tacatccata cgcaaaatcc tagcttgcgg ataaagttct
    24001 cggagttcct cttctacttt ttgagtacct gttccatagt agcggatttt attgccgcca
    24061 caattaggac agcgattggg aataggttct tcatgtccgc aataatggca gcgcattgaa
    24121 cgtgtatcca tatgcagtgt aagagagata tcgcaattag gacatggaag aacaaatcca
    24181 cagtccctac acatcacaaa agacgaatat cctcgacgat tgagcagtag gacagtttgt
    24241 tcttttttgt ctaatcgatt ttgtattttt tcctgcaaat tagcagaaaa agtagaggtc
    24301 cgatgattct gaaactcttc acgcatatct acgatctcaa tagctggaag ctgtgcagct
    24361 gctttggctc ggtggtttaa ctgcaataac tgataaacgc ctttttgagc tcgtgctctt
    24421 gattctaaag agggcgtcgc gcttcctaga actattgggc aatgatgata tttactgcgc
    24481 caaattgcta gatctctcgc atgataacgc ggtgtctcat cttgtttgta acttgattca
    24541 tgttcttcat cgataatgat cagcccgata tttttcagcg gggcaaaaat agcagatcgt
    24601 gcgcctacta ctacttgcgc ttcttcccgc tcaatcttgc gccattcatc atatttttca
    24661 ccttgggaaa gtccgctatg taagaccgca actgattctc cgaagcgtcc tttgaatcgc
    24721 tcgaccattt gcggagtcaa tgcgatttca ggcactaaca taatagccgt tttattttct
    24781 gacaaaacat cagcgattgc ctgaagatat acttctgttt tcccgctgcc agtgattccc
    24841 tccagcaaaa agacctgatc tttttgctgc tgacctgctg tgatgatttg ttcgacagca
    24901 tttttttgtt ctgcattcag ttccaatgcc gtcgtttggt caaatactcg gtctttataa
    24961 gggtcgcgat aacgttctga ttctataaac tccagccagc ccttttcttt tccttgattc
    25021 aagacagcag tactgattcc tttatttttg aaataagcaa ttggttcttc ttggctcagt
    25081 tcagataaac acaaaagaag ttctttttgt ttcttggcat tttgccgtaa ttttgcccat
    25141 tcttcttcga tttgcttttc agttaacaaa gaacggacca aacgaatcgt ttttacttta
    25201 tttcgtgtat ggacttcata tttgatatct actaattgct cttggcgcca gcgaatcaat
    25261 tgagaaaggc tgccttcttc ttctgcttcc ttccaggaaa tcacttctcg ctgatcaaac
    25321 aatacctgaa cttctggtgt gtctgccaaa ggatagatca atttatcata ttctgctttc
    25381 atcacactcg gaagcatcgt ttgtagacaa gtgattttga atgcgtaagt aatttttttc
    25441 atataatcag ctaatgaaag taattcttca ttgaccaccg gagcaaggtc tagcaaacga
    25501 ataactgcct tcaaagatgg attagctgat tcttctgatt tttggatagc cataacaaat
    25561 ccttggacat gacgattgcc attaccaaaa gggacttcta ctcgcatccc gacttgcaaa
    25621 gctgtttcca tttcagaggg aaccaaataa gtgaatggct gatcggtctg catagtaggt
    25681 acgtccacaa tgacttcagc tgttaatgtc atggttcgtt tcctcctttt tttgtctttt
    25741 cttatcaagc gacaaaaagt tccttgccta ttttactaga gatcgatgga tttgtcttat
    25801 cttctgccaa atcctaacaa aaaattaaca gcagataaga aaaaaaagaa ctgtgtcaaa
    25861 aagttcagac acagcctttt ctaccagaca ataagtggcg aatgtttgaa aaggaaacag
    25921 ctgggtttgc ttttcttttt agatgttcgc tctatttttc tttattacat tttttgctct
    25981 tcacggatac gtgattccaa ttcgtgttgt tcacgctctt tttgtgcttg acgttcctct
    26041 tcttccatct tcaaacgttc acgttttaat tcaggatgtg gatcattgac tacatttcct
    26101 gcatcgattt cttctaaagc ttgaccgacg ctttttactg aatcaaaaga atctagagta
    26161 ggctgtgcac ttgcatccaa ttcatgcgcg cgtttacttg ctaaaatcac tagagaatat
    26221 tttgaattta cacgatctaa tagtgagtca attgaaggtt ttaacatcat aagactacat
    26281 ctcctttaac attttaatat atttaccgat tacgcgatct actcgaaaat gttcactggc
    26341 aataatgttt ttgattcgtt caactgctag cggcacttca tcatttacaa cagcatagtc
    26401 gtaaagagcc atcatttcga tttcttcttt tgcaactttc attcgttttt cgattacttc
    26461 atctgcatct gttcctcggc ctacgatccg tgattttaat tccgctaaat ctggcggagt
    26521 caaaaagatg aatacaccat cgggcacttt ttctttgact tgttttgctc cttggacttc
    26581 aatctccaag aaaacatctt ttccttcatc caatgtcttg ttgacatatg acaaaggagt
    26641 tccataataa ttcccgacat attctgcgta ttccagcatc tctccagctt cgatcattga
    26701 ttcaaactct tctttgcttc ggaagaaata atcaacgcct tcgacctccc cttcacgcat
    26761 tttacgggta gtcattgaga tcgaatactg aaaatcatta tcctcactct caaaaattgc
    26821 ttttcgtact gtgccttttc ctacaccgga tggaccagac aacacgatta ataatccacg
    26881 ctctgacatg acgacgtcct ttcaactagt ctttttgtac ttatcgttta ttttgcactt
    26941 tttttctgca actttcaagg ttatctttaa ttatagctag aaattggtaa aaaagctacc
    27001 ctgtaccaag aggtcgtaaa aaagctgtcc tgcatcaagt aataagaaca gccaaacata
    27061 aacagcttct catgtttttc gtttggttgg ggttattatc gcagatgcaa gcttttgaac
    27121 gccgtgtata agaggtcgtg aagaagccga ttagtttcga gtatgaagaa aaattttgtg
    27181 aaaatagttt ctcacatttt tgcataaatt tggcttaata ccgagaaaat ggtttttgaa
    27241 catcacttat tcaaaataaa aaaagattgt gacacgaatt ttgtcacaac ctttaaatgt
    27301 attcctagag caaagaagct aagcgaagaa atccaattct gtttatttat tgtatttctt
    27361 tgttcagcat ccttgctaaa agcccatatt ggacaaagta ttcactacac ccatgatctc
    27421 atcttttcga gaacgtgctt tttctgtcgg atcttctacc tcttctgtgt caattcgaac
    27481 aataaatttc cgattcatcg tcacgtaagc accgtcgttg atcaaaataa acagttcgtt
    27541 ctcattatag tctgcacagg catctttcca tacgtcaaag ccgttttttt cagaagggat
    27601 atcccgttta accacagttt ttccacttaa ttcgtggaac gttacgtcat aatatttcat
    27661 tatctctacc tctttcgtca ttcaatattt tgtacttgtt ctctgatttt ttctaagatc
    27721 gtcttagact ggacgacttg atttttaatt tcgattgcat ttgatttgga accgatcgtg
    27781 ttgatttccc gattcatttc ttggatcagg aaatctaatt cccgacctac aggacattgt
    27841 gcttcgataa gttcatctag tttttgtaca tggatcgcca gacgatccag ttcctcgtga
    27901 atatctccgc gttccaacaa gatggctagc tccgtcaaca accgttgctc atcgactttc
    27961 gctccaagaa attcctccag ctttttttga taccttattt gaaattcctt ttcatagatt
    28021 tctacaaaac tatcgagttt ttttagtact tctttgaatt cttcagtatt ctttttcaac
    28081 actaaagcta aagcttgacc ttctttttct cgactcgcct caatagaatc aagtgcgctt
    28141 ttcaatgtat ctagagctaa ctgtgccaat tcttccatgc tctcttccgg tttttcttct
    28201 accgttacga agttttcgtc cagcacgaag cgttccgcca tccgactaat tgacacttga
    28261 ttttgactac cgtatcgttg ttgaattccg tctgtcaatt gagtgataag tgaatcaatc
    28321 aattcccagt tgatgacgat ctgtttttcg tttttgccaa tataagctag attcacaaat
    28381 acttcgattc gaccgcggct taactgctgt ttaataagct gacgaaaatc gttttcaaca
    28441 aagttcagga ttttaggact tcgtatttga acatctagaa aacgctggtt gacacttttt
    28501 atttcaatat ctaactgata cgcttttgtt tcgcggatgg ctttgccaaa accagtcata
    28561 cttttcattc gacagggtcc ttcctcatta catattttct attgcttttt gcagttgttc
    28621 aaactcttcg ttcgtcaatg tcaggccttt ccccattttt tcatggtctg gcgcccaatc
    28681 tcgaagatcg aattttggcg ggcgaccatt ccagctgact aggttcagtt ctttacgcca
    28741 ccccttgttg ttttctgaca atatagcgat ttcttctacg atttcatatg aaaattcttg
    28801 tgccatggtt gttcctcctt gagataatat aaatatagga tcagtaatgt gtcaactggt
    28861 tgcttaagca attcatgctg caaacgatcc ttgcgttttc cttgtagcag ataatcaaaa
    28921 taaacagttt tcgtgcgctc attttgtatt ttttgctggt attttcgata ccttcgccat
    28981 tcttcagtac ttaattgacc aatttgtgcc aataaatcat ccggcatact ttttatatct
    29041 tgttggcaga tcatttgaat catcatgtct acttgataga tttcattggg cagtaaaaaa
    29101 tgtttttttg ctaaaagttc tagcatcgcc aatccgcatg tctctggtac gaccctgttc
    29161 ttatataaat tacgcagttt ttgccaaaat tttatgcttt tgaaaaaaga taattctaac
    29221 tgaatttctt ttgttaagta agacaaaaat cctcgccagt atgttcttgc aggtttaaca
    29281 gaatgaaatg tataccagtt cccatagtaa tagcctaatc gttttttcat ttccagatag
    29341 cctaattgta aatttaattg gttcctttgg ctatcaaaaa gcagaaagct gcctagtgtc
    29401 cacaatgttt gacatttcca atggataaaa atgtctggca tacgaatttt tttcgctggg
    29461 cctggccctt gcacatctac gacaaatgcc tctgttgctc ctttgttaat cgcaatatca
    29521 ataggtattt tgttgcgata cccaccgtct gcatatttat tttttccgat ctttcgataa
    29581 gccatagcgg gataaaatga agcagaggcg agtatccaat ctggtatttc ttctgttttt
    29641 gtctgttgga tatcagtcac tacttccgct agttctggca gtttagtaga caccgtatat
    29701 aaacgtattt tgggattatg tttgagtaag gacaaatcca aattgtcttt gaccatcttt
    29761 tccaatggag cagaagaaac cccgccttca attagtgctg ttttcgtcat ttggcgtctt
    29821 tctcgataga agcgttcacg aaggtcttca actgcagcca tttctggtaa cgcaagcact
    29881 tgtcttgtcg acagcttttt ccataaattc acagcttttt tcatatctcc ttgtaaaatc
    29941 aatgcgccgt tcaacgcacc taccgaggta cctgtgataa tctcaaagtt gatatcatgt
    30001 tccttcagcg cctgccacac accaatctga taagcaccgt gcgctcctcc tccacctaaa
    30061 acaagtgctg tatgatagac cagcttttta tttaattgtg tggttccttg atagccgttt
    30121 ttatgaaaaa tacgcaacca ttcttctgat agaggagatt gaaataaaaa acaacagttt
    30181 tctttaataa aaaaacgagc gcaagcttcc atataccgaa aaaatttttt ccattcaaaa
    30241 gtttcacctt caatcaacag attattaaaa ataacatctc ctctactttt agcaatagta
    30301 aaatagacgg tatatttttt cgatactgct ttaaaagcac attgcttttt tgcagaacgc
    30361 caaaaagcgt ctctactttc ttgatgactt tgaaaaaatg gaaggggttg ttttgcttgc
    30421 tggataggcg aatacagcga cattttctgt gaaaagtaaa ctttttttat ctccatcgcg
    30481 ctctcctttt tcttcaagta taccagtacg tcataaaaag gtcatgagtc tcgtattcga
    30541 ctttgtagat tccataagaa aacaattcat aaaacctcct ttaaataaaa aaatacaaaa
    30601 atcacaaaaa ttaattgaaa atgagagtgt tttcaatgaa aacgtgattt tgtaatggaa
    30661 ttttaaaata aaagaaacaa gcttttttaa tgcaaatgtt gtaaaataaa agagattaag
    30721 aggaaataag aggaggaaca tgatgactga gtcagtttat gtacatattg ataccacaag
    30781 taatgctgtt ttaacaaaag ggctgactgc tactgacttt gcaaacagta tcgtgcattt
    30841 tcctcaaaac ttgctcttgc ttgatccatc tgcttctgct ggagaatatg aatcccatac
    30901 agggctgaaa gtaatccgtg gtacagaaaa tattcagcgt ttcttttcct cttctcgtaa
    30961 caggcgtgga ttcgatgaat tgaagtggat cgactttact gatttaacga tgctgaaaga
    31021 attgacacct ttagaaattt cagaattatt atattttggt catatgaaaa cacatcttca
    31081 ttcgccgttt ttttataaac tacagaataa ttttgtatac ttcgatctta atgatcagct
    31141 gaatcggatt tattatcgct atttagatga attttatagg atttttgcta gtaaattaag
    31201 ttcgatcttg tcagataaga tcaatgtaaa aaaatctttt ttcaaaaaag aaacacctgt
    31261 tgaaaaaatc agtgtggaat tattgaaaga aatgcgtaat ttgatgcagg aagggatcat
    31321 tttttctttt gatcaagttg gtttgatcga tggtaaatat cgtatcccaa tctatgtgat
    31381 tgaagattat atttggaaat taaaaggcac ccggtatcga gaagaggaaa tcatcgctac
    31441 tttaatctac tctgtcaccg atcgacactg gcaattggta gaagatgccg atctttctta
    31501 tctgccattg aagtagatct atttcttatc aaaaaataaa aaggaaacaa gaaactagga
    31561 catgattctg tctcaagttt cttgcttctt ttttatttac agctcctgtc gtttatctgg
    31621 cacaaaaatc gaaggagtcg tttgttggat ccctaataaa acgatgatcg ttacaatagc
    31681 tgtactcaac ccgctggcac cattcattac gaaagaaaag agccaaggat tcatccccca
    31741 tagtgcatac gctccccaga aaaccactcc tgcaataaaa tgccaaaaat atctggaaag
    31801 tgcgccaatc aatgcaccaa acacgatgct ttttctaatt tgtttcgtat tatcgtctac
    31861 atagcctttt tggacatttg aagccatgag ccctgctgcg cctgcaaaag tataggcaat
    31921 cgggtactca atcaacactt gaacgacact taaataataa acttgtccca acggaaaatg
    31981 taggatgccc caaatgaaac ctgccagcat ccctgccttt gtccctcttc ttaacgcaaa
    32041 aagagtcaat ggaatctgcc ctaatgaaat agaaaaactg ctgccgatct ggatcggaat
    32101 caacgacaac accatcgcta acgctgcaac aatcgttcct tctacccaaa ctctattttt
    32161 tcccataaaa aaacttcctc ctttgaataa aaaagtcaca aaggaggaag cagctttcga
    32221 aaatagtcaa acagctgttt attcacaatt cctacgctcg cataaccgat acaggtacga
    32281 agggtttaga aatagattca atctcagccc aattggactc ccctttgtga gtttatttat
    32341 tatttatttt ttgtttcaaa caatggacta actggtttgt tttcatggat acgtttgatt
    32401 gcatctccca ttagtccgcc aacacttact tcatcgattt tgtcgatttt acgatcttct
    32461 ggaagataga tcgagtctgt aacaactaga cgctcgatag ctgaatcttc tattctttgt
    32521 aaagcagggc cggataatac tggatgtgta catgaagcat acacactagt tgcaccagct
    32581 tctttcaaag cattggcagc caatgtgatc gtacctgctg tatcgatcat atcatcgatc
    32641 agtacacaag ttttgccttt gacttgtcca atgatattca ttacttctgc aacatttgct
    32701 tttggacggc gtttgtctat gatcgcaatc ggagatttca aaaattccgc taatttacgt
    32761 gcccgtgtga caccaccatg gtctggtgaa acaacgacaa catcatcgcc tttgattcct
    32821 ttttctaaga aataattggc aataagtggc gcacccatca aatgatcgac tggaatatca
    32881 aagaaacctt ggatttggac tgcatgaagg tccaaagtca gcattcttgt tgcaccagct
    32941 ttttgaatca tgtttgctac taatttcgct gtgattggtt cccgtgcacg tgctttacgg
    33001 tcttgacgcg catagccata ataaggcatg actacgttga ttgtttttgc acttgctcgt
    33061 ttcagtgcgt caatcatgat cagcaactcc atcaagttat cattgactgg gctgctcgtt
    33121 gattgaataa catagacatg cgatccacga atactttctt caatgtttac ttgaatttct
    33181 ccatcactaa attgagtaac agatgatttt cctaactcga cgccgactgc tgctgcaatt
    33241 ttctctgcta atgggcgatt ggaatttaac gcaaaaattt ttaatcttgg gtcaaagtaa
    33301 tgttttgaca tggggacctc cactttcttt ttcacaactc tagaattcta cctaataaat
    33361 agtagtcata ttttttaaat aaatcaagtg attttgcaaa agtcttagtt tagataaggg
    33421 agtttttttg catatccttc tttattgact tgtcttgctc tcgcaattgc cagcgcatat
    33481 tctggaatat tgtctgtaat tgttgacccg gcagcaatcg aactgttttt agctacctcg
    33541 acaggtccga tgatattcgt gctagaacca ataaagctgt ggtcgccgac agtcgtatga
    33601 tgtttgttct ttccatcata gttgacgaag acaacaccac atccgacatt gatttcttct
    33661 cccagcgtag catcaccaac ataagtcaag tgaccgactt tagttccttt gccgatttga
    33721 gcatttttca cttccacaaa gtttccgata tggacgtttt cgccaatttc agcttttggt
    33781 cgtaaatgtg cataaggtcc cacatctgca tgtttcttga catggctgct ttcaatgact
    33841 gagttttcaa tgacgacgtg atcctcaatc ctactgtcta cgatttttga atgtgacccg
    33901 atgacacaat cagaaccaat gactgtattt ccttggattt gtacacctgc ttcgatcaat
    33961 gtgtctggac cgatttctac gcctgcatcg atgtatgtcg ttgctgaatc gacaaagctt
    34021 acgccattga ccatatgcat ctggttaatt cgctttctca tgatctcatt tgctttagct
    34081 aacgcgatcc gatcattgac tcccattgat tcttcaaaat catccgtttg ataagcagca
    34141 actgtatggc cttcttcttt taagatttcg atgatatctg ttagatagta ttcaccttga
    34201 gcattgtttg ttccaacttt gcttaacgca tcgaacaaag cttcgttgtc aaaacagtaa
    34261 gttcctgtat tgatttcttg tacaagagct tcttctggtg ttgcatcttt ttgttccacg
    34321 attttttcta cgatcccaat atggtcacgg atgattcgtc catatcctgt tgggttttct
    34381 gcttgtgccg tcaagatcgt tgcacttgca ttttttcctt ggtgatattc aaaaagattg
    34441 ttcagcgttt cagtagtcaa taatggtgta tcgccactga taactaacgt tgttcctttt
    34501 tttccttcca agaaagaagc agcttgcaaa acagcatgtc ctgttcccaa ttgttctgct
    34561 tgtaacgcat atttactgcg ttctcctagt tgtgctttaa cttgttctgc tccgtgtcca
    34621 acgatcgtaa tcacttcatc tggtttcgtt tcactgaccc gattaatgat atgttctacc
    34681 ataggtttac cagaaacggg atgtaaaact ttatataatt ttgacttcat gcgggtacct
    34741 ttccctgccg ccaaaataat tgcataacgt gcgtccaact gcgtcactcc taaatatagt
    34801 ttgctgattt cagtttatcc cagtataccc attttttcaa caaaacaagt gattttcttt
    34861 tatcccagcg ttttctggat aaaaaaatag atagaccata acgggcctat ctatttggta
    34921 accggtgctc tcctgccaac tcctcggtca caagtcacac tactcataaa acatgaaaag
    34981 ctgttttttg aaatcgtttc ttgtgactta gagttaaacg gtaggcttac aacctcttca
    35041 ttaaacgttt ttcaatcagc tgaccttcgg tattagctca ataaacggca aaatatgagg
    35101 agcaattttt ccgtttattg agctaatact caggacagga cgctgatttc acaacctcta
    35161 gataaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatga aaaaatgtgg
    35221 aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcggctttt tcacaacctc
    35281 ttattctgaa aaataatttc ctggcacaac cgtaatattt ttcgtttgag tatctacatc
    35341 tttgacataa agcagtgacg tataatcatc gatggcgcga tgaccgttga attttgattc
    35401 ggcaaataca gtgatgccaa caagttctga ttcgaattct tcaatcaagc tcttcattcc
    35461 gttgactgtt ccgccgcctt tcatgaaatc atccactaca aggacacgtg aaccacgttt
    35521 caaacttctc ttagacagtt ccatcttttc aattctttca gaagatccag acacatagtt
    35581 gacacttact gttgatcctt ctgttatttt tgagtcccgg cgtacaataa cgaatggcgc
    35641 attcagataa taggacacag cttgggcaat aggtacgcct tttgtggcta cagtcattac
    35701 agcatcgatt ttttcaccaa ggtatttaga agcaatgatt cttccaactt gacgcagcag
    35761 ttcaggttct cctaacaaat cagagagata aacatatcct cctggaagca gacggtcttg
    35821 ttctgataga cgttctgcta gagattcaat atattttttc gcctcttcat atgaaatttc
    35881 agggatgaac aatacaccac ctgcagcacc cgggattgtt tccaaggttc cataccctcg
    35941 ttctttaaat gttttcttga tgattcccaa atcttcactg atagaagatt tagcagattc
    36001 ataacgttcg gcaaagcttg ttaaggaaat caattcatgc gggtgattca ataaatactg
    36061 tgtcatgtca actagacgtt cactgcgacg tactttcacc tattccaact ccttttaaga
    36121 aattttatcg ttttacatta tataagtttt tcttcaaaaa ttcgagcatt ttatgaaaaa
    36181 ataagaaaat agttcggtat ttattaaaat aaaaagaagc ctataaaaaa tttttcgttt
    36241 ttataaggct tcttttcttt ataatttagt tattttttct tctgaaggca accaatattc
    36301 gtttgatgat atttacaatc aggaacaaag caataaagac taatgtgatt gttgctcctg
    36361 gaggtgtatc tagataaaac gagctagtta gtcctgacag catccctaga aatcctacac
    36421 aaacactgat agcaatcacg acattgaagc ttttgcctaa tcgcataccg attgctgcgg
    36481 gaagaaccat gattgcggat atcaaaagtg caccggcaat cgggatcatc acagcaatag
    36541 ccacaccagt aatcacattg aacatcatcg acatcaaatg aacgggtagc ccatccacgt
    36601 gggcggtatc ttcatcaaaa gtcagaacat acatcggccg tttgaataaa aagaacaata
    36661 taccgatggc aacgaataag attcctaaaa agatgacttg ctcccatgta atggtcacga
    36721 tcgaaccaaa cagataggat tggatacttg ttgctgaatt ccctccgctc aaattcatca
    36781 atactaacgc tagcgccaaa ccaccagcca ttaaaatggc aatggagatt tctgaatagg
    36841 ttcggtagat cgttcgcaga tactccaata tcacagctgc taatacaacg actagcatgg
    36901 tcgtaagtgt aggattaagg ttcaaaaaga atcctaacgc tacccctgct aaagagacat
    36961 gagaaagcgt atcagccatc agcgattgtc ttctgatcac taaaaacaca cctagcatcg
    37021 gggcgattcc ggcaatgaag atagcggcaa gaaacgctct tctcatgaat tcatatgaaa
    37081 gcaacgccat tgagaatcct cctttctcac cagtcggatc tgtctgtccg catactgttt
    37141 gatatcttca tgatcatggg tgatcattag aactgatttc ccatggacat gcgcactatg
    37201 tcggagaaga cgataaaatt catttctgga ttcttcatcc atacctgttg ttggttcatc
    37261 aagaacaaat aagtcagggt cagtagcaaa aatacgcgct aaactgattc gctgcttttg
    37321 tccgccagac aattcaccta ttcgccgatt tcgcatatcc cacatcccta cagcttctaa
    37381 agcacgctgt acatgttgat gatctttttc agagagacgc ttgaaccagc ggcctcttgg
    37441 atatcttcct gaacggacta attcgatcac cgtactagga aaacctgcat tgaaagatgc
    37501 aacttgttga ggaatataac cgatacttaa tttttttcct tcttgatttt tctcagcaat
    37561 ctttatcatt ccttttgaag gtttcagtaa tcctaacgat gctttcacca aggttgactt
    37621 ggctgctcca ttttctccag ttaaaatgac aaattcaccc gcatctacat gataagagac
    37681 atcttctagt acaggttcat catcatagta aaaggtcaaa tcagatattt cgatataacg
    37741 caccttacaa cctccattct aaaacttaat tgatactttc ttttaaagct ttcagatttt
    37801 tttccatgac agaaatataa ttttcgcctg ctttgatttg ttcattagtc aagctttcta
    37861 aaggattcaa cacttctagc ttcactcctg tttcattcga caatgtctca gctaccttcg
    37921 aagaggcatt ttcttcaaaa taaatcactt tgatgttgtt tttcttcaca aattctttta
    37981 gctcagctaa gcgacttggt gtgggttcct gatctggtga aacgcccgaa atcgctactt
    38041 gttttaagtc gtattgattc gctaaataag caaaagcagc atgctgagtg acaaatgtgc
    38101 ggtttgttgc atcttttagc tcatcggagt atttttgatc cagtgcttca agctttttga
    38161 tataagcatc ggcattcttc gtaaaagttt cctttttatc tggatatttt tcgcctaatt
    38221 cttcagcaat cgttctgact tcttttatag ctaataccgg atcgagccaa acatgtggat
    38281 cgtattcatg tgaatgacca tgcccttctt ctgattcttc atgttcgtct gcttcttgcg
    38341 attctaaacg gtcgattcct ttagctgctt cgatcatcaa ggtctgtttg ctatccactg
    38401 aatcctttaa cgaatccaca aaaaattcca tgtctgaact attatacacg aaaacatctg
    38461 catcggaaat ttctgctctt tcttttgcac ttggttcgaa atcatgcggc tccgttccag
    38521 cagggatcaa aagtttcacg tttccttcat cccctacgat ctcttttgta aaatcataca
    38581 tcggataaaa agtcgtaacg atcgtcaaat cctctttttt gtctgcttcc ttgttcgtat
    38641 ttccacatgc tgcaaacagt aaagcaccta tcattaccgt agctgtcaaa agatattttt
    38701 tcatttatat gctcctttca atgaacaaat cggaatttta cgatttatta acgtgaacta
    38761 atgtatcaaa ataaaaaaac acagtcaata gaaatccatc aatttctgat ttaagcatgt
    38821 atattcttga ttaaatcgta aaatggatga gttgctgctc atatctagcc atttcactac
    38881 aataaaaaac tgaagaatac cattttagga ttcctcagtt tttcatcacc tattttactt
    38941 cttatatttt attgcagggt tcgtactaaa taaacttcct cacaaaaacc ttttaaccca
    39001 ttatatactc tctgcgctct tgaataatgt tgacacaagg caaaaacggt tggtccgctt
    39061 ccactcatca atgcagcatc tgctccgtac ttgatcatac gctctttgat ttgatggatg
    39121 actggatgcc gctttcccgt tatatcttct aaactgtttc ccattttttt gatcatctgc
    39181 tgataatctt gctctcggat ggcttcagca agtccgtgga tgtcttgatg ttggatggct
    39241 tgcgtatcta cttctctaaa cactgtacgt gtagagacac tcattcttgg tttgaccaac
    39301 acaacccagc actgtggcat agaaggtaat acttggattt cttccccttt gccaccgaca
    39361 aatgccgtac ttccgtagac acagtaagga acatctgtac cgacttccaa accgatttct
    39421 gccagttctt ctaacgaaca gcccagattc cataattggt tgatccctct tagagcagct
    39481 gcacagtctg tacttcctcc gcctaatcca gctgctactg gaatattctt cttaacaaag
    39541 atcttgacac cggttttgat attgtattta ttttttagca ataaagcagc ttgataaaca
    39601 ttgttccgtt catctaccgg taaaaatgct ttatttgttt ctacaatgat tttgtcttct
    39661 gttatttctt caaaaaatag atgatctgcc aagtctacgc tcgacatcac catttccaac
    39721 tcatgatacc catcttgccg tctatataat acatccagtc ctaaatttat cttggctggc
    39781 gctttttcaa tcaattccat cctctcaccc attcgaaaag tctaagtcgc aagtattcta
    39841 tcatagggaa aaataaaagt atagcttatg tctgcctagt ggttgatttt gtcatcgttt
    39901 caacagcttt cacagaattt tctcttcata atccaaacaa gttctagtga ttcttataat
    39961 tctgtgagca cttcttctgc caaactcgga acatttcctt tttcttttcc tatcagactg
    40021 agtagctgca tggtagcaaa tccgaaatta cttcctttcc tataagcaaa ataacgtcct
    40081 tcccaatgat cgacatattt taatttcttt tcataagcag actggaagga atagaaaaaa
    40141 ttaccataat tatatacgat atgcatgact ttttcttgaa agaaagaaaa atctgtttct
    40201 ccaacattag ctaatggagc taagcctaga ttggcttccg agtagcccat tttctcgtat
    40261 acaataaaca gattggctaa cagatagtca gctaattcct cagattcttt aggtaagacc
    40321 cgcaacaaat catatcccgc ctgcttcttc gtcatcggct gagcggtgat gaagccaacg
    40381 actttttgat ctttacggac aataccgaca ccgctagata atacatatga tttatcaaat
    40441 cgtcccacag agaagtattt ttctcgtgca ccttgaagcc atttttgtga aacttcattt
    40501 acagcttgat gtaattcttc cggcagttga tcgtaccaag taaaatggta tccttctttc
    40561 atcaaacgtt gatattcaag ttgctgactc agccgagaca agtcttctgt tgctagatca
    40621 acaatccctg cttcacctac tttcgtgaag tgaaaaccca attcatgaag catcactacg
    40681 tagctttctg atatcttata aaacgcaagc tgatagccta aacaatctgc ttgtttcata
    40741 aactgattcg ttgctgcccg catcatttta ggattgccaa ctggatctcc taaaacaaag
    40801 catttgtttc ctttgatctg ataaccaaaa acgacttgat cttggtcgtc ttcttggtaa
    40861 taaaaataac tgtaacctga caagcgaagc tgatggcttg tagctgtgcc tccgtatgtt
    40921 tccaataaac ggtcaaaacg gtctttttcg tactcactgc caagcttcgt agtatcttca
    40981 gctaaatatt gataaagcgc cactaaagct agcaatgaga tgcccaaacc aataagccct
    41041 gagaaccaca catcatctga aggaaacaaa ataaaatgat ttcctgttat tcgaccgact
    41101 aaaaagcgtc cgctgtgata gcctgcaatt gcgtaaacga taaacaaaaa gccgaacaag
    41161 ctagcatcaa agattatagc tccccaagaa taaacgaatt ttgttcgata aaattctttt
    41221 ctggataacc acacagctaa cagaatcaag acatatacca atatcaactg ccaagataac
    41281 gtacgtgtga ttgtatttaa aataccaaaa gatagtaaag caatcgtagg cagaaatgcc
    41341 tttttgactt ttactgaaag tgctctagca aggccgagta ataaaaagcc aaccaataga
    41401 ttcactgtct gatctaaaaa attgaatgag aacggcaata atacttgaaa tagccgactg
    41461 attgcagaca gattagtgat tgttgataga agtaccatca tgatccccgc aaaatagaca
    41521 gcacttacga gcacgatatg cgcaaccttt tggaaaaaca gtcgcggaat gttatctaaa
    41581 aatcgattga ttttgactcc tgtatgtgtg atgaacaata gaagtcccgt cataaaaggt
    41641 agaacataat aaaacaaccg ataatataat agccaaacta ctgctacatc ttgccgaaca
    41701 gacaattgac tcattcctaa gatcatcaaa acgtcaaaag tccccattcc acctgggacc
    41761 atcgtcagca ttcctatcaa tgtagcaatc atgaacatag gatagatcga aagcatcgaa
    41821 atatctattc tcatcaactt gccgatgatc aaaaataccg caagggctcc cgtccattgt
    41881 ccaaaggatg ccagtagcag accaagcaag ccttttggat aaaactcgaa gaacaatgtc
    41941 tttctcttta gataagcaaa aaagaaaatg actggtgcta aaaaactacc gccgaaaagc
    42001 cagatccaat agcttcgaaa aatactttct gactgaataa aaaagacatc aataaatgta
    42061 agaaacgacc atacagacaa accagtcagc ataaatagtg cgacttttga tacagtggct
    42121 aaaaccattt ttctatttgt ctgctttcca taaaaactag cccgcagact tgctcctacc
    42181 acaccaccaa aaccggcgag attgttgatc gtgtttgtta cccacgcagc aagaagaaac
    42241 tcttttcttt ccatttttgg ttttccttgt ttttctaaga ctctgatcgt cacccaatca
    42301 tacaacaaca tcggagtgac acccagcagt ccagccagaa tcatgagcag gatcgtcatg
    42361 cggctttgtt gtcccatcct ttgccaaata tcttgccaac tcatgccatt agcaatattt
    42421 gcgacttgat ttgccacaaa gactaacacg gagccgaaaa aaatcagttt taaaatcaga
    42481 ctatgttcct ttatccattg cagcatcaag tatcgtttat ttttcacatt ttcccattcc
    42541 taacttgtat acttttgtgt cataccccaa tgttatctta tttattttaa tttttccact
    42601 ttagacctgt tctttgcttg cgatgataga aaaagaaaga tatcctaatg ctaaaggaga
    42661 ggataacatg aaagtaggat ttatcggcac agggaatatg gcacaagcta ttattgatgg
    42721 tttattaaaa aaaggaacag tctcgaaaga agaggtgttc gtttccagtg gacattttga
    42781 aaatgcacaa aaatttgctg ctgaaactgg cgtccatgca tgcaaaagta atcaggaagt
    42841 tgcatcacaa tcagatatcg ttcttttagc cgtgaaacca aaaatcattg aaaaagtatt
    42901 ggaagaacta agagaatata gccagaaagt cttattcatt tctattgctt caggaaaatc
    42961 attaatagat ttagaaacag cgcttgaaga tcctcaggcc tccatcgtac gtgtgatgcc
    43021 gaatgtcaat gtcagcgtag gagcaggcgt ttctgcgatt tgcaaaaatg aagctgccac
    43081 ggaggaacag cttcagcaag ctacgatgat ttttgaagca atcggcagcg tctatccttt
    43141 agcagaatca gatttcagta cattcattgg ccttgctggc agctcacccg cattcgttta
    43201 catgctgatc gatgcgatgg gacggactgg tgtactgaat ggtttgtcca aacagacagc
    43261 cactgagatt gctgcaaaag caattatggg ttcttgtcaa aaattccttt caagtgataa
    43321 aaatccatgg gaacttgtag accaagtttc ttctcctggc ggtacaactg ttgcaggtgt
    43381 agttgcctta gaagaagcag gattcatccc agcaatcatc aaaggaatca atacgacgat
    43441 cgagaaagat cttgagatgt tgaataaaaa ataatgatag caatgttttt tcgtgcctta
    43501 caaaaaaaga agagctgtgg cttttgccgc ggctcttctt ttgtattttt tattatttag
    43561 cataatcagt tgcacgagtt tcccgaataa cgattacttt gatgtgtcca ggataatcca
    43621 gttcttcttc gattcgttta cgaatatcac gtactaaacg aacagcgtct aaatccgaga
    43681 tttcttctgg cttgaccatt acccgaactt cgcgtccagc ttgaactgcg taacttgatt
    43741 ctacaccggc gaagctgttc gaaatatttt ccagacttct taaacgacgg atgtagtttt
    43801 ctaatgattc acttcgcgca ccaggtcttg ctgctgagag cgcatctgct gcagctacaa
    43861 ggacagagat aacagaagta gcttccacat ctccatgatg agaagcgatc gcgttgatta
    43921 ctactgggtt ttctttgtac ttggcagcta attccgcacc gatctcaacg tgagatcctt
    43981 caatctcatg gtcaagtgct ttcccaatat catggagcaa cccggcacgt tttgctaatt
    44041 ggatatcttc gccaagctct gctgctaaga taccagctaa ttttgcaacc tcgaccgaat
    44101 gcttcaagac gttttgtcca taactagttc tgaatcgcag acgtccgatg atcttgatta
    44161 gatctggatg aagtgtatga gcaccgactt caaacgctgc ctcttccccg tattcgcgaa
    44221 tacgttcatc catttcttta cgagatttct cgaccatttc ttcgatacgc gctggatgga
    44281 ttcgtccatc ttgtatcagt ttttccaagg tcatccgtgc gatttctctt cggattggat
    44341 cgaatccgga aagaacaact gcttccggtg tatcatcgat gattaaatcg ataccagtca
    44401 gcgtctcaag cgttcgaata ttacgacctt ctcgtccaat gatacgacct ttcatttcat
    44461 cattcggaag ggtaacgact gaaacagtcg tttctgaaac ttgatctgct gcgcagcgtt
    44521 gaatcgctaa tgataataag tttttcgctt tgcgatctgc ttcttctttg gcacgctgtt
    44581 cagattcttt taccattaga gttaactcat gattcagctc ttcttcagtc gatttcatga
    44641 tgacatctcg tgcgtcctct tttgataaac cagcaatttt ttctagttct ttttgttgat
    44701 cttcaatcat tttttcaacg tctttttctc gctcttcaat taattgctgt tttgaactaa
    44761 gtgcttcttc tttactttca agtgagttct cgcgtttttc taacgagtca tctttacgat
    44821 tcagcgtttg ttcgcgttga atcaaacgat tctcttgaga ttttaattct aacttacttt
    44881 ctctcagctc actttcaact tcagaacggt atttctgatt ttcttcctta gcttctaaca
    44941 atgcttcttt ttttagagtt tctgcttctt tttttgcact atcgatgatt ccagcagctg
    45001 atgattgtgc accagctatt tctttctcgt gacgtgactt tgcgaccata aaccctaaac
    45061 caagaccgac aattaaaccg atgatagcga ggagaatgtt gaataccata aaatccacct
    45121 ccatactatc tttttcattt ttatcatcta tacagcttct atgataaatt attctaaact
    45181 cgtcaatatg catcttcgca tatatgtgta caaaaaacat gcacaacttt attctaatgt
    45241 ttgtaccccg tagtgtcaac tttaaataaa caaagaaacg gctttctact tattcattaa
    45301 acgatggttt ttaaaataaa tgacactagt ttcatatagg taaaccttta aattgagaaa
    45361 cttttctatt aaattattct ttgtagatga acagatattt ttattttatg tacccactcg
    45421 tataattaaa taactcacga tttctttatt caataaaaaa acggcaggtc aaggagagta
    45481 cctactcttc agaccagccg tttttgttta tcataatttt tattcttcta ctaaaggcaa
    45541 ttcttcttgt cctttttcat cttctgggac agcgacttct tcaccaatac cataagctgc
    45601 tctaactaga gcagaaactt ctgccatcat ttcaggatga ttagccatat agattttagc
    45661 gttttcccgt ccttgaccaa tccgatcttc tttataagaa taccaagcac cgcttttatc
    45721 aacaatgtct ttttctactg ccatgtctag aagttctcct tcttgtgaga tccctaaacc
    45781 atacatgaca tctacttcag cgattttgaa aggaggtgct actttgtttt ttacgacttt
    45841 gatctttgta cggttaccaa caatatctgt accttgtttc aattgttctg cacgacgtac
    45901 ttccaaacgg atcgttgcgt agaatttcaa tgcgcgtcct ccaggagtga tttctggatt
    45961 cccaaacatc acgcctactt tttcacggat ttggttgatg aaaatagcga ttgtttttgt
    46021 tttgttgatc gaaccagaga gtttacgcaa tgcttgagac atcaaacgtg cttgtaaccc
    46081 gacatgtgag tcacccattt cgccgtcgat ttctgctcgt ggaactaaag cagcaactga
    46141 atcgactact acgatatcta cagccccact tgatactaaa gcatcagcga tctctagacc
    46201 ttgttctcct gtgtcaggct gtgaaagaag tagttcatcg atattcacac ctaatttttg
    46261 tgcatattgc ggatctaacg catgctcagc atcaatgaaa gcggccgttc cgccattttt
    46321 ttgtacttct gcaatagcgt gtagtgcaac tgttgtttta cctgaacttt caggtccata
    46381 tacttcgatg atacgtccac gggggtaacc gccgacacct aaagcgacat ctaacgcaag
    46441 agagccactt ggaattgtag agatttgttg gtcgattttt tcgcccaatt tcataataga
    46501 gcctttacca tagctctttt caattttttt cagtgcagca tctaaggctg ctttacgatc
    46561 atctgccaat acgataatcc tccttatatt tacaaatcag agcaacattt cattatcctt
    46621 atcatatctt ttttagtgaa aaaaagcaag aaaaaaccga acaaatattc gcatttatca
    46681 ttttcttaaa agctgttttc tcaaaagatc aaatccttgc ataaccgcat tatatctgat
    46741 agcagcacga tctctggcaa agtgatattt ttccgaaacc acgccgtctt tagatgaaat
    46801 agcgatccat actgttcctg cagattgacc ttctaacgtg tccggtccag ccactccggt
    46861 gaaagctaca ctgtaatctg ttcttgctat ctcacgtgct tgaatggcca tctgctccac
    46921 acattctttg ctgactgttc catattctgc taatagctcc ggatcaatct ccagaaaacc
    46981 agcttttgtt tggagcgagt aggtaacgaa tccgcctgga aatacttcag atacaccagc
    47041 aatgtcgcct aatgcggctt ggaaggcgcc ggctgtcaaa ctttccgctg ctgttacagt
    47101 ctgcttgttt tctttcagta gttccactac gacttttgct agcgaattat catcgccgta
    47161 tccataaaaa tattccccta ctcgttcttg gattttttct tctaatgcta aaagagcttg
    47221 attccctgcc tgaacatcat ttgttttcac tgtcagtcgt aatgtcactt catttggttt
    47281 ggcatatggt gcgatcgttg gattgatctg tgtctcaatc agatctttta gctcagtgac
    47341 cagtcgcgat tcaccaatcc cataaaagcg caaaacttta gaaattagtt tttcttcaga
    47401 aggaaaacgt tcctctagta aaggacgtac ttgttccaca aacattggtt tcaactcatt
    47461 aggcggaccg ggaagaagga tatacgcatg tgtatctgtt tgataaaaag tccctaaagc
    47521 aagccctgta cgatttggta gaggaacccc gccttcgata atctgggact gttgcagatt
    47581 gtttttggtc atttttcgat gggttgtttc aaaatatgcc aataattttt tgtatccttc
    47641 cgtattttgt atgagagact taccgaggtg agccgcagtt gcttcttttg tcaggtcatc
    47701 ttctgtcggt cctaatccgc cacataagag tatgagttca cttcttgttt cagctagagc
    47761 aatcaactct tctaaacgct gctgattatc tcctacaact gtttgataat aaacttcgat
    47821 acctagatca gctagctgtt ctgacaagaa agtggcattc gtattcacga cttgtcctag
    47881 taaaagttct gttccgacag ctattatttc tgctttcatt acttcccgct cccttatata
    47941 gtatacgctg tatttttata tttttttatt taaaaagagg gcaaggagct gtgacaaaaa
    48001 tcttgtcaca gctccttgtt ccaaataaac agtgttctcc tgccaaaacc tcggtcataa
    48061 gtcacaatat acataaaaca tgaaaagctg ttttttgaaa ttatcccttg tgactcagag
    48121 ttagacggca ggctcacaac ctctccacta aacgatactt acattgatcc tttgaacaca
    48181 ccgctgtttt tgatgaagta atccacacct gaatagatag taaagatcag acaagcataa
    48241 agcatgattt gatctactgg aataccgatc aggctgaatg ggatattatt gataaacagc
    48301 aagataatag caaccatttg tgtagccgtc ttgacttttc ccggccaagc tgcagccatg
    48361 acttctccat gttcgacaag tagtaaacgc aagcctgtga cggctaattc acgacagaca
    48421 atgatcgcta ctacccaacc aggggccttt ccttggtcaa ccaacatgat aaaagcagtc
    48481 ataactagca ttttatctgc caacgggtcc gcaaacttgc caaaattagt gacaaggcct
    48541 cgcgcacggg caatttttcc atctaaccag tctgtgatac tagctaaagc aaaaataact
    48601 gcacctacta gctgtgtgac agccagtgtg gtgtctccta cgttaaattg tccccaatct
    48661 aaaggtaaac ctacaaccaa aatgaaaatc gggatcatac aaattctaag tacagttaat
    48721 ttatttggta aattcacact atcgactcct ttcttcccta ttctacgtga ctatcagcga
    48781 agtttccacc gatattattt acttttccaa atgaatttat tcaaaggagt atttttattg
    48841 aaattgatct ttttgaatta acgaagaagg accgcgacac agatttgtca cgatccttct
    48901 tttttatacc tacaagtaaa cagatgcgta aactaattta tacgatcatt ctgcgtattg
    48961 aagagtaagg ttcacatttt ttggactctg attgttttcc cctggattaa ctggtacgga
    49021 ttctccattc gctttgatcg ttgcattgct tgctgcacca atcgtgatgg tcgcatttgt
    49081 tgctgtttcc ggtaacacgg tactctgtgt ttcatttgct gccagtgtcc cttgataaac
    49141 ataggcattg tctactagca cacctaacca tacacgattg acagcagtaa attcaagggt
    49201 tacaggcttg gtagcgtctg tgatatttac tgcgatggca ctgccagaat cttctcctgt
    49261 agctattgcc attttctttt cttcacttga ttttgttgtt gattctactg tagaagactc
    49321 tttggtctgc gtgctttgct ctgaggaaga ggactcctcg actgatcctt ctactttaag
    49381 cgaagaagct gtgtctccga ttattggatc agcttgacga tcttgccaag tcatatagcc
    49441 cacgacaacg acaatcgcca aagcaaccaa tcctaataaa atgacaggaa gacttgtcca
    49501 aaagcgactt ggatgcgatt cttcctgatg cattgctttt ctagaagtac ttactgtttc
    49561 aggctgcgga cgttcaggga ccgatcctgt caatttgctt ttcccatcaa aaacatcgac
    49621 taaatggtct ccatcttctc ctacagcttc agcgtattgc cgaataaatg cccgcacgta
    49681 aaatttacca ggcaattggt caaaatcatc attgtcgatt gcttccaaat aacgtttttg
    49741 aatttttgta atctgctgta attcatctaa agatattttt ttattcaggc gtgcttgccg
    49801 taatttttct ccgatggttt caaaagccac tcgttcatct tctccagtct gtttgattct
    49861 ccgaagcatc tgctgttgca acgaaagacg ttatgtttgg gtgctgtgac aaaagtcatg
    49921 tcacagctcc ttttttccga ataaacattg ttctcctgcc aactcctcgg tcacaagtca
    49981 caatatccaa taaatataag aagctgtttt ttgaacttgt tccttgtgac tcggagttaa
    50041 acggcaggct cacgacctct ttcttaaacg gtgttcaatc agctgttcct cggtattagc
    50101 tcaaaaaccg gcaaaatata aggagatatt tttccgtatg tttctctaat actcaggtca
    50161 aaacgctgat ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat
    50221 aagtccaacc aagttaaaaa cattaagaag ctgtttttac ttggctgttc ttattacttg
    50281 atgcagggca gctttttcac aacctcttgg tacacggtgt tccagaagta gcttcttcgg
    50341 caataagccg aaatatcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    50401 ttgaagctga ccacttctgt cacaacctct ttgattactt cgtttgattt tattttaata
    50461 taagttgatt aaaagagagc atgatttgtt ttattttctc actaattttt ccaacacaac
    50521 taagaatacc ataaaagctg attttggagg aggttcctac tacaagtcta aaacgaaatt
    50581 taagactttt cctcataaaa tactggttta ttttggtctc atgtaaaact cgctgaaggc
    50641 ctcttcactt ataaatgctt ccccagctgc cagtacgtct ttcatttgga tcgaatcgat
    50701 gatttcagga agatcaaata aagttcgatc gccaaataaa ctttgcgtaa attgattggc
    50761 aatatattcg atcgaattta atgattggaa atattgtcca agcattttct tcttcagcaa
    50821 atcgagattt gtttctgaca cttccggatc atcagcgaat ccaagaatga tttctttgac
    50881 tttttctgct gctttctctg gttcgtccgt atctccgctg aagtccgcaa agtgaaattc
    50941 acgatccaag ctgaattcaa aaccaaagct gtcatcgata attccttgat tgtacatcgc
    51001 tagatagttt cgagaagtat tgcctaaaat catttggaac aacagattga ttgctgtttt
    51061 atagcgtaac agttctgttc cttcttgcgg caatgtgtct aatcctttaa taccaagtac
    51121 aaatttatct cgtgtaatgg ctgcttccaa tgcactttgt ttgatgattt cttttgtgtt
    51181 ttcagggaaa taacgtacaa tttcttgctt tggagggaaa ttttttgctt cttggttttc
    51241 ccgaataagt ttcatcaatt tctcaggctc catcttacca actacaaata aaaccatatt
    51301 gcttggctga taaaacgtcc gatagcacgt atacaagtct tgagctgtga ttttgtcgat
    51361 actttcaacc gtgcccgcaa tatcgatatg caatgggtgt gtaggataca agttatttag
    51421 tatgccaaaa aacatccgcc agttagggtc gtcttcatac atttggattt cctgaccgat
    51481 gattcctttt tccttgttta cagtttcttc tgtgaagtaa ggtgcttgta caaaatcgat
    51541 cagagtcgtc agattctttt ctacctgatc cgtcgtagaa aacagatagc tggttttcgt
    51601 aaaacttgtg aaagcattgg cggaagctcc ttgtttccca aataattgaa agacatcacc
    51661 atcttctttt tcaaacaact tatgttctaa aaaatgggca attccatctg gaactttcac
    51721 cttttctttt tcaccatagg gaataaattc gttatcgatc gaaccataat tcgtactgaa
    51781 caaaccatat gtcttgtgat agtcattttt aggcaacaaa tacacggtca aaccgtttgg
    51841 cagtacttca tgataaagtg tttcattgat ttgctcatat tctgttttat tcatttgcat
    51901 ttcctcctgc taaaaagaag attgcctgca attcgatttg ctcagctact tgttgtatct
    51961 ctttgatcgt tacgctctcc atacgtttca accattcttc atcagataat tttgtttccg
    52021 gcagccagct gtccaaaaaa gaagcttcga ttgccgcttg cggagagtct agagaaagca
    52081 gatattggtt tctcagcatt gcttttgtct gagacagttc caactctgtt atttcaccat
    52141 ttcgcaaact ttctaattgc tcgtgaatca gatgaagtac ttgatttcgg ttcttttcat
    52201 cgataccggt ttggacactc atgaatcctc ggaacgtatc cacgctacta gaggcataat
    52261 aagctagact ttctttctct ctgacattca tgaaaagttt agagtgagga aaaccgccaa
    52321 ataaaccgtt aaagaccatt agcgcaaaac gttctggttc atcgtaatac acatttgtct
    52381 gataagctag gttcagcttt gcttgaacga ttggctcacg tacttgtcgt tccttcacga
    52441 tattcacttg aggttgggta taaaacagat ctggataaac tgtttcccgc gactcaaatg
    52501 gtaattgccc aactgcttca gctgctttgt ccggatcaac atcgccgaca acaaaaatgt
    52561 cgatttgatc atttgccatc atttcttgat agtaggacgc tagcgaacta gcagtgatct
    52621 tgtccaaatc ttcgactacg cccaaccctg ggatcttttg ttccgggctg ttttcaaaat
    52681 aaagttcttg caaggcatag gaagcaaaag tttgtttatc ttccttcaaa ctttccaaat
    52741 atagacgcag attgttcttt tccaagtcaa acgtcaactg atcaaactga tgatttttaa
    52801 tatttggata aaataaaacc tcttttaaaa aatctaccgc ttgagaaaaa aggtttggat
    52861 cattgacata ttcaccattc acaaaactga tccctgcgtt tagccaatgt aaatttcctt
    52921 tttttctaac tgataatcca aagcttgcgc cataaagctc tgcaagttgc gcacttaact
    52981 ttgtctgatc cgaataattc aaactatttg tttccaacat gctgctgaga agtgaacgtt
    53041 tcgtgatgac ttcttgttgc agacgcgcag taaaacggat aaaaatccgg atcgttttgt
    53101 attgttttgt tggtaagaca tgaagattca cgccttctgc caattgatac accatattta
    53161 tatgctcctt ttcacagttg attgcccgct tactctttta atgggtcaac ctgccttttt
    53221 tacttattcc tctgcttgaa atttataagt tcaggcaatt cgccgttttt tctaaatgat
    53281 gttacacttt atagtatagc atagatgttt tttatcaaaa gaaacagacg gctaaaagcg
    53341 tatattgttt ttttattttt tatttttcga tatactaaag acaaataaaa gaagggaggg
    53401 tcattcatga tcaaagaatt caaagagttc atcatgcgtg gagatgtctt ggatcttgct
    53461 gtcggcgttg ttattggtag tgcgtttaca agcatcgtaa accaagtggt gcaaggcttg
    53521 atcacaccac taataggttg gtttgtcgca atgatcacag gtacttctga tttagaagga
    53581 gcactttcaa ttcttgactg gtcgccgaca aaaggcgtca catttgcgtt tggtaacgtt
    53641 attagtgcta tcattacatt tctgatcact ggttttgtct tattcttagt tgtcaaagcg
    53701 gcaaaccgtg cgaagaattt acgctccaaa gaagagcaag tggaagtgga agaaactcct
    53761 actgcagaag attatcttag tgacatacgc gatcttcttg ccaaacaagt ggcagaagac
    53821 aatacacatc cagtagagca aacaaatctt tccgatactt ctaaataata aagaatctaa
    53881 aaaaaaagaa agccgatttt cagtgtcggc tttctttttt tagattctta acttgctact
    53941 tgttcctttt tcatgtcgat atgtggaata tcatcttcca aatacacatc tgagattgcc
    54001 ttgaatccaa acgactcata aaaagcttgt aggtattctt gtgcgccgat cacgattggt
    54061 ttttctggaa aatttttctg gatcatgtta atcgtttctt gaacaatctt tcttcctaaa
    54121 ccatctttac ggttgtcctg atgaacaagg acacgtccaa aatggatact ctccgcttct
    54181 tcatatactc gcgtgtaggc aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa
    54241 gcttctccat caatgtcatc aacttcttga taaggacact gttgttccac aacaaaaact
    54301 gcgattcgtt ctttgacgat cgcgtaaaac tctgctgttg acaattcttc aaatttcttt
    54361 acctgatatt ccactaagca aacgccccct gcttttttat tcttagcata ccgattcgca
    54421 cacaaaaaag caaacgaacc ttacgaatct caggctcgtt tgctttttaa actgcttcag
    54481 ttaaaaattc gatctcaact gtccgagtaa gtacatctga atagctgtaa gatactcgtt
    54541 caaaagaatt ttcttcagga tccaaatcaa caacgaatac tgaaggatag gtttctgtta
    54601 aaacgccgcg gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac atgattgggc
    54661 tgccgatacg accttctaaa tctttcttga ttgaagctaa agttgttggc atacagttca
    54721 cctctactat caactatacc acatattttt aaaattttca agtttaccat aaaaaaaaaa
    54781 gaaatgcaat aaaaataaat aagaagctca ttaagatttt tctaaattat tcctcattat
    54841 aaaatttcaa gtgttttttt attttgagtt ttactctccc gtaagcgctc attacttgct
    54901 tgtgatccat tgctaaacat tcagccatgt ctacaaaatc aacgccttgc atataataat
    54961 gaaaaatttt agcttctatt tttgaaaact ggatttcgca attttcgatt gcttctttta
    55021 ataaaagatg ctgaattgct aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc
    55081 cttctgtctc tattttttgg tcgattgaga cttcatcttt ttgatcacga cgcttcagcg
    55141 cgttctgctt cctcaacaga gaatagattt gattgcttat aacgctttta taataacttc
    55201 caaagctaac acctttgctt ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg
    55261 cttcttgttc ccaatcctca gaaacataat ttcttaaata ataggtgttt ttcaaacgta
    55321 gtaaaagtgg aaaatatttt tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg
    55381 cagcgcgcaa taactcctca gacataaaat aattcctcca taaattcgtt tatggaggaa
    55441 tcttatcatc aatgattcat cgaactgatt agaacgatat gttatttatt ttttctcatt
    55501 aaatcgtcca atttttggga caattttttt aactgctctt cattccatgg agaattacgt
    55561 cggaaatcaa taaaatgcat atctttattt tcttgggcta ttgttttttc cgtttttttt
    55621 atcgtcttat ataattcata agcagaagtt cgtaatgctc cttgggaaaa gatcatccac
    55681 tgttctgcta aatcgcttgt cgccactgtc acttgtgtca aacggtcatt cagctgacca
    55741 gcgattctct cgatataact gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac
    55801 tggtatttct tataattttg ttggatgccc ggaactagtt gggcatcgaa gacaacgatg
    55861 acttccattc cttcatattt cgcataattt gataaacgat gtaaaagagc ttctcttgca
    55921 tccgccattt tgtcctgctt tttaagctgg acaagttccg gccacgaacc aatcatgttg
    55981 tagccgtcta caatcaacag ctgttttttc atggtctact ccccctgacg ttgggaaaaa
    56041 gcctgataca taagcaaacc agcagcaaca ctggcattta agctctggac gtgcccggtc
    56101 atcgggatcg tcagcaattc atccatttct tttttcaatc ctggactcat tcctttgcct
    56161 tcattaccaa tgactaaagc aattgatcct ttggcattcc atttttggaa gtttgtgccg
    56221 gtcatatctg tgccaaatac ccaaaatcct gtttctttga gctgttgcac agcttgtacc
    56281 aaattcgtca cgcgtgcaac agggacatgt tcgactgccc cagtggaagt ctttgttacg
    56341 ataggtgtga ttccaactgc tcggtgtttt ggtatgatca caccatcgac gccacttgcg
    56401 tctgctgtcc gtaaaatcga cccaaaattg tgtggatctt cgatgctatc caaaatcaaa
    56461 aagaaggggt cttctttttt ctcttttgtt cgttcaatca gttggtcaag ggataaatat
    56521 tcataaggtg taatcgcaag aacgactcct tgatgcacgc cgtgatcgct caaagtttct
    56581 aatttttgtt taggaaccca tttgactggc acagcttgtt cacgtgcgat ttctttcagt
    56641 tcttctactt tttctcctcg tgaatcttct tgaagaaaga gtttattccc tcgcccttgt
    56701 tgtagcgctt ctactgcggc atggtgccca aagacgaaat tatcttctaa tgcttcttca
    56761 ttttgatcag aaactgactg atttcgtcca gtttgctttt tatttgaata tctattttgt
    56821 tttttgtctt caaatccacg tttcgattga tgtttgtttg ttctttcttt acccattttt
    56881 ttcaccaact ttctgaatac accagcggat caattcttcc atccgctcgg tttgttttgt
    56941 caaatgcaga tagcccatga gcgcttcgaa ccctgttgcg acccgatacg tcgtaacatc
    57001 agcatttttt gcgctcgtat ggcttttagc atttcttcct ctgcgataat aaagttcttc
    57061 ttcttctgtc aaaagatgtt cttccagcat ttgctgaatc aaaaaggctt gtgcattcgc
    57121 agaaacataa ttcgttgccg cacgatgcag cttattgggt tttgtttctc cttgatcaat
    57181 caggtggtca cgaacataga tttcgtaaat cgcatctcca acataagcca aggctaaacc
    57241 gttcaattgt gtgtaatctc tcatgcttct cttctccatc gggttccttg cggtgtatct
    57301 tctaataaga tccctttttc tttcaacaaa tcacgaattt cgtcgcttcg gacaaaatca
    57361 cgattgcttc ttgcttgatt tcgttcttcg atcaattgtt caatctcttc atctaataac
    57421 tcttctgctg taaataaaat accgaagatc gaaagccatt gcgagaataa agcttctcct
    57481 tgtgccaaga cagcggcaga aactttcggc tggtctagat aagtgttcag ccacttagca
    57541 agctcataga ccactgtgat cccatttgct gcattaaaat catcgtccat ttcttcgatg
    57601 aatcgctgtt ctaaattttc taacaaagcg attttatccg catcatctgc caaattctct
    57661 gaggcagttt cttggcggaa tttcaaattg tcaaacgtat tcttcagtcg ttgtaaattg
    57721 gtggctgctt ctttcatcgt cgtttcactg tagcggatcg gacgacggta ttgtgtggtc
    57781 gccatgaaga aacgcaagac ttgtggatca acgtttttga tcatttcatg tacagtgatg
    57841 aaattcccta gagatttact cattttttca tcgtcttcgc caatcgttac atagccattg
    57901 tgcatccagt aattagcgaa ttttttccct gttttcgctt cactttgtgc gatttcattt
    57961 tcatgatgag gaaattctaa atcttgcccg ccaccatgga tatcgatcgt ttctcctaaa
    58021 tgttttgttg ccataacgga acattcaata tgccaaccgg gacgtccttt gccccatggt
    58081 gaatcccaag aaatctcgcc aggttttgct tgtttccata aagcgaaatc taatgggtct
    58141 tctttttgct gctgttcttc ccctgtacgc tgacttgctc cgacttccag ttcatcgatg
    58201 gattggtcac ttaatttccc ataattttca aatttacggg tacggtaata cacatcacct
    58261 gaagattcat aagcatagcc tttttcaacc aacacttgaa taaatgctaa aatatctggc
    58321 atatgatcca tgactcttgg atgcagtgta gcaggttcga cattcaacgc tttcgtatct
    58381 tcttcaaatg ctcggatgaa acgatcagca acttctggtg ctgtaatacc taattcgttt
    58441 gctgtacgga tgattttatc gtctacatcc gtgaaattcg atacgaaatc gactttatag
    58501 cctcgatatt caaaatagcg tcgaattgta tcgaaagcaa tggtgctacg agcatttccg
    58561 atatggatat aattatacac agtcggtccg cagacataca taccgacatg tcccttttgt
    58621 atagggatga attcttcttt ttttctggtc aatgtattgt agatttttat cattctgatc
    58681 tcctcacttt ttctcctttg attcgaacga cttttgctgg tactcctacc gctgtagcgt
    58741 cgtctggaac atctttcaac acaacggcac ctgcaccgat tttggcgttt tttccgatcg
    58801 ttaccggtcc taatatctgc gcattcgcag agagcatggc tccttgttta acagttgggt
    58861 gtcttttacc cgtttctttt cctgtgccgc ctaatgttac gccatggaat aaaatcacat
    58921 cgtcctcgat ttctgcggtt tcgccaatga cgattcccat tccatggtcg atgaacacgc
    58981 cagttccaat cgttgctcct ggatggatct caacaccagt taggaaacgc cagaattgag
    59041 cattgatctt cgccaataaa aagagccggt gctgatataa aaaatgagag aaacgatgcc
    59101 aaaacaaggc atgaaaacca gggtatgtca aaacgatctc agctgtcgaa cgtgcagccg
    59161 gatcgtttct ctttgctgca tcaatcgctc gggtaaacca actcatgact ttccactcct
    59221 ttcgtaagaa ttttatgtag gaaatcatct tcttataaag aaaagaagcg ccttttaaat
    59281 aagtttcctt atcaaaagac gctttccgcg tggttccact tttcttcata atcaatgatt
    59341 atctctgaca gctaacgcct gttatacgtt cttggctact ttgtttccta taaaacagtt
    59401 tttcctgttt tctgtgtttc gccaagacca ctcccagagg catttcggca accattcatt
    59461 gataccttgc accacccggt atctctcttg taatgatcag ctgcttactt ttttctgttc
    59521 atcgtgttta gttcagtact tgatttaaat gtgctaatga tttttcacga ccaagcaatt
    59581 cgatcgtttc aggcaattct ggaccatgca tttgtcctga tacagctaca cggattggca
    59641 tgaaaagatt ttttcctttt acgccagttt ctttttgaac agctttgatg gcagctttga
    59701 cagttggagc atctactact tccatttgtt ctagctgtgc tttgaatgcg tggagaacag
    59761 taggaactgt ttcaccagca agtacttctt ttgctgcttc attcaataca ggatgttcat
    59821 tgaaaaatag cgtagacaga tcaacgattt ctgcagcata gctcatttga ggttgataca
    59881 ggctaacgat cttcttcacc cattcgattg tcttttcatc tgggttttct gctacacgtc
    59941 catcagcgat tagataaggc agacacatgt cagtaaatgc atctagatcc attgctttga
    60001 tgtaatggtt gtttacccat tgcagttttt tcgcatcgaa agctgcgggt gatttactta
    60061 ggcgttttgg atcgaacatt ttgatcaatt cttggtgttc gaagatttcg tcttcgccca
    60121 caggtgacca gcccaataat gcaataaagt tgaacattgc atctggtaga tagcctaatt
    60181 cgcgatattg ttcgataaat tgcaagattg attcgtcacg tttgcttagt tttttacctg
    60241 tttcactgtt gatgatcaat gtcatatgtc cgaaggttgg tggtgtccag ccaaatgctt
    60301 catagatcat cagttgtttt ggtgtattgg caatatggtc atctccacgt aaaacgtgag
    60361 tgattttcat caaatgatca tcgaccgcaa cagcaaaatt ataagttggc ataccatcgc
    60421 gtttttggat gacaaagtcg ccaccgatat tatcggattc gaacgaaata gcgcctttca
    60481 ccatatctgt aaatgcgtat tctgtattgc gaggcacacg gaaacgtaca acagattcta
    60541 gaccttgtgc ttctttttct gcttgttctt caggtgtcaa gttcgcacat gttcctgcgt
    60601 aatgaggcat ttctcctctt gcgcgttgtg cttcccgttc tgcttctaat tcttcttctg
    60661 tgcaatagca tttgtatgct cgattgctag caagcagctg atcaatcagc ggttgataga
    60721 tttctttacg ttcagattga cggtaagggc cgtattctcc aggattttca ggagattcat
    60781 cccaattcat tcccaaccaa gcaaggtttt ccaactggct tttttcgcca tcttcgatat
    60841 tacgtttttg gtcagtatcc tcaatacgaa taataaagtc tccatcattg tggcgggcaa
    60901 acaaataatt gaataatgca gttcttgcat ttccaatatg caggtgccca gttggacttg
    60961 gtgcatagcg tacgcgtact ttcgtcattt tcttcctctt ctttcaaact agactttgac
    61021 taacagcaaa aattccgtca gttacggctt ctcaaatatc atttatttaa agccactcag
    61081 cacaaatttt accttttcta aggtaaatag taaagccaac tggttgaatt tccttaccag
    61141 ttggctgaat ttctttttta ttttgattgt ttagtctttt tctctgtctc ttttttattg
    61201 tcgattcctc gtcctgcatg tgcaggttta gcaaagatca tgcgtccagc agctgtttga
    61261 agggcacttg ttacaacgac ttcgatacgt tcattcatgt aatgctgtcc atcttctacg
    61321 acaaccatcg tgccgtcatc aaggtaggct actccttgtt ggcgttctgt cccggctttt
    61381 actactagaa cattcatcgt ttcaccaggg atgacgactg gttttacggc atttgccaac
    61441 gcattgatat taagaacagg tacattttgg aattcagaga ctttattcaa gttatagtca
    61501 ttggttacaa tgacaccatc taaaagctta gccaatttga tcaatttgct gtctacttca
    61561 ctgatatctt caaagtctcc atcgtacatt tcgacagata taccctcttc tttttgcaag
    61621 gcatttagga tatctaatcc gcgacgacca cgcacacgtt tcaaactatc ccctgagtcc
    61681 gcgatgtatt gtagttcgta aagaacaaaa ttagggatca tcaaggttcc ttctaagaat
    61741 ccagtctttg taatatcata aatccgaccg tcaatgatca cgcttgtatc caagatttta
    61801 tatttatgga aattttctcc tgattttcgt tctaacagtt gactctcaac ttgactagct
    61861 gtttcttgct cgattttttt gcttcgtgaa ccaaaaactt tcttccattc ttcgattcgt
    61921 gtcgttccca ttctcaatcc cagatagcct aagatgatca tcagtaaaat cggcaataca
    61981 ctattgacga atggaatttc catattgtaa agaggggtcg agatcacaac acctaaagtc
    62041 aatccgatga tcgcaccgat cgcaccgaag agaagataag tcaagctgac ctcacttagt
    62101 ttttgttcta cttgtttgat aatagctgag atatgtttgg ccaacagcaa agataataaa
    62161 aagaaaataa gtgcgcctaa caggctgttg gtaaatgtat tattcagcca tccgtttgac
    62221 tgttgaccga ttgcctgcca agctgagggt aaaaatgaaa tacccaagct tgcgccgaca
    62281 atgatcatca gcaaagtaat gacacgtttt tgcatcgttt ttcctccgtt tcgacaatca
    62341 tttattagtt agcggaacac acgcttcaac gtttcagcca aagttgctac tccaactatc
    62401 tcgattcctt ttggcgcttc ccagttacct aagttgtttt taggtaggta tacttttgtg
    62461 aagccgagtt tttggacttc tttgactcgt tgttcaatat gactgacacg tctgatttct
    62521 ccagtcagtc caatttcacc gatgaaacat tccgttggct gtgttccttt ttctttatag
    62581 cttgaagcaa tactgacagc gatagctaaa tcaatcgctg gttcatttaa ttttacccca
    62641 cctgcagctt ttaagtaagc atcttgattc tgaagaagca acccagctct tttttccagc
    62701 actgccatga tcagagatac ccgattgaaa tcaagacctg ttgttgttcg tttggcattt
    62761 ccgaacatag taggtgtgac taatgcttgg atctctacca aaattggacg tgtcccttcc
    62821 attgccacaa cgatagcaga gccagttgca ccgtctaggc gttcttctaa gaaaatctgt
    62881 gaaggattag cgacttcttc caatccatgc tcatgcattt caaaaatccc aatctcattc
    62941 gttgagccaa atcggttttt tactgctcgc aaaatccgaa aactatgatg ttgttcacct
    63001 tcaaaatata acaccgtatc caccatatgt tctaacattc ttgggccggc gatcgagcct
    63061 tctttggtaa catgaccgac gataaaaatg gctatcccat ttgttttagc ggttttgagt
    63121 agttctgcag tggtttctct tacttggctg acacttcctg ccacacttgt gatgtctggc
    63181 tgcgtcatcg tttggatgga atcaatgata acataatcag gggagatatg ttcgatggca
    63241 cgactgattt cgttcatatc tgtttcagcg tatagataaa attctgtgtt gatacttccg
    63301 aggcgttccg cgcgtaattt tatctgctct gcgctttctt cgccggatac atataagact
    63361 tttccgccta tggctgctaa ttgctgagaa acttgtaaca gcaaagtgga tttcccgata
    63421 cctggatcgc caccaatcaa gaccatagac cctggcacca cacctccgcc taacacacgg
    63481 ttcagttctt ctaattttgt tttgatccga ggttcttttt tagggacgac gtctgcgatt
    63541 tttgttggtt ttgctttttc tcctgtcaaa cttgtccgag ttctgcggtc cgttgtatct
    63601 tgttcgattt cttcgaccat cgtattccat tttccgcaat ttgggcagcg tcccaagaac
    63661 ttaggcgaca catacccgca attttggcaa acaaattgta cttttgattt ttttgccatt
    63721 gtaccgcctc cttttatcct aatcatgaga cattaaatca agcttaagta tctttaacgt
    63781 tttatttact ggaagaacca aatccgcctt gacgttgagc agtcgtttga tcttgatctg
    63841 cttttaaaaa cggcaaaaag atcccttggc cgatacgctc gccttttttg atcactacat
    63901 ccttgaaacc aaagttggtg aattggaaca tgatatggcc ttcatttgcc tcattattgt
    63961 aataatcact gtcaatcaca cctactccgt ttgtcaacac taaaaaccgc ttcaacggat
    64021 tgcttgaacg gttagcaagc tgcaaaaatt catcttctcc catgtaagct ttgattccag
    64081 taggcacgag ggttggtttc aattgttttt cattttctga tacatctttt ttcaatccat
    64141 ctgcgactgt ttccaacccc tttttccagc ctgctttcca gatacttgga atcacgacct
    64201 cttctgctgc ttcaaaatca tagccagcag catgatgtgt agcgcgctgc ggtagattga
    64261 tattttgatc tttatattta gaaataactt caaatcctct ttttctcacc ggttttcctc
    64321 cctaaataaa ctactcctat tctaacatat ccgaggctgt aaaagacttt ctttacaaat
    64381 tcttcaagaa attctgctat cttaaagtaa accattaatt cgttcttttt ccaaacgact
    64441 gcaagttgta caaaaaaact attttttaca tgactttact cgtcaaaaca agtagaatag
    64501 aacgaaatta atgagtggat atttgattga ggtgtcacat gcgtaaagaa attaaaaatg
    64561 caaaacgaat tattgtcaaa gtaggaacaa gtacattgat ttatcccaat ggaaatatca
    64621 atcttgcggc aatcgatcag ttagcttttg tcctttctga tcttagaaat cggggaaagg
    64681 aagtgatctt agtctcatca ggagctgtag gcgttggtat gcataaatta catttgtcag
    64741 aacgtccgac tttgattcct gaacagcaag ctgcggcggc tgttggtcaa gccgaactga
    64801 tgaatatcta taatcaaaga ttcagtgtat ataatcaaca gacggcacag atcctcgtga
    64861 caagagacgt ggtagattat cctgaaagtc gtaaaaatgt gaggaataca ttggaacagc
    64921 ttttgattat ggacacgatc cctatcgtga acgaaaacga tactgtagca gtggatgaat
    64981 tagatcatct gacaaaattt ggcgataatg atcagctgtc agccatcatg gcaaaacttg
    65041 tcgcggcaga ccttttgatc gtcctttctg atatcgacgg gttttattca gataatccga
    65101 ctacaaatcc cgaggctcag atgtatcgct atatcgataa aatcgatgaa aagattctgg
    65161 cacaagcagg tggtgctggt agcacatttg gaacaggcgg tatgcagagt aaattaaaag
    65221 ccgcttctag gatatttgaa gcaaacagcg caatgatttt agcaaatgga aaaaatccgg
    65281 caattatttt tgatattctt gcaggaaaag aagtaggtac tttatttaag gaggacttga
    65341 aatgactgac ttgatgcaat taggacaaca ggcaaaagat agcgcgcaac aactagcact
    65401 tatgcctaca aaccgcaaaa atgagctact tgttcaaatg gcacaaacca tcaaaaagaa
    65461 tcaacaagcg atcattgaag caaatcataa agacttagaa aaagcagtcg aaaacaatat
    65521 cagtgaaacg atgcaagacc gcttgcagct gacacctgag cgaatcaatg ccatggctac
    65581 agagatcgaa aaaattgctt cgttagacga tcctattgga aaagtagatg agatgtggac
    65641 gaacaccgac ggattgagaa tcggcaaaaa gcgcgtgcca ctaggcgtga tcggcatcat
    65701 ttacgaatct cgtccaaatg tcacgacaga tgcagctagt ctagcattca aatcagggaa
    65761 tgccgttatc cttcgcggtg gcaaagaagc attcttctcg aatcaattat tagtccaatt
    65821 attgcagcaa gtactggtca gtgctggcga atctccttat gccatccagt ttgtcgatga
    65881 tacttctcat gaaactgcag gacagttgat gcgattgaca gaatacttag atgtgctgat
    65941 tcctcgaggc ggagcaaaat taatccaacg agtcaaagaa caggcaacgg tacctatcat
    66001 tgaaacaggt acaggaaata atcacgtata tatcgataaa gaagcacagt tatctatggc
    66061 agtgaacatc atcgtcaatg caaaagcttc ccgtccatct gtctgtaacg cggcagaaac
    66121 attgctgatc cattctgaga tcgcaccttt tttcttgcca gctattgaaa aagaactggt
    66181 agaacacggg gtctctttac gtgcggatgc tcgtgcgtta gaatatctag agacggctgc
    66241 tttagcagaa gacacagatt gggatactga atatctagat tatatccttg ctgtcaaagt
    66301 ggtcgattct ttggatgaag caattgcaca tatcaatcgg cataatacta aacattcaga
    66361 aacgatcgtg acagatagtt atgcggctag tcaacgtttc ttaaatgaag tggacgcggc
    66421 ggctgtctat gtcaatgcct ccactcgttt tacagacgga tcggtatttg gttttggtgc
    66481 agagattggt atctctactc aaaagttgca tgcccgtggt ccaatggggt taaatgaatt
    66541 aacttccaca aaatatatca tttatggcga tggacaaatc cgcgaataat gtttcgtgtg
    66601 aaacattatt cccgcctttt gccttttttc tgtaaaggtt ctatccaact agaaaggtgt
    66661 caatatgttt gaaattttaa tgattttttt tatttacctt atttctctga atatcgctgc
    66721 ttttctggga gtcggtattt tatccctttt ctttcaattc aaaaaacgaa gcatagggag
    66781 tcaacgggaa aaatggtcgc agtattttga caaaatcggg caaaaagggc tggttacacg
    66841 cttgcacatt agttacatgg ttgcattatg cttattagcg acaatcaatt actattcttt
    66901 tttcgatcat tcaatcgctt atacgattac gctactgatt gctgggattt ttcacttatc
    66961 ttataaatat cagctgaata aaaaccactt gaatcggact ttccgctaaa aaatatccgt
    67021 acgataagta gagtgccttg aagatcggca aaaaactgcc ggacttttta gaggctgtga
    67081 caaagatttt gtcacagcct ctttttccat ataaacggcg ttcgcctacc agctgcctgg
    67141 taaatctgtt tcctcttcgt taagaagcga aaatccgtca aatgtttcgc catcataata
    67201 acgaagcagc tcatccatgg acaagttcat cgttttttct ttttcttctt ccgctgattc
    67261 agccagtagc tgttcccgat acacctgata cataccagct attttttgct gcatcgcttt
    67321 tctagtttca ccttccgcat tgattattgg caattctttg atatagccac gataaatcaa
    67381 ccgatcatct gaaaattcaa tgggtaattc aacgagtgtc aacccgactt cctgaaaata
    67441 ttcttccagc atctattatc cccccattct ttttcttaaa tgaattttag cttacctact
    67501 gaaaattttc cagtttcatt attttgagaa aaaaagggaa tgtgacaaaa atcgtgtcac
    67561 atcccctttt tccgaataaa tggtgttctt ctgccaactc ctcaatcata agtcacaata
    67621 ttcaacaaac atgagaaact gttttttgaa attgttcctt gtgattcaga gttaaacgac
    67681 agtctcacaa cctcttggta aacggcgttc aatcagctga ccctcggtat tagctcaaaa
    67741 aacggtaaaa tatgagaagc tatttttccg ttttgtttct ctaatactta ggataaaacg
    67801 ctgattttac aacctctgtt aaacggtgtt caatcagctg tccttcggta ttagctcaaa
    67861 aaacggcaaa atatgaggaa ctatttttcc gtttgtttct ctaatactca ggacaaaacg
    67921 ctgattttac aacctcctga taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    67981 aaccaaacga aaaatatgag aagctatttt tgtttggctg ttcttatgac ttgatgcagg
    68041 acagcttttt cacaacctct gatttcttat tcttcttcat ttgtagcaaa ataattacgg
    68101 tgataaatga tacaacatac actgataaga acaatggcac ctgcaaaata aaacgcatag
    68161 ctttcaccaa tatgttcacc aatcacacct gcaataaatg gtgctaaagc tccaccgaaa
    68221 aagcggacaa aactatagct ggacgaagca acgtttcgtt gcagacctgg tatttccata
    68281 gcaatcgtcg tcaacagtgt attgataatt ccttggaaaa atcctgctaa cacaatgctg
    68341 atcggtacaa ggactggcat agaagttcca gctcctaata acagcaaaca aataatgaat
    68401 aagacaaatg ctgctaaaat tgtccgataa gtcgtaaact tccgctcaag agccggtgca
    68461 acgaaaatcg aagcaatagc cagaagcaca ccccagccaa aaaatacaaa accgacttct
    68521 aattcagaat agccaatcaa taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa
    68581 ttatataaca atgcgattag tccaactgaa cgaagtttat gattgcttaa cgctttgaca
    68641 ccttcccaaa ggcctacttt tctctttggt ttctcagttt caggtaaaag taccgctagg
    68701 gctataaaac cgcaaaacat caaaaatgct accccaaaaa atggataacg ccatgaacca
    68761 ctgcccaaga ccccaccgac tagagggcca acagccattc cgcagccaag tgctgcttca
    68821 tacatcatga ttgctttttc tgtgttacca actaaaatac tgacgatcgt tgctaaagca
    68881 gtagagacaa ataaagcatt cccgacaccc cagccggcac gtaacccaat cagcattccg
    68941 atattcccag ataatccgcc aagaccagcg aatacaacga tgataaacaa tcctgtcatc
    69001 aatgtttttt tcaaaccgat ccgactagat aagaaacctg taaatagcat caccacacca
    69061 gtgactaaca tgtaactagt aaataacagg gtcgtttcag ctggcgtagc gtttaactgt
    69121 gcagcaatcg tttttaagat tgggtctact agacccactc ccataaaagc gatcatacag
    69181 gtagctgcta caactagcgc tgaacttttc ccctttgttt cttcatttgt ttgttcttgt
    69241 atatctattt gattctccaa tatttccatc cctttctctc tattagcatc atacatgatt
    69301 tattttacat gtcaatatgc acatgtaaaa cgtggaaact ttactgtgat ataataaaga
    69361 aaataagacg agaggtagga tttttatgaa tactttagcc tatatattat tatgcgtgct
    69421 tttatccaaa ccacgttctg ggtatgaatt gaagcagctg atcacaattt tttgggaagc
    69481 ccatcacagt cagatctata caacgcttgc caagttagac cagcagggtt atgtgaccgt
    69541 tttggacaaa gacgagcact ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc
    69601 tcttgtagag gaatggatca aagaggaaac tcccgctcct acgcaaaaag atgaatttct
    69661 agcaaaaatc tatgcttttt cgacgctcga taaaacaaca gctactggac tgctattcac
    69721 tagagaacaa cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga atgacctctc
    69781 gctagacaag aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg
    69841 tcagcaggaa ctccaatggt gtcagcaagt aagagaacaa atgcagcagt tttttgagta
    69901 agggataaac agaacctttc tagaaatatc tattgaagag caaattatct tcaataaaag
    69961 agcaatacag ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct atcgttcgga
    70021 tcactatttt tctagaactg cattcaagag actatgacaa aaagcatgcc aaggcctttt
    70081 ttcaaataac agtgttccat cattcattac tttgaattgc ttttagcaca cttcttgaaa
    70141 gcgggccaac gatgatcaac tgcaacggta aagcggcaat cacattcatt ttccaagcat
    70201 tcatataagc agagaataat tgatcaggta ttccttgttc catgatcaca ccgaataaag
    70261 acatacatgt caccattcct aaaaccatca agcatgaaat agttaagatc atttggacct
    70321 tgctttctgg atttactggt agtttaaagg caattttttt cgccagcaca ccgatgatga
    70381 agacatccaa aatgaaagcc accacgaatc caggcactag accaatcaac aaacttgata
    70441 aatgaagatc ttgatgcagc cataaattat acgcgctcat ccccaagacc atcaaaaaac
    70501 acatcattgt tgtaaaaatt attccttctt ttttatttgt aggcaatctg taccactcct
    70561 ttaaatttgt cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag tacttttttt
    70621 attttctgcc cttttatttc ataaacttca tcagccgtac gctcgacaaa gtaagaatca
    70681 tgagaagtaa acaaaatcgt tcccggatat atttgcatta gcttctctaa agcttcgatc
    70741 gttgccatat caataaaatt agtcggttca tctaacagca acacattact tggcttagta
    70801 aaaagcaaag caatcgtcag acgggtcgct tctcccccac ttaatgtaca taacggtttc
    70861 gtcaaagttt cctgagcaaa ccctaagtga ttcagcaaac tacggacaag cgattctgaa
    70921 aactctgttt gtctcatgac aaaacgaatg atggattctt cagatgtcat ttgataaccc
    70981 ttttgctgat atacttgaaa gcttactttc ggagagagaa ggattccttc tccttgtttg
    71041 cggatatgtt ctaataaggt tgtcttacct gaaccatttt caccgacaat cgcgattttt
    71101 ttacctaaag aaaactgaaa atcaccattt accagtaatg ttctcccact tctttccaat
    71161 tggacatttt gtcccatgat gggatagcgg ctgtggagtt caagagattt tggtactgga
    71221 aaacggattt gtttcatctg ctgcggcttg gttgtttctc ctattttttg gagtcttctc
    71281 tctaatgttt tcgcattctt ttggatggct ttttgtacac tgtctttttg tttagaggaa
    71341 gacaaccgat ccagtctgat ttgttgcttt ttctttttac tggacacttt ttccaacttt
    71401 tccgcttgtt ttttcttttc ttggatcgat ttttccaatc gttttttctc tttcatgatc
    71461 gactctgctt ctcgtagctg agtctttttc tccaattcct tttgagtgag ataggcacta
    71521 taattccccg aaaactctcg gatttctccg tcttttactt cccagatttt cgatgctagc
    71581 tcatctagaa aatagcgatc atgactaaca aaaaccagcg taccataata ataacgaagc
    71641 tcttcaatca gtcgatcaat actttttcta tctagatggg ttgttggctc gtctagaagc
    71701 atcccttgtt cataagaaga aagaagacgt atcaactcga tctttcgttc ttcccctccg
    71761 ctccatttcc gctctgaagt ttcttccagt cttaactgga aatgactggc taattccaaa
    71821 tttaggtcct ttgcttcagc gagataggtt aattgaggca ggtagttgaa ggtgatctct
    71881 gtttggattt tcccatgatc gggaaaaagt tcaccggcta ttagtttgag caaagtcgat
    71941 ttaccgacac cattttctcc tatgatccct atacgatctt gctgatagac acgaagagaa
    72001 tcaatagata gttctagctg gttgccaaag ctgacttgta gatttgttat atttactgct
    72061 aaattttcca tatgttttac actcctattt catgtaaaac aacaattatc gatactaaaa
    72121 aagagcagaa aaatctgttt gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa
    72181 ataactatga ctaaatcacc aaatctgtca tagctttcac ctattctact gtcgttcgaa
    72241 aaaatggaca tactccatcc atgaaattgt tgtttataca tgtcttttga atttatttag
    72301 ttgttattca aaagttcgaa acgtaatttc atcgatgcag tcatttttcc gacctccttt
    72361 tttattaggt taccacacac gattcaaaaa aagcaatagc tactgaatag taactgaaaa
    72421 ccttttattc gaacatagtg atcttttctg gtgctaaacg aacagactcg taaccttctt
    72481 ctaccgcttt tcctattgat aaaataataa ctggaacata acggtcttta tccaaaccaa
    72541 atgcctctgc caactgatct gcttcgaatc caccaattgg atttgtttca tagccatgat
    72601 cacgcgcaac taacatgaat tgcatagccg ctaagctaga atcgactttg actacatcgt
    72661 tcatttcttc tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat tgttgatctc
    72721 tgacttcttt tggcatcttc cctgattcgt aagcttggtt gtagatttct tcgccatatt
    72781 cgtaacattg catatcgcca aaaatcatga ccattgcaga agaagtatca ttttgtctcg
    72841 tattgaaacg gatcaaagga cgcaagattt cttttgcttc ctcgctttca gcgaccacaa
    72901 agcgccatgg tttcatattg acagaagaag gtgcagtcgt cgcttctttg atcatttcca
    72961 gcatttcttc gtgtgggatt ttgtatgttt catcatatac gcgaacggat cttctaccag
    73021 tcataattgt tgaaaaatca tttttttgtt ccattattat taacctcttt cgtttctgtt
    73081 gttatattgc aaagttgggt attcaattta tctaaaagtg ttagaaattg tttcatttct
    73141 acatcgctga aagccgggga tagcacggaa cctgtatctt tatggtccat ttcacaatgc
    73201 ttgattttct tctgggcttt ttctgttagc gctacataca cttcccggtt gttctgagga
    73261 tttctctgac gcgaaacata gccatttgct tccaatacct tcagatgacg agtaactgct
    73321 ccttgatcaa tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc
    73381 agtctcatca tgatctcgta tctagtaaga cttataccga tattttcttc aaagatctgg
    73441 acgatttttt gatcggctaa tttgatttga tataaaaggt gactaatctc ttctaaagac
    73501 ataatttcct cctttctaat aattgactcg tcaacgattg acttgtcaat aatagaataa
    73561 aaaaagaaaa aagtcaaaga atcagctgtg taataaaaaa aacgctctca atagaaaagt
    73621 atagagaaaa gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct
    73681 aaaaccaaga ttattgattc ttatcaaact atagaagata gctcttctgc agtagataat
    73741 tttacaccgc caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa
    73801 ttggatcagt atgcttaaac agtaataaaa aagttatgac aagatgttgt cacaaccttt
    73861 tttgagtaaa cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa
    73921 catgaaaatc tgtttttgtt tggctgttct tactacttga tgcagggcag ctttttcact
    73981 acctcttttt aataaccata aaatcctatt ccttcatatc tccagcccat cggtgttagc
    74041 caatctcttt ccttagcact caaggtatag tgatgggtac cagttttttc atttggatta
    74101 aatagtcgat aaattggctt ggaaccacca gaataaaaac ttagaccttc ataacgccaa
    74161 ccgatttttg ttaaagagtt gatctcagat tgtgccattg tgtaatgatg gtctccactg
    74221 taaggattgt ataaacggta gacaggttgt ccgctgtttg gtgcttgcca agcaatccct
    74281 tcatattccc agcctacagt tggcagataa tttttttctc cggcattcaa tgtatataaa
    74341 tgttctcctg tatgacggtt atacagacga tacatcggag aagttgaagt tggtgctgta
    74401 tcatactgtg tcagattata ggtttcgatt acattgttca atgtagaacc ataattaggc
    74461 gcagtggcat aacgacctgt tagccaagcc gtagcatctt tgtaagagtt cgtattgctt
    74521 ttccatgcac cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt
    74581 acgttatctt gcaatgattc tttgtacgaa ggatattttc taaaagcggc attcaccgta
    74641 atccattgcc cattcacata ttccgaagta ggcatcgtaa cagattggcc attatagctt
    74701 cctttaatcc caaatagatt gtaattaggg gcagaagcca aagtactgtt cccccagcca
    74761 ctttcaacga tggcttgagc aatcatgact gatgcatata agtcattggc acttgccaaa
    74821 ctttgtgcag ttgaggcaat actatcaatg aacgcttgtt gagaattagc agatgagcga
    74881 gcataaaaag catagccagt atagttttcg aattcagaca aatctgcttg atgggtatgt
    74941 gtcaatgttt gcccatctgt caattggttc gtgatttctt ctgcattctc tggattgttc
    75001 tgagcttctg ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt
    75061 tcttcggttg aaattgtatt ttctgagaca gtagctgact gcaaagtact ttctgtatcg
    75121 ttttcaattc ctgtttctga agaactcgat gtctgagttg cgacagtttc tgtcgattct
    75181 tcagctagca ctgttgtgtt ggcaactgct agatttccta aaagaataac gctaaataat
    75241 aaattctttg ctttcatctt ttttctcctt tgaattatga aacttttcct aaataacagt
    75301 gtaactaaaa aagtaaaagc aagtcaatgt ccatatttaa ataacatttg tttcttttta
    75361 ataaatttgc tttttctgca taaaaagacg taaatgaaga atattcttct ccattcacgc
    75421 cttaaattga tggtcgtttg aataactatt taacaagtaa ttctctctgg atattatccg
    75481 ttatggatta tacttcctgt gtcgtcggac ggataatcat gttgctgaca gacatccggt
    75541 ctggcgtttc tatcgcaaat gctacagcag aagctatatc ttcagatgtt aatccccatt
    75601 ctttttgcgc ttcatgaagt tcaagagcag cctttttgtc gctgatggtt tgatataatt
    75661 cagtttgaac agctcctgga gagatgatcg tagatttgat gttattttgc cgttgctctt
    75721 gacgtaatcc ttccatgatt gcacggacag caaattttgt tccgcaataa acggcggaat
    75781 caggataaac aacatgtccc gccacagaat cagttgaaat gatatgccca gatttttgtt
    75841 tttccatgat tggaagtacg gcggaaattc catttaacac acccatgatg ttgatatcaa
    75901 gcatattttg ccattcatca cggtgacctt caactaatgg agcggttggc atgattcctg
    75961 cattattgta caatacatcg attcgaccga acttttccat cgctaaatcg ataactgctt
    76021 ggacttgtgc aaaatctctt acatctgctg tttgaatata tagctcagct tctggtaaag
    76081 attcttttat tgcttttagg cgatcttctc ggcgtgcagc tataactagt ttagcccctt
    76141 tttctgctaa tagcttcgtt gttgcttcac cgattccgct tgaagcgccc atgatgacaa
    76201 tcactttttc tgataaatca ttcattctat tctcctcctg ttttgaaaaa tctcttgctt
    76261 atactcacta tctcttattt catcttactg ttcaaataat ccgttcgaca ggtttgacga
    76321 ttttggcagg gattcctgca acgatggtat tgtctgggac atctttcgta acagttgcat
    76381 tcgctgcaac aatggcattc tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc
    76441 cgatccaagc atttttcttg atgtggattg gctttactga caagcctctt cgttcttttg
    76501 gatcggttag atggttcacc gtgatgaggt tgacacgagg accaatcaat actgaatctt
    76561 ctaaacagat gccacctaag tcaacgaaca tcacttgacg attgataaag acatttttcc
    76621 ctatactaat atgtttgccg aaatctgtat aaaaaggcag cagaacagtc accgtatcat
    76681 ctactttgtc actaatgata ttttctagcc gtttgcggat atctgctggt tcatggtacc
    76741 cagtattcag cgctgctaaa ggctcttcgt tttccgcttg gatctgatgg atcacttcaa
    76801 agagcggatc cgttactcga atctctttat tgacgatttg tttttcgatg aggcttgtca
    76861 ttttgctccc tccttttatc cttactttat aacggacggc ttatgatgtc taatacttat
    76921 aaaacatact taactattcc ttttaactat acttagtggt atgctttttt aaaaacagga
    76981 gggaaaaaat ggatattcgt atactgacgt attttttagc agtagctaga gaaaaaaata
    77041 tcagccgagc agcaaaatct ctgcatatca cgcaacctac tctctcaaaa caactgaaag
    77101 atttagaaga agaacttggt gtgacacttt tcactcgagg ctcccgagag atacagttga
    77161 cggattctgg tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa
    77221 cggcgaacaa tttaacaaat gacgcaatca tctctggaga aatctacatt ggcggaggcg
    77281 aaactaaagc aatctctttc atttctgata gccttcattc gttgatcaag gcgcatcctg
    77341 atatccagtt tcatttatat agcgggaatg cagatgatat catagacaaa ctggatagcg
    77401 gattacttga ttttggactc gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc
    77461 gactgccagc ccaagatcgt tggggacttt tgatacatcg gaatcatcct ttagcaaagc
    77521 aaacagtcat ccatccgcag gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt
    77581 cagtggacag tcaactagct gaatggctag gtttcagcat agatcagtta tcgatcgtcg
    77641 gaacatataa cttattgtac aatgcttctt tgatggtcga taacgggact tttggtgcat
    77701 tagctctaga tgggatcatc aatacttatg ggacggaaaa agtattcatc ccactcgatc
    77761 cgccattaca agcttcactg aatctcattt ggaaaaaaga ccagcctctt tctcctgctg
    77821 ctcgtaaatt tttagatgtc ctaacggaga aatacagtta agtagagttt ttttccttcg
    77881 ctttcagctt tttaacaacg aataagttaa gcaaaagatc caatgattaa ataacataag
    77941 tttcttaatt tgttccttcc ttttatttct gataattcca gtaaattact ataattataa
    78001 aaaaagaatt gacaaaacat aactgtctat ataaaattga ttacgttaac aataattcgt
    78061 ttcatgtgac tagatcatac taggcatgaa aaatctctga ggaacatggt ttccctagga
    78121 ggtttttcgt gtctattttt gtttggccgc aatcaactgg tctagcaaac gtgaaaacca
    78181 aatcttcaaa aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt
    78241 ggtagatgac ggtggacagg aaaaagtagc tattggaaaa gggattggat ttgacagaaa
    78301 aagaaatgat ttgatttttg atcgtgatgt cgaacggata tttgtcatgg aaccagaagg
    78361 tcaaatcaag ttgcaaaatt tacttaatca gattgatgaa cgctttttat ttgcagcaga
    78421 aacgatcatt gatcatgcgg aaactgtttt gatggaaaag ttgaatgaac atgtattgat
    78481 cgctttgacc gaccacattg ctttttctgc ggagaacatt aataacggta tcgtcatccg
    78541 caacaaactt ttaagagaaa tcgaagtgct ttacagtgat gaattcagta ttgctcaatg
    78601 ggcagtagag tatctgacaa aagagcttgg gattccatat acttatgacg aagcaggata
    78661 tatcgcaatc catattcaca gcggacgaag cggtcggaac aataaccatc gcagtatccg
    78721 tgaagtgacg attgtatctg acatcatcca tttagtggaa tcagaattgg aaacagatat
    78781 ccattcagaa ccattttcac tgaattattc aagactggtg aatcatttac gactgcttct
    78841 acaacgaacc catgcgcaac aatatgctgt tttagacacg gaaatcgtag aaatggtcaa
    78901 acgaaaatac ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga
    78961 ataccagctc gcgatcacga aagaagaact gggttacttg gccatccata tcgagcgttt
    79021 aagaagcgca atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa attgaaagca
    79081 tatctacaac gaatgggacg ttcccttcaa cttccagttg cggtcttacc tgctgctgct
    79141 ttactagtag ggatcggaaa ctggtgggca tcttatagta acgacattgt cgctcatttt
    79201 ttacaagctg gcggtaacgc agttttaggt caattacctt tgctgtttgc cgttgggtta
    79261 gcactaggga tgtcagtgga caaatcaggt gctgctgcct tggcaggttt ggtcgcattt
    79321 gaagttccag taaatgtttt aaaaactgat tcagtagcta ctttattgaa tatcaaacca
    79381 gaatcagttg atccatcttt tgctcagatc agtaacgtat ttattggtat tattgccgga
    79441 ttgattgcgg cagctcttta taatcgtttc cacgaagcaa aattaccgat ggctttatct
    79501 ttctttagcg gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt
    79561 gcagtgctac tgctcgtatg gcctccggtt tactctgctt tagtttcttt tgctaaattc
    79621 attttgagtt taggctgggg tggtgcaggt ctttatggct tcttcaaccg tttactgatc
    79681 cctacaggat tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc aggaatcaat
    79741 gatattggta aatttttggc ttctgaagga acaaaaggcg taacaggaat gtatcaagct
    79801 ggatttttcc caatcatgat gttcgggctt ccagcaggag catatgctat ttacagaaat
    79861 gcacgtccag aaaacaaagc aaaaacagct tccttgatgt tagcagcagc ttttgcttca
    79921 ttctttactg gtgtaacaga accacttgaa ttctcattta tgttcgttgc ttggccatta
    79981 tatgtcttgc atgcaatttt cactgggatt tctttagcag tgagtgcatt cttccattgg
    80041 acagcaggat ttgcctttag tgccggattt gtcgattact tcctatcttt gaaaaatccg
    80101 attgcgaatc agccattgat gttgatcgtc caaggattag taacagcagt gatttactac
    80161 tttggcttta actttgctat caaaaaattc catctgatga ctcctggccg cgaggaagca
    80221 gatctttcag atgacgatac tgcaacgact aattcagaca ataaatatgc ggcacaagca
    80281 agcaaaatct acgctgcatt aggcggagcc gacaatgtaa cttcgatcga taactgtact
    80341 acacgccttc gtttgcaagt caaagataca ggcctgatcg atcaaaataa aatcaaagcc
    80401 actggcgtac ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca
    80461 gaagtacaat tcgttgcaga tgagatggca aaactacatg gtggtgctgc aacacgacct
    80521 gcacaaacaa acaccgtggt taaaacagaa acatttgccg gtggaacgat cgaacaagat
    80581 atttacgcta tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga tgatgtattc
    80641 gcggaaaaaa tgatgggtga cggctatgct gtattaccag aaaatggaga aatcttctct
    80701 ccaatcgctg gtacgatcac aaacattttc ccaacgaaac atgctgtcgg aatccaaaca
    80761 gatgctggta tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa
    80821 ccattcactt tatatgtaaa agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta
    80881 gttgatcttg ccgctattca atctgcaggc aaaaacacgg atatggtcgt tgtattcaca
    80941 aatggcgaca aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat
    81001 aaaatcggct ctgtttccaa caaagcctga gccatcgtct tccccagact tttttatcga
    81061 taaacaaaca ggagctgaga caaaaatctt gtctcagctc ctttttccga ataaaaacgg
    81121 cgttctcctg ccaactccgc ggtaataagt ccagccaaac gtaacatatg aagagctatt
    81181 ttttatttga ctcttcttat tacttgatgc agaacagctt ttttacaacc tctgataaac
    81241 ggtgttcaaa aaccagttta tcggtatgaa gtcaaattta tgcaaaaatg tggaaagcca
    81301 ttttcacaaa atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac
    81361 attattcctg atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca
    81421 atactttttc taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga
    81481 tcgctaaaaa catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa
    81541 acgctgcatc acttgcttca ataatttcta actttttacc tgtcgcacgc tctaatgcct
    81601 tggctaattc ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac
    81661 tttctactag tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt
    81721 ttccatttcc tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta
    81781 attcattctc tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa
    81841 tcattttttc agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg
    81901 caaaacttgt ataagcgata taagataccc cagctgcttg tgctgccttc actacattct
    81961 tatgttcggc ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt
    82021 tcaacgcttc tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag
    82081 cttctttcag tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg
    82141 gaaccatctt ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta
    82201 ctaaataatg catatttgcc actccctatt ctttttattt gttaagattt acagctgtac
    82261 atttataaat tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg
    82321 aaaggatgac aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc
    82381 tatattgaaa tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc
    82441 gaaacaaatg cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc
    82501 tttacaaaaa ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta
    82561 ctagatgtct atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca
    82621 aatccaaatt gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg
    82681 ctgcagaaaa aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa
    82741 atagccgttt tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta
    82801 taattctctc tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat
    82861 tctccggggg acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc
    82921 gctgaagtca tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct
    82981 gaaaaatgca aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag
    83041 cgctactttc acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc
    83101 ccatatattt cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg
    83161 cgtaatcttc tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta
    83221 attcttcagg cgattctgct aaagcgatta caacaagata cgttcctttt gggaatggga
    83281 tgattcgggt agtgtttgga acatcctgac tcgtttgaac accgacatag tacatcattt
    83341 tcccgttata gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg
    83401 catctattgt tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac
    83461 cgacaaaatc cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg
    83521 gtttttcttc gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat
    83581 cttacataaa attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat
    83641 tttttaacta tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat
    83701 cctctcccca ttttgtaaaa gagatctcgg gagcttcgac taactcataa tctgaagaag
    83761 gaagccattc tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt
    83821 tattaggaaa aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg
    83881 gattttcttt tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc
    83941 gggctccatc aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact
    84001 tatgcatctc ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat
    84061 tgactccttc aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt
    84121 tcttttcgat acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc
    84181 gtggaaaaga tttttgtatg ccgtatctgc tgatttctga aggcagatat cctgaccaat
    84241 cacgaaaagc tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg
    84301 tcactcttga tccatctagg agatcgcgat tagccaaagc caatctacgg tttttgatat
    84361 agtccattag cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga
    84421 ttcctgtatt ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt
    84481 aatcgatcaa ttggttcaga tctttaagca tatttatctt cctccttgct tgccttttta
    84541 tcatattatc aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag
    84601 aaaatgttgt atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta
    84661 ttatctgcct atctatgttt tacctttaga aaaataaatt ttggggaaga cacattctaa
    84721 gtaaacaaag cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt
    84781 acaaagcaaa ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat
    84841 acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt
    84901 ttgtcagata aataaa
//
LOCUS       DAFCNJ010000006        83001 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000006 DAFCNJ010000000
VERSION     DAFCNJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.006"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..563)
                     /locus_tag="LNE35_000545"
     CDS             complement(<1..563)
                     /locus_tag="LNE35_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBK6909730.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="LNE35_000546"
     CDS             complement(750..1400)
                     /locus_tag="LNE35_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909731.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LNE35_000547"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LNE35_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBK6909732.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="LNE35_000548"
     CDS             complement(2912..3910)
                     /locus_tag="LNE35_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBK6909733.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LNE35_000549"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LNE35_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBK6909734.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="LNE35_000550"
     CDS             complement(4926..5657)
                     /locus_tag="LNE35_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBK6909735.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="LNE35_000551"
     CDS             complement(5661..6926)
                     /locus_tag="LNE35_000551"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBK6909736.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="LNE35_000552"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="LNE35_000552"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBK6909737.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="LNE35_000553"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="LNE35_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBK6909738.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="LNE35_000554"
     CDS             12268..13770
                     /locus_tag="LNE35_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBK6909739.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="LNE35_000555"
     CDS             13772..14509
                     /locus_tag="LNE35_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6909740.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="LNE35_000556"
     CDS             complement(14554..14727)
                     /locus_tag="LNE35_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909741.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..15368
                     /locus_tag="LNE35_000557"
     CDS             14703..15368
                     /locus_tag="LNE35_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909742.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="LNE35_000558"
     CDS             complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="LNE35_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBK6909743.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="LNE35_000559"
     CDS             complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="LNE35_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBK6909744.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="LNE35_000560"
     CDS             complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="LNE35_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBK6909745.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="LNE35_000561"
     CDS             complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="LNE35_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBK6909746.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20581..21747)
                     /locus_tag="LNE35_000562"
     CDS             complement(20581..21747)
                     /locus_tag="LNE35_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBK6909747.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21763..21990)
                     /locus_tag="LNE35_000563"
     CDS             complement(21763..21990)
                     /locus_tag="LNE35_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBK6909748.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22474..23064
                     /locus_tag="LNE35_000564"
     CDS             22474..23064
                     /locus_tag="LNE35_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBK6909749.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23254..23967
                     /locus_tag="LNE35_000565"
     CDS             23254..23967
                     /locus_tag="LNE35_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909750.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24082..25092)
                     /locus_tag="LNE35_000566"
     CDS             complement(24082..25092)
                     /locus_tag="LNE35_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBK6909751.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25129..25611)
                     /locus_tag="LNE35_000567"
     CDS             complement(25129..25611)
                     /locus_tag="LNE35_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBK6909752.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25761..26129
                     /locus_tag="LNE35_000568"
     CDS             25761..26129
                     /locus_tag="LNE35_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBK6909753.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26594..27046
                     /locus_tag="LNE35_000569"
     CDS             26594..27046
                     /locus_tag="LNE35_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909754.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27170..28021)
                     /locus_tag="LNE35_000570"
     CDS             complement(27170..28021)
                     /locus_tag="LNE35_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBK6909755.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28034..28819)
                     /locus_tag="LNE35_000571"
     CDS             complement(28034..28819)
                     /locus_tag="LNE35_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6909756.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="LNE35_000572"
     CDS             complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="LNE35_000572"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBK6909757.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30116..31039)
                     /gene="truB"
                     /locus_tag="LNE35_000573"
     CDS             complement(30116..31039)
                     /gene="truB"
                     /locus_tag="LNE35_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBK6909758.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31407..33769)
                     /locus_tag="LNE35_000574"
                     /pseudo
     CDS             complement(31407..33769)
                     /locus_tag="LNE35_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34217..34390
                     /locus_tag="LNE35_000575"
     CDS             34217..34390
                     /locus_tag="LNE35_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6909759.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="LNE35_000576"
     CDS             complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="LNE35_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBK6909760.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34921..37227)
                     /gene="infB"
                     /locus_tag="LNE35_000577"
     CDS             complement(34921..37227)
                     /gene="infB"
                     /locus_tag="LNE35_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBK6909761.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37240..37551)
                     /locus_tag="LNE35_000578"
     CDS             complement(37240..37551)
                     /locus_tag="LNE35_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBK6909762.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37548..37841)
                     /locus_tag="LNE35_000579"
     CDS             complement(37548..37841)
                     /locus_tag="LNE35_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBK6909763.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="LNE35_000580"
     CDS             complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="LNE35_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBK6909764.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="LNE35_000581"
     CDS             complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="LNE35_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBK6909765.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39679..44037)
                     /locus_tag="LNE35_000582"
     CDS             complement(39679..44037)
                     /locus_tag="LNE35_000582"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBK6909766.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44249..45958)
                     /locus_tag="LNE35_000583"
     CDS             complement(44249..45958)
                     /locus_tag="LNE35_000583"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBK6909767.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="LNE35_000584"
     CDS             complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="LNE35_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBK6909768.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47455..48255)
                     /locus_tag="LNE35_000585"
     CDS             complement(47455..48255)
                     /locus_tag="LNE35_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBK6909769.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48252..49064)
                     /locus_tag="LNE35_000586"
     CDS             complement(48252..49064)
                     /locus_tag="LNE35_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBK6909770.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49259..49816)
                     /gene="frr"
                     /locus_tag="LNE35_000587"
     CDS             complement(49259..49816)
                     /gene="frr"
                     /locus_tag="LNE35_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBK6909771.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49819..50541)
                     /locus_tag="LNE35_000588"
     CDS             complement(49819..50541)
                     /locus_tag="LNE35_000588"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBK6909772.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50677..51558)
                     /locus_tag="LNE35_000589"
     CDS             complement(50677..51558)
                     /locus_tag="LNE35_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBK6909773.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="LNE35_000590"
     CDS             complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="LNE35_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBK6909774.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52798..53277
                     /locus_tag="LNE35_000591"
     CDS             52798..53277
                     /locus_tag="LNE35_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBK6909775.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53494..54585
                     /gene="phnW"
                     /locus_tag="LNE35_000592"
     CDS             53494..54585
                     /gene="phnW"
                     /locus_tag="LNE35_000592"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBK6909776.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54578..54706
                     /locus_tag="LNE35_000593"
     CDS             54578..54706
                     /locus_tag="LNE35_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK6909777.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54710..55255
                     /locus_tag="LNE35_000594"
     CDS             54710..55255
                     /locus_tag="LNE35_000594"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK6909778.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55709..57400)
                     /locus_tag="LNE35_000595"
     CDS             complement(55709..57400)
                     /locus_tag="LNE35_000595"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBK6909779.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="LNE35_000596"
     CDS             complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="LNE35_000596"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBK6909780.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58882..59901)
                     /gene="argF"
                     /locus_tag="LNE35_000597"
     CDS             complement(58882..59901)
                     /gene="argF"
                     /locus_tag="LNE35_000597"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBK6909781.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="LNE35_000598"
     CDS             complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="LNE35_000598"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBK6909782.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            61682..62383
                     /locus_tag="LNE35_000599"
     CDS             61682..62383
                     /locus_tag="LNE35_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6909783.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(62992..64008)
                     /gene="tsaD"
                     /locus_tag="LNE35_000600"
     CDS             complement(62992..64008)
                     /gene="tsaD"
                     /locus_tag="LNE35_000600"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBK6909784.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(64005..64469)
                     /gene="rimI"
                     /locus_tag="LNE35_000601"
     CDS             complement(64005..64469)
                     /gene="rimI"
                     /locus_tag="LNE35_000601"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK6909785.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(64476..65018)
                     /gene="rimI"
                     /locus_tag="LNE35_000602"
     CDS             complement(64476..65018)
                     /gene="rimI"
                     /locus_tag="LNE35_000602"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK6909786.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(65002..65724)
                     /gene="tsaB"
                     /locus_tag="LNE35_000603"
     CDS             complement(65002..65724)
                     /gene="tsaB"
                     /locus_tag="LNE35_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBK6909787.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(65915..66895)
                     /locus_tag="LNE35_000604"
     CDS             complement(65915..66895)
                     /locus_tag="LNE35_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6909788.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(66919..68403)
                     /gene="galT"
                     /locus_tag="LNE35_000605"
     CDS             complement(66919..68403)
                     /gene="galT"
                     /locus_tag="LNE35_000605"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBK6909789.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(68415..69404)
                     /gene="galE"
                     /locus_tag="LNE35_000606"
     CDS             complement(68415..69404)
                     /gene="galE"
                     /locus_tag="LNE35_000606"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBK6909790.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            69652..69819
                     /locus_tag="LNE35_000607"
     CDS             69652..69819
                     /locus_tag="LNE35_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBK6909791.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(69881..71692)
                     /locus_tag="LNE35_000608"
     CDS             complement(69881..71692)
                     /locus_tag="LNE35_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBK6909792.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(71689..72054)
                     /locus_tag="LNE35_000609"
     CDS             complement(71689..72054)
                     /locus_tag="LNE35_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBK6909793.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(72217..72612)
                     /locus_tag="LNE35_000610"
     CDS             complement(72217..72612)
                     /locus_tag="LNE35_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK6909794.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(72630..73592)
                     /locus_tag="LNE35_000611"
     CDS             complement(72630..73592)
                     /locus_tag="LNE35_000611"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBK6909795.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(73592..73804)
                     /locus_tag="LNE35_000612"
     CDS             complement(73592..73804)
                     /locus_tag="LNE35_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBK6909796.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(73825..74523)
                     /locus_tag="LNE35_000613"
     CDS             complement(73825..74523)
                     /locus_tag="LNE35_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBK6909797.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(74543..75085)
                     /locus_tag="LNE35_000614"
     CDS             complement(74543..75085)
                     /locus_tag="LNE35_000614"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBK6909798.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(75217..76221)
                     /locus_tag="LNE35_000615"
     CDS             complement(75217..76221)
                     /locus_tag="LNE35_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK6909799.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(76218..77207)
                     /locus_tag="LNE35_000616"
     CDS             complement(76218..77207)
                     /locus_tag="LNE35_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK6909800.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(77204..78010)
                     /locus_tag="LNE35_000617"
     CDS             complement(77204..78010)
                     /locus_tag="LNE35_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909801.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            78176..79132
                     /locus_tag="LNE35_000618"
     CDS             78176..79132
                     /locus_tag="LNE35_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6909802.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(79209..79727)
                     /locus_tag="LNE35_000619"
     CDS             complement(79209..79727)
                     /locus_tag="LNE35_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK6909803.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(79815..79964)
                     /gene="rpmG"
                     /locus_tag="LNE35_000620"
     CDS             complement(79815..79964)
                     /gene="rpmG"
                     /locus_tag="LNE35_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK6909804.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            80192..80638
                     /locus_tag="LNE35_000621"
     CDS             80192..80638
                     /locus_tag="LNE35_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK6909805.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(80833..82728)
                     /gene="asnB"
                     /locus_tag="LNE35_000622"
     CDS             complement(80833..82728)
                     /gene="asnB"
                     /locus_tag="LNE35_000622"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBK6909806.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        23581 a        17673 c        14335 g        27412 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacatttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaccaggcga acatatgatt gttcgtctgg tttttaatta
    15421 ttaatgactt agcgttttta ctagaaaaac tacttccgct ttttcccgcc aaaagcatct
    15481 ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc gccagcttca
    15541 gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt gatcagtttt
    15601 actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc ttttccacgt
    15661 aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac atctccatgg
    15721 acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa aggcaattca
    15781 tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg gaagaccaca
    15841 tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc taaacgcatt
    15901 tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc tttcttctca
    15961 tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt cccatgacaa
    16021 acatcacaag tttgacggct catcactcgt ccaagtggtg tttggcgttc tacattaatc
    16081 gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt tgcaccgttc
    16141 ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc tacaccaaaa
    16201 atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg acgaggcgca
    16261 tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc ttcaaaacca
    16321 ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc tccaccagca
    16381 ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat atgcagcgcg tttttgcggg
    16441 tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc atcaggttct
    16501 ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt tatttcatct
    16561 tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt tgccataggc
    16621 tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat attttgtttg
    16681 aaacaaaaac cgatacttca aactattttt caaaagaaaa agccaaagta gcaactttgg
    16741 ccttctctcg aaattcagtg aactgattat ttatcgtcgc catcgacttc ttcaaaatca
    16801 gcatcaacga catcatctgc tccgccttga gctgcttctg ggttttcttg agcttgttgt
    16861 tgtgccgctt gttcgtatag tttaacagtc aagttttgaa cgatttcgtt caatgcatcg
    16921 cgtttagttt tcatttcttc gatgttgttt gcttcaacag cggctttcaa ttcatcgcga
    16981 gctgtttctg cttttttcac ttcttcttcg tccactttgc cttctaattc tttcaatgtt
    17041 ttgtccacag agaatagcaa tgcatctaca tcattacgta ggtcaacttc ctctttacga
    17101 gctttatctg cttctgcatt tgcttctgca tcttttacca tacgttcgat ttcttcgtct
    17161 gttagacctg aagaagattt gatcgtgatt ttttgttctt tttgtgtacc tagatctttc
    17221 gcagatacat ttacaatccc gtttttatca atatcgaatg ttacttcgat ttgtggaata
    17281 ccacgaggag ctgctggaat atctgtcaat tggaatcttc ctagagtttt gttgtcagca
    17341 gccattgggc gttcaccttg taatacatgg atgtctactg ctggttgatt atctgccgca
    17401 gttgagaata cttgtgattt acttgttgga atcgttgtat tgcggtcaat caatttagtg
    17461 aacactccac ccattgtttc gatacctaat gacaatggtg taacgtcaag taaaacaaca
    17521 tctttcacat caccagtgat cacgccacct tggatagcag cacccatagc aactacttca
    17581 tcagggttaa ctgatttgtt tggttctttt cctgtttctt tacgtactgc ttctacaact
    17641 gcagggatac gagtagatcc accgactaaa ataacttcgt cgatttctga ttgtgataaa
    17701 cctgcatctt ttagcgcttg acgtactggt acttttgtac gttctactaa gtcagctgtt
    17761 aattcatcaa attttgcacg tgtcaatgtc atttccaaat gcaatggtcc agattctcct
    17821 gctgtgataa atggcaagct gatttgtgtg cttgatacgc cagataaatc ttttttcgct
    17881 ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt cttgagacaa gtcaatgctg
    17941 ttttcttttt tgaattctgc taccatgtag tcgatgattt tgttatcaaa gtcgtcccca
    18001 cctagatggt tgtcgccagc tgttgacaat acatcaaaga caccgtctcc tagttcaagg
    18061 atagatacgt caaaagtacc gccaccaagg tcgaatacta agattttttc gtcgcgatct
    18121 gttttatcta aaccataggc aagagcagct gcagttggtt cattgacgat acgttctact
    18181 tctagaccag cgatcttacc agcatctttt gttgcttgac gttgtgcatc attaaagtaa
    18241 gctggcacag tgataactgc tttttcgact ttttcaccta aatagtcttc tgcgaatcct
    18301 ttgatatatt gaaggatcat tgctgagatt tcttgtggtg tatatgattt accttctaca
    18361 tctactttgt aacctgcttc accgatatgg cgtttgattg atgaaatcgt atttgggttt
    18421 gtcacagctt gacgttttgc aacttcccct acttggattt ctccattttt gaatgataca
    18481 acagaaggtg ttgtacggtt tccttctggg ttagcgatga tttttgcttc gtttccttcc
    18541 aatactgcta ctgcagagtt agtcgtacct aagtcaatac cgataatttt actcataatt
    18601 aacgtctcct tctttaattg aaagtgtaag ttttatatcc gcacaattat tgtgcaacaa
    18661 tgaccatggt tggtcttaac acgcgatcat gtaatttgta gccttcttgt agtacttcta
    18721 caatcgtatc ggctggtgta tcttcagtcg ctggaacagt ttgtactgct tgatgaagat
    18781 taggatcaaa ggcttctcct ttagcaggga ttttttcaat tccttcttct tccagtgcgt
    18841 tacgcaaact ttccaagacc atttccacgc cttttttcaa acctgcccct tgatcatctg
    18901 acacttctgt ggctaatgca cgttccaggt tgtcaattga tggaagtaat tttttggcta
    18961 aatcttgtga acggtatttt tgtaattgct cacgctcatt ttttcctcga tttgccatat
    19021 ttgcgatttc agcacgagcc cgcaagtatt tatcttccat ttcttctaac tcagctttta
    19081 atttcgctgt ttcgaattct tccgcttcag cttctgaaat acccgaatct tctgcagctt
    19141 cgacatcgat ttccggttct ggctgagcat ctgtcatttc tttatctagt tcttcttgat
    19201 tttctttcac gatacagtct tcctttcatt attcagtcag ctgtttgatt gctcttacta
    19261 agaaagagag aacgatcagc tgcaaatagc ttcgttattc acattacgag tttgacaagt
    19321 caagcgaacg gtagtaatca gcaagtttag ctgctagttc tcttcgataa acatcgacta
    19381 gaccgaacat ccgagaataa ggcatactag taggccctaa caaggctatt gtacctcttc
    19441 catgaccatc tatctcgtaa cttgcttgaa tcatactcat attctgcaat aaatcattgc
    19501 ctaattcagc accaatcttg atatgaatat tgctatccat cggagtgatc atttgtgtaa
    19561 gtccgtctga gtctttcata aaagaaaaca tcgatttgaa ctgatcaaga tcttgacggg
    19621 gctcataatc caagagattc atttgtccac tgacataaac cttatcttca aaaacatgac
    19681 caagaacggt gtcaaataac tgagacatcc cttctgtcgt ttggaaatat ttatgaagaa
    19741 tcatcggaat ctccgtacga agtttatggt acaccgtcat cagaggatct ccgactagac
    19801 ggtcgtttac aatctgtacc attttttcta gttcctgact tcccatatat tcgggaagag
    19861 tgaacacttg gctttctaca ttccccatat ctgttacgac gatagcaata atctgtcgat
    19921 tattcaaggg tacgatacga aagccagtca gtcggcggtc ttttatttca ggtcccaatg
    19981 aaaaagtcgt atagcttgtc agctcagata atatctcagc agattgctta ataatctcat
    20041 tgatttcatg aaattctctg cctagagact gacgaatcag cagcaaatca tcttctgcta
    20101 cgcgagcagg ttttaacaaa tgatccacgt aataacgata gcctaaagca gatggaatcc
    20161 gcccagaaga agagtgagtc ttcaacagca atccttcatc ttctaatgct ttcatatcat
    20221 tgcgaatcgt tgctgagctg gccttgactc cctcagacat caatgttttg gaaccaacgg
    20281 gcacgccact actcgtatag tgttggatga tcagacgtaa aatgtcgttt tgtcgctttg
    20341 ttaacatctt cctcacctct cattagcact catatattct aagtgctaat ccacttataa
    20401 atatagcaag aagcccttct cctgtcaaga gaaagactcc attttttagc actctaaaca
    20461 caagagtgct aattctaaat aattgatgta aatccagtta tatcaacgtt tcaatagtcc
    20521 tttattgtat ttttgaactt tttttattta aattcagata aaaaacaaat attctttaaa
    20581 ctatttttca aacaaaaagc gctcaaacac attgttcccc aaaaaggtcc ctcttggtgt
    20641 caaataaaca cggtcttctt cattaacaag taacccttct tgctctaatt cccttagcac
    20701 atcaccgtac accgattcaa ttgttttttg atagcgatca tagaaatgtt ttttagtaat
    20761 ccctgttttt tttcttagtc ctaagaacat ttcttcttct atttgatttt ttcgtgtcaa
    20821 tttttcttct tccaataccg ggcgttcatt tgctcgtaat gggcgtaaat agtgttggat
    20881 tggtcctttg ttgcggtaac gaatatctcc aagataaccg ctagcaccag ctccaaaccc
    20941 aaaatagtgg tcattgttcc agtacatgag gttatgttga gattcatgtc cggagagccc
    21001 aaagttactt atttcatatt ggtgacgtcc atgcttttcc attgcttcaa tcgtctcttc
    21061 aaacattctg gtttccgttt cctgatcagg cagttcgagt cttccttgtc gtacccagtt
    21121 catgaacatt gttttgtttt cgaggatcaa tgagtacaaa gaatagtgag gcagatccaa
    21181 agccaaagct ttttccaaag tatccagata tccttctagt gtttgtcccg gtaatgcata
    21241 aatcaaatcg atgcttacat tggtaaaatt ttctgcttct aaaaattgca tagtttgata
    21301 cacatcttct gcggtgtgtt tacgtccaat ttttttcaat aagcgattat caaatgtctg
    21361 cactcccatg gataaacgat tgacgccata gtttttcatt acttgaagtt tttcacgggt
    21421 caaatcacct ggattcgctt ctacagtaaa ctcattacca ttttgaaaag gcaggatctc
    21481 tctagcacct gataacagtc gatcaagttg tttagcagat aaagaagttg gtgtcccgcc
    21541 ccctacgtaa agtgtttcta aattcgctgt cggatgactt tctagcatta ttttcatttc
    21601 attcagcagc atttccacat actcatctac tggctgccct tctaaaaaaa ctttattaaa
    21661 atcacagtaa tagcagatat gctcgcaaaa tgggatatga atgtacgcag aaaccttgtt
    21721 ttctgcggga tttattgttt ctatcattga aattctttct ccttacgtcg aaaagtcgtg
    21781 cctgttttcg accttcttaa aatcgcgttc aagcaccaac tcattgaaat aagccgcata
    21841 tttagaatca aggaaaccta gctctaaaaa tgcttgctta ttgtcatcgg agctacacgg
    21901 tgcttttaca gcctctcgat aaacggtaat cgcaaagctg accttcggca ttaagtcaaa
    21961 atctgcaaaa atatgaaaag ctattttcac agattcctcc ttaagtgctc agaccacaac
    22021 gctttgctta caacctctca tatacgcata ctaaacgtat ttggcgaaca aatccactaa
    22081 aaaatccaag aatcgacttc ttggatgatt ttttctttca ttaaactggt tacctagtta
    22141 tatatctcta gggttgtttt catgtctgag tgcccgatat gatttatttt tcgtttctat
    22201 aattactttt tgccttccag tttcttttaa tattgtactc aagcaagtgg gaattggttc
    22261 ttttgtcatc catgtatgca aaatgttata atgctggtaa acaaaaatga ttacctgtca
    22321 tttttattta gttcgtattt aattaagttg actatctgta tactctgtct aaaacttaag
    22381 aattgttgtt ttcgctgtcg ctacccaatt ttaatgaatg tctgtaggtt aaaactgttg
    22441 tccttgttat agatagtaaa ggagagcttg aaaatgaaaa ctgcgattac gtttctatta
    22501 gaggaatatg ccgattggga aggtgcttat attactagca cactaaacca aaatgagaaa
    22561 tggcaaaaca aaactgcttc tatccaatca gaggtcactt ccattggagg attaaaaaca
    22621 aaaatcgatt acttaatcga acaaatacct gaaaaagttg acctttttat tatgatcggt
    22681 ggcaattcat gggacataaa aaatcatcat ttatatcaaa taatcgataa attgttaaaa
    22741 acagacgtac cagtagctgc tatttgtggg gcagttgatt atttagctca gagcggatta
    22801 ttgcaaggtt acaaacacac cggaaatgcg caatttcctt ggttagaatc ggaaaattat
    22861 cagaataaag aagattttat agaacaacaa gctgtgaaag atcgtaattt gattactgcg
    22921 aatggaacag ctcctataga attcacagac ttgattcttc aagcagtcca ttttgaaaca
    22981 gaagaaataa gagaaaaaac gattcgtcta tacaaacttg gattctatga ttactataaa
    23041 aagtacggag atcctttctc ctaatcattc tcttcaatta aatactcctt atcaatagtg
    23101 tgacaagtaa ttttactgat gattatttgt cacacttttt ctgacattta tcaaagaaga
    23161 tacactctat ttatattcat agacaaggtt ttacggattc aaaaattcag gattatgcta
    23221 tactggtatt aaaaaaataa tgaggtattt tatatgaaaa agttaggttt tattttattg
    23281 ttggtctctt tattccttgt aggatgtacg tctcctagta aagaaaatag tgatccatct
    23341 gcgaccacag acaaaagttc aatcagacaa accagttcag ctgaaaaatc aagtactcag
    23401 aaatcagctt ctactgaaac gaatacatct tcaacaagca gtaaagtctc agagcccaat
    23461 acttcttcta catctagtag tacagttgag acaaatattt cttcttcaac aatagacaca
    23521 acctcctcac agtcttctca gtcaatagat ccgttagcag gatattctga tctacaaatt
    23581 gagtacgcac gtgtttggct agctattaga ggtaatactt ttagagatac atcgggtgat
    23641 ttcgaattac atgttattca ttatcctgca ggaacaccta tcgcccctta tgatgcaggg
    23701 agtgcagtct atccgaaaga tgtggttatg ctcactggga aatacagtta ccagggagtt
    23761 ttagtgtatt caagtaacca tgacgggaca atcactagtt atccagttcc ttcacattgg
    23821 caaatgcctg cagatcaaac atcttatcca gcgttcatta gagaaacaac tcaagaaatc
    23881 attgataatg catctgtagt agctattcct acaggaaatc ccaatgacgt gaaacagtta
    23941 atcgatgtga cagttataga taattagact ttctaatatg ttatacaaaa agaagctgac
    24001 agcattttgt cagcttcttt cactttcttt tctatttaga tttattggtc tttgatagac
    24061 tccaaggcag gaaaaaattt tttaaataaa cgtttgcaaa acagtcgctt ttctatcttc
    24121 taccgctact tctgcatagg caccattaat caatgtcagt tgtggcggta catgaccaca
    24181 atcaatatca tacaagatcg gtagttttaa ttcctttgac agatcttcgt atacatcctt
    24241 cattttataa tctaacaaat cctctgccgc acttctaccg aacatgatcc ccgagctgcc
    24301 atcaaaccag cctgccagtt tcatctgaac aagtgatctt cttaaatcaa ctgacgatag
    24361 ctcacagttt tctagaaacc acaagatcgg ctttccatca ttatactttc ttttaaaaga
    24421 ttggacatct ccataaggag taccgattaa atgcctgatc gtatcaatac aaccacctaa
    24481 taatcgtccc ttcatcctta ctgcatctcc gctgactgat tgccaatttg ttggttcagt
    24541 gagatggaaa acagaagatg atgaatgctc atggggccac actttttgat acttttcaga
    24601 agattgctgt agaatcgatt ctccttctct tgttttcagt acactttccc acattgctgt
    24661 cgtttcatcc aagtactctc ctcttaaatc aataaggtta gttccatgtg cagtggctat
    24721 ccctgttttt aatgtgacag caagtagcaa cgtactaata tctgaataac ctactatcca
    24781 ttttggttgt agagcatcaa aatcgacaaa atcgagaatc tcaatcaata attctcctcc
    24841 ccaagtcgga aagatcattt ggatattttc atcttgcatc atttgattga attcatctgc
    24901 tcgttttctt gctggacctg atttttcctt atcttgcgtc caaagcgtct ttccagtaac
    24961 tacttggaaa gatttttctt ccattctctt taaagctgat tcgagtaacg aatggtgttc
    25021 ctctgatacg cctgaggatg gtgcagtaac cccaatcgtt gcatttcttt ctaataatgg
    25081 ataacttatc atgttgcttc acctccaagt atgcactatt ttacttggtt aattgttttt
    25141 ttcgtttgcc ggcatcaaaa atatcggcat ctcaagtagc tggatgattt tgtttaagtc
    25201 aaccgtttgg atgaccatcg tttgatctgg tctacctggt gcaaaaacaa gtttttcatt
    25261 tgataagagc tcttcatcaa taataatcgg aatctgtttt tcataaccaa acggagaaac
    25321 agcgccctgc ttttgtcccg tttgttcttc catgcgttct gatgaaacaa tagaaagcct
    25381 tttaccgacg atcttcccta atgctttgaa atccgtttgt ttcgtagtaa aagtcagata
    25441 tacgtagtta ttatcttttt tatcttttaa gaataagctt ttggtctctg tacctgtaaa
    25501 tccttgttct ttctttatta ccaaagcatc ttcatttgta taaatggctc gatgatgata
    25561 caactcaaaa tatatatttt cgtgtaagaa tttctttgcc aagtcaatca tttttatgtc
    25621 tcttttctat tttattattt gagacatctt atcatataaa tatagcggtt acaacattac
    25681 ttgtgttttg ttcgtttttt atacataact attgtttttc tggtaaaata aatcaaacca
    25741 cttaatggag gcgttttaga atggaaaaat ttcagccgat catcaatcaa atcaaagaaa
    25801 aagaacatca acaaaagatc aaagaccttt ttgctaagat aacagaaacg tatccccagc
    25861 tagacacaac tgtaaaatgg aaccaaccta tgtttactga ccatggtacc tttattattg
    25921 catttagttt tgctaaaaat catttttcta tcgcaccgga aaaagcagcg attcgtgccc
    25981 ttgaaaaaaa tatccaagaa gcaggctacg tatatacgga taacgtcatc aaagttcctt
    26041 ggaaaagtgt aatcaactgg gaattgattg aacaattgat ttcatttaat attgaagata
    26101 aaaaaggtca tgataaattt tggcgataaa gctttatacg aataaacctt ttactggcga
    26161 ggaaactatt catcacctta tacaggtttt gttcaatcct atccttttta tcatccctgg
    26221 ttaaacactt aattgacaaa gtatctgcta tttttataag gagtctttct tgtgctgaaa
    26281 aaaatcactg attgaatggc tttgaatttt ctacatagta aaattatttt ttcagcaatt
    26341 ttcctgcaaa acttttgaat aaggaaaaaa tacgagaaaa tatcagtgat ctttcataat
    26401 actgcccatt attttcaggt atatttaaat ataatatcac tttaggagag aacgaatttc
    26461 tttagagaaa taaaagttag aattttctga cttgattttt acaatgacct taattaaaat
    26521 cgttctttct acatttcgtg attaagcaaa tatcaactaa gtcagtagaa ccattcacat
    26581 aggagggtga acattgaaag atttttactc agtaaatgaa ttagccgaac aattaggtgt
    26641 tactacacgt tctatcagaa attatcttca tgaaggaaaa ctaaaaggaa caaaggttgg
    26701 cgggcaatgg aaattttctg aacgtaatct ttttgagttc ctatacggag atcaagcaga
    26761 ggaagcagca aaagaaatgc agcgattcat gctaaatgca ccaatcacga tgcgtttcaa
    26821 ccttcaatat cgtgatttta cagcaatcaa tcaattccga gaacaattag tccagtacca
    26881 taacgatgtt tacgcaaata aaaaagaccg tcttttacaa tacgatcttt ataaagacaa
    26941 tcatgcggag atactaatcg gtggtaactt caattatgtc gtaaacttta gtcaatggat
    27001 caatgaaaaa ttattgatcc agacagatat ttcccttgta tcttaaacaa ttaaaaagcg
    27061 ctagaaattc cagagatgtt ggaaattcta gcgctatttt tttataagta cttactgttt
    27121 ggttcaaaca aatatttttt taataaatga aattttacca tctttatcct caaataaaga
    27181 ccgtgaccat gcttcctact acgatcagca ataatccgat aatcgttaat tttttttctt
    27241 ttggtgtctt attttcatgc aggatgtaca taccgcctaa tgtagagata actacgttta
    27301 gctgtgtaat gatatatgca gtagcaaccc cattttcatt tgctgaaaaa atataagcca
    27361 atgctgacac actaaagatg gctccaacga tcgttgcttt ccaactcttg tcttcagaaa
    27421 atgcggttgg tttacggaaa atcacataaa tcactgcccc taaaaagaca ccgatcattt
    27481 gcggaaagaa aatggaaagt ccgcttgcat caactagttt aggcaatgca ctgtataccc
    27541 aataaccaat acttgttgat gctaatagac taattccttt tgtgataccg cctttttttc
    27601 caggatcatc actgattgcc gttaaagcag aaccaaatac aagaagaatg attgctgctg
    27661 ctccaatgat tttccccata cttccagacc attctccaaa agcgaagaca ccaatcaacg
    27721 aggttccgac taactgtaat gcagtcgaaa ttggcatcgt tttagaaaca ccaatcaagt
    27781 tgaaagatgt atactgtcct gtttgtccaa taacccaaaa tgcaccagaa agtaaactaa
    27841 tcaaaaatgt agtcactgaa ataccagctg gagaagcgaa taattgaacg agtaagccga
    27901 caagcaccgc tccaatacca gttcctaata tctcatttgt tggtctgcca ccaatctttt
    27961 tcaaaatcag tggctggatc ccccagccaa ctgccgggat caatgcaatc aataaaccca
    28021 tttttattca cccttatcct tttccacctt ggaaagaagg atataaagtc atcccaccat
    28081 ctgcaaatag tgtaatacct gtaacataac tggattcgtc tgaagccaac catgcagctg
    28141 tcgccgctat ttcttttggt tcacctacgc gtcctagtgg gatcatactt tccaagagtt
    28201 ctttttgttc aggatcatca aattttgccg cattaatagg tgtacggata gcaccagggc
    28261 ctatactatt gattctgatt cccttcggtg catactccat tgctaccgtt tcggtaaata
    28321 attttacccc gccttttgaa gcagcgtaat gagcaaacgt tggccatggg atctgttcat
    28381 gaacagaaga catattgatg atatttcctt ttttattatt ctctgtaaag taatttaaag
    28441 ccgctttcgt tccgataaaa acgccagtca aattgacttt taacactctt tcccaatctt
    28501 caaggctaag ttcatgggtt ggatgctgat tttccatccc agcattatta acccaaacgt
    28561 ccagtgtccc aaaattttct aaagccgtat caagtaaacg ctgtattcct tcttcagaac
    28621 tgacatctgc ttgtacagag actgcttttc cgcctgcctc ttcgattgct ttgactgctt
    28681 tttctgctcc ttctttatcc gagttatagt tgacaactac attcattttt tctttcccga
    28741 atcgttgagc aatcgctgtt ccaatcccct ttgagccacc agttactact gcgactttgt
    28801 tttctaattc cttgtacata gtacccactc ctttttcata cctaaacctg ttattacgga
    28861 taacttttat ttccgcctta aagtgagtgt acaccaagat atatagtcta aagcaaatga
    28921 tacgctctag caatcgtttt catgaattgc tagtgtcctt ttatttctat aaagaaagag
    28981 gttgtgacag aagtggtcag cttcaagaaa tttcggctta tttccgaaga agctacttct
    29041 gaaaaaccat ttatttggaa aaaaaggctg tgacaagact tttgtcacag cccctttcct
    29101 gggcttattt atcacccatt ttattgtaat tgctagttat acataaacaa gagattttat
    29161 gcataatgtt ttattgctga gaaaaatatt cagctgttgc ttgttcatcc tccttgagtt
    29221 gttcaatcaa ttcctctaca ctagaaaatt tcacttgatc tcttaaaaat tgattccagc
    29281 gtaccgcgac atgttcacca tagatatctt gattaaagtc caaaatatac aactctacag
    29341 tcagctcccg tccttcaccg aatgtatcat tatgtccaat tgaacccatg gctggatacc
    29401 atgtttcccc aatttttatt tccgacacat aaacaccttc tttaggcaaa tgaaccgtgc
    29461 tctttacttt gacatttgct gtagggaatc ctagtagtct gcctcgtgca tcgccatgga
    29521 caactacccc ttctacctca tatgtgcaac ctaatagttt tgctgcttct tttacttgtc
    29581 cgctgtctag taatccacga atacgggtag agctgatttt ttcaccttgt tcttcttctt
    29641 tagaaactgt gacgattttg aatcttcctt tggcatattc aggaaaatga gcgacatctg
    29701 caatgtcttt tggtccatac gtataatcaa aaccggaaac agcatatttt gcatgtaacc
    29761 ctacgatgta ttgatcaaca aattcctgcg gtgctaaatg agcaaaagaa gaagtaaaat
    29821 caacttcata taaataatct atacccaaag cggccatttt ttcttctttt tgcttaaggc
    29881 tagtcaaata tttcacgctg ctaggatcga tctttttaaa gacaatagat ggatgctgat
    29941 tgaaagtcat caatgctaat ttcaatcctt ctttttgtgc aatttccata cctgtcttta
    30001 tgactttctg gtgtccgata tgcacgccgt cgaaaaatcc cagcactaaa acgacatcgt
    30061 cttctggaat ttgttctgat tggtaaggat gtctgatctt gatgatattc attcgttatt
    30121 cctcatttct caatacttta ctaggtttta atttgttttt ttctttcggg tttggctgat
    30181 aaatactgac aacttttcct ttataaaaca aagcaatttc ttctgacggc atttctggga
    30241 gcgcaaatac agtataatca agtctcatac cgttttttac tttctgccat acttcatcag
    30301 taatatctac tcgtttgaac cgttccactc ctttttctat tggcagcaaa caatcatcga
    30361 ttgtcccatt tttcacagct tgagctactt cttctaatgt cagagcatcc ttctgggaca
    30421 taccggcact tgctgttcgg gtcagatcag acatgtgcgc agggtaacca agtttcttgc
    30481 ctgtatctac agctaatgtg cggacatagg ttcctttccc gcattctact tcgaagcgcc
    30541 aagaaatggt tcctgtcact tcgtctcttg tcaattcact tgtccggata aaacggtaaa
    30601 tctgagcctt tctaacagga cgctccactg tttcattatt gcgggcatat tcatataacc
    30661 gcttaccgtt gacttttacc gctgagtaca ttggtggtat ctgggtgatc tcaccaataa
    30721 atgaagccat cacttcatct attttttttt cagataaaga catatctact ggttttgttt
    30781 ctacccgttc tccggatcga tcttccgtcg ttgtactgaa acctagagtt atctcgccta
    30841 catagatttt cccagaatct gtcaaatatt cgataacttt tgtggctttt ccaatacaaa
    30901 tgggcaacac accgtctacg tctgggtcaa gcgtaccccc atgtccgatt tttttcgtat
    30961 gtaagatttt tcttaatttg aatacgcaat catgacttgt cattccgcgt tctttccata
    31021 aaggtaataa cccgtccatg tcttttctcc atttcatttt tgatttaact gaattattat
    31081 aacacattgc atcaattact gacgaatttg acatcataat gaacggtttt tatttttcgg
    31141 caataaaaac gtaacaaaat tcatgctcac gtaaatagaa atcatgttat gcttttttaa
    31201 aggagggaat agaatggaag attttttctt tgcaccattt gattggattt atgggtggct
    31261 tacaggtaaa tagtttggtt ttcacttccc aaaaacgaaa aaaaagactg gccattcttc
    31321 cagtcttttt tccgtttttt ttatttattt tttcagtttc ttaatcaatg taaaaaattc
    31381 tattttgcgg gatcgatttt atcattttat tggtcaagat ttttccattt ccaatccaat
    31441 acttctggca gatcaatacc attttcacga atatagtttt gatgttcttg taatttctga
    31501 tccatttttt tcgcaaaatc ttctgcttga cttcctttga tttttaaagc tgcgtctttt
    31561 gccaaatgat aacgatcaag ttcatttact acacgcatgt caaatggtgt cgtgatatct
    31621 ccattttctt tgtagccatg aatcattaaa tgatggttat gacgatcaaa gaaaatcgtg
    31681 cggaccaatg tttcataacc gtggaaagca aataagatcg gtttgtcttt tgtaaaatat
    31741 tgatcaaatt cttcatcgct caacccacgc ggatcttgac ttggatgacg taatttcaat
    31801 aaatctacga cattgatgaa ccgaattttt agttttggga attcttccag cagcaaagtt
    31861 acagctgcta atgcttctaa gttagattcc gttcctgcag cagcaatgac caattcaggt
    31921 tcctcctctt ttgctgtcga agcccaatca atgattttca agccttcatt cactaagacg
    31981 gctgcttctt ctgctgagta aaattgagca cgcggatgtt tggaagagat gatcaaattg
    32041 attttttctg acgagcggaa tgctttatcc atgactgcga gcaatgtatt tgcatccgca
    32101 ggcagatatt cacggatgaa ctccgccttc ttttctgcca aatgtgtcaa tattcctgga
    32161 tcttggtggc tatatccatt gtgatcttgc tggaagactg ttgagcttgc aatgagattt
    32221 agcgaaggat aatcatttcg ccaagaaagg tcatgtgatt tccgcatcca tttgaagtgc
    32281 tgagtcaaca tagagtcaac cactcgcaag aatgattcgt aacttgcaaa aaaccatgcc
    32341 gtccagtcag aacgtaccct tctaagaacc cttcatcttg gtgttccgat aattgactgt
    32401 cgatgacgcg accagatggc gaaagccatt catcattttc tggttcgatt ttttccatcc
    32461 attggcgatt tgtcgttttg aaaacttgat tcaatcggtt tgattttgtt tcatctggtc
    32521 caaatatacg gaaattttct ggatttttct caatgatctc gcgaacgaat ttaccaagtt
    32581 cggtcatatc ttctgcttta acggagcctg gtttttcaaa ttgcaaagta tattttttcc
    32641 aatctggcat aataagcggt cttggatcga ttccaccatt ggtgatagga ttttttgcca
    32701 ttctttggtc accagtaggc gcgatttctc tgatttcttc taaaatacgt ccttgtgcat
    32761 caaataattt ttctggttca taggatttca gccaacggag taaagcatct gcatgatcca
    32821 tatgttcttg atctacagga atcggtactt ggtgtgctcg gaaggttcct tcaattggtt
    32881 ccccgtccca ttcttttgga ccggtccatc cttttggtgt gcgtacgatc aatactggcc
    32941 attttggcat cacgacatct gttgctgtct tcaggcgagc ttctttttgg atggatttaa
    33001 ttttctcgat tgcttgatcc attttttcag ccataacagg atttaatttt tctgaatcat
    33061 ttccttcaat aaagaaaggt tcccagccaa gtccgttaaa gtaatttgct aattctccat
    33121 ctgatttacg tgccaagatc gttgggttgg ctatttttgc accatttaaa tgaaggatcg
    33181 gtaaaacagc tccatctatc actggattga taaagacatt tgagaaccag cttccagcta
    33241 aaggaccggt ttctgcttca ccatcaccaa tcacgacagc agagattact tcaggattgt
    33301 ctaatacagc tccgactgca tgtgaaagag agtatccaag ctctccgcct tcatggatag
    33361 aacctggtgt ttctggtgct gcatgagaag caatgcctcc aggaaatgaa aatcgtttga
    33421 ataatttttg cattcctgtc tcatcttccg ttacttctgg atagatctct gtgtaacttc
    33481 catctaaata agcattcgat accatgactt gtccgccgtg tcctggccct tcaatataaa
    33541 acatgttcaa atcatattta ttgatgactc ggttcaaatg gacataaata aaattttggc
    33601 caggaatcgt tccccaatga ccgattggat gttttttgac atcttcgggt ttcagtgtac
    33661 gtctcagtaa ggggttatct ttaagataaa gttgcccaac agaaagataa ttagcagcac
    33721 gccaccaagc cgtcatttta tcaaagtatt ctttactaga ataatccata cataacactc
    33781 cttttcatta ttactcttat tctagcgcag atactcccaa ttgaaaccta ataagcactt
    33841 ctgttcattt gtttggattt tatatctagc caaatgaaca ggtataaaaa accacgaaat
    33901 ttttcctttc gctgacgttt tttacattga tccaaaaaac ttgtcgttat cacaatggct
    33961 gttccgcagt tcatttcgct cctcatcatg cataatttgc tgaatgacgc tgggccagtt
    34021 aacacattgc tggaaaactg gggctggatc gagcaattcc tttcctgatt gatccgtttt
    34081 atgtaaaagt ctctgttatc tttgtcaata tgtgaattgg tatcccagct actatgctaa
    34141 tttctacagg gattatccaa aatttggatc gtgaccaaat cgaagcggcg taatcggaaa
    34201 tatcaataac ttcaacgtga tttttctttt aactgtcaac ggaccgacaa actcaaactt
    34261 ttatggtgca ggttccactg atcttctagt tacttggcta tacctgactg tcactaaaat
    34321 agattacaat ctagcttccg ttatcggtat cttgatcttt atctatatgc aaaaatttat
    34381 atactactag aaaaaagaaa gtcgaatttt ttacttttct ttttctcttt tttggttagc
    34441 tgacacgccg caagactaaa gtcatggaac tcttaggtaa tacgtcttaa tgaaaacaaa
    34501 aaaatcccgc aacccctgat ttcacaggat ttgcgggagt ttttgattag tctttgttca
    34561 agtcgcgaag caattcatcg atatggttgc catagacaac tgattcatca agttcaaaga
    34621 acaattcagg tgtcttataa atacttagat tgtgccctaa ctctcgacga atcaatcctg
    34681 atgctttatt caatccatct tgtgctgttt tcttatctga tgctaagtca gataaaatgc
    34741 tgtaatagat cgttgcttgc tgtaagtccc ccgtaacttt cacatctgtg atcgttacgt
    34801 tctccacacg agggtcacgg acctttttgc gcaaaatatc attgacttct tttaagattt
    34861 cttgaccgac tcggcggtca cggtagttag ccataatgat tggcctcctt ttatcagttt
    34921 ttagtcattt ttgacttctt ccatgataaa tccttcaatt acgtcatcta ctttgatgtc
    34981 attgaatttt tcaatcattg caccacattc ataacccatt ttgacttctt tcacgtcatc
    35041 tttgaatcgt ttcagacttg caagttggcc ttcatagatt acgattccgt cacggattac
    35101 acggacaccg ctatctctac gaatatatcc ttctgttaca taggcacctg caatcgtacc
    35161 aactttagaa actttgaagg tttcacgaac gatcatttga ccggtgattt tttcttcaaa
    35221 ttcagggtct agcatccctt tcatcgcagt ttcgatttct tcgatggctt tatagatgat
    35281 acggtgaaga cgaatatcga cttcttcagt atcagcttgc acttttgcct gcggtgtagg
    35341 acgaacgttg aaaccgatga taatagcgtt gctggctgct gctaaggtta catcactttc
    35401 attgattgct ccgacagctg aatggacgat ttttacacgg acaccttcca catcaatctt
    35461 cttcaatgat gcagccagtg cttctgctga accttgtaca tctgctttga tgattacgtt
    35521 aacttctttc aattcgcctt ctttcaggct ttcaaataag ttatccaaag tcacacggct
    35581 tgtggcagca cggttttcta ataatgcacg tttcgcgcgt tcttcacccg ctgcacgtgc
    35641 tgtcttttcg tcttcgaaga ctacgaaacg gtcacctgct tgaggtacat cattcaagcc
    35701 agtgatttct actggcgttg caggtcctgc ttctttatcg cgacggccaa gatcgtttgt
    35761 cattacacgg acacgtccgt aagtattacc gacaacgatt gggtcaccaa catgaagtgt
    35821 tccttcttgg acaagtaagg ttgacacagg acctttacct ttatcaagac gtgcttcgat
    35881 cactgttccg attgcgcgtt gtgttggatc tgcttttaaa tcttctactt ctgcgactaa
    35941 caggatcatt tccagcaatt cttcgatgtt ttggccgaat ttcgctgaga tctcaacaaa
    36001 gatcgtttct ccaccccatg cttcaggaat taactcatac tcacttaatt cttgcataac
    36061 atgttgaggg tttgcacctg gtttatcaat tttatttaca gcgacgatga ttggaacgcc
    36121 tgcagctttg gcatggttga ttgcttcaac tgtttgtggc atgacgccat catctgcagc
    36181 aacaactaag atcgtgatat ctgtgatact tgctccacgt gcacgcatac ttgtaaaggc
    36241 cgcatgtcct ggtgtatcca agaaagtgat tggtttacca tcaatatcga tctggtaggc
    36301 accgatatgc tgtgtgattc cgccggcttc gccgcttgtt acacgtgaat ggcgtaatgt
    36361 atccaataaa gttgttttcc catggtctac gtgtcccata atcgttacga ctggcggacg
    36421 tgagactaat ttatctggat cgacttcttc tgcctcgaag aatttatcga tatcagcaat
    36481 gtcaacttgg actttttctt gtggttccat gccatagtcc gtcgctaaca gttcaatcgt
    36541 atctttatcc agtgcttggt tttgattgac catgacacca agcataaata attttttaat
    36601 gatttctgct ggctcacgat gaattttttt cgcaatatct gctacgttca taccttcagt
    36661 atactctaat acatctggca attcacggaa tttgcgtggt ggtacagctg gtttagttga
    36721 ttgttgctgt ttgccttttt tacctttttt attataacga ttacggttat tattattaaa
    36781 tttgccgcga ttgttattgt ttggtttgtt gttattgtta gtacggtttt gattgccgcc
    36841 ttggctcgtt ccttgacgat tttggctgct ggtatttccc gtattatttt ggtttgttgt
    36901 tggttttttt ccttgattca cttgaccgct cccttgaccg cgatcttgat aactgcggtt
    36961 atttttattt ttttgttgat tgttcgttgt ttctttttta cgttgatcgt tcggctgttg
    37021 attggctggt tttctgcttg cttgttgtgc agcttgttgt ttcgtgcttg ggtttttgaa
    37081 cgcttgttgc aatttcttct catcattgct agtaatcgca cccatatggt ttttcacatc
    37141 gatgccaagt tgttgtgctt tttccacgac gtctttacta gattttttga gttcttttgc
    37201 taattcatag attcttttgt tgcccatgca atcaccttcc taaccttcaa ttagctcctt
    37261 ggtcttcttc gcaaatccgg catccattat tccgaatacc ttgcgaggtt ttccgatcat
    37321 ctgcgtgatt tctgcttcag aaaatagctc aaagcaagga acttcataat atgaactctt
    37381 atcttttatc ttttttcttg tattctcgct tgcatcggtt gccacaaaca ccaattttgc
    37441 tttttgtttg cggatttcag caatggtcag ttcttcaccc gtgattagtt ttcccgcgcg
    37501 catcgctaga ccgatgagat tcatggcttt ttgccgatta actgtattca ttttccaaat
    37561 aactctttac gtgctttttg gtgagtgaca tagtcaagaa gttcttgata aaactcatct
    37621 gttaatgttg tttcgagtac tcgatcgaga atttttttat cccaagcttg ttgtacttct
    37681 tccggctcaa gtgagacata cgctcctcgt ccaggcattt ttccagttgg atcgatcgat
    37741 acttcccctt cttttgaacg ggtgattcgg accatctctt ttttaggttt catttcacct
    37801 gaaaccacgg atttgcgtaa aggaattttc ctttgtttca tctctttgcc tccttactaa
    37861 agttaagctt ctggtttttc ttcgaccgtt tcattgttga atgcaattgt ttcatattca
    37921 tcgtctgtta aatctgactg aacgattgct tcatcatgca gttcttctgt ttctttttgt
    37981 tgttcttgat tttcataaaa ttccgtcatg tcagattctg atttgatatc gattttatgg
    38041 ttagtcaatt tagcagctaa acgtgcgttt tgtccgcgtt taccgattgc tagcgataat
    38101 tggtagtctg gaacgacaac tgtgcaggcc tttggatttg tctcatcgaa gatcacatcc
    38161 atgacttgtg caggattcaa tgcatttgca atgaatacag ctggatcttc attccattcg
    38221 acgatatcca tgttttcgcc ttttaattca ttgacgattg cttgaactcg ttgtcccttt
    38281 ggcccgacac atgtaccgac tgcatcgata tttggatcat ttgagcgaac tgccactttt
    38341 gaacgatcgc cagcttcacg ggcgatgctg acaatttcaa cgattccatc atagacttct
    38401 ggtacttctt gctcaaataa gcggcgcaat aggtctggat ggctgcggct aacaaatact
    38461 tgcggacctt tagacgtatt ttctactcgg gaaacataaa ctttgattcg atcatgtggt
    38521 tgataaaatt catttggcat ttgatcttgt ttagaaagaa ctgcctcaat tttaccaaga
    38581 ttgacataaa tataacgttt atcctggcgt tcgacgattc cttgcatgat gtctttttca
    38641 taagcactga attcgttata aataatcgtt ctttctgctt cacgtacacg ctgtaaaata
    38701 acttgtttag ccgtttgcgc agcgatccgc ccaaaatctt ttggtgttac ttcaaaacgg
    38761 atcgtatcac cgatctcata tgctggattg attaaaagtg catctttcaa tgagacttcc
    38821 aactgtgaat ccatcacttc ttccgttact tcttttaccg catagacatg gatctttcct
    38881 tttttctgat caaattccac ttcgacgttt tgtgcttggc catagtgacg tttgtatgca
    38941 gaaaccaatg ctgcttctaa agcatcgatc acgatttctt ttgagatccc tttttcagct
    39001 tctaaagcgt ctaacgcatt taacatttct ttactcattt tttctgattc ctctccgtga
    39061 ttaaaattgg atagctaagc gagcttttgc aatatttttt cggtcaaaag tcaattcttt
    39121 ttctcgtgtt tttatgcgta ttttcaatgt aagctgttca gcatcaaatg attgaagaaa
    39181 tccttcatat tgtttttcgc catctactgg ttggtataag gagacatgga cataatcacc
    39241 aagtgccttt tcatagtcgc tttctttttt caatggccgc tctgctcccg gggaggatac
    39301 ctccaggaaa taagcttgag gaatcggatc tggatctgct gcatcaagtt tttcactcag
    39361 tttctcactg acaaaggcgc attcttcgat atcgataccg ccttctttgt caataaatac
    39421 tcgtaagaac cagctttttc cttcttttac aaattcgact tcgacaagtt caaaattttg
    39481 ttcatctaaa atcggtgtca ccatttctgt gactgtttca acgacgctac tcaaaagttt
    39541 cgcctcctta aaaaattaag tgcgtaattc cattaaaaag agtgagcgaa aaatccgctc
    39601 actcacagtt agtatcatta cctataagaa ctataccata ctctgcttaa aattacaagc
    39661 agacaggaaa acagatgctt atagcatatc gaataacgat aattgatttt catctggtag
    39721 atctttcaat acgccattgt cgttcatata ttcgatcaat gtttttgaaa ctttaccgcg
    39781 ggtggcaaga tcttctttgg ataaaaatgg accgtctttt cttgcttcaa cgatttgttt
    39841 tgccacgttg gttcccaaac ttgggacagc tctaaatggt gcgatcaacg tatcaccttc
    39901 aataacaaag ttaacggcat ccgatttata caaatcaatc attccaaatt tgaatcctcg
    39961 ttccagcatt tcattggcta actccagcac tgtcagttga ttcttttctt ttacggaagc
    40021 atctaatcct ttgtccgtga tttctttcat tgcttgtttc acagcatctt ttcctttgca
    40081 catggagact agatcaaagt cgtctgcacg tacagaaaag tatgcacagt aatacaaaat
    40141 agggaaataa accttgaaat aagcgacacg aagagccatc aatacgtaag ctgcagcatg
    40201 ggctttcgga aacatatatt tgatttttga acaagaatcg atataccagt cgggcacatt
    40261 attttctttc atcgccgata gataggtttc tcgcaattca tcagggatct tgttccattg
    40321 tcctttacga acagtttcca tgattttaaa tgccatcccg ctatctaatc cagcatggat
    40381 caaataaacc ataatatcat cacgacagcc gattacttcc gccagtgtcg catctcctcg
    40441 tcgaatcaat tcttccgcat ttcctaacca tacgtctgta ccatgagata gtccggaaat
    40501 ttgcagtagt tctgcaaaag ttgtaggatg cgtttcttct agcatccctc gaacaaaacg
    40561 agtgccgaat tctggaatac ctaatgtccc tgttttggaa taaatttgtt cctgacttac
    40621 tccaagtact tcaggaccgg caaatatccg catcacttcc gggtcatccg tagggatcgt
    40681 ttgtggatcg atccctgata aatcttgaag catccgaata acagttgggt catcatgccc
    40741 tagaatatct aatttcaata cattatcgtg aatcgagtga aaatcaaaat gagtcgtttt
    40801 ccattccgaa ttttgatcgt ctgctggata ttggatcggc gtaaaatcat agacatccat
    40861 ataatcggga ataacgatga tcccccctgg atgctggccg gttgtccgtt tgactccagt
    40921 agcgcctttt gctaatctgt cgacttctgc acttcgaaac tgcagattat tgtccctttc
    40981 gtatcctttt acatagccat atgctgtttt gtcggcgact gtaccaatcg ttccggcacg
    41041 gtaaacatag tcttcgccaa acaatacttt cgtatagtta tgggcttctg cttgataatc
    41101 acctgagaag ttcaaatcaa tatcgggcac tttgtctcca tggaatccta aaaatgtctc
    41161 aaatggaata tcatgaccgt ctttgtttaa gcgtgcacca cattttggac attgtttttc
    41221 tggcatgtca aatccagaac catacgtacc atcttcaaaa aactcggaat attggcactc
    41281 tggacaataa taatgagggg ctaacggatt aacttcagtg attcctgtca tcgtcgcaac
    41341 aaagctagag ccaacagagc cacgtgaacc tactagatag ccatcttcat tacttttatg
    41401 cactagtttt tgggcgatca gataaataac cgagaagcca ttaccattaa tagaattcaa
    41461 ctcttttttt aatcgttttt gaatgatctc aggtaacgga tctccgtaca tttgttttgc
    41521 ttttgtgtaa ctcaattctg atatttcatc ttctgaacct ggaatcttcg gtgtataaag
    41581 ttcatctttg actggaatca cttcttcgca gatatctgcg attttattcg tattttctac
    41641 tacgatctct ttcgctgttt cttcccctaa aaagtgaaat gcagttagca tctcatcggt
    41701 tgttcggaaa tggacatccg gcaaactatg acgattcaac ggattcgctc cgcccataga
    41761 attgatcaaa atttttcggt aaatggcatc ttcttcgttc agataatgta cattacctgt
    41821 tgcaacaacg attttatcca aactttttcc gatttctacc aaattttgga taatttcttc
    41881 caaatcatgt tcatttttta ccaattcttg ctcgatcaaa ggcgcataga ccgcttttgg
    41941 cataacttca atataatcgt agaattttgc tcggtttctt gcctcttcta cccctttttg
    42001 catcatggct tcgaatattt ctcccttatc acaagctgag ccgatcaata gattttcacg
    42061 catctttttc aattgggagc gtggtattcg tgggacgcgt tcgaagtatt cgacattcga
    42121 cattgaaatc agtttgaaca aatctttcag tcctgcctga ttttttgcta aaatcgttac
    42181 atggaaaggt cgagcccttt tgtaagaatc tccttctccg atatgctcat ttagctgatc
    42241 atgataaagc atgttatgat tgtccattgc ctctttcaca aagatccatg ctaagtgacc
    42301 agttgcttca gcatcataga ttgctcggtg atgctgttcc aagctgacac caaatttttt
    42361 agataacaca cctaatccga aacgtttgaa ttgaggatac agatatctag ctaattccaa
    42421 cgtatcaatg actggattgg ccgcttcagg aataccatac cttgcgtagc tagtattcaa
    42481 gaaccccata tcaaaagccg cgttgtgggc taccaaaatt gtatcttttg aaaattcaag
    42541 gaacatccgc aatacttctt cttccgactt tgatcctcga accattccat ccgtgatacc
    42601 agtcagatct accgttgttc gtgacaaagg atgtcctgga tcgataaatt catcaaagct
    42661 ttcgatcaca tttcctttat acattttgac tgcggctagc tcgatgatcg tgtcatatac
    42721 tgcagacaat cctgtcgttt ccacgtcaaa tacgacatat gtggcttcac tcaatgcttc
    42781 atgtgcatca ttataagcaa ttgggacacc atcatctacg acattcgctt ccacgccgta
    42841 taagattttc acacctgctt tttttccagc actatgtgct tccggaaagg cttgcgcacc
    42901 accgtggtct gtaatggcga tagctcggtg tccccatttc cctgcttgtg ccacaagatc
    42961 agagatgctg tttgttgcat ccatcgtact catattgcta tgaacatgaa gctctactcg
    43021 tttttcccct tcaggagcat aatctttgcg aggtgtatgt ttgacttcta tgatatcttg
    43081 cgcattcatg accaaatcac ggacaaacgt atcttcttga atacttcctc gtacctttaa
    43141 ccagctaccg acactgatcg catcaaatac ttgttcatct ttttcgccgt tcgagaattt
    43201 tttgacgata aatgatgaag tataatctgt gattttcaaa gtcaagatct tgcgttttga
    43261 acgtagttct ctgacttctt tgtcgaatac gtacccctca agtgtgactc ttcgttcttc
    43321 ttcaacgata ctgatcattg gtgtcgttgg ttcatcggct ggaatatttc gtcctagctg
    43381 gatgtggatg ggtccttcaa gagctggcga ttgctctttt ctttcctttt tcttttgttc
    43441 gtggacaact agactttcag ctgcttgctt cataaaggct tctgcctgtt cttgtttgcg
    43501 ttcttcaaaa agtttcaata ctcgttccgc ttgctgctca tccacttctg gttcgatacg
    43561 aaattttggg aatccgtaac tgacgaacaa ctcttctact aatggcaagt attgttgctt
    43621 caaataagaa atagctcctg tactgtcgat tggcaagatg actttacgat cttttatcac
    43681 aggtacctgc gttttcaata cttgctgtgc tagtggtgta tcgcattgct gattttccaa
    43741 agcttgggcc cagtaatctt gcaagagctg ttcatcgaat gtctggtcat ctgctaagat
    43801 attcagtttg acttgtgcaa tatctttaaa agcgatagtc agatgctgag tcaatgaccg
    43861 atatagcata ataggcaaaa ttttggaaaa atgcagcgta aattcccata aacgcgattt
    43921 acgatggacc actacactat ccatttcacc agcagtaagc agtggatgat ttttttctgc
    43981 ttcctctagt tggatttgtt ccaacagttt ttcaaataaa tctcgttttt ctgacaaaga
    44041 tatacgccct ttctgatgag aaacaatgca attgatcgca tcttatcctc ttctttttca
    44101 gtacctttta gtatactcgt tttttctgag ttttacatta aaaaattgga actgcgattc
    44161 aaatcgcagt tccaatttta aaagcgaatc atcagattgt caaaaacagc tgattcagct
    44221 atttttattt tctgtgtttt aacgcttatt attcgccttg gttcaagagg attggtaaag
    44281 tattcaccaa ttcttcttta cgtacttcaa ccatttcgcc agttttcttg attttcactt
    44341 ctacgattcc atccactgct tttttaccga cagtgattct gattggacag ccaatcaaat
    44401 cagaatccgc gaatttcact cctgcgcgtt catttctgtc atcaaccagt acttcatagc
    44461 cagcttttgt catcgcttct tctagttcat tcgtcaggtt tgtttggtat tcgtctttta
    44521 gattcatttg gacgatatgc aaatcaaatg gcgcaattcc ttttggccag ttgattcctt
    44581 gttcatctga atgttgttct acgattgctg ataataaacg acttacaccg ataccataac
    44641 agcccataat cacaaatttt tctcgtccat tttcgtctag tacagttgct cccatagatt
    44701 cactgtaacg agtgccgatt ttgaagatat gtccgatttc gattcctcga gtaaaggcaa
    44761 gaacaccatt gttgtctggt gaaggatctc cttcttgtac aaaacgaaga tcttcataag
    44821 aaagtacagt gaaatcgcgt tctggattca catttttata gtggaatcct gtttcgttcg
    44881 ctccagtaat ggcattcgct aaatcttgga cataacggtc tgcatatact tttacgtcat
    44941 ctgaaacacc gactggtccg acagaaccaa actcagcacc tagatatttt tgtgcttctt
    45001 cttctgttgc ttcttctaaa aagtcagcag ataaaaagtt tttcagtttt acgtcattga
    45061 cttcatggtc tccgcgtacc aaaacaagga caggttgttc atccgcaatg aacaatacag
    45121 acttgatgat tttttgaggt tcaacagaaa agaaatctgc tacttcgtca attgatttta
    45181 cgttaggtgt agctactttc tccaattcaa gctgtgtttc atgcgatttt tttgatacat
    45241 agtagcttgt agccatctcc aagtttgcag cataatcact ttcagtcgaa taacagatcg
    45301 tatcctcccc gatttctgag atcgccatga attcttttga gtctttcccg cccattgcgc
    45361 caccgtctcc gataatcgag cgaaattcta agccacaacg cttgaatact tcggtgtagg
    45421 ctttttcgta atctttatat gtctcgtcca aactttcttc cgttgcatgg aaagaatacg
    45481 catctttcat gataaactct cttccgcgaa gcaaaccgaa gcgaggtcgt ttttcgtcac
    45541 gatatttagg ttggatttga taaagattta aaggtaattt cttatatgaa ttgatttcat
    45601 tacgcatcaa atcagtaaaa gtttcttcgt gagttggtcc aaggatcatt tttcgatcat
    45661 tgcggtcttt gaaacgatac aagtttggtc catatgtttc gtaacgcccg gattcttccc
    45721 aaagttctgc tggcaagatg gcaggcatca gcatttctac cgctccgatt ttttcaaatt
    45781 cttcccgcat aatgcgtttc agtttttcca atacacgatt tgctaatggc agatatgaat
    45841 aaatacccgc tgatacttgg cggatatatc cagctcttag caagatttga tggcttaata
    45901 cttctgcatc atttggaact tcgcgtaatg ttggaatcaa catttttgat tgtctcattc
    45961 tatatactcc tttatttcct caaaatagtt atcgtttgtt attataccct atttgtcaac
    46021 tttttctaga aaaagaatcg ttgaatatcg ttccatgtaa ctagcaccat caacaacatt
    46081 aaaaatccaa agccagctaa tgtcaatatt ccttcttttt cttgacttaa tggtttcccg
    46141 cgaatccctt caaaaatatt caagactaat ttcccgccat ctaaggcagg aatcggaagc
    46201 agattgacga ttccgagatt cattgaaaga agcgccatca aactaataac agttgttaca
    46261 ccttgatttg ctgcttctga agacaattgg tacatcatga caggtccgcc taatttatct
    46321 aaactgaaac ctgtaaatag tgatcctaag gctttgaaaa tttccaatga accgctgaaa
    46381 gcttgacgcg tccctccaat gatcttatcc ataaaacctg tattcattgg ggcttttata
    46441 ccaagttggc caactttttc gccatttgac tctacagatt tcggtgtcac gtcgacagat
    46501 tgcacctgac cttctcgttc gatcttaaag tctaatgttt tatccggatt tttcgtaatg
    46561 actgttgtca gatcattcca tgaatggatt tcttttccgt ccacactgac tacctcatca
    46621 ttttctttta agcctgcttc tgccgcagct ccattaggca tgatctctcc tacgcgatta
    46681 gtgttcgtca cttgaacacc accttgcata aatgccagaa tgatgaataa caggattgcc
    46741 aaaataaagt tattcatggg tccagcaaag ttggtcagca tacgctgcca caatttagcc
    46801 gattggaact gtacgtcttt tggcgcaatt ctgatttctg ttccgtcgtt ttcaataatc
    46861 gtcgcatcgt gatcaacagg gtaggttact acttctgttt cgtctccatt cacataacca
    46921 gtgatagtca gctcatcttc taaatcataa cggctcaatt ccatcgggat agcatttgtc
    46981 aattggattt tcttacttaa atttatcttc tctacgatgc cgtcagaatt tagtaaaaga
    47041 gaaagaggca ttcctggtgc catttctgtt tcatcatccc cattgcctgc cattcgaaca
    47101 taaccgccaa taggtaatag gcgaagcgta tatgttgtcc cgtcttttgc ttgatgccca
    47161 taaattttcg gacccatacc gatagcaaat tcgcgaacga ggattcctga tcgtttcgca
    47221 aagaagaaat gaccaaactc atgaacaatc actaatatac caaaaacgat gataaatgtc
    47281 agaatcgttt tcatagcttt tttctccttt acttgttttt tgtgtttttt ataaaacaac
    47341 gataaaacac tataatttta ccatagtata ccaaagacac caatagtatc aatataatga
    47401 aaaataaaaa cctgcgtctt ttatgaaatt cacaaagaaa cgcaggtata cttactaaat
    47461 aatgccaaat aaatgcatga taggaaatac aaacagcaaa ctgtcgaaac ggtctaagat
    47521 tccaccatgg cctggtaaga tatttcccga gtctttcaca tcataatgac gtttgattgc
    47581 agattcgacc aaatcaccaa attgtccgac tgctgaaaaa ataatcgtca tcagaagcat
    47641 gacaaacata ccatgattaa atagttcttt tgctgggtat agcaataaat aaagaaatgc
    47701 gactacaaca gcactaccaa taccaccgag cgcaccttca atcgttttgt tgggcgaaat
    47761 atcaggactt aacttatgct gaccataacg tcgaccaacc atatatgctc cgatatcagt
    47821 tgcccaaaca ataaatagac caaatatcaa aacagccaag ctttctgttc gtgcattgac
    47881 aaaattctgg aaacctattc ccacatacaa gctgacaagc acaggaaaac ctgcttcatc
    47941 aatcgtatac gtatttttag aaataactga tgttcctaaa agaatcataa ctataagata
    48001 aaataacata aatgtactta cattctctgg aagaaagaaa aaccatcttt cttttggcaa
    48061 aacaagtaac actgcaccaa cggcggataa aactccttca aaacttaaga gtgtcaatcc
    48121 tttcatccga aacagctcgt aaactcctat tactgctaag attgctgctg taagctcaac
    48181 cgctatgcct ccgatccata taatcggaat aaacaagact agcgccacgg ctgctgtgac
    48241 cacacgttgt ttcatgactg atcccctttc tcatcttctg ttttttttac accgccgaat
    48301 cgacggtctc gattttgata agaggcgatt gcttcttcaa gatgcgcacc atcaaaatca
    48361 ggccaaagcg cttttgtaaa atacagctca ctatatgcga tctgccataa cagaaaatta
    48421 ctaattcgct cttctccact tgtacggatc atcagctcag gatcttgtaa ctctttaggc
    48481 aagaaacctg tcattaaatg cttagcaatc aattcatcat cgatttcttc tgcgtgtatt
    48541 tctccttttg ctacttcttc ggcaatttct tggacagcag tcacgatctc tgctctgctt
    48601 ccataattca aagcaaaatt caataccatt ccagtattgt tttttgtttg ctcaattgca
    48661 cgtcttacag catcttgcgt atgttctggc aatacgtttt cataacccat gacatgaact
    48721 ttgacatttt ctttaatcaa ttctggtacg aacgtatcaa agaagtcgac aggtagttgc
    48781 atgagaaaac ttacttcatc ttttggtcgc ttccagtttt cagtcgaaaa ggcataaagc
    48841 gtcaacactt tcactccaag ccttgatgct ttttttgtga ctttctttac tgtttccatg
    48901 ccttctttat ggccagcaac tcgtggcaaa cgacggtttt gcgcccaacg accattgccg
    48961 tccatgatga tggcaatgtg ctgaggaatt ggtccatcgg tattaaattg aaattcgctt
    49021 ttttcttgaa tatatttgtt tttttgcgga aaaaaacgta acattctttt tcctcctata
    49081 cttaaaagat tcccactatc ttaagtatta tagcaatatc taactgtttt tcctatgaaa
    49141 tcattgtttt tttatttaaa aactgaataa ttttttcttt ttttaatttt taaataaaag
    49201 aaatataaat aaaaacagag gaagcggaat gatccgcccc tctgttttta tgattttatt
    49261 aaacttccaa taattctttt tctttatcag cagcgatctt atctacttct ttgatgctgt
    49321 tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa atcatcttcg gtaatatcac
    49381 catttttttg ttgtttttta tattcatcga tcgcatcacg acggatgtta cgaatcgcga
    49441 tttttgcatt ttctgcttct tttttaacat ctttagctaa ttcttttctg cgttcttctg
    49501 tcagttgcgg gataactaag cgaatgacat ttccatcatt ggttggactg attccgatat
    49561 cacttgccat aatagctttt tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca
    49621 ttaaaacacg tgcttctgga atttggattg atgccatttg gttcaatggt gttggcgcac
    49681 cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt
    49741 gcccaagttc acgttgaaga ttttgtgctg cttttttcat tttttctttt gcttctgtca
    49801 taattgcatc tgccattttt atttccccct tacagttgtt ccgatatttt cgcctaagat
    49861 tgcacggcga atatttccgt gttcatttaa attgaagaca agcagtggaa tatcattatc
    49921 catgcttagt gaacttgcag ttgaatccat gacttgtaat cctttggcaa taacttccag
    49981 atgagtcagt tcttcaaatt ttactgcatt tgcatctact ttcggatcag cagaataaac
    50041 accgtctaca ttgtttttag ccatcaggat aacatctgcg ccgatttctg ccgcgcgtaa
    50101 tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta ccgcctgcaa agataacgat
    50161 ccgtcctttt tctaaatgac gttctgcttt acggcgaata tatggctctg cgatttgacg
    50221 catttcaatc gaagtttgca cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa
    50281 agctaaggca ttcataactg tagctaacat acccatgtaa tcagcttgag cacgttccat
    50341 gcccatttgt gctccgattt gtccgcgcca gatatttccg ccgcccacaa cgattgccat
    50401 ttcaacgccc agctcgtgaa cttctttgat ttcttggatg atttctttga ttactggtgg
    50461 tttgattcca aatcctgcgt ccccagctaa agcttctcca ctcaatttta agactacacg
    50521 ctgatatttc ggtgtgacca tgttcattcc tccaatattt tctattgcca ttttaccata
    50581 cttagatgat ttcgacactc taagactggt ttttcttgtt ttgcttaaaa aaagggagcg
    50641 cacaaaccgt gcgttcccgt ctgttattca acagaattat tttttcactt ggctcataac
    50701 ttcttctaca aagttatctt cgcgtttttc gataccttcg ccaacttcga aacgtacgaa
    50761 tgttttaaca gttgcgcctt ttgatgcaac gtatttttca acagtcatat caggatcttt
    50821 aacgaatggt tggtccacca aagcgatttc tgctttgaat ttcttcaagc gtccttctac
    50881 cattttttca acgatattcg ctggtttgcc ttcgttcaat gcttgttctg taagaacagt
    50941 tttttcatgt tctaattcag cttctggaat ttgtgattcg tttacataac gaggattgat
    51001 agctgctacg tgcatagcta cgtctcttgc aactgtttcg tcagttgttc catctagaac
    51061 agtcaatacg gcaatacgtc cgcccatatg caagtatcca ccaaatgcag cattgtcgtc
    51121 tttttctaca acttcaaagc gacggaagct gattttttct ccgataactt gagttgcttc
    51181 gatcaagtca gattcaattg tacctttatc tgttttgatt ttcattgcag cttccatgtc
    51241 tgctggtttg ttttcagcaa caagttctgc aatttctttt actaaatctt ggaacatttc
    51301 gtttttagaa acaaagtctg tttctgagtt tacttcaacg attgctgcag tgttaccttt
    51361 cacagcaact gaagccaatc cttcagctgc aatgcggtca tttttcttcg cagcctttgc
    51421 cattcctttt tcacgtaaaa gatcaactgc tttttccatg tcgccttcta cttcaactaa
    51481 tgcttttttc gcatccatca ttcctacgcc agtcatttcg cgtagttctt ttaccatttt
    51541 agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc
    51601 ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca gctccatcat tcaaaattat
    51661 tctgctgaag aattgtcgcc ttcaacaaca tctacgattt cttcgattga tgttgctgtg
    51721 ttttcttctg tgaaatcttc ttcaactact tgatcttcac cttggtttcc ttcgatgaaa
    51781 gcatcagcca tttttgaagt aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg
    51841 attactacgt cgatttcatc tggatcgcag tttgtatcaa ccatagctac gattgggata
    51901 tttaatttgt gtgcttcttg aacagcaata cgttctttac gtgggtcaac gatgtacatt
    51961 acatctggga ttcttggcat atcagcgata ccgcctaaga atttttcaag acgttcacgt
    52021 tctttgttca aaccagcaac ttctttttta ggtagaactt caaaagttcc atcttcttcc
    52081 attgcattga tttgtttcaa acggctgata cgtttttgga tagtatccca gtttgtcaat
    52141 gttccgccta accaacgatg gttaacgaag tattgtcctg cacgagttgc ttcttctttg
    52201 attgcttctt gtgcttgttt ttttgtacct acgaataatg ctacgccgcc ttcttcagca
    52261 acgctcttca tgtaatcata agctgcatct actaatttaa ctgttttttg caagtcaatg
    52321 atgtagattc cgtttctttc tgtgaagata tatttcttca tttttggatt ccagcgacga
    52381 gtttggtgac caaagtgtac gccggcttct agtaattgtt tcatagaaat tactgccatg
    52441 tttgtttcct ccgatttggt tttatttttc ctcttcttat cttctacctt gccggcaaac
    52501 tttttcaagc acctaccgac aatccaataa gaatgtggat ttgctgatgc ataaagcacc
    52561 ctcagctatt atacagacaa aagatccgta tcgcaagagc aaatatactt ttctgctcaa
    52621 cattcaatag tttacctgaa agataaaaag aattcaagaa aaacagtaga tttctctttg
    52681 tttctttatt atcctttttc cataaaataa ttttaggtat aaaagacgaa tattattggg
    52741 caatccttct tttttttgtt atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg
    52801 cgcaaagctc aaagacattt gctgattaaa caagtcattg aagaattccc gatccgtacg
    52861 caagaagaac tattaaacaa acttgggcat tatggcgtta ccgccacgca ggcaacgatt
    52921 tctcgagata tccgtgatct aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc
    52981 ttcgtattgt ttcgcgaaaa aggaactcct gagactagga ctgaggaaga ggatcggctg
    53041 gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc
    53101 aataccttgc cagacaatgc tcatttattt gcatctgttt tagatgagat caaacctccc
    53161 tctattgtca gtacaattgc tggattcgat acgatcatgg tcatctcgga aaatgaggag
    53221 gacgcaaaac gagtagaaga atttcttcac catccaactg aagtatcact tttttaatca
    53281 gagcacgtat tcctaatatg gaatgcgtgt ttttttattt tcaatatttt ccttattgaa
    53341 taaaccttta cactctttac aaaaatacat cattttttat tcaaattttt caacaccttg
    53401 attgaatcac aataatcatc atttatattg gatgttaaca agagcagctt cttgctcttt
    53461 gtgtatttca aaaaagaaat gaaaggaaat catatgtata aattattaac ttctggacca
    53521 ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg atcattgtac ttgggatcaa
    53581 gaatacaaag aaattacaca atggataaga aaaaaattat tggatcttgc tcaagtatcg
    53641 gaaggaatat acacaacggt cttaatgcaa ggtagcagct ccttcggggt agaatcggta
    53701 ttaagttctg tgatcaggcc agaagatact gtactgattt gttcaaacgg cgcatataga
    53761 caacgaatga tcaacatgtg cgaatgtctg aaactgaact ataccgttta tgaggtatcg
    53821 gaacatcaaa tcctccaagc tcatgtgatt acacagctga ttgaagagaa tccgtctatc
    53881 tccgcactag caatggtcca tagcgagact acttccggta ttttgaataa tttggaagag
    53941 attgctgctg tcacacaaga aagagatctt ttgtttatag tggacgcgat gtcaagcttt
    54001 ggtgggatac cgatttgtgt agccgaacta gggattgatt ttttgatttc cagctcaaac
    54061 aaatgtattc aaggtgttcc aggcttttct tttgtacttg ctaataaagc ggaactggaa
    54121 aaacgaaaaa atgttagccg aagtttgtca cttgatctat atgaccagta ccgcgtgatg
    54181 gaaaacgaga atggcaaatg gcgtttcact tcccctaccc atacggtttt ggcttttcaa
    54241 caagcgctga aggaactaga ggcagaagga ggaatcgaaa ctcgatattc acgttatctc
    54301 gccaataatc atgtgctacg acaaaaaatg acagagatgg ggttccagcc tttcattcaa
    54361 gaatatcagg ggccattcat cacctctttt ctttacccta aggactggcg atttgatttt
    54421 aatcattttt atcagtattt aaagcaaaat ggatttgtga tctatcccgg gaaaatcagc
    54481 gatcatgaat gttttcgtat cggaaatatt ggcgagatct atccggaaga tattttggaa
    54541 ctgacagcaa ttatcaaaga ctataaggag aacgttaatg attaaaactg taatttttga
    54601 ttgggcagga acaactgtgg actttggctg tatggctcct gtccacgctt ttagaaatgc
    54661 ttttctagaa aaaggaatcc aactgactga caaagaaatc cgctaaccga tgggaaaatt
    54721 aaaatgggat catatccaac agcttctttc cctcccttct gtcaaaaaac aatggatagc
    54781 tatttatgga caactacctc aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca
    54841 ttatctgttt aaagatttgg tcaagcattc taccttgaaa cccgacacat tggaaacgat
    54901 cgaacggttg agaaacgctg ggatcaatat tggttccacg aatggattta atgctcctat
    54961 gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat tcaccagact tcatggcaac
    55021 ttttgaagac gtcgaagggt atggtcgacc gtttccttat atgatccata aaaacatgca
    55081 gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg tctcagatat
    55141 ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta attgaaggta gttcgctaat
    55201 ggggatttct tatgatgagt accaaaaatt agagatgggg aaaaagatcg tttaaaacag
    55261 tctgttgctc ttagattcca acaagccggc gctgatattt gtgtaaacaa tttaaaagag
    55321 ttaacagaat acattttgta cgctgacatg aatccagaat gacttttata caaaataaga
    55381 ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag ccaagcaaaa atagctcctc
    55441 atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttattc
    55501 gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
    55561 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
    55621 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
    55681 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
    55741 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
    55801 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
    55861 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
    55921 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
    55981 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
    56041 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
    56101 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
    56161 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
    56221 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
    56281 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
    56341 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
    56401 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
    56461 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
    56521 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
    56581 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
    56641 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
    56701 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
    56761 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
    56821 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
    56881 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
    56941 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
    57001 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
    57061 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
    57121 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
    57181 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
    57241 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
    57301 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
    57361 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
    57421 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
    57481 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
    57541 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
    57601 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
    57661 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
    57721 ctagtcgttt cataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat
    57781 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
    57841 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
    57901 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
    57961 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
    58021 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
    58081 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
    58141 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
    58201 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
    58261 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
    58321 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
    58381 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
    58441 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
    58501 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
    58561 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
    58621 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
    58681 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
    58741 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
    58801 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
    58861 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
    58921 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
    58981 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
    59041 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    59101 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    59161 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    59221 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    59281 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    59341 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    59401 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    59461 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    59521 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    59581 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    59641 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    59701 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    59761 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    59821 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    59881 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    59941 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    60001 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    60061 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    60121 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    60181 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    60241 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    60301 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    60361 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    60421 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    60481 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    60541 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    60601 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    60661 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    60721 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    60781 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    60841 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    60901 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    60961 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    61021 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    61081 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    61141 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    61201 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    61261 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    61321 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    61381 ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa
    61441 attaaaaaaa taaattatat tatcgttgtt agcgtttact atatgtatta ttgttgtttt
    61501 ttatacacaa atcattcgct tcaaaacaaa ttattcaata ccattgtata ttttttacat
    61561 aataacaaat tatatttgta aagtattaaa acttgtttct aatgttggta tgttaaaact
    61621 ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa caataaggag aggacttttt
    61681 aatgcgtgtt aataaaatcg attttcactt ttcaaaactc agatatcaac ctgattttga
    61741 acatttctcg gatgaagagt tccaacagat caaggaacat actgttcttc gttcttataa
    61801 aaaaggacag atccttttcg acgatggtga tcccagacaa cggttgtata tcttgaccaa
    61861 aggactcgtt cgtttagaac gttatgatga aagtgcaact tattactact atgactatgt
    61921 gaatacaaat aacctttttc ctatgtcagg aatgtttgga gaagagacct acacttatac
    61981 agctcaagcg atgactgata ttgaaacatt ctaccttcct gtttcctact atgaaaaatt
    62041 agcacgaaaa aatcccgaac agttgatcta tatgatacaa aaaatttcta aagtagttga
    62101 aatacatgaa ttgcgtatcc aaatcggatt gacctctagc gcctttgacc gggtaaaaca
    62161 aaatttattc attttgaaag aagaattggg cgtaccaaca aaagatggtc atatcaccat
    62221 tccttatccg atcaccttaa aagaattagc agtgaatagc ggcacgacac gtgaaactgc
    62281 tggacaagta atcaaacaac tacgtgattc cgggctgtta gattatgaca aaaagcgttt
    62341 tactttctgt aagacagcta ctactccagc tatgagagaa taacgcgaac aaaaataagc
    62401 agtgaccctg ataaatatca gggtcactgc ttattttcct ccctaaaact tctgaaagat
    62461 ccggcagttt tagatcactg gacaagactc aaagattttc tatgtgtgtt tcagttatgt
    62521 aatattcact gtttgcaaaa aatactgtta gattaactag atgaagcgtc ttgtcctcat
    62581 ccgatctaga ttattcgata ttcctcgaca ataaacgatt actcagtgag ggcaatcaga
    62641 ggaacggata atgataaata agctgtaaaa aatagcaaaa taacaataat gattaaaagt
    62701 atgtaaaaaa tagggctttg acaaaaacgt gtcacagccc tatttcgatt aaacggtgtg
    62761 tacctgtcaa ctcttcggtc acaagtcata atgttcataa acatgaggag ctgttttttg
    62821 aaattgttcc ttgtgaatca gagttaaacg acaggcttac gaactcttga ttaaacggcg
    62881 ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc aaaaacatga gaagccgttt
    62941 ttcctgggct gttcttatta cttgatgcag ggcagctttt tcacaacctc tttattcaga
    63001 taaaaccaag cttggatcag cattcaactg atagctagca aaatttcttt tttgcatttc
    63061 cacatgtgct gctgctccaa tcattgcagc attatctcca caaagtcgaa gagggggaat
    63121 cactagttct acctcaggga gtttagctgc caaagcatcc tgcaatcctt cacgcaaccc
    63181 ttgattggcc gctacacctc cagctataat cagttgcttg actggataat tttgacaagc
    63241 tcgaagtgtt ttagtaatca atacatcgat cacacttgct tgaaagctag ctgctaaatc
    63301 atttttgtct agatcttctc cacgctgttg tgcattatgc accaaattga taaatgcact
    63361 ttttaatccg ctaaaactga aatcataatt atcttcatgg atcatcgctc gcggaaaatg
    63421 gtaattatct tttccctgat gagctagctg atcgatttct tttccgcttg ggtaagaaag
    63481 acctaatact cttccaactt tgtcataagc ttctcctgct gcgtcatctc ttgtctcccc
    63541 aatgatttca tatgatccat cttcttgcat atagactaat tctgtatgtc ctccgctaac
    63601 aagaagtgcc atcaatggaa actggaatgg tttaaccaga cgggctgcat agatatgccc
    63661 tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc caagcaaaag ccttagctgc
    63721 agaaatacca atcagcaacg agccgacaag tcctggtcca taagtgacag cgactgccga
    63781 taaatcttct gccgacacac cagcttctac cagtgcatct tccaagcata aggtgatttg
    63841 ttctacgtga tggcggctag ctacttcagg tactacgcca ccaaaacgtt tatgactgtt
    63901 tatttgagaa gcaacgatgt tagataagat ctctgttcca tttctaacga ccgctacact
    63961 cgtttcatca caacttgatt caatcgccag aatcaattct tcattcattt catcgtctcc
    64021 gtttttaact ttctgctcat aatgatcgca tcttccgtcg gattgcgata atatgatttc
    64081 cgtttaccta atatacgaaa cttttctgat tcataaagtt ttcttgctgc ttcattagat
    64141 agacgtactt ctaaaaaaac atgcagcgaa tcttttattt gttccgtaat cagtaattgt
    64201 aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg caacagcaat atttgtgatc
    64261 tcgacttcat ctattacttt cgagtatcca ataaagccac agatctggtt atcttcagct
    64321 aaaagcagat aatcagagtg aggctgttgg atgtctgcca aaaactgcat tttcgtccaa
    64381 ggtgaaccat gtggataagc attttcgcta atcatccata cgcattctgc caagtacgct
    64441 tcatcaaaat ctttttttct gcaaatcaac taatctcact cattttctaa aagttttatc
    64501 atatccaacg catcttcttt tgtttcagtg taatatcttt ttttgatcgt tgttgcttga
    64561 aaccccaatt tccgataaag tctttgtgca tcttgattac tcatacgcac ttctaatgac
    64621 atcgtctcac agcgattcat tatagcaaag ttctcgattt cgtcaataag cagcgaaccg
    64681 attcgtttac gctgatatgc cggtcgaacg gcaatattcg tgatatggca atctaaaccg
    64741 aacgctcttg aaccgataaa accaatcatt cttccattat caaagatacc tagatataga
    64801 tgaggaagcg gtgacttcaa ttctgatata aaggcacttt ttgtccatgg caattcgcca
    64861 ttatagactt ctcgttcgac atccagcagc tctttgatat cttctgtagt caattctctt
    64921 agtacgtaac gacgatcgat ttgttcgact gttttttctg tataaatcat tttatcttga
    64981 aaaaagcttc tggcatacgc tctaaatttt ttcaacataa ctttcgtctc caggtgtgtg
    65041 cgtctccaac catttttctt ctgcttctac tcgttttaaa tagcgaggaa gaaaagcttg
    65101 tttctctgca actggttcaa ctaaagcacc taattgagcc acagttacac cacttggaat
    65161 atcccatgct ggaactgtat taatcatcgc actaggcaat atttccttta tttcagagaa
    65221 gaatttgtga caatcactcc cgacgaaata aatagcttca ttcagctttt gtagttgtgt
    65281 gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca gtttctaatc tattttcttt
    65341 ccagcggtat gccccagcgt atacattttt tcttcgagca tcaaacatgg gcacaataat
    65401 attatccaca ccgatgcaat tagccgcgat ggttttcaga ctagatactc ctactagctc
    65461 tttatctaat gtataagcaa tcgtttttgc tgtcgttaca cctatacgaa gccctgtata
    65521 agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct tttggactta tttgaagatc
    65581 tgcaaacaat tggtcgattg caggcatcag tgttacactg tggttgcgtt tgatatttgt
    65641 ttgtatttgt ccaagaattt gttcgtcttc taccacaccg atcgccaaag tttggtttgc
    65701 agtatctatt cctaatgtaa tcatatattt gatcctttct gctatgacat tctcgccttc
    65761 cattgtagca tactttttta ttctgaggat gaaaatacca aaggcaaaac tagtacaata
    65821 atacatgatt atctgctggc gggcaaattt tgccccttcc tgaaacacaa aaaagctgcg
    65881 gtatcactcc attttcccac agcttttcca tcttttattt cgttgattct cgaataatca
    65941 actctgtttc cacaataatc tttcttgaaa ccggtgcttt ggaattactt aattgaagaa
    66001 tcgtctccac tgctagctct cccatccatt cggtataaac ttttacagta gaaagcgagg
    66061 gcgttacata tttcgctaca ctgacatcat tgaagcccat cacagaaata tcttctggta
    66121 ccactaaacc tttttcttga atcgctttca gtgcgccaat cgctaatgca tcattagctg
    66181 caaaaagcgc atcaggaaca tgcggattcg tttttaaata ttgcttcact gcttgatatc
    66241 ctgactcgac agaaaaatct gcttcgataa ttggaccttc gtcgtagcct ttttctgcca
    66301 aaacttgttg aaatgccata attctgggat cttttagccg ctgatggttt ttcttcgtat
    66361 attctgttcc agcaagcata gcgatatgtt gcgctttatg ggagacaaag tgttcaatga
    66421 ctttttgaac acttgactta aagtcgacaa ctagagagtc gattccttga gaagtgccgt
    66481 cagaatctac caaaagcaag gcattttggg atcctttcat ttctaataac tgagcttcgt
    66541 cgaattttcc tagtgcgatc gttccatctg ccggaagatc actaatggaa tcccacgttt
    66601 ctttcaataa agtaacgttt gcttcttctg cctttttttc gattcctagg cgaatagata
    66661 aataataaag atcttcaagc tcttccgtat cgtcgtacca ctggatcagg cgaagcgttt
    66721 gcttagtatt tgctgcattt ttacgatgtt tcgtataatt taagcgttct gctgcttcaa
    66781 aaattttttg ctttgtggct aagccaacgg aaagatcagg atcatagttg agaactcttg
    66841 aaacagtagc aggagaaact cctgccaact gcgcaatgtc tttaattgtt gccatttgtc
    66901 tctcctcctt ttttacgttt ataaaatcgc agtaaatcgt tcaaacgctg cctgtccttt
    66961 ttcatctcgc ttgaatactc cagcgtcttc cagtacacgt gcaaaaacct gacctacaga
    67021 tttttgtacg acatcggtga ccgtatcttt cgtcaaatca ggatgttctt tttttaattg
    67081 ttctgcccac ggacgatgat attcagccat ctggttttct tctcctaata agaaatgctc
    67141 tacttctact agttctggtt tcaaacgagg tggtaaaaca gcaagcccca ttacttcgat
    67201 caacccaatg ttctcctttt tgatatgttg gacatcttta tgtggatgaa aaatcccatc
    67261 aggatgttct tctgaaacat tgttgtctcg aaggacaagg tccaactcaa agtaattgtc
    67321 ccttcttcgt gcaattggcg taatcgtatg gtgtggtgta ccatctgctg aaaaagctct
    67381 gatagatact gtttcatccg aatatgcttt ccatttttcc aatactgagt tagctgcttc
    67441 aataagcaag tttttctctg ttgattgcaa acggatcaca gacatcggcc atttcacgac
    67501 tcctgcgatg acttgcggat aatcttctag tttgataagt ttttctatcg gtgcttttgc
    67561 cataggaaat tcatgacgtc ctgcctgata atgatcatga gaaagaatcg atcctccaac
    67621 aattggtaag tcagcattcg aaccaacaaa gtaatgaggc aagacttcag taatccgaag
    67681 cagtctagca aatgtttcct ttgtgatttt catcgggcgg tgttgttctg ataaaatgat
    67741 cgaatgttca ttgtaataag cataagggga atattggaat ccccagcttt caccgtctag
    67801 attcattcga ataatccggt gattggtgcg ggctggatag ttcaagcgac ctctatatcc
    67861 ttcgttttcc atgcaaagca tgcaggtagg atagtctact tttaccgcct cgcgttctgc
    67921 tgcgatttgt tttggatctt tttctggctt agataaattg attgtgattt ccagttcacc
    67981 atactccgac tctgccggaa atttgatgtt ttttgcgatg gctcttgttt tgatgtagtc
    68041 atttttctta cttaaattaa agaaatactc agttgcttcc tctgggcttt tcgaatagtg
    68101 ctgtgcaaag aatgcattga cgactgaagg tggcggtgtg agaaaatcca ttaattgcgc
    68161 ttcaaaaatc tcttgttctg ctggcaattc tgaaagaaca ccattttctc ttgccttagc
    68221 gacaagctga tccaataagt ctacagattc ttccttaact ggtcttactt caatgctttc
    68281 tagagcatct tctcctacca tccctaaaag acgattctgt aaatataagc ggtccatttc
    68341 catccatccg ccagcttcga tcgcaagtgt ggcaaaatca caaatcgttt gactgattga
    68401 catatctcat tcccctattc ttcataacca tttggatggc tgacatgcca atcccatgct
    68461 gttttgataa tcgctttgat atctgtgtat tcaggtttcc agcccagaat ctctctcgct
    68521 ttttcacttg aagcgactaa tcggctagga tctccagctc ttcttggtgc tatttctgct
    68581 ggaatttctt ttcctgtcac ttctctagct gcttctagca tttccttgac tgagtatcct
    68641 ttgctgctgc ctaaattgaa gaagttgctt tcatttcctt ctttcaagta ttccaaagct
    68701 aaaatatgtg cagcaatcaa atcttctact tgaacataat ctctgataca tgttccatct
    68761 ggtgtatcat aatcgtctcc ataaacagcc aaggcttttc tttgaccaag agctacttgt
    68821 aaaatgattg ggacaagatg cgtttctggt gtatgatctt ctccgataga tgcatctgct
    68881 tttgctccgg ctacattgaa gtagcgcaaa gcgacatagc gcatgccata tgcttggtca
    68941 caccacttca tcattttttc catcatcaat ttactttcac catacggatt tttaggatta
    69001 gctggtgtat cttctgtgat tggagactct ttcggctctc cataagttgc tgcagtagaa
    69061 gagaatacga ttttattcac gttgaactcg tgcatgactt caagtaagat ttgcataccg
    69121 tatacgttgt tgttgaaata catcaatggt ttttcgacag attctcctac taaagagctt
    69181 gcagcaaaat ggattactcc ttcaatcggt tccttttcaa atacggaacg tagaaattct
    69241 ttatcccgga tgtctccttc ataaaaatga gcatctggat gtactgcttg tttgtgacct
    69301 gtgagcaaat tatctaccac agctacttga tacccctttt gaactagctg gtctacagcg
    69361 tgcgaaccga tataaccggc tccgcctagt actaaaattg tcataaaaaa cactcctttg
    69421 attagtaaaa ttgtgtttgt tgattctata taatttaaaa aacgaagttt acttttatgg
    69481 tgaaaacaaa caaagtaaaa tttcgtaaat tcatatggta aatttataat aaaacattta
    69541 gtaaacattt tcaagggttt tttgcaaagg tttacaaaca aacgtaaaac tagccttttt
    69601 ttctcttttg ttccataata gatataacaa ataataaatg aggtggattc aatgaaaaaa
    69661 tttgtatctg gaatattagt aggaagtctg gcaacagccg ctgccgttgc tggtttggtt
    69721 gcttctgtga aaaaaacagt gatcgatcca attgacgaaa aagaagcaat gatcgaagaa
    69781 aaccgtaaaa aagcaatgcg taaacgcgtt tctcgttaat aattatttaa aaatacgcat
    69841 gaagactttc taaaaaacag ataagtcatc atgcgtattt ttatttgtca gtcaatataa
    69901 gttgtgctat tttatctgcc tgtctcagat tccatggatc tgtataaggt ctagctactg
    69961 ctgcagttag ccaatccaca ccttcaacag gtattccata gcaataaaag tcagactgga
    70021 tttcccctga agcatcaatc acgcgattcg tacctcgctc aacatctata gcttctgaat
    70081 gataacttcc tgtattatct gaaaatgaaa aagggcgaac aagtttgttt tccttcaaat
    70141 tttttatgac aggattactt gaacgagaaa agcttgtttg tggcaatcga gcttcaatca
    70201 aatagcgact gctgatttct ttatcttgta gagaaataac atagcggttg tgcttttgct
    70261 gaatatcaaa tggcggttcg atcaaatgaa aaatacccgc atctatcagt gctgctaatt
    70321 ctttcgtccg aatgattggc ggaccgatcg ttaaaaagct atttagatga gtaaaccatc
    70381 cccatagtat ttctttgtat tccttagcag aaaaaacgtg ccattcaata gcctgccgga
    70441 ttggatcacg ccagtctttt aatgcatcaa atgttgaagc aaatgctcct gtgcaattgc
    70501 ctttttccgc ttcttttatc tgcccttcaa taaatattcg gctagcttct gaaaagggct
    70561 cattctgata ggcaagagga gtttctaaat acgaccaatc ccatctataa gggattagtg
    70621 ctggatgctt tccgagacac tcctctgaag agagggagag aaaatcatgc tcaaaatttt
    70681 tctttggaat cggcaagtga tattcttcga tgattttctt gtaataaaac aactcaacgt
    70741 cttttttcag atagtcaaaa aatgtttcgc cagtcaaaaa tcccgactga tgccattgct
    70801 ccaggttttc ttttgttagt aaccgtggcc atgccattgc tccgccttgc ttttgatttc
    70861 ttcctcgagg gtaataaggg agcccccttc ttgatccact gtatacaatc ggttccttgc
    70921 ctgatggatg atagaccagt tttccctttt cgttctcgaa gcgccctcct cttgatactg
    70981 taaacaatcc gacataatca aaaaaactta agcctagacc tcttaaaaag acattttctt
    71041 taggcggtat cttcgaaaga tctacatctg caggattaga aggaagctga tacagtaaaa
    71101 catttttctt tgcgtagtct acccacactc tttcttcttc tgtctcatca ttttcccagt
    71161 gaccgcttgc taaaacaagc tgatcgatag aatagctttg ctgatctgtc tgtaaaagat
    71221 aaccatcttt tcttttgctt acttttttta ctaaagaata aacaaactga atagaagctg
    71281 gaaattcttc ttgcaacttt gagaaaaacc agcgttgata gagtccgtat aaacatcgag
    71341 tagattgctg atctttttcc agacgattga tttcttctat aattagttgc ttatttttgg
    71401 ttaccgtctg ttcgatataa tctgtagcag gtcctttact ccattgaaac aagttgggtc
    71461 cctgtgttac caatcctcca ctggacaatg tttcatctgt aaaaagcgtc acttgttgag
    71521 aaacagaatt cattaataac tccggtggct gattcaaccg ccaaactcga cctccaggac
    71581 cattgggatc gaaaagaaaa agttcaagag aatgtctgtc ctgattatta tggagaatcc
    71641 tttctgctac acttaatcct ctaggtccgg caccaatgat ccctattttc atttgcatgc
    71701 ctccatttcg ataaattttt tcaataaaac agcatggttc tgatgttcat tttttgcacc
    71761 ataaacaaaa gtaatgactg gatgttcttt cataagttga ctgagtttaa aaatagcttg
    71821 cttcttttct tcttcgaaaa tcaattcttg ctggtatttc tttgcaaatg aagcaaatcg
    71881 ttctggtaca tggtcaaaag ttttgcgtgc ttcattacta ggtgcaattt ctttcaacca
    71941 cagatctaac tgcgcttttt cttttgaaat tcctcgtggc cagaatctat ccacgagtat
    72001 tctatagcca tcctcttttt cagcatcaag gtaaacacgt tttaatttga tcatagttgt
    72061 ttccttcttt ctaccttttc gcttagctta tatatgtaga taatagcaca aaataaaaaa
    72121 aatgaacggc acaactatcc attgcgccgt tcatgtgatc ttggtcaaaa tctaatgatt
    72181 gattcggttt ttaaagctgt catacaatca tctgttttat acttttttac ttttgatttt
    72241 tccagaccat ccgtcatatc cacccttaag taaataaatc tcttgataac ctgctttacg
    72301 taatttattt gctgtacgga tgctcaaact tttcttttga tcgtaaatat agacaggctg
    72361 gtcttttcga atagatccta atgaatcttt caaaacagtg taagggatgt ttcttgcacc
    72421 taaaatatgc ccagcatcaa acacatcttt ttcgcgaaca tcgattactt gtgcttttct
    72481 cattccttct cgaaattctt cttctgtcaa agtcgttgct gaacgtttgg ccattactcg
    72541 tagatagact tcccaaagga tgattgcaag cacaatcaat agcaaaatga tattaattac
    72601 ccataaaacc atgtaatgcc tccgtttttt taagcaaatt tcaaggctaa tgaggcagca
    72661 ccaatgacac cagcttcatt accaagctct gctaatttga tttttgttga ttctcttact
    72721 tgcgggaaag taaattcttt aaaatatttc tctacacggc tacgtaaaaa ttctcctgct
    72781 gccgatactc ctccacctaa cacgatactt gacggattta atgtattccc caagtttccg
    72841 caagctaaac ctaagtagaa gcatacgcga tcaacaacca tcaatgcaaa cggatcgtct
    72901 tcttgagcca tttcaaagat atctttactt gttacttctt ctccgttgtc tagcattgct
    72961 ttcagttttg aatcccctgc gtattcttct gccaagaaac gacctaggcg taccacacct
    73021 gtagcgctag atacagtttc taaacatccg cgtttaccgc aagtacattc gaacccttca
    73081 ggatccacag tgatatgtcc gatttctcct ccagctccgc tcacgccgtg aatcaaattt
    73141 ccgccagcaa cgatacctcc gccaacacct gttcctagag taacgaatac aacgtctgga
    73201 tcgttttctc cagcaccttt ccatcgttca cctaaagcag caacattcgc gtcattatcg
    73261 atagcgaatt tgattcctgt tcctgcctcg atcagttttt tcacaaattg aacttctgtc
    73321 cagtttaagt tatacgcacc aataaccgtt ccagcttcgc tgtccacgct tcctggggta
    73381 cccatgccga ttccgataaa gtcttcggct ttcatattat ataaattcaa acgatgattg
    73441 attgattcaa tgatttcagg aacgatatgc ttaccatcat ccaaaatatt ggtatcgatg
    73501 ctccattttt gctgaatctc tccttctggt gttaaaattg caaattttgc tgttgttcca
    73561 cctaaatcaa tcccaataat cttcttgtcc attacaaatt tcctttcttt cggctttctt
    73621 caattcggtg ctctcttttt aatatgcgcc acgctgtcat gtatgtatca tgatcaatca
    73681 aacggctatc atacagatta cgcaattcga tagtcattaa ttcaatgtca tagatgcgtg
    73741 cacccacata gataaagatg ccaaattttt tgaacagctg ctgtacatca tataagctct
    73801 ccatctttaa gcacttcctt catattatac aagtaaacca tattttttta aacccagtaa
    73861 aatacatatt acaaaaagaa atacaaaaac tagtgtagaa agaatccttt ggtgtatcga
    73921 atagcttgtc tgttgtttcg gtagacccaa aacattccct aacactaatc cgccaattag
    73981 cccaccgaca tgtccccaaa catctacaga ggtatccaga agaccaaaaa caaatgtcaa
    74041 cacaataaat aaaagaaaat gtctgactag ctgctggatc gctgggttat gtttaaaatg
    74101 aaaccctaag ataaacaacg caccgaacaa accgaatatc gaagtacttg caccaccaga
    74161 cagaacattc gcttcgttaa aagcaaagct tgctgtattt cccattatcc cgctgaacat
    74221 ataaatcaag aaaaaacgcc aatggccata gattgcctcg atttgctgcc ccataaaata
    74281 cagtatcagt gagttcatta cgaaatgcat cagtccaaaa tggatgaaga ttggtgtcac
    74341 taaacgccac cattcattga agtgaacaag ataaggacca tacattccgg cataataagg
    74401 gatctcaagt cgcggaaata attccatcat gaggaagacc gctgtctgta tccctaaaaa
    74461 agcataggtc cataacggtc gtcttaacca tgcacgcaat tttatttgct gttgataatt
    74521 catgaaaacc tcccatttca ttttagtcaa taaacaattg ttggatcttc tgatcaaagc
    74581 tctccggttg ccattgttca ttcaattgtt cacgaaaaac aaggctacat gtttttttat
    74641 gaaaattatt taaataccgg tcgtaataac caccgccaaa accgattcga taaccggctc
    74701 tattgtagat aatacctggt acaatgagga gatcgatcat ctccggcaaa tagagcttct
    74761 ctgattgtgg ctcaagaaca ccaaaactcg aacgtacaag aacggactgt tcatccattt
    74821 cataaaacgc catctgacgt ttcggcaaag ttttaggtac aactattttc ttttgttctt
    74881 tttctgctcg ttggataacc ggctgtgtat ccagttcaaa ttcattcgat aaagtcgtcc
    74941 ctatcacttt tgctgacttc cagtactcgc tggcgaatag ctgtttatag attcgctgtt
    75001 ctttttcctt tttcttgcca cttttggcca gatttttcag cttttcgatt cctttccggc
    75061 gtaatcttac tttcaattgg tccaaaaact cacccttcct tctctcataa aatggtatag
    75121 gaaaaaaaga aaaatagcaa tcgaactttc aaataaatgc ataaatagaa taaaaagaag
    75181 acctagaaat gattggcctt ccttttttaa tgaatttcaa ctgttcagcg cgtgtttttg
    75241 tctgataaaa agtgcataca gaaaatatgg cgcaccaaaa agggcaatca aaattcctgt
    75301 aggtacttcc ccacttggta gaatcactct agcaaccata tctgcactaa gcagaaaaat
    75361 ggaaccgaac aaaccgctca gaacaaaggt ccagttattt tctttacgta cgactagctt
    75421 cgcagcatga ggagctagta gtcctacaaa agataaacta cctgtcaccg ctacactacc
    75481 gcttgccaaa gcaacagcta acaacaaaag gattttttga cttttagcca cgttaaggcc
    75541 taatccttgc gcgcgttctt gaccaaatga tagcaataca atcctctgtg cataaagtcc
    75601 tcctagcgga attaagataa ccgcccatgg aagcaatgcc caaacataag gccagctcgc
    75661 accccagatt gatcccgcta accatgcatt cacaaatcca taattttcct tagataattg
    75721 gatcgtgcat agcaatgtta atgcagaaag gccggcatta acggcaatac cggacaataa
    75781 gattcgattc atacccaaaa agccatttcg atcataggcc gcaagataga cacagcaagc
    75841 agctaaaaca ccacctagaa aggctacaac tagcaataga aacggcagcg aatagtgcga
    75901 aaagaaacct aaataaagca tcactaaaaa tcctgctccg gcattaatgc ccaatatact
    75961 agaatctgcc aattcatttc ttgttactcc ttgcaagata taaccagaaa cgccaagaca
    76021 agctcctgct aataaactga tcagtaatcg aggaactcga aattctctaa caatcagcgc
    76081 caaagaagat gattctttcc ctatgaacgt ggcaaaaagc tgatccaggt aaataaaagg
    76141 cgtaccaatc attaaactac ctatagacag gatgacaagt acaaaaagca agaaatagaa
    76201 cgtccaatat atttttttca ccgttgttcc ctccttactt gtatcagtaa aaacggaacg
    76261 ccaatcaatg caatgactaa tccaaaaggt gtttcaaaag ggggattgat catcctcgct
    76321 gtaaaatccg ccaacataac caatccaccg ccaactaaaa aagagacagg tagaacataa
    76381 cgatagtccg ttccaaccat tccgcgaaca actggcggaa ccaacagccc aataaatgaa
    76441 acaggtccga ctaacgaaac ggcaatggct gctaacactg caactagaca gaaaacaatc
    76501 aaccgcgttc gtttaggatg acttccaaga gctaatgcat gtgtttcact caaatgaatc
    76561 aaagatattg cttttcctaa tgaaaaagaa accaaaacag caagtatata taaaggaaac
    76621 aatactgcta attgtttcca tgtgatattg gcagctccgc ctacaaacca aaaagcaaga
    76681 tcctgctgca gattaaagaa ttgggtaagt gcttggctga tagcagtaaa aaaagaacta
    76741 atagctacgc cagctaaaat cagctgtaaa ggattcatgc ccattttcga aaactgcaaa
    76801 aaaccaaata cgagcaaagt tacagcaaaa gaacctgcca atgacattag aaacacagaa
    76861 aaatcagaag ctttaggcca aaaagcaaaa ctaagggcca ttgccaacga agcacccgcg
    76921 ttgattccta gtaagcctga atctgctaaa ggatttcttg tcaccccttg catcaatgca
    76981 ccgctcaatg caaaacaact ccctacaaga aatgctcccg ccacacgagg caaacgaata
    77041 tttcgtatga tttggtgact ttgttttgat ctatcaaaat caaacaacgc ctgccaaaaa
    77101 tctgcttttt ccacagtcgc tgcgccattt gaaagagcaa caaaaaaaat aatcaaaaga
    77161 acaaaacaga ccaaaagcag gagtctattt ttcttcatag tcctcataat tgatcctcct
    77221 gctcttttgg caacaaatca taggttagaa aaactggttt atttgttctt ggaagcgtga
    77281 caatcgttgc atcaatctca aatatttttt tcatacactc ttttgtgatc acttcttcag
    77341 gtgtaccttc tgtgatcaac ttaccgtttt tcattgctaa taaataatct gaaaaacgtg
    77401 aagcatgatt aatgtcatga atcgacatca agatcgttgt ttgtttcatt tgattgattt
    77461 cagtcaataa ctgtaggatc tctaattggt gtgccggatc taaaaatgtt gtcggttcat
    77521 ccaagatcaa gatatctgtt tcctgagcaa gcgccatagc gatccagaca cgctgggttt
    77581 gtccaccaga cagcgttgcg atatcccgca tacgaaatcc agctagacct gtagcatcca
    77641 acgcccactc aattgcttca tgatcttgcg tgtccatgcc actgaacagt ttttgataag
    77701 ggtatcgtcc gtaagatact acttcttcta ctgtcatacc tgtagggtgt tcactagttt
    77761 gtgctaataa tgcaattttt tttgcaagtt cttttgaact gtatgtagag atatcagagt
    77821 tattgacaat gatctttcct gatttcacag gtagtatacg ggtaaaactt ttcaataaag
    77881 tagattttcc acttccattt ggcccaatca aactagtgat tttcccttca ggaatcgtaa
    77941 cagacatgtt ttcaatgatt tttttgtttt cgtagccggc tgcaatgtca gctgctctta
    78001 atctcgtcat ttcacccatc ctttcgctga aaatcattct caattatgac gctgtttcaa
    78061 catttcgtca agcgctactt tcatatctat agccaaatga agcggttgac agcgttcgaa
    78121 atatcaggaa taataaccaa tgagaaagat tctcaattat gttaggagga aagaaatgaa
    78181 attaaaaaca aaagtattca cattatctag catattgctg ctggctggag tactgagcgc
    78241 ttgctccccc tcttcagata aagaaacagc tacaactgcc aattcgacga cttcatccaa
    78301 tagtactcat acagtcacag atacacttgg acataaagta gagattccaa acgcgcccaa
    78361 acggatcatc ggcagttact tagaagacta tctgattgca ctaggggaaa aacctatcgc
    78421 ccagtggact gttggcagtg gttccattca agattatctg caagatgatt taaaggatgt
    78481 cccaacgatt tcttatgacc ttccttatga gaaagtgtta agctttgagc ctgatttgct
    78541 gttgatttct tcttccgcta ctgtcgaagg cgggaaatac gaacaatata gcaaaatcgc
    78601 gccaacttat gttgtaaaaa acggaacaga tgtcacatgg gaagaacaac taaaagatgt
    78661 cggcaaagct ttggcaaaag aaaaagaggc agaaaaaatc atttccgatt atcaaaaatc
    78721 catcaagaaa actcgagaag aactagcaga taaaatcgag aacaagacag ctgcagtgtt
    78781 gtgggtgacc aacaactcag cctttatggt cagcaaaaat cggtcaagtg gaagaatcgt
    78841 atacgacgat ctggaatttg gtgttcctga tctagtggaa gaggtctcca aaaaagccac
    78901 ttctgactgg tctgctgttt cctctgaaaa gctagctgaa ttagatgctg actacatcat
    78961 ccttgtgaac agtgacaaag atgctgccat gttcaatgaa ccaagctgga agaatctaaa
    79021 agcagtgaca gacaatcatg tactgcaatt cggacctgaa tctagttggc tatataacgg
    79081 acctattgcc agtcaggaaa tggtcaatga tatcaaagaa aaactaaatt gattttaact
    79141 aaaaaaagca tcttcacgat tagtcgtgag atgctttttt tcttattaac aaactagacg
    79201 catagatttt attcttcttt tgaagcaacc ctatttttta ctctttccca tttttgaaaa
    79261 cgatgggcat atagattttc ttgatccgtt atcccctcat actcctctat aagggcgaag
    79321 tctgaaaaat caatattccc aatatacgta tcgccttcaa acgtgctatc gatcaatgtt
    79381 cgccagatcc gaccagtgta aggaagaagt tcttgaaaaa tccgactacc acccgaaaca
    79441 tagatatctt ctgagatcgt ttgactatac gacaaaagtt catcgatcga atgcattact
    79501 tccgcatttt ctttcgtgcg aaaatttggt tgcgttgtca acacgatgat atggcgataa
    79561 ggaagtgaaa gatcccccat tccttcatag gtctttcttc ccatcactaa aattcgatcc
    79621 attgtatgtt cgcgaaagaa acgcatatca tttggcaatc gccatggtaa caaaccattt
    79681 ttgccgatca gaccattttt atcttgtgcc cacatggaaa taaacatatt atccgctcct
    79741 tatgatttta gatgttttga gataaaaaaa gtctcaattc tttgaccagc aaggaattga
    79801 gaactttaaa aacattattt tgtttcacgg tgcaaagtaa ctttgcgttc gcgtgggcaa
    79861 tatttttgtt tttccaaacg atcaggattg ttacgtttgt ttttgcttgt tagatagtta
    79921 cgttctttac aagaagtaca ctctaaagtg atgtttacgc gcatgttttt ccctcccatt
    79981 tttacaagaa tcctctaaga tgactctcag acttgattat cttaccatga ttttttctag
    80041 attgctagtg tttttcacga ttttgttaag taaaactact tgacgataaa tacaacggtt
    80101 ttacttctca aacaaaatct ttttcttttt gtataaatac ccttacactt gtttatatag
    80161 attatgtttt ttaataaagg gggatactaa tatgccatgg aacatgaagg actttcctgc
    80221 atcaatgaaa aacttagata aattgacaag aaaaaaagca atcgacatcg caaatgcatt
    80281 attagatgaa gggtaccctg ataaccgtgc gatcccaatt gccattgatc aagcaaaaaa
    80341 atggtatgaa gatgctagtg aatcagaaaa acgaactttc gaaaaagaaa aaaatccaag
    80401 caagacggat gaacacgata cgaatcctcg tgctggaaaa ctgcttgatt cagatgtgat
    80461 cgtagaatat gaggaagaac aatggatcgt gaaatctaaa ggggcaaaaa aggcaagcaa
    80521 ccattttgat acaaaaaaag aagcaatcga aaaaggaaag caagtagcac aaaacaaaga
    80581 atcaactttg ttcatttata aaaaagatgg gacaaaagaa aaagaaatcg attattgaac
    80641 atatgtaaag gggttgtgac gaaaatcgtg tcacaacccc cttatgttcg aaacaatggt
    80701 gttcctctgc caactccgaa ccataagtca taatatctat aaaacgggaa gagctgtttt
    80761 ttgaaattgt ttcttatcac tcagtgttaa acggtaggag aaaacctctt ttttattgat
    80821 atttgatttt catcaaacag tcacatagtc gattgcttct gcttgcggaa tcgtttcatc
    80881 tacaaagtag actttatacc atgtcaagaa actgaagatt gtccagattt ttctttgctc
    80941 ttctttacgt ccttcgtgat ggtcatctag caacttcatg attttttctt gatcaaaaaa
    81001 ttcttttgca aaatctgctt cgaataatgc tttgacttgt tcgtagccgt gttcttcttt
    81061 caaccaagct ttgattggca cagggaatcc taatttcaca cgattcgacc attcttccgg
    81121 aagatgtttg ttcgcagctt gtctgaaaat atcttttgtg ttgttttgat tcaataaaaa
    81181 cttacttggt atttgttgag ccaatttcat gacttcgata tccaataaag gtactcggac
    81241 ttccaaagaa cttgccatag aaagtttatc tgcctttaac aagatatctt ttggcatcca
    81301 ttgatgaaga tcgacatatt gcatcttatt gacttcatct ttgattcctt ctgttttctc
    81361 gtaatgaaca gtcatgattt catccacaga tggtgctgtt tgatattttg gtgttaacac
    81421 ttctgatgct tctgattctt cgaacactcg agcatgaccg ataaaatatt cttttgcagg
    81481 agcaagtgat tcatataaat ggatccgtcc atggaagttt ttcatttttc cgattttacg
    81541 tcctaaatta tagcgtgttc cttttggtaa ttttttcaat ccttgagcaa tcacacggat
    81601 gaattttgaa ttcgtatgga atccatacgt ttggtatccc gcaaacagtt catccgcccc
    81661 ttctccagat tggactaccc gcacactttg tgaagcaaga tttgctaaaa agtacaaagg
    81721 tacgcaagaa ggatttgaat caggttcatc taagtgatac tggatcaaag ggaatgcttt
    81781 gaacgacatg tctccgtcga tcactgctgc tgtattatct aagtccaata gttcggttag
    81841 ttttctggct tcaatcgctt cattataggt tttatcatca aacccgattg agaaagaatg
    81901 atctggtttc aatacagagg ttacataact ggaatcaacc ccgctggata agaaagaacc
    81961 aacttctaca tcactgaccg tatgtgcttc aatagaagct tgaacggttt cgtcgatttt
    82021 ttcgatccat tcttgtcggg aatgcgtttc ttttgtttca aaatctgcat cccaatattg
    82081 ctgaatgtct atttttccat ctttatacgt ataataatgg ccttcaggca aacgatagac
    82141 atctttgaag aatgtttcgc cgtttaatgg agaatattgg aaagtcatat aaggtttcaa
    82201 cgcttctttg ttcaattctt tgttaaaatc aggatgaggc agaaaacttt tgatttctga
    82261 accaaacata aatgttccgt tcatttctgc ataataatat ggtttgatcc caaagtggtc
    82321 tcttgcaccg aataactcat tcttttcatt atcccaaata gcaaaagcaa acattccgcg
    82381 gatcttttgc aataattctg ttccccattc ttcgtaacca tgcaaaagga cttctgtatc
    82441 tgcatgtgtt tgaaaaacgt ggccagctgc aatcaattct tctctcaaag gctggtaatt
    82501 atagatttcg ccattaaaaa taatgatctt tgtccgatct tcattgtaaa taggctgagt
    82561 accgccctct aagtcaataa tacttaatct tctgaatcct aaagcaacat gtttatcgat
    82621 atattctcct gaactattag gtccacggtg aacgattcga tccatcattg tattaataat
    82681 tgtttttttg ttgtccttgt cgttaacaaa acctacaata ccgcacattt aactttcaca
    82741 tcctaatata aaattactgt ttatttcatt catatgcatc ttatcataga ataaatagcg
    82801 gacgaatatt ttctctctta caatttcgtc acgtgaaaac aaaagatgaa agtttctgtt
    82861 tttacagaaa ataaagtccc aatatctaat ttaccatgtt ttcttttttg aggctacctt
    82921 attctcttcg tgttaaaaaa atattagtgg atgctatttc caaaaaaacc agcaaaaaaa
    82981 gacacgacct aaagatcgtg t
//
LOCUS       DAFCNJ010000007        76746 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000007 DAFCNJ010000000
VERSION     DAFCNJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.007"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="LNE35_000623"
     CDS             complement(105..629)
                     /locus_tag="LNE35_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6909807.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="LNE35_000624"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="LNE35_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBK6909808.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="LNE35_000625"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="LNE35_000625"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBK6909809.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="LNE35_000626"
     CDS             complement(2351..3031)
                     /locus_tag="LNE35_000626"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK6909810.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="LNE35_000627"
     CDS             3157..4026
                     /locus_tag="LNE35_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6909811.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="LNE35_000628"
     CDS             4052..4567
                     /locus_tag="LNE35_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909812.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="LNE35_000629"
     CDS             complement(4614..5207)
                     /locus_tag="LNE35_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6909813.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="LNE35_000630"
     CDS             complement(5232..5411)
                     /locus_tag="LNE35_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909814.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="LNE35_000631"
     CDS             complement(5488..5997)
                     /locus_tag="LNE35_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909815.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="LNE35_000632"
     CDS             complement(6093..6473)
                     /locus_tag="LNE35_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6909816.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="LNE35_000633"
     CDS             6803..7552
                     /locus_tag="LNE35_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBK6909817.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="LNE35_000634"
     CDS             complement(7736..9013)
                     /locus_tag="LNE35_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909818.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="LNE35_000635"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="LNE35_000635"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBK6909819.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="LNE35_000636"
     CDS             complement(9935..10879)
                     /locus_tag="LNE35_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBK6909820.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="LNE35_000637"
     CDS             complement(10881..11567)
                     /locus_tag="LNE35_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBK6909821.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="LNE35_000638"
     CDS             12041..12361
                     /locus_tag="LNE35_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBK6909822.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="LNE35_000639"
     CDS             12515..13420
                     /locus_tag="LNE35_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6909823.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="LNE35_000640"
     CDS             13417..14190
                     /locus_tag="LNE35_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6909824.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="LNE35_000641"
     CDS             complement(14371..15795)
                     /locus_tag="LNE35_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6909825.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="LNE35_000642"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="LNE35_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK6909826.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="LNE35_000643"
     CDS             complement(16517..18499)
                     /locus_tag="LNE35_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6909827.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="LNE35_000644"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="LNE35_000644"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK6909828.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="LNE35_000645"
     CDS             complement(19412..20473)
                     /locus_tag="LNE35_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK6909829.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21250)
                     /locus_tag="LNE35_000646"
     CDS             complement(20684..21250)
                     /locus_tag="LNE35_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6909830.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="LNE35_000647"
     CDS             complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="LNE35_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK6909831.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="LNE35_000648"
     CDS             complement(23038..24159)
                     /locus_tag="LNE35_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK6909832.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="LNE35_000649"
     CDS             24285..25226
                     /locus_tag="LNE35_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK6909833.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="LNE35_000650"
     CDS             complement(25376..26272)
                     /locus_tag="LNE35_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBK6909834.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="LNE35_000651"
     CDS             26541..27881
                     /locus_tag="LNE35_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6909835.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="LNE35_000652"
     CDS             28099..28542
                     /locus_tag="LNE35_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBK6909836.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="LNE35_000653"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="LNE35_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK6909837.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="LNE35_000654"
     CDS             complement(30867..32090)
                     /locus_tag="LNE35_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBK6909838.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="LNE35_000655"
     CDS             complement(32121..32960)
                     /locus_tag="LNE35_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK6909839.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="LNE35_000656"
     CDS             complement(32977..33657)
                     /locus_tag="LNE35_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909840.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="LNE35_000657"
     CDS             complement(33659..34732)
                     /locus_tag="LNE35_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6909841.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34788..35026)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35069..35302)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35379..36050)
                     /locus_tag="LNE35_000658"
     CDS             complement(35379..36050)
                     /locus_tag="LNE35_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6909842.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="LNE35_000659"
     CDS             complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="LNE35_000659"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBK6909843.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="LNE35_000660"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="LNE35_000660"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBK6909844.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="LNE35_000661"
     CDS             complement(39933..40799)
                     /locus_tag="LNE35_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6909845.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="LNE35_000662"
     CDS             complement(40959..43052)
                     /locus_tag="LNE35_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK6909846.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43801..44424)
                     /locus_tag="LNE35_000663"
     CDS             complement(43801..44424)
                     /locus_tag="LNE35_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909847.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44583..45980)
                     /locus_tag="LNE35_000664"
     CDS             complement(44583..45980)
                     /locus_tag="LNE35_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6909848.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(46012..46983)
                     /locus_tag="LNE35_000665"
     CDS             complement(46012..46983)
                     /locus_tag="LNE35_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6909849.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            47357..47782
                     /locus_tag="LNE35_000666"
     CDS             47357..47782
                     /locus_tag="LNE35_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909850.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48270..48971)
                     /locus_tag="LNE35_000667"
     CDS             complement(48270..48971)
                     /locus_tag="LNE35_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK6909851.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48987..49427)
                     /locus_tag="LNE35_000668"
     CDS             complement(48987..49427)
                     /locus_tag="LNE35_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK6909852.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49427..50002)
                     /locus_tag="LNE35_000669"
     CDS             complement(49427..50002)
                     /locus_tag="LNE35_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBK6909853.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(50850..51329)
                     /locus_tag="LNE35_000670"
                     /pseudo
     CDS             complement(50850..51329)
                     /locus_tag="LNE35_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(51488..52189)
                     /locus_tag="LNE35_000671"
     CDS             complement(51488..52189)
                     /locus_tag="LNE35_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6909854.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(52297..53706)
                     /locus_tag="LNE35_000672"
     CDS             complement(52297..53706)
                     /locus_tag="LNE35_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBK6909855.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(53754..55619)
                     /locus_tag="LNE35_000673"
     CDS             complement(53754..55619)
                     /locus_tag="LNE35_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6909856.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(55740..56591)
                     /locus_tag="LNE35_000674"
     CDS             complement(55740..56591)
                     /locus_tag="LNE35_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6909857.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(56901..57614)
                     /locus_tag="LNE35_000675"
     CDS             complement(56901..57614)
                     /locus_tag="LNE35_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK6909858.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(57682..58008)
                     /locus_tag="LNE35_000676"
     CDS             complement(57682..58008)
                     /locus_tag="LNE35_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK6909859.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(58131..58358)
                     /locus_tag="LNE35_000677"
     CDS             complement(58131..58358)
                     /locus_tag="LNE35_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK6909860.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(58680..59933)
                     /locus_tag="LNE35_000678"
     CDS             complement(58680..59933)
                     /locus_tag="LNE35_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6909861.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            59994..60734
                     /locus_tag="LNE35_000679"
     CDS             59994..60734
                     /locus_tag="LNE35_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6909862.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(60820..61578)
                     /locus_tag="LNE35_000680"
     CDS             complement(60820..61578)
                     /locus_tag="LNE35_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK6909863.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(61575..62603)
                     /locus_tag="LNE35_000681"
                     /pseudo
     CDS             complement(61575..62603)
                     /locus_tag="LNE35_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(62623..65790)
                     /locus_tag="LNE35_000682"
     CDS             complement(62623..65790)
                     /locus_tag="LNE35_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909864.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(66014..66898)
                     /locus_tag="LNE35_000683"
     CDS             complement(66014..66898)
                     /locus_tag="LNE35_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK6909865.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(66993..67430)
                     /locus_tag="LNE35_000684"
     CDS             complement(66993..67430)
                     /locus_tag="LNE35_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909866.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(67647..68558)
                     /locus_tag="LNE35_000685"
     CDS             complement(67647..68558)
                     /locus_tag="LNE35_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK6909867.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(69015..69443)
                     /locus_tag="LNE35_000686"
     CDS             complement(69015..69443)
                     /locus_tag="LNE35_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909868.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(69471..70109)
                     /locus_tag="LNE35_000687"
     CDS             complement(69471..70109)
                     /locus_tag="LNE35_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909869.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(70113..72095)
                     /locus_tag="LNE35_000688"
     CDS             complement(70113..72095)
                     /locus_tag="LNE35_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBK6909870.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            72275..72940
                     /locus_tag="LNE35_000689"
     CDS             72275..72940
                     /locus_tag="LNE35_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909871.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(74875..>76746)
                     /locus_tag="LNE35_000690"
     CDS             complement(74875..>76746)
                     /locus_tag="LNE35_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBK6909872.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT        22264 a        15944 c        13070 g        25468 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    43501 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    43561 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    43621 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    43681 acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    43741 attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt
    43801 tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg
    43861 attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt
    43921 cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca
    43981 ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac
    44041 ttttccattt ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa
    44101 atgcttgtct ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt
    44161 aataaattga ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta
    44221 attggcagtt attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc
    44281 tactgattct ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat
    44341 ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag
    44401 aagaaacggt ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta
    44461 ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa
    44521 cttctttttc acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt
    44581 tcttaagcct caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg
    44641 attgttcttt ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat
    44701 gcattggtcc acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca
    44761 ccttcttgaa tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa
    44821 tcatcgtgga tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg
    44881 ttttcagaaa taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca
    44941 tagattcctt gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt
    45001 tcataattgt cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct
    45061 tttgctttta cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct
    45121 ttgataaccg tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc
    45181 tcaatcaatt cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag
    45241 aacgcatctg ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag
    45301 cttggtgtca gattcaagat gataccaata acaccacctt gctccatttc atggtaagat
    45361 ttgatggcta acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata
    45421 tctgactctt ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt
    45481 tcattatgag taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca
    45541 tatttttcat aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac
    45601 atcggcatat cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca
    45661 tcaaaataac gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt
    45721 atcaaacggc tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac
    45781 tgtacatcct cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca
    45841 aagaactttt gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg
    45901 cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct
    45961 ggaaattgat attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat
    46021 tccatgtatg atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat
    46081 tcgttttaac agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt
    46141 gattttcgga aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat
    46201 atccttcccg attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt
    46261 gtaattgctg acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata
    46321 gccgcttttt ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg
    46381 gaccgcttct ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc
    46441 aataaatccg atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact
    46501 ttctcgatca ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc
    46561 gataactgga atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa
    46621 gaagacaatc agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt
    46681 ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct
    46741 ttcgatccct gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc
    46801 aatagagaag cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa
    46861 cgctgaaatc gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa
    46921 gtaacgggaa actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc
    46981 catacattct cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat
    47041 gaccgcattc tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt
    47101 agagcaactt cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt
    47161 aatatagcat ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg
    47221 attttttgcc tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg
    47281 tttttctctt ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc
    47341 aaatggggga agtattttga agaaattaat cgtactattt attttgtctg cgggactttt
    47401 gacattgact ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga
    47461 ttcttctgtc ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt
    47521 acgtaaagat atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg
    47581 gacacgcacg tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt
    47641 gctgacagat atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa
    47701 taaaaaagaa acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga
    47761 tggcaacatc tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt
    47821 ttctgctata gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc
    47881 ctgattattg tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa
    47941 gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat
    48001 cttatcctgt tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc
    48061 actatggctg atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag
    48121 taaaacaaac agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca
    48181 aatatttgaa tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt
    48241 tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg
    48301 ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc
    48361 tgacggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc
    48421 acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac
    48481 gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt
    48541 tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc
    48601 cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac
    48661 tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta
    48721 tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct
    48781 aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa
    48841 taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc
    48901 tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa
    48961 atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc
    49021 aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt
    49081 tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc
    49141 aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag
    49201 tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact
    49261 tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag
    49321 tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg
    49381 atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct
    49441 tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat
    49501 tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag
    49561 tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct
    49621 tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac
    49681 ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt
    49741 catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa
    49801 aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa
    49861 tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca
    49921 tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac
    49981 cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc
    50041 tacacgtgtt tttttaaaat aaaagactag attttgtagt tttttagtgg tatataggat
    50101 agacgccact atatatagta tttagaaaaa aatcattttt cagccatttt atagataaaa
    50161 caaaaataaa taacgtgttt tcatcgatat gaaatgactt acttatagaa gatactggga
    50221 tttcgtccta tatgccaata acgtgtatta ttttaaacaa caaatacttg tttcttttat
    50281 taacgaaaaa atcattttgt tctcgtgtta tgtttcactg ataaatgatg tttctctaaa
    50341 taaaaaagcc gacgaactaa acgatcaaaa tttgatcatt tttgttcatc agcttctcct
    50401 aaaaatcttt ttgttaacga taaatagctg gatttgtaaa aatatcactt gaatcgtcac
    50461 tgctggaaga aaattcagtg ataaccgcac ctgttgcgcc tgcctggaac tagtgtttga
    50521 tattgggctc gaacgtgtac tgatctctag catgaagaat aatttctttt tttactgtat
    50581 aataactttc tttccttttt ggaatagtac aggaaagttt ttttgtttcc tttccttcga
    50641 cataaggtat accagaccct ttcgacaacg aaaggttctc atttttcctt catcgtcatt
    50701 tcaaggaggg tgccgatgct ctggacagtt tcgatcgctc ctaaaccaaa atctgtatat
    50761 tcgattcttt ctaactctga ttctttcata ccattcctgt ctcaaggtac atctattgta
    50821 cttttttctt gtaatccatt ctgcctctcc taactaattg tcaaatcatc ccaagatatg
    50881 tcttcttcct ctttttcttc ttggatgatt tctgcattcg gatctactgg ttttttcaat
    50941 aataaaatgg ttattcctgt gacaaaagaa ccagctaaca atgcaccaat gaatgcccat
    51001 ggtctggtca tcgtagcgac tgcaaacatt cccgatgccg gaatacggct atctgcgccc
    51061 caaaacatgc ttaatccgcc acctactgtc ccaccgactc ccgttgctat gacattgcgt
    51121 gccaagtcgt tcaatacgat agggaaacat ccctctgtga tctgacagat ccccatagga
    51181 aaagcggtct tcaatgtatc gatttcttga cgattgagta tatttttacg aatgattttc
    51241 cctaagaaat aagcgatcgt caagccgaat ggggttacca tcgaagctaa aatcaataca
    51301 gtgattggtt catagattcc ttctgaaaat cgtgccacac ccccttatcc agaataaatg
    51361 gtaatccaga aacagccgtg tatctgcggt aataagccta accaaacgaa aaatatgaga
    51421 agctattttt gtttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
    51481 tttttattta tgtttcataa tcaatatggt aaggatgcag ctccgtatgg tagaccgcct
    51541 ccgaatattc aatcatttcc ccaatttgcg tagacgattt ccgtatccgt ttgatgccga
    51601 tctgtgcgtc tgttttcaga attccttgtt cttcttctgt taaaaatact gccgagaaat
    51661 catcagaaaa tgagtcaata gtatatcctt ttttcgtcag caaacgatac aaagattctt
    51721 tgccaaattc ttctctgctg aattgcatca tttcttgtgg aagataatga acaaaataaa
    51781 tataaggctg attgttcaaa taatataagc gcttaaaaca aacagcttgc tcaccgaaaa
    51841 aggaaaacaa aggatcttcc ttctttatat ggaccaattg caaatccagc gtttctttcc
    51901 ggacttcata tcgggagttc tccaagattt gcgtaaagct tgccgctttg gataatcgat
    51961 tgtaaggacg attgcttaga accgtcgtcc cacgaccact tttctttgcg acatattcat
    52021 ctgatgccag cagttcgatt gcctttcgga cagtgatttt gctgacttga aacagttctt
    52081 ctaattcact ttctgtcggc aacatcgaac cgactggata ttttcctgag aaaatatctt
    52141 ctttaatttt atctgcgact tccaagtata gtacacgtgt ccttttcata acgtcaccct
    52201 ttgctttact attactaaaa gaatagcaca taaaaaataa aaaaagaaca caaatcacat
    52261 gtgagaagtg tgttcttttt cattcagctg ctttaattac aagtttacgc catttgaggc
    52321 aattacttct ttataccaat caaaagattt tttctttgat cgattcagtg tcccatttcc
    52381 ttcatcgtct agatccacat agataaagcc gtaacgttta ctcatttctc ctgttgatgc
    52441 actgacaaga tcgatacatc cccaaggcgt atagcccatc aaatctacac cgtctgctac
    52501 tgcattagcc atcgcttcga tatgacgacg taaataatcg atccgataat catcaataat
    52561 cgtgccatcc tcttccatat gatcaatggc tccaagaccg ttttcaacaa tgaataatgg
    52621 tttctggtaa cgattgtata attcattcaa tgcgattctc agcccttctg gatcgatctg
    52681 ccagccccat tcacttgcct caagaaacgg attttttact cctcccagca agttcccttc
    52741 tgaagttgct gcttgtggat cagtctcatt gattgccgta gacatataat aactaaatcc
    52801 gatgtagtcc actggatatt cttttagcaa ctggaagtct ttttccgttt gctctaattt
    52861 ttccggatcg actccatatt ttttatgggt acgcgctgtg tatgcagggt attctccacg
    52921 tacttggaca tccgtgcaga aaaaattgaa ttcttggttt tggatcattg ttgctgcctg
    52981 attgactgga ttggcatcga tgctgtaagt tgttgcatag ataatcatgc agccgatttg
    53041 gatatccgaa cgcaattcgt ggccgatttt tacggctttg ctacttgcaa cgaattgatt
    53101 atgccatgct tggaaaatat tttggtactc tcctgctcca ttgcttttta ctaatccttg
    53161 acttaaagct ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc aatatttgac
    53221 tttactgctg tatctctcta atactgtctg agcaaatcgt tcatagaagt cgatcagttt
    53281 tcgatttttc cagccaccgt attcttttgc caaatgaaga ggcatttcgt aatgagaaat
    53341 cgtgatcaca ggttcaatat catattttaa gcactcatca atcagctgat cataaaattc
    53401 taatcccgct tcgttaggag taccttcgtc tccatttgga aaaattcgtg tccaggcaat
    53461 cgaaaaacga taacatttga atcccatttc cgcaaacaag gcaagatctt ctttgaaacg
    53521 atcgtaatga tcgattcctc gatgattcgg gtaaatatat ttttcttgat caatcgtcca
    53581 gtcaaattct tcactgccga taatcgcaaa gcgctgctta cctccaggca tagcatctgc
    53641 aacggatagt ccttttccgt ctcgatcata agccccttct aattgatttg gcagcagttg
    53701 ctccacccca taaaaatttt tctgaaaaac gtgattgcat cctgatttcc tccttatttt
    53761 acaattgcca gaacatcttc gtttgcaata atctcttttt ggtctaattc aagtatatcg
    53821 gcaaatgcat cagtattcgt aatgacaact ggagtgatta cgctatagcc agctgctcta
    53881 attgcttcga tgtcaaaacg aatcaataaa tctcctgctt tgacgagatc cccttgtttg
    53941 actaagatct caaagccatt cccatttaat tctactgtat ccatcccgat atgaatcaaa
    54001 atctccgctc cgtcagtcgt tgtgatccct acagcatgtc cagttggaaa aatagtcgtg
    54061 atctctccgt ctgcaggtgc aactagttct cctacttctg gatcaatagc gatacctttt
    54121 cccatcacgc cagaagaaaa aacttggtct ggtacttcac tcaatggcag tactttaccc
    54181 gttaaaggac tttttaaggt atgccgtgct gaagtaatcg gttttcctgc attcgcatgt
    54241 ttgttttcta attgattgtc ttgtgtttgt tcgtcaaatc ctaaaatcaa tgtacctacg
    54301 aatgcaagaa caaaagcaat tcctgtagct attactgcta ctgtcacatt tgactctaca
    54361 ccatccaccg tattaatgaa agtcggaatc gtcaataagc tgaccaaact gctggagaaa
    54421 gcttttactc cggaaaatcc gataatagct ccaccaatac ctccagaaat acaagcggca
    54481 atgaatggtt tcttcaaagg taatgtcaca ccatacacag ttggttcagt gattccaaat
    54541 aacgaagtca ctgtagaaga aatccctaat gctcgaagtt tcgtatcttt tgttcgcaat
    54601 gctacagcta aagcagcccc accttgtgcc aagatggctg gaagaagcat tggcatcaat
    54661 acatcaaacc catattgttc gatattcaaa aacatgatcg gtacgaatcc ccaatgcatg
    54721 ccgaacatca cgaatacttg ccataaactt cccatgatta gacctgctac gattggacta
    54781 aatccataaa tcgagttgaa gagaccgcct aaagcattac cggcaaccgt cccgattggt
    54841 cctaaagcaa gaaatgttaa tggaaccatc actactaaaa cgacaagcgg aactaaaatc
    54901 atttgcagaa attgcggaat cacttttttt acgaatggct ctaatttact ttgaacccat
    54961 acggctagaa tgattggtat gacagaagaa gtataaccag atggtgaaag aatcactggg
    55021 atcccaaaga aactgatagc ttctcccgca ccagcaactg ctgtgatagc tggatacaca
    55081 agtgccatag caattgctac tgctaaaaac tggttggctt tgaacttttt agctgctgta
    55141 aaagcaagca tgacaggtaa gaaggtaaag acaccatctg ctgcagcaaa caatatttga
    55201 taagtaccgc tatcagctgt cagccagccc atcaccgttg ccaatgataa gaatcctttc
    55261 aataccccgg cggctgccat cgcccctaga aaaggtgtaa atatactaga gataatatcg
    55321 atcagacgat tcaaaatatt tttggactct tcttttccat cctccgaaac tgcaagacct
    55381 tcctcatcca cgatcgattg ataaacatct gctacattac tgccaatcac tacttgatac
    55441 tgaccgccac tttgtacgac ttggatcacg tccgggtctt cttttaactt ttgcgtatct
    55501 gctaaagatt catcttttaa tctgaatcgc aaccgcgtag cacaatgtac taaacttttg
    55561 acattctttt gtcctcctac cgcttcccat acacgacgac caactgcttg attatccatt
    55621 gttttttcct tctttctttt ttatttaaaa aataaaaacc cagattgaaa aaaacagtca
    55681 caaacattcg tgaacctatt ttttcaatcc aggttttgcc tgccatacag taacaatcct
    55741 tatgattgga tgcttttttt ctcacttact actctttgaa tatgaatcgt taaataaatc
    55801 tgctctgcga tccccatttc ctgtttttgc gtttgtcgaa gatactcatt gatccgttcg
    55861 acacaatgaa acgcttttgg atactttcct tgaactaatg cgtagagaaa ttcatcttgc
    55921 tcgtcagatc cttccccttt gagataacgc tgggcaaaaa actgcagatg agtaatgata
    55981 cgctgataat tcaaagattc ttcatcgaac acgatgccga agaatttact gatgatactc
    56041 agtatatctc ttaccattgt ggtagcggtc atcgtcactt gcatgtcttc gtttccttgt
    56101 tggctattca caaaatgaaa ggcaataaaa ccggcttcat cctcagaaag ttccacaccc
    56161 gtcttttgtt tcacgaacaa aagagcctct ttcgctgctt gatattcttt cggataaaat
    56221 ttttttgttt caaataataa tggattaggc agttggatat tttcttttgc tcgtgtgatc
    56281 gcataatgaa gatgatcggt caatgtcaga taaatattag gcgaaaattc aacttttagt
    56341 ttatcttcag ccagttcgac tacttgtttg acccattcga tctcctctgc cggaatatca
    56401 gccatcagct gctggaattg gtttttagca aaagaatctc ttaaaacaaa ttctttttgg
    56461 accgcttctg gcgaaatctc gtccccaatc tttttttgaa aagctagtcc ttttcccata
    56521 caaacgactt cttccccgaa tccatttttc gaaatgacga cattgttatt caaaatttta
    56581 tcaattttca ttttgcttcg ccctctcaag aaaaataaaa aaacctagaa ttcgcctact
    56641 gtttcttagc tgaattctgg ttttgcctgc cgtacagtaa caatccattt cttattcaaa
    56701 atcatcatac catcgaattt ttatttatgc aagccttttc atataaaaaa taaaaaaact
    56761 ggaacacaag tctatctaga aaacaaccaa atcagagatg cacctctttg cgtttctctt
    56821 tttttcagca aattggtttt tccttatcga ttcatatcct ttggctgttt ttaatctgta
    56881 acgacttatg tcccagtatc ctaaaatgtt tttgtctcta gattgaaagc agatctcata
    56941 tcatctgtcg catcttttat cgatgtaaca ctttccagtt ctccttgtac aactagtcgc
    57001 gtgacggcgt ccatgtcgcc acaagtaatt agtgtgacaa tttcttttcc ctcgacttca
    57061 ttcagtaatt gggtatctgt aggagcaact ttttctttaa aaaatgtttt gtatgtatac
    57121 acattttcta aatctgtcaa atagatcttg gcacccatct ctaaattttc tagcggtgta
    57181 aacagcaatt cagggttcgt tgcacgatgg cttgctaagg cataatttcc ttttcccatt
    57241 tcttgatctg gagataatgt tcctgctccg taaagtagtg acgtattatc caagcctttg
    57301 aaaaccggta aattgattga aactgaaggg actgcgatac tagcaatcac tggtagatct
    57361 gttcctctca tttgcgacct catgacccct tctgtcgtca ttggttctac cgcatcaaag
    57421 tcaaaactga catctttatc gttattcttt ttcagatctt cttttgttac attcgctata
    57481 gcatatttat ctcctgtctc tctgacaaaa taatccttga tctgttcatt gaaaatcaaa
    57541 gctagtccta ctaataacaa gaggaacaag aggatattga ctagccatct ttttgctttt
    57601 cctttttttt tcattttctc tccctccaga ctcattttac tgtttttctt ttgctctttc
    57661 caacagccga atgacacatt tctacaactc tgtgtatttt tttcgtacac ctgcgctttt
    57721 ttctacagga tatttcaaat tatttttgat caatttttct tcaatgatct catctagatc
    57781 caggtgaagg ttgtctgcca tcatgtatga atagatcaat acatctgcca gttcttcttt
    57841 gattcgttct aattgtgttt cttttacttc ttccggcgtt ttccattgaa aaagttctaa
    57901 caattcagat gcttccaaag aaatagagat agccaaatct ttttcgttat gaaattgtcg
    57961 ccaattacgt tcatctctaa actggttgac tttttccata ctattcattt gcttgccttc
    58021 ttccaatcac ttcttacttg attaatcata ccattttttt acataaaaaa tagatgatgc
    58081 ttttaaaaaa actcctgttt tcttttattt aaaataaatg tcatagaaaa tcaataattt
    58141 aggtagccac catgtgtatg cagtagcgtg tcttcttccc ttgaggcttt ttgtttttcg
    58201 atacgttctt cttcccaaac agacaacgct actagaatct ctcttaatga ttttccttct
    58261 tcggtgagcg agtactctac tttaggcggg acttggttat aggcttttcg atggatactt
    58321 tcatcttgtt ctaactctct taactgctgg gtcagcattt tttttgtgat cgtagggatt
    58381 tgcttgcttc tccacgccta aaataagcga tattgctctt ccctttatct ggtgtaagca
    58441 aagcagttag cattttgacg atcgtgggtt ccgcttttca cttcttatct acttagtatc
    58501 atgaagtata ctttcgtact aatactaagt agcggaagtg tcaactactt tgtttaacta
    58561 catagcaaaa gaaacagaag ggtgacaacg gtcatcacgc ttctgtttct tttgaatatg
    58621 ctgtgttgtt gtctctatct ccgtgtagac tttcctcaaa cttatccttt gacagtaagt
    58681 cagtaggctg atttgctctt tctagatgcc agatacattt ctacaaaaaa atagtgattc
    58741 tgttttgaaa attcgatttt tagttctgca ttttcgtaaa catccaacat ttttttcgca
    58801 ctgtatagac ctaacccgcc atttttttta gaggtttttc ctcttttgac catctccgat
    58861 agcgtcaaat tcgttggatt tgccttgttc gctatactaa aacgtgatcc ctctttttct
    58921 tccagcagat tgatcgtaat cggactctga tcggctacac gatgatcgaa tgcgttagaa
    58981 ataaaaatag ataaacagcg aataaaaaca accagatcaa tcgggattgt tttcacttcg
    59041 acaggaataa ctacttggat cgggattttc tccgattctg ctttttctag aaattttaaa
    59101 aagacacccc tgacagctgg ggatacaata tttgttagct gttgtatttg cggtttttca
    59161 aaaatagctg cagaatgatc ttctaatatt tctaaaaatt ccaacgtttc ttttttattt
    59221 tcctgaagag taatttttaa tgaaaacagt agacttttat aatcatgttt gaattcctct
    59281 agatgatggt aatattcttc atttatttga tacagttgaa gcgattcctg atgttgtctt
    59341 tcttttactt ggaaactgat caacataatg gaaaaaaaca aagaaaaaaa actgatgact
    59401 atcgacaaaa gagatagaag cagataatta gatgagcgga agtgaacact tcccaatacc
    59461 aagtaaatca cgccccagct taataaaata caaatacctc caaaatagtg ttcagttgga
    59521 taagtaaaaa gcaagttgag aaatctcgtc tgtttgtcta tactttttac taatagatag
    59581 aaaataaatg cgataagtag tgcaaaaaaa cttgctgttc caagatgtct ctttcctaaa
    59641 taatccatca gatcaaaaac cagaaaaaag ctgagtccag tgatagcaaa aaaaagatta
    59701 ctgatcaaac agctgataaa caggtttttc gtagtaaaat aaaaaagaaa catcaataat
    59761 aagccaacta ggtaaggaaa gctcacttta gggataggaa gtaaaagtgt caccaccatc
    59821 aaagctatac aaccaaagct gttttttact agcagttgtt tcgaaaagaa taaatagata
    59881 cctaaatcta aaatcaaagt aaaggtcaaa taaagtgtca ctgttgcgac catcttattc
    59941 ctccattgtt ttgtttcaaa atgtacacat atttttttaa taggagcctg catatgaaaa
    60001 agtattttat catagaagat aaccagtttc acagcgaatg gatcatcaga gaattggtca
    60061 atcatcatat cgagaatatc gtgcagctca cttctatctc tcaaatcaat cagttgatcc
    60121 agttgggcac tattgataat cacgatgttt tctttatcga tatcgacttg aagcaatcac
    60181 tcacaggtat tgatgtcgcc gaacaaatac gtaaaaaaaa tgcacatagt aatattgttt
    60241 ttttcaccgc ttatacgaat tttgcagtag agatcatcaa tcggaaaatc cttccgattg
    60301 gatatatcag aaaagacagg aatttatctg aacaaattcg tcaagtgcta gaacagatca
    60361 attggaaaga acagcagatc gtaaatgtcg ataaaatctt tattgatcaa aaaggtggaa
    60421 gtctcgtcct gtatcctcgg gaaattttat atattgaaag tatcaaaggt ttgaaaaacc
    60481 aagttatggt caaaacaact acagaagata aattagtgaa tacttcttta aaacaattga
    60541 agacagagtt agaaaagtat gcttactttt tacttttaaa atcttactgt atcaacttat
    60601 cgcaattgca gaaaattgat tctgctcatt atgtgttaga atttttcaac ggtgactcct
    60661 tactcgttgg tcgaaagatc tttgaaaaga caaaggaacg tttccatgac ttccaaaaaa
    60721 ctgcttcgag ttgatgaata agagctgaga cgtaagcata gcagatttca gggagtgggc
    60781 cttgaatcgg ctccactccc tgaaatcgtt gatatgattt tacgcttcct cttctgtagg
    60841 agcatctgtt aacgaccatg tgaatgttga aacgtagttg tctacagcat aattggatac
    60901 aggaacaaat aattccactg attgtctccc ttgttccaca ttctctccta atgaataaat
    60961 ccatgtgcca atcccttcat ttgctcctgc agttactaga tctactgcct gatctgctgg
    61021 gatttcgatt ccttcagcgc cagttccaga aacggtagat ggcattccac tgctattagt
    61081 tgcgctattt cgaatcacgg cgtttttgaa tttcaccttt gaaccagtta gtttattcgt
    61141 atcagatgtt gtaccaataa attctgttcg tttgactgat aatttccaac cggcataatt
    61201 tccgctttta tccgttactt gtacgaaatt ctgtacttgt gtttgtgctc cagtactgtc
    61261 cgttccgctt tgtaatgcag catatactgt ttttgctgta cctaacttga ttgttccaaa
    61321 atcaagatct ggagcttggt caatcgtcag taatccggat gttccctgat ttggttcgtc
    61381 aggtgtaaaa ggagtatcag gatcatttgg atcaacagga ggaactggaa caacgtcttc
    61441 aggatttgca gcatctggtt tggtcacaga aatcgttcct tctgtagtgg ctgagctctt
    61501 attctcagca aaaacacttt gtgtacataa taatagactt ccagctagaa cggtacttaa
    61561 tgcaattgtt tttttcattt ctttttcctt ctttctttta aaataaataa tccagcaaat
    61621 aagccaataa gaatgactcc aacaagtatc agccatcgcc agtcaaatgt ttctggctga
    61681 ttttttaaat caatatcctt ttcattcaat tggtccgctg catcatcagt tatttcaaaa
    61741 tcggtatgga gattccagcg tttttctttc caagcaccct tcacaaccgc ttgataatgt
    61801 cccggagcta atttttttcc ttctaatggg acaagtaaat caaaatttgt attgggtgct
    61861 acatctattt tttctttttt tattgaaagg taaggctctt ttgtttcttt tttgtagatt
    61921 tcaaccgatg tagtcaagcc gaacatataa gaagcatttg gattttgaag attcgcctta
    61981 atcacatttc ttccatcgct ttgatccgca taagcttttt ttaatttcaa atctggggaa
    62041 gactcttttt cgctatttca aatcaagatt gctttcactt gagaaaactg attgacgaca
    62101 ccaactgtac ttttgttaga aacgttctct tttttttctt gaaacgtgat ccctcctgcg
    62161 accacaccag ccagctcttt ttctggcgct ttcagtgaaa gtgtctcagt gaccgcactt
    62221 tgaggaggaa cagttatttc tcccactgtt tttgctaagt ttttcatatc ataacttaat
    62281 gtttcatcaa atttactttt tgattctcca tattcgatca caccgtttga attcgtcgta
    62341 gcggagtgga tcaatgtctc aactgttact gatttatctg ttcggttaaa gaccgtgtag
    62401 gggatttcct gtgttttccc aggctccatc cttacattta tatatgtttt gttcttatca
    62461 atctgattag ccggtaattc aaaatctacc gcaaaattga attcggaggc atggacaggt
    62521 gtctgccaaa ggatgaatcc aaggagaagg atcataagta aagagcagtt gtttcgatga
    62581 cttttattcc atattcgatt catcaatcat cctccttgat ctttattctg tcggcgcatc
    62641 tgctaatgtc catgttatct ctgctgagta attaccagct atctgatttt ctttggttaa
    62701 gacaagttta aacgcccgac cgttatttaa atattctgaa taatctgctt gcacattcgt
    62761 tgaattttgt cctctgacaa tttccagttc ttcttcattc actaattgat actcctctcc
    62821 tttcttgaaa tataggacat cctgcataga aaatcctgca tcatttgtga aaggctgaga
    62881 taattttatc gttaagatcc acggattttt cgtttctcgc tgatcataaa tccataattt
    62941 ttcctgtata tccatttggc caaatgcttc ttgtgtcaaa ccagttgctt tgatctgacc
    63001 aaaatcgagt ttggaagggg caagaaatga gagaccacct tctactacga tcttcgtagc
    63061 aggtgcaaaa ggcggggcat catgataaac aactggcgta tcacttggat tgtgattccc
    63121 aatttcatcg ttggtacttg gcttgttctg cacaccctta gcagtagctt ctcctgcttg
    63181 gtcgtttaat acaatttcta atacatctcc tgcaaaaagt tgattttctg ggatagaaag
    63241 aacaaattcc cctgtatcat caatagttgt ttctgacaaa acagcttgat tttttttcaa
    63301 tgaaaccaag gctcctgctt cacctgtacc tgtgatcgtg cgagaagctg cattgatcaa
    63361 tgtggtactt aaattagcag gttcaggagg aatcacatcg actgtcgttt tggcagagtc
    63421 cagttctgtc acttcttggc cacctcttac ttttttagca gcaacaactt gcacttgatc
    63481 ctccgttttt aatctctcat cattgggtac ttgaacttcg aacaagccgc cttgttcgcc
    63541 ttctccccat gggttatatc ctgccccttc attttcatct gtttgtcctt gcgtgagagc
    63601 agtaatttct acagatgatt tcgtcgtatc tgcgtaagta atacgtaaag tcacttctac
    63661 ttcgcctgtc caagcatctc ttggctttgc ctcgacacct tcgggaacaa cgacatgccc
    63721 atagattttt ttatctgcat tcgttggcac tcttaaggat tcaatcatcg ggagctgatt
    63781 attggcactg attcgattat aatttttcat tccggcacct gtctgagcaa attcatttgt
    63841 taaacctgca ctagggcttg atacgaaatt gcctaagtcg gtcccattga tcgataagtc
    63901 tgtcatatca gaaaaataat ccgggtcttt ttcaaagtta tcgaatgttg cgcctgtttt
    63961 tcgccataaa gcgatttctg aatgggcgat ctttaaagaa gaatctccat ttccttggaa
    64021 tacccagccg cctatgccat tggtctcgct tccaatccgt ttgttttgga aatcaaaata
    64081 ggtaggagat tctaagtcca cattcaacac atccccactg atgatgccgg tcttatcttc
    64141 ccctgttttc ccgaccatga ctaaataagt tttttcacct gccgtaaaat ttaaatcaat
    64201 atctccagtt gtatctacag ttgctccgga atctgcgcgt atatcgatat ttgatttttc
    64261 tccagtgact gtaaaatcag ctgaacctgg actttctaga aaaccagaag cgtcataaaa
    64321 ttggatcgct tgatttcctt gattgagtct gttcacgtaa gatttgccat cacctgtatt
    64381 tcttaccctc aatgagcctc cattggttac tgtcagcttt tgcgtaccat tctcaaaacg
    64441 aactgcgctg gaacgcccac tctgcttatc gatgtcgata actgatccgg ctccttctac
    64501 gatcaaagaa aggccttttc ctacaaaacg catcccattg ttcccgggtt gatcgtcatc
    64561 tccagccatt ttgaccgaga tttgtccacc acttgataca tggatttgac tagaatctcc
    64621 ttgcaagcga aatccgcccg tagttgctgt attgatggtg attttcccac cattcgatac
    64681 agtgactttc gatcctgaac ctctcatttg gatgattccg cgattgttgt cattggtaat
    64741 attgatcttt gtatttaccg aaaattcgct tcccgaacca ctgacttcaa acatgacatt
    64801 accaggtagc gtatcgactg cctctccttc atcgctaacg ccatctgccc gatcataatc
    64861 aagagagacc tttgaaccgc ttaagatatg gattcctggc gctgtttttc cactaccagt
    64921 ctcatctcct gaatctagaa aaccgtagaa tttccctaga tacggtccat aaagttgact
    64981 accattgatc acattcaatt gatagcttct acaaaaatag gctttacccg gagcggcatt
    65041 cagtccttcc ttcactggtt ggacatccaa cacaccggaa acaccatcta atgtgacagt
    65101 tgcacgagga gcgtaaatca gtcctaattt ggacccatca tacaaatcaa gtgtcccggt
    65161 aaattttatt tctcccgtaa aatgatatct gcttccccaa aaatcggtca tataagcaat
    65221 gataatggca tttccatcga tatttggtgt caatccacct ggatgtgcag ctgttccttt
    65281 aaaattctgg aactcgatcg actgaggtat ctcattgata taaatatggt gtgtgccgaa
    65341 attgtgagcg aatccaccaa aatcaataac gatatctcgt ttgatctcta attccctgtc
    65401 taaagtcaaa ggctgaatca gtcggatctt agttactgat tcgtctgcga gaagctgcgc
    65461 cagttcttct gttgtggcag cttcgctgta cccatccgct gaaacagctc ttgccagtgc
    65521 cgcttcactg ctatcagacg tttgttcagc tacttctgag gtctcacttg tcttattttc
    65581 ttcttgcctt ttattagtag aagcctcact ttgagattct gtagtttcag aagtaatcga
    65641 ttcttctgaa aaagtagtct tgtcaaaact ttgattctct gattgcgttg attcgtttgt
    65701 tttctctaca tcggcaaaaa cggatgtcac catcaagaga gggaagacaa acaaaatcga
    65761 cgcaatcagc ttttttacac attgattcat tctccaccat cctctctgac gttttgacaa
    65821 ttttatttta tcgttttaat ctatttccaa ttgtactttc atatttaaaa tatgtatttt
    65881 tatggaaaaa tttttctatt aggtgtaaat ttcttttttg tacaaaaaaa aggctgtgac
    65941 aaaagtcttg tcacagtccc ttatcccgaa caagcggtgt tctcccgcca actcctcggt
    66001 cacaacctcc attttaacaa taaacctgta aatgtgaggg caatactttg attgtcacag
    66061 gtaatttatc cccttcatct ccatcaacat tcgtcgtcaa ttcttcctca tcagctaatg
    66121 acacaactaa ttgctcacaa gtaaggtagg ctagattttt cgtagattca tctactcctt
    66181 ttaataggtc tggcaatgtt ttaagcgtat cgatcaaaga agaatctttt aaataaacca
    66241 aatggagctt accatcatcc acttttgcat ttggaagtaa tgtttcaaaa ccgccgactg
    66301 agttagttag tcctatcagc aaggtactgc tctcaatttc ttccttcttt ccatcaactt
    66361 ctacgtgaaa cgagtaggat tgtgtggaag ctaactgttt gattcctgac atgaaataag
    66421 ctagttttcc aaatttcgtc ttcttctccg catctacatc attgattgat tcagggatag
    66481 agccaattgc gacgacattc ataaaatagt cgtcatttat ttttccaata tccaatggct
    66541 tgactgattc aatcgaaaaa tgattgatcg cttcctcagg ttctagcggt atccctaatg
    66601 ctcgagccaa gtcattcacc gtacccaatg ggaaaaatcc gaaatttggc cgatgttctt
    66661 gttcagcgat tccactgatt ccttcgttga ctgttccatc tccgcccatg acaaacacac
    66721 tatgataccc ttccgtagcc gcttcgcgag taaagttttt tgcatcccct gctttttttg
    66781 tatgcaagac gaccacttca tcaaacaccg attccaattt tgctatcgct aattgctcaa
    66841 attcctttgc ttgttcaccg cctgaactgg ggtttaccac taataaaact tttttcatca
    66901 ctttctttcc cttctgattg atttctttaa tataacgata gcataaagaa aaaaattagg
    66961 ctgtgatgaa gtactgttca tgaaacattc atttattttt tgacaagata aaacatccat
    67021 actatgaaac tgaggaaaat taaaacaatg atataggaaa taaaagaaaa tgataggcat
    67081 tgtataccta ataaagtaaa aatcttaggt ggcagataaa aacacaaaaa tagcaagaga
    67141 atgtaatagc gtttcttcca ctttttgtta tcagacctta tagcgatcag aggaatacca
    67201 aatgcataac tgaaaaactc ttgatgtact gttaatatgt cacggccttg aataataaat
    67261 gcgacaatcc ccgctataat tagtgtgcct aacaccaaaa cggaaaattg atgaatatat
    67321 tttatataca aatgatccat tgtacttcct gcctttttat ttgtgagaaa aacaaatccg
    67381 tatacgtgaa taacttctag aaataaaaga aatttgcaaa accactccat ttatctactt
    67441 ctacgtgcat tttttgatat tgttttcttt atttaagatt atacagaaaa atatctaggg
    67501 tcgctggatt ctaagtaaaa aaaattttta aaataaaaag aactaaatca agtagtaatt
    67561 aaactactgt tgatttagtt ctaataaaaa gatttagatg aacagtttta ttgatttaaa
    67621 tagttcatat tttttattta taccgcttat ttactacttg ctgtactaga atctgaagtg
    67681 aatgtactca aaacattttc aaagtctttg tctttgattt ttacattagc gtctttcaat
    67741 acttcgccaa ttactttatt ctggaaggta ctgtcactta attttgtgtc tgttgcaatt
    67801 tctttcaatt cttttttgta tttatccatg tcgttgcctt tgttttggtt tttcaccatt
    67861 ttaaccacat aatattccgt agtataagtt gaagcattgg tagaagtgat cacatcagaa
    67921 acttgaccat ctttcaactt aaaggctgct tctttgactt ctgccggaac cgttgtagta
    67981 gtggaatcaa atttcacttt tccgccatct tcttttgtcg tagtatcagt tgacttgtct
    68041 ttggctaatt tagagaaatc gtctccctta tcagcagctt tcttcacatc ttttgcatca
    68101 tcttcacttg ctacttggat aatttgagct tccacttctg gatggaaaga ttcccaagcc
    68161 gtttttaaat cttcatcggt caatttgata tgtttcttta gaccttcttg aaaggctaga
    68221 ctttgtttga tactgtcttt gaatgtttgt tctgtgtaac ctgcagattt caattgtgaa
    68281 tcaaatgaat cacccaattg tttctttgtt tgatcatatt gagaatcgat ttctttggtt
    68341 gatacgtcat ctccatattt atcttcaaat acttgataaa cgatcaactg ttgcacagcg
    68401 gaatctgctc cttgcatttt tttcaagcta tccattactt cttgttcgct gattgtaccg
    68461 cctttataac taacggctgt tttttcaccg gcattcgtac agcctgacaa taagccagtg
    68521 cctagtacga tggcaagtgc taaaaatctt tttttcattt atcaattctc ctacttttcg
    68581 ttttttgcga attgctaaaa acaaaggtgt tcaagatgaa aatcatcaag aacaccttta
    68641 ctaatctgct cgatacgtcc tgaatagata taaggctaca caatccagaa agttcgctca
    68701 gcagaataaa tgtcaagtag cacatcacac cttaatatac tacttctttt gttaccgttt
    68761 ttcaagtcta tcttgcgttc cgtatttaac gtaactaatt cttagaacaa atgttatcga
    68821 ttttgttttt cttcttccgc tgctgggata ttcaaatcag acacttttat gttcgatttt
    68881 ccagacattt ttttgttgtt tttattatga tatattttta tacaactaaa aatgaatata
    68941 ttaaaactac caaaaataac ttttaacgct gtttcattat atgttttttc tattttatat
    69001 aaacttttgg atacttaata ccaaccggtt ggattgtacc aacatgagaa tgtgtcagtt
    69061 ctatttcctg tcgcacttgc ataggcccat actccttttg ttgcatttgc tgatcgaata
    69121 gagtttccaa ctttcgcttt tgcaccgtga cgattataga cacgactata gtggttagaa
    69181 tgacctgttt ttctcgtaca agttgtatca ctataataaa cccatcttcc agagctatga
    69241 ttaattgctg ttctagaaat ttcccggtct actggtaaat ttgacttaat aatattacct
    69301 ttagcatcaa aatgaatctc tcctggcttt aaagatttgt aatagatttc accgtgaact
    69361 gaatttgctt ccacttcaga tgacatagcg tttcctccaa caaaacctat ccctagtgca
    69421 caaataataa ctgctttttt cataaaacga catcctttcc tttttgattt ttaaagcatg
    69481 atcttcttgt ttcctatttt ggtcagatat ggatcatgcg tgactacgat aactaacgta
    69541 ccttgggaag cttgttgcga taagatttca actactttat ccttatttct tttatccaat
    69601 gacccagtcg gttcatcggc taaaatgatt ttgctattct tcaataagac tctcgcacag
    69661 gcgattcttt gcttctcacc accagataat gtgtagatag gcgtacttag cttcttgttt
    69721 atatttactt ttttcaatgc ctcatacttt gctgttcgct tatctgaaac atttaaattc
    69781 gaaaacttca atccaatatc taaattaaaa tcgattgttt catattcaat caacccaaaa
    69841 tcttgaaaaa gaaaagatat ggtatttctt tttatcaagc gcttctctct cccagttaat
    69901 tgtttcgtct cttgatcgaa caaatagaat tgtcctgaac tgaactgatc attcaatcct
    69961 aatatattca ataacgtact tttacctgct ccactttctc caacaacagt cacaagatcc
    70021 ccagcatccg cagtgaaacg atccaaatga agaatcgttt tgtttcgaat gatttttttc
    70081 gcacctttta tcactatcaa cggctccaat tgttaacctc cattcatcag caataactga
    70141 cttttctttt ctgtagacaa aataaaaaca actgaaagaa taaaatcaat gaagctaaag
    70201 aaaaaaatat taaaaagata tttaggctct ctttctataa gaaaaaggaa gattacactt
    70261 gtcgttcccc atttgatgag gttcatcatg aagaaatctt tatacgtttc aaagagcgaa
    70321 taaccacttg aacgaaagat agcgattttc cgacaattgt agacaaaata tatgcataac
    70381 atgacatagc tcaacaagaa gaaagaaaag aaagtgagta aaaatatctg tatcaagtaa
    70441 tgaaatgatc tgcttaacac tttgacagct atctcttcag tctgagtata cggaaatgat
    70501 aactgcaagt tatcacgtaa ctgactttct tcaatcagct caaataacct ttctttcgac
    70561 aaagagccta tcttgatctt taacggtgga tagatttctc cgctgagaat atttctttcc
    70621 aaatacatac tgtttttcaa tgtcagtatt tcaacaaccg gataatgttc gatccagggg
    70681 atagaaggaa taaatgtata aatgggttga ttgtttttta taaatagaaa tttcggttct
    70741 ttttcactga tttcttgata atattctgtt atttgcgcca ggtcattttt taaatctgta
    70801 tgtctatatc gttcagggat caatataaca gggtgcgttt ctttttcggt tatttggatc
    70861 aaccttccag agacatcaat caaaggaaat ttcttaagat aattcagatt tatttttatc
    70921 cctctgccaa actgttcatt ttcttcattc aagtaatctg atacattgac taatatactt
    70981 ccgtttttat tcaaagtttc atataattcc tgatctgctg ctgatgcaaa acgattggaa
    71041 taaataaaat ccacgtggtt tttcccgatg gttactggat aaaatacatg atagtcctca
    71101 ccagcgattt cttttggtgg ttcatgggaa acagctaact gtgaagaaga aaaaacgata
    71161 gttgctaatt cgacattggc aacggtcaaa agaagagaaa acaacaatcc aaatgtaaaa
    71221 agaatgtatt ggatatagtt gaacgcttct cttaacgaag cgattttttc aactaataaa
    71281 agagaaatca aagagatacc atatattccg ctgaacaaga aaacgtacat ctggaacaaa
    71341 taaagaaatt gctccttata atacataaaa ataaaaccac caatgattcc tgcaattatg
    71401 aatgtaataa tgattttctt tttccccaat aaacaccaca atacttgatg taatgaaaag
    71461 tcattattca acaataaact gatttcttta ttcatcaata agaaaaagga tgataacaaa
    71521 aggaataaaa gaatcaatcc aatagttaaa tacactcgat aattatatac gtctgtattt
    71581 gaaataaccg aatacgaata gctattttca aagtcttcaa aaatattagt ggcataatca
    71641 gtgtgaaatg cttgattgaa tcgatcagtg aatagtgaaa tgaaccgttc ataggcttct
    71701 ttttctctcg tcatcaagaa aaattcacct tcaaaatctt ttattttgat cgattcttca
    71761 atggacgtta cgcgaaagga ggtattagaa tagggtacct tctcgtgaga agatttgaca
    71821 tccccttgat aatacaatgt catttcaagc tttggctgta tcttgaagtt ccaagatgta
    71881 tctacttcat tggattcatt tactgttttt ttcatataag aaagctcagt ttctgatgag
    71941 acattttcaa ataccatgat tagctgctca tattcttctt gcccaataaa atgttgtggc
    72001 aatcgaatcg gagtataagt atctgtttga tggaaaaatt catttgtccg atagagtacc
    72061 tgtttattta tactaaccaa ggcaaaacca ctcatgatac cggtcactat gattgtgagt
    72121 aaaactgtga actttttttt gattatcacc actctccctt acttaaatga ttctttgata
    72181 aaatcatacc gtttttcatc aaaaaaacgt atctatcaag tgtataggtc atgtagaata
    72241 aatctatata ctgtagaatc gaaaggagaa gaagatgaac accggagaaa aaatacgaac
    72301 ttaccgcaga aattgtaagc ttacgcaaaa agaattaggc gaaaaaattg gtgtttccga
    72361 taaaacgatt tctagttggg aaaattctcg tacaatgcca gaccttgaaa tgctgtcact
    72421 tttacatcat actttaggtc tgccgattga ttttccttct attgaatcag ctaccgaatc
    72481 aatagaacca acattgccag atgaacctct tgttaacaag aaatcttatt catcaaaaca
    72541 acccttctca ctcaaaaaga cactatcttt ctttctgctc gtcttttgtt tggtctatct
    72601 ttttcttatc aattatcaat acaaaaatga aacgatcgat cgattcaatc cttttttgaa
    72661 accacaaatc ggctatacca cactccctgt aggatacgtg gacaattcca tcgaacgttg
    72721 ggtatgtaat gatatctttg gccatggtac atggctgtct tttcacgctg gagaggtaga
    72781 aaaagagaaa aaccatgctg taatcaaaca caaaggaaaa cacgtcatga acgttcgcct
    72841 gctcacttgg gaagccattc cctcagatta ccaagaaatc ctcagccaag actatgacga
    72901 cgccttaaaa agaagccctc aggagccaga taggcattaa gtaggagatg aacgtagaga
    72961 tagaaaaaat caatgcacat gatcttatcc aaaaaacagg ggtacaaatt aattgaaaaa
    73021 gccttgagat gaaagttcct aaataaaaaa gttcagaatg ataggtattt ctggcttata
    73081 gtataatatt tacgccacag cctcccaatg attctttttg gaatcataat caaggatgga
    73141 cattcacaaa aatattttgc tcattcgatc cgaatagatt aacgaccaat ctagtaagta
    73201 atatagattg gtacgtagtc aagctgcaca taatttagat caattttagt tcttttttat
    73261 atttctatga tctgctcatt tacttttata tgatggctta cagtaacata aataaatatg
    73321 ataatcgctg gtagtttatc caagagtact caataaaata caaaatggat tgttcaagcg
    73381 cctatcataa tgataaaatg aaacaaataa acgatacaag gaggaagaga ctttgagtaa
    73441 tggcagaaca attttttcat tgtaaaaatt gttcagttgt taatctatgg gatttcggta
    73501 gcatgggcat ttttttatag aggaatcaca ccaacctggc cgttttatct cttgttagtg
    73561 ggaatattag taggaaattt tgcccctctt cactatcgta cgaacttttt tccaactgat
    73621 tctggccttt ttcttaaaac aaagacgcga aaattagaaa acattattga aatgacaatt
    73681 acctgtataa tcattttctt agttatattc ctaaacgtct tctaataaga atctcttaaa
    73741 attagcttgt tacagttagt tacctagttt atataacaca gttatcttat cttccttatc
    73801 aacatctttt gccaactctt tgtcttaaat tcgaaacata taagcagagc tacgaacacc
    73861 aagactgttt cggctctgtt ttttgcatca aattcactct tatatagctg ataatctcgg
    73921 ctctttaaaa gaaattattt tcgcttcatt ttattataaa aaggctagga accttgatgt
    73981 tatcaggttc ctagccttta tttaagtatt accccacaga cccttccata tagattttta
    74041 aattacacac tagtttaaac taaaacaaac aaggaaaaac ttaccattta atcaaaaaat
    74101 tagctcatat gaatatatta gaatgtaaat tagactggtc aaaatctggt caaaaaagag
    74161 gacgaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
    74221 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
    74281 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
    74341 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatacagtc
    74401 ccataggtaa agtgccacag gactgtatat ttttttattt gagattacct gaaatgattc
    74461 catttaattt ttatctcatt ttttcctact agttagtatc aaatactctc ctattttctc
    74521 aataaactat tttgggttac atggtatctg aaacagctaa aaccttgtca ttatagcaaa
    74581 aaatcacttt atctccgttt catttcagat acatttcact taagcggatt ccaaaaaaac
    74641 aaaagaaaaa ggaaccccta gttagcctat gctttttatt ggttcctctc aaaacacggt
    74701 catataacaa cggggtgggg taaatgaatg aaaaaggtgg aggaaacatt attcaatagc
    74761 tgtcttaaat acttcgttat aaaatgtgaa caaataaaag acatctatcc ctattttcta
    74821 cacttccact tgactaccat ctttgaatcg tacaattttc ttaactcttc tcgtttacct
    74881 caattcattc tcaagtggaa tcaactcatc atctaacatt ttacgccatg ctcgcttcat
    74941 ttcttgtggg tatttaaatg gcttcgcttc tggatgtttg gctttatatc cttcgtaatc
    75001 cttactgttg ataattgctt tcatattagg aattggatct tcacctggta gatattgcag
    75061 attagaagca tgtaattctt tctcagaaac aaaccactca ttggaaaaga catgaatcaa
    75121 atcatcactc gcttgtgtaa aatagcgacg tttaatgaca aagatatctt cattactatc
    75181 taaagtcccc acatcaaatg catttaaaat ttgtcggtaa acttctttga ctccttctgt
    75241 tttattcaat ttagccaaag ctttttcaat ttcttctcgt gcatctgaac tattcgcagc
    75301 ataagttcct agtaattgat caatataagt tgaatcaata tcatacaact tagtggtatt
    75361 ttcactataa aattcgatcg ttgaaaaatc ttctccttct ttttctggtt cttcctctat
    75421 taatgaaccc ttcacggtct cgtacacgcc aatttgttct tccaaaagtt gaatttgatt
    75481 gttcaaagtt ggagatttca ccatgtcatc attgtaatcg tcataagtca ctaacgattc
    75541 ataagcacga ttcaattctt gaaatgcttt gacgtactcg atacggattt caagtggcgt
    75601 atgttcatca atggattcca tggatgaaac ctcttttaac ttaccatatg cttgtttgaa
    75661 tctttttttg gattcctgat aacttggata aatcaaacct gattcttctt ttgcttttga
    75721 atacagtttt gtcgcatcct cgacattctt ctccattgtc gctggcttac gaaacgtcac
    75781 aatcaatcct tttgtcttat caggatacgt acgattcgta cgtgaaaaag cttggatcaa
    75841 tcctgcatac tctagattac gatccacaaa tagggtttga atcgtagggg cgtcaaaacc
    75901 tgttaataga cgatccacta caatcgctaa atcgatttgt ttcccaaatt gtttgaattc
    75961 cgctcttttt cgtgccaaac gattattgat gtctccgtta taacgctcaa tgtctgctaa
    76021 tgaccaggaa gtatcataat actgattata ttcctcaata attttttgca tttcttcttg
    76081 ttgggcactt gaatctcggc tattttcttc taatgaatag gtaatagcga ttcgtggaaa
    76141 atcaggatcc tccatcgttc gtccttcacg aatgagctga ttgggaaact cttttgttaa
    76201 ccaatcaggc tctttcgtca tctctttgat ggcccgataa tactttttgg ccatgtcaat
    76261 ggaactcgtg gttaagattg cagatttcgt tggcctgcca tttcgaaaat caaattttgt
    76321 ataggcatta tctggacgga acattttatg aatgactttt tggatatgtt catctgcttc
    76381 aaaaacagct gggtccaaat aactttcttt tttgacaggt tccatttgat caatcatacg
    76441 attgatctgt tctgatgaat actctgcata tgtttctact tcttttaatt tccgatagat
    76501 tttattctct agtgaagtcg gctccacggt gttttcatgc tctacttgaa atcctaagac
    76561 agagccatct tctagcgcat tcttaatggt ataggtatgc aagacctctc catactgatc
    76621 atgggttgta cgtgccaatt gccctttggc ttgtttccga ttctcaggaa aaatcggtgt
    76681 acctgtaaag ccaaaccaag tggatttcgg aaacattttt ttgatttctt ccatattttc
    76741 agcact
//
LOCUS       DAFCNJ010000008        66049 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000008 DAFCNJ010000000
VERSION     DAFCNJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.008"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(28..726)
                     /locus_tag="LNE35_000691"
     CDS             complement(28..726)
                     /locus_tag="LNE35_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBK6909873.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="LNE35_000692"
     CDS             complement(719..2071)
                     /locus_tag="LNE35_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBK6909874.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="LNE35_000693"
     CDS             complement(2294..3694)
                     /locus_tag="LNE35_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBK6909875.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="LNE35_000694"
     CDS             3855..4592
                     /locus_tag="LNE35_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBK6909876.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="LNE35_000695"
     CDS             4717..5196
                     /locus_tag="LNE35_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBK6909877.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="LNE35_000696"
     CDS             5193..5960
                     /locus_tag="LNE35_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909878.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="LNE35_000697"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="LNE35_000697"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK6909879.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="LNE35_000698"
     CDS             complement(6863..7465)
                     /locus_tag="LNE35_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6909880.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="LNE35_000699"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="LNE35_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="LNE35_000700"
     CDS             8146..8730
                     /locus_tag="LNE35_000700"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBK6909881.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="LNE35_000701"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="LNE35_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBK6909882.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="LNE35_000702"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="LNE35_000702"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBK6909883.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="LNE35_000703"
     CDS             10631..10810
                     /locus_tag="LNE35_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909884.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="LNE35_000704"
     CDS             10832..11857
                     /locus_tag="LNE35_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBK6909885.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="LNE35_000705"
     CDS             12032..13276
                     /locus_tag="LNE35_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBK6909886.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="LNE35_000706"
     CDS             13429..14376
                     /locus_tag="LNE35_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6909887.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="LNE35_000707"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="LNE35_000707"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBK6909888.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="LNE35_000708"
     CDS             complement(15278..15520)
                     /locus_tag="LNE35_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909889.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="LNE35_000709"
     tRNA            15641..15713
                     /locus_tag="LNE35_000709"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="LNE35_000710"
     CDS             complement(15832..16470)
                     /locus_tag="LNE35_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6909890.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="LNE35_000711"
     CDS             complement(16593..17225)
                     /locus_tag="LNE35_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK6909891.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17576..17875
                     /locus_tag="LNE35_000712"
     CDS             17576..17875
                     /locus_tag="LNE35_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6909892.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18370..20535
                     /gene="acm"
                     /locus_tag="LNE35_000713"
     CDS             18370..20535
                     /gene="acm"
                     /locus_tag="LNE35_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBK6909893.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20807..21292)
                     /locus_tag="LNE35_000714"
     CDS             complement(20807..21292)
                     /locus_tag="LNE35_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBK6909894.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    21382..21727
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21772..22413
                     /locus_tag="LNE35_000715"
     CDS             21772..22413
                     /locus_tag="LNE35_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6909895.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22432..23067
                     /locus_tag="LNE35_000716"
     CDS             22432..23067
                     /locus_tag="LNE35_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6909896.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23085..23900
                     /locus_tag="LNE35_000717"
     CDS             23085..23900
                     /locus_tag="LNE35_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6909897.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24219..25655
                     /locus_tag="LNE35_000718"
     CDS             24219..25655
                     /locus_tag="LNE35_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6909898.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25844..28291
                     /locus_tag="LNE35_000719"
     CDS             25844..28291
                     /locus_tag="LNE35_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK6909899.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28521..29588
                     /gene="rlmN"
                     /locus_tag="LNE35_000720"
     CDS             28521..29588
                     /gene="rlmN"
                     /locus_tag="LNE35_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBK6909900.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29781..30884)
                     /locus_tag="LNE35_000721"
     CDS             complement(29781..30884)
                     /locus_tag="LNE35_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6909901.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31437..31985
                     /gene="aac(6')"
                     /locus_tag="LNE35_000722"
     CDS             31437..31985
                     /gene="aac(6')"
                     /locus_tag="LNE35_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK6909902.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            32531..34192
                     /locus_tag="LNE35_000723"
     CDS             32531..34192
                     /locus_tag="LNE35_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6909903.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(34262..34609)
                     /locus_tag="LNE35_000724"
     CDS             complement(34262..34609)
                     /locus_tag="LNE35_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBK6909904.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            34693..35634
                     /locus_tag="LNE35_000725"
     CDS             34693..35634
                     /locus_tag="LNE35_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909905.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            35635..36678
                     /locus_tag="LNE35_000726"
     CDS             35635..36678
                     /locus_tag="LNE35_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909906.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            36684..37754
                     /locus_tag="LNE35_000727"
     CDS             36684..37754
                     /locus_tag="LNE35_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909907.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            37744..38697
                     /locus_tag="LNE35_000728"
     CDS             37744..38697
                     /locus_tag="LNE35_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6909908.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            38709..39719
                     /locus_tag="LNE35_000729"
     CDS             38709..39719
                     /locus_tag="LNE35_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6909909.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(40004..40825)
                     /locus_tag="LNE35_000730"
     CDS             complement(40004..40825)
                     /locus_tag="LNE35_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK6909910.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(40922..41200)
                     /locus_tag="LNE35_000731"
     CDS             complement(40922..41200)
                     /locus_tag="LNE35_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909911.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            41468..43477
                     /gene="metG"
                     /locus_tag="LNE35_000732"
     CDS             41468..43477
                     /gene="metG"
                     /locus_tag="LNE35_000732"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBK6909912.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(43956..44543)
                     /locus_tag="LNE35_000733"
     CDS             complement(43956..44543)
                     /locus_tag="LNE35_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909913.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            44718..46040
                     /locus_tag="LNE35_000734"
     CDS             44718..46040
                     /locus_tag="LNE35_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6909914.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            46195..46971
                     /locus_tag="LNE35_000735"
     CDS             46195..46971
                     /locus_tag="LNE35_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK6909915.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            46964..47533
                     /gene="rnmV"
                     /locus_tag="LNE35_000736"
     CDS             46964..47533
                     /gene="rnmV"
                     /locus_tag="LNE35_000736"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBK6909916.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            47526..48410
                     /gene="rsmA"
                     /locus_tag="LNE35_000737"
     CDS             47526..48410
                     /gene="rsmA"
                     /locus_tag="LNE35_000737"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBK6909917.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            48861..49490
                     /locus_tag="LNE35_000738"
     CDS             48861..49490
                     /locus_tag="LNE35_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909918.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(49614..50723)
                     /gene="ugpC"
                     /locus_tag="LNE35_000739"
     CDS             complement(49614..50723)
                     /gene="ugpC"
                     /locus_tag="LNE35_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBK6909919.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            50932..51354
                     /gene="mgsA"
                     /locus_tag="LNE35_000740"
     CDS             50932..51354
                     /gene="mgsA"
                     /locus_tag="LNE35_000740"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBK6909920.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            51677..52213
                     /locus_tag="LNE35_000741"
     CDS             51677..52213
                     /locus_tag="LNE35_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK6909921.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(52253..54016)
                     /locus_tag="LNE35_000742"
     CDS             complement(52253..54016)
                     /locus_tag="LNE35_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909922.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(54019..55734)
                     /locus_tag="LNE35_000743"
     CDS             complement(54019..55734)
                     /locus_tag="LNE35_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909923.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(56513..56737)
                     /locus_tag="LNE35_000744"
     CDS             complement(56513..56737)
                     /locus_tag="LNE35_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909924.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            56928..59705
                     /locus_tag="LNE35_000745"
     CDS             56928..59705
                     /locus_tag="LNE35_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6909925.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            59786..60205
                     /locus_tag="LNE35_000746"
     CDS             59786..60205
                     /locus_tag="LNE35_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBK6909926.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            60217..60687
                     /locus_tag="LNE35_000747"
     CDS             60217..60687
                     /locus_tag="LNE35_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6909927.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            60704..61483
                     /locus_tag="LNE35_000748"
     CDS             60704..61483
                     /locus_tag="LNE35_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6909928.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            61470..62291
                     /locus_tag="LNE35_000749"
     CDS             61470..62291
                     /locus_tag="LNE35_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6909929.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            62308..63357
                     /locus_tag="LNE35_000750"
     CDS             62308..63357
                     /locus_tag="LNE35_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6909930.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            63370..64374
                     /locus_tag="LNE35_000751"
     CDS             63370..64374
                     /locus_tag="LNE35_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6909931.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            65007..66026
                     /locus_tag="LNE35_000752"
     CDS             65007..66026
                     /locus_tag="LNE35_000752"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK6909932.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        22102 a        11476 c        13458 g        19013 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
    17341 gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt
    17401 agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata
    17461 acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt
    17521 atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc
    17581 taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga
    17641 gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca
    17701 gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg
    17761 agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga
    17821 aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg
    17881 agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat
    17941 ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat
    18001 tgtattttat cctttaatta acacaaaaac agctagtata aactaataat gttaattaaa
    18061 agtgtatttt atgaaactat cgttatttat taatgttgca tttttgtaag ttaaaaattg
    18121 gaaatataga ttgaaaaaat gaaagcaata agtaaaataa aatgaatata tgcgaaaaaa
    18181 taatttacat taggaaaaac cttgtaaaaa cagttttttg gcatatttat tttttataaa
    18241 tatttgtaaa ataactatca attttttagt taaaagattg gaaatgagtg ggggaggaat
    18301 tgaagaaagg atttttgaga gatgatatag taggttttat ttttagttaa aataggggag
    18361 atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat
    18421 ataacatcac tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat
    18481 gtcacttcgt tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa
    18541 ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg
    18601 ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa
    18661 aataaaaatg ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa
    18721 aatattaaaa acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac
    18781 gtaaccgata ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca
    18841 gctactgtaa atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa
    18901 acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat
    18961 aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga
    19021 ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga
    19081 ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca
    19141 gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata
    19201 atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg
    19261 gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca
    19321 gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt
    19381 ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga
    19441 gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga
    19501 gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc
    19561 aattggattg attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat
    19621 gatacagagg gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca
    19681 actaaaattt ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga
    19741 gcaagtacaa acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga
    19801 ataacttcag tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg
    19861 tttaaagtgc aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa
    19921 attgaaaacg ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa
    19981 aaaacatggg aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat
    20041 ctattagccg acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc
    20101 tatcgtttca caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg
    20161 gaagacaacg tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat
    20221 acaccggaaa aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt
    20281 ccagagaaga cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt
    20341 gctcatacag gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct
    20401 aaacagataa caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt
    20461 tttgctgtaa tcattggttt attgatagta actatctgtg ctgggatatt cttacaaaaa
    20521 tataaaaaag tttagttaga atataatatt ttttgttaaa tatgactgaa atcaaaagaa
    20581 taaaattccg atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag
    20641 aatcaataaa tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc
    20701 atactagttg ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat
    20761 aaagaaaaag acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat
    20821 aaactggcag atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca
    20881 acgtagtggt attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat
    20941 cgatcacgac ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac
    21001 ctgctggaac gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt
    21061 cttttaattg ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa
    21121 gatagatttg ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta
    21181 gggaaaaagg gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga
    21241 tacccagttt ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc
    21301 accctccttt ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt
    21361 aaattacacg taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt
    21421 tagagagtct ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc
    21481 gattttctga agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct
    21541 agatgaccag ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt
    21601 gattaacgtc ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga
    21661 ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt
    21721 cctcttggca tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat
    21781 ttgattgaag ttatgccgca attattagta ggagcactca caacattgaa attattttta
    21841 tttaccttga ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat
    21901 tttaagccgc tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg
    21961 ttgttgcaac tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt
    22021 ttcgaagcag cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt
    22081 cgtggaggga tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta
    22141 agtccctttc aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct
    22201 tctgtaggga atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt
    22261 ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct
    22321 ttgctaggcg tggcaatcat ctatcttaca ttgactggta ttctaacaat cataatgaaa
    22381 caagttgaag gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa
    22441 ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc
    22501 attccagatg ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt
    22561 ttacgtatct tggctggctt agaaagttct gacagcggag aattttattt agacggtgta
    22621 ccgttcaacc cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac
    22681 ttccagctat ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta
    22741 ttgaaacaga caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg
    22801 attagtgatt tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta
    22861 gcgattgcca gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt
    22921 gctttagatc ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat
    22981 ggggtaaccc aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa
    23041 ttactaaaag tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt
    23101 ttctattcgc tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt
    23161 cagcagataa ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata
    23221 catttgttcc tatgggattt agagataaag acgggaagct cacaggattt gatattgatt
    23281 tagccaaagc agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt
    23341 caatgaagga atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca
    23401 aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag
    23461 tgctgatcac acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat
    23521 taggtgcgca aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa
    23581 aagagatagt caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg
    23641 atctgaaaag cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt
    23701 tgaaacaaag aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg
    23761 attttgcagt tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc
    23821 tagatcaact agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag
    23881 atgttacgcc gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt
    23941 tttccgaata aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct
    24001 cacaacctct tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc caaatttatg
    24061 caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt
    24121 ttcacaacct cctttacata attttatttt gttgtggaag gggtttcgtg ttattctaaa
    24181 agaggaacga ttgaaggtta taggaaagga agagatgaat ggaacacaaa gaacttacct
    24241 catttccaaa agattttctg tggggctctg cttctgcagc gtatcaagta gaaggagctt
    24301 ggcaagaaga tgggaaagga gaatcagtct gggatcgatt tgtacggata cctggaaaga
    24361 catttaaagg aacgaacggt gatttggcag tagaccacta tcatcgttat aaagaagata
    24421 ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct tggacacgga
    24481 tttttcctaa tggacgagga gaagtcaatc aagctgggct cgctttttat gagagattga
    24541 tcgacgaatt gattgaaaac gatattgaac ctatgttgac tttatatcat tgggatcttc
    24601 cacaagcatt gcaagatgaa tataatggtt gggaatcacg tcagatcatt gaagatttca
    24661 ctcattatgc agaaacatta tttgaggcat tccgcgggaa agtccattac tggattagtt
    24721 tgaatgaaca aaatatcttc acttcattag gttatttgtt agcagcacat ccgccaggtg
    24781 tgacagaccc gaaacgaatg tatgaagtta accatattgc taatttagca aatgcatctg
    24841 tcatcaataa atttcacgag ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata
    24901 cgccaaatta tccaattgac agtaatcctg aaaatgttct ggctgctgaa aatgcagaag
    24961 acctgatggc caattattgg atggatgtct atatgtgggg aaaatatccg attgctgcca
    25021 tgaggttctt agaagaaaaa ggatgggcac caacgattga agctggcgat gcagaattgc
    25081 tagaatctgc taaaccagat tttcttggga tcaattacta tcaaacggcg acgaatgcat
    25141 tcaatccatt aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat
    25201 cagaagaaac tggagttcct ggaatgtata aaaaagttga gaatccattt gtcgaacgaa
    25261 caaattggga ctgggagatt gacccagaag gattaaggat cggactgcgt cgtatcacaa
    25321 gccgttaccg catgccggta ctgattacgg aaaatggctt aggagagtat gacaagttga
    25381 cagaagataa acaaatccat gacgactatc gaatcaatta cttacaaagc cacgtcaaag
    25441 ccatcaaaga agcaatttcg gatggagcag aagtcctagg ctattgcacc tggtcttata
    25501 cagatttgct tagctggctg aacggttatc aaaagcgata tggatttgta tatgttgatc
    25561 aagacgaaac acaagaagga tctttggaac gatataagaa agacagttat tactggtacc
    25621 aaaaactgat tgaagaaaat ggagagaacc tttagttcac atacttaaaa caagaaggta
    25681 cggctggata tggccgtgcc ttttcttgat aaaatcattt agaaattaag aaaaactgta
    25741 aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt agttccctta
    25801 cttttttttg tataatgaaa cttgatgaaa tgagggataa taaatggcac aaaagaaacg
    25861 gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca
    25921 aatgctgacg attggtgttg ggtttgcctt gattttattt gctgtcttcg gctttttaaa
    25981 attaggtttt ttaggaatat tgattgcaaa cggctttcgg atcattgcag gaaatactta
    26041 ccaaatcctc tgtcttttat tagccgtatt aggattttgg attgtcatta agaatacaga
    26101 gttttcaatt ggtaaaaacc gacgctggtt tggcggcata ctcttttatt tcggtatctt
    26161 attattgtta catgcccatt tatttggcaa actccatact ggggaaccaa atatcatggg
    26221 gacgacatgg gatttgttag ccagtgatat aaaacaaagt caagtagaca ataacgttgg
    26281 tggtgggatg attggagcaa tcctttatca ttttacttac ttcttgattg cacagcctgg
    26341 aagttattta gcagcagtgc tattactggc aggaggagca ttcctgttca gtaatctgga
    26401 aggctatcag ctgttaaatg gtatccaatc aattggagaa cgagtacagg aattattgga
    26461 aggtgatcct gccaagcaag ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg
    26521 cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc
    26581 agaatatgag aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca
    26641 ggaaccagaa aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga
    26701 aaatatccat cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac
    26761 agcagaaggt ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat
    26821 tgaagcagaa caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat
    26881 accaactgtg gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga
    26941 acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc
    27001 cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt
    27061 aacagatgat atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc
    27121 aggtaaatca ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag
    27181 agaaatcatc gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg
    27241 tgatgtttct ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc
    27301 tggttcgact ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat
    27361 gcgtgctaag ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa
    27421 tatgtacaac ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc
    27481 gcaagcctta caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac
    27541 cggagtaaga aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg
    27601 aacgaaacat ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat
    27661 gatggtagcc agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc
    27721 agcggggatt catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat
    27781 catcaaagca aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg
    27841 gacgatcatt gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt
    27901 gccaatgggt gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt
    27961 agaacgggtc gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat
    28021 gccgacagat gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga
    28081 agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag
    28141 atttcggatc ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt
    28201 tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc
    28261 agaaacgttg ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga
    28321 ataaccaata aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct
    28381 tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga
    28441 tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg
    28501 agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat
    28561 taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat
    28621 ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga
    28681 tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag
    28741 cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg
    28801 tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata
    28861 tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag
    28921 aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac
    28981 gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa
    29041 tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta
    29101 cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag
    29161 tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga
    29221 tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa
    29281 caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc
    29341 atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga
    29401 ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat
    29461 tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg
    29521 gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg
    29581 ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt
    29641 aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt
    29701 ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg
    29761 atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta
    29821 gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa
    29881 ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc
    29941 gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca
    30001 tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa
    30061 ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag
    30121 aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta
    30181 gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta
    30241 tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg
    30301 attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact
    30361 tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata
    30421 attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt
    30481 acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata
    30541 gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa
    30601 gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc
    30661 aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc gacaacctct
    30721 atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct
    30781 ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca
    30841 tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt
    30901 cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga
    30961 gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc
    31021 atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt
    31081 tttgttcctc ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc
    31141 taaaaattaa gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt
    31201 ttaaagcaaa agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg
    31261 gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa
    31321 gtaagataaa taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga
    31381 acatttcgtt ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga
    31441 taatcagtga atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga
    31501 gactgacttg gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga
    31561 atccagaacg aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg
    31621 caatccctca atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc
    31681 gacgaaagaa ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca
    31741 gaggaggaat cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc
    31801 aaacggacct gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac
    31861 atccgtatga attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa
    31921 atggctggga caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc
    31981 aataataaaa agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg
    32041 aaaattactt cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga
    32101 acaccgtgta ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg
    32161 tgaaaatggc tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg
    32221 aacaccgttt attcagataa gggactgtga caagactttt gtcacagttt ctttttgttt
    32281 attctttttt atctttgtat ggcaataata gacaatataa cgaaaattat ttaatggctt
    32341 ttggaagacc atggaaaacc ttgtacaaag ctattttttc tgaaaaattc cttgaaacct
    32401 cacgaaaaaa tcattatttt atgaaaaaga caaaaaaaag tgttgatttt ttaataatta
    32461 ttcattaaac tcttaaacaa cttcagaaaa tattaattat tctgaaaatt ccaataaagg
    32521 ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt gcggtatgcg gccttgtact
    32581 tgctggatgt tatggcggca gttcagccac aagcagtagt tcagcaaaaa gcggttcagc
    32641 agatggagga ggcgtattta acttagtcgt gcctcaagaa atgcctacag ctgacttatc
    32701 agttgcaaca gatacaatca gttttacagc attgaataac gtttatgaag gaatttaccg
    32761 tcttgatgaa gacagcaaac cacagccggc aggagctagc gagttggcag aagtcagtga
    32821 agacggatta acttacaaaa tcaaattacg agaagacgca aaatggtcaa atggtgatcc
    32881 tgttacagca gcggattacg tttatggctg gcaacgaaca gtagacccag ctacagcttc
    32941 tgaatatgct tacctgtatg cgccagttga aaatgcagaa gacattacag ctggaaagaa
    33001 agataaatca gaactaggga tcaaagcagt tggtgactat gaattagaaa tcaaattgac
    33061 gaaacaaaca ccatatttcc aatatctgct tgctttccca tcatttttcc cacaaaaaca
    33121 atccgtagtg gaagaaaatg gggatgccta tgcatctgca agtgataaag ctgtttataa
    33181 tggaccattc acattagaag attttgatgg accaggaaca gatacagaat ggacttataa
    33241 gaaaaatgat cattactggg acaaagatac agtaaaactt tctgaaatca aagtcagtgt
    33301 agtaaaagaa tcttctactg cattgaactt attcaaagat ggccaagcag atgacgtgat
    33361 cttgtctggt gagttagcac agcaaaacgc aaacgatcca gcttatactt ctgttaaaga
    33421 agcgcggaca agctatatcg aattcaatca acgtgaaaag aattcaccat tcaacaatgt
    33481 gaacctaaga aaagctattt cttattctat cgatcgtgaa gctttagtaa aacaagtact
    33541 aggtgacgga tcagttgtct ctacaggatt gatcccatca gatatggtaa aaagtccaac
    33601 aacgaacgaa gactttgcaa aagaagctgg aaaattagtt tcttatgaca aagataaagc
    33661 aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt gattctttag aatttgattt
    33721 gatggcttct gatgatgatt catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca
    33781 agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt ccattctctg tacgtcttga
    33841 ccgctcaact tcgggtgatt ttgacgcagt actaggcgga tgggctgctg actatgcaga
    33901 cccaagcagc tttactgatt tgtttgtcac aggtaattca tacaaccgtg gtcgctggag
    33961 caatgcagaa tatgacgaag cagttaaagc agcttcatca acagacgctg gcgatgaaca
    34021 agcccgttgg gatgatttcc aaaaagcaat caagatcatc tctgatgata tgggtgtcgt
    34081 tccggtgtac caaaaagcag aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca
    34141 ccatgcggca ggcgcttcat gggattacaa atggacatac gttgaagaat aatcgttaat
    34201 accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca
    34261 cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag agcaattagt attccggcaa
    34321 tgatcagcca aaagctgtac ataccgtagc tggcagacga gagcgaggaa aattctggtt
    34381 ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac
    34441 ccgaagaaaa gacccataaa aaaccgccga taatcagaaa cggcaatgac cataagaata
    34501 gatcattaga taattgaatg agtgtaaagg aatggttgat gagatttttt attaaaataa
    34561 ccagaatcag tagaaaagct actgcgtagg ggatagattt ttttttcatt tgattgctcc
    34621 tttatttttg gttcattatc taataatgta gcgaaacatc tgtcagaagt caaaagctag
    34681 ggggaaacat tagtgaatag ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc
    34741 atcacgcttt ggttaattgc aacaatcacc tttttcttga tgcaattgct gccgggcacg
    34801 ccatatacga atcaggaacg tttgagtcct gaaacaatcg agatgctaaa taaacaagtc
    34861 ggtttggata aaccagttat cgtacaatat gggatttatc tttctaattt gcttcaagga
    34921 gattttggaa tttccttcca atttaaaaac cagccagtcg cacatttgtt agctggaaga
    34981 atcggaccat ccttgcagtt agggttacag gcaattatct ttggaacagt cctagggacg
    35041 attttaggaa cgatctctgc catgaagcaa aatacatggg cagatacatc ttctacactc
    35101 gtagcgatat taggtcgttc cattccaaac tttgtatttg ctgttttatt acaatatatc
    35161 tttgcaatca aattacaagt tttgccaatc gcaaaatggg atggctttgt gtacacaatc
    35221 ttaccaacaa ttgcgttggc catgtcgccg cttgctgact ctgctcggtt tatccgtacg
    35281 gaaatggtgg aagtactgca tagtgattat gtggaattag caaaagcaaa aggattgagc
    35341 cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt tgattccatt gatgacttta
    35401 ttaggaccgt tagctgtagc attgatgacc ggatcactgg tggtagaaaa tatctttgct
    35461 attcctggta tcggggaaca attcgttaag tcaatcacaa caaatgatta tcctacgatc
    35521 atggctgtaa cgatccttta ctcatttatg ctgatttttg ttattttgat cgtggatcta
    35581 ttgtacggtc tagttgatcc aagaattcgt gtttcagaag ggagccgagg ataaatggaa
    35641 atgataccaa agcgttatga aacagttgca gatatccctg cggatgaatt tcttccatta
    35701 caaaaaaata ccgaagaaga acgtgaacag attactgcac cttctttgaa ttttatccaa
    35761 gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta tttcaatggt tttactattg
    35821 gtcatcattt tgatctctgt gatcacaatc tttgtttctc ctcatgatcc aactgcccaa
    35881 aacgtggctt atatcaattt gcctcctcgt attccgggaa tcaatatcga tggattgaat
    35941 ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca
    36001 aacgtaaatt tctttttagg aacagacggt ttgggacgtg atgtattaag ccgcttattt
    36061 atgggaatga gaatctcgtt gctgatcgca tttattgctg cattactaga tgtaacgatt
    36121 ggtgtagctt acgggttggt ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa
    36181 cgttttttgg aaatcttatc aggaattcca aacctcgttg tcatgatttt gatgttggtc
    36241 gtttttgaac caggaatttt ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca
    36301 atggcacgta tcgttcgtgc acaaacaatg aaattaaaag atcaagaata tgttttagct
    36361 ggattgacat taggtgaatc aagaatgaaa attgcattca aacatatttt gccgaatatc
    36421 tctagtgtca ttattattca aatgatgttt agtattccat ctgctatttt cttcgaagcc
    36481 tttttaagct ttatcggttt aggtcttacg cctccgtcag cttcattagg aacgatgttg
    36541 agcgaagggt acaagacatt cctgtatctg ccttatctat tatggattcc ggcagtaact
    36601 ttatcggtta tcatgattgg ctttaactta cttgctgatg gattgcgtga tgcctttgat
    36661 cctaaaatga aagagtgaag acgatgacga aaattttaga agtgaaagac ttagaaatct
    36721 cttttgatac gtatgctgga aaggtacgag cgattcgcgg tgtcaatttt catttgaata
    36781 aaggagaaac attggcgatc gttggggaat ctggaagtgg aaaatcagtg acgaccagaa
    36841 gcatcatgcg cttgctgtca agcaacgcca atatagatgc tggtcaaatc ctctttaaag
    36901 gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa aatccgtggt aaagaaatag
    36961 cgatgatttt ccaagatcca atgacctctt tagatccgac gatgcccatt gggaagcaag
    37021 tcgctgagtc ccttagaaag cataataaag tttcaaaaaa agagggacaa aaagctgcat
    37081 tagaattgct taaattagtt ggaatacccg aagctgagaa acgtatcaac agttatccgc
    37141 atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc
    37201 cggaaatcct gattgctgat gaacctacaa cagcgcttga tgtgactatt caagcacaga
    37261 ttttggagct gctgaaagaa attcagacaa aaatcgatac atcaattatc tttatcactc
    37321 atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa
    37381 tcgttgaagt tggaacatcg gaagaaattt tctacaaccc gcaacatccc tatacttggg
    37441 gactgcttgg ttcaatgccg acattagata gtgcgaacga tcgattgtat gctattcctg
    37501 gttctccacc ggatttattg aacccgccaa aaggagacgc attctatcca agaaatgaat
    37561 tcgcgatgaa aatcgatgca gaacaagaac caccattttt tgaactatca aaaacacaca
    37621 aggcagcaac ttggctactt gctccgcagg cacccaaagt gacaccgcca gaagaaatcc
    37681 aacgtcgctg ggctatcttt aaagagaaac aaaaacatac ttacggtcaa ggaggaattg
    37741 ctgatgccaa ataaactact tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa
    37801 aagaatgaag tacatgctgt aaatgacatc agctttcaca tttatgaagg agaaacattc
    37861 ggtttagttg gagaatctgg aagcggaaaa tccactactg gccgtacgat cattcgcttg
    37921 aatgaaccta ctgatggtga gatattattc gacggtcaag atataacgaa gatcaaagat
    37981 aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga tctttcaaga tccatacgct
    38041 tcgttgaatc ctcgtatgaa agtcagagat atcattgccg aaggaattga tgttaatgga
    38101 ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg atctattgag aactgttgga
    38161 ttgaatccaa gccacggtac tcgttatcct catgaatttt caggtgggca acgccagcgt
    38221 attggtattg cacgagcatt ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt
    38281 tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa
    38341 gaacatcaat tgacctatct atttattgca catgatttgt cgatggttaa acatattagt
    38401 gaccgaatcg gtgtaatgca taacggcttg ttattagaga tgggaacaag tgatgaaatc
    38461 tataatcacg gtgttcatcc ttatacagaa agtttgttat ctgctattcc tctaccagat
    38521 ccagaccatg agagaaaacg cagaagaatc aaatatcaac ctgaaccaga tgacggacaa
    38581 gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct atgcaactga gcaggaagtc
    38641 ccatattacg caaaaaaact aaaacgacaa aaggaatctc ttctggttgc taactaggag
    38701 ggacatagat gctaaaagct tttaaattcc gtatgtatcc gacagaagaa caaaaacagc
    38761 aattgattcg tacttttgga tgtgctcgat ttacctataa ccatttgctg aaaaagcggc
    38821 agaaaagttg gcaacaaaca ggagtagcca atttttcgct cacacccgct acattaaaaa
    38881 aagaataccc attcttaaaa gaagtagaca gtcttgcttt agcaaatgca cagcttaatt
    38941 tggatcgggc ttttcgcaat tatttcaagg gacgtgccag cttcccaaag cttaaaacga
    39001 aaaaaagtat gtggcaatct tatacgacca acaaccaaac ccgtacgatc tatctggaaa
    39061 atggctactt aaagttacca aaacaaaaag aactaatcaa gataaatcag catcggccag
    39121 ttgaaggatc gattcgctcc gctacgattt ctgctcgata taatgaagaa ttttatgtcg
    39181 cactactttg tgatgtttcg ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg
    39241 cctatcatcc gaagacattg atccaaacat ctcgacctct agaagtaact ttaccaaaat
    39301 ttcaccagac agaagaaaag ctgcaacatg cacagcggaa attgaatgtg aaagtccgct
    39361 ccgctcacca tagaaagatc aggttagatc aggcaagcaa ttatcagaaa caaaagagaa
    39421 aagtaatgga tctttatctg aaacaaaaga accagcgaga agattatctt gaacaattat
    39481 ctgggaaatt ggtcaaacaa tatgactact tatttgtaga atcttttcca aaagaagagg
    39541 cacatgctga tttttctatt cacgactggc ataaattaat aacaaaattg cgttataagt
    39601 cccaatggta taataaaaaa tttcttttga tcaataccga cggagcagaa gaaagcaatt
    39661 ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca ttcggttatc aagggataaa
    39721 aaatgaatga aactgatagg agcgttaggg ttagccttgt taataaaaag tcttaatgac
    39781 ttttgtttct aggaattttt cttttgaaga aaagctcact aattatgaca aaccaataaa
    39841 acaatagcag aaaaagttcg tacgagcttt ttctgctatt gttaattttt tattcagaaa
    39901 gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc
    39961 gttttttctt gaatgattag gtctgaatct tttgctcaca accttattca tggtgccccg
    40021 gcttgagctg atagattttt gaaggacggc cgattcctac aggacgttca ccgatctcat
    40081 cgaaatattg gagcatagct ttcttaaaat tggaatgatc gatcgtttta taatcgatcc
    40141 ctaagaattt agcaaatact tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt
    40201 gtagaacttg cggctcgtgt tccattttat tgactacacg gttgaatgct ttcaagatga
    40261 tttgactatg gtcaaaagcc aaagtatctt tgccagaaga ctctccagtg actaaatcaa
    40321 gcgagatagc cacttctccg ctagataaat ggatcttgtt actatgacgt tctagtgtga
    40381 accagcgtac ttccttggca tcatctccgg cgattagcgg ttcttctcca ataaaggcta
    40441 agtagctgac tgtgacgacc cagcctcgcg gatctcgatt aggcgtactg aatgtatgca
    40501 gttgctcaat attttctttt gaaataacca caccagtttc ttcctttgtt tctcttaaga
    40561 cgctttctcc agttgactca tccttttgga caaagccacc aggcaatgcc catgaatttc
    40621 gataaggatg cccttttctt tggatcaaca aaactttcag ctgatcttct tctttgttat
    40681 agcacatcag tacgatatcg acagtcaaag agggcttgtc atattctgga cgttcctgtt
    40741 tttgatacca gttaagaaat tcttcttcac ttgctttatg ttcataatat aatttttctt
    40801 cttctttaga agcaaaacgg ttcatctgac cacctccatt gacagtaaaa gtacttttta
    40861 ccttattgta gcagactctg aaaaaaaacc aaggatttac ttgaaaaatc taggttatca
    40921 tttaaatatg aaaaataaat ggaagtatga gattgaataa aaagaaaagc agataaaacg
    40981 gcaatacttt ataccgctct tcacgaatgg caaagaagcc acaaataacg atatatacgt
    41041 caatgataaa acgcaaaatc caagctgctg tcataaaact gttcaaatgc ccatggaggt
    41101 taaaataacc atacatacga atataaaaat agctgaatat taaatgtgct aatgaaagaa
    41161 aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat gattgttcca gtcccttact
    41221 aagatagata gtctttatta taccacgtgt gaggggagaa ataaaaagcc gttttttagt
    41281 agatatgaaa caaaacaaaa acccgcattt attcaaccta ttcatccttt taaaggatag
    41341 attgaacgta gatagaagtc gtttatccag tctttttcat tttgaaaaaa agctaacagc
    41401 atgctttttt tatggtatac tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa
    41461 gagccccgtg gcagaaaagg aaacttttta tatcactacg ccaatctatt atccaagcgg
    41521 caaacttcat atcggcaact cgtatactac gattgcatgt gacgcaattg ctcgttataa
    41581 acggttgatg ggctttgatg tgttttatct cactggagtg gatgaacatg gtcaaaaaat
    41641 cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa tatgtagata aaatggcggc
    41701 tgatgtcaag aaattatgga aaacgcttga tatcagttat gataaattca ttcgcacgac
    41761 agatgattac cataaagcag ctgttcaaaa aatatttgat cgtttattag aacagggaga
    41821 tatttactta ggtgaatatg aaggctggta ctctgtttct gacgaagaat tctttacaga
    41881 aacacaactt gcagaagtgt acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc
    41941 aagtggacac gaagtagaat tggtaaaaga agaatcatac ttcttccgca tgagcaaata
    42001 cgcagaccgt ctattggaat actatgaaga acatcccgaa ttcatccagc cagaatctcg
    42061 taaaaacgaa atgatcaaca actttatcaa accaggatta gaggatcttg cagtttcacg
    42121 aacaactttt acttggggag taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg
    42181 gatcgatgca ttatccaatt atattactgc actaggatat ggctccgaag atgaaagtct
    42241 attcgaaaaa tactggcctg cagatgttca gatggtcggt aaagaaatcg ttcgctttca
    42301 tacgatctat tggccaatca tgctgatggc ccttgatttg cctctaccta aaaaagtatt
    42361 tgggcatggc tggctattga tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt
    42421 ttatcctgaa atgcttgtgg aacgttatgg tttagatgca ctccgttatt atttgcttcg
    42481 tgctgttccg tttgggtcag atggtgtctt tacgccagaa gattttgttt cccgtgtcaa
    42541 ttatgatctg gcaaatgatc tagggaactt attgaatcga acgattgcta tgatcaacaa
    42601 atattgtgat ggactcgttc ctgactatgc ctcacttgtc actccatttg atagtgaact
    42661 gtctacgacg gctgctaacg ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt
    42721 caatacagca ttggctgaaa tctgggtgct gatttctcgt gcaaacaaat acattgatga
    42781 aacagaacca tgggtattag caaaagacga agaaaagaaa gctgaattga atagtgtcat
    42841 gatccattta gctgaatctt tgcgtatcgc agcgatatta cttcagccaa tcatgacaga
    42901 aacgccagag aagatcttca atcaactagg attggactct gaaacaatga atcttgaagg
    42961 attacacttt ggcgaatttc cttcaggaac aaaagtagtt gcaaaaggta caccaatctt
    43021 cccacgttta gatatggaag aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac
    43081 tcaaacaaac gaagatacag tcaaatggga tccagaagaa acagaacttg tttcaacgaa
    43141 agaaaaacaa atcaaatttg atgtatttga aaaagtagaa ctaaaagtag cagaagtcat
    43201 caactgtcaa aaagtagaag gggcagataa gttgctgcaa ttccgactag atgctggaga
    43261 cagccaagac cgtcaaatct tgtcaggaat cgctgaattt tatcctgatc caagcgaatt
    43321 gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat
    43381 cagtcaagga atgatccttt ctgcagaagc accagatggc tctttacaag taatcgaagc
    43441 accaaaatcc atgccaaatg gctcagaaat cgcatagaaa ttcaaagggg gtatgacact
    43501 agttgtgtca tacccccttt ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta
    43561 ttacttgaag ccaatcatat aatacagaaa cagagaacta tccaaaataa atcctagaaa
    43621 aagaagtacg agcttcgctg atttttgtta atttagaata atactaatag gaatcgtaaa
    43681 aacagaaaaa aagcataatg ataaagggtc gggcagattt taattgattc atctggtact
    43741 cttttttatt gtccttacat tcataaagtt tgaatgcaat gactttatta catctatatt
    43801 tatatctaac agtagcgttg atgtgttaat tataagttct atcactttat gttcaattag
    43861 tttgtgctct tatatgtgtg attgaaaata tagataaaaa aacaatcatc ggctttgact
    43921 tcgatgattg ttttctttat tttgtaaaaa tcctatcact tgattttatg gtaacctttt
    43981 atttttgtct tcaaacggct tggtaagaga gcagtaagca gtgcaaatgg gatcgaaaag
    44041 acataccaga aaatcgtacg tccgatacca taatgttgat agagctttgt caacatcacg
    44101 tgtaacaaga tcaaccctaa aagcaaggag ccagctataa accaatgatt atcccaatgc
    44161 atgacttcac taagtgccat cactccaaaa ggatagaaat aaaaatagat cccgactaag
    44221 gtcatccgcc agttctttgt aaatgttccg atattcaata aaaagaaaat cgctgaaagc
    44281 aaaataccga taggcggaaa aagcccaacc aaaaaggaat acaacatccc ccacaagatc
    44341 atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca aagtcagcaa tattagtttt
    44401 tgtgataacg ttgcaacagt cattaatcca aataaaatta atgtgaacca atgccaatgg
    44461 gactgctgtt gttctttacg ggaaaaatcg acccggttgg ataaataatc tgctaatttc
    44521 ggataagaac gcttaaaatc cataaactgt tcctccttcg tggaaaaggt aggtttagta
    44581 tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga gtttttcact gaaagtggag
    44641 aaaaaagaat ggtaaattaa aatattctca gttatactga aaattatacc aaatttcaaa
    44701 gaagaaagag gatgggaatg aaaggcaaac taaaacgaga aatcaatctg tttggcgcat
    44761 tagctaccgt tatgggaacc gtgatcggtg caggcgtctt cttcaaaact gctgctgtta
    44821 ctgctagtac tcagtctaca agtttgacat tgctagcttg gcttttgggt ggtttcttaa
    44881 cgatctgtgc tggattgaca gtggctgaat tagctacagc aatcccggaa actggtggag
    44941 ctgtaaaata tattgaagct gcgtatggaa aattgcccag ctttttatta ggatgggcgc
    45001 aaagcttgat ttactttcct gctaatattg ctgcattatc gattatcttt gcgacacaat
    45061 tgacgaactt attacaatta tctaccgatt atcttttgtt gattgcagtg atcaccgctg
    45121 tttctgtgac agggttgaat ttgttaggga caaaagtcgg tgcttctgta caatcagtga
    45181 cgttgattgt taaattaata ccaattgctg tgattgtcat atggggatta ttgaccccag
    45241 gacaaggaac tgtccagtta tttccaatcg aagctggaaa agatgttact tttgtcgaag
    45301 gattgagcag tgcgttgtta gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg
    45361 caatggcagg ggaaatgaaa cggccggaaa aagatttgcc taaagcaatt attcttggtt
    45421 tgagttttgt aacagtcgtt tatctattga tcaattttgt ttttttgaaa actttgccga
    45481 ttgatcatct tgccggtaat ttgaatgcag cttctgaagc atcagacgtc atttttggag
    45541 gaatcggtgg aaagctggtc actattggga tcttgatctc ggtatacggt gcattgaacg
    45601 gttatacact aactgggatc cgcgtgcctt atgcgatggc tttagaagac gagctacctt
    45661 tcagtaaaca gctgacaaag ctttcaaaga agtttacggt tccttatgtt cctgcggttt
    45721 tccagttagc agtagcttgt attatgatga gccttggttc tttcgacttt ttaacagaca
    45781 tgttgatttt tgtgatgtgg ctattcagtt tactgatttt tatcggtgtc tttattttaa
    45841 gaaaaaaagc gccggaattg ccacgtccat ataaagtacc actttatcca atcgttccga
    45901 ttattgcgat tctcggtgct attttcattt taggtatgac gatgatgacg caaacgaaat
    45961 tagcattgat cggtataggt gttaccttga taggtatccc agtctattat caaaaaaaga
    46021 aaaaacgctc agaagagtaa aatatttcaa caacagacag aaaaagtacg aactaggtcc
    46081 tctagatctt cgtgcttttt ctatttcaat aaaacaagtt cttctatttt gatttttgct
    46141 acttctgcaa gtagtgctta tgtgctaaag taagaatgaa aaggagagat aaacatgata
    46201 tttgattctc atacccattt aaatgctgaa cagttcaatg aagatattcc agaaacgatt
    46261 cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg
    46321 attgaaaagt ctttgcaatt aagccatgac tatccaaata tatacagtat tatcggctgg
    46381 catccgacag aggctggcag ttatacgaaa gaaatcgaaa ataaattaca agaacaattg
    46441 actttaccaa aggttgtagc attaggggaa attggcttgg attatcattg gatggaagac
    46501 ccgaaagaag tccaagataa agtatttcgc agacagattg ctatcgcaaa agaaatgaac
    46561 ctaccaatca gtattcatac tagagaagca cttgaagaca cgtatcaaat cctaaaagaa
    46621 gaagatgttc gagatatcgg tggaatcatg catagtttca gtggtgatta tgaatgggca
    46681 aaacgatttc ttgacttagg gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa
    46741 gcattagacg tccaagaggc tgcagcaaat gtaccaatcg aacgattact tgtggaaaca
    46801 gatgcccctt atctggcacc agttcctttt agaggaaagc gaaatgaacc aggctatact
    46861 cgttatacag tagaaaagat tgctgaactt cggaatcttc cgacagaaga agtggctgcc
    46921 caaacatgga caaatgcgca ccgattattt ggtctaagcg aacatggata agatgaagat
    46981 ccaagagatt atagtcgttg aaggaaaaga cgatacaaga aggctgcggg aagtagttga
    47041 tgcagatacg atagaaacaa tagggtctgc aatcaatgaa gagatcctga tgcaaattga
    47101 acatgcccaa gagactcgtg gagtgatcgt ttttaccgat cctgattttt caggagaaaa
    47161 aataagaaaa atcattatgg aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac
    47221 acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag catgcaagtg atgctgctat
    47281 tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc caaacggctg tttctgaaat
    47341 cactagacaa gaccttttag actatggttt aatagctggg gcacaagcaa aagaaaaacg
    47401 agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg
    47461 tttgaagatg tttcgtatta cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga
    47521 acaacatgag tgaatatcgt gaaattgcga caccctctag aacaaaagag attttattga
    47581 aacatggttt ttcatttaaa aaaagtttag gacaaaattt tctgactgaa ccaaatattt
    47641 taaggaaaat cgtagaaact gcgcaaatag atgaacatac gaacgtgatc gaagttggac
    47701 caggaatcgg tgcattgacg gaacaattag ccaagcatgc aaaacaagtc gtagcatttg
    47761 agatcgatga tcgtttgatc cctgtattgg ctgatacgat gcaaccctat gacaatgtga
    47821 ctattgtaca tcaagatatt ttaaagaccg atttgtcaac agctgttaga gaaacatttc
    47881 atgaggagct tccgttaaaa gtcgtagcga atttacctta ctatattacg acaccgatca
    47941 tgatgcattt tcttgaatca gatttggttg tggatgaact agtcgtcatg atgcaaaaag
    48001 aagtagctga tcgtatctct gctgaaccag gaacgaaagc ttatggttca ttatcgattg
    48061 ctgttcaata ttatatggaa gccagtttag cattcatcgt accaaaaaca gtatttgtcc
    48121 ctcagccaaa tgtagattca gcgattttga aactgacaag aagagatata ccggcagtcg
    48181 aagtgacaga cgaaaaagca tttttccgat tgacaaaagc ggcatttcag caaagaagaa
    48241 aaacgctttg gaataacttg cagcattcgt atggcaaaga tgaccagaca aaagcatggt
    48301 tagcaaaaag cttggaaaca gctggaatcg atccaaaacg tcgtggggag actttgtcat
    48361 tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt
    48421 tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caaacaaaaa
    48481 tagttcctca tgtttttcgt ttgtttgggc ttgttaccgc agatgcaagc ttttgaacac
    48541 cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa gtcacaagga acaatttcaa
    48601 aaaacagctt ctcatgtttt tgagttagtt ggtcttatta cttgatgcaa gcttttgacc
    48661 actattatgc cgaacaagga gatgtgacac aaattttgtc acatctccta tttttgtttc
    48721 ttgatcaaaa aattcttata cttgatactc acactcattt tgtatggatt tagaaaagaa
    48781 tatgtgtctt gacagttatt agtaagtgag ttatactaag tcgacattta tgtgtcaaga
    48841 catattagga gagagaaaaa atgagaaaaa attcagttcg aaaactaacg atttctgctt
    48901 tattgattgg gatgggggtc attatcccaa tggtcatgcc taaaatcatg attccaccag
    48961 cttcctttac tttggcgtct catgtgccat tatttattgc gatgtttttt acaccaggag
    49021 ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt tttaacaacg ccggttatca
    49081 tcgcattgcg agcattgtca catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa
    49141 agcatccgag tatcgtttta aaaaatggac agtttacttt tgctaattgg cgtttccaag
    49201 ggttcaattt tgtgatcggt gtgattcatt ctttagcaga aatgctagta gtcagcatct
    49261 tttactttat tggtaatatg cctgaaacat attattctca aggatatttt tatacagtat
    49321 tcatcttgat ggggattggt gggcttatcc acagtttagt tgattataat attgcttatt
    49381 ttatagcagg aacactcagc aaatattttg atatccccgt atttacggca gcaaaaaaag
    49441 aaaatcggaa agagtcgatt caagggtacg cgaagaaaac gacatattag acatgatctc
    49501 tcaaaaaaaa aactctcatc tagtaatgga taataaatcc tctaagatga gagttttttg
    49561 tcctaaaaag agctgcacca acatcttctg gcacagctct aacagggggc aatttatgga
    49621 agtttgatga cttgttctgt ttctttatca aagaagtggc ctttgctgat gttgaaggca
    49681 aggtcgacta tttcgcctgg tttatggaaa tctcttgcat ctacttttga gatgaactca
    49741 gtagaaccag tttttgtgta aagcatcgtt tcagctccaa gtaattcgga aacgacaact
    49801 tctgatttca ctacggcttc aggtgaagct tcaagggcta cttgttcact gtggatgtct
    49861 tctggacgaa taccgaagat aacttctttt ccttcataac ctttttcaac taatactttg
    49921 ttttttcctt caggaatacg aagcttaagc ccatgtccat ctgtgataac accatttttc
    49981 aatgtcacat taaagaagtt catggcagga gatccaataa agccggcaac gaacatattg
    50041 acgggtgtat cataaacttc tttcggtgta ccgatttgtt ggataaatcc gtctttcatg
    50101 ataacgattc ggtcagccat agtcattgct tctgtctgat cgtgagtaac atagatggtc
    50161 gttgtttcaa gacgctggtg aagtttggca atctcagcac gcatggctac acgtaattta
    50221 gcatctaaat ttgataatgg ctcatccatt aagaagactt ttgcatcccg aacgattgcc
    50281 cgtcccaatg ccacacgttg gcgttgtcca ccagataaag cagctggttt tcgtttcaag
    50341 tattctgtca gacctaagat ctctgctgca ttgtccacac gttttttgat gtctgcttta
    50401 tcatatttac gtaatttcaa gccaaaagcc atattatcaa aaacagtcat atgaggatac
    50461 aatgcgtagt tttggaaaac catggcaata tcacggtctt taggtgccac gtcattcatg
    50521 acttttccgc cgatatttag ttctccttca gagatatctt ccagtcccgc gatcattcgc
    50581 aaagtagttg actttccaca accggaagga ccaacaaaaa cgataaattc acggtctttg
    50641 attgatagat taaaatcagt tacagaataa ttttctgcat tgtcatattt tttatatacg
    50701 tttttcagag ccatttctac cataatacgt tccacacttt cttttttagt tttattaact
    50761 atttaaagta taaatgaaag cgttaactaa cttcaatgtc atgttgaaca agaatttttt
    50821 cttttttttg tcaatgtgct agttttaagc tttaataaat atccataaga aataaaaact
    50881 gatacactgg agtaaataag tatgttaaca atatcaaaag gagtgtctct tatgaaaatc
    50941 gctttgatcg ctcatgaccg aaaaaaacct atgatggtga aattagcaac agcgtataaa
    51001 acaatattgg aaaaacatga actttttgct accgggacga ctgggcagag gatcacggaa
    51061 gcaaccggct tgccgatcca tcggtttaaa tccggaccgt taggcgggga tcagcaaatt
    51121 ggagccatga tttcggagga tcagttagat ttggtgattt ttcttcgaga cccgctggct
    51181 gcacagcccc atgaaccaga tgtaacagca cttatccgtt tatctgatgt ctatgagatt
    51241 ccgttagcta cgaatatcgg aacagctgag gtgctactaa gaggactgga agccggcttc
    51301 ctagattttc gaagaatcgt ccatgaaata gacaagcatc cactttcatt ttgatggata
    51361 agcgtatgga aaaataacgt ttaatgttcg cctatatgta gctataaaag ctaaacaaac
    51421 tgataatatg cgatataata cgaacgtatt ggataatata aacaataata ttagtttttc
    51481 gtatttgcag taaatgtgaa aatatgctat agtttctgtg ggaaaagatc ccaaaaataa
    51541 tacagagtag aaaataaagc gttaagtgct aagaggatgg gatgttgcct cttggacgaa
    51601 ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat gatacgatgt agcagctctt
    51661 aaaggagatg ctaaaaatga agaaacaccg gttagacgca cgtatgattg ctattatggg
    51721 cttattgatt gcattgatgg tcactttgtc ccgactggta gcaatcgaga ccccatttat
    51781 taaaatcagt gtcacattta ttccacaagt gatcatgggc attctttttg gtcctttttg
    51841 gtcagggata ggcgcagttt tagctgattt agtaggaatg gccctcttct cgaaatctgc
    51901 attctttatt ggatttacat tgaatgcatt tattgaagga gcaatctacg gattcttttt
    51961 ctatcgaaaa gagatcactt ggaaaaatgc cattctggct acgcttagtg tcacgctgat
    52021 catcaactta tttttgacac cattatggct tgcgttgatg taccatgtgc cattgttcag
    52081 ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata tggttaccga ttcaatcaat
    52141 tgccatttat tacgtaggac gtagtattcc ttacaaaaaa attttaagaa gtttagcgat
    52201 ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa gactgcttgt ttttattgtg
    52261 ctgtgaattg tgcttgatag agccgtcggt aataaccatc tttttgaagc agctcatcat
    52321 gggtgcccat ttcgacaata gctccctgat ccatcactaa tatctggtcc gcatttttga
    52381 ttgtggacag gcgatgggca atcacaaaac tggtttttcc ttccatcatt gctaagaagg
    52441 cttgttggat ttttttctct gtcaatgtat cgactgagct ggtagcttca tctaagatca
    52501 acataggggg attactgatc atcgttcgag caatcgtcaa cagttggcgt tgtccatctg
    52561 atatctttaa cccttgaccg ccaatcttag tatctaattt ttccggcaaa cgttctacaa
    52621 attcatacat ataagaggct tttagggcat cattgatttc tgcgtcactt gcttccggat
    52681 tcccaaaacg aagattttct cgcaatgtac tgtcaaatag ccaagtatct tgtaacacca
    52741 ttccaaaact tttacgtaat gtatcacgtt ggatagagcg gatgtcatgg tcatcgactg
    52801 taattgttcc ttttgttgtg tcataaaaac gcatcagcaa attaacaagt gtggattttc
    52861 cggcccctgt ttttcctact atagcaatgg tttgacctgg ataagcagta aaattgaaat
    52921 cttcgatcaa acgctgcttg gggtcataag aaaaagaaac atgatcaaaa gagacttttc
    52981 ctttgatatt tttgggttcc aaggcatagg catctggcaa atcaggcttt tcttctggct
    53041 gatcgatcaa atcaaatgct cgttctagac cagaaaaagc ggtttgcagt tgtgtcgtga
    53101 ttccagaaag ttcgataaat ggtttagaaa actgacttga gtagatcgta aagctggaaa
    53161 tcacaccaac agtgattgac gaaccactct tcaatgccaa taggccgcca actaaaccaa
    53221 tcgataaata agcaaggtga tctacaaaac gagaagacgg gtttgtcaag gaagaagaaa
    53281 attgtgcccg ttgtcctttt tcatatagat cagcatttag cagttcaaat tgctgttgac
    53341 tagcttcttc ttgctgaaaa gctttcacga ttttctggtt accgattcgt tcggtcacaa
    53401 agcccgagat atccccaata atcctttgct gtctatcgaa atttgtttgt gaagcacgag
    53461 caacgatcca actcacaagg aaaataattg gtgtagaaca aagaacgacc aatgtcagaa
    53521 gtggacttag acgcatcata aaaataaacg aaagaatgat gattgccact ccagaaaata
    53581 attgattgaa ggccgcagaa attgcaatgg aaatattgtc catatcattt gtaaatcgac
    53641 tgacgatatt tccgtgggag ttttgatcat aataacgaag tggcaatcga ttcagatgag
    53701 caaatgcatc ttttcttaat tttgcaacgg ataagtaagc taaccgattg cttaacactt
    53761 ggatgagcca ttgactgact acgttgatta aaacgatacc accgaataga ataagtaaat
    53821 ggaacagtcg attgaagcgg acctgtcctt ctccaatcat ttgatcgata ctttgaccaa
    53881 tttgtaacgt catcacaact gttgcaatcc cattgatgat tcctagtatc acagctagcc
    53941 aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg gcggagcgtc tgcaaggata
    54001 atttttttac tttcataact attcttcctc ctgtgatgcg acgatttcct gatattctgc
    54061 tgattgagca agtaattctt catgggtacc ctgagcagca atcttacctt gatccatcac
    54121 taagatatga tgtgcttctt gaatagagcg gacacgttgg gagataataa tcactgtact
    54181 gccaagataa gtctgtagcg ccttacgcaa atccaagtct gtttgataat ccaacgcact
    54241 taaagagtca tctaaaatca gtaatgctgg cttggcaatc aaagctcttg caatcgtcag
    54301 tcgctgtttt tgtccgccag agaaattttt cccattttcc atgacttttg tatctaaacc
    54361 atttggaagc tgacggacaa attcttctgc ctgagcgatt ttcagagctt cccaacattc
    54421 tttttctgtg gcgtctttct taccccattg aaggttttca cgaatagtcc cggtaaatag
    54481 tacagaagtt tggggaacaa gagcaatttg ctgacggaga tgatccagct gccaagaacg
    54541 aacatccatt ccgtccatta taactgtacc ggaactgaca tcatagaaac gcggaatcaa
    54601 ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg attccaagga ctgtatttcg
    54661 cttcagctga aatgtaatat tttggagaga aagaccagaa tcaggagtat aacggaagtc
    54721 aacatggtca aaagcgataa ttgcttcaga agaaaaatcg ggagtagtac tgacctcagc
    54781 ttcaagaatc gaattttctg tgtctagcac ctctttcacc cgatttcctg aagcttcggc
    54841 acgtgtgaag atcacgacga ggtttgaaac aacgatcagt gccaaaagca tttgattcat
    54901 ataattgatc aaagcaagaa cttgtccttg ttgcaagcta ccaatattta ctttgatccc
    54961 gccaactgtc agcaaacaga tgatcccgac attcatgatc aaggttgtag caggtgataa
    55021 tagggcagag atattggaaa cacgtaaata attgttcgct agatcatttg tgcttttatc
    55081 gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg atgacacgaa caccgctaag
    55141 attctgacta acgtgttcat tgagctgatc tagtttttct tgcacttttt ggtagagagg
    55201 aacggttttt ttgatgataa aaaataaaat gattgagaaa ataggcaata atgctaagaa
    55261 aaacagacct acttcccagt cgatcacaaa agccatgacc actgacccga tacttaaaaa
    55321 cggtgctctg atcaataaac gaatgaccat tgctaaagct agctgaagct gattgatgtc
    55381 gtttgtcatt cgggtgatca aggtatctgt tccaaaatga ttcaactcgt tgtgagagaa
    55441 agtattgatt tttttgatta gctgattacg taactctgta ccaaatcctt gtgaagctac
    55501 tgaagcatag tattggcaaa tgatagcaca aatcagacca atcacagaca tagcgaccat
    55561 ccataagccc atttcaatga tttttcctcg gtcattcatt tttagtccat tatcgatcaa
    55621 ggaagccatc attaaaggca ggacaagttc gaagacagct tcaagaaatt tgaagaacgg
    55681 gcccaaaatg atttgcttac gatagtcttt tgcataacgt aataactgaa tcatatgatt
    55741 cctcctatta tttatttgtc cacaattgaa aaaacaagta ctaccacatt gtagcttagg
    55801 aaatttagaa tgaaaagaga acaagccttt tcataaatga aagttttatt atgaagaagc
    55861 tttagaaatt gaatagacta ttttttttaa aaaaaagaaa atgagaaaac atgccagtct
    55921 cttcattcat aggaacgtct attttactta atttttctat aaatgaattc tgcataaaaa
    55981 atatatcaag taaatgttta tagatgaaac tctatttact tcttttttaa tttgttcttg
    56041 caatcttttt tgctacgtaa tttttgtaaa aaagtgggtt gagagaaatc taattgattt
    56101 tcaaagaagg agtatgaaaa tgagaaaaat atttattcgt ttattgtggg tactatctta
    56161 gaggaataaa taatagtgga gaaaactatg gtttggcgca gattctatca ggtttgatgg
    56221 aacgaagaca acctcccgac atcaagtcaa aagctacttg agattttgat ctgaaaataa
    56281 aaaaagaaaa caaggtagga acatcaatgt ttctaccttg ttttcgtaat caatgggaaa
    56341 aaactaagag tggtgtacgt aatcaataaa aaggatggtg acaacagtac ctctttactc
    56401 tctgaaagca tcataactcg tttttcctca ttatgccaat aaattgttgt tataccacat
    56461 ataaggattt gtaatagcta aaaacagaca ggaaagttga aatctgtgaa tcttattttg
    56521 tatggaagga tttatagaac gcaaaaatca agataaagat gaataaaaat cctgtttcgg
    56581 ctatatattg cgtatctacg ccaataaaac gagtttgttc tgtcatagcc atataaagca
    56641 aaggcaaaaa tacaactaca gcgagataag atccgatgat ttctcgaaaa gttttccagg
    56701 aagtatgttt cctgttttta tatttatccg atttcattga atagtcatag cctccttatt
    56761 ctttcatatg ttgaaattcc agtataaaaa ctggaaggaa ctgtacgttt tttagaataa
    56821 aaattataag ggataagtag gctgctgtct ataataagat atatttttca attctttatt
    56881 aaagatttta ttgacaaaat gaatccgttt tctatgatgg aggaggggtg aataagttgt
    56941 tgaagaagga aattttatgt tacttagaga accagacagc ctttttcgat ttagaacata
    57001 tgaacgaggt ctttacagca caaaggttag ctgatcattt tggggtaaaa agaaacacag
    57061 tcagtcatta tttgaaccag ttaaatgatg agcaactact agtcaaaatc aattctcgac
    57121 cagtgattta tttccacaaa gaagcttttg aaaaacaatt ttttaagtta cgcacgaatt
    57181 tttatttttc tatcgatgat ctcaaagagg aacagccttt tttcgcgcaa ccaaaagact
    57241 tattctcttt tatgatcgga tacgacgcca gtttgaaaga aagtatcgaa caaatcaaaa
    57301 cagctcttta ctatccagat ggcggattac cacttttgat tacaggcgaa agtggcacag
    57361 gtaaaagtta tttagttaat ttggtctatc agtattgtct gcttcatgat ctgctagaag
    57421 attctgcacc attcatcact tttaattgtg cacaatatgc agataatcct gaacttttga
    57481 ctagcaattt gtttggacat gttaagggag cgtacactgg tgcagaagaa aataaaaaag
    57541 gggcttttga agcagcagat ggtgggatct tgtttttaga cgaggtacat cgcttaacac
    57601 cagaaggtca ggaaaaactg ttcacctatc ttgatcaagg tgtgatttat cgtatgggag
    57661 aacctaattt gtcacgccgg atcaaaacac gtctcttttt tgctacgact gaagagatta
    57721 ctagtcattt cttaacaact ttcattcgaa gaatacctat ccgaatcgaa ctacctgcac
    57781 ttaatcagcg tagtcgtaat gaacggctag aactgattta ctcttttttt ctcgaagaac
    57841 aaagaaaaat ggggagccct ttgtcagtca gtggaccagc tttgtcactt ttaacaagtc
    57901 agaaattatc cggaaacata ggagagctga aaaatatcgt caaagtttca gcagcaaaag
    57961 catatgctga acagcgtgaa cagcctgaaa tccatgtcac gatccatcat ttgcaaaaag
    58021 aactacttgc gcagcctatt actaaaaata cagcacagca aggaatctat attacgcaag
    58081 accagacctt agagcaatta atggagaagc aacagcccga acagcagcga atcgttaaaa
    58141 gttttgagca aatcctaatt gatttcaaga aaaataactg tctgctatat tcttgcgaag
    58201 gaaaattaaa gcaagaagtg attcaattat ttgatttttt gctgtttgag accagtagac
    58261 aagaaaaaca cgagctgctg gtttattgga cacaatctat ccgagagacc tttcgacaaa
    58321 tggaaactgc ctatcaaatc aaattcgatg gaaacagtgt ctatgccttg agttactatc
    58381 tctatcagcg aagcactatc aaatggttac cggaagaaca tgatattatc caactaatca
    58441 aagaattaga acaacaagtt tctcagtctt atccatctag ttatcactat gtccaacgaa
    58501 ttctggaatt aagtaagatc aaattagata ttgaagacac aagtatggat cggattttac
    58561 tgacgatcta tctaaaaaaa gcgaaatggt caaaagaaaa acgactgcca aaagcgatga
    58621 ttgctgctca cggttatgca actgctagca gtatggcaaa tgtagtaaat cgtttattac
    58681 aagaggatct atttgaatca tttgatatgc cactagatgt cacaccacag cagattgctt
    58741 cagaaatcct tgattattgc gaaaacagcg atgtttcaaa tggattgatc atattagtag
    58801 acatgggatc tctgaaagaa atccatcagt tttttaaaaa acagttatcc gtaccgcttt
    58861 tgattttgaa taatgtgacg acacctttag caatcacagt aggggaatgc ctgcaaaaaa
    58921 atgtatcatt agaagagata gcagaagaga ctgttcgaca gatccagcca gaatggcgac
    58981 tgctctatcc agaggaaaac aaacccaaag cactcattac tacttgcttc actggaatcg
    59041 gtacggcggt ccacttaagt gaactgctag aaaaaagctt acctacggct tgtcagttga
    59101 aaatcatccc atatgaatat cagcaattaa aagataagaa aaacagtgac ccattatttt
    59161 ccatatacga agtcgtagga atgattggca ccacagatcc tgatatcacc aatatcgact
    59221 atctttcatt agaagacctc atctcggggg aaaagatgtc tgtactagct gaatggttgg
    59281 acactactat gaatgcttca gagaaggaaa tattcaatca gcgcatcatt cgtaattttt
    59341 cattggaaaa agtactggat tccgtgacta ttctagatac agagaaggtg atgggagaaa
    59401 tcgatcattt tatgagagaa ttagaggtgc agttgaatcg aagcatcagc aatcccaaaa
    59461 aattagcgtt gtatgtccat gtgagctgtc tcattgaacg attgatccga caaatgccaa
    59521 tcgaaaccta tcaaggatta gagaaatgga agcagtgtca aaaagaaggg ttatctgcta
    59581 ttaaatctgc gtttagtgtc attgaagagc attatagtgt catgatcccc aattctgaga
    59641 ttgcctatat ctatgatatt ttgagtgaag atacagagtt tctctcgata gaagaagaat
    59701 tttgaacata agcaagttgt tgccactaat tttggcatgc aacttgcttt tttagtatgt
    59761 gtaagtgaaa agaggagatg aaaaagtgga tagagaaatt atcttagctt cccatggaaa
    59821 atttgcttca gggatattag attcattaga gctgatctat gggaaaaatc attctattac
    59881 tgtattcgat tgctatactg atgagaattt tgatttagcg gaaacagtaa ggcagctttt
    59941 tatgaggtac aaagataaag aaatcattgt tctgacggat ctttttggcg gaagtgtcaa
    60001 caacgaattc ttacaatata tcaatcggga tcatgtctat ctcgttgccg gattgaattt
    60061 gccactattg attgagctag tatctcggtt ggacgctgaa gtagagacaa ctgttttgat
    60121 ccagcaggtg ctaactgatt caaaaaaaat gatccagttt tgtaacgagc ggctcaatca
    60181 gaaaatagaa gaagacgaat tttaggagga agaaaaatga ttgtattgac aagagtcgac
    60241 catcggttat tacacggaca agtagctttt tcatggacac aaacaatagg cgcagactgt
    60301 attttgattg ctaatgatga tgtacctacg aatgagatac gaaagacaac gatcaaatta
    60361 gccaagccac aaggagtcaa actagtgatc aaatcaattg atgactcgat tgccgcgttg
    60421 aaaagcgggg tgacagataa atataagtta ttcatcgtag tggaatcgat cgaggatgca
    60481 tataagctgg cagaagcgta tccagaaatc aaaaccatca acttaggagg gatcaaggca
    60541 aaacctggta ctcgcagcat cggaaaagcg gtaaatattt taccagaaga ggaggaacgc
    60601 ttgaaacgat taattgacaa acaaatagaa gtagaaatcc gtcaagtacc agcagataaa
    60661 aaagtggatg taagagaagt tttgtaagaa aggaagaacc tcaatgattg tacaagcagt
    60721 cttattagga attgttgcat ttgtcgcaca atcagaatat gcactaggga catcgttgct
    60781 ttctcgtccg attgtgacgg ggctgtttac aggaatcgtg ttaggtgatg taaaaactgg
    60841 gatcattatg ggcgcaactt tagagttagc atttatcgga tcgttctcag taggtgcttc
    60901 gattccacct gatgttgtga ccggtgggat tttagggacg gcttttgcta ttacagctgg
    60961 agccggtaca gaaaccgcgc ttttactagg tttgccgatt gctacgttga cattgatatt
    61021 gaaaaatatt tacttagggc ttttgattcc aattatgaac cataaagcag acaattatgc
    61081 agaagaagga aattataaag gagtcgagca tatgcacttg cttgccggta tcggtctttc
    61141 cttgatgctt ggtgttgtgg taatggtttc ttatatggtg ggaagcaata cgatcggaaa
    61201 tctattaaat atgattcctg acttcgttca aaaggggttg tcagtagcta cagggttgat
    61261 tccagctctt ggttttgcga tgcttgctcg tttgctgatc aacaaacaag ttgcaccata
    61321 ttttttctta ggttttgcta tagcttctta cttagagatt cctgtgaccg gagtagctat
    61381 ttttggtgcg attttagcag ttgtggtagt caatattatg aatgtccgtc aactaccact
    61441 tcaaacaact gcaggggagg tagaagaaga tgaagaattc tgagatcaaa aacagcaaaa
    61501 taacgaaaaa agagctaaat cacgtatttt ggcgttcttt tcaaatggag ttttcttgga
    61561 attatgaacg tcagatgaat atggcttatg tttatgcaat gattccgatt ttgaaaaagt
    61621 tatatcagtc gaaagaagaa atggcagcag ctttgaaacg gcatctagag ttttttaaca
    61681 cgaccccaca tatcgttaca ctgattttag gaatcacggc tgcaatggag gaagaaaata
    61741 aggatgatcc tgaatttgat gtgactgcga tcgatagtat caaaacatct ttgatgggac
    61801 ctcttgcagg gttaggagat tctttctttt ggggaacttt gcgattgatt gccacaggtg
    61861 tgggtacctc tttagccatg caaggaaata ttttaggccc tatcttgttc attttgattt
    61921 tcaatattcc gcacattctg tttcgctatc tagcaacagg ttggggatat cgcttaggaa
    61981 cgggtttttt gaaaaagatc caggcaaacg gtatgatgga aagtttgaca ttaggcgcgt
    62041 ctattattgg actgatggtc gttggagcaa tgactgccac gatgatcacc atcaatattc
    62101 ctttaaaaat cggttcagga gaaaatgctg tgactgttca aagtattttt gatgatatcg
    62161 ttccaaatat cttatgtctt ggagcatttg gcatagtgtt ttatctattg aagaaagaag
    62221 tgaaaccatt aacgatcttg atcggactag ctttctttgg aatttttggt tcgtggatag
    62281 ggatttttta aaaaaggagc agagaaaatg tttacgatgc aagattatat ctatgaagaa
    62341 aaggaagtac taagtacgat cttgaaaaag aatgattttt cgacaagaga acatatgaaa
    62401 aaaacagtga atttactgat cttagcaacg ggttcatctt ataacgcttg tttagcagcg
    62461 aagccagcat tggaatctta tggtgatctt actgtcgata ttcaagaacc tttttatttt
    62521 ttcaattatg gaaaactttc tccttcaatc gatactgtga ttgctgtgtc acaaagcggg
    62581 aaaagtgctt ctactgttga agcagtaaaa atgatacaaa aacaagggtt acctgttgtt
    62641 gctatcacaa atgacgtgca aagtccactt gctttagaag ctgcacaaat tattgattta
    62701 ggtgccgggg tagaatcagt aggcttcgtt acaaaaggct attctgcgac tgttttacag
    62761 ttactactct tagggatagg aattggaata agtaaaaata aaatcagcaa aaaaatagaa
    62821 caagactaca tgcaacagtt gcgcaaaata atcaaccatt taccagcgat tattcaaaaa
    62881 acggaagaat tttttgatgg atatcaaagt ttatttcgtt tagcacagcg tttcgtcacc
    62941 attggatatg gtccaaactg gggaacagct aaagaagcag agacgaaatt gactgaaaca
    63001 atccgcgttc cgtcacaagg atttgagtta gaagcttata tgcacggccc atacctggag
    63061 gcagatgctt cgcatttgct attctttatt gaaggggaaa gtgtaaataa ggaacgttcg
    63121 caaaagttgc aaagatatat gagtcggtac gttggagaga ctctcaccat taccacaaaa
    63181 aaagcaagaa acgagaaaac acttggatta gcaatagaat gtgatgaata tctgtctgta
    63241 ttagcgcttg tcgttccgtt tcaactattc gcttataaaa ccgcagtagc taaaggtatt
    63301 gacttgaaca aaaagatttt tgaagacttt gatacagtct tgaaaagtaa attataagag
    63361 gtggaaatca tgctgaaatt caatgaagaa gaacaaatca aagcaataaa aggcgcacta
    63421 gctttacgtc cgcaagtaga agaaatcatc gataaacttt atgcggaaaa atttgacgct
    63481 gtctattatc taggaatcgg tggaacgtat gcttcctcga tgcaagctgt cacttatatg
    63541 aatgggaaaa gcaacttgcc tgtctttgta cagcatgctg cagaatatta cactactgga
    63601 aacaaaagat taacaaaaga ttctatcgtt attttgtcct ctgttaccgg tacaactcaa
    63661 gaagtagtga aagcagtaga acaaatcaag aaagtaggag cgacactgat tggatttatc
    63721 gataaagcga acagtaagtt gtctcagcta tgcgattttg tggtcactta tccagctcct
    63781 ggaacagaac aaatcaaatt ttttatggca gcagatcgat tgatgtattt gaatggagaa
    63841 ttcgaagctt attcagaata ctatgaacag ctcgaacgtt atttaccaac agggttagtg
    63901 gaagcagaga aaaaagcaga tgcatttggc ttatcatttg cagaaaaaca tcgacgtgat
    63961 agtatgcatt attttattgg agcagggaat caatggggag ctgtttattc atatgccatg
    64021 tgttactggg aagaacaaag ctggcttcca tcgaaatcta tccatgctgc cgagtttctt
    64081 cacgggacgt tagagatcgt agaagaaaca accccagtca cattattttt aggcgaagat
    64141 gaacagagag tgttatctga acgtgtggct aaacttttgc caaaaatttg cagcaattat
    64201 acattgatag acacaaaaga ctatccagta gaaggtatca gtgaaaaata tcgcggacgt
    64261 gttttgtctt tcttattgat gcatgtagtg acacaacgca tcgatgctca tgtggagcag
    64321 ttgaactgcc atccattaga gatacgccgc tactatcgtc agtttgatta ttaggataga
    64381 ctggataaaa agaatataaa aaataaagag agcagcgata actgctccct ttattttttt
    64441 aaaatattgg catcaattac gttataaaac acattttttg cattatctac agttcaactt
    64501 ataatctgtt taatgggaca tgtgggtgcc aaccattttt tgttatgcaa tagtactatt
    64561 tagttgttgc atggatccgt agtaaaagtc caagtacaac gagttgcgcc agtttacata
    64621 gcgttttgta tccagaatgt tgactaaaac aaaagttcct atgtcaaccg tcaacagaga
    64681 caatctgtcc gttgcaggac ctgacagatt tagaaatgtt caatctgtct cttatgtatt
    64741 ttaaatcaat actcactagg agaagtttcc taacctccta atagtaatgc tggtagtaac
    64801 attgtcaaaa ctgcttcctc ttaggaacaa gcaaagcata acgagtgtaa aaataaaatg
    64861 tagactattg taacatatca ttgattattt caagtaaaaa cgataaaaga agtaaacaaa
    64921 cacttaaaaa caatggaaat tagtcccaac tttaagaaat gtatactata atggaaatta
    64981 tcaattattt atttggagga atatccatga aacaaaaaat cgcagttctt ggtcctggat
    65041 cttggggcac tgctttagca caaacattgg cagaaaacgg gcatgatgtc cgtatttggg
    65101 gaaatgtacc tgaacagatt gatgaaatca ataagtatca tacaaatcag cattttttac
    65161 ctgatttgac gatcccagaa tcgattatcg gatataaaga attggcagaa gcagtggata
    65221 acgcagatgc tattttattt gttgttccaa caaaagcgat tcgttcagtc gcacaggaac
    65281 ttattggtaa aatgaatact aagccaatca ttatacatgc aagtaaagga ctagaacaag
    65341 atacgcataa acgaatctca gaagttttgg ctgaagaaat tccagaagaa aaaagacagg
    65401 caattgttgt attatctggt cctagccatg ctgaggaagt ggcagtacat gatatcacaa
    65461 caattacagc tgcaagtatt gatcaaaaag cagcagccta tgtacaaaaa ctgttcatga
    65521 atgattattt caggatttat acaaataatg atgtgattgg cgtagaaact ggtgcagcac
    65581 tgaaaaatat cattgcaatt ggtgccggcg ctatccatgg attaggattc ggagatgatg
    65641 caaaagcagc aatcatgact cgaggattgg cagaaatcag ccgtcttggt gtcgcgatgg
    65701 gtgccaatcc tctgacattt atcggtttga gtggtgtggg ggacttgatc gttacttgta
    65761 caagtgttca ttctcgcaat tggcgtgccg gtaacttgtt aggacaggga cataagctgg
    65821 aagaagttct agaaaacatg ggaatggtcg ttgagggtgt ggcaacgaca aaagcagcag
    65881 tagagctagc tcaacagcta ggtgttgaga tgccaattac tcaaacaatc tataatgttt
    65941 tgtataatgg agaagatatc aaaaaagcag caaaagaaat catgttacgt gacggaaaaa
    66001 tggagaatga attttctctt cgttaaatag atgatcaata gttaatcgt
//
LOCUS       DAFCNJ010000009        62255 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000009 DAFCNJ010000000
VERSION     DAFCNJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.009"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..3044)
                     /locus_tag="LNE35_000753"
     CDS             complement(<1..3044)
                     /locus_tag="LNE35_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HBK6909933.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVS
                     AAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
     gene            complement(3661..4095)
                     /locus_tag="LNE35_000754"
     CDS             complement(3661..4095)
                     /locus_tag="LNE35_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909934.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(4112..5317)
                     /locus_tag="LNE35_000755"
     CDS             complement(4112..5317)
                     /locus_tag="LNE35_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBK6909935.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            6011..6397
                     /locus_tag="LNE35_000756"
     CDS             6011..6397
                     /locus_tag="LNE35_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6909936.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            6491..6682
                     /locus_tag="LNE35_000757"
     CDS             6491..6682
                     /locus_tag="LNE35_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909937.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            7196..7528
                     /locus_tag="LNE35_000758"
     CDS             7196..7528
                     /locus_tag="LNE35_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6909938.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7824..7979
                     /locus_tag="LNE35_000759"
     CDS             7824..7979
                     /locus_tag="LNE35_000759"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBK6909939.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            8255..9595
                     /locus_tag="LNE35_000760"
                     /pseudo
     CDS             8255..9595
                     /locus_tag="LNE35_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9836..11452)
                     /locus_tag="LNE35_000761"
     CDS             complement(9836..11452)
                     /locus_tag="LNE35_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBK6909940.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            11570..11788
                     /locus_tag="LNE35_000762"
     CDS             11570..11788
                     /locus_tag="LNE35_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909941.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11983..12444
                     /locus_tag="LNE35_000763"
     CDS             11983..12444
                     /locus_tag="LNE35_000763"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6909942.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(12637..12876)
                     /locus_tag="LNE35_000764"
     CDS             complement(12637..12876)
                     /locus_tag="LNE35_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909943.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12900..14423)
                     /locus_tag="LNE35_000765"
     CDS             complement(12900..14423)
                     /locus_tag="LNE35_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBK6909944.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(14441..14563)
                     /locus_tag="LNE35_000766"
     CDS             complement(14441..14563)
                     /locus_tag="LNE35_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBK6909945.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(14593..15576)
                     /locus_tag="LNE35_000767"
     CDS             complement(14593..15576)
                     /locus_tag="LNE35_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK6909946.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(15600..15749)
                     /gene="rpmG"
                     /locus_tag="LNE35_000768"
     CDS             complement(15600..15749)
                     /gene="rpmG"
                     /locus_tag="LNE35_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK6909947.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15770..16147)
                     /locus_tag="LNE35_000769"
     CDS             complement(15770..16147)
                     /locus_tag="LNE35_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6909948.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(16179..16358)
                     /gene="rpmF"
                     /locus_tag="LNE35_000770"
     CDS             complement(16179..16358)
                     /gene="rpmF"
                     /locus_tag="LNE35_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK6909949.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(16373..16642)
                     /gene="rpsN"
                     /locus_tag="LNE35_000771"
     CDS             complement(16373..16642)
                     /gene="rpsN"
                     /locus_tag="LNE35_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK6909950.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(17165..18931)
                     /locus_tag="LNE35_000772"
     CDS             complement(17165..18931)
                     /locus_tag="LNE35_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6909951.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18891..20644)
                     /locus_tag="LNE35_000773"
                     /pseudo
     CDS             complement(18891..20644)
                     /locus_tag="LNE35_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(20754..21323)
                     /locus_tag="LNE35_000774"
     CDS             complement(20754..21323)
                     /locus_tag="LNE35_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBK6909952.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(21341..22756)
                     /locus_tag="LNE35_000775"
     CDS             complement(21341..22756)
                     /locus_tag="LNE35_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6909953.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(22767..23435)
                     /locus_tag="LNE35_000776"
     CDS             complement(22767..23435)
                     /locus_tag="LNE35_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK6909954.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(23565..24149)
                     /locus_tag="LNE35_000777"
     CDS             complement(23565..24149)
                     /locus_tag="LNE35_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6909955.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(24480..24713)
                     /locus_tag="LNE35_000778"
     CDS             complement(24480..24713)
                     /locus_tag="LNE35_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBK6909956.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24728..25678)
                     /locus_tag="LNE35_000779"
     CDS             complement(24728..25678)
                     /locus_tag="LNE35_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBK6909957.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(25675..26517)
                     /locus_tag="LNE35_000780"
     CDS             complement(25675..26517)
                     /locus_tag="LNE35_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK6909958.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(26519..27238)
                     /locus_tag="LNE35_000781"
     CDS             complement(26519..27238)
                     /locus_tag="LNE35_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK6909959.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27930..28088
                     /locus_tag="LNE35_000782"
     CDS             27930..28088
                     /locus_tag="LNE35_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6909960.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            28160..29386
                     /locus_tag="LNE35_000783"
     CDS             28160..29386
                     /locus_tag="LNE35_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6909961.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(29474..29866)
                     /gene="rpsI"
                     /locus_tag="LNE35_000784"
     CDS             complement(29474..29866)
                     /gene="rpsI"
                     /locus_tag="LNE35_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK6909962.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29882..30325)
                     /gene="rplM"
                     /locus_tag="LNE35_000785"
     CDS             complement(29882..30325)
                     /gene="rplM"
                     /locus_tag="LNE35_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBK6909963.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30844..31311)
                     /locus_tag="LNE35_000786"
     CDS             complement(30844..31311)
                     /locus_tag="LNE35_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBK6909964.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(31431..32969)
                     /locus_tag="LNE35_000787"
     CDS             complement(31431..32969)
                     /locus_tag="LNE35_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBK6909965.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            33049..33735
                     /locus_tag="LNE35_000788"
     CDS             33049..33735
                     /locus_tag="LNE35_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBK6909966.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33936..34463)
                     /gene="msrA"
                     /locus_tag="LNE35_000789"
     CDS             complement(33936..34463)
                     /gene="msrA"
                     /locus_tag="LNE35_000789"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK6909967.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(34547..35224)
                     /gene="pgmB"
                     /locus_tag="LNE35_000790"
     CDS             complement(34547..35224)
                     /gene="pgmB"
                     /locus_tag="LNE35_000790"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK6909968.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(35221..36351)
                     /locus_tag="LNE35_000791"
     CDS             complement(35221..36351)
                     /locus_tag="LNE35_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK6909969.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(36351..38534)
                     /locus_tag="LNE35_000792"
     CDS             complement(36351..38534)
                     /locus_tag="LNE35_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK6909970.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(38531..39556)
                     /locus_tag="LNE35_000793"
     CDS             complement(38531..39556)
                     /locus_tag="LNE35_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6909971.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(39601..40428)
                     /locus_tag="LNE35_000794"
     CDS             complement(39601..40428)
                     /locus_tag="LNE35_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK6909972.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(40425..41492)
                     /locus_tag="LNE35_000795"
     CDS             complement(40425..41492)
                     /locus_tag="LNE35_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBK6909973.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(41518..42354)
                     /locus_tag="LNE35_000796"
     CDS             complement(41518..42354)
                     /locus_tag="LNE35_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6909974.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(42351..43220)
                     /locus_tag="LNE35_000797"
     CDS             complement(42351..43220)
                     /locus_tag="LNE35_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6909975.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(43290..44573)
                     /locus_tag="LNE35_000798"
     CDS             complement(43290..44573)
                     /locus_tag="LNE35_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK6909976.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(44587..46272)
                     /locus_tag="LNE35_000799"
     CDS             complement(44587..46272)
                     /locus_tag="LNE35_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBK6909977.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            46547..47572
                     /locus_tag="LNE35_000800"
     CDS             46547..47572
                     /locus_tag="LNE35_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6909978.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(48167..51841)
                     /gene="rpoC"
                     /locus_tag="LNE35_000801"
     CDS             complement(48167..51841)
                     /gene="rpoC"
                     /locus_tag="LNE35_000801"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBK6909979.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(51899..55516)
                     /gene="rpoB"
                     /locus_tag="LNE35_000802"
     CDS             complement(51899..55516)
                     /gene="rpoB"
                     /locus_tag="LNE35_000802"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK6909980.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            56244..57212
                     /locus_tag="LNE35_000803"
     CDS             56244..57212
                     /locus_tag="LNE35_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6909981.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            57230..58135
                     /locus_tag="LNE35_000804"
     CDS             57230..58135
                     /locus_tag="LNE35_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909982.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            58132..58938
                     /locus_tag="LNE35_000805"
     CDS             58132..58938
                     /locus_tag="LNE35_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6909983.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            59074..60063
                     /locus_tag="LNE35_000806"
     CDS             59074..60063
                     /locus_tag="LNE35_000806"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBK6909984.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(60380..60904)
                     /locus_tag="LNE35_000807"
     CDS             complement(60380..60904)
                     /locus_tag="LNE35_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBK6909985.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            61191..>62255
                     /gene="scm"
                     /locus_tag="LNE35_000808"
     CDS             61191..>62255
                     /gene="scm"
                     /locus_tag="LNE35_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBK6909986.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        18818 a        12513 c        10420 g        20504 t
ORIGIN      
        1 ccttctactg ttggctcata tttatcggcg tcagaacgat tgtctgtcac tgtaacatct
       61 acttcaactt catcagatga tccatctgga tatgtcacta caacagtccc ttttttattt
      121 ccagctgcgc taacatctac tttttctttc caagtatact tagtatcttt tggtaaatct
      181 tctttgttct taattccctc aaatgcgtct ggttccttat tcaattctgt agtcactttt
      241 tgaccttcag gattatattt gtcagcgtca gatttatcat ccagttttgt tgcacttgat
      301 acaaattctg tatcatctgc tgtaattggt tgagttgctt cctctggcca tttcgcatct
      361 gtataaccat cttttgcttt caacactgga agtttatctt ctggtaagct tacgccgtct
      421 ttcactgcaa agactgttgt tccactttca atacttgttc cttctcctgc ggtgaaggtt
      481 actttgtggt agcctgcagg aatgttttct cctggatttt caatgatatc atccagtttt
      541 gttgcacttg atacaaattc tgtatcatct gctgtaattg gttgagttgc ttcttctggc
      601 catttcgcat ctgtataacc atcttttgct ttcaacactg gaagtttatc ttctggcaag
      661 cttacgccgt ctttcactgc aaagactgtt gttccacttt caatacttgt tccttcccct
      721 gcggtgaagg ttactttgtg gtagcctgca ggaatgtttt ctcctggatt ttcataaata
      781 tctttactta cagttactgc taaatcggtc gtgcctacag aaccatcttg gtaaacaatt
      841 ttaataggaa ttgttccagt aatcggctcc gattctccta cacctttagc tggagtgaat
      901 gtaacaatac cagtgtttgc atcaacactt gcattaccga agtacttatt atagacatct
      961 gaaattgaaa aggcatcttt agctaaagga acatctggtt tatcaaatgt aggttttcca
     1021 ctttgaactg tcgtttctgg agttgttgtt ataggtacgt atgttgcatc attttccata
     1081 gcttgagaag cttttacagc aaactctaca tccacgtcga aagttcgatt tccgatctta
     1141 acagttacaa tacctttcgt actatcagta acgttagaag tatcaggctc tgttttccat
     1201 gaatactctg catcttcagg taagttttta ttattccgaa ttgattcttt agcatctggt
     1261 ttaaatccaa ctaccacggt ttgtttatca accgcttttg gtgattcctt aataacggtt
     1321 gaaccttctt ctggtttatc aaaacctgga gaaacgattt ggatttttaa ttcatctttc
     1381 gcgattaatt tacttgaatc tacacccgta aattcaaatt ctacagtatc tgaaattgga
     1441 gccccatctt tttcatttgg agcgatagtt ttttctgcta atacagtacc ttctttatca
     1501 acaagttggg ctttgtgacc tgttcccgct aactcgtgga tgaatacttt ccccttaact
     1561 gttgtgtcaa catcatatac ttcttctaat tcaggtttcc cgatatatgg attcgtgtct
     1621 ccgctctctt ctttatcgtc agggtttcca tcaccatcaa tatcttgcaa ataataataa
     1681 cctgttccat aagtgttctg gattaatttt ttattcgtat ccgacaacca agaatcaaag
     1741 atgaaatctt caccaaatgg tttattttct tcaacttgtg ctgttgcttt catatcttct
     1801 aagatttcat ctaatggctt agtcaattgt aaaacatatc gaataacgac tggacgtgga
     1861 cttgcattag caaaatcgat aagtttagtt aaatcattat tagttattga acctatgcca
     1921 taattttcat gaccttcccg tcgctcgatt gctaaatcag ctacttttgt tccggattca
     1981 aacgaaacat catatgttac tttgtcagcc tgaaccatgt gcatctgtat acccgctaca
     2041 tatggaacta atcgttcatc aattttgtaa ttagcatacc aaggcgtgcc ctttgcggaa
     2101 gatgaatatg agaaattata atttttcgtt aacttatgat ctaagattat agctccatta
     2161 gcacctgtgg atcctgaacc atcttttgtt tgagtattag gtttctcata ttttgcactt
     2221 actgatccat gtttaaactg gtcaggatta ttttcagata catctaagct attatagaaa
     2281 tcatctgaat ctgttaaaaa gtagcccgta ctatcaatac ccggtacaat tttatcatcg
     2341 tcttgatgac ttaccaagta gattcgatac attaaacgat cggtcttgag attgtctatg
     2401 cctatctctt taaaaatttc atctaggctc ttttcaaacg taattacccc attaggagcg
     2461 gtttgcgtat cggttgtttc tgccccagca aataatccat cacttgcacg aatataattg
     2521 accttccaaa tatttgtagg tctacccagc gaatcattaa ttcttacaaa ggtccgtcta
     2581 gaagatccaa ttcctttctt tggatcgacc tgaatttttt ctacatattt agctaatcgt
     2641 tcatctaatt gaagacggat ttcccagttt ttagttgatg ctgcaatatt atgttttccg
     2701 taaacttcaa atccaattgt attgcctttt agtgtgtctc cagttagttc ttgaggacta
     2761 aattgtaaat ccgaaaagtt ataatttttc gtataaccag gattctcctg aaaagtattc
     2821 cccgaagcta ctttgtcacc tacttgtacg gagcttatcg ctgtttcaag tgggctgctt
     2881 ggagaaattt catcttgtgc tttaactagt tcagttgttc ccaagacaag accaacacca
     2941 atcaaaacag atgcaacccc aacacttaat tttttgatac tgtacttcaa tactcgtttc
     3001 tttgtttttt ctagaaatac tctcttatta ttcttgctaa ccattattca aatcaactcc
     3061 cttaataatt actttcctag taagaataaa agataaatct gatacgtatt attcacagaa
     3121 taggaatctt actaacctta tgttaactaa tttacttaga gaatttcatg aacattacaa
     3181 caaaaaaagt acatttcttc atgttttttt cggatatttg tgctttttaa ccattcagca
     3241 gctgaaaaca tataaaaaga tatgtatgta ttctttctga aattaagttc atttctatgc
     3301 gatgaagcat gttttcaaaa tattaccctt tcttagatta aacttcagag tattcaaata
     3361 acggaataag gagccttatc ccgcattact gaatgagact gtgacataag taaaaaggtg
     3421 acttcaaatt ccttcttcgg aatttgaaat caccttcttt ataattataa agtatcggtt
     3481 tcacagcatt tctgaatgat tcctagaatc ttaaacgaat gattggagtt atcaacattt
     3541 tttcactcgt ttttagcgaa acaaaaaatg acatttgtca cagcctcatt aaattattga
     3601 ttctttttct ttataattga attgtccata tttaaagtgt ctagtactct acatgaataa
     3661 ttaattgctt tcactcaaat acttataatc tattaacttg tagttgttta acttttctga
     3721 aaaagttaga cccacgtaat aatcttccca gctttctctt tcaattaact gatccaattt
     3781 ttgataatcc ataaattcat catgtatcaa aaagaataaa taacttgata ttttagcatc
     3841 tagcttaatc agattaaata atgtttgttt aaaattgcta ccattaatat gactgataga
     3901 ttctctagct tcttcacata aactttttac tttattaatt actactttgt catattcatt
     3961 taacacacta ttattaccat tacttagacg ctcagattct acgaaacaac tcaaatcaga
     4021 aatttcccaa aaaaattttt tgttaaaatc caatttcctc aactcctcca aactacgtaa
     4081 atgatatagt ctcataataa aataaaacct ctcagctaat gttgttgaaa ttgtccacta
     4141 aacctaattg atattgctta ggagatacac ctgcgtactt tttgaatact ctcgaaaagt
     4201 gtgaagaatc tgaaaatcct aaacctaaag aaacttcact gatagtatgg tttgatttca
     4261 acattatttt agccgtagaa atttttcgct tagagatata attataaatc gtaatatgca
     4321 tttcattctt aaagctttct cttaatttag tttcacttac agaaaaataa ttagctattt
     4381 cctttacagt aattctttta tagatattta agccgatgaa ttgaacgaca gaagaaatta
     4441 agggagaaag atgctctatc ttagtttttc cgatttcctt agtataataa acaatcccag
     4501 aatccctcac ttttaagacc tcaggtaacg atacagctaa ttcgttttct tgtatcaaag
     4561 catctctact ttgatacgct tctattatat ccattcctaa tgttatagac gtttgtgcta
     4621 acttttcaaa aacaatgata gaataatttt tttcagaccg aatcgtatct ccactaacac
     4681 ttggaattat tccattactc agattaaaaa ccatgttttc taaatttcga atatctccat
     4741 ttttaacata ttccagtatt ttattttcat aaagaaacag atatatttct ggatcgtaat
     4801 tttgtgataa cagattatct attttgattt gctcgatagt tttacttaga ttttctgtat
     4861 attcaattaa cggttcatgg aatagatctt ctatttttcc agtaaaaaaa taatgaacta
     4921 aaatcagaat atctcgaatg tctcctaaag aatacaaagt caaattttgc aatgaattat
     4981 agagtcgttc tctatcagcc attgcaacat tcaaattgtt catttttagt ttaaaaaaaa
     5041 ctttatcaat attatttgct ctaaatggac caaataagta atatctggtt ttaaacatgt
     5101 atagaagaaa catctcatta aaatcaccag taaagaaatg gaaatcttta gtttttttaa
     5161 tattatcagt tagtaacttt ttataatcat aaaaaaaata agtagttcta tctgagcgat
     5221 actcttttat aatactgaat tcggaattaa aaaccacgat aggtaacttt aaacaaacat
     5281 gaagagactt taataagact tctttatctg aaaacacttt tgtttctctc tttcctttct
     5341 acatcattaa atagtatttt atacgttatc taaaatacta ttcctctgtc aaaataagct
     5401 aacttacttc attataaaat atccacttat cgctttcatg tatattccac cgtaaaaagt
     5461 acattttatc accgagaact catcattttt catcatgatg aatactattt acatatatct
     5521 agttcaaaga caataattat tatgatccgt tattatctaa gcactataca gataatagga
     5581 atttttcgtt tctaaatatt ccaatttggg aatccaataa aaattttcgt tcaaatacgt
     5641 gtctattcga actcttttct agttgttaat tatgaagggg gaagaaataa aaaatacaat
     5701 ctatacacga ataagaatat tatccaaaag gaggttttag aaaagattta ttatacgtaa
     5761 aaagctcaag acattttctt agccataagg acatgtttga tatttaagaa gatatatgaa
     5821 ttttcttttc taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa
     5881 taaacatcag cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat
     5941 aattgcatta gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga
     6001 ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga
     6061 aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca
     6121 tgaacaactt gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca
     6181 accgattaat gttttaaatg tgactctcct tgttgaagat ataaaattag caaaaatcct
     6241 tcctttgttg aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttgataaaac
     6301 tgataaagaa attggaaagg ttctggggat tacaagacag ggtgcgacaa atttaaaaca
     6361 tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg
     6421 tatgcttctt tgctcgttct cctccttccc aaagaaatag attataagtc catgggaaga
     6481 aggtagattt atgaattctt taaaagaaga tttcatttta gcaaaggctg gaaatgaaga
     6541 agctgttgag gcaattctca aaagatttag ctctttgatt cataagcaat cctggagaac
     6601 tggaaaatat gatcaagatt gttatcaaga atgtatgctt gcaatatatc tagctatctc
     6661 gaaattcgaa attaaagaat aactcaacta agggagtctg tgacataagt aaaaagatag
     6721 tttcaaattt cgaacaataa atttgaaact atcttcttta taattataaa gtatcgattt
     6781 tacagcgttc tataatgatt catagaatct aaaacgaatg attttaatta tcaacgtttt
     6841 ttaattcgtt tttagcttaa aaataacttt tgtcacagtc tcactcctaa aattacttta
     6901 tttacgtatg aagtgggcag gaggtcgaat ccagaactgg gctatggtac ttcaacgagg
     6961 acttctcctc tagaattgaa aagcatgcga tttaccttcc gtaacgacgg tagatttatt
     7021 caactatgtg tccaatcaaa atttgtctga tttcaaaaat agtcaagaga attttggttg
     7081 ttaaaaactc ttatctatca acttccacag tttagttgac aaaccctgat actaagttgg
     7141 gctatgatac acttttcttg actatcagtt acgttaccgg aaggaatgaa ctctattgag
     7201 aaaaacatat gatcgtaaat ttaaactaaa gatttcacag gatatccttg agaaaaaagt
     7261 aactaccaag aaaattgctg aagaatacaa tatctctcgt ccaacaattt cccgatgggt
     7321 ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga caaggaaaaa gattacctga
     7381 taaagcagat ttctacattc ttgaacagga aaataaacga ctaaaagagg aagacgagtg
     7441 ttctacgaac acaaagatcg atacggatct ccagggatca ctcaaaaact ttacgatgaa
     7501 ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc gtgaaatgaa tttatgcaca
     7561 agaggatttc atcgacgcaa atcttctcat ggtagagaaa aaacaatcaa agaaatggta
     7621 aaaaaatgtt gaatcgataa tttgaacaga accaaattga tgctgtttgg gtgatggata
     7681 ttacttacat tccatgtaat gacggtcgcc tctatttgtc cacctatatt gacttagcaa
     7741 ctcgtattct acgttactat atggtagatt cacacatgaa aaaagagatt gtcgtccgtc
     7801 cattacaaat ttattaagga gaattgccta cggtaattca ttccgataga ggtagccagc
     7861 atcggtcttt tatttatcaa gagctgttga acgaccacca catcacacat tcagtgagcc
     7921 aaactgggac tcctgttgac aatgcagtaa ttgaagcgtt tcatcgatca atcaaatgag
     7981 aattaatcga ctccaacaaa tataaaagta aaatagagat gaaggtgtta atccaagatt
     8041 atttagaaga ctactaccca aacaaaagaa tccatacaaa atttatgatg actccttgac
     8101 aatttgaagc gaagaaaata agggatctat aatatctagt gttggtgacg ccttctgttg
     8161 ccaatacttt ttcttttttc taataaacgc aaacagacac cttaaaatcc tttagaataa
     8221 agtcgacgaa aaccattcaa aggagtgttt caagatgtcc tattcagagt ttaccaaaga
     8281 gttactagat attctagacc taaatcttat ctttcatgaa gatgcctttc gcaaagagcg
     8341 aatcaatgac gaaacatgtt ttgtttttga tggcacattg acctatcaac cggaggaatg
     8401 ctttcatcgc cactatcaaa atgaacaaac gatcattaaa tagggctgga aaaaagtatc
     8461 catcttattg aatgatgtca gtaactacaa gaccattctg cggattaaca aacagcgctt
     8521 caaatgtaaa cactgtggaa agactttttt agcagaagat tcagttagtg atcgtcgctg
     8581 ctccattgct cgcagagtca aacaagccat tcttgagcta ttaagcgaac ctctttctat
     8641 gtctttgatc gcacgtatga agcacatttc tccgaccact gtcattcgaa tacttcgcag
     8701 ccttcggcct aaaaccattt ctttgagcca gctgttacca gaagtggtgt gtttcgatga
     8761 attcaaatca gtgaaaaatg tctcgggagc catgagcttt gtcatgatgg acggcaagac
     8821 ccatcaactg attgatattg tggaaaatcg gcaactcaat cctttacgcg actatttctt
     8881 gcgctaccct ataaaagtcc gtgaacaagt gcgtctagtg gtttcagatt tctatacacc
     8941 ctatcgcaca ttggtgaaag agtgttttcc gaatgcccga atcgtcgccg atcgctttca
     9001 tattagccaa cacattggtc gggcttttac caaccaccga attcaagtaa tgaagacctt
     9061 taaaaaaggg gatcgtcggt cgaagcactt aaagaaatac tggaaactac ttcaaaagaa
     9121 tgcgtgggaa ttgaaaggtc agcatcgcta ctggcgtccg tcatttcgag atcatctgac
     9181 agaagcagag atagtcgatc ggctattatc ttatgatgat tccttaaagc gaggctacga
     9241 agtctaccag gattttcttt ccgttatcag aagacaagat gttcctgaat tcgtcgcgct
     9301 cctaaaagaa gactacaagg agttgccaga gcactaccaa ccagtgttca cgaccttcaa
     9361 gaaataccaa acagaaatca aacgagcgtt gcgcgttccc tattccaacg gccctataga
     9421 atgtttgaat aaccatatca aagtactaaa acgaattgcc tatggctata gaaactttca
     9481 aaactaccga gaacgaatct ttttatatcg aggaaaatat tttaagaaaa ctaagaatac
     9541 tacccaactg actaaagcca gaacaacaac acggctagac aagatagctg tctagccgtg
     9601 tgagtatctc gattattcta ttgaatttct tcaccaacac tcattgacga agagccaaaa
     9661 taagtaatct aaccgactgt taactaaacc attgcaggtc ccgttaaaga tacttgtgcc
     9721 tgttgtcacc gcttcttttt cttgtccaca agcgcttaac acacttaata aagcccctgc
     9781 tattattaat aataatatct ttttcttatt aaaaacctcc tcatctattt ccagattagt
     9841 aaatgtagta atctgtgctt tcttttttct caatactaat aactaactta tggggtaaac
     9901 aaatactcgt ttgaccgggt tttgagatcc atccagttct aaccgcgatt tgatcaggac
     9961 tattatcttc tttaactcga attcttccat ctttaatctc aacaatatta tattgtccct
    10021 tcgcaggatg ataattcacg agtttatgat tgatcttatc caaatcgaag cgatccattt
    10081 ccttgccatc aattcgaacg atggcatatt tgatagcatc ttcagttgtt tcattctcct
    10141 tttctataga gaatacaaat aagggaataa aagaggtaat aatcaaaatg ccaataatta
    10201 tgacatcgcc tatcttgaca ccagcttttc ttaaaaattt cattaacgat atctggctaa
    10261 acatattaga ctttttccat ggaaatggtg aaatttttcg gaaattatct gcattaagag
    10321 aatcagttaa acaaccctca agttgttcct taatacattg ttgatccatc ttcataccaa
    10381 taaaaactag tttattaatg cgttcaccaa acaacggatg ccaggcttcc ttcaattcgg
    10441 gatttttttg tataatcatt tgacactcaa ttttaggtaa actagctacc caataattta
    10501 atgattcaat acttttatag ctacccgatg tttcaaagat cgtcgaaaaa tcactatgtt
    10561 gggcaaacca aagaaaccct tttgctcttg taatattgcc aggaaaattt ttatccgtaa
    10621 gatgggcaaa tttttgtgcg tcaaggagta accgatttcg atagataaaa cttgagatac
    10681 catattcttc tgtttcagga atatgatttt gatagccaag ctctaactct ttgaaccagc
    10741 ccactgaata ggaagtctca tctatattaa aaagatttgt atttagaatt ttcgataagt
    10801 ctacttcacc tttaattgtc ctaattattt tggcattgga ctgtaactta gcaattagac
    10861 cgttaatttt gtaaatcgtc ttggttgaca ctaagtcact attatttaaa agtaatatgt
    10921 tacaacactc gaattgatca attaacaagc cacgaatatc ctgttcttct tcaactacat
    10981 taccttcagc ttttctttcc tgaatctttt cacccgtttc gaaattatcc caaaaacgtt
    11041 ttacatccac tactataata atggcaccaa tctctaatcg ttgggtcaaa tcaatgtcta
    11101 aagggttttt agctaatata attgcttgaa taattggaat tggtttgctg attcctgaag
    11161 cttcaataac aatttgattc aagtcagaaa attgcactaa ttgatctagt tcaacaatca
    11221 aatcttcgcg taatgtacaa caaatgcatc cattcggcat cgaaacaagt ttttctccag
    11281 ttcttttaaa acctttcttg tcgactaata ccccatcaac atttacttcc cctatgtcat
    11341 ttacaatgac agcaatttcc tttccgtgat caccgtacag aatatgattt agaagtatcg
    11401 tttttcctga accaagatag ccagttaaga ttgtaatagg tatttttttc atagggaacc
    11461 tctttctata ccttgaattt acactttaag tgcaacacat aaaaatgagt cataatcgat
    11521 atccaactag tataagtttg tcgagaacac tcctagaagg aaaataatta tgagccagta
    11581 tcgtcatctt actatactcg aacgagaaag aatctttctg cttcacgaag aaggattttc
    11641 tattagtata attggcaacg acattcagca tagcccgttt actattttac atgaactgag
    11701 aagaaacaag aaaaaaacta ttcaccttcc aaagcggaaa aaaattatgc ttatcgaaga
    11761 aaaaaatgcg gacgttatca cattctaaat gatccgtgag tttggtcaat tgttcgtcat
    11821 ttatttgtcg attttcagtg gtcgccagag gaaatctcga atcgtttgaa gcatgaacat
    11881 atggactttt caattagttt tatcacgatc taacgtgcaa tttgcaatga tcttttcgat
    11941 acagaacccc tctcacacgg caataaaggc ctgatccgtt cattgcacca tcgaggaaaa
    12001 acaagacata caaaaaatca tgtcgagtct cgtggaaaga tttgccttac tcattcgatc
    12061 catggccgtc caaaatccgc agatgataga agtgattttg gtcattggaa agccgatatt
    12121 gtagctggca aaactggtgg tacttgttta gtgactttga cagacagaaa atcatgcttc
    12181 cttttagcag gaaagaaggt ccccagaaag aaagccgctt atgtgagcga gaaaatgatt
    12241 gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa
    12301 gaattctcca atcatttcgc agttacagaa gcattgaata atatgccctt ttattttcca
    12361 gatcctcatg cccttggcaa caatgaacaa attaaaatac gaatagactc attcgagaat
    12421 acttgccaaa atcgaaagag atagctacgg tagataactc aacgataaat cagatgattg
    12481 aaaaactgaa taacagacct agaaagtgtt tagattggag aacaccttat gaagtcttct
    12541 ttgataaagt gttgcgcttg atttgacaat tcaagacata ataaaaagca gatcaaatcc
    12601 aaataaggga aaaatgatct actctggtat tatcaattaa ttggccaagc tattatgccg
    12661 ttttgctaaa cgtctacttc ttccagtatt gaccttgata aaacaacaat caggatcccg
    12721 ttttaatact ctatgaactg tgtcaatact aacatgtaaa gttgcagcaa tatcagctaa
    12781 tgtactccca tttaatctca tttgctttgc aaatcgataa cgccgataag catcaatatt
    12841 cttggaatcc atagacaata atacttttct taccatgtat ttcctccaaa aattgaattt
    12901 taatgagcca tcatttcttg agcaatttca tgtttagaca atgaagctgg atagaaagta
    12961 ggccaattat gcaattcatt atagagtttt tcttgacttt ctccaccaaa gaaaatatgg
    13021 aaatgctctg ccttacttgg tgcaattcca tgatcactaa attgaatata tttaaaacga
    13081 ccagcacctg catcatcagt ttcaaaatta aaacgaacac cacggtttcc tttttcataa
    13141 ttcaaaattt tgtatccaac atatttgtat gtgtattgat gatgttcacc gttgactaca
    13201 aaatcaatgg tgttgtcttt aatgttgata gtatcaatat ctgtcttata gccagtagta
    13261 taataatctt tgtactcggc agctgtcatg tctttattga tttttgcttt gtaatcgaaa
    13321 acttcatcca aaattccatc ttttaacaac ggataaacag attgccattc gcctgtataa
    13381 tcactcaatt cgcgatcctt cacatcggca tctttaaagt acccattagc aactgtcttt
    13441 tcatccttcg cttctttttc tggaactacc tcttcaccag caaccatagt tgttttttct
    13501 aacgctttta gattgtcttc cattactgaa acataatctt caccattatc catttgttct
    13561 tttgtaagac tttcaagagg attcagtact tccaatttta tcccagcttc gttggccaaa
    13621 gtttttgcaa ttttatcatt agcattcttt tcaaagtaaa tataattgat cttatttttt
    13681 ttaacatact cttttaattc agctaatcga ccagaagatg gctcttcttc aggacttagt
    13741 ccagcaatag gaacttgttt caatccataa tccaaagcta aatatccaaa ggctgcgtgt
    13801 tgcgtcacaa aacttttttg tttcgcttct ttaagactag tggtatagtc agcatccaac
    13861 cgtttcagtt tagtcaaata cttttcagca tttgtctcaa atacttttgc ttttttcgga
    13921 tacaatttaa ctaactgatc cttgatattt gaaacttcct taattgccat tttgggtgaa
    13981 acccacgtat gaggatctag ttcatgatgg tgatcttctt ctccgtgatc atgatcttct
    14041 tcttcactac ctggcaataa aatcatgtct tttgttcctt ctactacatt aggcccagct
    14101 ttcttccaac tttctttagc ttttggtacc catgattcca tgttttcgtt atgatataca
    14161 aaaacatcag catcatgagc ttttgccata tccttagctg agggctcata gtcatgaggt
    14221 tctgatcccg ctggaaccat taaatccacg ttcgcttcat cgccgacgat gtttttggtg
    14281 aaatcataca ttggatagaa tgttgttaca atttctaatt ttccatcttt atcattcgat
    14341 acttttgcat cattagtgct ggagcaagct cccaataatc ctagagctgc tatcccaata
    14401 agtcctacac tgattttctt cattcttttt ccttccttaa ttatgctaat ttcttcattt
    14461 tatttcgttt atgttctttg ataattttta atgccctttt tctagtttta atattcgggc
    14521 ttttaagtcg tcgatatgca ctagatatat ccgttttctc cattagaaat tacccccaat
    14581 cactacttat cattatttgc tttccgccag taatttatca aaactctgtt tgatcctttc
    14641 ttcatccaac atttttccga ttagaataat agtggtttgg tctttttgtc gagtggtgta
    14701 ttgaaaatta acctgttcgt ttacctcttg catggcaact ataaaatcct gctcttgtaa
    14761 agcaatcaat cctttaaaac gatacaggct cccttggttg gttaaaatca gccagtcaat
    14821 ccattggagt aaaaaatctt tttttatgtc ttgatctgaa gttaagagaa ttgatttaaa
    14881 ctcatgtact tgatggtgtt gatgatagta tttgccatta tagtgatttt ctgatgaatc
    14941 actttcaaaa actaagggat gattaaattt tttcaaatta taaaaatcct ttttttgaat
    15001 cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg ttaatacttt tgatttctct
    15061 ctgtaatttt tttagtgcag ataaattcaa gttttgtttt ctagacacaa aaatgcgatc
    15121 tgccatgaca agttgcttaa gggcttgtgg ttctctttca ataagttcaa aactgtcact
    15181 atcaataaca gtcaataaac tatcgatata tactcttcct cttagttgag gcgctgttaa
    15241 aagtgtttga atgattggtt ggggatctgc tatacctgta gtttcaatga tgatttgtga
    15301 aattggttgt tgatgttctt ctattgtttc cagtaaagaa atcaatattg ctaataaatc
    15361 actgcgaaga ctgcaacaca tacatccatt atttagttgt aacacccgtt cctcgctatg
    15421 aactaataat ttgtgatcaa tcccaacttc accaaactca ttttcaatta taataatttc
    15481 ttccctgggg tagggtgaga cttgcaaggc ttgattgatt agcgttgttt ttccagcccc
    15541 taaaaaccca gaaacgattg taactggtat gcccattttg tcaccttttt accacttagc
    15601 tatttcactt ctttaaaaat tgcccgtctg cgcaattttg gcgaatactt ttttaattct
    15661 aaccgttcag ggttattccg tttatttttg ctggtgagat acagacgttc tccagtttcg
    15721 acacattcca aaataatatt ttgacgcata aatcttcctc cttcaatact tatcgatctt
    15781 caagatatgc ttgaatttgt tgtgcatctt caaaagttaa ctcgccagat tccaattgtg
    15841 aaactaaatt ttctttcata tcaacaggta tctcccaata accatcgata cattgaaata
    15901 actcctcgtc atactcttca aaaattggat agattcttaa aaaatcatca aatgataagc
    15961 cttggtcctc atagtaatct ttgactaatc cattttcaat tgcctgttgg gtcggtaaac
    16021 ccgtttcagt aataattcct ctctctacca tcgaacgata aaaagtgcat ttatctagcg
    16081 attgctgggt cgtttcaaga taatacttta atccttcttc aactaaaggt gggtaaataa
    16141 tttccatttt gattccctcc taaaagttat atattaaact attttgaagc tcctttaatt
    16201 actttctttc cattatagta cccttttaaa gatacatgat ggctacgtct ataatcacca
    16261 ttcgcttcgt caaatgaaat ttctggtttt actacagttt gatgagttct tcttaaccgt
    16321 ttcttcgcct tagatgtttt tcttgctggt actgccatga tatttcctcc ttttaccaac
    16381 ttgctttttt tactccagga atcaaccctt gatgggcaag ttctctaaac ttaatacggg
    16441 acataccgaa ctttcgcata taccctcttg gacgaccatc cgtctgatca cgaagcctca
    16501 gacgattcgg atttgaatct cttggcaatt tagcgagagc ggtgcgatct cctgcggctt
    16561 ttaattcctg acgtttatct gcatactttt caatcatcgc catttgtttc tttgctttag
    16621 caattttcga cttttttgcc atataccttt atactccttt tattttgatt tagacacctc
    16681 aaatcgtaat cgttacgttt tgacactgat actttaccaa caagagttgc tcttgtcaac
    16741 aataaataac atgaaataaa actcatatga atagataact atttgaaaac gaatttatcg
    16801 gtacatagag ataatcaaat catttaaaag aatatgcagt ttaggctctg aaaaataaga
    16861 attcactttt attagtccaa tcaaacacaa ataccgtttt aagtagcttt tcctctactt
    16921 aaaacggcaa tatgaaagaa actataactt tttattcaat cttttatgtt taactggttt
    16981 atcgccaatt ttttaaaact ttgattattc cttatattat aagaatagac attaagtgaa
    17041 ctaattcaat catactgatt ctttcgtgta ttcaaaatta gatgctgttt aataaaatta
    17101 ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt taaagcctat
    17161 ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct
    17221 ttatgagatt tttgtatatt tctgaccttc gatacaactc attatgagta ccttgatcaa
    17281 ctagttttcc ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat
    17341 gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt
    17401 tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt
    17461 tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt
    17521 tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag
    17581 ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat
    17641 tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa
    17701 acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata cagccttcat
    17761 cataatcata cagtctagac actaatttca agatactagt tttcccggaa ccactagcac
    17821 ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg
    17881 tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac
    17941 tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt attttaggag
    18001 ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta
    18061 ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt
    18121 gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca
    18181 tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt
    18241 tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt
    18301 ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg
    18361 ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa
    18421 taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa
    18481 acagagccat accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa
    18541 cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg
    18601 gtaaatttga caaatcgtct gcaatttgca ctcttaaatg cgcactttct ttataagtac
    18661 tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacaa
    18721 acgatacggc aaaataaagc caacggggct ttgtattttc taaaactaat tcgtctatca
    18781 gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaaa ctagaaatag
    18841 aagctttagt taaagcttct gcgccctgat cacttaaagc aaatttgttt ttataaaaac
    18901 ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac
    18961 acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac
    19021 aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat cataataact
    19081 gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca
    19141 atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca
    19201 atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta
    19261 tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca
    19321 aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga
    19381 ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa
    19441 tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct
    19501 gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt
    19561 cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt gtatgaaaag
    19621 ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc atattttagt
    19681 tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata tcttgcttca
    19741 aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat tccacttaaa
    19801 aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga agttataaaa
    19861 aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg aaaaagaaca
    19921 aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc gttaattgct
    19981 ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac ttgaattccg
    20041 cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata tttttgaata
    20101 aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa catggcgatt
    20161 agaaccaatc caattttaaa gttaatgaaa aagcctaagg caactgataa aacaggtgtt
    20221 actatggctt gagcactatc tggaatcaag tgagcaattg ccatatgtgt tttagccgcg
    20281 ttgtcatcaa ttgtctttcg aatatatccc gaagaattta aatcaaaaaa acgaaaactt
    20341 gcttctgtta aaccgctaat tccctttttt ctcaaattag tctctaagcg aaacccaagg
    20401 atatgagaaa ataagccaga aaaaatataa aataaagctc taaagttaac caagcaacag
    20461 tttgaaccgc ataactctta gctaaatatt catctcctac aataattaga gactgaagaa
    20521 atttgtaaat actgtaatat ccatagactg ttaatacgac agaaaaatca gaaaataaag
    20581 tggctaacca tccgtagtat ttcattttag ggacataact aaataatttt ttgtatactt
    20641 tcattttttc ctcccccgca ttcaatttct tctcttattc gtttacaact gttaatgctt
    20701 tccgtaatag cgtaaattag agatgtacaa acagataaaa ggtaaactac gttctatttt
    20761 ttgtattttt ttaagacttt tcttccaatc aatacaccaa tcatagataa acttgctaca
    20821 actaacactg aaatgattcc ccatatacct accgcatagt ggatcattgc atccattgtt
    20881 gcagcagatt gtcctttatc tatccaatct tgacgaaaac gatctgtaaa gaaccatata
    20941 ggaatccact gtccggcagc actgcctaat tgcattagag cccaagacgc aacttgagga
    21001 attgctcgat catatccaaa tacagataga ataatatcag ctaagatcgc catactaatc
    21061 gaactgataa accaaggtaa gtacgccccg cccattaacg taaaaaataa caataaaaca
    21121 attgtgtaaa tagtaaaaat tccttttcta gggtagttat agctaatata aacaaatatg
    21181 attccagata ataatccaaa aatcccgggg gatatgctgt gaccaaacgc cccaccaata
    21241 ctactaacca ttattgaaaa aaagtaaatt actagggaaa taactgtagc taaaccaata
    21301 aaaatataat cttttgtttt catatatatt ctcctcaatt ctatttcgtt atgtcattaa
    21361 aaatctgttc tatcaaagtt gaatcttcta ctctaagttt tatatctttt tttattccac
    21421 cattttttaa ataaataatt ctattgcatg ttttataaag aaattccgga tcatgagtag
    21481 ctataactac tgcacccttt tcttttaagt ctttaataag gtcagcaatc ctttccatat
    21541 taatataatc caacccactt gtaggttcat caaatagaaa taaattagtt tttgacaatg
    21601 aagctataga aaccaataat ctttgttttt ctccaccgga taaatcaaac ggtaattgat
    21661 ctttcatttt ataaagttcc atttttttta aaattttttc ttgctgatct ttactaacta
    21721 tttcattccc cagggctaac tcgctcctca ctgattctgt gaaaaattgg taatccattt
    21781 cttgcattac taaaaacggc gatgacaaat tttttttatt ttcgattatc tttcctttgg
    21841 tagggcgaat aatcttcatg agtattctta gtaaggttgt tttccccaca ccgtttttcc
    21901 ctaccaaacc aataacatct ccaactttga catcaaaatt tatgtctttt agaatatttt
    21961 cataagtaat ttcttcaact tttaatacat ctgcacttac cttttcgata tagggaactt
    22021 caatcgaact attaaaaatg tcaaatttcc ttaatgcgtt attgttttca gtttttaaca
    22081 actgaatagt tgtagttttt acagttctat ttttaacaaa tagaacttga tcaattaatt
    22141 ctttcaaata gtaaaaacga tgttcagcta taacaatagt aaatccttta ttttttaaat
    22201 cacaaagtaa gtcagcaagt ttcttagtcg ctttgaagtc caaatttgaa gacggttcat
    22261 ccaatataat taatttagag tttagcgata aactacatcc aatagaaatt ttctgccgtt
    22321 caccacttga tagcgaaaaa atgtttttat ctaatatatt tgaaagatct aataattcag
    22381 ctaattcatt gattctttta tccatatgct cttttgtaaa tccaaagttt tccatcgaaa
    22441 atactaactc actcgttgta tttgttgtaa agaattgtcc tctaggatct tgacttacgt
    22501 agccaatatt ttttgatata ctttcttgtg ttaaggattg tagcgagtta ttttcaaata
    22561 aaatttcccc aaccaatttt ccgtcataaa gtcttggaat aagtccattc attaacttta
    22621 atagtgtgga tttcccactc ccagaagaac cagtaatcaa tgtaagactg ccttttttta
    22681 ttgaaaaatt aaggtcaaca agcgcttgtt ccttaacatc cttatatgaa aaagaaacat
    22741 tgttaaattc aaacaattct ttcacctcaa atcaaaatac ttaagaaaaa aatagtaacg
    22801 gaaaacagga tgatatagtc taataagctc atgtttgttt tatagtaaga acttcttttc
    22861 acaggagatt ctatcccctt tgttattgca ttagcggtca tttcttcagc aatcatagtt
    22921 gcacgtataa gaacgggaac taacagaaat tccaaatatt gcagaggctg aaaaaaagac
    22981 aagcgaatgt ttctcatttt taaagattct ttaatcattc ttatttcaga acgataagtc
    23041 ggaaagaatc tgatcactac tgtaatactt aacattattt ttttcggtaa atgtattttt
    23101 tctaaagagg ataataattc attgctagta tagacactaa ccaacaacca tgagatcatt
    23161 acgaccggca acattcgaaa tacaatatat aaggatgtcg caaaaaatat agtaaaaaac
    23221 tctatcttga tagcatcaat aattttcatt actaaaaaca gaactgaata aatcaagatg
    23281 aaataaatac atgtctttat tttacgaaac atcaatacta aaactgtagc aataatgaaa
    23341 caccccactt gaaatatatt tccctttcct aaaaaaataa tgaccggaaa aaaaatattt
    23401 aaagtaatca aggttaacgg attgattttt gacactcttt cacctccaaa aaataagatc
    23461 gcactgttat cttaagctga taaaaatgca ccgttattca gataacagtg cgatcttatc
    23521 aaaaagcata actgagaaca attctcaatg caagcttttt ttaattaact cagtaattca
    23581 atccacccag gaacagaaaa ttgatacaat cttctggtta atagcatagt cttttctttt
    23641 gaatagctat gcttcaatat atcatagaat gtagcgtaga atgagtaaat gtagaagtgg
    23701 atttcttctt cattaaagac taaattttga ttgatcattc cgatagtctt taacttatct
    23761 agacaacaga ctgtcttttt cgtcacaaat gatgtaattt tttcaacaat atcttgatat
    23821 ttggttccag ctgattggaa taaaatcaaa tcgaatatac gtttattctc aaaaatataa
    23881 ttaataaatg gatctaattc attgaagttc tctctccaac ttttctctat atctatattt
    23941 tctaagttta agaggtattt ggtaaactct tcattataca tattaataag cgcattaata
    24001 tgcggctgaa taatttcttc aaataacgct tccttagaag taaaatgttt ataaaaagca
    24061 cccgtagtta cgcctgcggt caggcacagt tgccttatct tagtttgctg aaatccttct
    24121 ttttcaaaat ggcttagtcc actttctatt aacttttggt gagttataat ataatctctg
    24181 gtttctatgg gtacttcctc ctaataagat gaattaactt tattatctta cacaggactt
    24241 gaagtttcaa tcgttaacgt ttattactga actcattcct tagagctaaa ctaatgagaa
    24301 agagcagaat aactgataca aaaagcaatc ctacaagaat taatagactc atctaaatca
    24361 ttcacctttt ctaaaataat ccgagaaaga tttaaaaaaa ttatatccag ctattaggat
    24421 aagtcctatc ataatcactc caatagccat tttcttatcc tccctaaaaa taaagtcttt
    24481 tacttaaaac cttttttgta atcgtcaata aattttttcc ctttatcagt taattgaaat
    24541 cctatatcta aatagtcctc atatctaagt ttagccagca tctcacttac agatgctgga
    24601 ctaacactta gaacttttgc taaatcatta ttttttaatt tcttttttct ttttgataat
    24661 tcctgaattg ctagtaaata aattagtata gtagtcgttt taatttgtac catacctacc
    24721 ctccaaatta tttcgaaagg ccttcagcaa ttttatccag gttccatttc atcatcgcat
    24781 agtaactatc accatcttgt cctttttctg caattgaatc agtaaaaatc gttgaataga
    24841 taggaatatt ggtatctttt gatactgttt tcattggtct atcatccaca ctgctttcta
    24901 cgaataagga gggaactttt gttgtgcgta atttttcaac taagtgtttt atttgatctg
    24961 gtgttccttc ttcttcagtg ttgatttccc aaatgtaagc agaaggcaca ttatacgctt
    25021 tcgagaaata tttaaagcat ccttcacttg taacaatcat ttttttatca ttcggaattg
    25081 aagcaaattt agatttagct tctttgtcta gagaatccaa tttttcaata tacttatcta
    25141 gattttcttt atagaatttt ttattatcag gatctttttc cgctaactgt ttttcaatat
    25201 ttttagcgta aataataccg ttttctaaat ttaaccaagc atgaggatct tccttccctt
    25261 tctcactctg accctctaag taaataacat ctatgccatc acttgctgca aaatagtctt
    25321 tgttttcctc tttgttcgca tttttaacta attttgtaaa ccaagcattt cctccagttt
    25381 ccaagttaac accgttataa aaaatcaaat ctgcttttga agttttttga acatcttcag
    25441 gcaaaggttc atattcgtgg ggatctttcc caataggtac gatactgtgt aaatcaattt
    25501 tatcttttgc tatattttcg gtaatatctg ctaaaatcga attagtaact actactttta
    25561 atttttcgtt cgaagccact ttatctttgt ctttactatt tattgaacat gccccgaata
    25621 taaaaagagc aatcaaaatc cccagacttc ccagtactgc tttccatttt ctcatcataa
    25681 cgatctctcc ttctttctta aaaatttttg ttttggtgag attagaaaac taattacaaa
    25741 taagaatgcc gctgttaaaa caatacttga tcctgctgct atgttaaaac tataaccaat
    25801 aaacaatcca agcaatgatg aacttgctcc gactgttgca gaaataaata tcatagtttt
    25861 caagctattt gcatataaat atgctgtagc tgctggtgta attaacattg caacaattaa
    25921 tatagttccc acactttgca tagctgttac tgctactaaa gttaatagaa ccatcaatag
    25981 ataatgataa aaactgacat tcatccctat tgcttttgcc attaatggat caaaagaagt
    26041 gattaataat tctttaaaaa atagaactac tataaccaac actaaaaccg aaacgccaat
    26101 tgtaatccac ttatcaatat cttgtacagc taaaatattt ccaaataaaa tatgaaataa
    26161 atccgttgag ctatttgcaa ctcctataag gataactcct agtgctaaaa aagaactgaa
    26221 agtaatacca attgctgtat ctccttttat tacactattg ctttttataa aagtaataat
    26281 gatagaagct aatagaccaa atacaatcgc accaataaaa aaattaatac ctaaaatata
    26341 agaaattgct actcctggca aaacagcatg agaaatagca tcacccataa gagacattcc
    26401 tcttaaaata ataaaacaac ctactgttcc agaaacaatc cctataacta cagatgtaat
    26461 tagagcattc tgtaaaaagt gaaagtcata cagaccatta ataaaatttt gaagcatatt
    26521 attccccctc tccaataaat atagtatctc cataggcttt ttttaaattt tcttcattaa
    26581 atacagattc tacacttccg tgagcaatta gatttcgatt caaaagaata atattatcaa
    26641 aatacttctt taccttgctt aaatcatgat gcacaattag cactgttttt ccctgatgtt
    26701 tcaatttttt taacgtatcc ataattattc gttcactaac taagtctatc cctacaaatg
    26761 gttcatctaa aaatatgaaa tccgcatttt gagcaagaca tcttgctaaa agtacacgtt
    26821 gaaattgtcc tccagataat tctcctattt gatgattaga atattttccc atatcgacct
    26881 tttccaaagc ctttgaaact ctttgccatt ctgcattttt aattctttga aaaactttca
    26941 ttccggcata tgttcccata gatacacatt cttttacttt tattggaaaa gtaaaatcaa
    27001 tatcagcttt ttgctctaca tatgcaatct tcaccagttt tttattgata tcttcattat
    27061 taatggagac ctctccctga tgagggataa tatttaacat tgctttaagc aatgtggatt
    27121 ttccagcacc atttggacca ataatacctg ttaaagcacc ttcttgtatt tttaaagaaa
    27181 tctcgttcaa tgctaaaaaa tcgttataac aaacattaag ctttttcact tctaacataa
    27241 ctttctcctt ctaaatgata atgattatca cgatcatatg gtatacaaaa attatatttt
    27301 agtcaaccct aaattttata gtttagattt ttttctgttg ctgaagatta gaatgacaaa
    27361 actatttgaa actacgcgta tagtaagtag ctttcaaaat atataaaaaa ggagcgttct
    27421 caaatgagaa catgctccta aaactacttt attggcgtat ggaaaaatta tacttgaagt
    27481 ggatagcggg ttgaataagt tgataagcat caaaacattt ataaaaatta ttcaagacta
    27541 tgtctgttcc atatacaaat atgaaaatca gtaaactcca tttaactttt ttcgtagtgt
    27601 gtctttttat acatgtttat cgttattaat agtgaatagg aaaaaaacta tttttgatca
    27661 ttgacaatta aatacaattt ttgaataacg atccctgcta ttcgagctag aaactcaagt
    27721 acgccatgac ctgattagcg acaaatactt agagatttct ttttgttctg ggaaaacaaa
    27781 atgccatgac aatagcagca aataatgata cttccgtaag gctcgccgta aactggggag
    27841 aggtgaaatt cctatgtgct tacccaaagc aattcaaaaa tttcataact actattttag
    27901 gagatgattt tatagaatcc agagtctacc tgaataaaaa acaaacctgt gcatttctag
    27961 gaatcgataa taatactcta gataagtgga ttcgtatata taatttccca gtaataagag
    28021 ttggtggtgt agttcgcttt gacgtagaag atattaaaga ctttatgaat acatttaaaa
    28081 taacttgaaa ttttcttctc gccgcatata ctaaaattag tgaaatatac tgccttactt
    28141 tttttaagga gtgaggattt tgcctacaaa gctttcaaat gggaaatata aaactaatct
    28201 gagatatcca aaaaggttta aagaaataac aggaattgca tcagaaaagt atcaaaaaac
    28261 atttccaaat aggcaattgg cgattaaagc cgaaaatgat atgaagaaga aaatagaaaa
    28321 agttttgaga gaggagaatg caaactcatt agaattaaaa ggaaaaataa cgtttaaaaa
    28381 attttacgaa tctaaatggc ttcctagata tgaattaggt caaacaatta gatcaaacag
    28441 gcctcctagt gatataacca ttagtaatac aaaagatata tttcggttgc atattttacc
    28501 tatgtttggt gaatacgcaa tgaattatct aaatatcaat actgagataa tatcagatga
    28561 attaaccaaa aaatcgaaag aatatgcaaa tataaaaatc ataaaaggat atgttcgttc
    28621 catgttcgat attgcagaaa tactaaatta tatcgaattt aatcgaacta ctaaaattat
    28681 tcaaagtatc actgcaccta aaaaaaatgc tttagaagaa aaaagaatac aagaaggtaa
    28741 gcaagcacta tcatcaaaag aacttactaa ttggatagaa gcagtaaatg atgattttaa
    28801 taatcattta cttacttttc atgattacac actttttatg attaccctgt atcttggtga
    28861 tagaaaaagt gagacttacg ctttgcaatg gaaatatatt gattttgaaa aacaaacggt
    28921 ccgtcttaaa catactttag ataaatatca aagaaaaaag tttacaaaag gtcgaaaaga
    28981 taccgtcatt caagtaccag aagttgtaat gacattgcta agtgaatgga aatcggtaca
    29041 agctgatcaa ttgttgaaat taaaaattaa gcaaacgtta gatcagtatc tctttactta
    29101 tactaaacca tctggagaag taaattgtcc tgttcatgcc gattacctaa actatcgaat
    29161 caattcaatt aaaagaaggc atcctgactt agttcattta tctccacaca aactccgtca
    29221 tacttatgca actatagcta gacaaggtgg agcagatatg aatcaaattt caaatgcttt
    29281 aacacactca gatatttcta caacaaagat atatgtgaac acaccagata tagttgataa
    29341 ggctgttttt gaagcttttc agagaggatt gaataagtgt gattgatatg tgttcttaaa
    29401 tgtgttctta aaaacaaaag aagcactttc caaataattg aaaagtgcca gaaacgttga
    29461 tatataaagg atattaacgt tttgagaatt gtgaagcttt acgtgctttc ttaagacctg
    29521 gttttttacg ttcgaccata cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta
    29581 aacggaagtc aggatcaact tctagcaatg cacgagcgat accatgacgg atagctcctg
    29641 attgtccagc gtatcctccg ccgtttacgt taacaaatac gtcatatgcg ccttttgttt
    29701 cagtaacagc gaaaggttga ttgattactt cacgcaagtc agcatgtgga atgtattctt
    29761 caacatcttt tttatttaca gtaattttgc cagttcctgg tactagacgt acgcgagcaa
    29821 ctgcgttttt acgacggcct gtgccgatat attgaacttg tgccaatgaa attccctcct
    29881 attagattaa gtttgtgatg tctaaaactt ctggttgttg tgctgcatgt ggatgttctg
    29941 ctccaccgta tacgttcaat tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca
    30001 taccttttac agaagtttcg atcaaacgac gagaattttt agcacgcaat tcgccagcag
    30061 tgattgattt caatcctcct gggtagttgc tgtgacggta ataaattttg tcagtcgctt
    30121 ttttacctgt taatttcact ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca
    30181 aatgaggtgt gaaagttggt ttatttttac cacgtagtac agatgcaaca actgttgaaa
    30241 gacgtcccaa tggaacatca gttgcgtcta ctacatacca tttacgttct acttcgcctg
    30301 gtttggccat atatgttgta cgcacgttat tttcctccaa tttaagttct gatgtttgac
    30361 atacacttcg agtttccggg gctctttgtg gggcaaacaa taccatttaa catattaccc
    30421 gctaatctag tcaatgtcaa cgtttttctg cttgattgaa aaatttatat gaaactttac
    30481 ttcttttctt tcttttatat aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg
    30541 ctgtgacaaa agtcttgtca caacccctta ttttgaataa acggtgttct cctgccaact
    30601 cctcggtcac aagtcacaat atccataaaa cataagaagc tgttttttaa aattgtttct
    30661 tgtgactcgg agctgaacgg caggcttaca acctcttggt aaacggtgtt caatcagctg
    30721 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgac gtttgtttct
    30781 ctaatactca ggtcaaaacg ctgatttcac aacctctttt tcggtcaagt tcaacaacag
    30841 tttttacgcg tcgatctctg gcgctttgcc caaatgagct tggatcatcc agatacgttt
    30901 ttctgtggct gttttgaatt cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc
    30961 agaaacttcg attccttttt ggtaaagatc tgctagataa cgatagcctg caacaagtgt
    31021 ttccaaacgt tctggaatag tacgatccca gcggccaatt tcatcaggga tttttgtgtt
    31081 atctgcaaat tcacgcaatg tagaataagg tgaaccgtct aatgtgatca agcgttcgga
    31141 tacttcatct aactgagcgt tcaaatcatc catgaactca tccatcattg ggtgcaatgt
    31201 aaggaaacct ggtcctctca tgtaccaatg tgtttggtga acaacaacag acatttggct
    31261 tagatctgca actaattgat ttaatacttc tttggtttgt tggaatttca tctttcttgc
    31321 ctcctaacat tttcttacac ttaaaataat agtagacttt caatgaaaag tacaacaaaa
    31381 tcatttacct gagaacgatt ttcagttcgc ttttttatcc gtaaagccaa tcatttttca
    31441 gaatcagcta attcttctcg cagttgagca aattgctgat aagtctgtgc taaatgcttt
    31501 tgtgtcgaaa caaataatgg atagattttc tgatattttt tatgatcagt ttcttgtact
    31561 ccaacttgct ctttcaattc tatcaattcc tgtgcttcac tgatactttg gatctttccg
    31621 atgcttctcc agcctaaaat cacagctcct aatgcggatg cttctgtgac agtagggaaa
    31681 acaagcgggt aacccagtac atcagccatc atctgacgaa aaatcggaga ttgagcaaac
    31741 ccgcctgtag cacggatctc tgtgagctga ttttcctggc aaactccttt taaaatccga
    31801 taaaggttga gacagatccc ttctagaatc gccttcatca tttctttttc tgtatgttgc
    31861 cgctgtaaac cgataaaact gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca
    31921 agtaaatagg gatgaaaaat cagaccattg gcacccgctg gcacaaaagc gatagccgaa
    31981 agcagtgcat catatggatt ttctcctttt tctttagcag attgaacgac ttcctgtaaa
    32041 aatgtatgcc ctgcccaatc aaagatgcca gcaccattgc tagtcgctcc tcccactacc
    32101 caatgtttct gatccagaac ataacaaaac gtctccattt ctggatggaa ctgaggctga
    32161 gtagtaataa aacgaagtgc tccgctcgtt cctacggtca aagcggcgat cgtatcacca
    32221 aaagcaccta accctagatt agaaagcggc ccgtctgccc ctcccaaaac aaatgatata
    32281 tcatttggca aacctagttc tgctgctgtt tttgatggta gacctgttac ttgataggtg
    32341 gaaggaaaga cttgcggcaa ttgctctatt cgaatgttca agtagtcaag cacttcttct
    32401 gcccattgga attcatggat atcaaaatat ccagttcctg aagctgttcc gtagtcgctg
    32461 atcagttcgc caaacaatct ataaaagata tagtctttga taccgacaaa ataagcagtt
    32521 tttttgaata attcaggttg gctgttttgc agccacttga ttttgttcaa tggtgtcatt
    32581 ggatgaatcg gcgtcccagt ctgccgataa aattctttgc caagcacggt atatttcagt
    32641 ttttctgctt cgtccgctgc tcggttatct gcccaagtga tcattcgaga taacggtgtt
    32701 ttctcttcat ccaacaatat caggccatgc atcgcagagg agaaggagac aaattgaatg
    32761 attgtgtctg aaggcaactg tttaattact tcttttataa cggtgcagac accttgaaag
    32821 atcaattcct gatcttgttc tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa
    32881 taagcggatg ctgcttcttg ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt
    32941 ccgatatcta atccgattga aacgttcatt ttttcacctc aaattttatg tatgtacaaa
    33001 aatttagttt aagcttgcgt atcttggttt tgaaaagggg gagatggcgt gttactttat
    33061 tttatcattg gttgctgtat cggatcattt ttatgtttaa ccgcccagcg gattccgtta
    33121 ggtcactcta ttatttatcc tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg
    33181 tatgaattga ttccgattct ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg
    33241 tgtcggttgc ccttctacta tttgttggca gaagccttat gtggtggtct ttttgcctgg
    33301 ttctttactt tttcttccgc aagaaatttt tcgacgttca tatggatgct ttcggctttg
    33361 ctattttcgt tgatggattt attttacttc atggtccatt ctcacacgct attctgttct
    33421 tgggcaattt tatggatttt ttggttgcag gccggcgtct tccaatggca aagtgtgctg
    33481 ttaacgttta tcgtaggcgg tgtgtgcctt cgctatggtc aatcctatct cggagctggc
    33541 gatactctgt tgatgctttc ttggagtggt ggcttgtctc tagaagagtt gcttcagatc
    33601 ttgtttcttg ctagtctgtt tggaatatcc gttttttctg tttactttat ctgtcataaa
    33661 aaaaagttgg aaaagctgcc tttcattcct tttttgagca gtgcgttgct gattgtcctt
    33721 catcttaggt attgaaaaaa ggttgtgaca aacgacttgt cacaacctct gatatatagt
    33781 gttctcttac caaatccttg tgacttggag ctaggcgaca ggttcacaac ctctccacta
    33841 aatggtgttc aatcagctga ccctcagtat tagctcaaaa tgctggtttt acaacctctg
    33901 atatacggtg ttccataagt agctttttcg gcttcttatt tcttgacgcc ccaatgtcga
    33961 cgaaggaaac ctgcacgtcc agaaccttct cgataaagat tgtaatggag cttgtttttc
    34021 ttgtaataat cctgatggta atcttctgca ggataaaatt cgcttcttgg ttcgattttt
    34081 gtcacgattg gcttatcgaa tcgcccgctg tcttgcaatg cttgttttga acgctctgca
    34141 atatttcgtt cttcttcatc attatagtag atcactggac gataagaatc accgcgatca
    34201 gcgaactgtc ctaatgcgtc agtcggatcg gtttgctgcc aatagatttc caccaactga
    34261 tcataagaaa taacttctgg atcatattcg atctctactg cttctgtatg ccctgttgtg
    34321 cctgttgtta cttgttcata agttggattt ggtacatgcc cgccagtata accggatgtc
    34381 acagaaatga tccctggctg ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct
    34441 gcgaaaactg ctttttttgt catataaaat cccccttttt tctcatcata ccttgatttc
    34501 caaaaggagg ataacgtttt gcttgtgaca gaaaaaacgg aaactattat atacgtgata
    34561 cggcttctga taaatgccaa aacgaacgta cttgatcaaa aacggttaaa tcaggaatca
    34621 catattcagc agaaggaaga tttatgtttt cacctattcc caatacatgc atgttaccag
    34681 ccaaagctgc ttgacaacca gcttctgaat cttcaatgac caagcatgca tttggcggta
    34741 cattcaattg ctgtgctcct ttgagaaaaa cttctggatc aggttttgct tttgatactt
    34801 gcgtgccgtc aataaggaca tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc
    34861 gtgcattttt gcttgctgat cccaatccga tcttgatgtt tgtttttttc aagtactcaa
    34921 gaacagctat tgcaccaggc aaaacggatg tttcatccat tttttgaatc gcttgaagat
    34981 atagattgtt tttttgctca gcaaaggctg atttctcttt ttctgagaat ttctgttcca
    35041 tttttccaaa tgacagcaat cgctctagtg actcggttct ggatattcct ttcagttgct
    35101 cgttttcttt ctcagtgaat ggaatggaca gctggttagc taaaaccagc cacgcttcgt
    35161 aatgataacg agctgtgtct acaagaacgc catctaaatc gaatagtgct gctttgattt
    35221 tcatcgatta cctccttgaa aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt
    35281 gcgatgaaga ttatcagtgg cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag
    35341 gatcggaaaa acggccgaaa tgccaacatc tgctgctttt tcgcttcctt taccaacact
    35401 tccgcaaata gctatcacag gaacgccctc tggtactctt ctagcaatcc caactggcgc
    35461 ttttcctttt agactttgtc catctagacg tccttcgcct acgattacga ggtctgcttg
    35521 ctgtacccgt ttatcgaact ccagtaaatc caatacgtag tcgatgccag agactatttt
    35581 ccctgaagca aatgctaaca gtcctgcacc cattccacca ccagcactac tgcccgatgc
    35641 ctcgattact tgaggggcaa atctcatata aaaatcatgc atggcttggt cagccactgc
    35701 taattcattt tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc
    35761 gcataaaaga ttagtaacat cagtaatgac agtgattttg gctttaatca gctcaatagg
    35821 cacgttcgtt ttatcaactg tagcaatttt acctagattt tctcccagtg cttctacttt
    35881 ttgattatct tgatcgtaaa aagcataacc tagaccttct gccatgccga tgccgccatc
    35941 gttggtacta cttccgccaa cgccgatgat gatgtgtcga acaccttgtt gcaacaattg
    36001 tcgtatcagt tctcctactc ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg
    36061 gacatctttc aatccgacga tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc
    36121 gtactggatt tctttgtttt tgccaaatgc attagtaacg actgctgttt gccgcttcaa
    36181 ccttaaactg tctgctaatg atgaaaccgt tccttctcct ccatcgccta taggtagcag
    36241 atcatattca gcctgaacaa aaacttcttg aaaacctgat tgaatggctt gtgctgcctc
    36301 taaggctgtc agactttctt tgaaagaatc tggcgcaatc agaatacgca ttattttccc
    36361 ctccgttcca agatcagttc tttttccagc tggtattggt ctccattgac aaaaataggt
    36421 aatgtttctt tcgtctcttt ttttattact atttgttttt ttgtcaaaac aaatagcaac
    36481 ctgtgatttt tccaaataaa aggaaaagaa attttttccc atgctgaagg aagtcttgga
    36541 gaaatagaaa gagttccatt cgttactgta atacctgaaa aaccaaatat aactgctagc
    36601 catagtgctc ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg atctggtcct
    36661 aaatcaatca ggcacgcttc ttggaataaa tgatacgccc attccatttc ttggatacgg
    36721 caagcctcta tggcgtgaat cgctttactc aacgatgagt cgtggattgt tttgttttca
    36781 tagtaatgca aattcttaga aacgattggt tgagaaaaat actcaggtaa taagtataac
    36841 agcatgacaa gatctgcttg cttgctgact tggagttcat ttacttcgct acgggcataa
    36901 tcaagtaaaa tagactggct gccaggacgt tctttgtaag tggtcaaatc aataactggc
    36961 ttactgagat acgtgtcatc ttgcggaatc agctgttctt tgttaggcac tggcaaatat
    37021 aacttatcta aaaaattttg acattctttc aaaaaaagcg gatcgccagc ttgatattta
    37081 acagctaagg ccacattata atgcgccaga tagttcgtat atgcattatt gtcgatatat
    37141 tctgtgtatt catctggacc aatgactctg tgtatctcta aatgatcatc gacttttaca
    37201 gctctgctca gccaaaaagc tgctgtttca tgaatgagcg tttttccctt tgtttctaaa
    37261 aacgattgat cctctgttgc ttggacatac tcttgaacgg cataagcaag gtctgcactg
    37321 atatgatgtt ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt gatattcaat
    37381 gcagcaaatt tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca
    37441 ccttgatagt gataatctct tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac
    37501 agcagcaaat tctttgctac ttcaggaaac tgatataaaa agaaaggcag gataaaaatt
    37561 tccgtatccc agaaaacatg ccctttataa ccttcgccag ttagtccctt cgcccctaca
    37621 ctaacacttg gatcattgcg gggagtcata atctgtaaat ggtaagcagc gaagtccatc
    37681 gctaactgat cgaaagaaga atcgccttgg atcgttatcc gatgctgatg ccaaaaatct
    37741 gtccaataag acatcgactg ctctaaaatt tctgtataac taacgctttt ctgggcttgg
    37801 gcaatacttt tcttaaacgg gtctgtttgc tgaagagagg tagagagcac gatgatcttc
    37861 tccagcgtaa aggacgtatt ttttctgatc ttttctgaaa aggaagcggt gatccggcga
    37921 ttttttgctt ggaatacacc tgctttattg aatttagctg caatcccaat atcaatttta
    37981 ctttctgttg tctggtagga tgcactgatc aaatcgtcac caaatactcg cagttctttt
    38041 tcaaccaact gctgtgtacc aaaattggtt tgctgtccat cgatacccgt tttcacatct
    38101 acttggcagt cttcggtcaa cggcttgatg accactttca gggctgcctg atggagatta
    38161 ttttgatcag caacacgaca agtaatgact tccacttttt ttccttgagc agtttcaatg
    38221 agcgcatagc gtttaagctc tcccatttct aaatctagtg cccgctcgta tgcaagaact
    38281 ttcatcgtct caagagaaaa atgttcaccg tcgattttta tttcccatct gatgacatct
    38341 gggagattca ctaactcggc tgattctgct cctaaaggtc ggttatatac accagctgca
    38401 aacattcccc ttacttgatt ccgatagtct tcctcctggc ttcctctgat accaaaaaat
    38461 ccatttccaa tggtcatctg actcgcatac ttattgatat actctaaatc aaactgtttt
    38521 tcttctactt tcataaggta ataacttctc ctgtttctgc cgaacgatat aaattttcca
    38581 ccagattctg gatgacatat ccttgccagc catctgcaat catctgagtc tggtcaccga
    38641 gcgcccgatt cacaaaatga gccatactta gttcatgtgg gtcattaact ggcgtggtct
    38701 tttgtaagga aaccagcttt ccatctacat cttcgaatag ttcgcctgga tacagattag
    38761 cacctgcctg atcgccacaa aaagccacat tcagtttgtc ttctctgata ttcaaagcaa
    38821 acgaagtatt cagttggatg atcttgccgt tttctaattc cagtgtgcca aaaattgcat
    38881 cttctactgt gaatttgctt ggatcccatt tcccgaattt ccctgaattt tttttcgtac
    38941 caattttttg gaagctgtac gcccgaacgc tttttacttt aggaaatcca gtaatataca
    39001 tcgctgtatc aagcatgtga atgccataat cgatcaaagg cccgcctcct tgaagttctt
    39061 tgtcgataaa cgttccccag cctggaacac cagaccgacg aagcgcattc gcttctacgt
    39121 aataaatatc cccgagaaca tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac
    39181 gatgctggaa tccataagcc aaaatctgcc cgtttttatc ggctgtctct gccatctttc
    39241 ttgcttgatc agctgtcata gctggtggtt tctcgcacat cactgaagca ttagcttcga
    39301 gggcagccaa tacatgttcg aagtgaaagc gattgggtgt acagacactc acgatatcgg
    39361 gagaaatctc attcagcatt tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc
    39421 ggcaaaagtc ttctgctcgc tggctgactg gatcaaccac agcggccaat tctaagacat
    39481 ctgatctttt ttgataataa gcagcatgta ctttttctgc tacttgtcca gcgcctatga
    39541 ttgctacttt cttcatttat ttctctctcc tttgcttatt tacttattgt tattctttct
    39601 ctattctttc tctaaatcat ttaaatgacg cgctgtttcc agacagtttt tcaagtattg
    39661 caatgattcc tgataaattt tcacttcatt ttctcctcgc aaacgacatt catataccat
    39721 ataaccttca tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc
    39781 agatcctggt tggtagcgat gattatctgc taaatggata tgacccacat actctttttg
    39841 cgtagtcaat gcttttgaaa tatcatcttc ttcaatattc atatgataaa aatcagcgat
    39901 gatctgcaca tgtttcaatt gattagagtc aatgtaatga cgcgcatccg ctaacaaatt
    39961 gatcatatgg tcttggtatc ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc
    40021 ggctacttta tctaaataac gaagtgattc gctgactgct ttatagtctc cttctttgct
    40081 tcggggtgaa accatcggcg gtaatcgata agtgaacatt ccccaagctg ctgggataac
    40141 gattcctttg cctccaacct cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc
    40201 attaagacgc cgttcttcga taaagtcacc gatccaccca tcgtagccgt tacaagctgt
    40261 cactactggg atccctgttt ctttgattgc ctgtttgact gtgtcgatat tttctattaa
    40321 caacttaccg tcgatttcgt attcttcaaa tccacagtct ttgataaatc taaatttttc
    40381 taaaatgtct cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg ttctcctcct
    40441 cggacttgaa cgcccatttt gatactttct tctgggtgct ggtctacata acgcatgtaa
    40501 gcttctgcgc tttctgcaaa tggcacaact ggatcaatga tttcttcaca atccaagtaa
    40561 ccgttcatca ataattccca acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa
    40621 tctgggtttg gttcactaga tgctcgagca aagacgattt ttgcattatt gaaatgtgct
    40681 tcgcgaccaa gattaaaccc agctggaaaa ggtttagcaa aggcaacata agaaatgatc
    40741 ccgccatagc cgatccctat taaagaagct tgtaatgcgg cagcactgcc actcgtttca
    40801 ataactgaat ctactccacg tttatctgtc aattccttta gacgaaaacc gacatctgct
    40861 ttcataggat ctagcgtgta atcggcaccg tgtttttctg ctaattctct acgctgcgct
    40921 atgggatcga tcccaacgat ggtagtagca cctgcttttt tagctaattg aatcgcaatt
    40981 tgcccgatag cgcctaaacc gaagattgct acagcatcac ctgctcgaac atgtgcttcc
    41041 cggataccac ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt ccattgatcc
    41101 aaccgatcaa gtttacgtag tttataatta tcaacaccgt ttgtaatcag cgtttcacga
    41161 atgggtccat aagtgcagac taactctcct ttttgatact ctgtcaccaa actgcccgtt
    41221 tcttcgatcg taccgacaat catatttcct aattgaaatt cgccaaatac cacaccgcgt
    41281 tggcttcctt cttctcttgg caagaatagg cgccattctt ctgaaaattc ttcatcgata
    41341 aatggactta atcctctaaa gtcgacaatt tctgttcctg gttttggagt agcgaaaatt
    41401 actcggatac gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt gaatccggct
    41461 attcttggtt ctaatgcaac taattttttc atcttttctt tcctctttct tttcttttta
    41521 tccttttacg ccgccttcga ctaaatcacc tttaataaat cgttcagata acgcatacat
    41581 aatgatgact ggtaaagcgg tcaccaatga agcggccatc attcgtcccc aaatgtagtc
    41641 tggtgtactg aataatgtgt tcaaaccgat tggtaaagta aatttttcgg aggtcgacag
    41701 gaaaatcgaa gcaaataaat aatcattcca cgaaaccata aagcaataaa cgaagacaga
    41761 aatgattcca gaaatagcca atggtataat gatccgaata atgatctgga tccggctaag
    41821 accatcgatc attgctgctt cttcaatatc agtcggaatc gtgtcaaaat aactttttag
    41881 catgaatatc gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt
    41941 atcatacaaa cctagaccgg aaatgatttt aaataatgga acaacgatca aaataccgga
    42001 aaacatataa acggtgtaaa agctagcatt gattttcgag cgagctttga agcgtagttt
    42061 tgacaaagcg tatgccccgc tgatcccaat cactactgca gttacggagg ctatcaagga
    42121 gatcactaaa ctatttttga aatacatgat gaatggaaaa atctcagcat tgaaaatatc
    42181 ctgataatgt tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg caattgcttc
    42241 ttgtgaatcc ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa
    42301 tagcaacaaa atcgtcgcgt aaaataagat tttcctgctc tttttatttt tcatttcaag
    42361 tccaccttct ttcgtgagag caagacgacc gctgcaataa tgatgaacaa tagaatcgat
    42421 atagctgctg cttttccaag atcattgaac gcaaaggctg tctgatacaa ataaacgcct
    42481 aagatattga ctttattcgt cagtagatag acatctgtaa acatatagaa catccagata
    42541 gatcgtaaaa caatcacagt tgctagtact ggtttgattg ccggcagtgt cacgatcttg
    42601 aacttctgcc aagcatttgc accgtccatt tcagctgctt cataaagcgt tttgtcgact
    42661 gtttgcaaaa tagccaaaaa tgaaataaac gcatacggat agtatctcca gatagcaaac
    42721 aaaacgacca taaagaagct agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa
    42781 agctgcaacc tatctactaa cagatcattg acgattccat aactattatt gaacatatat
    42841 ttccaagcaa aaatcaaaga aatagatggc gtaacataac ttaataaaat cagtgaacgc
    42901 accgttttcc taaatctgaa cttgcggtta agcaaaatag ctacggctaa tcctaaagct
    42961 gtactgcctg ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga acgataaaat
    43021 tcactatcac ttaaaatttc aagataattg cttaatccga taaaaacggc gtctgagtta
    43081 ggattcagcg gcatatcgaa aaaactgatc tgaatattgg aaagcattgg aatcagcact
    43141 aaaacagcta ataaaagcat gcttggtgct aaaagtccca tggctaattt taagtcagaa
    43201 tgtttccttg gacgattcat tgaaacctcc ccctgataat ctgccaatag ctatctactt
    43261 ttttcgaaca ccattggcag aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa
    43321 gcttgatcga catcttcgtt tccgacagtt acttggttca ctgcttttcc gatcacacct
    43381 gagcttgtga tatctcccat tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt
    43441 acttcatcaa aagcactagc aatctcagat ggcagctcgc caaaagactt gatgacttca
    43501 ttttctttgt aaccatccag ttcgactact tgtttattca ctggttgagc accaccagga
    43561 gacatcaaga cccacttttc catgtttttc ggttgagata agaaagctgt gaatgcttca
    43621 gcagcctctt tttgtttctc gtctagacca gaagaaatcg tcaatccaga tactgtgcca
    43681 tagacagctt gatctttttg tgttgggata gcaaaaccga tattttctga gttccccgct
    43741 tcataaacag aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt tccattcatg
    43801 aaagcatctt tgatttctgt cacatcatta gagcctggca tcgtgtattg ggataagttt
    43861 tgataaaacg acaatgcttc tctcatcttt tccgtatcga tcgttacctc gccttcatca
    43921 tccaaaacat ttgctccatt tgacaaagca aattgagaga acgcttgctc actcattgtt
    43981 ccttcagcag taggcatagc aattccatat tgtttattct ctttatctgt gaaatgttga
    44041 gcaattttta aaatatcatc ccaattttct ggttctgaaa aaccagcttc tgataatttt
    44101 tctttgtcat accaaatacc ttgaacccag ccgctaattg gtgcagcaat atagcttttt
    44161 ccatcttctg aacgaacaag gtttttggct ccgtcataat aattatcttc tccagcttcc
    44221 tcgataacgt tttggactgc cgtctgatca atcagttcat ctttatccat cactttagca
    44281 aaatcctgac tgacttccat tacttctggt aatttaccgg aacgagctaa cgtaacgatt
    44341 tttgtattga acgctgattc ttctaccggc acttgcttga ttttgatgtc tggattttct
    44401 ttttcaaaat cagcaaccag ttcattgatc acacttaatc gttcctgttc gacagaagag
    44461 tgcatgaatt gaatcgttgt ctgttgtgat tcgttagagc tttgactttc accacaagca
    44521 gtaagaagaa atccagtagc tagcagacag gtagtaacaa taattttttt catttttttc
    44581 cacctcttat tttattattt tcagccacat atattcatac ggctggaata tcaactttcg
    44641 ttcttgactg caatggataa tttgtccaga aaacaggtct aggtaatctc cttttggtaa
    44701 aagataacct gctgattgat tggaaaaatt atggataact aagactttct cttccttcgc
    44761 ttcgcgaata aaactgataa aagaatctgc tgagtccagt aagtaaatca aactattcgg
    44821 atggaaggct ttttcttttt ttcgaataga tagcatctgg gtcaattcac catatactgc
    44881 tgttcttaat ggatctgtct gcaactcttt ctcaatatct gctaacaaaa atttttgccg
    44941 attgattttt cgattctctc ctgttttttc tacgcctttg taatcatttc gacttcccag
    45001 cacactttga atataaattg ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc
    45061 taaaaagcgt ttgcttctcc attccgtcgt atctgttgga gaacttaacg catctagata
    45121 cgtagcgttt atctcatatg gactggtcgt tccatctggg tttttcttgt agtttactcg
    45181 ggcaccctct ttcttcagct gttccactaa ttcaaggatc gttttttccg gcagaatacc
    45241 tcgtaccgga ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt
    45301 tttcgatgcg aaatcgatgg tttttaacca ttctttcaac ttacttgtat tacccgaacg
    45361 aatcgcttga agggtcaaag gtggtagcga aaactgatag accatctgtg cttcattctc
    45421 gccattccca aaataactga tattatcttt gtgtggcaca tttgtctcag tgatcaatac
    45481 cgttcctgga gctgcttcat cagcgattgc acggaataat tggatgattt tatgcgtttc
    45541 tggcaggtgt atacaagaag ttcctagttc tttccataaa aatcccacag cgtccaagcg
    45601 aataaatgaa gcgccttttt ctaagtaaaa caaaagaata tcgatcattt tcaataaaac
    45661 ttctggattt ttaaaattca agtctacttg atcttcgcta aaagtcgtcc atacgtgttt
    45721 ttctttcccg tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg gtctagtcac
    45781 gctgcttgta tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa
    45841 gtttaaatat tcctgaaacc aagtactttc ggcagacata tgattgcaga caaagtcgaa
    45901 cattaaccgt gtttcgcttc tcatttcttc tatatcttcc catgtgccca aacggtcgtt
    45961 gattgcctga taatttacaa cggagaaacc atcatcagat gtatatggga aaaatggcaa
    46021 gaaatgtaca actctaaatg tatctgccaa atacttttga taaaatttct taaatgtacg
    46081 caaagttggt tgctgctctt caaaaaattg atccccataa gtaatcaaga aaatatcttc
    46141 ttcgtcccaa tgcagtttgc ctgcctggcc tttcttctgc tgataaacac tgactttttg
    46201 ttcaatcata gaggcggttt tctcagacaa atctgagtcg cgataaatcg cttgtaaaag
    46261 ttttgtatac atcggattcc cctttctttt ttgggagcgt tcccaacgat ttgatcatat
    46321 tatggaaaac gcttactgtc aatagcattt ttttaaataa ttttgatata cacaaaaata
    46381 caaataagaa agcttatgta tcaataatta atagcttctt aaaaaaatat ggtctctcat
    46441 gatcatgcaa gctattccta aattttcaaa atcatagaca aaaaagaatg tctattgtac
    46501 tatggaatta ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga aacctactat
    46561 ttatgacatt gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag tattaaataa
    46621 ccagccaaat atctctgata aagcaaaagc aagtgtactt caagcaatca aagaattgaa
    46681 ttatgaaccg agcaaaattg caagaggact gtcaggaaat ggttttgacg cgattctagc
    46741 attatctcat cgtacttctc atacgaccta tggcaatcct tttttttcag atgttattca
    46801 atccatttct gccgtttctg aacaaaataa cttcgacttg attctacaaa cggcaaaaac
    46861 accagaagaa gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat
    46921 tattatttta agctctacga tggatgaaac acttcttttt gaattggatc aatataatat
    46981 tcctattgtg gtaatcggaa aagtcggaaa cgactacacg aacatctact cagtggacac
    47041 ggataatttc caggatagtt acgatttggt ggaaacactg atcaagaatg gacaccgtca
    47101 tatcggctgt ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta
    47161 ccgtcaatgc ttatttgatc accacctaga tatccgtaat gaatggatta tagacggcgg
    47221 ctttcgctta gaagatagca tggcagcaat gaatgattta atgaagctac gagaaagacc
    47281 aactgcgatt ttttctaccg atgcattaaa atcattgagt atttacaaag tggcagaaca
    47341 atctggaaag cagatacctg atgatctttc gatcgcagga ttcagtgatc ccttattgtc
    47401 ctccctattt tcgccacagt tgactcggat agacattccg acaaatcagt taggaatcaa
    47461 agccacagaa cttttattcc aactaatcca ccaccaatac cctgaagaaa aaagaatcat
    47521 tctttcaacc gagattactc tcacagattc gatccaacaa caagaaatgt agaacagaat
    47581 tataaaagaa cagaaacact gaggataagt cagttactct gttctttttt caatcactca
    47641 tccctcgtta ccccctcccg gtattttagc gaatttttct tattttccat ctgttttaga
    47701 tagctgtgga taactataat gtttgtgtta aactattttt tccacaggga agaaaaaact
    47761 atttttaacc acgttgtgta taagttttta tagttcgttt ttttaaacac atttaccctt
    47821 atttcaatcc atgttatttg gggatactta tctctaaaac ataaatagaa taacagacat
    47881 gtctactggt tttgttttat tcgaggaatt attcaataat agctagcaaa atattaaaaa
    47941 taaccgctct atcttcccat caatttattt tgcattcaaa aaatgatcta tctttacttt
    48001 tgttgattga cttctcttgg aagatgatcg ttccgatacc ttaattggat acgaaccgat
    48061 gttgttaaga tcctttccca cttttcttct agttagacaa attattttag acaaaccaaa
    48121 aaagaggttg tgacaataat ttgtcacaac ctctttttta aaatatctat tttgtttgat
    48181 tgttcattgc gtcttctgca gccatttgcg cttcaatatc tgaaatactg tagacatttt
    48241 cgcttgctac accgacttct tttggttcca tgtcacgata acgttgcata ccagtaccag
    48301 ctgggatgat cttacctata ataacatttt ctttcaaacc aagaagagga tctttcttac
    48361 cgcggatcgc agcatccgtc aagacacgag ttgtttcttg gaatgatgca gcagataaga
    48421 aactatttgt ttctaatgat gcttttgtga tacctagtaa gacaggtcga gaagttgcag
    48481 gaacgcctcc tgagactagc gtattgtagt tttgatcttt aaagtctgcg atgtctaata
    48541 atgtacctgg taagatatct gtatcacctg gatccattac acgaacttta cgcagcattt
    48601 gacgaaccat tacttcgata tgcttgtcgc cgatttctac cccttgcata cggtaaacac
    48661 gttgtacttc acgaagcaag tagttttcaa cagataatac atcgcgaact tgcaacaatt
    48721 gttttggatc gattgatcct tctgtcaatg gtgcaccacg gtggatcata tcgccttctg
    48781 ctactttcat acgtgctgta taaggaacag tatacgtacg tgtatctgtt tttcctttga
    48841 ttgttacttc tttggtacgt gttgcaggat cttctgagat atcgatgaca tcccctgtta
    48901 cttctgtgat cactgcttgc cctttcggat tacgtgcttc aaagatttct tggacacgag
    48961 gaagaccttg agtgatatcg tctccggcaa ccccacctgt atggaatgta cgcatcgtca
    49021 actgagtacc aggttcaccg attgattgag cggcgatcgt accaactgct tctccgactt
    49081 caacgtcaga gccagttgca aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg
    49141 tattacatgt gaagacagaa cggattgtca ctgtttcgac acctgcatca acgatttgtt
    49201 ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat caattcattt gtttcaggat
    49261 gacgtacttc tttacgggtg taacgtccca ctaaacgatc ttctaatgat tcgatgattt
    49321 catttccctc gcggatggct tggatttcaa gacctcggtc agtaccgcaa tcttcttcac
    49381 ggatgattac atcttgggcc acgtcaacca gacgacgagt caagtaacct gaatcggcag
    49441 tcttcaaggc tgtatcggtc atccctttac gagccccgtg agttgagata aacatttcca
    49501 agacggatag tccttcacgg aagttcgaaa tgattggcaa ttccatgatt cgaccattag
    49561 gagcggccat caaaccacgc attccggcaa gctgtgtaaa gttggaaatg ttaccacgag
    49621 ctccagaatc agacatcatg aagattggat ttttcgcttc caaaccttcc atcagttttt
    49681 gttggatgct gtcttttgca gcattccaaa cagcaatgac acgttcataa cgttcgtcat
    49741 ctgtgatcag accacgacgg aattgttttg taatagtttc aacttgttta tgggcatttt
    49801 caatgatttc ttgtttttca ttcagtacac tgatgtcagc gatccctact gtgataccgg
    49861 catgtgtcga atgtttgtac cctaagtctt tcatacggtc aagcatctta gaagtttctg
    49921 ttactttgaa tcgtttgaag acttctgcga tgatgtttcc taagtttttc ttcttgaacg
    49981 gtaatactaa ttcttgctct ttgatatgtg caggaatatc agtaccagct tcaacaaagt
    50041 atttgtccgg tgtttggacg gtcaagttga agtctgttgg ttcattcaga taagggaatt
    50101 caggaggcat gatctcgttg aagatgatct taccaactgt tgtgatcatc gtacgttctt
    50161 tttgccattc agtaaatggt ttttcaccta atgagttagg gttcacaccg atacgtgaat
    50221 gtaaatgaac atatccgttg cgccatgcga taacggcttc gttttgatct ctgaagacca
    50281 ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct agaaccatat
    50341 cttgagaagg agtaacaact ggtttaccat ctttagggtt caagatgttt tgagcagcta
    50401 acatcaacat acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg acagccattt
    50461 ggtctccatc gaaatcggca ttataagctt cacatacaag tggatgcaaa cggattgcgc
    50521 gtccttgtac taaaactggt tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc
    50581 ggttcagcaa tactgggtgt tccttgatta cgtcttctaa aacatcccat acttcgtctt
    50641 cttgacgttc gattttacgt ttcgcatttt tgatgtttga agctagttcg cgttgaacaa
    50701 gctcacgcat cacgaatggt ttgaataatt caatggccat ttcttttgga agtccacatt
    50761 ggtacatctt caagaaaggt ccaacaacga taacggaacg acctgagtag tcgacacgtt
    50821 tacctagcaa gttttgacgg aaacgacctt gtttcccttt caacatatga gaaagagatt
    50881 tcaatgggcg gttacctggt ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg
    50941 catctactgc ttcttgcagc atacgttttt cattttgaac gatgattcca ggtgcattca
    51001 agtctaacaa acgtttcaaa cggttattac ggttgattac acgacggtaa agatcattca
    51061 agtcacttgt tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa tctggtggaa
    51121 taacagggat aacgtccata accatccagc ttggttgatt tcctgaagcg cggaatgctt
    51181 ctaaaatatc caaacggcgg attgcacgtg tacgtttttg tccttgtgca gatttcaatt
    51241 cttctttcaa ttcggctgct tctgcatcta gatcaacatt atcaagtaat tgtttaatcg
    51301 cttctgcacc catagcagct tggaattctt gtccatattg ttcacgtttt tcgcgatatt
    51361 cgcgttctgt caacaattgt tttttctcta aattcgtatt tcctggttcg atgactacat
    51421 atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct catgtctaat acaagaccca
    51481 tacgtgaagg aatcccttta aagtaccaga tatgtgaaac aggagctgct aattcaatat
    51541 gtcccatgcg ttcacggcga actttcgaac gagtaacttc cactccgcat cgatcacaaa
    51601 cgattccttt ataacggata cgtttgtatt ttccgcaggc acattcccag tcttttgtag
    51661 gaccaaaaat ccgctcacag aacaaccctt cacgttcagg ttttaaggta cgatagttaa
    51721 tcgtctcagg tttttttact tcaccgtaag accagcttct gattttttcg ggagaagcca
    51781 aacctatttg catactttcg aatttattta catcgatcaa aaggggttcc ctccatttca
    51841 tttaatggca aaccgcaaga cggctgatct tcgaccgcct tgcagctcat agaccggatt
    51901 agtctaaatt atcttctgcg gtttcaaagt tttggatgac atcgtctttt tcatcttcca
    51961 cttgttcagc agctttcttt tctaattctt tggcagtttg ctgttcggca aattttgtca
    52021 aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg
    52081 tttcttcgat atccaagaca cgcatgtcca gacctagtga ttgcaattct ttcaccaata
    52141 cgcggaatga ttccggtaca cctggttttg gaattggttc acccttgacg attgcttcgt
    52201 atgttttcac acgtccgact acgtcatctg atttgtatgt caagatttct tgtaaagtat
    52261 aagccgcacc gtaagcttcc agtgcccata cttccatttc accaaaacgt tgtccaccaa
    52321 attgagcttt acctcccaat ggttgttgtg taaccaatga gtaaggtccg attgaacgtg
    52381 catgcaattt gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca ccgacagaca
    52441 cacgtccatc aaatggttca cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg
    52501 ccataccggc ttcacgaact gtttcccata cgtcttcatc acttgcacca tcaaatactg
    52561 gcgttgctac gtggatacct aactgacgtg cagccatacc caagtgtaat tccaatactt
    52621 gcccgatgtt catacgtgaa ggcaccccta aaggattcag catgatatcg attggtgttc
    52681 catcaggtag gaatggcata tcttcttccg gcataatacg ggatacaacc cctttatttc
    52741 cgtgacgacc ggccatttta tccccttcat ggattttacg tttttgaacg atataaacac
    52801 gaactaacat gtttacgcct ggtgacaatt catctccagc ttcacgagta aagattttca
    52861 catcgtgaac gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac
    52921 gagctttttc gccaaagatc gcatgcaata gacgttcttc tgcagataat tctgttaccc
    52981 cttttggcgt gactttccct acaagcaaat cgccatcttg gacttctgca ccaatacgga
    53041 tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg atttcgcgtg
    53101 tgatttcttc aggtcctaat tttgtatcgc gggcttctga ttcatattct tcaatatgga
    53161 tagaagtgta tacgtcatct tttaccaaac gacggctcat gatgatcgca tcttcatagt
    53221 tgtacccttc ccaagtcatg aaaccaacta aaacgttttg gcctagagcc atttcgcctt
    53281 gttccataga tggtccgtct gccagagtat cccctttttc gactttctca cccaaatgaa
    53341 cgattggacg ttggttgtag cttgttccgg agtttgaacg acggaatttt gttacatcgt
    53401 atttatctaa tgcaccgttg tcgcggcgta cgcggatttc agaagcatca acgtattcaa
    53461 cgacaccgtc atgtttacat aacaaagcag ctcctgagtc atgcgctgat ttgtattcca
    53521 taccagtacc aacccatggt gcttgaggat tgatcaacgg cactgcttga cgctgcatgt
    53581 tggcacccat caaggcacgg ttggagtcat cgttttccaa gaaaggaata catgctgtcg
    53641 caacggcaac tacttgttta ggtgatacgt ccatgtagtc tactttatca atagatactt
    53701 ctaagttttc actttgagca cgagccataa caacgtcttg agcaaatgtt ccatcttcat
    53761 tcaatgggct gtttgcttgg gctacgatgt aatggtcttc gatatcagct gtcaagtagt
    53821 cgatttggtc agtaacacga cctgtttcac ggtctacacg acgataaggt gtttcgataa
    53881 atccgaattt atttactttc gcataactgg acaaactatt gatcaacccg atgtttgggc
    53941 cttcaggagt ttcgatcgga cacatacggc catagtggga gtagtgaacg tcacgaactt
    54001 cataaccggc gcggtcacga gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt
    54061 gtgtcaattc gccaagaggg tttgtttgat ccatgaactg tgacaactga gaagaaccaa
    54121 agaattcttt gatacttgcc acaactggac ggatattgat caattgttga ggtgtcaatg
    54181 tttcagtatc ttggatcgac attctttcac gaaccacacg ttccatacgt gctagcccga
    54241 tacggaattg gttttgtaaa agttcaccga ctgaacggat acgacgattt cctaagtggt
    54301 cgatatcatc gacattgccg ataccttcca tcaagttcaa gaaatagctc attgaagcaa
    54361 tgatgtcggc tggacgaacc gtttttacag ctgcttctgg atagccgttt ccgatcacgt
    54421 ttacttcacg ttcagggtct cttggtgaaa atactttgat gacttggaca gtcattggat
    54481 ctgtgaccac accgtcttct gatggatagt aagtcactga atttaggcca ttatcaatga
    54541 aaggagctag tgtttccatg acttggtgag tcagaactgt tcctttttca acgatgatct
    54601 cgcctgtttc tggatcaact aatgtttcag ctaatgtcag attcaataga cgtgttttca
    54661 aatctaattt tttgtttact ttgtaacgac caacgtttgc taagtcgtaa cgttttggat
    54721 cgaagaaacg agcattcagc aagttacgtg agctgtctgc tgtttttggt tcgcctggac
    54781 gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt gcgtttttat
    54841 gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg
    54901 tatcatcgga accaaatcct aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac
    54961 ggtcgatacg tacataagag atgtcttttg catcagtttc catttctagc caagctccac
    55021 ggttagggat aacagtagaa ccgaatcctt cttttccgtt tttgtcgact tttccatgga
    55081 aatagacacc tggtgaacgg actaactgag aaacgattac tcgctctgcc ccgttgatga
    55141 tgaatgttcc ttgttctgtc attaatggga aatcaccgaa gaatacttct tgtgctttga
    55201 tttcgccagt ctcgcggttt gtcaaacgca atgttacatg taatggtgca gagtagtttg
    55261 catcatgcgc acgagcttct gctactgtat actttggttc tttcaattca taatctacaa
    55321 attccaaaga tagattgcca ttgaagtcat cgattggcaa aatgtcttca aacatttctc
    55381 taagtccctc atctaagaac cattggtaag agtctgtttg aatttcgatc aaatttggta
    55441 attccaacac ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta
    55501 ctacgtgtcc agccaagctc ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc
    55561 aattgttaca ataatatttc aaatattatt tttaggtaac aacagacatt ttttgcggct
    55621 tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct
    55681 acttttgttt ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca
    55741 tcagatagtt tttgcaactt catatactac acttattttc tataaaagtc aacacttcct
    55801 ggcgtttctt ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt
    55861 atctgacaat tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt
    55921 tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac
    55981 ttttacttta gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg
    56041 acagtttgta tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa
    56101 aatgctaagt ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc
    56161 aatttatttt ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc
    56221 ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc
    56281 tgctactagc tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac
    56341 aaaaaacaat tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag
    56401 gattcaaaga aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt
    56461 atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag
    56521 aagatcctga tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa
    56581 caaccactat cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca
    56641 catccaatga aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa
    56701 aacaaatcgg cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata
    56761 acaacggtga agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag
    56821 cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga
    56881 aaagtctagc taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg
    56941 cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag
    57001 ttgatcaagt caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggca
    57061 aacaaacagg gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg
    57121 tcgaaacagc gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca
    57181 tcaatccaga cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt
    57241 gctctctctt ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg
    57301 tgtatacgtc actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc
    57361 tttaggtgca gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg
    57421 tcttctaggc tttttagggg gtgcggcagc agggcttgtt tccggactgc ttcacacaaa
    57481 attgaaaatt cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac
    57541 atcccgtgtg atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg
    57601 ggtcgagtca ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc
    57661 tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg
    57721 tgcaactggc gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa
    57781 gatcatcggt tatatgattt ctaatggatg cattgctctt tctggctcct tacttgcgca
    57841 aaacaacggc tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc
    57901 tattatcatc gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt
    57961 cgttttaggt gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga
    58021 accaagtgat tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca
    58081 gatccagcaa aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac
    58141 gtcttaacat tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat
    58201 gtcctgaaag gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga
    58261 aatggggctg gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag
    58321 ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa
    58381 tccatcagtc gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa
    58441 gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa
    58501 ccacaagacc gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat
    58561 cgtttatcct cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg
    58621 atggccacat tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac
    58681 cctgctactt ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc
    58741 actgctttta tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc
    58801 atgctgcatc aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca
    58861 gtgccccaat tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa
    58921 gtacttttat cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc
    58981 ttttttctct ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac
    59041 tattttgttc tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca
    59101 acgaaagaca aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag
    59161 ttggcacagc aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa
    59221 aaggcaggct atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta
    59281 ctagatgcaa aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa
    59341 acgatgttga cttctgaatc tacgatgaaa gatgccaaac tggcagcagc tcaaggagat
    59401 tctgtttcga agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg
    59461 ccttttttcg ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa
    59521 cggtttgatg aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt
    59581 gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga
    59641 aaaaaagtct gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt
    59701 catatcatca ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat
    59761 ctccaagaaa aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag
    59821 ctaatcagca aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg
    59881 gacttctata aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga
    59941 acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca
    60001 aatgaacaga agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa
    60061 taattatcag atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc
    60121 tcttcaaaag actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt
    60181 attctaaaaa tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca
    60241 agagagtcat cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta
    60301 tagatagacc atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag
    60361 ttctttttta gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct
    60421 gaccagagtg catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg
    60481 gtggtgtcca gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt
    60541 aagaaatagc aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag
    60601 cttggatctg ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg
    60661 tcatcggaaa ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat
    60721 cagcaatctg gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga
    60781 tctgcggggc tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa
    60841 acaaacgttc aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat
    60901 ccatttattt tcctcctttt tatttatcct acttactttt tcctaattct aggataccta
    60961 cttatatgtt actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt
    61021 tggagattga aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga
    61081 acgtttcatt aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag
    61141 gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa
    61201 gggcaagttt actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg
    61261 tttcagcgga aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta
    61321 actatggctt gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg
    61381 tgaatgcaca tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg
    61441 gtacagagct tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt
    61501 ctttcgctaa ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg
    61561 gcagtaacgt ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg
    61621 atcacttaac tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg
    61681 agcaggaatt tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta
    61741 aaacatatac tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt
    61801 tatcagatac cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc
    61861 cacttcaaat cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt
    61921 ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag
    61981 atttttcgga tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca
    62041 gtgctccttt tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc
    62101 aagacggaac gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc
    62161 ctaacgaaat atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag
    62221 atacatctac cacaagtgat agctcagatt catca
//
LOCUS       DAFCNJ010000010        60873 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000010 DAFCNJ010000000
VERSION     DAFCNJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.010"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            197..2731
                     /gene="secA"
                     /locus_tag="LNE35_000809"
     CDS             197..2731
                     /gene="secA"
                     /locus_tag="LNE35_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBK6909987.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2857..3958
                     /gene="prfB"
                     /locus_tag="LNE35_000810"
     CDS             join(2857..2928,2930..3958)
                     /gene="prfB"
                     /locus_tag="LNE35_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBK6909988.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4063..4749
                     /gene="ftsE"
                     /locus_tag="LNE35_000811"
     CDS             4063..4749
                     /gene="ftsE"
                     /locus_tag="LNE35_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBK6909989.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4733..5626
                     /locus_tag="LNE35_000812"
     CDS             4733..5626
                     /locus_tag="LNE35_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6909990.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5778..7226
                     /gene="cls"
                     /locus_tag="LNE35_000813"
     CDS             5778..7226
                     /gene="cls"
                     /locus_tag="LNE35_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK6909991.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7563..8420
                     /locus_tag="LNE35_000814"
     CDS             7563..8420
                     /locus_tag="LNE35_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBK6909992.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8535..9455
                     /gene="pstC"
                     /locus_tag="LNE35_000815"
     CDS             8535..9455
                     /gene="pstC"
                     /locus_tag="LNE35_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBK6909993.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9455..10339
                     /gene="pstA"
                     /locus_tag="LNE35_000816"
     CDS             9455..10339
                     /gene="pstA"
                     /locus_tag="LNE35_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBK6909994.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10354..11160
                     /gene="pstB"
                     /locus_tag="LNE35_000817"
     CDS             10354..11160
                     /gene="pstB"
                     /locus_tag="LNE35_000817"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK6909995.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11176..11934
                     /locus_tag="LNE35_000818"
     CDS             11176..11934
                     /locus_tag="LNE35_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK6909996.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11947..12624
                     /gene="phoU"
                     /locus_tag="LNE35_000819"
     CDS             11947..12624
                     /gene="phoU"
                     /locus_tag="LNE35_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBK6909997.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12862..13893
                     /locus_tag="LNE35_000820"
     CDS             12862..13893
                     /locus_tag="LNE35_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK6909998.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13890..14657
                     /locus_tag="LNE35_000821"
     CDS             13890..14657
                     /locus_tag="LNE35_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK6909999.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14757..16325
                     /gene="liaX"
                     /locus_tag="LNE35_000822"
     CDS             14757..16325
                     /gene="liaX"
                     /locus_tag="LNE35_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBK6910000.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16622..16942
                     /locus_tag="LNE35_000823"
     CDS             16622..16942
                     /locus_tag="LNE35_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBK6910001.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16942..17292
                     /locus_tag="LNE35_000824"
     CDS             16942..17292
                     /locus_tag="LNE35_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK6910002.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17459..18397
                     /locus_tag="LNE35_000825"
     CDS             17459..18397
                     /locus_tag="LNE35_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBK6910003.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18412..19242
                     /locus_tag="LNE35_000826"
     CDS             18412..19242
                     /locus_tag="LNE35_000826"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBK6910004.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19286..20311
                     /locus_tag="LNE35_000827"
     CDS             19286..20311
                     /locus_tag="LNE35_000827"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK6910005.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20326..21264
                     /gene="galU"
                     /locus_tag="LNE35_000828"
     CDS             20326..21264
                     /gene="galU"
                     /locus_tag="LNE35_000828"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK6910006.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21389..21829
                     /locus_tag="LNE35_000829"
     CDS             21389..21829
                     /locus_tag="LNE35_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBK6910007.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21832..22434
                     /locus_tag="LNE35_000830"
     CDS             21832..22434
                     /locus_tag="LNE35_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK6910008.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22493..23599)
                     /locus_tag="LNE35_000831"
     CDS             complement(22493..23599)
                     /locus_tag="LNE35_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK6910009.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23809..24810
                     /gene="ccpA"
                     /locus_tag="LNE35_000832"
     CDS             23809..24810
                     /gene="ccpA"
                     /locus_tag="LNE35_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBK6910010.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25147..27492)
                     /locus_tag="LNE35_000833"
     CDS             complement(25147..27492)
                     /locus_tag="LNE35_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK6910011.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27771..28673
                     /locus_tag="LNE35_000834"
     CDS             27771..28673
                     /locus_tag="LNE35_000834"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBK6910012.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28886..30661
                     /locus_tag="LNE35_000835"
     CDS             28886..30661
                     /locus_tag="LNE35_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBK6910013.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30727..32313)
                     /locus_tag="LNE35_000836"
     CDS             complement(30727..32313)
                     /locus_tag="LNE35_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBK6910014.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32564..32788
                     /locus_tag="LNE35_000837"
     CDS             32564..32788
                     /locus_tag="LNE35_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBK6910015.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32930..34681
                     /locus_tag="LNE35_000838"
     CDS             32930..34681
                     /locus_tag="LNE35_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6910016.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34681..36465
                     /locus_tag="LNE35_000839"
     CDS             34681..36465
                     /locus_tag="LNE35_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910017.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36791..37642
                     /locus_tag="LNE35_000840"
     CDS             36791..37642
                     /locus_tag="LNE35_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6910018.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37696..39447
                     /locus_tag="LNE35_000841"
     CDS             37696..39447
                     /locus_tag="LNE35_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6910019.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39451..39621
                     /locus_tag="LNE35_000842"
     CDS             39451..39621
                     /locus_tag="LNE35_000842"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6910020.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39661..40563)
                     /locus_tag="LNE35_000843"
     CDS             complement(39661..40563)
                     /locus_tag="LNE35_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6910021.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40794..42197
                     /locus_tag="LNE35_000844"
     CDS             40794..42197
                     /locus_tag="LNE35_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK6910022.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42197..43630
                     /locus_tag="LNE35_000845"
     CDS             42197..43630
                     /locus_tag="LNE35_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK6910023.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43775..44701
                     /gene="trxB"
                     /locus_tag="LNE35_000846"
     CDS             43775..44701
                     /gene="trxB"
                     /locus_tag="LNE35_000846"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBK6910024.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44915..46642
                     /locus_tag="LNE35_000847"
     CDS             44915..46642
                     /locus_tag="LNE35_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBK6910025.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46657..47007
                     /locus_tag="LNE35_000848"
     CDS             46657..47007
                     /locus_tag="LNE35_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK6910026.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47099..47959)
                     /locus_tag="LNE35_000849"
     CDS             complement(47099..47959)
                     /locus_tag="LNE35_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBK6910027.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48051..48479
                     /locus_tag="LNE35_000850"
     CDS             48051..48479
                     /locus_tag="LNE35_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6910028.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48641..48817
                     /locus_tag="LNE35_000851"
     CDS             48641..48817
                     /locus_tag="LNE35_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBK6910029.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48844..49290
                     /locus_tag="LNE35_000852"
     CDS             48844..49290
                     /locus_tag="LNE35_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBK6910030.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49444..50823)
                     /gene="brnQ"
                     /locus_tag="LNE35_000853"
     CDS             complement(49444..50823)
                     /gene="brnQ"
                     /locus_tag="LNE35_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK6910031.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51529..52500
                     /locus_tag="LNE35_000854"
     CDS             51529..52500
                     /locus_tag="LNE35_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBK6910032.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52524..54716
                     /locus_tag="LNE35_000855"
     CDS             52524..54716
                     /locus_tag="LNE35_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBK6910033.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54733..55209
                     /gene="ybeY"
                     /locus_tag="LNE35_000856"
     CDS             54733..55209
                     /gene="ybeY"
                     /locus_tag="LNE35_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBK6910034.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55193..55588
                     /locus_tag="LNE35_000857"
     CDS             55193..55588
                     /locus_tag="LNE35_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBK6910035.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55603..56502
                     /gene="era"
                     /locus_tag="LNE35_000858"
     CDS             55603..56502
                     /gene="era"
                     /locus_tag="LNE35_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBK6910036.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56645..57457
                     /gene="recO"
                     /locus_tag="LNE35_000859"
     CDS             56645..57457
                     /gene="recO"
                     /locus_tag="LNE35_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBK6910037.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57841..58758
                     /gene="glyQ"
                     /locus_tag="LNE35_000860"
     CDS             57841..58758
                     /gene="glyQ"
                     /locus_tag="LNE35_000860"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBK6910038.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58760..60835
                     /locus_tag="LNE35_000861"
     CDS             58760..60835
                     /locus_tag="LNE35_000861"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBK6910039.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19861 a        10760 c        13027 g        17225 t
ORIGIN      
        1 aacttgcagc agctactgac agctgccgca agtttttttg agtttcctcc tcttttagat
       61 gaaatatgaa taaattatga agttcttact ttaatacgct taagatattt caattatcta
      121 taactaatga taaaatgaaa agtaaagtct acatagtctg gataatacac agaaggaaaa
      181 ggagattaca caacgcatgg ccaatttgat tcgttcactt atcgaaaacg ataagaaaga
      241 actaaaacgt ttagatagca ttgcaaaaaa agttgaagca tacgctgacc aaatggcagc
      301 attaactgat gaacaattac aggcaaaaac tgctgaattc aagggcagat accaaaaagg
      361 agaaacatta gatcaattac ttccggaagc ttttgcagtc gtccgagaag cagcaaaacg
      421 tgtattgggg ttatatccac accacgtaca attgatgggt ggaatcgtgc ttcacgatgg
      481 aaatatccct gaaatgcgta ctggtgaagg taaaaccttg accgcaacga tgccagtata
      541 tctaaatgct ctgactggcg aaggggtaca cgtagttacc gtcaatgaat atttagcaac
      601 ccgtgactcg acggaaatgg gcgaacttta taatttctta ggattaacag tggggttgaa
      661 tatcaactca aaatcttcgg atgaaaaacg cgaggcttat aattgtgata tcacttatag
      721 tacgaataat gaattaggat ttgactactt gagagacaat atggtcgttt accgccatca
      781 aatggtacaa cgtccgctga attatgccat tgtcgatgaa gttgactcaa tcttgattga
      841 tgaagcgaga acgccgctga tcatttcagg gcaagcagaa aaatccacag ctttatatac
      901 aagagcagac aacttcgtga aacgtttgaa agaagaagaa gattataaaa tcgatgtaca
      961 atcaaaaaca atcagtttaa ccgaagcagg gatcgaaaaa gcagaagaaa cattcggttt
     1021 ggataactta tacgacattg aaaatactgc attgacccat cacttggatc aagcattgag
     1081 agccaactat atcatgcttt tagatatcga ttatgttgtc caagacggaa aagtactgat
     1141 tgttgaccag ttcactggtc ggatcatgga tggacgccgt tattcagatg gacttcacca
     1201 agcaatcgaa gctaaagaag gcgtagagat cgaagacgaa acgaaaacca tggcgacgat
     1261 cactttccag aactatttcc gtatgtacaa gaaacttgcg ggaatgactg gtacagctaa
     1321 aacagaagaa gaagaattca gagaaattta taacatccaa gtataccaga ttccgacaaa
     1381 tcgtccggtt atccgtgatg atcgtccaga tttgctttac ccaacattac aaagcaagtt
     1441 ccacgctgtc gtgcaagata tcaaagatcg ctaccgcaaa ggtcaacctg tgttagtcgg
     1501 aactgttgcg gttgaaacat ctgagttatt atcagatatg ctgaatcaag aacgtattcc
     1561 tcatgaagtg ctgaatgcga aaaatcactt taaagaagca gagatcatca tgaatgctgg
     1621 tcaaaaaggt gctgtgacga ttgctaccaa catggctggt cggggaacag atatcaagtt
     1681 aggacttggg gtaagagaac ttggtggtct agcagttatc gggacagaac gacatgaatc
     1741 tcgtcgtatc gataaccagc ttcgaggacg tgcaggacgt caaggagacc ctggtatgtc
     1801 acagttctac ttgtcattgg aagatgattt gatgaaacga ttcggttcag aacgaatcaa
     1861 atctttctta gatcggatga agatcggtga ggaagatgct gttatccaaa gcaagatgct
     1921 gacgaaacaa gtagaatctg cacaaaaacg tgtagaagga aataactatg acacacgtaa
     1981 aaacgtctta caatacgatg atgtgatgcg tgaacaacgt gaagtaatct ataaacagcg
     2041 acaagaagta atcatggaag aaaaaagctt atccaagcca ctaatgaaca tggtcaaacg
     2101 gacaatcagt cgtgtagtag atgcgcatac acaattagag aaagaaaatt ggaatttgga
     2161 aagtatcatc gattttgccg gcagtactct tgtccatgaa gatagtatta ctcttgcaga
     2221 tatcgaagga aaaacagctc aggagatcaa agagtatcta aacgaacgag cacaagaagt
     2281 atttaatacc aaagcggctc aactaaatgg tccagagcaa ctgttggaat ttgaaaaagt
     2341 cgtgatcttg cgtgttgtgg actctaaatg gacagaccat attgatgcaa tggatcaatt
     2401 acgtcagtca atcggcttac gtgcttatgg acagaacaat ccattagtcg aatatcaaac
     2461 agaaggctat aagatgtttg aagatatggt aggcgccatc gagtatgaag tcactcgaat
     2521 ctttatgaaa gctgagatcc gtcaaaacgt acagcgtgaa caggtttctc aaggaacagc
     2581 ttctcactca gaagacggag atgccaaaga aaatagtaat actagcgcga aaaaacagcc
     2641 aatccatgtg aacaaggtcg gaagaaatga tccttgtcct tgtggaagcg ggaaaaaata
     2701 taagaactgt catggaaaaa atgcaaacta atttctgaca gcaggattgg tgagacaaat
     2761 tcttttttta agaattcgtc tcaccttctt tctgttattt gctgattctg ttagactaga
     2821 tataatgaaa aaagtatagt gaaagaagga aaaaacatgg aaatatccga aattcgaaat
     2881 ctcctgacag aaatgcagga aaaggtcaca agcttcagga ggtctctttg acttagacca
     2941 gctggaagaa gacattgcgg aagcagaagc aaaaatggct gaacctggtt tttgggatga
     3001 tagcgaagct gctcaaaaag tgatcagtga aaacaatgca aataaagaaa cttatgatca
     3061 attccattct cttgctgaag aactggaaga aatcgaattg atggtggaga tgttgcagga
     3121 agaacctgat gcagccatgc aagaagaagc agaaacacgt atcaatgcgc tagatgaaaa
     3181 aatgaagaca tatgaattat ctatgttgtt agatggtcct tatgatcgaa acaacgccat
     3241 cgttgaattg catccagggg ctggcggaac ggagtcccaa gattggggca gtatgctttt
     3301 acgtatgtat acacgctggg cagagcagca tgggtatcaa gtagagacac tagattatca
     3361 agcaggtgat gaagcaggaa tcaagagtgt gaccctactg atcaaaggac acaacgcatt
     3421 tggttatctg aaatcagaaa aaggtgttca tcgccttgta cgtatctcgc cttttgactc
     3481 agcgaaacga cgtcatactt ctttttgttc ggtagatgtg atgccagagc ttgatgaaac
     3541 gatcgatata gaaatcaatc cagatgattt gaaaatcgat acgtaccgag caagtggtgc
     3601 aggtggtcag cacatcaata aaacagaatc tgctgtacgg atcactcact tgcctacagg
     3661 aacagtagta gctagtcagg cacaacgttc acaattgaaa aaccgtgagc aagcgatggg
     3721 gatgttaaaa gctaagcttt atcaattaga agtggaaaag aaagaacaag aagcagctgc
     3781 acttcgagga gaacaaaaag aaatcggctg gggttcacag atccgttctt atgttttcca
     3841 tccatattct atggtaaaag accatcggac aaacgtagag acaggaaatg tccaagcagt
     3901 tatggacggt gaccttgatt tatttattga cggctattta aaaatgcatc tacaataaga
     3961 acccattgtt aaattgaaat aaaattgtaa gattttgcaa gcatgttgaa acattgccat
     4021 gctataatag ctaaagactg aaaaagatat ggagagaacg ccatgattga aatgaaggat
     4081 gtaatgaaga agtactcaaa tggtacgacc gctatccgca atctctcaat tgtcatcgaa
     4141 caaggagagt ttgtctatgt ggttggtccg tctggagccg gtaaatctac attcatcaaa
     4201 ttgatgtatc gagaagaaaa agcttcaaaa ggaactttga acgtcgcagg tcatgatcta
     4261 atagcaatca aaaaccgcga agttccttat ctacgcagag aaatcggcgt ggtgttccaa
     4321 gactataaat tattaccaag aaaaacagta tatgaaaatg ttgcgtacgc tatgcaagtt
     4381 atcggcagaa aacctcggga tatcaaaaaa cgagtaatgg aagtgctgga ccttgttggt
     4441 ttgaaacaca aagttcgagt ctttcctagc gaattatccg gtggggaaca gcaacgtgtc
     4501 tctattgctc gggcaatagt gaatacgcct aaagtactta tcgcggatga accgacagga
     4561 aatctggatc cagaaaactc atgggaaatc atgaaattat tagatcggat caatgcccaa
     4621 ggaactactg tcgtgatggc tacacataac agtacgatcg taaatacgat tcggcaccga
     4681 gtaattgcta tcgaaaacgg ccgcattatt cgagatcaag cggaagggga atatggatac
     4741 gatgattaga acctttttcc gccatttatt agaaagtatc aagagtttga aacgtaacgg
     4801 ctggatgaca gttgcttcag tcagtgcggt aacgatcaca ttgactttag tcggtgtatt
     4861 catggcagtt atcatgaacg caactaaatt agctcaagat atagaaggaa acgttgatgt
     4921 ctctgtattc gtagatatcg gtacaaaaga cgaagagatg aaaaagctag aaaaagaatt
     4981 gaatgactta gatcatgtta aaaaagttga attttcaagc aaagaacagg aattgaaaaa
     5041 aatccaaaac gaaatgggtg attcctggaa attgtttgaa ggtgacaata acccgttata
     5101 tgatgtgtat gtagtaagtg ctactgatcc ttcttataca aaacaagtag cagaagaagc
     5161 agcagatttg aagaatgttt ctcgttctga ttacggtgga gcttcctcag atcggatttt
     5221 ccaaatcgct ggtgcagttc ggacatgggg attagttgca gcggcattgc tgctgttcgt
     5281 agcgatgttt ctcatttcga acacgattcg gatcacgatt ttgtctagac agcgtgaaat
     5341 tcaaatcatg cgtttagtcg gtgcaaaaaa tggatatatt cgctggccgt ttttccttga
     5401 aggtggctgg atcggtctga tcggtgcgat tcttccgatt ttgatcatca catttggtta
     5461 tgcatggttt ttccaattga tcaatccatc attattacgt tcccattatt cattgattca
     5521 tccacaaaat atcgtttgga aaatcaatct tttaatggtc ggcattggtg tgatcattgg
     5581 ttcacttggc tcgatcattt ctatgagacg tttcttgaaa ttctaatacc acaatataaa
     5641 taaaatgaga aagttggaca gtttcgttcc agctttcttt ttttattggc taggagaata
     5701 gaaggaagcg gcttttttcc ctttacttta aaaaacaact tgggtattct taagttggta
     5761 gaagaagagg tgaagccgtg aaaattatcg ttgacaattt ttttacgatc cttttgatta
     5821 tgaatatttt gctttccttt atcatcgttt ttcgagaaag aaaggaaact gctcaaacat
     5881 gggcatgggt gctcgttttg atgttcattc cagttgtagg atttatttta tatatttttt
     5941 taggaagagg gatttccaaa gagaaaatct ttgatttgaa gatccaaggg aaaatcggta
     6001 agaacctgga aattgaagaa gacaaacagg cattgatgcg agggctttat cctcatccgc
     6061 ctactggaca agtagatgtg aaacaattga tctatatgct cacggtattt gaaagtacgc
     6121 tctatacgac taaaaatgaa atcactttat ttacagatgg tcgtgaaaaa ttcgatgcgc
     6181 tgattgaaga tatcaagcag gcagaagacc atatccactt ccagtattat atttatcgca
     6241 gtgatgcatt aggtgaagaa gttcgagatg cattgacaga ggcggcaaga cgaggggtaa
     6301 aagtcagagt ccttcttgat gcatggggtt ccacacaagt ttcttccagc ttttttcaga
     6361 atttgaaaaa agcaggcgga gaagtcgcat ttttctttcc tctatttgtt ccgtatatta
     6421 atcctcggat caattatcgc aatcatcgta aaatcgtagt tattgatggg aaaatcggat
     6481 atacaggtgg gttcaatgtt ggaaatgaat atctaggatt ggtaaagaaa tttggttatt
     6541 ggcgagacaa tcatttgcgt atttacggag aagcggtgta taccttgcaa aaccgttttt
     6601 tgatggactg gaattctcag catacaaaag aagtaggcta ttctcccaaa ttttttccgt
     6661 ccattcactc tactggtgat atagctgttc aaatcgtgac aagcggacca gataccgaac
     6721 atgaacaaat caaaatgact tatttgaaaa tgatttcgtt agccaaacgg gagatattga
     6781 tccaaacgcc ttattacata ccggatggat cgattcatga agcactgaag ttggctcttc
     6841 tttcaggcgt aaaagttcat atccaaatac ctaacaaacc agatcatctt ctcgtttact
     6901 gggcaacgta ttctttcgct gcagagctaa tcgagtatgg agcaaggatc gaaacatatg
     6961 agaatggatt tattcatgca aagacgatga ttatcgatgg tggcattgtt tcagttggct
     7021 cagcaaatat cgatgttcgt tcatttcgct tggactttga ggtcaataca ctggtttatg
     7081 atgaacgaat ggcttcacgt gtaagaaaag ctttttttga agactcgaaa atttctacgc
     7141 atctcacaaa agaaatgtat gaaaaccgag gaataatcat caagatgaaa gaaggattag
     7201 caagattgat ttcgccgctc ctttaaaaca taaaaaggac atgagaacca tcaacagatg
     7261 gagatctcgt gtccttttct aacggggcca tttctgagag tacgtcagaa atggagaaag
     7321 cgtctcgcat gagcgaaatt aactttgggg tatctcttgt tttttgggga aagcaagaaa
     7381 taagctaata tatgctatta aaagagacac atttcaaagc cagtatagca tagaaacttt
     7441 taatgagata gtttttttta caattatgag aatttatttt gttatttaat gattttgatt
     7501 cgttttcatc aagtaatgaa aatgataaaa taaacatact ttaggattaa aggagatttc
     7561 taatgaaaaa acttaaatta ttcttaccaa taataggatt agctattctt ttatcaggct
     7621 gttcaaagtg gattgatcga ggagaatcca ttacggctgt tggctcttct gctttacagc
     7681 ctttagtaga aacagttgct gaaacttacc agacagaaaa tcctggaaaa ttcatcaatg
     7741 tacaaggggg cggaagcgga actggtttgt ctcaagtaca gtcaggagca gtcgacatcg
     7801 gaaactctga tttgtttgcg gaagaaaaat cggggatcga tgcctcagca ttagtcgatc
     7861 atcgagtagc agttgttggg atcactccta ttgtgaacaa ggatattggc gtaaaagaca
     7921 tcagtatgga aaacctgaaa aaactattta caggcgagat caaaaactgg agtgaagtcg
     7981 gtggaaaaga tcagccagta gttatattga accgcgcttc aggaagcgga acccgtagca
     8041 cgtttgaaaa atgggtattg gacggagaaa cagctgttca ggcacaagaa caagattcaa
     8101 gcggaatggt gcgccagatc gttgccgaca cacccggtgc aatcagctat gttgcttttt
     8161 cttatgtttc cgatgatgta gatacgttga atgttaacgg tgtagaaccg acagatgaaa
     8221 atgtgacaac gaacaaatgg acgatttggg catatgagca tatgtacaca agagatcatc
     8281 caaaaaaact gacaaaagaa tttttagact atatcttatc tgatgaagta caagataaca
     8341 tcgttggaga actaggttat atccctgttt ctcatatgaa agtcgaacgt gattggcaag
     8401 gaaatatcgt taaagagtaa tctttacaca aaatttacaa acttagctaa acttttacat
     8461 gttattaaaa ccaaattgat gttcaatttg tacactgagt gagtatgtta aacctgatta
     8521 agggggagtc atctgtggaa gatgtgaaaa aagtattatt aacaaaatca aaacgttcaa
     8581 aaatggaaca gcgtggaaaa ttcatcagtt ttttatgtat cgcgctgatc gtgttggttg
     8641 tattatctat tttttatttt gtagctagta aaggattggc cacctttttt actgataaga
     8701 tcaatgtatt tgattttctg tttggtacag attggaatcc aagccaaatc ggagcaaatg
     8761 ggaaaccaat ggtaggggca ttgcctatga tcacaggttc gtttattgtg acctttttat
     8821 ctgctatcgt tgccacgcca tttgccattg gtgcagcagt attcatgacg gagatctctc
     8881 ctaaaaacgg aacgaagatc ttacagccgg tcatcgagct gcttgtaggt attccttctg
     8941 tcgtttatgg ttttatcggt ttatcagtta tcgtgccttt tgtcagaagt atttttggcg
     9001 ggactgggtt tggtatctta gctggtacat ttgtgttgtt cgttatgatc ttgccgactg
     9061 tcacaacgat gactgttgat gcattaaagg cagtgccacg gcactatcgg gaagcttcat
     9121 tagcgcttgg tgccacaaga tggcaaacaa tctacaaagt cgtgctacga gcagccgtgc
     9181 ccgggatttt aacagcagtc gtttttggta tggctcgtgc gtttggtgaa gccttagcta
     9241 tccaaatggt catcggtaat gcagcattga tgccaacagg tcttactact cctgcttcta
     9301 cattgacgtc catcttaaca atgggaatcg gaaatacaat catgggaaca gttgaaaata
     9361 atgtgttatg gtcattagcg ctgatcttgc tattgatgtc attattcttt aatatcgtga
     9421 tccgtatgat tgggaagaaa ggagagctga aataatgaac gcaaaacgct cagataaaat
     9481 tgctacaggt gttttatata tagtttcggg gatcatcgta ttgattttag cagctttact
     9541 actttatatc ttagttcggg gaataccgca catttcatgg tccttcttga ccgaaccttc
     9601 caaagcctac caagaaggcg gtggaatcgg gatccagcta tttaactcga tttatttgct
     9661 tttgatcacg atgatcatca gttttcctat ttctttaggt gcgggtatct atctttcaga
     9721 atatgctgga aaaaactggc taacagatgt gattcgcacg tcgattgaaa tcttgagctc
     9781 gttgccttct gtcgttgtcg gtttattcgg tttcttggtt ttcgttattc aattccagta
     9841 tggtttctcg attatttctg gtgcattggc tttgactgta ttcaacttgc ctcttttgac
     9901 tagaacagtt gaggagtctt tacaagcggt ccattacaca caacgagaag caggattggc
     9961 cttaggtttg tcacgctggg aaacagtgat aaaagtggtg gttccagaag cgacaccagg
    10021 gattttgact ggtgtgatcc taagttctgg aagaattttt ggtgaagcag ctgcattgat
    10081 ctatacggct ggacaaagtg cacctgcgtt agattttacg aactggaatc cattaagtgt
    10141 ttcaagcccg atcagcattt tccgtcaagc tgaaacatta gctgtccata tttggaagat
    10201 caatacagaa ggcacgatgc ctgatggtgt ttctgtttcg gctggcgctt ctgccgtgtt
    10261 gattattgct gttttactgt tcaacttcgg cgctcgtgcg attggcaaac gattataccg
    10321 aaaaatgact tcagcctaag gagaagaagc aaaatgaaaa aatataactg gaatgaaaca
    10381 aatgtcatca ccatgccaaa agagaaagaa gtagcactat atacggatga tctacatgtc
    10441 tggtatggcg acaatgaagc aatcaaagga gtggatctgg aatttgaaaa aaataagatc
    10501 actgcactga ttggtccttc cggctgcgga aaatctacgt atcttcgttc gttgaatcga
    10561 atgaatgatg ggatcgctaa tacgaaaatc actgggaaaa tcatgtatca aggcgtagat
    10621 attaatgcgc ctcaagtgga tgtttatgag atgcgcaaac ggattggcat ggtttttcaa
    10681 cgtcctaatc cgttcagcaa gtcgatttat gacaatatta catttgcttt gaaacagcac
    10741 ggcgaaaaaa acaaaaagaa attagatgaa atcgttgaaa caagcttgaa acaagctgcc
    10801 ttgtgggacc aagtaaaaga cagcttggac aaaagtgcct tggctttatc tggcgggcaa
    10861 cagcaacgtt tatgtattgc tcgtgcgatc gcgatgaaac ctgatatctt gctgctagac
    10921 gagccggcaa gtgctttgga ccctatttcg acaggaacgg ttgaagaaac acttgtaaac
    10981 ttgaaagaag actatacgat catcatcgtg acgcataaca tgcaacaagc agctcgtatc
    11041 agcgactata ctgccttttt ctatttagga aaagtaatgg aatatgacaa aactaacaag
    11101 atatttacca gaccaaaaat tcaggcaacc gaagattatg tgtccggtca ttttggttaa
    11161 gacggaggcc tcaacatgac aaaagagatt atttcatcta aagatcttca tctgtattat
    11221 ggaaagaaag aagcactaaa agggatcgat ctaagcttca atcaagggga aatcactgcg
    11281 atgatcggtc cttctggttg cggaaaatcc acttatctgc gttctttgaa ccggatgaat
    11341 gacctgattc cgaatgtaac gatcaccgga agtgttttat ataaagacaa agacatctat
    11401 ggtccaaaaa cagatgttgt tgaactaaga aaagaaatcg gcatggtatt ccagcagcca
    11461 aacccatttc ctttttctat ctatgaaaat gtcatctatg gattgaaatt aaaaggggaa
    11521 aaagataaaa aagtccttga tcaagtagtt gaagaaagcc tgaaggcagc gtctgtttgg
    11581 gatgatgtga aagacaaatt gcataaaagt gctttatctt tatccggtgg gcagcagcag
    11641 cgtgtatgta tcgcacgtgt tttggcagtt aatccggaaa tcatcttatt ggatgaacca
    11701 acaagtgcgc ttgatcctgt ttctactggg aaaatcgaaa gtatgctgtt agaattaaga
    11761 gagcggtata cgatgatcat cgtgacccat aatatgtctc aagcttcacg tatttcagat
    11821 aagacggcat tctttttaga tggtcacttg atcgaataca atgatacgaa gaagatattc
    11881 atgaatccag aaaaacaaga gacagaagat tatatttcag gtcgatttgg ataaggagga
    11941 aacggaatgc tacgtacaca atttgaagaa gaattgctga atctccataa tcagttctac
    12001 gaaatgggga tgatggtgag cagcgccgtg cacaagtcag ttcgtgcata catcaaacac
    12061 gataaaatct tagcccaaga agttatcgaa aacgatataa atatcaataa catggaaaca
    12121 cgcttggaga agaaaagttt tgaaatgatc gcattgcaac agccagttac aactgatttg
    12181 cgtatgatca ttacggtcat gaaagctagc tcggatttag aacgtatggc cgatcatgcg
    12241 gtgtctgttg ctaaatccac gattcgcgta aaagggcaga cgagaatacc ggaaatcgaa
    12301 aaagagatct ccgatatgtc cgattatgtg aaaaaaatgg tggacaacgt cctagttgct
    12361 tatgtcaaaa cagacgaaaa agacgcacgt acgatcgcca atatggatca aagagtcaat
    12421 gactatttca atcgaatcta taatgcaaca atcaaaaata tgcaggaaaa tcctgagaca
    12481 gtcatcagtg gaacagacta cttgaatgtt gccagttatc tagaacggat cggagactat
    12541 gtgacaaaca tctgtgaatg gatcgtctac ttggctacag ggaaaatcac tgagttaaat
    12601 acgaaccaca atgagatttt ctaaactgaa gctgtgatta tgccgtttgt ttaggaaaag
    12661 aggttgtgaa atcagcggtt gtgaaaaagc tgttctgcat caagtaataa gaacagtcaa
    12721 acaaaaaata gctcctcata tttttcgttt ggctggactt attaccgcag atgcaagctt
    12781 ttgaacaccg tttattcggg aaagggtttg tgacaaaaca tgtcacaaac ccttttcatc
    12841 atttcataga aaggataaag aatgcaaaaa aaaattagca tcatcactat attttttatt
    12901 tgtgtgctta cttcatgggt tattcagcat caagccgctt tatatgaagt acctgtcatc
    12961 gagatagaaa aagtacgaat aaaagaggat catcaattga ttacaggaaa actgatgaac
    13021 acggatcaaa aaggagaatc gatttattta tcggttcctt atcaaaaaaa tcaaatagaa
    13081 acagcaaata taaaagcgag acaaatgtat ctagtccaaa tgcatggaga tgaatggcag
    13141 ctagtagcaa aaaaaaatga tggctggctt ttcttcttca caagtatctt cattggcgcc
    13201 atgttgctta ttggtggaaa acagggtcta tttgctttag gaagtctcat catcaatatc
    13261 gttttacttt tacttttgtt gctcatcttt actaaaacag aagggatttc tctattaaat
    13321 atatctatcc tgttcgtcgt tatagggatg attattagca tattggcttt agatggatgg
    13381 aatagaagca gtataatcaa aatttcagct gcagttacga gtgttttttt agcatttttt
    13441 atttgtcttt tgacaatgaa tcattggaaa gaccaaggac tgcgctatga ggaactgaat
    13501 tatctgacac gaccgtatcg ttctgttttt ctttctagcg tactgattgg aacagctgga
    13561 ggaacactcg ataccgttat cacagtcatt gcgacattag aagaattgac taagcgacaa
    13621 ccagaaataa cagcgaaaca gttatggctt tcaggaaggc aagtgggtcg agatgtgatt
    13681 ggctcgatga ccaatatcct tttgttcgtc tatatcagtg ggtcagttcc atcgcttctt
    13741 ttatatttag gaaatcaatg gtcgttcaaa gaaacgatag aacagcattt atcattggaa
    13801 tttttacgcg tcattgctgg cagcttagga atagtattat cgatccctat ctcggtcctc
    13861 ttttttctga ctgtaaggag gctgaaacga tgaatgtact tattatactt gttgggattt
    13921 tagcaatcag cgtgctactt attggcggta cgcaaggtat gtatattctt ttaggcttat
    13981 tcatcaattt agggatcttt ttcctgttgt tattcggtta tcatcagaag tggccgatac
    14041 tggcattgtc ggtcatagga tttttattga ttgctgtggt tatcttattt tttatcaacg
    14101 gttataatct aaaaatgcgt gcagcttttg tgagtatact gatcttttta ttttgtttcc
    14161 tactattgct tcctatcact gattttttag caatccaagg ttttacatcg gtagaactag
    14221 aagaattatc tggcttagat aagactttag cgattgattt tagattactt acgagatcgt
    14281 tgcttctgat tagtcttagt ggtgctgttt tagatgcgag tgtagcaatt tctagtggga
    14341 cattcgaagt atatcaggca aatccgcaat tatcttttta ccaattgcgc cattctagtt
    14401 tcgctattgc taaaaaaata ttggcgtcaa cagtaatgac acttcttttt gcattcatgg
    14461 gaagttcttt agcgttgatc ctctggttta ttgatttaga cattcctttt cagcaagtga
    14521 tcaatgaaaa atcatttgtt ctagaatata cgatggctat tcttaccaca ttgtccgcac
    14581 tacttgttct tcctttaacc tgcgtgacta cagcatatct ctttacaaaa aagaaagaac
    14641 aactcaagat ggagtaaaag ttacaactta agtcctatga catttttact gaatcagtgc
    14701 ataatatgga tgtaaggaaa agcttgcaaa caattttgct taaggaggca atttccatga
    14761 aagaaagaga acgtatttta gatttagtga aaaaaggaat cctttcgact gaagaaggct
    14821 tagatttatt agaaagcatg gcaactgaaa aagacgaaaa gcaaatcaaa aaagaagcag
    14881 acaaggtaac tgctagtcat aaagaaaaag atcaggcaag tcaattgatc gataaacttg
    14941 aaaacggcga agaagaaatt tctgagccag ttgatccaaa ggaaagagaa agacaagatc
    15001 aagaaaactt agaaaagatc ttagatgaac tagcaactga agcgaataaa acttcggcgc
    15061 gtctagatga agtaaatgct caattcgctg acaataaggc agcacgcaat gagaaacagg
    15121 aagcattgat gcagctgaat acaaaagaag aattaggcga gttgacagaa gaagaattag
    15181 cacagcgtca gacgttagaa gcagaaatca aagaattaga agctagtggc gatacactgt
    15241 tagaagaaca attgaaatta gaagcagaat taaaagatat ccgtaagaat caatggtctg
    15301 agaaaaaaga aacgtttact gataagtttg aattgccaga tgattggaaa gatcaagcaa
    15361 cagatacact gaatcaagta ggcgaaaaaa tgtcagaagc tggatcacag ttaggcaaat
    15421 tcttgaagaa aacattccaa actgtttcag aaacagtgaa tgacaacatg gaatggaaag
    15481 atgtcagctt acgtgtccct gggattgcga caacgaaatt tgagcatgaa ttttactatg
    15541 aagctcctgc agcaagtatc cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa
    15601 cgtgggacag caacgatgtg aaagttgaag ctaagatcaa actttacgga aaaatgggtg
    15661 ctgaaccatt tgaagcattc tctgaacgga gccagattga ggttaatgaa gatcatatct
    15721 ccttccaaat tccaaataaa cgtgtgcgtg cagaccttgt tttctactta cctaaacgag
    15781 tatacgacca cgcagctatt aaacttctaa atggcaacat catgattgaa acattggaag
    15841 ctaaagatat ttatacaaaa tctacgaatg gtaacatcat cgtggaccaa ttaactgcaa
    15901 cgatgctgga agtagaaggt gtaaacggca atatcgatat tcgaaacggg aacatcttag
    15961 attctattat cgaaacagta aatggtacag tgacatttgg tgcgacagca gaaaaccttt
    16021 ctgtgtcttt ggtaaatggt gatgtacgtc ttacgattaa agaagataat ttgaaaaaag
    16081 tagaagcaag ttcagtgaac ggaaatgtca aagtcgcttt accagatggc atcggtttgg
    16141 aaggacatgc taaaacaagt cttggaagta tcaacagccg tctgtccaat tacgaagtag
    16201 ttcgtgagaa aaaagaacgg acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatggtg
    16261 aaatcgcgca agtgcaactt tcaacgacaa caggaagtat ctatttaaaa gatacagata
    16321 aataaagagg ttgtgaaaga gatgttcagc cttgagcaat aagaagcgat tttgtaaaat
    16381 agcttcccct attttacaaa attgtgcctt attgccgata gtctatctct tgaacaccgt
    16441 ttaccaagag attatgaacc tgccaattat cgccgagtca caaggaataa tttcagaaaa
    16501 agctcgtcac gttttatgaa tatggtaatt tgtgaccgag aattttgcag aaagacaccg
    16561 attaagaaga aggttgtcac aacgattttc ttcatttaaa atagtagaac agaggtgaga
    16621 catgatgaaa aaaaagttga caaaatctaa taaaaatgtt gttttgacag ggactttagc
    16681 aggaatagga gaatacatcg ggattgatcc gactgttatc cgtgtgcttt atgtattcct
    16741 gagcttagtt gcttttggaa gtccaattat tttatacatt ttattagctt tgattatccc
    16801 atctgcgcgt tctgcacgaa caaactatgg acataacaat gcatattatg aaaaaaacaa
    16861 ttatcgtgaa caaactcgtg aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga
    16921 tgacgaatgg agtgactttt aatgtcgtac tttcaacgta ttttagttaa tacgttaaca
    16981 ttcatatcat tggctgttct ttttcctgcg atggtccatg tgagcagtat ttggacggcc
    17041 gttcttgcca gcttcgtatt gtctgtgttg aatatgtttg tcaaaccgat tttgacgata
    17101 ctgtccctgc cctttacatt agtcacattg ggattgttta cgtttgttat caatgcagcg
    17161 attcttaaaa tgacctcctt ttttgtggga gcagaacatt ttagtttttc ttcattcggt
    17221 gcagcgttgt tagtagctat cttgatgtcg cttgtcaatg taattgtcag cgaacataac
    17281 agtagaaaat aaaagaaagc tgtgccgaac tatcggcgca gtttttttat ttagaatcag
    17341 gaatgcgttt tctattacat tggtaattaa ataaaaacgt tttttttaat tggtagtact
    17401 tttggctaga caatgataaa atgatagaga acactctaat agaaatgagg tagcatgaat
    17461 gtcagaagtt gtaaaagtta gccaattagt gaaaaaatta tctttggaaa tcgtgacagg
    17521 cgacgagaaa agcttagacc gtgtcatcgt aactggggac atctcacgcc caggactaga
    17581 gttgactggt tattttaatt attattccca taatcgttta cagctatttg gcagtaaaga
    17641 aatcagtttt gcggaacgga tgatcccaga agagcgtttg atcatcatga gaagattatg
    17701 tagtgaagat atgcccgcat ttattatttc aagaggatta ccagctccag aagaaatgat
    17761 ccaggcagca aatgaaaatg ggatagctgt actgcgttct cccatctcaa cttctcgttt
    17821 attaggagaa ttatccagtt atttagatgg tcgccttgcc cctcgaacaa gtgtccacgg
    17881 tgttttagtg gatgtttacg gattaggtgt actgattcaa ggtgacagtg ggataggaaa
    17941 aagtgaaaca gcgttggagt tgatcaaacg cggccatcga ttgatcgcag acgatcgagt
    18001 agatgtttat cagcaagatg aattaacggt gatcggagaa ccgcctaaaa tcctggaaca
    18061 tctgattgaa atccgaggaa tcgggatcat tgatgtaatg aatctttttg gtgcgagtgc
    18121 ggtacgtggc tctatgcaag tccagttggc tgtttatctg gaagcatggg caaaagataa
    18181 gaaatacgac cgtctgggct ctgaagacac gaccgttgaa atcgcggaag tgggcattcc
    18241 acaaataaaa atccctgtca aaacaggacg aaacgttgca atcatcattg aagtagccgc
    18301 gatgaatttc cgcgcacgta caatgggctt tgacgcaacg aaaacatttg aagaaagatt
    18361 aagtcgcttg attgaagaaa actcaggcaa cgactagcaa ggagtgacaa tatgttagca
    18421 caaatcaatc gagttgcttt tgagcttgta gggattccta tctactggta tgcattgatc
    18481 atcgtatcag gtatcattat cgctatgtgg cttagcagca gagaggctgt tcgggtagga
    18541 ttaaaagcag atgatgtgac agattttatg ttgtggggat tgcctatctc cattattggc
    18601 gctcgcttat attatgtttt atttgatctt ccccagtata tagctgatcc aatccaaata
    18661 ttcaatatcc gttcaggagg cttagctatt tatggcggac tgattgcagg cgcgatcacg
    18721 ctgtattttt ttactaaata tcattttatt tctttgtgga cttttttaga tatcgctgca
    18781 ccaagtgtat tattagcaca ggcaatcggt cgttggggaa atttcatgaa ccacgaagca
    18841 tatggaccag acacgacgcg aagcttctta gaaagtctgc atttaccgca attcatcatt
    18901 gataatatgt atatcgaagg gatctatcgt caaccaacat ttctttatga atcactttgg
    18961 agtttgattg gatttatttt gcttttgttt ttacgaaaga aaccacacct tttgaaaaaa
    19021 ggcgaagtcc ttttatgtta tctaatgtgg tatagtttcg ggcgtttctt tattgaagga
    19081 ttacgtacag acagtcttta ctttttaggt gtgatccgta tttctcaatt attatctgct
    19141 gtgttatttg tcggtacatt gattctattt atttggcgca gaaaaaaagg aaatttaccg
    19201 gattatgacc gctcattagg aaaaaatcaa caaattatct gatttttaga gcgttaaaaa
    19261 tgaaaaaaat ttcaggagtg gtaaaatgaa ttatagagta gcggttttag gaccaggctc
    19321 ttggggtact gctttggcac aagtcctcgc cgaaaacgga catgacgttc ggatttgggg
    19381 acatcgtgct tcacaagtag aagaaatcaa cacccaacac acgaatcaac gttatttacc
    19441 agatcattat ctacccgatt ccatccaagc attcaccaat atggaagatg cagtaaaaga
    19501 cgcagatgcg gtgctattcg tcattccaac gaaagcaata cgttctgttg caaaagaact
    19561 agtgcctaaa ttgaatacaa agccagtgat catccatgca agtaaaggat tagaacaggg
    19621 aacacataaa aggatctctg acatcttgtt agaggaaatc ccagcagaaa aaagaaaagc
    19681 tgttgttgtt ctttctggac caagccatgc agaagaagta gcaaaacatg acatcactac
    19741 gatcacagct gcaagtacag atgcaaaagc agcaaaatat gtacaagagc tgttcatgaa
    19801 taattatttt cggatctata ccaatcctga tgtgatcggt gtagagatgg gggcagcttt
    19861 gaaaaatatt attgctattg gtgctggcgc tattcacggc ttagggtttg gcgataatgc
    19921 aaaggcagca atcatgacgc gcggattagc tgagatcagc cgtttaggtg tagcaatggg
    19981 tgccaacccg ctgaccttta tcggattaag cggaataggc gatctagtcg ttacttgcac
    20041 aagtgttcat tctaggaact ggcgcgcagg caacttgtta ggacaagggc agcccctaga
    20101 agaggtgcta gccaatatgg gaatggtcgt tgaaggtgtg aatactaccc aagcagcgat
    20161 ggaactagcc caaatgatgg aaatcgaaat gcccatcact caaacaatct atgaagtgct
    20221 gtatgaacaa aaagaaatca aaacagccgc aaaagaaatc atgttacgtg atggaaaaaa
    20281 agaaaatgaa ttctcattaa caattttttg aaggagcgaa tgtatatgaa agttaaaaaa
    20341 gcagttattc cagcagcagg attaggaaca agatttttac cagcaacaaa agctatggca
    20401 aaggaaatgt tgccaattgt tgataaacca acaatccagt tcatcgttga agaagcgttg
    20461 aaatcaggta tcgaagacat cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat
    20521 cactttgatt caaatattga attagaaaca aacctaagtg aaaaaggaaa gaccgaatta
    20581 ttgaaattag tagaagaaac gacagatgtc aacttgcatt tcatccgtca atctcatcca
    20641 aagggtttag gacatgccgt tcttcaagca agagccttta ttggaaatga accatttgtc
    20701 gttatgctag gcgatgatat aatggaagac gaagtaccat tgacacagca attaatcgat
    20761 gattacgaac gtactcatgc ttctacgatc gccgttatgc aagttcctca tgatgaaact
    20821 tcaaaatacg gaattatcaa tccaggagaa gtattggaag atgggttata caatgtcaaa
    20881 aactttgttg aaaaaccaga tccaagcaaa gcaccaagtg atttagctat tattggacgt
    20941 tacttattga ctccagaaat ttttgatgta ttgtccgagc aacaaccagg agccggaaat
    21001 gaaatccaac taactgacgc aatcgataca ttgaataaaa cacaacgcgt atttgcccgt
    21061 aaattcacag gtaaacgtta cgatgtcggt gacaaatttg gttttatgaa aacaagtatt
    21121 gaatatggtc taacacatcc agaagtaggt gcacctttac gtgagtacat cctgtcttta
    21181 ggtaaaacat tagaagcaga aggaagcaag atcgatcata atccaaaagg aataacagaa
    21241 atcaaagaag aaaagaaaaa ataacccttt tttctaacga gcttcagctg ctaattgaat
    21301 aaaaagtagt tgaagctcgt ttttatttgt ttaaaaaaca cgaaaatgct atccttatag
    21361 aaaagagaga caagaaggga gtttgatcat gtcaggagga gaaattgcag gattgatcgc
    21421 agcaatcgcc tttgcgatac ttgtcgtttt tatcgttcgt gtgttgttgc aagtttctaa
    21481 aactttagaa aaagtagatc agactgtagc agaagcaaat acgacgatcg aaatcgtcac
    21541 aaaagacgta gatcttttgt ctagacaagt agaaggctta ctggtcaaaa gtaatgaact
    21601 gctcttggat atcaatggaa aagtcgcaac gatcgatcca ttgttcacag cagttgctga
    21661 tttaagtgaa agtgtatcag aacttaatca ttctagtaaa aacatcgcga cgaaagtcgg
    21721 cggaatcggt aaaacgacag ctaaagcgac ggttgccgga aaagtcggtg ggacagcgat
    21781 gaaatttttt aaaaacaaaa aacatactga aacgacagga ggaaaataaa aatggctaaa
    21841 aaaggtggat ttttattagg tgcagtagtc ggaggcacag ccgcagcaat cgccgcgtta
    21901 ttgttagcac caaaatctgg taaagaactt cgggatgaat tagctgccca agcggatgat
    21961 ttgatggata tggcttcaga ttacacagat atggcgaaag aaaaatcgac tgagttaaca
    22021 ggtattgcaa aagaaaaagc gcaagatctt tctcaacaag caggtgatct agcaggtagt
    22081 gtcaaacaaa aagtaagtga atcagcagat gaagcaggaa gtgaatctga cgatatcttg
    22141 ttaaatctta aagaacaatc agctgactta tctaatcgtt tcaaaaaaac ggcagaagat
    22201 atgaaaaaac ctgcagatga attaggaagt attgctaaag atacttcaga agatatcatg
    22261 attgatgtaa aagacgctgc tggtaaagca ggagatactg tcaatgaagg aatgtcagaa
    22321 gcaaaaccag ttgcagatga ctcaaaagaa atgatgaaaa tggcaaaaga agatgcgaaa
    22381 gaagctaaaa aagaaataaa agatacaatg acagattcat ctacaaatca ctaaaatcac
    22441 aaatttatag aataaaaagg tgggagcgga ctagctctca ccttttttac tcttattttt
    22501 ctaaaacaag taattctttt ggtgtagtcg tgaatggcac acagccatcg cttgtcacat
    22561 atacacagtc ttcgatacgt acccctactt tttcaggaat atagattcct ggttcaagag
    22621 agaagcacat cccttcttcg attactaagt catttccttc tacaagggaa gggaattcgt
    22681 ggacggttgt tccgatcccg tgacctaaac ggtgattgaa atattcgccg tagccatagc
    22741 tgttgatcac gtttcgtgcg atttgatcaa gttcacctgc tgtcacacct ggacggacgg
    22801 cttcttgtgc ggcaagctgt gcttcaagag tgatgttgta gattttttct tggaagtcgg
    22861 ttggtttttg ataagcaatt gtacgagtag catcactgca atagccattc caaacaacgc
    22921 caagatcgaa taacacaagt tcattcggtg aaatcttcgt atttcctggt gtaccatgag
    22981 ggcttgcagc atttgctcct gcaaggacta atgtgtcgaa agacatttgt gaaacacctt
    23041 gttttttcaa ttggtattca atttctgcaa tgatcgcttg ttcagtcacg ccttctttca
    23101 ctgctttaaa gccaatctca aatgcaacat cggcccattt accagcttcg atcagtttgt
    23161 ctttttcctc tagagttttg atcagttgta gacgttggat gatggaagtt agatcagtgt
    23221 aagaatccgt tcccgtcaag atatcagaga tagcatcgaa gcgctcgaca gtcaaggcgt
    23281 tcttttcaat ccctacattg ccaggctgac catagcgagc acgaagttct tttccgatga
    23341 tctcccaagg gttttcgctg tctaaatagc cacggacatc atacgcccaa ctactgtttt
    23401 gagcatcttc aacttccaaa gcaggagtga aaaggaaagg atcttgttca agagggataa
    23461 aaagagctag tacacgctca tgaggatcac tttcgtatcc ggaaaagtaa gcgatgtggc
    23521 cagggtcgct gatataagcc aattcgactt ggtgttgtgc catccattga cgtagttcat
    23581 tgatttttac gttattcatt tggcaaccta ctttctaaat taattttgta ttcaataatt
    23641 gtaccactga tttattaaaa gaccaacgtt ctaaaaactg aaaaaagtaa aaattttcat
    23701 gaaaacagag ataaaacggt tgcaattctt gagattattt gatattctat tttagaaatg
    23761 taagacgtat tttcagaaaa catataaaat atgaatagga gaacacaaat ggaaaagcaa
    23821 acaatcacta tctatgatgt agcgagagag gcaaatgttt cgatggctac tgtttcacgt
    23881 gtcgtaaatg gcaatccaaa cgttaaacca gcaacaagaa aaaaagtctt ggaagttatc
    23941 gatcgcttgg attatcgtcc aaacgcagtt gcgcgtggct tggcaagtaa gaaaacaaca
    24001 acagttggtg taatcatccc tgatgtcagc aatatgttct ttgcttcttt agcacgtggt
    24061 atcgatgatg tggcaacaat gtacaaatac aatattatct tagcaaattc agatggcaac
    24121 gaccaaaaag aagtcaatgt attgaataac ttactggcta agcaagtgga cggcgtgatc
    24181 ttcatgggtc accatatcac agacgaaatc cgtggagaat tttcacgttc aaaaactcct
    24241 gttgtattag ctggctctat tgatccagat gaacaagtag gtagcgtaaa cattgattat
    24301 gtatccgcaa caaaagatgc agtaaacaaa ttggcaaaaa gcggcaataa aaaaatcgct
    24361 tttgtcagtg gtgcgttgat cgatcccatc aatggacaaa atcgtctgaa aggctataaa
    24421 gaagcattaa gcgaaaataa cctaccatat agcgaaggat tgatctttga agcacaatac
    24481 aacttcaaag atggtctatc tctagtcgat cggatccata atagcggtgc aacagctgtc
    24541 tatgtttcag atgatgaatt agccatcggt attttggatg gtttattaga ccgtggcgtg
    24601 aaagtaccgg aagaattcga aatcatcaca agtaacaatt cactactgac agaagtagct
    24661 cgtcctcgtt taacaagtat cacacaacca ttatacgatt taggggcggt atctatgcgc
    24721 ctattgacga aaatgatgaa taaagaagaa gtcgaagaaa aaacgattat cttgccttat
    24781 ggaatcgaag aaaaaggatc tacaaaataa taaaaggaca accattcaga ggttgtgtgc
    24841 ctgccgttta actctgcgtt acaaggagca atttcaaaaa acagcatctc atgtttgatg
    24901 aatattgtga ctcgtgattc ggagttggca ggagaacacc gtttatttgg gaaaaggggt
    24961 tatgacaaaa ttttgttata atcctttttt tgtgtcttaa aaaagagaga aagtggttga
    25021 aattacattt atcgtatata aaacaatccg tgtatagatg ctgcattttg ttcaccacca
    25081 tcaaaaaaat aagggactgt gacaatacta ttgtcacaat cccttattac tgtgtattct
    25141 attaaatcaa tcatctttct catctgattc tttagttgct tgattcgctc ttgcataagc
    25201 tgaagcggtg ttccagtaat ctgtatagtt ttcatcggta ccgccaacgc caaatttaaa
    25261 ggcgcttttc ttcggtccat ttttcgccca tagcgattca actgtttttc ctggtgtttg
    25321 gatggatttt ttattgacag taacttttcc aggtgtttga ccagtaaatc cagatacttt
    25381 tttcttcatc acatcagatg ataattcgaa tttctcatca gtgccaaaaa tgtcaggttt
    25441 tgcttggtaa gcttgattga tcagattggc aagataatta gccgaacgta tccttgcttt
    25501 gctgtccatt ccagtattgt catcgtgccc tgcccagcta ctgatggtaa tagacggagt
    25561 agagacaatt agccatgaat ctcggtattc attggtagaa ccagtttttc cgacccaatc
    25621 tgcttttcct agatttccgt ttaagcttga gatggcatct ttgaatggtg tagtgatttt
    25681 tgcattgatc acactacgca tcatgtcatt catgatcgat gccgttgctt ccgaatagat
    25741 ccggacagag ttgctttcgt gtttataaat tacatttcct gcgttatctg tgatggaatc
    25801 gatgatatag ccttcttcat agacgccgcc gtttgctaaa gcctgaaatc cgttggtctg
    25861 ttgaagcgtc gtgacatcta ctgtacctaa aggagcagat tcaacgcccc aattgtcatt
    25921 tgccgggtag ttcatctttg ccaagtagtg ctcgtaagca tattgtttcg atccaccgga
    25981 attcaacaca tcttgatata agtgataagc cgggatattg ttggaccatt ctagtgactc
    26041 acggacggtt tggaaggtgt tcgatccttc gttagtagca ttgacgattt tctcgccagc
    26101 attttctcct tcttgccaag ttgttgcata atctgataca cgggattctg atccgataag
    26161 ccccatgtcg attgcaggtc cataaacaag aactggtttg attgatgagc cagcttgacg
    26221 ttctgtatcg aaggcatggt tattttgatt ctgactgtaa ttccgtccgc caacgaatcc
    26281 gtagatgcgg ccagttctgt tatccatcaa tacatttccg gtttctactt gactgccagc
    26341 accatcatct aacatgtatc cataattttc aacgccattt tgcaatgcag catagacatc
    26401 tttatcgatt gtcgaatgga ttgtatatcc tttattttgg atcgtttgct gtgccttttg
    26461 ataataagcg tcactgaccg atgaatcaga catatcagcg ttgtcttttt cagccaattg
    26521 ttgtgcaatg attcttgttg cttcattcat cacagtataa tacaagaatt ctctgtcagt
    26581 ctgttctgcg atttgttgtg gtagaaagtc ttttgtaagg tcataggctt ttgcttcttc
    26641 gtattgttct ttcgtaattt ggccttcacg atacatactg aataaaacaa aatcttttct
    26701 ggcaaggccg gcagaaagat cttcttttag cgcgcctgtg ttcgtgtatg gactatacgt
    26761 gatcgggctt tggggtaatc cagcgatata agcagcttgc ggcagcgtca catcttttgc
    26821 atctacacca aagattcctt ttgcagcttc ctggactcca gcaatgtttt gtcccttatt
    26881 attacgacca aatggtgaga cattcaaata cgtagcaatg atctcatctt ttgaaaagta
    26941 tttttctact tgtgcagaaa gaatgatctc gtttgcttta cgtttgaatg ttgtttcatc
    27001 tgtaaggatc tgctgtttga ctaattgctg tgtcaaagtt gatccaccag aagagccaat
    27061 accagtagct tcagatacta atgctcgtaa aacagcttta ggaacgtaac cttggtgttt
    27121 atcgaagtat tcatcctctg tgctgataat tgcagttttc aataaaggtg aaatcttatc
    27181 agatgaaata gtcgtgcgca tcagatctgt ctggattttt gatatctctg tattatctgc
    27241 ataaacaatc tttgacgttt ctgtaatgtt tcctagttct gtctgcagtg cttttttagt
    27301 cggcaattgt gtgtcttcga cgagataggc aaaatagcca gcgcctattc caatacctag
    27361 agctgcgaac atcaaaagaa gaacagtaat aaaaacagtc aatgattgaa aaactctaaa
    27421 aataatctta ggaacaaacc atttttcttt ttttcttcta ttctgttttt ttcgagtatt
    27481 tttgcgtggc aaaagggtca cctcaactaa tggttttatt tcgctaatta tagcaaataa
    27541 tagaacaaaa aaacagacaa ggctgttttt ggcaggaatt tcttttttaa aagagttaaa
    27601 acgtgagaaa atgatcttta ttataaaaga gttcttaagc ttttctttag agaaaatttc
    27661 agttactatg gagaacaggg caaagaaaaa tcaatcattt tctttgtata tcatcttacc
    27721 aatatatagg aattgttagt aacaattgcc tttcaaggag gaatccatcc atgttattag
    27781 gctcacacgt cagcatgagc ggaaagaaaa tgctgttagg agcagcagaa gaagcagcca
    27841 gttatggatc ttcgactttt atgatctata caggcgcccc tcaaaacact agaagaaaag
    27901 ccatcgaaga gatgaatatt gatgccggaa aagcgtacat ggcagaaaaa ggtctaacaa
    27961 atatcgtcgt tcatgcccct tatatcatta atttggggaa tacgatcaaa ccagaaatgt
    28021 ttccttttgc cgtccaattt ctgagagaag agattgttcg tgcggaagct ttaggcgcta
    28081 ctcaaattac acttcaccca ggtgctcatg taggggcagg aacagaagcc ggaattgcta
    28141 gaattattga aggcttgaat gaagttttga caaaagaaca gacggcacaa atcgctttgg
    28201 agacaatggc aggtaaaggt acggaaatcg gtcgaacatt tgaggaatta gcgcaaatca
    28261 tcgatggagt aaaattgaat gaaaaattat ccgttacttt tgacacctgt catacgaatg
    28321 atgccggtta taatattaaa gaagactttt caggtgtaat ggaagagttt gataagatca
    28381 tcgggttaga tcggcttaaa gtactccata tcaatgattc taaaaatccg caaggttcgc
    28441 ataaagaccg gcacgccaat attggttttg gaacaatcgg attcgacacg ctgaataaga
    28501 tcgtccatga tccaatgttt gacaatattt cgaaaatctt ggaaactccc tatgtaggca
    28561 aagataaaaa gaaagcttac gcaccatatg gaaaagaaat cgccatgttc aaagcgcaaa
    28621 cattcgatcc agaagtgttt cctgagttat tagcagaaga agtgatactc taagattttc
    28681 tatctgacag cagcaaaaaa ggaccaggca cattgccttg tccttttttg tttaagaaac
    28741 aaaggagtac tttttgcagt ctttttcatc atgcatcttc tcccccctct ttttaaattt
    28801 agacaaacaa ggtatactgg tagaaaagta aaaaaacgaa aagtttcaca agcaagtggc
    28861 aaaacgtgat tggaaggacg aaaagatgct gaacacttta aaacccttga tcaaccaact
    28921 aaaaaagaaa catacatact ttattcttgg catagcccta ttatttacaa ttattcgttt
    28981 gtatttatat agtaaagcaa tctatggaac agacatgtcg gcaacctatg atgaccagct
    29041 gctgattcgc tatgctcaaa atattttgga cggtaaatgg ctaggggagt atacgactac
    29101 aacactttcc aaaggaatct cctacagctt atttttagta cttgcacata aactgcatct
    29161 ttcttatgca gttttgttcg gcatgttcag tatttttgcc agtatattgg ttgcactcgc
    29221 gtttaaaccg attgtgaaga acaagtatct tcttttaagt atctattttt attttttgtt
    29281 ttctccgatt agttttacac atgaatattc cactcgaaca tatcgtaatg ccctcgtgat
    29341 tccggctgtt gagatcatta tcgcttgttt attagctatt tattaccgca aatacggttc
    29401 tagcagaaaa ttgctgccat ggtttttagg gctttctgtc gcttttcctt tcttttggtt
    29461 cattcgcgaa gattctatct ggcttcttcc ttttactgta gtagctttgg gtatcagttt
    29521 cgtccagatc ttgctagggc aagacttttc tttcaaagaa gtaaagaagt taaaaaaacg
    29581 actctttctt ctctcgaaac gggaatggct gaaactttca ttgtttttac ttcctatagt
    29641 tggcttttcc atcgtctatc aatggatcga atataaaaat gagacaacat acggtattgc
    29701 aaccatcaat gaccgttcca gtggagaatt tggacgtttg atgaaacaac tgatccgaat
    29761 agatgatgaa acgaacttga ataaagaaaa cagtgagata tgggtctcac atgctgcact
    29821 aaataaagca ttagccgcat ctcctacatt ccatactgtt tctacccgta ttgacgaatt
    29881 atatacgact cacgcgtgga ctcaaggtgg aaaagtaaaa gaattacccg gagatatcat
    29941 cttttgggca ttgcgagatg tggtaagcga atctggttat taccaaaaca atgcaaaaga
    30001 aaccaatgat ttttggaaaa aagtcaacca agaattagaa gaagcctatc acaagggaac
    30061 aatcaaaaag aaaaaagagt tctatctgat gggatcagga gatggaaaga cgatgagtga
    30121 tctgccaatc gtattcgatt tccttcaatc aggatacgct tatgcatggc gatataaaga
    30181 tttccatcaa ggaggaaatt atagtttcgg aacgcaagaa caagtcaaac aggctcaaga
    30241 attattaaac cttccgctaa tggagaattg gatcgatagt aatgaaccga gaacacctaa
    30301 tttccaatta acaaaatcag caaaagtcgc gaatgtcatc ataaaaattt accaagttct
    30361 ggctattccc gtgctgattg cttcaatagc agggatatta cttatgatac taggaaccgt
    30421 atttgcaaaa gctgatcgtg cctattatag ttcctctttg attgtggtca ccggattaat
    30481 actgacacaa ttggtttttc ttttcggtgt ctcatggttt tgttcttatg cgccagaaaa
    30541 aaaggactat tttttaagtg tctacactgg tgcaggcgtg ccattaacgc aattcatcgt
    30601 agtaacaagc atgctagttt tcagtaaatt aaatctgaaa aaagctttat cttctaaata
    30661 aaaagaccct tggtagctag atttttaacc taactatcaa gggtctttgt gtggaaaaat
    30721 cagtttttat tcgatttctt ctaccgtttc ctgcaaaagc tgttcatatt ttttgtttcc
    30781 tttatataaa tcaccaatcg tccatgccaa caggagaagg acaagaacaa tgattacata
    30841 agcgatgtga tcagccaatg ctagttgagc ggaagtggct tgctcgccga agaaactttc
    30901 gatctgtcct ggtaatcctc gtaagttcaa gtaagttaga ctaatcacag agaaccagcc
    30961 aagaagtttt acgatgaatg aatttttaaa tcggctaccc atctccgctt ggctgttggt
    31021 catcatcagt aaaggaatca tcgaaaacgg caacgcaaaa gccaagaaca cctgggattc
    31081 attcattaag ttattcaaag cggcatgttc ttcgatcgtt ccttttgtac ttgtaaacaa
    31141 aacacaaatc aatacaggaa tcacagaaag caatcgtgtg accagtcttc ttagccaaat
    31201 cgggatacgc atatggatga atccttccat gatgacttga ccggtcaacg taccagtaat
    31261 agtagagttc tggccagaag caagaagtgc tacagcaaat aaagtggaca agataccaga
    31321 ttttgctaca gcaatcaaaa taccattgct catagctgat ggatcagata aagcttcata
    31381 cagaccaaaa aaggaaggat cttggacaga accggttttg aagacagcga cacccatgat
    31441 caatagcagt gcattgacta gaaaagccat tgacaactgg atattagaat cccaagtaga
    31501 aaaacgtaca gcttgtgcta catcttcttc gttttggtga tcgatcttcc gagtttgtga
    31561 aactgctgaa tgaagatata agttatgcgg catgactgtt gcaccaataa tccctaaagc
    31621 tccagtcagc ggagtttcgc cgccgatgac cggtgagtct gagaatgtat gactgttagg
    31681 gataaagcca gcaaataaac tgtgccaatc tgggttagac aagccaactt gatagacaaa
    31741 aacgaataaa atgacgaaaa ttaagcagac aaccagtgct tcgatcttac gaaacccgac
    31801 ttttgtcagt agtaagagaa cgaaaacatc taatacagta ataaagacgg caatcaccaa
    31861 tggaatatca aaaagtaagt agagggcgat tgctgcacca atgacttctg caatatctgt
    31921 tgccataatg gctaattcag tcaagatcca taaaatgaca cctaatgtct tacttgtgcg
    31981 tgctcggatt gcctgtgcca aatccatttg gctcacgatt cctaattttg ctgccatata
    32041 ctgtaaaagc atagcaatca gactagacat caaaataact gacatcaata aatattggaa
    32101 attttgtcct ccggtgatcg aagtagacca gttacccggg tccatatacc caactgctac
    32161 taacgcacca ggtccagagt aagcaagaag tgttttaaaa aaacttttcc cctttggtac
    32221 atcaactgtt ccatttactt cagctaaaga taaaccattt gtatgttcaa taagtcgatg
    32281 cttatgaaat tgtttattgg attgattatt caagaaaaaa ccacctttcc tatttatcta
    32341 cgattacatt ctacacctca aaaagaaaaa attaaagaaa aagattcggt acgcctaatt
    32401 ttttttcatg gcgcgtcttg ctagatgacg aaagaaattt tgttctcctc taaaaaaacc
    32461 actatttttt gcatgaaact aaagaatacg cctttctttt ttcaataaac tttagtaaaa
    32521 taagtcattg agagcaattc ggaaatagaa aggagagatt tatatgttat caacaggtat
    32581 tgtcgtactt attgctatta ttgctttgct aatcggcgca gtaggtggct ttttcctagc
    32641 gcgtaaatat atgaaagact atttagaaaa aaatcctccc gttaatgagg acatgttgcg
    32701 atcaatgatg atgtcaatgg ggcaaaaacc atctgagaag aaaattcgcc agatgatgca
    32761 gcaaatgaag aaccaaggga aaaaataggt tctgacccaa cgacttttta cgggctgatt
    32821 ttttcagtcc gttttttatt tcgtaatttg ttagactatt cagattattc agcaaaaaag
    32881 acaagcacag acaatcctag gctcttgata aagacaaagg aggagtaaaa tgtcgatttt
    32941 tagaaaatta ggctggtttt ttcgtcagga aaagaaacat tatttaattg gggttttctc
    33001 gctattcgca gtggccttgg tacagttagt tcctccaaaa gtgattggga tcatcattga
    33061 tgagatcgcc gataaaaatt tatcaataaa gatcattttc gggtggatct tcctattgat
    33121 tatcgctgct tttttacaat atctttttcg ttatatctgg cggacgaata tttggggaag
    33181 tgcagctcga ttggagaaag aacttcggca gcagttattc aaacatttta cagaaatgga
    33241 caatctcttt taccaaaaat atcggactgg ggatttgatg gctcacgcaa cgaatgattt
    33301 gaatgcgatc cagaatgtag caggagctgg tatcttgacc tttgcagatt cagtgattac
    33361 gggcggaaca acgatcattg cgatgatttt gtttattgat tggcgcttaa cactgattgc
    33421 gctgattcct ctgcctttgc tggccgtcac ttcacgagtg ctggggtcaa aactccatga
    33481 tgcttttcgt gattcgcaag aagctttttc tagtatcaat gataagacac aagaaagtat
    33541 cactgggatc aaagtcatta aaacatttgg acaagaaaaa gaagatctag cggattttga
    33601 agaaaagatt gaagatgcaa ttattaaaaa caaacgagtc aattttttag actccttatt
    33661 tgatccattc attaccttga ttatcggtct gtcttatgtt gcaacaatta ttttaggtgg
    33721 aaagtatgtc ttggaaggca cgatctcgat cgggcagatg gtctcattta ttagttatat
    33781 cggtatgctt gtttggccga tgtttgcgat tggtcgattg ttcaacgtat tggaaagagg
    33841 aaatgcttct tatgatcgcg tcaatgaact tttgcatgaa aagacgcata tcgtggaaca
    33901 actgggtgca gtgcagaccc cggcaaaagg cgagctgaat gtagctattt cctcttttaa
    33961 ttatccgaaa gaagaatcac cagcattaga acaaatcaag tttgcgatcc aagaaggaga
    34021 aacattaggt attgttggga aaactggtgc gggaaaaacg acgatcatca aattactgat
    34081 gcgtgaatac gatgattatc aagggaaaat ccaatttggc ggacgttcga tcaaagaata
    34141 ttcattgaat gctttattag gatcaatcgg ctatgtgcct caggatcact tcttgttttc
    34201 gatgaccatt cgagataata tccgtttttc taatccaaaa ctttcagaag aagaagtaga
    34261 gcatgcggct tctttagcat ttatcaacaa tgaaatcaag gaattaccaa aaggttatga
    34321 taccttagtt ggagaacgtg gagtctcttt atctggcgga cagaaacaaa ggatttcgat
    34381 tgctcgagca ttgatcaaaa atcctgaatt attaatatta gatgatgcac tctctgcagt
    34441 agatgccaaa acagaagaag ctatcttgca taatttaaaa gaaataagaa aagagaaaac
    34501 aactatcatt gcagctcacc gtttaagtag cgtgatgcat gcaaaagaaa tcattgttgt
    34561 cgatcaaggg aaaatcattg aacgaggaac ccatcatgag ctgcttgcac taggcggctg
    34621 gtatgaacaa atgtgggaga aacagcaatt agaagcgaag atagaaggag gcgaggcctg
    34681 atggatgaaa attatcaatc ggaatggaca aaatcgattc cacttaaaga acagaaaaag
    34741 atcatcaaac gactgatgaa gttcgccaaa ccttttagaa gaacgttcat cgttgctatc
    34801 ttatttgcgt ttgctttgtc tgtgatcaat gtcctattgc caaggatcat ccaaactttt
    34861 atggatgatc atctggctaa acagtcggct acgacacaag ttattttgtt ttttgctggt
    34921 atgtacttat ttggagtcat agtaaaaagt atcatttggt ttttccaatg gtatttgtat
    34981 tccatggcct cattgaagac ctatcagtat attagggtaa aattatttga aaagctgcat
    35041 acactaggta tgcgttattt tgatcagaca ccggcaggat caaccgtttc tcgagtgacg
    35101 aatgacacag aaacgctatt tgaattttgg tacgtctttt tgatggtatt gactgggatc
    35161 tttgcagtcg tttcttcatt ccttgccatg tttcaaatca atggagaaat tgccttgtgg
    35221 actttggtgt ttttgccgat tttaggaatc gtcatctggt attaccagaa attcagttct
    35281 cgtatttacc gaaatatgcg ggaaaaatta agtcagctga acacgaaatt gaatgaatcg
    35341 atttccggca tgcgtattat ccagcagttt cgccaagaag ctcgtttagc taaagaattt
    35401 gaacaagtga acaatgagta tttagacacg cgttatgcga tgatcaaaac gaattcgatt
    35461 ctgttaagtc cgatcatcaa ttttctatat gcattggcga ttgcgttggt gttgactttg
    35521 tttgggatcg atgcgttgca ttctcccgta gaagttggac tgatctatgc atttacgacc
    35581 tatgtccagg gattttttaa tccaatgtct caaatgatgg actttttgag catcttcaca
    35641 gatgggatgg ttgctggtag tcgtatcttg aagattatgg atacggaaga atatgcacca
    35701 caacaaaacc ctggagcaga tcaaatgatt acttcaggta agattgagtt tcgcaatgta
    35761 agcttttctt atgatggaga gcacaatgta ttaaacaata tctcttttgt tgctaatccg
    35821 ggagagaccg ttgcattagt gggacacacc ggaagcggaa aaagctcgat catcaacgta
    35881 ttgatgcggt tttacgaatt ttatgaaggt cagatattga ttgatgatca tgacattcga
    35941 gaatatccaa tggaagagtt acgtaagaaa atggggctgg tcttgcaaga tgctttcttg
    36001 ttttatgggg atatcgcagg aaatattcgg atgatgaatc cagctatcac agacgaacag
    36061 atacgccatg cggctgagtt tgttcaagca aacaaattca tcgatgaatt accaaaagga
    36121 tatcactcaa aagtgatcga acatggtgcc agctattcta gtggacaacg acagctgatc
    36181 acttttgcgc gaacaatcgt gactgatcct aaaattctag tattggatga agcaacagca
    36241 aatatcgata ctgaaacaga aggattgatc caagaaggat tggcaaaaat gcgtcaagga
    36301 cggacaacga ttgcgattgc ccatcggctg tcaacgattc gagatgcaga attgatttta
    36361 gttttagaca aaggtcatat agtagaacgc gggaatcatg aaacacttct agcgcaaaat
    36421 ggtttgtatg cagatatgta caaactgcaa agcagtggta tataaaagat gttgtgacag
    36481 tagaattttg acctaagcac tagagtgaaa ataaaacata gcttctcata tttttcatct
    36541 gtgagagtta gtgtcgtagt caccagattt aaaaaaagat tcgacagcta gtcagaagtg
    36601 cctaatagcc actaagaaag cgttgacaaa agcatttgct ttcgataata taaagaaaac
    36661 gatacaactt ggatagtgat tgcctgatgc aagctaaacc tgatgagtct cccatggttt
    36721 catggacggc tttttgggtt tttctttttg ttttggatac agccctatac atcatggaaa
    36781 ggagcaaaac atgctgattg agaaagtact gaataacaac gtcgtaataa ccagggatga
    36841 aagaaatcag gaaatgatcg tgatgggaaa aggtttggca ttcaaaaaga aagtgggtga
    36901 cccgattttt gactcatgca tcgacaaaaa atttcagttg acaaatgacg ggctttcaca
    36961 acagttccaa gaattggcac aagcgattcc tcttgagtac ttagaaattt cttgtgaaat
    37021 cattgaatat acaaaagagg ctctacaagt ccagttgaat gattctgtgt atattgcatt
    37081 gacggatcat ttgtatacgg ctatcgaacg tcacaaaatg ggaatatttg taccaaatgt
    37141 attgctgtat gatatcaaac aactatttcc taaagagtat gccatcggga aaaaaacagt
    37201 gaagaaaatc tcggataaat accacatcaa gttatcagaa gatgaagccg gatttatcgc
    37261 attgcattta gtcaatgcac aaggcattca aagccagcaa gacaatacct acctgtttac
    37321 tataacaata caggatattc taaatatcgt ccgctattac tataagatta catttgatga
    37381 agattcagtc catttttttc gattcgtcac acacttacgt ttttttctga cacgcgtcct
    37441 agatggagaa aaatcagaag tgggagaagt ttctgaggaa ttgtttcagg ttattcaaaa
    37501 aaaatataag gatacgtttg gttgcgttga gaaaatagca gactttttag ccgagacgtt
    37561 caactaccag atgtccacgg acgaaaaaat gtatttaacg atccatactg cgcgtttagt
    37621 gcatgagaat cgttgtaaat aaaaaaagga ggataaatta acaagaacgt atgaaataag
    37681 ggtagtgaca ttcagttggc taaaccttcc actaataaat tggagggaat agaaatggga
    37741 aaataccaag aattagcgag aaaaatcgta gaaaatgtcg gcgggaagga aaatatcaat
    37801 ggtttgacac attgtatcac aaggcttcgt ttcaaattaa aaaacgaaga aaaagcaaat
    37861 gatgagatct tgaaaaatat ggacggtatt gtaacggtaa tgcgagcggg agggcagtat
    37921 caagttgtca ttgggaatca tgtgccagtc gtgtttgaag aggttataaa agcaggaaac
    37981 cttacttttg atgaggctgt ttcaacagaa aaaatgagac cgtttgatat gcttattgat
    38041 ggaatatcgg gctgtttcca gccattcctt gctatcctag ctgctggcgg gatgattcgt
    38101 ggactgacag cattccttgt cttcttaggc gcgtttgacc gcggttctgg cacatttgtt
    38161 atgtttgata atatcggtga ttctgtattc caatttatgc ctgttattat cggtttaaca
    38221 gctgcccgta aatttaaagt aaatgaattc gtagggatgt tgcttggcgc agcattaatg
    38281 aatcctagct tgtcgctgga agctttgagt ggtgcagcag aagcaccact aacgaccata
    38341 ttttctggca ctatttttga agcacctatt tatcaaacag tattcggcat tccctggatt
    38401 gcgcgtaact acgcttcttc tgtcattccc attatcttta ttgtgttgtt agctagccaa
    38461 attcagaaac caattaaaaa gttagtacct gagatgatcg cgaatttctt cgttccattc
    38521 ttcaccgttc ttattacaat gcctttaggt tttttactcg ttggaccagt attcacattc
    38581 gcaactgata ttttaatggc tggatttgaa acacttttag cactttcgcc agttatttat
    38641 ggagcaatcg taggcttctt ctggcaaatt ctggtgatgt ttggtctcca ttgggcaatc
    38701 gtaccaatgg gattgatgca attttctgtg aatggttggc aaaatattat gacacctgtt
    38761 gcagtagtgt cttttggaca aactgcagca cttaccgcac tctactttaa gcttagaaat
    38821 ccaaaagaca aagcaattgc aataccagcc attgtttctg gtattgtggg gattacagag
    38881 ccagctattt atgggtttac attaccacgt aaaaaaatat ttatttttac ctgtgtcggt
    38941 ggtgcgttag ggggcgcgta tagtgggtta atgaatttaa cttcttggaa tcaaggtgga
    39001 ctaggcatct ttacaattcc caactatatt agaccagatg gtgatttaac agatgtgatt
    39061 aatgtattga ttgggatcgc cattgcgatg gtggtatcct ttacgctgac ttttttcttt
    39121 tggaaggatg aagcaggtga aacagatgat atacagaaaa aatcaggtaa agaaattgta
    39181 aaaacaccga tacaaggaca aatcgctcca ctaaatgcag caaaggatgc agcttttgca
    39241 caaggaacac taggtcgagg aatcttgatc taccctgaaa aaggagaagt acgtgcccct
    39301 tttgacggaa cagtcatgac cctgttcccg acgaaacagg caattggaat ggtttctgaa
    39361 acagggctgg aattgctgat tcatgtcgga ttggatactg tgcagttgga agggaaatat
    39421 tttgaaagct tagtgcagca aggataaaga gtgaaacaag gcgatctgtt gctacgcttc
    39481 gacaaaaaga aaatcgaaaa ggaaggatac agtttagaaa cacctgtgat cgtcaccaat
    39541 tacacggatt atttggatat tttggaaaac caaggggaga cggtcgggcc agacgatgca
    39601 cttatcaccg cactgacctg aacggttagg tgaagaatgg actaggctta actattcttt
    39661 ttattccact tctttatttt caaaattgaa aggctctttc atgatggctg agctgatagg
    39721 gcgtttatcg aatttttgac cgacagaaat tagatggact aaattctgtt gataattttc
    39781 tgcaaaaaca caactatata acacgtctag aagataacaa attgatgtat tgatcgtaaa
    39841 attaccaatt tttgaataca gtcgctcgcg tgtcgtaagt cgcaaagcgg ctgtactcat
    39901 tgcagatagc gttgaatcac cgatactggt caaagctaaa attggaacat gattttgttt
    39961 caatatttct gcggtacgaa ggatcacatt gttttcaccc gtataagaaa tcagaatccc
    40021 gcacatatca ctcgtgcaat tgtacgcttc atagaatgat tcaccaaaag tggtgacaat
    40081 cgtgacatcc attttgatgc gcttcatttt cagcatgaaa tcttgagaaa tcaaagtatt
    40141 cgagttactg gcgaaaatca cgattttttt tgcttgaagc attagttgtt tggctttttg
    40201 cagttggtca tgatgaatga gagaaagtgt atcatgaatc gtcatctgtt ctaaagaagc
    40261 cattttattt gcgatcgtca taataccgtc tgtggtggaa aaaggaagat tcgcatctac
    40321 gtcttgaaaa ttcccttcaa gataggctgt ttcagctaaa aaagcattac gaaaactgac
    40381 ccagccatca aaacccattt tttttgctac acggatgagt gtagaggggt ggacataaca
    40441 tcgctcagca atcgcttgga tggtcatttc gccaagtgct ggagaatcgc ttaagagaaa
    40501 ctggatcact tcattttctg aaggagaaaa ggagatttct ttgatttttt cacgtaataa
    40561 catgtagata ccttcttctt atttttttac gtaaattata acttttttga aaatgatttg
    40621 taaacggatt tatactatat aacttaaaat agacattttt tgacaagata tttacaaaaa
    40681 tccactagaa aacagtaaat tttgtaaatg ttactggaaa gaaacagatt tctattgttt
    40741 gttttgttta caaaaaagat aatgaaagtg taaaaaagta aaggagtgag aacatggcaa
    40801 aaggaatcaa aatcgctacg attggtggag gcagcagtta cacccccgag ttgatggaag
    40861 gatttatcaa aagatatgag gaattaccga tacgagaaat ctggctcgta gatgtagaag
    40921 aaggaaaaga aaagcttgag atcgttagtc agatgtctca gcgtatgtgg gatgcttctc
    40981 cgtatgaagt caagatctat gcaactctgg atcgggaaga agcattgaaa gatgcggatt
    41041 ttgttacgac tcagtttcgt gtcggtcaat tagatgcacg aatcaaagac gagcggatcc
    41101 ctagttatta tgggatgatt gggcaagaaa caaatggtgc tggcgggata ttcaaagctt
    41161 ttcgtaccgt tccagtcgtt ttggcaatcg ttgaggacat gaagcgttta tgtccaaatg
    41221 cttggctgat caatttcgcg aacccaagtg ggatggtgac agaagcagtt atcagatatg
    41281 gcaaatggga taaagtgatt ggattatgca atgtgccagt gatggcgatg atgacagaac
    41341 cagcaatgct tggcaagaaa aaagaagagc tgatctacaa gtttgccggt ctaaatcatt
    41401 tccattggca caaagtagca gataataaag gaaatgaccg cacggcagaa ttgattgatc
    41461 gtttatacga tgaagagaat ggattaccga aaaatatctt tgatatcccg tttttcaaag
    41521 aacaattaaa acaaatgaac atgatccctt gtggctacca ccgctattac tatcgtgaag
    41581 aagaaatgct ggaacatgct ttagaagaat atcaaacgat tggtacaaga gcgcagcaag
    41641 tgaaagaaac agaagcagag ctatttgaat tatacaaaga tccagaatta gaccataaac
    41701 ctgaacaatt gcaaaaacga ggtggcgctt attattcaga cgctgcctgt gaaacgattg
    41761 cttcaatcta tggaaataaa gaaacacaga tcgtggtatc tacaaagaat gatggagctg
    41821 ttcctgacct gccggcagat tgtgtagtag aagtcactgc ctatgttggc gcacaaggag
    41881 cgagaaatgt agcgtttggt tctcttcctg ctgctgaaag aggctggttg caagtaatga
    41941 agaatatgga attgctgacg atcgaagcag ctgtgactgg tgattatggg acagctttac
    42001 aggcgtttac gatcaatcca cttatacgat caggaaagac agcaaaacgg atcatggatg
    42061 agttgtttat tgcacaccag cagtatttac cgcaattcaa ggagacaatc agccgattaa
    42121 aagaagaagg aatcgaagtt caagatgaac tggcaagaga attatcaggt aatacacata
    42181 taatggagga agtataatgg gattcagaaa agatttttta tggggcggtg ctacagcagc
    42241 caatcaatgt gagggcggtt atgatgaagg tggacgcgga ctagcaaacg tagatgttgt
    42301 gccaatcgga aaagaccgtt ttcctgttat caccggaaag aaaaaaatgt ttgaatttga
    42361 tgaggatcat ttttatccag caaaaattgc aatcgatatg tatcatcgtt ataaagaaga
    42421 tattgctttg tttgcagaaa tggggttcaa gacttatcga atgtccatcg cctggagtcg
    42481 tatttttcca atgggggatg agaaagagcc gaatgaagct ggcttgaaat tttatgagga
    42541 tgcctttaaa gaatgccaaa agcacggaat tgagccactt gtgacgatta cgcattttga
    42601 ttgtccaatg catttgatca aagaatatgg tgcgtggcgt tccagagaac tcgttggttt
    42661 ttatgaaaat ctgtgtcgag tcatcttcaa tcgctataaa ggacttgtca aatattggct
    42721 gacattcaat gagatcaata tgatccttca tgcaccattc atgggcgcag ggctgtattt
    42781 tgaaaaagga gaaaatgaag aacaaatcaa atatcaagcg gcacatcatg agttggttgc
    42841 aagtgccatc gcaacgaaga ttgctcatga agtagatccg gagaacaaag taggctgtat
    42901 gctggcagca ggagcatatt atccatacac ttgcaatcct gaagatgttt gggcttctag
    42961 aaaatcagat cgagaaaatt atttcttcat tgatgtccag tcacgaggag aatatcccgc
    43021 ttatgcattg aaagaaatgg agcgaaatgg cattcagatc gaaatggaac caggggatgc
    43081 cgcattgctg aaagaacata ctgttgattt tatctctttt tcttattatg cttcccgagt
    43141 ttcgactacc gacaacgaac tcttggaaca gactgcagga aatatctttg cttctgtcaa
    43201 aaatccatat ctgcaagcaa gtgaatgggg gtggcaaatt gatccattag gcttgagaat
    43261 aacgatgaat gatatttatg atcgttatca aaaaccattg tttattgtag aaaacggctt
    43321 aggtgcagtc gatacgccag atgaaaatgg ttatgtggaa gatgattacc gaatcgatta
    43381 cctggcagcg catatccagg cgatgaagga tgcagtagaa ctagacggag tcgatctatt
    43441 aggctatacg acttggggat gtattgattt agtatcagct ggtaccggtg agatgaagaa
    43501 aaggtacgga ttcatttatg tcgatcgtga taaccaagga aatggcacat tagcccgttc
    43561 aaagaaaaaa tcattcaact ggtataaaaa agtaattgat tcaaatggag aaaatctttc
    43621 gaacgattga tcaatgattt ataaaagtct attgtttttc agcggatttt tcgagtataa
    43681 taaagctatt cgagaaacgc aagcagaaca atagactttt tttcgcattg gctgtaaagt
    43741 atgcttactt gattaagtaa aggagaatct acatatgtat gatgtaatcg ttattggcgc
    43801 aggtcctgct gggatgactg ctgctttata tgcttcacgt tcaaacttat ccgtattgat
    43861 gatcgaacaa ggtgcccctg gcggccaaat gaacaatacg gcagaaatcg aaaattatcc
    43921 tggattcgat tcgatcatgg gccccgatct agcttataag atgtatgaaa gtgtctcaag
    43981 atttgggaca gaaaatgctt atggaatcgt tcaagatatc aaggatcacg gcagccataa
    44041 agaagtcatc tgtgaagata aaagttacga aggaaaaaca gtcattatcg caacaggatg
    44101 tatccaccgt aaattaggcg tacctggcga ggaagaattt gctggacgag gcgtttctta
    44161 ctgtgcagtc tgtgatggtg cgtttttccg taacaaacgt ctagtagtca tcgggggtgg
    44221 ggactcagca gtagaagaag ccatctattt gacacaattt gcttctgaag tagtgatcgt
    44281 ccaccgtaga gatgaattac gtgcacaaaa gatcatccaa gaccgtgcat ttgcaaatga
    44341 aaaaatttcc tttatgtggg atacagtcgt tgacgaaatc gttggaaacg aaatggtagt
    44401 gacaggtgtg aaagcacgta atgtcaaaac cggcaatgaa tttgatttag cagcagacgg
    44461 agtattcatc tatgttggac tggatccatt aactgcacct tttagagaag caggtttgac
    44521 gaacgaagct ggctggattc caacagacaa cgaaatgaaa acgaatatgc caggcgtttt
    44581 tgccatcgga gatgttcgtg aaaaagattt gcgacaaatc actacagctg ttggtgacgg
    44641 cggtattgct ggacaacaag tgtataaata cttggagtct ttagaagaaa caacagcata
    44701 agtttaaaaa gagtagaaga acgggttcaa ttgttctttt actctttttt ttcgcgtgaa
    44761 atttactgaa tctgatttat gcgaacacgt ccaacacctt ttgataaaag aatcctttct
    44821 tgatttaagc gttttttggt cctcatactt tcatatctaa taagtgaatg ctatacttat
    44881 taagaaccat atttcagaaa gaggtgttat tttcatgtct tgggaacaag tgtatgaaca
    44941 atgggtacat gaagaaaatt tagaagaaaa cttaaaaaaa caattaaatg atctgggtga
    45001 agatcctgaa aaacttgaag atgcttttta tgcaccgttg gaattcggta ctgcaggtat
    45061 gcggggaatc ttaggacctg ggatcaatcg aatgaacatc tatacggtaa gacaagcaac
    45121 agaaggacta gcacgtttca tggatacaca aggtgaagaa acaaaacgcc gaggtgtcgc
    45181 tatcgcttat gactcacgcc atcagtcacc agaatttgca atggaagcag ccaaaacact
    45241 agcacatcac aatatcccgt cttatgtatt cgaaagtctg cgtccaacac cagagctttc
    45301 ttttgcagtt cgtcatttga agacgtttgc gggaatcatg gtaaccgcat ctcacaaccc
    45361 agcggcttac aatggatata aagtctacgg agaagatggc ggacaaatgc ctcctgcaga
    45421 cgcagatgca ttgacgaaat atgtccgtga agtcagcaat ccgctaaaag tcgaagtatt
    45481 atctgacgaa gaagctaaac atagcgggtt gatcacgatc atcggagaag aagtggacgc
    45541 tgcttatcta gaagaaatca aagcagtgac gattgatcgt gaattggttg aaacaatggg
    45601 gaaagatttg aaacttgtgt acacgccact tcatggaaca gggaaaatgt tgggcgaacg
    45661 tgcgttgaaa caagctggat tcgaacaatt cgtactagtt cccgaacaag ctgtggcaga
    45721 tccagatttc actacggtca aatcgccaaa tccagaagaa cattcagctt ttgaatatgc
    45781 tatacgactt ggagaaaaag aaggagctga cttgctgatt gcaacagatc ctgatgcaga
    45841 ccgtttaggc gcagcggttc gtttgccaga tggttcttat gaagtattga ctgggaacca
    45901 aatcggagca ttgtttgctc aatatatttt ggaagcacat caatcaagag gcactttacc
    45961 agaaaacgca gcggtattga aatcaatcgt ttccagcgaa ttgcctacag caattgcgga
    46021 acactacggt gctaccatgt tcaatgtgtt gacagggttt aaattcattg ctgaaaaaat
    46081 ccaacaattt gaagaagacc attcacatac ttttatgttt ggattcgaag aaagttatgg
    46141 ttatttagtt aaaccttttg ttcgcgataa agacgcgatc caagcattgc ttttattagc
    46201 agaagtagca gcttactata aaaaacaagg caaaacgttg tatgacggct tgcaaacgat
    46261 ttttgaaacc tatggctatt tcgcagaaaa aacgatttct gtgacaatga gcggacaaga
    46321 aggttctgcg aaaatctctg ctttgatgga aaaattccgt aaagaagcac cgacagaatt
    46381 tgccggagca aaagttattc aaacagaaga ctttaaagaa ttgacgagaa cagaccgtga
    46441 aggaaacgta gaaaaactaa caacaccgcc atccgacgta ttgaagtata cattggaaga
    46501 aaatggctgg atcgctatcc gcccttcagg aactgagccg aaaatcaagt tttatattgg
    46561 tgtaaaagga agctctacag aagacgcagc cgaaaaagta gcctcttatg aagcgattat
    46621 caaaaagatg acagaagaat aaaaaacttg agactgatgg aaaaagtggt aaactttaga
    46681 agtcagtctt ttttcaagtt ggaggtgaat ggattgccag acacgcaaaa atcaaaagta
    46741 gagaaagtca gtcaattatt caaaatgttg agtgatccca ctcgtcttaa aattctgcta
    46801 tatctgaaag acggggaaca aaatgtgaca gcaatcaccc aagcagtgga aatggagcag
    46861 tctgctgtat cgcatcaact acgtttatta agagaaaatc atgtcgtcaa gtctcatcgc
    46921 gagggaaaag cgattttgta tagtcttgat gattctcatg tactggatat tttgaaccag
    46981 acactcaaac atgtggaaca tcaataacga taggtgaaaa tagataaaca ctggagtgtg
    47041 acaacgaaga aatttcgttg tcacactcca gtgtttttta agaaaaagac aaaaatgatt
    47101 aatcgctttt ttctgttgtt tcagttccat aataactatg atcttctagg tctaaagcat
    47161 tcaaaataag cgaagccgtt tcttcaatag ataaagatgc cacattgatc actacacatc
    47221 ctagtttttc ataaagatca ttcgcaaaat cgagttcagc tctaattttt tcgatatcgg
    47281 aataagcagt atcagggttt aatccgtaag agcgcatccg ttctttacgg atattattta
    47341 aaatatctgg gttattcgtt agtcccacga tttttttagg atcgatttcc cacagctgtt
    47401 tgggaatatg tgcttgggga atcaaaggta aattagcaac ttttaaattt ttatttgcta
    47461 aaaacaagct caaaggcgtt ttggaagttc gggatacgcc tagcaaaacg acatctgctt
    47521 ctaaaaaacc acgaggatct ttcccatcat cgtattttac agcaaattcc attgctttga
    47581 tccgtttgaa atagttctca ttcaataaat ggagggcacc aggttgccga attggcttga
    47641 taccagttaa acgttcaatt tctgatatcg gtggcgtcat cacatcgaaa ctaaataatt
    47701 gatgttcttc acaaaaagaa cgtgttatct cgatcagttc ttgattgatt aatgtatgca
    47761 ggacaagtgc tttttcttct aatgcatctt gcaatgcctg ccttaatgtt ttttcatctg
    47821 tcacgaacgt tctgcggaat aggttgaact caacggtagg atactgagcc atagtagctt
    47881 gcgctagttt tgtcgcagtt tctccagcag agtcggagat gacaaaaaaa gtaacgattt
    47941 ctgtagaatc ggaagacatg gataaaacct cacttgcatt ttttttatgt ttattatacc
    48001 atactctaga atgcgtactt cgtaatcatt ttgatttgga ggaataagtc atgaaaaaac
    48061 agacattgat cgaacaatca ttagaaacat tgaaaaataa cggttttaaa tatactaaaa
    48121 aaagagaagc attgttaact tatttagtga agcgtaaccg ctacgtatct gctaaagaag
    48181 tttttgacta tatgaatcag gaattcaaag gggtaagtta tgatacgatt taccggaatc
    48241 tccatgattt tgcagagatc gatttactgg aagaaaccga tttaaatggt gaaatgaagt
    48301 ttcgttttca ttgctgtcag ggggaaatcg gccatcacca tcattttatc tgtacagtgt
    48361 gcggaacaac gaaagaaatc catatgtgtc caatgaatta tttcgaagaa cagctaggtg
    48421 gatgcacgat cgaagggcat agattcgaga ttttcggaag atgtgaaaat tgtcaataag
    48481 gatttaaata aaataacatt tgataattta aaaaaaaagc aagatttcct tgacggtcaa
    48541 aacagcatta gatataatat ttacaggaca gtcttgtttt ttgagatata tttcaataac
    48601 aattacggtt actttttaag ctcggaggga gggaattaac atgtcaaaaa cagtcgttcg
    48661 caaaaacgaa tcacttgacg atgctcttcg tcgcttcaaa cgttccgtgt caaaagcggg
    48721 tactttacaa gaatctcgta aacgtgaatt ctacgaaaaa ccaagtgtaa gacgcaagaa
    48781 aaaatctgaa gcagctagaa aacgcaaaaa attctagttc tgtgatgaat tggagttggt
    48841 agtgtgtcac tactaagtag attaaacgat gacatgaaag cagcgatgaa agcgaaagat
    48901 aaggaatctt tacaagtcat ccgaatgatc aagtcatcta tacaaaacga gcagattaaa
    48961 gaaggtcacg acttgacaga agaagaagaa ctaacggtgt tgtctcgcga aatgaaacag
    49021 cgacgtgatt ctttgcatga gtttgaagaa gcaggacgtg acgatttagc tgaaaaagta
    49081 aaaagtgaaa ttgtaatagt cgaaaaatat atgccggaac aactttccga cgaagaaatt
    49141 cgacaacttg ttcaagaagc aatcactcaa actggtgctt catcaatgaa agaatttggc
    49201 aaagttatgg gtgcaattat gcctaaagtc aaaggaaaag cagatggaaa ccaagtgaat
    49261 gcgattgtga aagaactatt acaatcttaa ttttatcatt taaactgcgg acagtgattg
    49321 tccgcagttt tttgtgttcg taaaaaaaga actaaaaaac agctagaaga aagaataaga
    49381 ttctttcttc tagctgtttt tttcctgttt aatatctctt atgttaaatt gtgtttgatt
    49441 tggttattct tctggtagtt catttgcttc ttgtttatac cctgataggc gataggcgta
    49501 ataaaagagt aagctgccag cgtaaccaag taacgttggg accatccagc caagtcctaa
    49561 tgcagcaaaa ggaacgtgat gatgataaaa tcctagtaag cttgctacga cagtttgctg
    49621 actcactaat gcaggcgaat ttgccaacat atccaatacg gctggaacgg cagaaaagaa
    49681 catcgttact tgatagacaa atggactctt ttggaagaat tttgctgtta aggaaagcaa
    49741 gattaacgca agtgacaatg gatacaaaag catcaatact ggtaaagacc attggatgat
    49801 attatccaat ccggcattcg caacgacaaa agagacaaaa gtcgttaacc gcagccatgt
    49861 catataactg actttaggga acagtttatg gaaatcttgt gcaaaagaaa caacgagtcc
    49921 catagcggta gtgaagacac ctaatgtgac cattacccct aagaagctag caccgaagtt
    49981 acctaaataa aaattagtga tctgagaaag tgcagttccg ccattttctg ataaaggcat
    50041 cttattcaaa ctcatcgcac cgagaaggac cagaccaaaa taaatcatga cttctaacgc
    50101 aacgctcagg ctaccagctt ttgctgttaa ttctgtgact tttttatctt taaaaccaag
    50161 atcacgaact gcatggacaa aagtgacact taaagcaagt aaagctactg catcaaccgt
    50221 gttgtatcct tctaaaacgc catttgtcaa agcgttcgtc tgataagaat ctgcagcaga
    50281 ttggtttaga ttccccatgg gttggaataa agaaacgaca aaaatgatag ctaataatgc
    50341 aagaaacaag gagtttaaat actttccgac atatttgacc aaacgtgttg gatgtacagt
    50401 aaggaaataa gcaagtaaaa agaaaagagc agagaataaa aacataccta atgaatcata
    50461 tttttcaggc aaaaagggtt tagcagccat ttcaaaagca gttgctgctg tccgcggtgt
    50521 accaaagaat ggtccgatcg ttaaatgaac aaggatcaag aacaaggcag cataccattt
    50581 tccgacaggt ttagctaagt catacagacc atcacttttt gtcactacta cagctaagat
    50641 agctaataat gggaaaagag cgcctgagat ggcaaatcct acaccagcgc tgatccagtt
    50701 tgatccagcc atttgaccta agtggacagg aaagatcaga ttacctgacc cgaagaacat
    50761 cccgaagata agtgaactga ccatcaataa ttgcttccat gaataagttt ttgtttcttc
    50821 cataaatttt tcctccaata attatcattt tgagtaacaa aataaacgtt gcagaaacta
    50881 gaaatgccac taatatcaga ttccatgaaa accaccacct taaaatataa aaaaaagccc
    50941 ttttccactt gggaaaaggg ctctagattc tagaacggta ccaccttttt tcgcttaaaa
    51001 aagcttcttc attcacggac gcttttcgat aaagttatcc gctagcctga taatggaggc
    51061 agccacaatg atctacgttt tcgacattga ctcggaaatg atgttcgtct attcgattta
    51121 ttgtcatttc tcacttacat gactcactgg gaataaatcg ctgaatagcc tactgttttc
    51181 ctcgacgctt tgctgtatat ttatttgttg acgttataat acacctgttt tttttgaaat
    51241 gcaagaatat ttcaaatatt ttgttttctt tttttgaaac aagaaaaata cccagagaat
    51301 acttgctaag ttagacggaa tacttgtcct ctatcttatg catagcttac atgatcagct
    51361 ttttttattc aaacgagacc tttagagaat ggaagcagct attttttaac gtttcttggt
    51421 acttatggtt aagcaactct tttagaacct tttcttattt gctatatagt ttcttctgag
    51481 tatggtaaaa taggaaagaa tgaatcgaaa aaaaggggat gtctattttt gacggaagag
    51541 tctagttctt tagagattag attgacggct gctgatgatg tcagcatgct tttaggtgca
    51601 cacgacaagc atataaaact gattgaagaa acaacagaaa caaccatcca tacacgtgga
    51661 gaaatcattc agatcattgg cgaacaatcc cagatttcct tggctgcctc agtcattcgg
    51721 acattgcagg aattgatcaa acgaggaatc catgtaagta caccagatgt cgtgacggca
    51781 ctgaaaatgg ggcaaaaagg cacattagat tattttgttg aaatgtatga ggaagagatc
    51841 gtcaaagacc gtaatggaaa accaatacgg gtaaaaaaca gcggccaaaa gaaatatgtc
    51901 gaatcggtag ccaaacatga tatcgtattt ggcgtaggac cagccgggac aggaaaaact
    51961 tttcttgcag tagtattagc aatcgctgcc ttgaaaaaag gtgaagtgca aaaaatcatt
    52021 ttgacgcggc ctgctgtgga agcaggggaa aatctaggct ttcttcccgg ggatctaaaa
    52081 gaaaaagtag atccttatct gcgtcctgtt tatgatgctt tataccaaat ttttggatta
    52141 gatcatacga accgattgat ggaacgaggc gtgatcgaaa ttgctccttt agcttacatg
    52201 cgggggcgga cattggatga tgcattcgtt attttggatg aagcacagaa cacgacagtt
    52261 gctcagatga aaatgttcct gacacgtctt ggttatcaat caaaaatgat tgtcaatgga
    52321 gatacgagtc agattgatct gcctcgaggg acaacaagtg gtcttgttca tgcagaacga
    52381 acattaaagc aaatcaagaa aatcgatttt gttaattttg aagcaagtga cgttgttcgg
    52441 caccctgtag ttgccgaaat catcaaagct tatgaaaagg cagatctcca tcaagaataa
    52501 gctttgctta ggaggataag cacatgttga ataaaacact gcagaaacta caagaaaaac
    52561 ttggaaaggc agcattgcct ttcctgctgt tcttgttttt tatcattatc tgcggcgtta
    52621 catttagtag tgtcaaacaa aaggccaatg atttcagaga aggccaagta gcagaagaaa
    52681 gtatccgtgc gaacaaaacg atcgagaaca cggaagaaac cgaacaaaaa agaaaactgg
    52741 cagcagaagc agtcactcca gaatacactt accaaaaaga tcttgcagat gaccagaaca
    52801 atcggatcaa gcaactattt gaattgatcg ataaaacgaa tgatgccatc aacaaatctt
    52861 atgacgaaaa gattgataag gcaaaggaag atgaaaatat cccaaagcct actacagaag
    52921 agcggatcgc cgcgttgaag aaaaattttg aaaatgtgaa tgcagaaaat gttgcgtttt
    52981 atcaaaaact accgaacaat ttttattcga cgatatttga aatgactgaa actgagatcc
    53041 atactgtgag ggatgagagt ctgaagctca ttgacgaaca gatgagcaaa caagttcgtg
    53101 aaagtgaatt ggaagctttt aaacaagaag cagaagagca aatccaatat ttgaatgtca
    53161 caccagcaca acaacaaatg attcgctact tagtggatca aggtatcgta gtgaatgacg
    53221 tattgaatga gaaaaagaca gaagaattga aacaatccgc acgagaagct gtacaaccag
    53281 tgatgatctt ccaaggagaa atcattgttc gtgaaggtaa tcaaatcgat gcttcagcta
    53341 tgaaaaaatt ggaattacta ggactgacta atcaaacaac ttcgattttt ccgctaattg
    53401 ccatggtttt agctgttttg cttcaaatcg gcgtactgat ttataattca cttcaattcc
    53461 aaaatgcagg agaaagaaca aaatacgtgc ttttctatgt gacagctatg tcagtcagca
    53521 tagtgctgat gaaatttttc caattattcc aaacggaaca agctacttat atcccgctgt
    53581 tttatcccgc tgcttttgca ccacttgttt taagcttttt cttgaaccga agagcaggga
    53641 tcatggctgg tattttccaa gcagtatctg cacttttcat tttttatgat tcgattggca
    53701 cgaactttct aacgattatc ttgatgtctt atgttttctc tgggctcatg gcaacgattg
    53761 tcagacgcaa acggatctca gaacaaggat tatctgctgc catgtgggtc attatttttc
    53821 ctgtgatgat ggatgtcatt ttgatcgtct atcaaggaat gagctttggt gatgcaacga
    53881 cttggttaat gcttgtctgc gggttcactg gtgcactctt ctcattcttg ctgacgatcg
    53941 gtctacaccc atatattgaa ttgatggtca atgatgacag tgtaattgta ttaaatgaac
    54001 tcagcaatcc aaatcatcct ttattgaaac agttgctgga agaagcgcca ggtacttatc
    54061 atcatagtat gatggtagct aatctaagtg ctaacgctgt agcagaaatc ggcggacgtt
    54121 ctttgctaac tcgagtagct tgctattatc atgatatcgg caagatcaaa catgctagct
    54181 ttttcgttga gaacttgcct tcaggagcgg aaaacccgca caatttctta ttgccagaag
    54241 acagtaaaca tattatcttt ggacatgtga cagatggagc caaaatattg gaagattata
    54301 atatgccgca aatggtgatc gatatttgca aacagcatca tggaaccacg ctaatgcgtt
    54361 atttctacat caaagcgaaa gaacgcaatc cggaagtaac agaagagcaa ttccgttatc
    54421 ctggaccaaa accgcaaagt cgtgaagcag ggatcgtcaa tattgctgac agttgtgagg
    54481 ctgcagtacg ggcaatggat catccgacga gcgaaaaaat cgaaagtttt gtccataatc
    54541 ttatcgaaga gcgtatttcc gatggtcaat tagacgatag tggactgacc ttgaaagaaa
    54601 tccgaaaagt ggaaaaatca ttgatcagcg gtttaagtag caccttccat tctcggatca
    54661 aatatcctaa aatgaaaaca gaagttgaga agataaagga agaacaagaa gaatagaaag
    54721 aagcgatgta taatggatat tacctttatg gatgaaacaa accaagtctc agaagagaaa
    54781 atgaaagaaa tcgatgactt gctgcaattt gcagcatctt atttaaagct gccagaggaa
    54841 acagaaatgt ctgttacttt catggacaat gcggcaatcc aagtaatcaa tcgtgattac
    54901 cgtggaaaag atgcaccgac agacgtcatc agttttgctc tggaagaaga aggcgaagac
    54961 gaaatcccag tgatttttga agatggagag aatccattgc ctcgtgtact aggagacatc
    55021 atgatttcta ttgaacgtgc tgctgaacaa gcagaagctt acggccattc atatgatcgg
    55081 gaacttggct ttttagcggt acatggattc ttgcatatca acgggtatga ccatatgaca
    55141 ccagaagatg agaaagaaat gtttggttta cagaaagaga tcttggatgc ctatggactc
    55201 aaaagataaa aacgtagaga aaaacaaaca tttcctgatg tctgtggaat ttgctgtcca
    55261 aggagtcaaa accgtttttg aggaagaacg caatatgagg aaacatgttg cgttcggtat
    55321 ccttgcttta tttatgggcg tgattttcca gcttgatcgg atagagtggt tatggctgct
    55381 tttagctgtt tttctcgttt ggatagtaga gatcatcaat actgtttttg aaaatgtagt
    55441 ggacatgttt acggattttc attttcatcc gattgggaaa aaaatcaaag atatggcagc
    55501 tggagcagtt ttagtgaccg cttgttttgc tgtcatcatt gggttgattt tatttgtccc
    55561 taaaatatat catttgttgt ttaattaaaa aggagaacgt atatgccaga acataaatca
    55621 gggtttgtcg caatcgtcgg ccgcccgaat gtaggaaaat ccacattatt gaaccgaatc
    55681 gtgggtcaaa aaattgccat tatgagtgac aaggcacaga caactagaaa taaaatccaa
    55741 ggagtatata ctacaccaga agctcagctg atttttatcg atacaccagg aatccataaa
    55801 ccaaagcatc gtctaggaga ttttatggta gaagcagctt acagtgcgtt gaaagaagtg
    55861 gatgcagtct tatttatgat cagtgctgat caaaaacgcg gccggggaga cgattttatt
    55921 attgaacgtc taaaaaacgt gcattctcca gtttatctga ttatcaataa aatcgataag
    55981 atccatccag acgagttgct gggaatcatc gaagattatt cgacgcagat gccttttgca
    56041 caagtgattc cgatctctgc tacagaagga aacaatgtcg aacgcttaat ggatgtatta
    56101 gtcagtgaaa tgccagaggg cccacaatat tttccagatg atcaagtgac tgatcatcct
    56161 gaatacttta tcgtttctga attgatccgt gagaaagtct tgtttttaac acgagacgaa
    56221 gtgcctcatt ctgtcgcagt cgttgttgat tcgatgaagc gaaacgagaa tgataaaatc
    56281 catatccagg ccacgatcat cgtcgaaaga gatagccaaa aaggaatcat tatcggcaaa
    56341 ggcggaaaaa tgctgaagca agtaggaacg aaagcaagaa aggatatcga aaatctttta
    56401 ggagacaaag tatttctaga actttgggtc aaagtccaaa aagactggcg agacaaaaaa
    56461 gtctatctgc aagactttgg ctaccgaaaa gacgaatact gatattctga acttcttata
    56521 ggttaagtat agaaataaac tggataaaaa tccgcacgtt gagaaaaacg gcggaagatc
    56581 gaaacataaa taatctggat cggcagaatc tgcttttcca gatttatttt ttaggaggga
    56641 gaatatgagc cagcttgcag aatcgaaagg cttgatttta tctagccgta attacaaaga
    56701 aaaagataaa ttagtcaaaa tctttacgga atcttctggc aaaatgatgt tttatatcaa
    56761 aggaatccat cggcaaaatc agccattagc tccggctttg ctccccttta ccgaagcagt
    56821 ctatatcggt aattttcgaa gtgaaggttt gtcgttttta agcagtgtca aagacgtcca
    56881 tgcgtttcgg aacatccaag aagatatctt tttgagcggt tatggtacct atttgctaaa
    56941 tctagtagat gcagcaatcg aagatcatca atatgacccg catttattcc aatttacgca
    57001 acaagctctg cagcggatgg atcaaggaga tgatgcagaa atcatcacga atatctttga
    57061 agttcaaata ctgcaacgct tcggaatcgc gccaatttgg acgcactgtg tcgtatgcgg
    57121 ggaaacaaaa ggaaaatttg actattcttc caaatctggc ggtgtgatat gcgagaaaca
    57181 ctggccaatg gacgaacacc gttatcatgc agatcctcgc gctgtttatt tcgttcgaat
    57241 gttttcggct atttcttatg ataagatcag tggaatccac ttgaaagaag aaacgaagca
    57301 ggcaatccgt gacctcattg accagttgta tgatgaatat gtcggtattc atctaaaaag
    57361 caaaaagttc attgatcaaa tgaagtcttg ggcacacgta ttaaagacgc cagagaaaaa
    57421 tatgcctgaa aaagagatac ctgaaaaaga agaataaatg aaagatacaa caaaaaaatc
    57481 aaaactattt gacaaataca gggacttccg tctattatta accgtagatt aaatagatcg
    57541 ttcactgaga ggatctatta tataaacaag aaaactgcaa aatgaatacg tacattgacc
    57601 aagatcagta aaaaacacgt ggtttagcga gttcgggaga gtggaagccg gacccaaacg
    57661 atttttgaag aggcacttgc gagtacgata aatgaagctg ccgaatcttt attcggaagt
    57721 agggtggaac cgcgaaatta ttcgtcccta tgtctgtaat cagacatagg gactttttgt
    57781 cgttttcttt cctgtattct tattgacagc aacgcttaaa aattgaaagg ggcaaaacag
    57841 atgagtaaaa aattgaccgt ccaagatatg attttaacat tacaaaaatt ttggtcatca
    57901 aacggctgca tgctgatgca agcttatgac acagaaaaag gagcaggtac gatgagccca
    57961 tatacattct tacgggcaat cggaccggaa ccatggaatg ctgcttatgt agagccatcc
    58021 cgccgaccag ctgacgggcg ttatggagaa aacccaaacc gtttgtatca gcatcatcaa
    58081 tttcaagtcg taatgaaacc ttcacctgaa aatatccaag agctttattt agaaagcttg
    58141 cgtcttttag gaattgatcc attagaacac gatattcgtt ttgttgaaga caactgggag
    58201 aacccatcga tggggtgtgc cggtgtgggt tgggaagtat ggcttgatgg gatggagatc
    58261 acacaattca cgtacttcca gcaagtcgga ggactacctt gtaaaccagt tacttctgag
    58321 atcacttatg gactagaacg tcttgcttca tatattcaag aagttgaaag tgtctatgat
    58381 ctggaatggg ctgatggtgt gaaatatggc gagatcttca aacaaccaga atatgaacat
    58441 tccaaatatt cttttgaagt aagtgaccaa gacttgctac taagcaactt tgaccgtttt
    58501 gaaaaagaag ctaaacgctg tatcgatgaa aatcttgttc atccagcgta tgattatatt
    58561 ttgaaatgca gccatacatt caatttatta gatgctcgcg gtgcagtatc cgtaacagaa
    58621 cgagcaggtt atctttcccg tatccgcaac atggctcgtg ctgtagccaa aatctttgtg
    58681 gcagaacgcg aaaaattagg attcccttta ttgaataaaa acacagcaaa tccagaaaac
    58741 atgaaggagg ctgaataaga tggcaaaaga cttattactt gaaatcgggc tggaagaaat
    58801 gcctgctcat gttgtcacac caagcatgaa acaattagaa gaaaaaacag caaaattttt
    58861 agatgaaaac caattgactt atgatacgat cgaaacgttt tctacaccgc gccgcttagc
    58921 aatcaaagta acaaacattc cagaacgtca agcagacagt gaagaagaag tcaaaggacc
    58981 agccaaaaaa attgctttgg acgcagatgg aaactggacg aaagcagctg aaggatttgt
    59041 ccgcggacaa ggtctgacga cagatgctat cacttttaga gaattaaaag gcgtggaata
    59101 tgtctatgta acgaaataca ctcacggcaa agaagcgaaa gaagtattga caggtttagc
    59161 agatgtcatt aaaagcctca ctttcccagt aacgatgcat tgggcagaat acgattttga
    59221 atatatccgc cctattcact ggatcgtggc attgttagat gacgaagtaa tcccgtttga
    59281 gatcttagat gtcaaaacag gcagagcctc tcgaggacat cgtttcttag gcgaagatgt
    59341 gacatttgcg catgcaaaag aatatgaaga aaaattgaat gaacaattcg ttattgcttc
    59401 accgcaaaaa agacaagaaa tgatcgttac acaagcaaat gaattagccg aaaaaaatca
    59461 ctgggttatt gatctagacg aagatttact agaagaagtc aacaatttag tcgaatatcc
    59521 aactgtcttt tctggcgact tcgaagaaaa atatctttct gttcctgaag aagtactagt
    59581 cacttctatg aaagaacatc aacgttattt cgaggtacgg aacgatcaag gtatgctgct
    59641 tcctcatttt atttctgctc gtaatggtga tcgggtaaaa ttggaaaatg tggttcgagg
    59701 caatcaaaaa gtattgacgg ctcgtttaga agatgcggaa ttcttttata atgaagacaa
    59761 gaaagcttca atcgaccaat acgttgaaaa actaaaaact gttactttcc atgaaaaaat
    59821 cggtagtatc tatgagaaaa tgcagcgggt acaagtcatt gcccgtatca ttggcgaaca
    59881 agtaggacta tctgaggaag aattgatgga tctgcaacga gctgctgaaa tctataaatt
    59941 tgatctagta accaacatgg taggggaatt tcctgaacta caagggatca tgggcgaaaa
    60001 atatgctctg ctgaaaggcg agaaacctgc tgtagcaaca gcgattcgtg agcattacat
    60061 gcctatatcc agtgaaggag agctacctca gtctacagta ggttctgttt tagcgattgc
    60121 agataaatta gatagcattt tttctttctt tgcagttggg atgattccaa gcgggtcaaa
    60181 cgatccttat gccttacgtc gccaagcata cggtgtcgtt cggatcatcg aatcgaaaaa
    60241 atgggccttt cctttgtctg ttctgcaaga aacgattagt gatgttatca gtaaagatac
    60301 ggatcgtttc ggcattggtt tatcagaagg gcaacaacag gtgatcgact ttatcaaagg
    60361 ccgtctgcgc caattattga caacgaagaa tattcgtcat gatgtcatcg aagctgtttt
    60421 gaatgcagaa caaaaagatc tgacaaaagt atttgcagca gcacaattgt tcaagcagca
    60481 tcttgcagat gaagacttca aaccatcgat ggaagctttg acacgtgtcg tgaacttagc
    60541 gaaaaaagcg gagcttgaaa aacaatcaga agtagaccct gaactgttcg aaaatgaagc
    60601 ggagaaagaa ctgcacaaag cagtagaggc aatgaaaaaa agctttgctg accaaacgat
    60661 caacgaaaat tatgatcagc ttgtaagcct tcgcccactg atcgaaaatt attttgatca
    60721 gacgatggtc atggcagaag aaccagcagt acgtaagaac cgattaacgc aattaagtca
    60781 aattgctgcc atggctttgt cgctagcaag tttagatcaa atcatcacaa aataatttct
    60841 aatatgtaaa taaaccagag aataggcaca aaa
//
LOCUS       DAFCNJ010000011        60191 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000011 DAFCNJ010000000
VERSION     DAFCNJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.011"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="LNE35_000862"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="LNE35_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBK6910040.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(524..724)
                     /gene="rpmI"
                     /locus_tag="LNE35_000863"
     CDS             complement(524..724)
                     /gene="rpmI"
                     /locus_tag="LNE35_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBK6910041.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(796..1320)
                     /gene="infC"
                     /locus_tag="LNE35_000864"
     CDS             complement(796..1320)
                     /gene="infC"
                     /locus_tag="LNE35_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBK6910042.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1364..1489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1640..2272
                     /locus_tag="LNE35_000865"
     CDS             1640..2272
                     /locus_tag="LNE35_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK6910043.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2274..3179)
                     /locus_tag="LNE35_000866"
     CDS             complement(2274..3179)
                     /locus_tag="LNE35_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK6910044.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3264..3923)
                     /locus_tag="LNE35_000867"
     CDS             complement(3264..3923)
                     /locus_tag="LNE35_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK6910045.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4239..5183
                     /gene="mvk"
                     /locus_tag="LNE35_000868"
     CDS             4239..5183
                     /gene="mvk"
                     /locus_tag="LNE35_000868"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBK6910046.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5176..6153
                     /gene="mvaD"
                     /locus_tag="LNE35_000869"
     CDS             5176..6153
                     /gene="mvaD"
                     /locus_tag="LNE35_000869"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBK6910047.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6166..7251
                     /locus_tag="LNE35_000870"
     CDS             6166..7251
                     /locus_tag="LNE35_000870"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBK6910048.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7248..8312
                     /locus_tag="LNE35_000871"
     CDS             7248..8312
                     /locus_tag="LNE35_000871"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBK6910049.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8561..9364)
                     /locus_tag="LNE35_000872"
     CDS             complement(8561..9364)
                     /locus_tag="LNE35_000872"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBK6910050.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="LNE35_000873"
     CDS             complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="LNE35_000873"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBK6910051.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10242..11249)
                     /locus_tag="LNE35_000874"
     CDS             complement(10242..11249)
                     /locus_tag="LNE35_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBK6910052.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11256..11900)
                     /locus_tag="LNE35_000875"
     CDS             complement(11256..11900)
                     /locus_tag="LNE35_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBK6910053.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12000..12758)
                     /locus_tag="LNE35_000876"
     CDS             complement(12000..12758)
                     /locus_tag="LNE35_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBK6910054.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="LNE35_000877"
     CDS             complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="LNE35_000877"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBK6910055.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="LNE35_000878"
     CDS             complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="LNE35_000878"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBK6910056.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="LNE35_000879"
     CDS             complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="LNE35_000879"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBK6910057.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="LNE35_000880"
     CDS             complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="LNE35_000880"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBK6910058.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="LNE35_000881"
     CDS             complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="LNE35_000881"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBK6910059.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18156..19025
                     /locus_tag="LNE35_000882"
     CDS             18156..19025
                     /locus_tag="LNE35_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBK6910060.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19151..20050)
                     /locus_tag="LNE35_000883"
     CDS             complement(19151..20050)
                     /locus_tag="LNE35_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6910061.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20215..21039)
                     /locus_tag="LNE35_000884"
     CDS             complement(20215..21039)
                     /locus_tag="LNE35_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK6910062.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21050..22345)
                     /locus_tag="LNE35_000885"
     CDS             complement(21050..22345)
                     /locus_tag="LNE35_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBK6910063.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22361..22840)
                     /locus_tag="LNE35_000886"
     CDS             complement(22361..22840)
                     /locus_tag="LNE35_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBK6910064.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22846..23874)
                     /locus_tag="LNE35_000887"
     CDS             complement(22846..23874)
                     /locus_tag="LNE35_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBK6910065.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23891..24997)
                     /locus_tag="LNE35_000888"
     CDS             complement(23891..24997)
                     /locus_tag="LNE35_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBK6910066.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="LNE35_000889"
                     /gene_synonym="adhC"
     CDS             complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="LNE35_000889"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBK6910067.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="LNE35_000890"
     CDS             complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="LNE35_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBK6910068.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="LNE35_000891"
     CDS             complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="LNE35_000891"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBK6910069.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29968..30666
                     /locus_tag="LNE35_000892"
     CDS             29968..30666
                     /locus_tag="LNE35_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK6910070.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30834..33473)
                     /locus_tag="LNE35_000893"
     CDS             complement(30834..33473)
                     /locus_tag="LNE35_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6910071.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34092..35123)
                     /gene="queA"
                     /locus_tag="LNE35_000894"
     CDS             complement(34092..35123)
                     /gene="queA"
                     /locus_tag="LNE35_000894"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBK6910072.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35316..36491
                     /locus_tag="LNE35_000895"
     CDS             35316..36491
                     /locus_tag="LNE35_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6910073.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36495..37136
                     /locus_tag="LNE35_000896"
     CDS             36495..37136
                     /locus_tag="LNE35_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910074.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37133..38050
                     /locus_tag="LNE35_000897"
     CDS             37133..38050
                     /locus_tag="LNE35_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6910075.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38047..38718
                     /locus_tag="LNE35_000898"
     CDS             38047..38718
                     /locus_tag="LNE35_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910076.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39259..39747)
                     /locus_tag="LNE35_000899"
     CDS             complement(39259..39747)
                     /locus_tag="LNE35_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBK6910077.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39948..40214)
                     /locus_tag="LNE35_000900"
     CDS             complement(39948..40214)
                     /locus_tag="LNE35_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK6910078.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40214..40477)
                     /locus_tag="LNE35_000901"
     CDS             complement(40214..40477)
                     /locus_tag="LNE35_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6910079.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40953..41138
                     /locus_tag="LNE35_000902"
     CDS             40953..41138
                     /locus_tag="LNE35_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6910080.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41280..43103)
                     /locus_tag="LNE35_000903"
     CDS             complement(41280..43103)
                     /locus_tag="LNE35_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBK6910081.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43701..46406)
                     /locus_tag="LNE35_000904"
     CDS             complement(43701..46406)
                     /locus_tag="LNE35_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBK6910082.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46712..47659)
                     /locus_tag="LNE35_000905"
     CDS             complement(46712..47659)
                     /locus_tag="LNE35_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBK6910083.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47849..48118
                     /gene="rpsN"
                     /locus_tag="LNE35_000906"
     CDS             47849..48118
                     /gene="rpsN"
                     /locus_tag="LNE35_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK6910084.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48294..48656)
                     /locus_tag="LNE35_000907"
     CDS             complement(48294..48656)
                     /locus_tag="LNE35_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK6910085.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48667..49788)
                     /locus_tag="LNE35_000908"
     CDS             complement(48667..49788)
                     /locus_tag="LNE35_000908"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBK6910086.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49804..50154)
                     /locus_tag="LNE35_000909"
     CDS             complement(49804..50154)
                     /locus_tag="LNE35_000909"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBK6910087.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50284..51795)
                     /locus_tag="LNE35_000910"
     CDS             complement(50284..51795)
                     /locus_tag="LNE35_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6910088.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52117..53469)
                     /locus_tag="LNE35_000911"
     CDS             complement(52117..53469)
                     /locus_tag="LNE35_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBK6910089.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53622..54698)
                     /locus_tag="LNE35_000912"
     CDS             complement(53622..54698)
                     /locus_tag="LNE35_000912"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBK6910090.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54894..56639
                     /locus_tag="LNE35_000913"
     CDS             54894..56639
                     /locus_tag="LNE35_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBK6910091.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56919..57356)
                     /locus_tag="LNE35_000914"
     CDS             complement(56919..57356)
                     /locus_tag="LNE35_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBK6910092.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57340..58713)
                     /locus_tag="LNE35_000915"
     CDS             complement(57340..58713)
                     /locus_tag="LNE35_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910093.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59035..59523)
                     /locus_tag="LNE35_000916"
     CDS             complement(59035..59523)
                     /locus_tag="LNE35_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6910094.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59545..60147)
                     /locus_tag="LNE35_000917"
     CDS             complement(59545..60147)
                     /locus_tag="LNE35_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK6910095.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17103 a        13016 c        10989 g        19083 t
ORIGIN      
        1 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
       61 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      121 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      181 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      241 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      301 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      361 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
      421 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
      481 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
      541 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
      601 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
      661 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      721 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      781 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      841 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      901 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      961 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1021 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1081 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1141 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1201 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1261 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1321 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1381 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     1441 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
     1501 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     1561 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
     1621 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     1681 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     1741 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     1801 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     1861 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     1921 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     1981 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2041 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2101 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2161 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
     2221 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2281 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2341 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     2401 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     2461 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     2521 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
     2581 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
     2641 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     2701 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     2761 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     2821 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
     2881 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     2941 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3001 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3061 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3121 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3181 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3241 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
     3301 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3361 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
     3421 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
     3481 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
     3541 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     3601 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     3661 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     3721 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     3781 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     3841 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     3901 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     3961 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
     4021 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4081 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4141 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4201 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4261 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4321 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4381 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     4441 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     4501 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     4561 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     4621 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     4681 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
     4741 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     4801 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     4861 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
     4921 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
     4981 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5041 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5101 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5161 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5221 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5281 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5341 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
     5401 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     5461 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
     5521 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
     5581 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
     5641 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     5701 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     5761 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     5821 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     5881 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     5941 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
     6001 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6061 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
     6121 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6181 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
     6241 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
     6301 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6361 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
     6421 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     6481 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     6541 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
     6601 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     6661 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     6721 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     6781 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     6841 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
     6901 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     6961 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7021 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7081 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
     7141 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
     7201 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7261 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7321 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7381 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     7441 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     7501 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
     7561 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     7621 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     7681 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     7741 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     7801 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     7861 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     7921 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     7981 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8041 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
     8101 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8161 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
     8221 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8281 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     8341 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     8401 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
     8461 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
     8521 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
     8581 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     8641 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     8701 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     8761 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     8821 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     8881 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     8941 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
     9001 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9061 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9121 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9181 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9241 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9301 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9361 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
     9421 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
     9481 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
     9541 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
     9601 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
     9661 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
     9721 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
     9781 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
     9841 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
     9901 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
     9961 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    10021 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    10081 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    10141 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    10201 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    10261 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    10321 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    10381 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    10441 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    10501 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    10561 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    10621 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    10681 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    10741 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    10801 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    10861 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    10921 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    10981 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    11041 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    11101 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    11161 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    11221 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    11281 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    11341 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    11401 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    11461 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    11521 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    11581 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    11641 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    11701 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    11761 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    11821 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    11881 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    11941 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    12001 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    12061 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    12121 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    12181 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    12241 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    12301 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    12361 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    12421 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    12481 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    12541 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    12601 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    12661 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    12721 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    12781 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    12841 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    12901 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    12961 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    13021 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    13081 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    13141 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    13201 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    13261 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    13321 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    13381 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    13441 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    13501 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    13561 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    13621 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    13681 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    13741 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    13801 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    13861 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    13921 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    13981 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    14041 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    14101 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    14161 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    14221 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    14281 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    14341 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    14401 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    14461 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    14521 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    14581 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    14641 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    14701 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    14761 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    14821 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    14881 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    14941 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    15001 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    15061 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    15121 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    15181 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    15241 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    15301 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    15361 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    15421 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    15481 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    15541 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    15601 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    15661 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    15721 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    15781 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    15841 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    15901 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    15961 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    16021 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    16081 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    16141 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    16201 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    16261 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    16321 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    16381 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    16441 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    16501 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    16561 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    16621 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    16681 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    16741 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    16801 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    16861 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    16921 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    16981 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    17041 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    17101 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    17161 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    17221 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    17281 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    17341 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    17401 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    17461 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    17521 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    17581 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    17641 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    17701 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    17761 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    17821 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    17881 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    17941 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    18001 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    18061 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    18121 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    18181 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    18241 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    18301 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    18361 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    18421 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    18481 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    18541 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    18601 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    18661 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    18721 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    18781 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    18841 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    18901 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    18961 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    19021 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    19081 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    19141 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    19201 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    19261 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    19321 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    19381 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    19441 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    19501 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    19561 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    19621 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    19681 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    19741 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    19801 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    19861 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    19921 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    19981 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    20041 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    20101 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    20161 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    20221 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    20281 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    20341 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    20401 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    20461 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    20521 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    20581 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    20641 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    20701 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    20761 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    20821 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    20881 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    20941 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    21001 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    21061 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    21121 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    21181 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    21241 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    21301 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    21361 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    21421 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    21481 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    21541 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    21601 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    21661 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    21721 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    21781 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    21841 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    21901 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    21961 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    22021 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    22081 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    22141 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    22201 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    22261 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    22321 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    22381 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    22441 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    22501 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    22561 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    22621 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    22681 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    22741 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    22801 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    22861 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    22921 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    22981 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    23041 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    23101 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    23161 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    23221 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    23281 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    23341 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    23401 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    23461 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    23521 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    23581 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    23641 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    23701 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    23761 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    23821 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    23881 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    23941 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    24001 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    24061 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    24121 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    24181 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    24241 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    24301 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    24361 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    24421 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    24481 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    24541 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    24601 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    24661 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    24721 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    24781 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    24841 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    24901 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    24961 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    25021 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    25081 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    25141 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    25201 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    25261 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    25321 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    25381 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    25441 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    25501 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    25561 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    25621 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    25681 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    25741 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    25801 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    25861 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    25921 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    25981 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    26041 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    26101 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    26161 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    26221 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    26281 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    26341 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    26401 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    26461 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    26521 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    26581 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    26641 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    26701 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    26761 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    26821 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    26881 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    26941 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    27001 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    27061 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    27121 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    27181 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    27241 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    27301 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    27361 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    27421 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    27481 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    27541 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    27601 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    27661 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    27721 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    27781 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    27841 gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta
    27901 aacacttttt tgtgattttt ttcacagtta tttttaaata taaaattgct ttatttacag
    27961 gtgtttgtat agaaaaatat ataaagttat ttattattat ttatatgaca cacccgatta
    28021 ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat aaaaaatgag
    28081 gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta tttttcttcc
    28141 ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac tgtctcagct
    28201 ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt ccctggtttg
    28261 accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc taaaacaact
    28321 gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat cgttacgact
    28381 gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg tttcttttgc
    28441 gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag cattggtaat
    28501 gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta gcagaaaaat
    28561 gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa gctttttgcg
    28621 ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag caaattatca
    28681 tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa attgtggtaa
    28741 gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat ataggcacgt
    28801 gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa atcatgggca
    28861 tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc atttcgagcg
    28921 attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc atcgatcaat
    28981 gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat caacggtgta
    29041 gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa cccgccgatt
    29101 gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg aagatcttca
    29161 aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc atgagctttc
    29221 aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta gtcgtaactt
    29281 tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa tttattttgg
    29341 atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg atttttgaaa
    29401 tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg aaagccgcct
    29461 gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc agcttcttct
    29521 actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat aacgccagca
    29581 cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt tcctactggc
    29641 atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa acgcgcgcca
    29701 gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat tcatttccct
    29761 actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa gtgctttttg
    29821 caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc tttttttatg
    29881 aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg gtatattggt
    29941 gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa acgccaagca
    30001 aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat ccctacattg
    30061 attggtattg ctactattct tcttattatg attcctatta tcttgtttgc caccaccata
    30121 tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag ttcaggaggc
    30181 ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat ttcttggact
    30241 tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga tgcatttcgt
    30301 ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat ctcgatcttt
    30361 acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta ttcttattct
    30421 caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc taaagtactg
    30481 gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact tttctggtta
    30541 gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat tggttatcta
    30601 tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat
    30661 atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg tttatcgaga
    30721 ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac
    30781 acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt taatcattcc
    30841 tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc gattggtacg
    30901 atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag gccgaatagt
    30961 tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag taacgccatt
    31021 tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct gctgcgtaca
    31081 ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt ttgaccgaca
    31141 tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa ataataacca
    31201 aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa taaacctcga
    31261 gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg ttccgctggc
    31321 tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa cagtagttgc
    31381 acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc
    31441 gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata aaccgaacgt
    31501 ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg
    31561 gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt acttgctttc
    31621 aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc cgtacgttgc
    31681 caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac agagagagct
    31741 ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc tgaaagtgct
    31801 tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt caccacatga
    31861 tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat tgctccttta
    31921 ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa gtctttttcc
    31981 agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc aacagcaatc
    32041 actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct
    32101 gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat gtatttttcg
    32161 cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag ctcgtttact
    32221 cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac agcccttacg
    32281 atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg ctcgaccgtc
    32341 acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt cattgaatct
    32401 tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat ttcattttgt
    32461 gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt ttcaactgct
    32521 ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta tgcaagtgtc
    32581 aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga aatcgaagtc
    32641 atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac taaagccgca
    32701 gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac ctgtaaaggt
    32761 gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc tgtagccatg
    32821 ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc tttaaatacc
    32881 atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc atctttttca
    32941 actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga tgcttcgatc
    33001 aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc acccactacc
    33061 agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc cgtttgttta
    33121 ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac aatcgccaat
    33181 accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc cccatgttct
    33241 gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa
    33301 tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc atcgaattta
    33361 ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc agtatctgga
    33421 tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc catcctattc
    33481 ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc ctttttttat
    33541 ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt
    33601 atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga ggataccatc
    33661 aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca
    33721 ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc gtatggatgt
    33781 ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg aataacgtaa
    33841 acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca
    33901 aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga tggaaagtta
    33961 caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct tatcgagagc
    34021 tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc
    34081 ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct
    34141 tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac
    34201 atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat
    34261 tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta
    34321 aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc
    34381 ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat
    34441 tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac
    34501 cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat
    34561 tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag
    34621 acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt
    34681 tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt
    34741 ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt
    34801 cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt
    34861 gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa
    34921 cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg taactcttca
    34981 atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa
    35041 cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa
    35101 tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct
    35161 atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc
    35221 taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat
    35281 agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa
    35341 aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg
    35401 agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca
    35461 accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa
    35521 ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc
    35581 ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag
    35641 aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt
    35701 tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg
    35761 cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa
    35821 tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga
    35881 tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca
    35941 tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa
    36001 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
    36061 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
    36121 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
    36181 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
    36241 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
    36301 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
    36361 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
    36421 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
    36481 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
    36541 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
    36601 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
    36661 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
    36721 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
    36781 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
    36841 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
    36901 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
    36961 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
    37021 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
    37081 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
    37141 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
    37201 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
    37261 agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac
    37321 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
    37381 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
    37441 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
    37501 cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa
    37561 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
    37621 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
    37681 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
    37741 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
    37801 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
    37861 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
    37921 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
    37981 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    38041 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    38101 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    38161 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    38221 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    38281 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    38341 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    38401 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    38461 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    38521 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    38581 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    38641 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    38701 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    38761 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    38821 cttatgggta aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    38881 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    38941 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    39001 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    39061 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    39121 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    39181 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    39241 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    39301 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    39361 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    39421 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    39481 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    39541 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    39601 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    39661 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    39721 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    39781 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    39841 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    39901 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    39961 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    40021 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    40081 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    40141 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    40201 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    40261 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    40321 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    40381 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    40441 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    40501 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    40561 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    40621 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    40681 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    40741 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    40801 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    40861 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    40921 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    40981 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    41041 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    41101 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    41161 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    41221 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    41281 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    41341 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    41401 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    41461 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    41521 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    41581 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    41641 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    41701 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    41761 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    41821 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    41881 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    41941 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    42001 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    42061 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    42121 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    42181 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    42241 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    42301 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    42361 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    42421 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    42481 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    42541 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    42601 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    42661 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    42721 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    42781 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    42841 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    42901 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    42961 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    43021 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    43081 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt
    43141 ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc
    43201 aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg
    43261 ctttaataca gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa
    43321 aaggatagaa cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga
    43381 agagagatgt aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg
    43441 tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa
    43501 attaaaaaca taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct
    43561 tttttacaac ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa
    43621 aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac
    43681 tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat
    43741 ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca
    43801 aacaaaccga tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca
    43861 gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag
    43921 aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag
    43981 atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc
    44041 atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca
    44101 tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt
    44161 gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct
    44221 ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac
    44281 agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc
    44341 gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt
    44401 ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct
    44461 gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca
    44521 gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg
    44581 atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt
    44641 tgctgaatga tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg
    44701 atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca
    44761 ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt
    44821 gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc
    44881 ccaatcgtat cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta
    44941 ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat
    45001 tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt
    45061 tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc
    45121 gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt
    45181 tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca
    45241 tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc
    45301 gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt
    45361 atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga
    45421 ttttctaaat ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct
    45481 tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg
    45541 atctctgcta gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg
    45601 atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca
    45661 cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc
    45721 ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg
    45781 tccaaatttt catctgttga taaaatcata tctttaactt tattgatact tactaggttc
    45841 gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg
    45901 ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt
    45961 gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca
    46021 ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac
    46081 tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat
    46141 tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga
    46201 aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat
    46261 ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg
    46321 atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat
    46381 cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta
    46441 ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg
    46501 acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta
    46561 cagcctttac tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct
    46621 attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt
    46681 cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc
    46741 cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag
    46801 acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag
    46861 caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct
    46921 ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt
    46981 tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt
    47041 ctgcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag
    47101 ctcctaattt ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc
    47161 ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca
    47221 ttacagtatc tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc
    47281 ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt
    47341 ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc
    47401 gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat
    47461 cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca
    47521 aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac
    47581 cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt
    47641 taattgcaaa tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct
    47701 ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt
    47761 cttttttcgc gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct
    47821 aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa
    47881 aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa
    47941 agattacgaa ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg
    48001 tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa
    48061 attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc
    48121 caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa
    48181 agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta
    48241 cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta
    48301 gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc
    48361 ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg
    48421 atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca
    48481 atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc
    48541 gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc
    48601 tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga
    48661 tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata
    48721 gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt
    48781 gccgataagc gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg
    48841 atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc
    48901 ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc
    48961 ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac
    49021 ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc
    49081 gtgcccagag ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt
    49141 ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg
    49201 aagttttttc agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc
    49261 ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt
    49321 gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc
    49381 agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc
    49441 agctgctgct tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc
    49501 tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc
    49561 ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc
    49621 aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc
    49681 aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc
    49741 ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc
    49801 tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac
    49861 ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa
    49921 aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc
    49981 ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg
    50041 gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat
    50101 cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt
    50161 ttctcttttc aataaattta gaacactctc attctaccaa attataacca ttttgccaca
    50221 aataagtcca caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta
    50281 tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc
    50341 gccatctttt tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata
    50401 attaccgccg tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta
    50461 tccttttttc tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc
    50521 tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc
    50581 taataattta tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc
    50641 gatttgttca actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg
    50701 taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt
    50761 tggtgtaacg aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg
    50821 gtgaacgtag ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga
    50881 aatatctaat ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt
    50941 aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc
    51001 acggtaaccg cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc
    51061 gaagacgatt gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt
    51121 ttcgtattct tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc
    51181 tttgatcttc acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg
    51241 cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt
    51301 ttcgatatct tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt
    51361 ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt
    51421 acccacaacg atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc
    51481 gccgtaaacg gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg
    51541 tgtttggata gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc
    51601 tggatcgatt ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg
    51661 tgcttgtcca ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg
    51721 cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag
    51781 ttctttaaat ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg
    51841 ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg
    51901 tttttatcca ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca
    51961 ttagaaaagc caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca
    52021 agttgtttct atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct
    52081 aacatagttt ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc
    52141 taccacttca aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc
    52201 aagattttgt ttgatagcat caatcaattt attcttttct ttttcaaaat agaacacagg
    52261 tagttggggg tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag
    52321 gaaaatcatg gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa
    52381 agactctttc cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg
    52441 cagattttta aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc
    52501 gctcaaaata gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga
    52561 aacatgggcg agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt
    52621 atacgcaatc aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc
    52681 tccttctact acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc
    52741 gccatttcca ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag
    52801 caacggttca tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc
    52861 cattttggct ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat
    52921 cattgtgatt gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc
    52981 gtgatccatg cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt
    53041 gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc
    53101 agacaagaca gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc
    53161 aacgacttct gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa
    53221 aggatctgct acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca
    53281 aaatgaagca gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt
    53341 accagcaaga ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt
    53401 ggtaatcttt gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt
    53461 tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg
    53521 ggttcgcatg actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga
    53581 acgcggttac gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa
    53641 aaagattgac gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca
    53701 ccgtatttca agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct
    53761 ggcatagagt ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac
    53821 cggctcaatc cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt
    53881 tgataaactt cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat
    53941 tttgcttcat aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga
    54001 acgtcttcat ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg
    54061 attgctcgag aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt
    54121 tctgcttttg taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa
    54181 agcaaagaac cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta
    54241 agtactggta cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg
    54301 attttatcca tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat
    54361 gtctctaaga agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa
    54421 acgatggctc cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc
    54481 tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct
    54541 gttagtaatg gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg
    54601 taataattat aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc
    54661 tctgcgctgc gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta
    54721 aatcgttcag tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat
    54781 ttataaaaaa acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga
    54841 ttatttgtcc cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc
    54901 aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag
    54961 tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag
    55021 gccccacctt catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg
    55081 ccttcactca aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg
    55141 tcaaaaagac gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt
    55201 caaaatcgcc ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg
    55261 gtgacgcggt agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc
    55321 ttcaactatt tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg
    55381 atctaaaagg ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa
    55441 aagaaagtca aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca
    55501 gccccaagat ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg
    55561 gtaaaaactt gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa
    55621 ttcaaaacaa gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac
    55681 aggtgtttga tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg
    55741 tactaacaaa agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag
    55801 agttcggttc ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata
    55861 ccatcaacta catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac
    55921 tcacacaagc cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc
    55981 ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa
    56041 gttttcttga acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca
    56101 cacatgtcgt gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt
    56161 tgctgaaagc atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc
    56221 tcatggaagt cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag
    56281 atacattgaa acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc
    56341 ctcaacagct ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg
    56401 aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt
    56461 tcggtatgat tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg
    56521 tagaaaacgc agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg
    56581 tcattatttt tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga
    56641 aaaaggttgt gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt
    56701 ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga
    56761 gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac
    56821 atggaacaca tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa
    56881 taccttcgtt acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac
    56941 gcttgacgaa aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg
    57001 ccagcttttc ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct
    57061 tcgtcaaatt cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa
    57121 gctaaaccat aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta
    57181 taatccgcac tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct
    57241 ttggtcgtat aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct
    57301 aattttatga catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt
    57361 tttcgccttc ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt
    57421 gatccatttt gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag
    57481 cgtgatcaat aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt
    57541 catcaataaa aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat
    57601 ccggcgatcc attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc
    57661 tgttactacg tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa
    57721 gaggaccaat aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa
    57781 acgtttcaaa atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa
    57841 atacataaaa atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa
    57901 taaaaaaagg aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg
    57961 caacttccat tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa
    58021 gaaaccataa atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa
    58081 aagaaacaat aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt
    58141 tcttttgatg tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc
    58201 catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa
    58261 ctcaatcgct tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc
    58321 catattgata aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag
    58381 ccatgctgca ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac
    58441 gcctaatatt ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt
    58501 gtaactatct agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc
    58561 ataaaacagc gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa
    58621 taaaagataa ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa
    58681 attacgccca aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata
    58741 actaatctgc tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat
    58801 tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg
    58861 attactcaag cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc
    58921 aggtgataaa gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa
    58981 accagagtcc ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc
    59041 gagagctttt ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag
    59101 ttcttctgta atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg
    59161 attcactcgc aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg
    59221 aaaaactgaa gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa
    59281 ataaattttt ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt
    59341 taataaaaag taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata
    59401 agttttcagc atgtaatcca cttgtttttg accaatgatc ggcacaaaaa cttccggcca
    59461 tacttcttgt atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc
    59521 catacaccca cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag
    59581 catgatcatg ctatacacac ccaccgacca cggtttcaag aaaatcacaa tctgcataaa
    59641 ttccctaaaa gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa
    59701 gggaagcagc aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa
    59761 ttcatcaaat ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata
    59821 gtaacggaca agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa
    59881 aagcccaaat ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa
    59941 aataggaatg atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc
    60001 aaattgctgg ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc
    60061 ataaagaatc aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt
    60121 ttgtaaaact gatccttttt tagacatcta tattccccac atcttttttc atttattaaa
    60181 actgcctcct t
//
LOCUS       DAFCNJ010000012        58053 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000012 DAFCNJ010000000
VERSION     DAFCNJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.012"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            83..1081
                     /locus_tag="LNE35_000918"
     CDS             83..1081
                     /locus_tag="LNE35_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6910096.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            1105..1389
                     /locus_tag="LNE35_000919"
     CDS             1105..1389
                     /locus_tag="LNE35_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910097.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            1470..1868
                     /locus_tag="LNE35_000920"
     CDS             1470..1868
                     /locus_tag="LNE35_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6910098.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            1878..4421
                     /locus_tag="LNE35_000921"
     CDS             1878..4421
                     /locus_tag="LNE35_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6910099.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            4443..6785
                     /locus_tag="LNE35_000922"
     CDS             4443..6785
                     /locus_tag="LNE35_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910100.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            6801..8975
                     /locus_tag="LNE35_000923"
     CDS             6801..8975
                     /locus_tag="LNE35_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HBK6910101.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            8965..9387
                     /locus_tag="LNE35_000924"
     CDS             8965..9387
                     /locus_tag="LNE35_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK6910102.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            10330..11241
                     /locus_tag="LNE35_000925"
     CDS             10330..11241
                     /locus_tag="LNE35_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HBK6910103.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            11728..11994
                     /locus_tag="LNE35_000926"
     CDS             11728..11994
                     /locus_tag="LNE35_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910104.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            12066..12407
                     /locus_tag="LNE35_000927"
     CDS             12066..12407
                     /locus_tag="LNE35_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910105.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            12462..12881
                     /gene="ssb"
                     /locus_tag="LNE35_000928"
     CDS             12462..12881
                     /gene="ssb"
                     /locus_tag="LNE35_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK6910106.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            12958..13254
                     /locus_tag="LNE35_000929"
     CDS             12958..13254
                     /locus_tag="LNE35_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910107.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            13269..13700
                     /locus_tag="LNE35_000930"
     CDS             13269..13700
                     /locus_tag="LNE35_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBK6910108.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            13882..14187
                     /locus_tag="LNE35_000931"
     CDS             13882..14187
                     /locus_tag="LNE35_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910109.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            14591..15079
                     /locus_tag="LNE35_000932"
     CDS             14591..15079
                     /locus_tag="LNE35_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK6910110.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            16413..16715
                     /locus_tag="LNE35_000933"
     CDS             16413..16715
                     /locus_tag="LNE35_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6910111.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            16708..17736
                     /locus_tag="LNE35_000934"
     CDS             16708..17736
                     /locus_tag="LNE35_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBK6910112.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            17792..18010
                     /locus_tag="LNE35_000935"
     CDS             17792..18010
                     /locus_tag="LNE35_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910113.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            18081..18896
                     /locus_tag="LNE35_000936"
     CDS             18081..18896
                     /locus_tag="LNE35_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910114.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTESKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            18960..19145
                     /locus_tag="LNE35_000937"
                     /pseudo
     CDS             18960..19145
                     /locus_tag="LNE35_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
     gene            19719..19982
                     /locus_tag="LNE35_000938"
     CDS             19719..19982
                     /locus_tag="LNE35_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910115.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            20316..20687
                     /locus_tag="LNE35_000939"
     CDS             20316..20687
                     /locus_tag="LNE35_000939"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK6910116.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            20867..21289
                     /locus_tag="LNE35_000940"
     CDS             20867..21289
                     /locus_tag="LNE35_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910117.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            21434..21679
                     /locus_tag="LNE35_000941"
                     /pseudo
     CDS             21434..21679
                     /locus_tag="LNE35_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21788..22744
                     /locus_tag="LNE35_000942"
     CDS             21788..22744
                     /locus_tag="LNE35_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910118.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(23580..24251)
                     /locus_tag="LNE35_000943"
     CDS             complement(23580..24251)
                     /locus_tag="LNE35_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6910119.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            24568..26199
                     /locus_tag="LNE35_000944"
     CDS             24568..26199
                     /locus_tag="LNE35_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK6910120.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            26202..27281
                     /gene="uxuA"
                     /locus_tag="LNE35_000945"
     CDS             26202..27281
                     /gene="uxuA"
                     /locus_tag="LNE35_000945"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK6910121.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            27355..28782
                     /gene="uxaC"
                     /locus_tag="LNE35_000946"
     CDS             27355..28782
                     /gene="uxaC"
                     /locus_tag="LNE35_000946"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBK6910122.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            28866..29513
                     /locus_tag="LNE35_000947"
     CDS             28866..29513
                     /locus_tag="LNE35_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBK6910123.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            29642..31018
                     /locus_tag="LNE35_000948"
     CDS             29642..31018
                     /locus_tag="LNE35_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6910124.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            31029..31823
                     /locus_tag="LNE35_000949"
     CDS             31029..31823
                     /locus_tag="LNE35_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK6910125.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            31836..33602
                     /locus_tag="LNE35_000950"
     CDS             31836..33602
                     /locus_tag="LNE35_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBK6910126.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            33630..35438
                     /gene="uidA"
                     /locus_tag="LNE35_000951"
     CDS             33630..35438
                     /gene="uidA"
                     /locus_tag="LNE35_000951"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBK6910127.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            35610..36011
                     /locus_tag="LNE35_000952"
     CDS             35610..36011
                     /locus_tag="LNE35_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6910128.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            36005..36250
                     /locus_tag="LNE35_000953"
     CDS             36005..36250
                     /locus_tag="LNE35_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6910129.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(36574..36795)
                     /locus_tag="LNE35_000954"
     CDS             complement(36574..36795)
                     /locus_tag="LNE35_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910130.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(36957..37029)
                     /locus_tag="LNE35_000955"
     tRNA            complement(36957..37029)
                     /locus_tag="LNE35_000955"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36994..36996),aa:Lys,seq:ctt)
     gene            37105..37335
                     /locus_tag="LNE35_000956"
     CDS             37105..37335
                     /locus_tag="LNE35_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6910131.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            37379..38548
                     /locus_tag="LNE35_000957"
     CDS             37379..38548
                     /locus_tag="LNE35_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6910132.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            39122..39691
                     /locus_tag="LNE35_000958"
     CDS             39122..39691
                     /locus_tag="LNE35_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBK6910133.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            39678..40538
                     /locus_tag="LNE35_000959"
     CDS             39678..40538
                     /locus_tag="LNE35_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6910134.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            40728..41432
                     /locus_tag="LNE35_000960"
     CDS             40728..41432
                     /locus_tag="LNE35_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6910135.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            41437..43272
                     /gene="walK"
                     /locus_tag="LNE35_000961"
     CDS             41437..43272
                     /gene="walK"
                     /locus_tag="LNE35_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBK6910136.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            43269..44585
                     /locus_tag="LNE35_000962"
     CDS             43269..44585
                     /locus_tag="LNE35_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910137.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            44586..45455
                     /locus_tag="LNE35_000963"
     CDS             44586..45455
                     /locus_tag="LNE35_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBK6910138.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            45510..46319
                     /locus_tag="LNE35_000964"
     CDS             45510..46319
                     /locus_tag="LNE35_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6910139.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(46422..47051)
                     /locus_tag="LNE35_000965"
     CDS             complement(46422..47051)
                     /locus_tag="LNE35_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBK6910140.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            47221..48513
                     /locus_tag="LNE35_000966"
     CDS             47221..48513
                     /locus_tag="LNE35_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6910141.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(48589..49251)
                     /locus_tag="LNE35_000967"
     CDS             complement(48589..49251)
                     /locus_tag="LNE35_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6910142.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            49476..49760
                     /gene="groES"
                     /locus_tag="LNE35_000968"
     CDS             49476..49760
                     /gene="groES"
                     /locus_tag="LNE35_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBK6910143.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            49811..51436
                     /gene="groL"
                     /locus_tag="LNE35_000969"
     CDS             49811..51436
                     /gene="groL"
                     /locus_tag="LNE35_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBK6910144.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(51525..52673)
                     /locus_tag="LNE35_000970"
     CDS             complement(51525..52673)
                     /locus_tag="LNE35_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6910145.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(52775..53359)
                     /locus_tag="LNE35_000971"
     CDS             complement(52775..53359)
                     /locus_tag="LNE35_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBK6910146.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(53486..53917)
                     /locus_tag="LNE35_000972"
     CDS             complement(53486..53917)
                     /locus_tag="LNE35_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK6910147.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(53935..54255)
                     /locus_tag="LNE35_000973"
     CDS             complement(53935..54255)
                     /locus_tag="LNE35_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6910148.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            54555..54695
                     /locus_tag="LNE35_000974"
     CDS             54555..54695
                     /locus_tag="LNE35_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910149.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            54993..55250
                     /locus_tag="LNE35_000975"
     CDS             54993..55250
                     /locus_tag="LNE35_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HBK6910150.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(55221..55529)
                     /locus_tag="LNE35_000976"
     CDS             complement(55221..55529)
                     /locus_tag="LNE35_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910151.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            55607..56353
                     /locus_tag="LNE35_000977"
     CDS             55607..56353
                     /locus_tag="LNE35_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBK6910152.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            56368..56571
                     /locus_tag="LNE35_000978"
     CDS             56368..56571
                     /locus_tag="LNE35_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6910153.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            56676..56975
                     /locus_tag="LNE35_000979"
     CDS             56676..56975
                     /locus_tag="LNE35_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910154.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            56975..57157
                     /locus_tag="LNE35_000980"
     CDS             56975..57157
                     /locus_tag="LNE35_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910155.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(57163..57639)
                     /locus_tag="LNE35_000981"
     CDS             complement(57163..57639)
                     /locus_tag="LNE35_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910156.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            57692..>58053
                     /locus_tag="LNE35_000982"
     CDS             57692..>58053
                     /locus_tag="LNE35_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBK6910157.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIE"
BASE COUNT        20288 a         8678 c        11565 g        17522 t
ORIGIN      
        1 gagggggcaa gtgtggttgt ccacgaagta gtgattgtgg acaaaatacc gaaattattg
       61 ttagaacggt atggcatttc tcatgagtaa tgcaaaattt aatccacaag cgttccaaac
      121 taagccaccg aaacaacaga aggaaaagaa acaaaagaaa cgttttttgt tccaaaaaaa
      181 gaaaacagaa ccgtcagatc gaatgatcca attttcgcaa aaaagtgcca tgagtgtatt
      241 ttcaggcgct tttttttgtt tgtttctgtt aatgggtgtc gtgctcctcc taaactttgg
      301 acgagtggat acattaacgc gtttagcagt gaaaaaacaa gtcaatccag aaacattgtt
      361 aactgaagtc aaccaaacct tgtcttctac ggaacaattg aagtacgagg gacaacattt
      421 tattgaaagg ttatttactt attcgccaga aaaacgaaac gaatgggagg cttccgtcaa
      481 tcattattta gcacaaggat tggaagcgtc tgatctaggc tttgtaacga cggctaccaa
      541 tcgagtagcg aaagatgttc gctttattaa actagaagaa atcgatttga cacaagcaat
      601 gtatcgccta ttttatgagg ttacttttac agaaggagaa cattggaaaa gcagtcaact
      661 ttcttttcca gtcagttatc aaaacaatgt tttaaaagta cttgatggcg ttcaattttt
      721 gaatctttct gagagtaaga ataccaattc tgtgaactat caggaaaatc gattttattc
      781 ttctgggcaa gcggtcactg aaagcgaaga aatcaagctg gaagcatttg tgaaacggtt
      841 ttttgagtta tatgtcaaaa atgatgaaaa attggcattg atttctaacg aaaaagggtt
      901 agaaggcggc gaattgatca atgtacaact ggatcatgca gtaaaaaata aagaaggaaa
      961 ctactttttg caagggaagt atcgtttcta ttttgagaaa ggaaacgagt tccaatcaaa
     1021 cttttcaatg gaagtaaaaa caagtggcga tagttatttt gttactacgt taaatgaata
     1081 aaatgaactt agaggagaat ggaaatgaaa ctttttgatc ttaaatcgtt atttgatgat
     1141 gtatctggac aagtcatttg gctattggca atcgttgcga ttgttctaat ggttggtgct
     1201 tatgcgaccc aaggctttgc gcgggcagcg tttgccatta ttggcgtctt tattttgata
     1261 atgctcgtct tattgttatc gaatgccgtt gatattggtg aatggctaaa gaacattatc
     1321 tttattaagg atagtgaaga gaacgtgggg gtaacgcaaa tgattgtgcc ctggatacaa
     1381 ttatcataag aaaaagacaa actgcaaaag cgatcagaag attagaactt ctggtcgctt
     1441 ctttttttgt aaaaggaggt agagtgaaca tggaatacaa ctatacaaag gagtttaagc
     1501 accctcacaa aatttacggg attaaaggac aaaaaattcc ctggatttct aatggcattc
     1561 gcatggaaca attagtcata ggtggggtat ttctcttcat tcttttgatc ctatttttga
     1621 ttgcgtatgt gaatcaaatt agtttcttgc agacactgtt tcgaaagtcc tggttgatta
     1681 tgatttttgg gattggtgtt ctcgtttgga cattgttttc aatgaaatgg gacaataaac
     1741 actttttcga gtatttaatt gggcgaacgt cctattatca gaaacggaaa caacgctttg
     1801 aacatgaaat gtatgtgccg tttttccatg aaaaagtgac gtattcccta cacaaatgga
     1861 aggagtgagt cgagtaaatg gcgtttttgt ttcttttggt cgtctttatt atcgggattt
     1921 ggcagttcaa aaagaaaaaa acgaagcttc ctcgattaga atttccgata gacgccatta
     1981 tccgaaattt tgttttgacc aaaaatgatg atgtgtgggt aggctatcaa ctttcttccc
     2041 aagtatttcc tttaaatgac attgattttt tcaagaacta tcttcaagat ggcgaaggca
     2101 tacttggaca tgatgaatat gactatcatt ttatcaatat tccacgtcaa tttaacctag
     2161 atacgcacat tgaagaaacc attcgagatt tggtaaaggg agaatgcgca gatcttggga
     2221 aagtgtactt tgaacaggca ggaaagattt taaaagacga agtgcaatta aacgagtatc
     2281 gaacgtatct gtttgtgcgt ttcactacag aaatgaaagt gtctgatccg atcgaatatt
     2341 ttcaattgct taagcatgtt tttaaaaagt ttatctatcg tattacggga caacgagttc
     2401 ctataagtag tgtgttacca acgtatgaaa cattagaaaa ggagctctat acggaattgt
     2461 ccaattataa gccgatagag cgcttatctg cccaagagat tggacaaatt tattattatt
     2521 ttttccatcg atcagaagaa aaattgcctg aaagacagct tacgccaatg gaaatttgcg
     2581 agggcgtcat ttcgaatgaa gcaggataca ttgcgattga acagttgaat caaacgcatt
     2641 attcgacgtt tttaagtcaa acggacgctc ctttagccat gtatgggagt agctttattc
     2701 aaaatcttca ggatagctta tcttttccaa ttgatacgca tattcgactt cgatttgagc
     2761 attcaaaatc tgatgaacga aaaattaata aaatggctgg acggatttat gaacaagaaa
     2821 aagaatatga gaccgttgac ggcattttag attcagacga tgtattaaat tttggtacag
     2881 aacggttaat ggatgtaaaa gataagctga agcgcaaaga aaaacgattg tgttattcta
     2941 ccgtcacgct ggttgtatct gcttcttcaa aagaagaatt ggaagaacga gtcaaagata
     3001 ttgaatttgc gattgagtct acagatttca aattttatcg atcggtagcg gatcagctaa
     3061 cgttgtttaa tcaatgctta attggaagta cgcaacgttt tcgtagttac gagcaggtcg
     3121 ctacgacagg ttttgtggca gattatggct tggacttaaa gaaagaagta ggaaataaga
     3181 aaggatttcc tttaggacgg attgttattg caaaaaagtt taaatctgcg aaagaagcgt
     3241 tagcgctatc tagtaaacta gtatggtatt atcccaattt aacaaagaaa agtattgaag
     3301 gcgcggaaca taccaatgga aatacgttga ttattggtcc accaggtaaa ggaaaatcgg
     3361 tccttgtaaa atatattttt ttatggctga tgtttttagg tcaaaaaatt ctttatattg
     3421 atccgaaaaa tgaaacccaa aagttctttt taaaggcact tgaaaaattc ggacacatac
     3481 cagagtttgt ggctatgtat gaacgaatta attttatttc gttatcttca gatgaaagct
     3541 gtcgagggat gcttgatcct ctattgtttt taccaaaaga agaggcaatt caaaccgcta
     3601 aaaatgtgtt aggaatgtta ggagaagtag ataccaatcg agaaacagcg agccacaaga
     3661 aaacggtgat ccaagacgcc atcgatgtag tttacaccat gccaggtaag cattcgttaa
     3721 gtcaagttat cgaagaaatt cgaaaaagtg acaaagaact agctaaattg attttagggc
     3781 atagcgttgg tttaggaaag attctcattg ggaatgagca cagtacgccg atacgttttg
     3841 attcacaaat caacgtgtta gggacacaag gaatacgcat tccgacacaa aaagagattg
     3901 attcgggtcg attaaatagc gaacaacttg caggaatggc aattatggaa gtcattatga
     3961 agtttacgta tatcttttct acaaacaaag aagaagacgc tgcgattatt tatgacgagg
     4021 caaaagggta tgaagatacc gcacaaggaa gctatttgat tgatgatagc ttgcgaaaag
     4081 gacgtgcgaa taatacggat atttaccttg ttgtgcaagc gtttaaagat tacgatcaag
     4141 aagataaaaa agagttaatt tcttacaaat ttgcgtttcg tccgaaacaa aaagaagcac
     4201 gagagaaact attgtccttc tttgatatgg aagtgaattc agccaattta gaaatgatgg
     4261 aacaattaca aacaggaacc tgtttattcc aggatcattt gggaagaaat caaccgattg
     4321 ccattgatgt gttgtttgat agttggttat tggctgtttc ttctactgaa aatcaagatg
     4381 aaaaaattca agcaatgtta gaaaatgaaa aacaacgata aaacaaaaaa gaggtggttt
     4441 ttttgcgaaa aaaatctctg aaatggtgga cgctcctttt attagggggt ctttttttct
     4501 gtctacaagg ggctactgtt tctgccgatc aaatgaatga tggtgcgggt gtcactgaaa
     4561 ccgatcgttc tgaaggagag aatgccaacg ataaagtaga agtaaaagaa gtaacgactg
     4621 atgtggatca attgtataaa aattacaaaa agaattcgtt tgaattgatg accaatgact
     4681 atgagtcaat gaaccttaaa aaaaagctat cgtacaatgt tgcaggagtg atgaaaaatt
     4741 ttgtttggag tggcgtaaaa atgttaggag aattcaatgc ttcaatggtt gaatttctct
     4801 ttgaattaga tattatttcc gccattcgag aacccattca aaacttaaca caaaaaattg
     4861 cggacaatat gttaagtatt gcaggaacta ttggaatcag tttttcggca attatgatgg
     4921 caatcaaata tgctggagag gctcgaatga aacaagcact ccgtgtcttt cttatgacga
     4981 tccttatttt tactgggtta agcgtattaa aagattctgg gacaaataag aatctttttg
     5041 atgttctttt ttctttagat tctacagtgg aaacggcgtt cgtgaaggtc aatccagtgt
     5101 taacacctga agaagaaaca aattatgaaa caaaagaaac agaagaggta acagcggata
     5161 aaaaagtgga aacggcaggc aaattgatgg cggctcgggt cttttatagt aatgtttatg
     5221 aaccatatct gttgttaaat tatggcacaa ccaatgtaga cacaattcgt gagaagaaag
     5281 tcaaatatgc tggaaaagaa tatgatcgaa tcaatctttt attggacaat gatttaggta
     5341 caaaaatagg ggagaaaatt catgacaaag tcacagagta tgaagcagat acactgaaaa
     5401 acaagaacat acaatattac aatagttgga aaaatacagc atttgccttg ttttattttg
     5461 tcgtgaatct gattcaaacc gttgtttatt ttgtcttaag tatgtttcga ttgattattg
     5521 gaactatgca actattgtta gtaccgatgt tgccgttctt acttttttta ggattgtttc
     5581 ttgtcacaat gaatgtgttt ggtaactatg ccaaagtttt tggtattttg acgtttttaa
     5641 aaggaatgac aggttttgca tgtatccttt tttcttcttt tcttagttac gggttccaat
     5701 taagtagtac acaagacgat ccatgggaaa aaattctctt gatcattgtt tatttgtttg
     5761 cgccgttggg aatttatttg ttccgaggct ttttaggcag tctatttact ggaagaatgg
     5821 gtgttcgagg agcgttagct tttgcgggaa atccatttgg ggcagaagca aaattgagaa
     5881 gggaagccaa acaacggttg aaagaacgca aagaagcgag aaaagcacag aaacaaaaag
     5941 aaaaagaaaa cgcacggaag agacaagaac gtcaacgaaa acgtgggggt ggctcagacg
     6001 ttcgtatgcc acaaacggcg aaacaagcca cacgaagtgc ttttcggcgt gaatccgcac
     6061 aacaacctcg tcataaagca ccaaatcgta tggaacgtat ggcacaaaaa gcaagagact
     6121 tgcatgatca agcgaaacaa gcagaagagc aaagtcaaga acgtatgcaa cgaacagcaa
     6181 tggagaaaca ggcaagaaaa acagccgatt tattacaacg agaattgcaa ggcaatccta
     6241 gtctaagaga aagaagtaga aaaggtgcgt ctcgtagtcc gtatcgcaat cagggttctg
     6301 caatggtggt gtcattagcg aatccaaagc aaaaacacaa gtcttatggc cagaagcata
     6361 cgcctagaac agaattgaac gatcgaaaac gtcgtcaggg acaaacaagt cgtcgtcgga
     6421 aggaggcact tactcccgta acaaaaacaa gttcgatgaa aacccggaca gttgcaggac
     6481 aaaaacatgc ggtacaaaaa ccactaagaa aagggaatgt accacgagca acaggttcta
     6541 ccacaccgaa atctgttcga cgtccagagg gggttcggga acatatgtca gcagtcaatc
     6601 gagtaacagc acaaaccaaa gcgccttcaa ccgtatctaa agcatactca accatgaaac
     6661 aaacaaaatc accaaaacaa gcaccgaaga cacggagtga gtttcgagca actcaagcaa
     6721 aaatgaaacg aaaacgagaa aaacaaccga tttatcgaag agaaatgaaa caacgaaaac
     6781 gataggagga aatcgatagt atgatgaatg caccaaaacg accgacacat tttgagcaaa
     6841 tgttttctgt cgatcgcaaa agaaaacaag aaaaagaaac gccaaaaagt cggtttcaac
     6901 aactttattt tatcgtcact ttattaggtg tttgtctata tccatttttt attgtaggca
     6961 gtattccttt agcgattgtc tttatgatag ataaaaaaga gacacgtgca catatttatg
     7021 actttgagta tgagagcttc ttaaaacgaa gaagctctct tttttttatt tttgcgttag
     7081 gttttacgct attaaatcta ttttgtttta tttttgttct tcctagaggg tatttgtcgt
     7141 gttacttatt atttccatta aacttgtttt ctcatgcgtt gcagtttaat ggacaaacga
     7201 tttttgccct ccttttaggt gggacaggtg caggaagttg tttgattgcg tatgcgtctt
     7261 ttattgaaaa aagaaaagtt gtttcaaaga aagaacggca aacagctttg ctaaatagta
     7321 aagaatacaa ggaacgtcgg aagaataaat ttcaggagtc ccaaaaatat acagcagagt
     7381 atcagcaagc gtaccaggta gcaattgtca taaaagacgt tgctttgcgg aataaacaac
     7441 tggcttcatt agccaaagtg attctattag gaacagatga gtatgggctc ccttatgtga
     7501 tgaactttag tgaattgaac caacatgtga ttattccagc gactacaggt tcaggaaaaa
     7561 cgacggtgat ccaattacta gcagaacatg cgtgtaagtt tcgtatcccc gttatcttag
     7621 tcgatgggaa aggagcagga gagactttac aagcgttaaa agaagtggca gaggcataca
     7681 atcgcccgat tgaagtcttt gtggacggtg gcgatttgcg ttataatcct gtaaaaaatg
     7741 gcaatgatat tgccattcga gataaattaa ccaatctagc agaaacagaa agcgtgtatt
     7801 attcgacagc agccaaagcg ttattacagg taacgattca gcttttagac gagttccaaa
     7861 agagccataa aattgaaaga aatctagttt ggttacaaca ttatttattg ccacgaaacg
     7921 tcttacattt gtttgcggat cgtattcagt tgaaaaatcc agatttattt aaagaaaaag
     7981 tggtcgtaga aaagaagaaa accacgaaga aaaaaacgaa aaagaatgaa gcgatggaca
     8041 aaacagaacg tgattttgcc gatgtggaac agttaagtga agagcgaatc gttgaacatg
     8101 atcgtaaaac cgttgactta gaaacgtatt atctattatt aaaagaaaac atttctctca
     8161 tgacagaaat cgagcaaacg ttgtttaaac ggttgtttgt tcgttacgaa cataagaaga
     8221 acccgtttta tttgtatgcg acttctgaga atttacagac caatatcaat atgttattgg
     8281 atagtgagct tggtgaactc tttgatactt ctggaggaaa aagagaatta gatattcaag
     8341 aagttgttaa agaaggaaag attgtttata tctcgttaaa cggattgatt tatagtgagt
     8401 ttattcggac attggctcaa atgcttgtgg gagatattaa tttctttgcg tcagaagtgt
     8461 ataaacaagc aggagaaaca gccaatcgct cgaaagtcct agtaatattt gacgaaccag
     8521 cttcttatct aaatgataac cttatagact tagtcaataa gggacgaggc gctggggtgc
     8581 atgcggcgta tgcccctcaa acgcttgcgg acattgatag aattgatcct gtccttcgaa
     8641 aacagttgat tgggaatgtg aatacgttct ttattgggaa gacaaatgac ccgacagaaa
     8701 ttgattattg ggcagattta atggggacag aagaagacat tgaattaacg gaaatgacga
     8761 cacaagaagc aggttattct gatatgggaa aggttgattg gacaggagaa agaggcacga
     8821 aacgagacgt agaccgcttt aaattcaatc cgaatcgcat tcgagcattg agaacaggcg
     8881 aatttgtggt gtatcgaaca gctgaagacg tccaggaacc accgcgagtc gtctatattc
     8941 ggaaaccaaa tttggagaaa caatatgcga aataagaaag ttattgttag gatacttatt
     9001 gttagtttcc tcttatttgg ttgttttaaa ctgataaaaa ctaacgaaga atcaaatgga
     9061 aaactaatca atagtaattt agaagaagta ctagaacttc aaaaaacgaa tcaatcattt
     9121 tttgtgtatc tagcaagaaa tgattgtaaa aaatgccaag aagttgaatc tttcttggaa
     9181 agatacgaca aaaaattctc taggaatgta tatcgaattg aaacaagaaa agaaccaaga
     9241 caagaagaac ttcaaacttt tttacaacgt taccatgtta aaagtgtccc aagttttctc
     9301 gtgattcatg atagaaacgt caaaaaaatt gagagaaaaa cattcttttt tgaaaagaca
     9361 ttgaatcaat ttcttcctaa tttttagtga aaattgaaag agaatacgtt gaaaaaaaca
     9421 aaaaattgtt atgagttcaa atttttttcg atcgtatttg tttttctttt gaaacaaatt
     9481 cactcatttt ttgagcaaaa atgtttcaaa atttgaacaa aaaccaattg aaaattgaag
     9541 caaatttcac tcgaattgaa tcgattttga gctaaaatca atagaaaaaa taaacgtatt
     9601 ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta gtaaggatta tttgagggtt
     9661 atttagagat tatttaaagg ttatatgtaa taagtggggg gagggctgtc ggcgcttggt
     9721 atgaaaggca ttctccgact tccaggtttt tgaccatttt ctctcaacgt ggaaaaaccg
     9781 ccacttatga gagaaaatgg tcaaaaacct aagcgggaag tctccgaata acataccaag
     9841 cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat aaaatttaaa
     9901 agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc cccctaaccc
     9961 cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac gccccagtgt
    10021 tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa gtgtagagga
    10081 aacttgaaga cttgttcgaa cagaaacttg cgagagagcc ggagtaaaac aagtgtagag
    10141 gaaacttgaa gacttgttcg aacagaaact tgcgagagag ttggagtaaa acaagtgtag
    10201 aggaaacttg aagacttgtt cgaacacaaa cttacaagag aggggagtaa agcagttgca
    10261 gaggaatcag gaagatttgt tcgaacagaa acttgcaaga aagcattatt aaattgaagg
    10321 tgatgaaata tgaagtattt attgcatggc tattttattg attattctac tcgcaatagt
    10381 agtactttag cgattctatg tttgcttcat gaattgagag tggttactag aaaacagcta
    10441 tacaaactat tgaattttga cagatcaatt agtaaacaag gagtaacgaa tgctttagtg
    10501 agattatatg aaaaacagtt aatcgatcga aaaaaatatg gaacagaaat gtattattat
    10561 ttgacgaaag agggacatca aagcataggt gggtattata cgttgccgaa agttcctgaa
    10621 tataatttga atcaccattt acaaattaac aattatttga ttaaaggaat ggcattggca
    10681 aaagaccacc cacatttaaa acggattatt tcagaaagac gtaccgtcta tgaaacgaag
    10741 gatttaaatc ctaagacaaa aggaagaaaa ttttttgtgc cagattttat ttttcgtttt
    10801 cgagacaaag aaaatcatga gatcaattgg tcctttgaaa ttgagctaac gttgaaaaca
    10861 cgtcgtcgct accgtgaagc gatttttccg aaatatattc gtgagttgcg aacacgtcct
    10921 gaatcacatt taatttatgt gacacctagt ggcgcaattc aagaagaact tcatgcgttt
    10981 aagcgtattt ttttcaagcg cgaaactgaa aaagatgcga gtcaacaaga attgtttgag
    11041 cggttgcata ttttttcagc tttagagttt gaagagggat taaaagcatt attagagaat
    11101 gatccagcag tcaattggcg gacagtagaa cgagaaaaaa tttatccgaa tggcgctgtt
    11161 caaagtcgtt tgaatttatt tgaagaatca actgaaaatg aggaaaaacc aaaagtgaag
    11221 tttacgcctg aagtattgta aaagagggtg ttcgcttcgc tctttttttg tgccagtttg
    11281 ctggcaccgt tcagacgcaa gcgtctgaac cggctcgttt ttaggaagga ctaacttgtt
    11341 tgatttatcc gttgtcaaag aagtggacgt tcgagagtcc ctaatcgtat agcagtcatt
    11401 cggcgagtat aacctaaaac ataacctgcc aattgccaag ggtaaaatgc acgctgcgcg
    11461 cccttggcaa tcgtcagaac atgttttcga gccggttatg ccgaacgacc gctatacgaa
    11521 aagggcctct aaacagaagt ccactttgat ccttgacaac tgaaacccaa aatcaaacaa
    11581 gcaggccttc gtctaaaaac gagccgaatg aagaccttgg gggttgcacc cccaaacccc
    11641 cgtgcccccg acacactacc gaaaaggtgg gggtgggtgg cgaaaaaatc aaataaaaca
    11701 ttctattcaa gaaaggaaga aacaaccatg acaactaaaa acgagcaact gaaagaacag
    11761 gcaagaaaag agttgcaaga aagaggtttg attattgagg ggagctttga aggagatttt
    11821 gaaacctata ttggttgtta tgcaagaccg ataaacaaac ctacagcctt agacccaatg
    11881 aacgaacaag aagtaaaaga acaagaaaaa catgcagtga atggttttcc gcaagatttc
    11941 actgaatggt atgagtggga aattaacaac gggaagcttg aaaacttctt ataaaagtca
    12001 gatgacgtgc aaagagaatg attctctttg tacggtaaaa atataaagaa agaaggaaaa
    12061 agaaaatggc aaaaaacaca ccttatcaag taggagacat tttttatagt agttggggct
    12121 acgaacaaac gaacgtgact ttttggcaaa ttgtgaagtt aacagaaaaa acagcatggt
    12181 ttcagcctat tcaaaagaaa acggtcgagc attattcgtc catgagtgga aaagttgttc
    12241 caatgaaaga caagtttata gagtatcctt tagcaaaaac taaagacagt attgtaaaaa
    12301 aacgaattga tccagaaacc tatcctgata tgttatgtgg ttatcgtagt tgggacacgt
    12361 ttcatagata taaaaatcaa gctgtttatg aaagtagtta ttattaaggg gatagtttcc
    12421 cctttcaaaa ataataaata gaagaatgga gagaaaaaat catgatgaat tcagtgaatt
    12481 tagtaggacg gttaacaaaa gaagtggatt taaaatacac accaaaaggc aatgcgacag
    12541 gcacatttat tttagctgtt aatcgaaatt atacgaatgc aaacggcgag cgtgaagcag
    12601 attatatccg ctgtgtcatt tggcggaaag ctgcggaaac tttagcaaaa tatactggaa
    12661 aaggaagttt gattggcatt aatggacgtc ttcaaacgag gagctatcag aatcagcaag
    12721 gacagaccat gtatgttacg gaagttctag tgacagattt ctatttgtta gagagtaaag
    12781 aaatcaatga gcaacggtca aaatcaatgg cgaataatca aaataattct tcaattccag
    12841 gaaatgagat tcaaatttca gatagttatt tgccctttta agtgaaaaat aattttgttc
    12901 tatacctgat ttcttcaggt atagaacaaa aaataaatag agatgaggcg attttttatg
    12961 aaaatcattg acagttacga tcaacgaacc atgcaatatt tgggctcgtt tgattataca
    13021 gatgaaaata ttattaatta tgttgcaagt ttacctcctt atcaaagcgt tcgcttggta
    13081 gattttgcaa gtgatgaatt ggtattaaca acgatgggca actttttaga ttatgtcgga
    13141 gacaaaaaat ggttggaaaa aattttgtct aaattgattg cgaaacaaac aggagaagaa
    13201 cccataaatg cgattcaatt gttcaatcga tatgaacaag aaaacaatga ttaaagagga
    13261 gagaaaaaat gaaaagatta aaacgaagta tttttattgt gctagcattg attgattttg
    13321 ttgggacagg ttgtgaagca attgacgttc aaaaagctcc cttatcggaa ataaaacaag
    13381 caataacaaa acaagatgaa gaacaaccgc taaatacaca agtagaaata gtgaatcaaa
    13441 ataaaccgag tttttctgaa gaagattttt cgttagaaaa tggaaccagg caattttttt
    13501 cagatttgga tcgtttaaat cgggtaggtg tagcaagtgc acttttgcat aaaagcagga
    13561 tgccaacaga agaacgagac cgattataca ttaagccaac tggctggaaa caaaagaaaa
    13621 tgaagaatgg cgattatctt tataatcgtt gtcatttgat tggctatcaa cgtgcgacaa
    13681 gtttccccat aagtatgtaa tacgaaacgg gaggattaat cttaagatta atcacaacta
    13741 acctatttga cagttggaat gaaagctatc atgtctgctg aatcaactgg tctgatactc
    13801 ctacatgcaa taaagtctac taggaagtta ttgtatgaag ctaggtaaag gcggcagaac
    13861 aatttctaag tgtggaatca tatgaagttg gatatgccgg gacgctataa attactcagg
    13921 gtgagactgt ttttaattaa actgacgaac ttgcgaatgt acgggtctat aaccgactta
    13981 tattcgaaag atataagatt gcgaatgcaa agaacattat cgaaaagtaa gaatgttggt
    14041 catgaaattc gagatggaag actagcggtg tatctgtcaa gagttggtaa aacatgtttg
    14101 attgattcag atgagagtgg gaatgcgaca aaacgtttta cagaagaaat agagcaacta
    14161 gcagaactga atatacgata tttttaaata ctttagcgga gaataaaaaa atcctctaca
    14221 gaaaactacg gagggcaaca tgtgctattg tttcaacaat acatttttaa tttttaccat
    14281 aacaaaaaag tacttgaaaa aaaacagaaa tatgttaaat taaaaatgaa aaaatggaag
    14341 cggttactta ttcttttgaa tctaagataa aaattcaaaa gaataacata acccaaatat
    14401 gattatactt gagattctaa atatttatcg aaaaggaatt tgttttaagg aaaaatgatt
    14461 ctatggacgg ttattgattt atatttacca aatggctaat aaaatagtat ttcttatatt
    14521 gtatagtttt tgtttttatg tgaaaacatg agcaatagaa ttgtactagt aggataagga
    14581 tggatggaaa atgaaaaaaa ctatttttgt tttagcgatt tttatatgtt ttgggctact
    14641 tttggtaggc tacttgttgt ttaattctaa tgaaatgaga actatttcta cgaatgactt
    14701 gttaaaaaaa agtgaattaa aggataaaaa aaaagaatct cttgccttta taatgtttac
    14761 tcaagaagag tgtgagtatt gtatagcaat aaatccaatc atagatagtg taaataagga
    14821 aactttgaat aagataaaga aatatgatat atctgtagat tttcaatcta aacaaaagtg
    14881 taaaattaag tttgttccga ctattgttgc atatagtaag aataaagaag tggggagaat
    14941 tgacaatgaa ttagataatt tgtatcgaaa aaagcaagat ggttcaattg taatagacaa
    15001 gaaaaaaaca aagacattgc ttatagattg gatgaaaaaa atggataaac aaggagaata
    15061 tttgtatgga gaagattaaa cagacatttc cgttaattaa taaatattgg aataagaaaa
    15121 aaatttttat attttttatt acgtttctgc tgtcatcttt attttctcta attttcccgt
    15181 atctatttag taatgttatt gatttatatt tagagaatga gttatcacag aatattgtgc
    15241 gaatagtaac gattcttata tttcagttat ttttagtctt aattaaagaa gtgtatggat
    15301 atataaaaac tatttatgca tttgaagttg atttatcaat gaaaattgat tttcttaagg
    15361 ttttacaaaa aaaaaatctt tttttttcgc aaacgaagca ttcaggagaa attcaattta
    15421 ggatgttttc ggatatttct actatcgtaa atactatctt tattttttct gttgaattac
    15481 ctgtgatagg cgttttagtt cttgtagcgg tatttattat aagtaaagtg aatcttattt
    15541 ttctgatttt ttcactaata atgatgggag ctatggtgtt acagtataat ttatttcaat
    15601 taccgcttaa gaaaacaatc gagaatacaa aaagtgttaa tcaaagtatt acaggagaat
    15661 tgaatgcaca ttttgatcaa ttagaaatta taagcgcata taattttgag aatatctcta
    15721 ttttaaaatt cgattctaga tttaaagaat tatttaatgt tttcaaaaaa caaaataaat
    15781 tactaacact gggaagctgt ttggcacagg ttattttcca attatggatt ggaggaatat
    15841 ttcttattga tgttatttta ttagctaaag gaacaataag tattactaat tttgtaatag
    15901 tatttatgta cgctaacatg gtccctactt tatggactac tttaattagt attatttgga
    15961 aatggcaaga ctattcagtg tcattaaata gatattacga atattattca aataacaaca
    16021 aagtggctgg cacaaaaaaa ataaataaaa taaattctat tactggtcaa tcattaaatt
    16081 tttcatataa caaaagaaag gtattaagca atataagttt ttcaactgaa atgaataaac
    16141 tattcatgat atcaggagaa aatggaacag ggaaaacaac actgatgaaa ttatttacta
    16201 aacagttgcc ggttagtaat aatcaattat tcgtcaacgg gatagatatt aatatgattg
    16261 ataacgaatc attaagaagg ttaatttcaa tcgcaacaca aataccatat ataattaata
    16321 gctccgtgaa ggagaatatt ttaatgggga ctaaaaacta tgataaaaaa aaattcagta
    16381 aattaataga actattggga ataagtaaat ttatgacgaa attaggtatt actttggacg
    16441 acaatattta tgatgaaaaa atattattat ctggaggaga aaaacaaaaa ataagtttag
    16501 ctagggcgct tattaaatca gcttcagtta ttatattaga tgaaccattt agaaatttag
    16561 ataatgaaac tcgcgataat ttgagggatt atatactaaa tacaaaaaaa gaagtttgtt
    16621 atataattat aactcatgat cacattctag ataatttttt ggattcaaat gatgaaatat
    16681 tatatttaaa taaggagtat gataataatg aatagcaaaa aacttcagat gggtgaaatt
    16741 gttagaggtc ctagtcaaat atctattgat gttacatata aatgtaattt tagatgtaaa
    16801 cattgttaca atgcgagtgg tggagacaat tgttttatgg ataatgagtt gactgatgat
    16861 gaactgttag aacttacaaa agatatatgt aatttaaagc catataatat ttgtttctgt
    16921 ggaggagaaa ctttactgag aaaaaaaata gttttatctt gtgccacttt attaaaagag
    16981 gcaggtattc ctaatattgc tattgttact aatggatcat tagtaactca ggatgttgca
    17041 aaagaattat tcaaagcagg tattaatcga tttcaagtaa gtttagatgg aaaaaatgct
    17101 agtagccatg aagcacttag attatatcca aacagttttg aaaaggcaat taatgctatc
    17161 aaattattta gagaattgtc cgaagaagtg gatgttgctt tttgtcctac tagttttaat
    17221 tacaatcaat tacgagaagt atatcaacta gtaaatactc ttaaagtgaa gtctttaaga
    17281 attcaaccat ttatgcttag tggtagggga atgcaaaata gggaaatagt gccaactgtt
    17341 tctcaatata gaaaactagt ttctgatatt aactatatta aaagaaatga agattatacc
    17401 aagatagatt ggggagatcc aattgatcat atcatccgac acagaacttt attgaaagat
    17461 ttttcgaatt ttgtttctat taggtcagac ggaggattaa tggcatcacc ttacttgcca
    17521 attcaagttg gaaatattaa atgtcacagt ctttctaaat attggcgagc aggactttat
    17581 aaagtttttt ctttagattt agttcaagat atagctactc aagtttcttc aatacaagat
    17641 atgagtaaag aattaaaaaa aggacatatt aaatggctta atgaagatat agaaattgat
    17701 attctcgatg aatacaaaca tgatatttta atttaggttt atcattggaa agtttagata
    17761 aacaaagtac ttttagaaag gagggaaaaa aatgatttct gaacctcaaa tgaataatat
    17821 gtatgaaagc ccaactataa tgggagctgg tgaagctgcg ccaagagctt gttcagcaat
    17881 tatggtgtgt gtgttattag catctgcagg agtaggtatc caagcaggag cggtgtatac
    17941 ttatgctgta gttgctacag tagctgtagc tgcagccgca gtttggaaaa cagtaggatt
    18001 agttggttag gaattaatag gagactaact gtctcctatt gattccaatg attaagctaa
    18061 ggaggaattt taagaaaata atgaaataca ataatttaaa accgaaatta cttaatgaga
    18121 acatagaata tataagagaa gaaaattatg gatatttaac catagttccc aaaaatggaa
    18181 ctgaatcgaa agataacgtt ttcaatccaa cagtaagata tatattggct aactgtactg
    18241 gaaaaaacag tgtaaaaaaa atagcgaatg gtttaaaaaa gaagtttcct agtttagaat
    18301 atgaaaaatt ctatcaagat gttgtaggaa ctattaaatt tttcaaacac tataatatta
    18361 ttgatatagg agattatagt atggaagaaa agaaaatata taaaattgaa caaaatttag
    18421 aattagaatt actaaatgaa tcacattatg aagaaattta taattttatc acagatcatc
    18481 ctgaaagtat gtattttcat gaagatattt atcatcaagg aaatagtaaa gaattagaag
    18541 ttgtccttag atataattta ttttcgttta cgagtgaatt ttacttttta agacataaag
    18601 agagaattgt aggaatggtt tctataattg ttaatccatt tcaaaactta tcctacagta
    18661 tagtaaatta cctcatatgt gagcctgcat atactacgac tattttgaca acagtattag
    18721 aaaatattaa cgaagtttca attttagaag taaagaagct agtgattcat ttgctaaatt
    18781 caacagaaga tgaatcacta atcccctatt taaaagattc acattttttt aaagaagcgg
    18841 tactatctaa agaatataga gagcatgatg ttttgagata cgcttacatt tattaaataa
    18901 aatttctgat agtattttaa taattcggat catatcataa gatataggag ggaaaagaag
    18961 tgattttatt gattttgtct ctcgtattac tagttgtatc taatctaatg aaaaataatc
    19021 ctcctacaaa aattaattct ttttatggct ttagaactaa aaagtcaatg aaaaatgaaa
    19081 aaagttggca tttagcacaa aaattgttgg gggatttttc aaaaaaaaaa gttatcccag
    19141 tatttttgat gggtattgtt tttttcagta tagagattta tagtttagct attttaaaaa
    19201 atgaaaattt tttcgttagt gtattaatgg gtgaaagttt aatcctaata ctgattttgt
    19261 gtttaattgt tcatgaagtg aataaacaat tgtgaattaa aaaaataaaa acctagtata
    19321 atcactttaa taaattgttg gtagaaggtg atcaaaatgg gagtaaacga aatgatgaaa
    19381 gtcagattga gtttttaaat gataccttcc tcagaacttc aaaacgaatt caaagattcg
    19441 ttgacaaatc ttgagcaaga tcatttaaaa aaatattttc tcttcatttg acgaaaaatg
    19501 tttagaaatc ttatataagt gaagataaag taattggtgt aattaattgt agagaatagc
    19561 ctttgagcaa ttgaagcgat acaattattt agaaaatgaa cttcctccag catactacat
    19621 gattccttac gggacgatgt taaagaagaa agcaagaaat tttagaagta ttagtagata
    19681 aactaatggg cttttctcaa tttaagggag tagcccaaat gtttatcaaa tatggtgcag
    19741 atacagtagc aaccatgttt acagaagaat atcaagtaga ctattatgaa gtaatagata
    19801 aatgtgtaca ttttgtttat gaaaaagtgt ggaattttat gaattttgcg ataagtgaag
    19861 aaatcttacg agtacaagaa aacagaacag gaatatttac cagagacaga aagcttaatt
    19921 tgattgcgca ttatatacgg aatcgctggt catcaatacc aaattttaat tataaaaaat
    19981 aattatgaac aatcttttat acaatataca caataaatca agtagtattt atgaaaaaag
    20041 acttcaaaaa cagaatgatt ttctatcagt cttagaaatt atagagctaa aaattttggt
    20101 cacgcgtaga aacgcgtttt ttttgttttt ttgatagtaa agaacgtaca aaacaatgag
    20161 aatttaaaag attgtactac cagttttttt accttttcat ttgagtttag agtaagtaaa
    20221 atattactat aaaaacacat attcttgaag aaatcttttt tttgctgtat tcatttagat
    20281 tatgatagag aaaatacgtg agagggggac ctgggatgtg gctatctgat gaaaaaattc
    20341 agtggcgtag ctatcagaag cgtatattga ctaactttgc tacttattta aataacaatt
    20401 cagtccatct agtggctcca ccaggggctg gcaagacctt tttaggaatt gagatgatcc
    20461 gacgtttgga ttgtaaaacc ttaattttgg ttccttccct aatattgaag aaacagtggt
    20521 taacaacaat cgaggaatat ttcttagtaa atccagcaga tagttgctat ctttctgggg
    20581 cgattgaaca tcctagtcat attacagtgg aaacgtatca gaccatttac aaccgactga
    20641 aggaggagcc aacttatggt gatcagctgg agctgttagt tttttgatga agctcatcat
    20701 ttaaaaaaaa gctggggtga attgttgatt gacttgaaga aaaaacaacc gaatctgttg
    20761 acgatttcct tgacagccac gcctccgatt gatagctctc agacggagtg gaagcaatat
    20821 attgagttaa aatggcctga ttgacgaaga aattagcatt cccgagttgg taaaggagga
    20881 ggttctatgt cctcatcagg attttctata tttgattccg gctaatcagg aaactactca
    20941 gcattatttt acctttttag agaaacaaaa tgagctggtg gaagcgttaa tggaaagcca
    21001 agagacggtg gattatttgc tttctttaga atttgttcag catccgcttg agcattcgaa
    21061 ggaaatttat gaaaattttg agctgttcac tagtgcatta atttacttga atcatcataa
    21121 ctatgaattg atacccgaac attggcagct attaggcagt tctaagcagc aaggtaaaaa
    21181 agcttcagtt ccatctttag gtaaaaaaga gctgatcact atttatcagc agatgtttga
    21241 agatcaaccg gaattgccta ttttttatca attgaaaaaa gctggatgat tgtttgatga
    21301 gtcgctgcat ttgtttccag agtatccaga cactgttggt agtcaggcga ttcctgagaa
    21361 aaagcaagca gtttgtcaaa ttttgttaaa agaagaagtt cagctgaagc aagaactgac
    21421 agcggtcatt ttgatggatc gtatttgcaa ggaggtatta gcaggcagtg atggtacctt
    21481 agcttatggc gtggttcctt tgtttgatga agttgttcgt ctattaggta acactcagca
    21541 aatcggtgcc atctgtggag agttcttact gttaccgaaa atgctagtaa ctgttgagct
    21601 ggcagaacta gcgacgggta tgaatgaaca tccttatatg tcgggttatt tttatttgac
    21661 ggtggatgac aaaaaccgcc aagcattgat gcaagcagtt actcgcctat taaccgaaaa
    21721 gaagttgaat attttggtag gaaccgttag tttgctagga gaaggctggg attgtcctgc
    21781 aattaatctg ttgatcttgg ctaatcaagc ttccactttt gtgcaaacac aacaactacg
    21841 agggcggggc ttacgcctag gtgcacaaaa taaagtaacg aacatttggc atttagcgat
    21901 tggattaccg ggagttcctc tgtctgaaca gcctgatttg aaaagaatgc tacggcgttt
    21961 gggcaatatt tcaggtctga cctttgaatc ttcaccaaaa attgagagtg gtggcgatcg
    22021 ctttgcttta ccactggaat atacgaccac cacgatggaa acttatactc agtcgatgtt
    22081 ctatttttct tgtgaacgaa gtcggttgaa agaacaatgg agtactgctt tagctgatgg
    22141 ctggcaccta tctatgccaa tcgtcgttag gaaagtcgat caggtaaagc aaaaacaaga
    22201 aacttcattg ccgatagtag taaaaaatat agagcctcta agcttctggt cagctatatt
    22261 taaaggtaac ttgctggaat atttccaaat tcgtaggcga caaaagctat gggaaaagga
    22321 atgtgcccgc aaaaaaagct tggccatatg cgttcaaacg acgttgcagc gccttggtga
    22381 agttagtgat agaaaagtaa tcgtagcggc tgatcagcag gaatttagat gctatcttga
    22441 gaatgctggc tatcgagaag gacagctttt tactgaagct atgagggagc ttttagcacc
    22501 gctggagaat ccgcggtatt taatcgaagg gaaaaagata gttctgacgg taccagaacg
    22561 ctttggccga aataagcaaa tcgctaacct ttttctaaac gaattaaatc aatcgtcgct
    22621 gcccaaatgt cagttggtgt atactagaaa tcctgaagga cgccaaaagc tagtgactta
    22681 tcgaatgagg caagtcttcc agacccgcga tgaagtaatt attcaaaaaa acatttggac
    22741 gtaagttgat gaaaataaat tttgctagag tagaacaagt ccgagaatta agttgctact
    22801 taactgataa tgccaaggac aggagaatct tttcttgacg actgaaagaa actatttgac
    22861 ttatataacg gtgtgtgatg aatatttgca aggcattctc ttaaaaagta ctcgtaatag
    22921 tttttttgaa aacaaaacag tactttatta ataggactga cagagcgata taagaacatc
    22981 tgcagacaga ggtgaatgct tttctcatga tcgatgaaac aatggtactt cggatattct
    23041 tgcttttagt ttgaaccgtt gtgattttct ttgattccta aaagtagttt tatagaatga
    23101 aaatcaagca tacggaatgc tcctttgatt gtgttgtggt gattcaatct aatggagctt
    23161 ctatatgttt ttttatttgc aaaaagaacc gatagaatat ttttatcagt cttagaaatt
    23221 atagagccaa gaaatttcaa gcacacataa aaacgcgtgt tttatgcatt ttatgagcaa
    23281 aaataactaa agaccattac gtaaaacata ggtaaatcag cattcaatct caaattatgt
    23341 gatcaaatgt tgatattatt tgattataag ttttcttttg ctattatcag tctgcaaata
    23401 aacagattat agagtatgaa tgtagatagt tcaatctttg tttacgacct cacatgtttt
    23461 tttactcaag agaaagtaat ataaagtgac tatatttgac agaactttac ataaaaaaag
    23521 tagttagttt tctgtagtaa tcagaaatgt aaataaatta aaaaaaagaa atttagcagt
    23581 tatatatttt taaaatattt tgggtgagta gtcaatacaa cattttgttc atcaagcatt
    23641 aaatgaagat gcaaggatgt taagaagtta gcttcttcag ggtcttgcat ggtcaaggct
    23701 ttaaatagtt gatgatgttg ttccataatt ttttcccatt ttaatccttc cgtttttact
    23761 cgtaaccaac gaaatcgttc caagtgcgta ttattatctt ccaaccattg ccatacttct
    23821 tcccgttctg caatttcaaa acaagcttta tgaaataaat tatcattatg aaaaaaacta
    23881 cgagcattct tttgactaac agctttttct tgttctgcaa ttatagtttc taaaacttgt
    23941 ttcttttgat tattcatctt ggcacaacac tctagcataa tttgtcgttc taaatgttct
    24001 ctgacaaaac gagcattttt tgctaaatct aagtcgatta atgagatata atttccactt
    24061 tgaggaattg tatcaacaag tccttgatga cggagttgaa tcaatgcttc acgtattgga
    24121 gtacgaccga tagaaagcgt ttcttccaat tttttttctg atactttttt tccaggttct
    24181 aagtcagcgt agataatttg ctgacgaata gattgataag cttgattttg gagactagtt
    24241 ttaattgtca tatttaatcc ctctcaaaat ttcatttttg aatttgatat tttagttcta
    24301 tgctcgttat ataatatcaa atttcaaagt aacatactag ctattaaaaa aacaaaaaat
    24361 actcaaataa gatcaaagag aaattaagct ttaatttaag ttagttctaa aaaatatata
    24421 ttggtgtgct tttactaaaa aaagacacaa taattctgac atggtagaaa aaagaatatt
    24481 tttagttttt tcttattgac agcgattaca ttcgatttta tactacaatt gtaacggaaa
    24541 ctaacatatc agtatggagg ggtaaatatg gttttgatta aagactggca agattctatc
    24601 gaaggattga acaaaatgca agttcaacta ccgtatttta atgtcgaagc ggtcaaacaa
    24661 gaaggtcaga aacatccagt gtggcttcat tttggtggag gaaatcttta tcgaggattt
    24721 catgcagaaa ttgctcaatg cttagcagat caaaatgaat tagaatccgg agtagttgtg
    24781 tgtgagacat ttgatgaaga agtagtagaa aaagcttatc atgactacaa caatgatatt
    24841 ttggagattg ttatgggtga agatggtgaa ctcaaaaaaa gacttttagc agtaacagct
    24901 gatagtttat attgcaatcc aaagaatata acggactatc aaagagtggt taaatatttt
    24961 gaagatccta cattacagtt tacaacgttt actattactg aaaaaggata cagtttaaaa
    25021 gattctaaag gacaattttt ttcggtagtg gaagcagata taaaatctgg acctaccaat
    25081 cctcaacata cgatgagtat tgtgacatcc ttgttgtata gcaggtttaa tgctggagag
    25141 ttaccgattg cgatggtttc tactgataat ttctcacaga atggtcaacg ttttcaagat
    25201 agcattttta tgattgcaga agcttggcaa aaaaaaggat acgtttctga aaaatttatt
    25261 gcttatttaa aggataaaga aaaagtgact tttccttgga gtatgattga tagaattacg
    25321 ccaaatcctt cggaaagtgt caaaatggat ttattgaagg cgggtttcga agacttagat
    25381 ataattcata ctgcgaaaaa taccaatatc gctgctttta ccaatactga aaaagtccat
    25441 tatctagtca ttgaagatag ttttccaaat ggtcgaccta aactggaagc ggctggtgtg
    25501 attatgacag atagagaaac agttgataaa gcagatatca tgaaggttac gacatgttta
    25561 aatccgctcc atacggcatt agcaataaca ggttgtttgt tggggtataa atcaattcag
    25621 gcagaaatgc aagatgatga tttggttggc ttaattaaag ggataggtta tgtagaaggc
    25681 ttaccagtag ttgaaagtcc aggcgttatt gatccaaaac aatttattga tgaagtaatc
    25741 aataaacgtt tgcctaatcc ctatattcca gatacaccgc aacgaattgc aacagatact
    25801 tctcaaaaag tagcgattcg ctatggtgaa acaattaaaa agtatgtcga aaaagacgga
    25861 aatgcagaaa atctaacatt tattcctatc gctattgctg cttggttacg ttatttattg
    25921 gcagtcgatg atcaaggaaa aaattttgtg ccaagtcctg atcctttatt ggtagagttg
    25981 caggaaaaat tctcagtatt gaaattaggt gaacaagatc ctgcaaagat tcattgtgcg
    26041 gtacaggaaa tcttagaaaa taaggaaata tttggtttag atttatatca agttgagata
    26101 ggaaagaaaa ttgaaaaaat cttttgcgaa atgctagtag gtcctcaagc agttcgtaaa
    26161 acaatcaata aatatataac agaagttgga ggaaagtaac tatggaaatg acttttagat
    26221 ggtatggggc aaatgaagaa aaaatctcac tacaagaaat tcgtcagatc ccagcaatga
    26281 aaggaattgt tggaacatta atggatatac cagccggaga ggtctggccg agagaacgaa
    26341 ttaaagcttt aaaagatgaa gtcgaagcca atggccttac cttgaaagtt attgagagtg
    26401 ttaatgttca tgatgatatc aaaattggtt tgccaacacg agatcaatac atcgagaatt
    26461 ataaacagac gattcgcaat ttggcagaat ttgggattga ggttatctgt tataatttta
    26521 tgccagtctt tgattgggta aaatcagatt tggattatca tttagatgat gggtcttcta
    26581 cgttagccta tatttcaaaa aatattccta ctgatccaga ggaaattatt agaaaagtag
    26641 aaaattctac taataatttt gaattgccgg gttgggaacc tcatcggtta tcacaaatca
    26701 agcaattatt agaagcctat aaagaagttg atgaagcgaa attgcgagag aactttgcat
    26761 attttctcaa agcaattatc ccgacatgtg aagaagttgg gattaaaatg gcagttcatc
    26821 cagatgatcc accatattct attttcggat tacctcgtat tgttaaaaat cgtgacgatt
    26881 tagaatggtt gtgtaatgta gtggattctc cttctaatgg tattacgtta tgtactggaa
    26941 gtattgcaga acgaccagat aatgatgtct atgaaattct atctgaattt tgtcaacggg
    27001 atcggattcc ttttgcacat gttcgtaata tcaaatattt aagtgataat ggactcgatt
    27061 tttatgaagc gccgcataaa tcagaatatg gttctctaga tatgtataag atattaaaaa
    27121 caatgtacga taatggattt aatggctata ttcgtccaga tcatggtcgg atgatttggg
    27181 gagaaaccgg tagacctggt tatgggttat atgaccgagc tctaggtgca gtttatttaa
    27241 atggtatttg ggaagcgctt gaaaaggggg cgagtaaata attatataat ggagtctatt
    27301 tagaagaaaa attttctctt ctaaaaacta agggaaggaa aggagtgctt aaatatgttt
    27361 ttagataaga actttttatt agagaatgaa atagctaaaa gactttttca taaatttgca
    27421 gaacaccaac caatcatcga ttatcattgt catttagacc cagcatgttt gctagaaaat
    27481 aagcaataca agaatttgac tcaggtatgg ttaaatgata aaggagctgg agatcattat
    27541 aaatggcgat tgatgcgagc taatgggata gaagaagagt tgatcagtgg tgcaggtaat
    27601 gattatgaaa aatttttagc ttttgtaaaa acagtggaga aggcgccagg taatcctatt
    27661 tttgaatggt ctcatttgga attacaacgt tactttgata ttgatttgac aatttctgag
    27721 aagaatgcac cagaaatttg gcgacgtgcc aacgaaaaaa tccaaacgga ttcctatcgc
    27781 ccaaaagaat tgctgaagat gatggatgtg aaagccattt gtacaacgga tgatcctaca
    27841 gatgacttgc gatatcatac tgagctttct aaacaggata attctttcaa ggtattacct
    27901 actttccgtc cagataaggt ctggagtatc aaatcatcag gattttcgaa ctatatttta
    27961 aaacttagtc aagcagcggg tataccaatc aaaaacttta atgggttgaa gcaggcattg
    28021 agccaacgag tggattattt tcatcaaatt ggctgtcgat tagcagatca cggtttgaat
    28081 acttattttt atgaaatagc agatgaaaaa atactcgaca atctgttaat aaaagcttta
    28141 gcaggaaaaa cggatttcca tttatcagaa attgcacaat ttgttacagg tattcaatta
    28201 catttgatgt ctgagtatac aagatatggt tggacgatgc aaatgcattt aaatgcttta
    28261 agaaataatt caagtatatt aaaggaacaa attggagtag atgtcggtgg tgattcgatg
    28321 ggggatcaag ctagtttgac acaaaatgtt gtagcattgt tcgacactgc tgaaaagagt
    28381 gggtgcttac caaaaacaat tttatattca ttaaatccaa ccgattggct tccactagca
    28441 acagcaatgc aaagcttcca agggggtatg attcaaaaaa tccaattggg atgtgcttgg
    28501 tggttcaatg atacagctga aggaatgcgt caacagttga ctacgatggc gcaacaaagt
    28561 ttgttgtcga attttacagg tatgttgact gattctcgaa gttttctaag ctatcctcgc
    28621 catgagtatt tccgtcgagt attatgtcaa ttactaagcg agtgggttaa agcaggtcga
    28681 attccagatg atgaaatgta tcttagtcag atagtggaga atatttgtta tcgtaatgcg
    28741 tataattatt ttaatttttt tgaaatcgaa agtagaaaat aatacaaaaa ataaaagctt
    28801 aaatttattt attgtaagtt agtacaacta gctagagtat gtatccataa aagaaggaga
    28861 tagatatgaa aaaagtagaa attttaagta agttagaagc ggctggagtc attgcggttg
    28921 tacgtggaaa aaataaagat gaggccatta aagcaataaa tgcaattact gcgggtggaa
    28981 ttaaagggat tgaactaact tttacagttc cgaatgctga agaaataatc aaagaaattg
    29041 ctaaaaaata tgctgatcaa aaagatgtaa taattggagc cggtacagtt ttagatgcgt
    29101 ttactgctcg tattgcaatt atggctggag cagaatatat cgtaagtcca tcttttgatc
    29161 gacaaacagc agagatttgt aatttatatc aaattcctta tttaccaggt tgtatgacaa
    29221 ttactgagat aaaggaggca cttaccgctg gagtagaaat cattaaacta tttccaggtt
    29281 ctgcttttgg tccatctgtt gtaaaaacgt atcaagctcc attaccatat gtaaatataa
    29341 tgcctactgg cggagtaagt ctagataact tagaagagtg gtttgaagca ggcgttgttg
    29401 cagttggtgt aggtgggaat ttgttggctc ctgttgaaaa aggtgatttt gaagaagtaa
    29461 ctcgaatagc tagtaaatat atagataagt tttatgagat aaagaattat taggagaaaa
    29521 aataaataat actataaaat gtaaaaaata aatactaact ctttatactt cagagtatag
    29581 tacgtaagtt ggcagttcaa cagaatgaaa acgcttaaca ataggaagga gcgcaaatta
    29641 tatggaaaga aaatttggtc taagagacaa aataggatat atgtttggag attttgggaa
    29701 tgatttcact ttcatttttg ctagttcttt tttaatgatt ttttatacaa aagtattggg
    29761 gataagcgga gctaccgtag gaacattatt tttagtagca agatttattg atgcagttac
    29821 agacataaca atggggcgta ttgtggatgg atcaaaacca tctaaaactg gcaaatttaa
    29881 accgtggatt ttacggatga gtggtccagt cgctttagca agttttttaa tgtatcaaac
    29941 atcaatggca tcagcttcta tgagtattaa agttatatat atgtatgtaa catatatact
    30001 atggggaagt attttttata catctattaa tattccttac ggttcaatgg catcagctat
    30061 cacttctgtt gcagaagaaa ggacagcgtt atcaacattt cgtagtatag gagctacctt
    30121 agcaggtatg ttcattggcg tgttaacacc aatttttatt tatacaagag atagttctgg
    30181 agctcaaatt gtacgtggtg gatcaacttt tacaattgtg gcaggagtat tttcagtgtg
    30241 tgcagttatt tgttacgtaa tatgttataa attaactgta gaacgggtta aaagtgatga
    30301 atcgcagtta aaagtttcat tgttgaaatc tttaaaagga ttagctaaaa atagagcgtt
    30361 gctttctata atagctgcag ctattttttt gttgcttgct cagcttttaa caatgagtat
    30421 gaacaattat gtatttccag attattataa taatgcacag ggaatagttt taatgaactt
    30481 tatcaatcct attttgatgt tggtacttgt ttcacctgta agtttaattt tcagtaaaaa
    30541 atacggtaaa aaagaattgg cgtcagtagg gatgttcttc tctgctattg tatatttact
    30601 tttatttttg ataaagccta gtaatatgta tgtattctta gttcttactt ctgttggata
    30661 tatggggtta ggtattttta atacagtcat ttgggcgaat attactgatg taatcgatga
    30721 tcaagaagtg aaatctggcc aaagagaaga tggaactatt tatgcagtat attcttttgc
    30781 ccgaaaaatt ggacaagctt tagctggcgg tgtcggaggt tggactcttt ctataatagg
    30841 gtatgattcg ttagcaaaag tacaaagtgg agaagtttta caaaagctgt ataactcttc
    30901 aacgttgatt ccagcagttt gtttctttgt tgtaggattg attttatttt tcgtatatcc
    30961 attatctaaa gaaaaagtat tagaaaatta taatttgcta aatcaaaaac ataactagga
    31021 gaaaaaacat gaacagttgg atattttgta ttgattcaga tggttgtgtc atggatacaa
    31081 tgacttataa acacaatctc tttttcgggc caattgctgc agagattttt gatgttcatc
    31141 atagggaaaa attcctacag gattggaata atattaattt atattcagag actcgtggaa
    31201 taaacagatt tttaggttta gttaaaacat tagaaaaaaa tgatgtacca tcgattaatc
    31261 gattaaagca ctgggtatct gtaacaaaag aattgtctaa tccatctttg aagagggaaa
    31321 tagaatactc tcagtcagag gatttattaa aagctttaaa atggtcggaa gaagtcaatc
    31381 gacagattag tcaagctaaa ggattagata aacccttttt aagtgctaga gatactataa
    31441 aagctctgaa aaaatatggg aagataatta ttgtaagctc agctaataag gaagctattc
    31501 aagaagaatg ggaaagacat gaattattat ccttagttga tgaactgtgt tgtcaagata
    31561 aaggaaaaaa agaagacatt attcgaagtg taattgaaaa tggttgtgat ttggataaga
    31621 tattaatgat tggtgattct cctggtgatc tagaagctgc aaataagaat aaagttttct
    31681 tttatcctat tttggttaat aaagaaaaag aatcatggga aaacttaaga actgatattt
    31741 tatttaaatg tttatcggga gagtatatag atattgaaca aaaatatata gatacttttt
    31801 ggaaaaatct tactaataag tagaggagaa agaaaatgac tataaaagta gacttacgaa
    31861 aaaaaccata tcatttagat gaagatgcta tttcttggat tgaagagacg attgatgcta
    31921 tgactttaga agaaaaaata ggtcaattat ttgttaatat gggatctagt cgtgataaaa
    31981 attacttaag tagtatggtc aatcagtacc atattggagc tgtacgatat aatccaggaa
    32041 ctgctgatga ggtatatgat caaaactata tcctacaaac agaaagcaaa attcctttat
    32101 taattgctgc taatacagaa gcaggtggta acggagcttg tacagatggt accgaagttg
    32161 gtgtagaagt aaaaattggt gcaacaaatg atgcgaaata tgcttatgaa ttagggagag
    32221 tttctggaat tgaagcatcg gctattggat gtaactggag ttttgctcct attgtagata
    32281 ttaatagaaa ttggagaaat cctatcattt ctagtcgatc ttttggacaa agccctgata
    32341 aagttttaga acttagttta gcttatatga aaggtattca agagagtgga attttgcctg
    32401 cagctaaaca tttccctggt gacggtattg acgaacgaga tcaacatcta tcttttagta
    32461 ttaatagttt aagtacagaa gaatgggata attcttttgg aaaagtatac tcaggcttaa
    32521 tagaagctgg attaccttca attatggcag gtcatatcca tatgccagct tattcaaaac
    32581 attttaatct tgatctgaaa gatgaagatt tacttcctgc aactttatca aaagaattaa
    32641 tcactgactt acttagagga aaactcgggt ttaatggatt agtagttact gatgctagtc
    32701 atatgttagg aatgactggg gcaatgaaac ggtcagaatt attgcccact tctattgcgg
    32761 ctggatgtga tctattccta ttttttaatg atccagatga agactttaat tatatgatgg
    32821 aaggttatta caaaggaatt atttcagaag agcgtttacg tgatgcatta agaagaattt
    32881 taggcttaaa ggctaaacta ggtttgcata aaaaagaaaa gaataaaatt ttgttaccga
    32941 aacaagaagc tatggaaaaa attggtttaa ttgaaaataa atcaattttt aaagaggtag
    33001 ctgatcaagc aattacacta gttaaagata aacaagatat ttggcctatt gtaccagaaa
    33061 aatataaaag gattttactg gtagatgtac atggggtaaa aggtgggttt ggtgaagtag
    33121 ttgctggaaa tagacctcag gccattgatt tattgaaaca gattttgtca gcaaaaggat
    33181 ttgaagttga aaaatacatt tcacctgaag aagaattgaa aggattatcg caagaagaaa
    33241 aaattaaaaa tatagctgga atatatgctg ctaagagacc aatcaaagat ttaactgaaa
    33301 aatatgactt gattattaat atagctaatg ttaacccagg tacagttcaa agaattgttt
    33361 ggccagcaag taaaggtact ccggatattc cattttatat tcatgaaatt ccaacaattt
    33421 ttgtatctgt acagtgtccg tttcatttag ctgatgtgcc tcaagtaaaa acttatatca
    33481 atacttatga tggaaaacaa gaaactttag aaatattagt ggacaaacta atgggccttt
    33541 ctgaatttaa gggaataagt ccaatagatg cttattgtgg gttaaaggat acatttattt
    33601 aatatttaaa ttatatagga gtgatttaca tgttatatcc cattatgaca gagacgaggc
    33661 aagtaattga tttaagcggt ctttggagat ttaagttaga tacaaatgat gacttacaag
    33721 aagaacttgc tttagaacca atggttgaaa aagagagtta tccaatttca gttccttctt
    33781 cttataatga tttatttgaa acagagaaca ttagaaatca tgttggatgg gtgtggtatg
    33841 aaaaagaagt gattcttcct aaaaaattat tagaagaaag attagttctt cgttttggat
    33901 ctgcaacaca tgaagcaaaa gtatttttga atggaaaatt gattaatcaa cacaaaggag
    33961 ggtttacgcc atttgaatca gaaattaatc agtttataaa agatggaagt aacagaatta
    34021 cagtggctgt aaacaatgtt attgacgata gcactttacc tgtagggatt ttgaaagaaa
    34081 cgatacagga agatggagac ataaaaaaag aaaatttggt taattttgac tttttcaatt
    34141 atgcaggcat taatagaccc gttaaattgt atacaactcc gaaagactat ataaaggatg
    34201 tcactattaa tacaaaaata attgattcag atgcaaatgt agaatttaat atcgatacag
    34261 aatggaccgg agaaattcta atagcaatct atgacgaaga agctcaactt atagcagaag
    34321 ctaaggggaa agaaggagtg attcaactta agaacccaac actttgggag cctctaaatg
    34381 cttacttata tagtatgaaa gttagtttaa ttaacgaaaa taaagtgatt gatacgtaca
    34441 cagaagaatt tggcattcgt agtattcagg tttcgaatgg gaagtttttg attaataata
    34501 aaccttttta ctttaaggga tttgggaagc atgaggacac gtatattaat ggtcgtggtt
    34561 tgaatgaagc agtaaatatc aaagatttta atttaatgaa atggatcggt gctaattcat
    34621 ttaggacttc acattatcct tattcagaag aaattatgcg cttagcagat cgagaaggta
    34681 ttgtagttat tgatgaagta cctgcagtag gattacattt gaatttcatg gctactttat
    34741 tagatgacaa cgtagaaaaa catgatactt ggaaagaaat acggacagaa aatgctcaca
    34801 aaatggcgat taaggaattg atagaacgcg acaagaacca tccgagtgtc gtaatgtggt
    34861 ctattgcaaa tgaaccagat tcggattctg aaggcgctaa agaatatttt gaacctttag
    34921 ttgaattgac taaagaactt gaccctcaga agagacctgt aacaatcgtt acttacttaa
    34981 agtcaacacc agatatttgt aaagtaggag atattgtcga tgttttatgt ttaaatcgct
    35041 attatggttg gtatgtttca ggtggagacc taaaacatgc aaaatatctg ttgaaaaaag
    35101 agctagatgg atggatggaa agatgtcccg atacgccaat aatgtttaca gaatatggag
    35161 cagatacagt agctggactt catgatacag taccaacaat gtttacagaa gagtatcaag
    35221 tgggttatta cgaagcaaat cacgaagtaa tagataaatg tgacaatttt gttggtgaac
    35281 aagtgtggaa ttttgcagat tttgaaacaa gtcaagggat tttaagagtg caaggaaata
    35341 agaaaggaat ctttactaga gatagaaagc ctaaaatgat tgctcactat ctaaagaaac
    35401 gatggtcatc aataccaaat ttcagttata aaaaataatt taccttttat aaggaatata
    35461 aaaaaggagt ggctgcttaa ttgccaactc ctttttttta taaaaagatt ttctgtttaa
    35521 agaaggatat ttttttctag ttttttcgaa aaaaaggcgc tttctgattg ttgtattagt
    35581 ggaaagggga aaaaaggagc atatttatca tgcaacaaaa ttacaatgag aaatctatac
    35641 aacatcaatt tgatgcctac tgtaaaaaag tattacgaaa cgaaactaag tctattcgaa
    35701 aacgaaacat taacataaca gataatgagg aaccattgac gaattcaaat gaaggtaagt
    35761 actcccaaac tcattttgat gaacaagatg tctatttctt atttggaatg gaaatattaa
    35821 tttcagatca aaagttatcg gcagccattg atcatttatc ggaaataaga agaaaggtta
    35881 tcctacttta ttattttgca ggttttaatg atactgaaat cggtaaaatc ctcaatatga
    35941 gtacaagtgg tatctggtat caacgaaaga aggcagttga acaattaaaa atggggtatg
    36001 acttatggta gacaacccca aaaaacggca gtatccatta gttcctctat ccattatcca
    36061 atctgctcta gaagaagatc caatttcaat caagatgata ctcaaacatt atcatagtta
    36121 tatagctaag ttatgtttga caaatggatt taatgaggcg gggcaattta ttacctatgt
    36181 agatgagtat atgctaagac agctagaaat taaattgata gaagcgatct taaaatttaa
    36241 aattaactaa ctaaaaagtc ccctttccac ttattctgtt taaaagaatc ttctaatagt
    36301 taaaggatta gtagattctt ttaaacagaa aacctattga tcattgaaat ttaaatacat
    36361 tctatcagta cgttaacact tcaaggagca tattttagtt gcgccatgat ttcttactgt
    36421 aaagaaggag cgataaacat tctaaaataa tttttatagt caataaaaag cgatgatttg
    36481 aagttgggat aatgatactt ccgtaaggct cgccgtaaac ttggagagag gtgaaattcc
    36541 tatgtgcttt tgacaaaagc agctgattaa gaattatttc catatgttga tttcaacatt
    36601 ttttctatta tttttaagac caaattcagt gaggcaaata gcatcatgta aatttagttt
    36661 ttctattgaa cgttctccac tttttagttt ttttatagta ctcaaattta atcctgtatg
    36721 tttagagatc tcatttgcag tatggttttg taaagtttct cggattattg ttatattagc
    36781 aatttcatta cgcataatta tgttcctttc ttatcaaaaa tatcaatgac tacagtgggc
    36841 ttttagtgaa aaaaaggggt actacagtct cttttgaaaa caaaaaaata cagtatatac
    36901 gttttctaaa tgctaagcac caacgtttac actgtattta agggtttggc acccaatggg
    36961 ctgtgagggg ctcgaacccg cgacccgatg attaagagtc atctgctcta ccaactgagc
    37021 taacagccca aattaatact ttgttattct aacacctaaa ataaaaaagt gcaatactta
    37081 ttatcagaat tgagagtgaa ttttatggat tttcaagatt atgaaaaaat atttgaaagt
    37141 tatccatcga tattgaaaat tactgattta actaaaatgt taaatattgg aaaaaataaa
    37201 gcttataatt tgctgaaaga tagatcaata gaaaatataa aaattggagc aaggtacaaa
    37261 atacctaaaa aagctgtcat tgagtatata ttgaaaatac aagatgaagg taaaaatcat
    37321 gctataataa aatagtcaaa atttttgccg atatttcagt ttaagaggag agattgaatt
    37381 gaaattaaga gcaaacataa ttgaaaaaag aggaacatta caagtagttg tatcttataa
    37441 agactctaac ggaaaaatta gacaaaagtg gaaagataca aaattaaaaa agaaaggaaa
    37501 taaaaaacga gcagaaaaaa ttagagatga atttttacta gaattggaaa gtcaattagc
    37561 tatggaatgt gaatgtgcta atccaaattt attgttttat gactatttgc ttaattattt
    37621 ggaagtatca aaaaaacaag tagcatttaa cacatatgtt gggtataagc attacgtcca
    37681 taatcgaatt tacaagtttt tttatcctaa aaaaattaaa ctaaataggt taaagcctta
    37741 tcatttacaa gagttttatc aatatatgtt aaatgataac tgtacagcaa atacggttat
    37801 tcattatcac gcatttatac gcaaagctct tcaagaggct gttattacag aattgataag
    37861 tacaaatgta gcagataaag taagaaagcc aaaaaagaaa caatttgttt ctcaagttta
    37921 taatcaacaa gagatcttga aattacttga cattttgtca gaagaaaaac tctatttggt
    37981 agttctttta acagcatttt atggattgcg aagaagtgaa gttttaggat taaaatggtc
    38041 tgctattgat tttttaaaca aaaaaatcat gataaaccat gtaatcatag aaaatccaga
    38101 gaatatgtca cagttaataa agaaagatca aacaaagaac tcttccagct atcgtacatt
    38161 acctttggtt gaaacgattg aacgagcatt agtaaatcaa gctaaatggc aacaagataa
    38221 tcgtattctt ttaggagatg attatatttt agaagataat gagtacgtat ttactatgga
    38281 agatggacgg ttgatgaagc ctcaatatgt aacgcatcgt ttgagtaagt tgattaagaa
    38341 aaatggattg aaaaaaattc gctttcatga cttgcgtcat tctaatgctt caataatgtt
    38401 agatagtggc cagaatatga aatccattca agaatggctg ggacatgcat cgtattcgac
    38461 aactgctaat ttatacacgc atttaaccgc aggagtaaaa gatcgtgcag cagaagcatt
    38521 agaaaacgca tttgggttta aagaataaaa aaacaaaaaa caatatcttt ggggtatcgc
    38581 ttcttgtttt tagaattttt ttgttggcaa tttgttggca gtatgttggc aatgaaaaaa
    38641 ttcacaaact aaaaacctat ttataaagct ttataaaact tttttcacaa atagagcatt
    38701 tggttaagag tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata
    38761 atagtagcac taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt
    38821 tacttcctta taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat
    38881 accaacaaac aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc
    38941 tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag
    39001 ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta
    39061 gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat
    39121 gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct
    39181 gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt
    39241 tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat
    39301 ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa
    39361 agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga
    39421 aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct
    39481 attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc
    39541 tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac
    39601 tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt
    39661 aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg
    39721 atgttccaga agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt
    39781 ataaagatca ggtatataca gataaaattg acattacccc tgaagagatc tatcaacgtt
    39841 tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg
    39901 agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga
    39961 gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg
    40021 tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat
    40081 ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa
    40141 gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg
    40201 gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg
    40261 aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa
    40321 cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc
    40381 atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac
    40441 aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg
    40501 gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag
    40561 gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg
    40621 cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata
    40681 cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt
    40741 tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag
    40801 gctatgacgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag
    40861 aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc
    40921 gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa
    40981 ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca
    41041 accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg
    41101 aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag
    41161 cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac
    41221 tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg
    41281 tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc
    41341 gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg
    41401 gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt
    41461 cgatcagtca attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa
    41521 attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa
    41581 gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa
    41641 gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg
    41701 actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc
    41761 gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc
    41821 acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa
    41881 ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc
    41941 gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt
    42001 gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc
    42061 ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa
    42121 gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt
    42181 attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc
    42241 catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat
    42301 atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca
    42361 cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg
    42421 gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt
    42481 tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg
    42541 acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa
    42601 cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg
    42661 aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg
    42721 attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt
    42781 caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg
    42841 atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt
    42901 tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg
    42961 atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac
    43021 gtgattttaa gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg
    43081 ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt
    43141 ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc
    43201 aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa
    43261 tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt
    43321 taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg
    43381 tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct
    43441 ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa
    43501 atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag
    43561 tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt
    43621 tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag
    43681 atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc
    43741 aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata
    43801 tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag
    43861 aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac
    43921 tgaagaagta tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt
    43981 tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg
    44041 ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc
    44101 cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag
    44161 gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc gattatcgga
    44221 tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag
    44281 ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc
    44341 aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc
    44401 agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat
    44461 ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat
    44521 ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga
    44581 attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg
    44641 tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta
    44701 atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga
    44761 tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt
    44821 cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag
    44881 acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc
    44941 cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg
    45001 gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc
    45061 aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt
    45121 ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg catttatctg
    45181 atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat
    45241 tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt
    45301 cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg
    45361 aaacaagcga taaagtggtc caaattgaga gcgtcaacgc tttcagcaat acgatcgtga
    45421 caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt
    45481 tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca
    45541 gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc
    45601 ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc
    45661 ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg
    45721 gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg
    45781 aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg
    45841 ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag
    45901 cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca
    45961 ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg
    46021 aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc
    46081 gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg
    46141 tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga
    46201 aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac
    46261 atgattttga aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa
    46321 cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc
    46381 ttctattttt atttttttag catattaaaa aaataaccgt attatcgact gactacactg
    46441 atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct
    46501 gccatgctga tcgtactgtc gctagcagcg tttctttgta aataatcatc acttaacaaa
    46561 taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa
    46621 tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga
    46681 tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag
    46741 aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca
    46801 atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca
    46861 tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt
    46921 ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa
    46981 tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt
    47041 gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat
    47101 tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca
    47161 ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa
    47221 atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga
    47281 cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg
    47341 ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca
    47401 ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg
    47461 atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc
    47521 ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat
    47581 actgtctgga caggtgctga agggatacta ggagcagtta caactgggct catcatcgga
    47641 tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct
    47701 gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg
    47761 cttatcggtt cagcggtttt tcaacatttg ttcagaatga ctgttgttca aggagtatgg
    47821 acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta
    47881 ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg
    47941 ttggttagtc ccgtcttgca agcgaattca ttagcaaatg cagaaatctt agcctcagga
    48001 caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc
    48061 atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg
    48121 aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc
    48181 aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt
    48241 attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt
    48301 cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta
    48361 gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt
    48421 tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa
    48481 aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag
    48541 attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc
    48601 tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat
    48661 cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa
    48721 gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc
    48781 tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa
    48841 ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat
    48901 catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc
    48961 taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat
    49021 aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg
    49081 cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt
    49141 gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa
    49201 aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct
    49261 ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca
    49321 taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa
    49381 aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta
    49441 acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg
    49501 tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg
    49561 ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag
    49621 aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat
    49681 acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata
    49741 tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg
    49801 tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg
    49861 cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt
    49921 cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa
    49981 agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc
    50041 atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc
    50101 aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg
    50161 cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt
    50221 tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg
    50281 acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga
    50341 atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta
    50401 cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata
    50461 tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca
    50521 aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt
    50581 gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc
    50641 tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac
    50701 agtaattact gacgatctag gtttagaatt gaaagatgca acaatcgaaa acttaggaaa
    50761 tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa
    50821 agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga
    50881 ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt
    50941 taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc
    51001 attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct
    51061 agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat
    51121 caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta
    51181 tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc
    51241 tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac
    51301 acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt
    51361 cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat
    51421 gggcggtatg atgtagtaat taagaaagaa ccctatttta acagggttct ttcttctttt
    51481 tttgttttat tttaagcaaa aggggcaaag aaggggcaaa aatatcaaag gctatttagt
    51541 ttatcaatta cttgttgttt ggctcttttt gttacatggt tataaataga cagagttgtg
    51601 tttgcatcag aatgccctac acgctccata atggctttaa gaggtacgcc taattcagat
    51661 aatagtgaaa cgtggctatg tctgaatata tgtgaggata gacttttttc taattccaac
    51721 tcttcctcta ctttatggag tatagcgtta aatgagtgca gcgcaagcgg agtgccactt
    51781 gtagatataa atatatattg atcgggatct gtgggtctac ctgcaagaat attgtcagct
    51841 atcacacttt caatcaattc ttttgcacga ttgggtaatt gcacttcacg ttgcgaataa
    51901 gtattttttg gagttgtttt tatagcatta tccattttca cagatgtgta atctaaggtc
    51961 ccattaatgg aaattttccc atcctcatag tccttcattt gcaaagctag caattcccca
    52021 tatctcaaac cagttaaata tagaaactca gctattatgc cgtgtagttt tctgcgagga
    52081 ttggagtata gctgtttcag tatttgatca atttcttctt tctccagata ttttttattc
    52141 atagaaagcc ttcttttttc ttcttccact tttttaggat ggattttaac tgctagcgca
    52201 gggtttcttt gaatgtattt tctatcgatt gcatagttta gcataacaga tagagttgtt
    52261 tttgtttgtt ttgtgtagtt cagtgagagg tcaccaaacg tatacatatc ttcaattatc
    52321 ttattaatga gtgtctcatc aatgtttcta acgatcgtat catcgcttat gtgcttagaa
    52381 acatgtttca tcatcattgg aaccttcaaa tagctagtac gtttaacatg ctgcttataa
    52441 tattcatacc attctttata cagctcacca aaagtgatat ctgatttatt gtaatcttcg
    52501 agtgcttctt taattttttt atctagaatt ttctgagctt ttttccacgc ttgtggtgaa
    52561 ttacttgtaa gtgttgtaga tttttttcgt gttttttctg tataaggatc tacatatctt
    52621 tcaataaact tgaattggcc atctttggtc tgttcaaccc acacttttaa catctcctat
    52681 catttgctat aataggcata acaaatagac ctatataggt ttgttttcta aaagcacgct
    52741 cttactctgg acggtggggc gtgtttttta tttattatac agttgcttta aaaatagctg
    52801 ctttttcaaa tgatactaaa ggagagaagt ggatttctat ttctcccttt tggtttaacg
    52861 tgaaatgagc agttacatcc atttgttttc ctggagcaac agatcccatt gtattttcgt
    52921 tggcatatgt ctctgatttt ttgtcatctg gtccatatac ttccacatct gtacctacag
    52981 ggatatctga atcgccatca ttttttacgg tataagtaat ttttactact tgtgcaggtt
    53041 ggttttcttc aaattgattt ctttcatcag ttagttctac actatttagt gtatattcag
    53101 catcaccaac ttttacagtg tcaccaatct tatagaaggt atcgcttttt tcttctttag
    53161 aagatgaagc agaggttgaa gatttagtta ctttttcgcc accattatca cttgctttat
    53221 tactatctga tccaccattt aaagcagaac caataattat aattaaaatt actgctaata
    53281 cccaaaacca tacttttttg taaaaaggtt tactaacttt atacattttt ccgtcttgac
    53341 ccataacttt ttttgccatt taaatattcc tcacttcttg ttataatata tttgcgatct
    53401 cagaaatgag gtatgagtcc gtgtgccagc acgggctttt ttaatgtttt gctgaaatcg
    53461 gtttttttaa taattcttga tgtattcata catattgcct tgcgtaagaa tattttttct
    53521 taaaatagca ttggcagaca gcataacaag tgtatctgag cttattctga atagaataat
    53581 actccataaa tttttcgaga ttaaactgag attcatcagt cagttcgttc tcaatataga
    53641 tatttaataa aattagaata gctattttat ctgcttctgt ttcaaatttt gagtgaaaag
    53701 ttgtagaagt atcgtacaaa actgaatatt caaaatgaga agcggtgaaa tgcgcaagtt
    53761 catgagataa atgaaaggct tctgcagttt cgccatatag attttcattc aaaaaaatga
    53821 ttctaggctt cggataatag aagccaggct ctttcatttc catgtaaact actttcaaat
    53881 tgtattcgct caacatttct ttcaatttca aatacataca acccatcact ccaactattc
    53941 attttcctct aaagctttag caattgcaat cgctttacgc attgtctcct tagatatttc
    54001 ttttccatca aaagaaaaaa cagtatcgtt ttctgataaa tccacatgtt taggggtttc
    54061 tctttcttct ctacctagaa ggtagtctac agagactcca aagtaatcag ctaaccgagc
    54121 taactcaata gaattaggag tttgtctttt ccaacttcct aaatatccat tcgaataccc
    54181 gaaattcatt tcgagctgtc taatggataa tcctttttct tttgctaact cctttattat
    54241 ttcgtaagta ttcattgata aatcaacctt tctgaatgct tacaaaaaaa gtttagataa
    54301 ataatgtaaa aatagttgac ttatatacat tattaatcta tactatgttt tgtaaacaag
    54361 tttaacaact aaaaagacaa caaaaaatac tattgattaa taaatgccaa ccgccaagaa
    54421 agctttttaa atcaaagttt atatgtctta tttaactatg catttagtat agattattaa
    54481 tctaataaag tcaacgcagt tttttaaaaa gttgttaaat ttgtttacga atataaaaga
    54541 aaggagagaa gaatatggaa aaaacagtct cggcaaaaat cgaagatttg aaattagaca
    54601 tactgaaaca agcaaaagtg gcgatggaac acgcggtaga taaagaagac tctgccatgg
    54661 ttgcagccat agcagagatt ttagctcacg tttagtcatc gtttttacgg tcttctggaa
    54721 gctgaccata gataacagca taatgaatat ctaaataagc ttgaacaatt tcttttggcg
    54781 aaattgattc gcctttagta gatacttgtg attcgtgatt tagccatgca acaactaaat
    54841 ccgcagcgat ttcaaacggc gttacacttt gtgtcagttg ccataaagaa tttcacaata
    54901 tgtatggtcg tggtaataat accaaagagc aatttgagga atttatggaa attaaaaaat
    54961 acaaaaaatc atttattaaa ggaggctaaa caatgaacaa ggaaatgagc caaacaatca
    55021 aggtacaaaa aatgatagat gatctaacac atggaattga tagccaagcc gataaaatca
    55081 tcaaggaatt gcaaggacaa aaagttaagg atgcaaaaat gcttctcaag actatcaact
    55141 ttgaaatgaa tccaacaaaa agaaagctcg ccgatgtgtt ggaagaaaag ttggcttccg
    55201 ctatcaacga gcaagaatta ctatttgaaa ccgatacttt taacagctga tgttttatag
    55261 atgacagttt catccgaatc tatatcatcg ccgagagaaa agaatgttgc ttttgacaaa
    55321 aagccgataa gacctacaag cggtcttgat gttgcaagag tagatacccc aatatcacct
    55381 tgcaaggtat tcacgtaaat ttgagaagaa tagaacattc catctttttc ttcgatattt
    55441 gacgcatcat cgtatccaac gaccaaagta tcggcaaata ttttgatttt ttctccatca
    55501 agcatagtaa ttacaaattc tggtttcata aattttcacc tccttaacaa ttatttcagc
    55561 ctgtcacact gataaggaaa ttataccaaa gaaaggaatg agaaatatga atacaccaca
    55621 aatttttaat ttcgagcaaa acgaagttcg aactatttta gtaaatgatg aaccatattt
    55681 tgtaggaaaa gatgtagcag acgttctagg atattcgaat ccgcaaaaag ctattcgaga
    55741 tcatgtggac ttggaggata agacgcagaa cgattcgttc accgtcaatg gaacagcagt
    55801 tgttttgatt aacgagtcag gcctttacag tttaatctta aaatcaaaac ttccctctgc
    55861 caaaaaattt aaacgttggg taacgagtga agtccttcca acaattagaa aaacaggtag
    55921 ctattcaaac gtacctcaaa gttttgcaca agcattgcgt ttagcagcgg atttagaaga
    55981 aaagaaccaa ttactcgaac aacaaattgc cgagtacgaa ccaaagatta gctacttaga
    56041 tacgattctt tcatcgacag atacggtagc tacttctcaa attgcagctg attacggaat
    56101 gtcggcaatt gctctaaaca aattgctaaa cgagttaggt gttcagcata aagttagcgg
    56161 acaatggata ctttaccgaa aacatatgaa ccaaggatac acaaaatcgc acacaagtga
    56221 gataccgaaa gccgatggcg gcactaaagt tgtaatgaat accaaatgga cacagaaagg
    56281 gcgagtgttt atttacaact tattaatcgc agagggctat taccctcaaa tggatttaga
    56341 ggaaattggt tagaaaggag ttttagtatg actgacattg cagaaatcac tcaacgagat
    56401 agagaaaaaa tcaaagaata tgtcgaaagt tcaaagttct taacttacac catgcttgct
    56461 gaaagatttg gaattagcaa aagctactta tctttaattt taaacggtaa aaagacttct
    56521 gcagaagcaa acagaattat agattcgatt atcactatgt acgaattgta gagggaggta
    56581 aagtcgttgg aagataaaat cattcttaca aaatcagagc ttcaagaaat gttaaacatc
    56641 gagtatggaa cgataatttt taggagggat aataagtgaa aaaaccaaca ctttcagagc
    56701 tgatagaagc tgctgagaag gcagtaaaac cagacgactg gtaccgacaa agtttaatct
    56761 tggagaagtt ccacggcatg tcaaaaacta ctttagttga atactgcaag gaaatggaaa
    56821 caattcctga atttgcagaa ggaatacttc gtccaggtca ctcaaccaca tttattcatt
    56881 accatacttt tatttggttt ttaaagtgga aagacgcaaa caaatatcgt gtaaaaagtt
    56941 tatcgccttc agatgttttg aaggaggcaa gttaatgggc aaattcaaca gagcactagt
    57001 attcagcgca ccgctaatca tctacgcttt aggcctctgg ggaagcagac aagcgttgat
    57061 agggacaatc gtttacatgg tttggatttt tatggggctt gatgaagctg agcacagagc
    57121 gaaaaaagcc agtcgggagg gactgactaa aatatgattt ttttattttt gttcctttgg
    57181 gctgctattg cgtacttcat ccagctcctt gttgtcaaag ttgctcttat ttcttttagg
    57241 aagaacaaaa gaaaatagcg ctataaatga aagataagta gccgctacat taaactgttc
    57301 ttttgaaaaa gttacagtca tcggaatgat aattgatatt gaggaagtaa tcttaattaa
    57361 aaatatagca atattttcaa catcgcgttg gtttttattt aacattgtcc aacatgtttt
    57421 agcaacaata aaaataatta cacatgatat tagcacacag actgaaaaaa gttttagaaa
    57481 tgcgatgtag tcagtgttat tgaactcctt caaaatacta tttttattca atgtaataaa
    57541 aggaattttt ttaattgaag acaggaaaga taaggtaata aacagaatta tatatatggt
    57601 taaagtcgca gtaagaatgt aattgagctt tttcctcata gaatcacctc actttctaac
    57661 gaaaattata ccaaaaagga gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa
    57721 agaagctgta actacgagct tacaagtggc agaaagtttc aaaaagaaac ataagcatgt
    57781 actagaagca attgaatcaa taaaaagatc ggtcgaaaat tcggccaatg ttgaagatgg
    57841 gtccaatttt ggacagatgt ttgtggaagg gaacgagcca gactcatacg gaagaagtcg
    57901 gagagtttat ttcatgaata gagacggatt ttccttgcta gctatgggat tcactggaag
    57961 taaagcaata aatttcaaac taaaatttat tgaagctttc aacgaaatgg aagatgttat
    58021 tcggaagaat actgttcctc aaacaattga aga
//
LOCUS       DAFCNJ010000013        57233 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000013 DAFCNJ010000000
VERSION     DAFCNJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.013"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            281..973
                     /locus_tag="LNE35_000983"
     CDS             281..973
                     /locus_tag="LNE35_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK6910158.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1181..1471
                     /locus_tag="LNE35_000984"
     CDS             1181..1471
                     /locus_tag="LNE35_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910159.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            2185..3555
                     /locus_tag="LNE35_000985"
     CDS             2185..3555
                     /locus_tag="LNE35_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910160.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(3795..3878)
                     /locus_tag="LNE35_000986"
     tRNA            complement(3795..3878)
                     /locus_tag="LNE35_000986"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3842..3844),aa:Leu,seq:gag)
     gene            3933..4112
                     /locus_tag="LNE35_000987"
     CDS             3933..4112
                     /locus_tag="LNE35_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910161.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            4261..5478
                     /locus_tag="LNE35_000988"
     CDS             4261..5478
                     /locus_tag="LNE35_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6910162.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            5662..6498
                     /locus_tag="LNE35_000989"
     CDS             5662..6498
                     /locus_tag="LNE35_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6910163.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            6574..7722
                     /gene="nagA"
                     /locus_tag="LNE35_000990"
     CDS             6574..7722
                     /gene="nagA"
                     /locus_tag="LNE35_000990"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBK6910164.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            7942..9000
                     /locus_tag="LNE35_000991"
     CDS             7942..9000
                     /locus_tag="LNE35_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBK6910165.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            9004..10038
                     /locus_tag="LNE35_000992"
     CDS             9004..10038
                     /locus_tag="LNE35_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBK6910166.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(10140..10856)
                     /locus_tag="LNE35_000993"
     CDS             complement(10140..10856)
                     /locus_tag="LNE35_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6910167.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10825..11610)
                     /locus_tag="LNE35_000994"
     CDS             complement(10825..11610)
                     /locus_tag="LNE35_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBK6910168.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(11601..12350)
                     /locus_tag="LNE35_000995"
     CDS             complement(11601..12350)
                     /locus_tag="LNE35_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK6910169.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            12648..13172
                     /locus_tag="LNE35_000996"
     CDS             12648..13172
                     /locus_tag="LNE35_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBK6910170.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13188..14687
                     /locus_tag="LNE35_000997"
     CDS             13188..14687
                     /locus_tag="LNE35_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBK6910171.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(14775..15377)
                     /locus_tag="LNE35_000998"
     CDS             complement(14775..15377)
                     /locus_tag="LNE35_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6910172.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            15481..16437
                     /locus_tag="LNE35_000999"
     CDS             15481..16437
                     /locus_tag="LNE35_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBK6910173.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(16742..17698)
                     /locus_tag="LNE35_001000"
     CDS             complement(16742..17698)
                     /locus_tag="LNE35_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6910174.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            17872..18432
                     /locus_tag="LNE35_001001"
     CDS             17872..18432
                     /locus_tag="LNE35_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910175.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            18425..19249
                     /locus_tag="LNE35_001002"
     CDS             18425..19249
                     /locus_tag="LNE35_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBK6910176.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            19259..19876
                     /locus_tag="LNE35_001003"
     CDS             19259..19876
                     /locus_tag="LNE35_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK6910177.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            19894..20577
                     /locus_tag="LNE35_001004"
     CDS             19894..20577
                     /locus_tag="LNE35_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK6910178.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            21260..22495
                     /locus_tag="LNE35_001005"
                     /pseudo
     CDS             21260..22495
                     /locus_tag="LNE35_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            22503..23390
                     /locus_tag="LNE35_001006"
     CDS             22503..23390
                     /locus_tag="LNE35_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6910179.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            23407..24243
                     /locus_tag="LNE35_001007"
     CDS             23407..24243
                     /locus_tag="LNE35_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6910180.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            24246..24869
                     /locus_tag="LNE35_001008"
     CDS             24246..24869
                     /locus_tag="LNE35_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910181.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            24866..26158
                     /locus_tag="LNE35_001009"
     CDS             24866..26158
                     /locus_tag="LNE35_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6910182.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      26517..26614
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            26730..27311
                     /locus_tag="LNE35_001010"
     CDS             26730..27311
                     /locus_tag="LNE35_001010"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBK6910183.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            27314..28621
                     /locus_tag="LNE35_001011"
     CDS             27314..28621
                     /locus_tag="LNE35_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBK6910184.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            28914..29402
                     /gene="purE"
                     /locus_tag="LNE35_001012"
     CDS             28914..29402
                     /gene="purE"
                     /locus_tag="LNE35_001012"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBK6910185.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            29395..30519
                     /gene="purK"
                     /locus_tag="LNE35_001013"
     CDS             29395..30519
                     /gene="purK"
                     /locus_tag="LNE35_001013"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBK6910186.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            30532..31827
                     /gene="purB"
                     /locus_tag="LNE35_001014"
     CDS             30532..31827
                     /gene="purB"
                     /locus_tag="LNE35_001014"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBK6910187.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(32007..32384)
                     /locus_tag="LNE35_001015"
     CDS             complement(32007..32384)
                     /locus_tag="LNE35_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910188.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32640..33368
                     /locus_tag="LNE35_001016"
     CDS             32640..33368
                     /locus_tag="LNE35_001016"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBK6910189.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            33368..33622
                     /gene="purS"
                     /locus_tag="LNE35_001017"
     CDS             33368..33622
                     /gene="purS"
                     /locus_tag="LNE35_001017"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBK6910190.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33624..34295
                     /gene="purQ"
                     /locus_tag="LNE35_001018"
     CDS             33624..34295
                     /gene="purQ"
                     /locus_tag="LNE35_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBK6910191.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            34296..36518
                     /gene="purL"
                     /locus_tag="LNE35_001019"
     CDS             34296..36518
                     /gene="purL"
                     /locus_tag="LNE35_001019"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBK6910192.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36503..37942
                     /locus_tag="LNE35_001020"
     CDS             36503..37942
                     /locus_tag="LNE35_001020"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBK6910193.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37965..39017
                     /locus_tag="LNE35_001021"
     CDS             37965..39017
                     /locus_tag="LNE35_001021"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBK6910194.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            39014..39592
                     /gene="purN"
                     /locus_tag="LNE35_001022"
     CDS             39014..39592
                     /gene="purN"
                     /locus_tag="LNE35_001022"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBK6910195.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39589..41124
                     /gene="purH"
                     /locus_tag="LNE35_001023"
     CDS             39589..41124
                     /gene="purH"
                     /locus_tag="LNE35_001023"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBK6910196.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            41140..42414
                     /gene="purD"
                     /locus_tag="LNE35_001024"
     CDS             41140..42414
                     /gene="purD"
                     /locus_tag="LNE35_001024"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBK6910197.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42536..43450
                     /locus_tag="LNE35_001025"
     CDS             42536..43450
                     /locus_tag="LNE35_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6910198.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(43583..45148)
                     /locus_tag="LNE35_001026"
     CDS             complement(43583..45148)
                     /locus_tag="LNE35_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6910199.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(45141..46592)
                     /locus_tag="LNE35_001027"
     CDS             complement(45141..46592)
                     /locus_tag="LNE35_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6910200.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(46606..47622)
                     /locus_tag="LNE35_001028"
     CDS             complement(46606..47622)
                     /locus_tag="LNE35_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6910201.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            47842..48930
                     /locus_tag="LNE35_001029"
     CDS             47842..48930
                     /locus_tag="LNE35_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBK6910202.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            48984..50510
                     /locus_tag="LNE35_001030"
     CDS             48984..50510
                     /locus_tag="LNE35_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK6910203.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            50512..51717
                     /locus_tag="LNE35_001031"
     CDS             50512..51717
                     /locus_tag="LNE35_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6910204.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            51974..52951
                     /gene="guaC"
                     /locus_tag="LNE35_001032"
     CDS             51974..52951
                     /gene="guaC"
                     /locus_tag="LNE35_001032"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBK6910205.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            53215..54639
                     /locus_tag="LNE35_001033"
     CDS             53215..54639
                     /locus_tag="LNE35_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK6910206.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            55100..56209
                     /gene="pdhA"
                     /locus_tag="LNE35_001034"
     CDS             55100..56209
                     /gene="pdhA"
                     /locus_tag="LNE35_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBK6910207.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            56212..57189
                     /locus_tag="LNE35_001035"
     CDS             56212..57189
                     /locus_tag="LNE35_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBK6910208.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        18451 a         9703 c        12410 g        16669 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacacat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgcttaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactgctg cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg
     1081 attttaagaa aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa
     1141 agggaatgta tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac
     1201 agtagataag atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa
     1261 tggggtcaat tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg
     1321 gatgaagtta gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta
     1381 tcatgtgtta gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa
     1441 aaaggtccga caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct
     1501 agccgcttgt tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg
     1561 ctcacaagaa cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact
     1621 aaactttcca aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc
     1681 ctattagtat tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag
     1741 tctcaggtag tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg
     1801 aagaggtgat tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa
     1861 cgtcctggtt cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct
     1921 tgttcgtcaa ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc
     1981 tgcattactg caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat
     2041 ttttttgttg actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat
     2101 ttaaaagata acttttaagt tatctttaag taatgcttaa agctatgtta tactctatta
     2161 ggaaatataa gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa
     2221 gaactgatct tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg
     2281 ccgactatga gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg
     2341 ggtgcttcga tagaacatta taagacatgg agttcaagaa ttattataga actattctta
     2401 atgttctttt ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga
     2461 attgtagtat tactttgcaa gtatgttttt gagaaaataa atgttaaaaa tttattattg
     2521 gtttgctcta tatactgttt aataccattg acagtaatgg gggaaaccgg ttggagagca
     2581 accaccttga actatcagtg gccagtagca tttagtttac taacttttta tccgtttttt
     2641 caactcttaa gaggagaaga aataaataga aaaatatatt gggtaagtat tcctttattg
     2701 atatttttaa ctaatcaaga acaagtcaac gcctgttttt ttgttctaac aagtatagtt
     2761 agtttatatt tgattgtcaa tggacgatat aattataagt tatcagtatt ttcaataata
     2821 agcttagcag aattaatttt ctctttgaca actccaggga atgcattacg tgctgcacat
     2881 gaaattaata aatggtttcc tgaatataaa aatttcaact ttcttaataa gttagattta
     2941 ggtatttcat catttggaaa gccgtttttt ttagacatga atatcttatt tttattgcta
     3001 tttttcttaa ttttccttct aacatatagg aaatgtcaaa actattatgt gcgtatattg
     3061 actgcattac cgtttttctt aaacctgatt atttattttg gtaatacaat gggtcaaagc
     3121 tttacttacg tcaatggaaa taaaagggca atgatttgga gtagtagtaa cctgaataat
     3181 ctctttacag agttaggaac gaaactgtca cttttttatc caggtacttg gatagctact
     3241 ttagtagtct tggcattatt actatgttta attgttggaa tttatttaag ttttgacaat
     3301 aagaaaactt ctattttttt agttattttg atgataatgg gattttgttc aaggttaatt
     3361 atgggatttt ctcccacagt atgggcttcc ggaatgcgga cgtactatat actttatgtt
     3421 gttatcgcca tcttagtact aatggcagtt aaagagctaa tgaaaagcat gagtgtacaa
     3481 aaaaatgaat ttatgcaatt tggattaacc gtgcttggta tttgtacctt tataataacg
     3541 gtgataaaca gataatatac aaaaaatccc tcgctttagt attaggtgag ggattttact
     3601 cgtttttcga ttaattgttt taacacttgc aagtttctac gagtagcttg tttccgacct
     3661 tcatatactc acctcaggct gtttacatga ttgtacgttc tttttgtaaa ggatacagtg
     3721 atatgtatat aatggcataa agaaacgttc aaacaaaaaa actcgtgaag caaaattaac
     3781 ttcacgagtt gtagtgcaga tgatgggact cgaacccaca cgagctaacg ctcacatgac
     3841 cctcaatcat gcttgtctgc caattccaac acatctgcgt aataataaca cactaatgat
     3901 agcgaggttt tacatagttg tcaagctcaa aaatgggagg acttattaag atggagaaat
     3961 atgacggaga attttcagga ctgggaatga tcttaggtgt attgattgga cttgcattcg
     4021 gacgtttttt gcttggtttt atgctaggaa tcatttgtgg aattgcaatg gattgggcag
     4081 cgaatctatg gaatgactat catgacaatt aagttctcaa cgcttgtttg tgttttttta
     4141 atacttacgt ttttcctaaa taagtggcaa aattttctag gatggagtat gatattgaaa
     4201 tagtaaataa caaaaaattt ggaaagttct aactttttga aatgagggat tggctaagtg
     4261 atgagagaat ttgaaaaatc caacaaatta gaaggtgtca gttacgatgt tcgcggtccc
     4321 gttctagaag aagcggagcg gatgcaagaa gagggcatta gtattttaaa attgaacacg
     4381 ggaaatcccg caccttttgg atttgaagca ccaaacgaag tcattcgtga tttgatcatg
     4441 aatgcccgtg actcagaagg ctactcagat tccaagggga tcttttctgc tagaaaagca
     4501 attgagcaat attgccaact gaaacatttt ccgaatgtga cgatcaatga tatctacacc
     4561 ggcaacggag tgagcgaatt gatcacgatg tgtatgcagg ggttgctgga caatggagat
     4621 gaagtactgg ttccaatgcc ggactatccg ttgtggacgg cttctattgc attagctgga
     4681 ggcacacctg ttcattatat ttgcgatgaa gaggcagaat ggtatcctga tattgatgat
     4741 atcaaatcaa aaataacgag tcggacaaaa gctattgtta tcattaatcc taataatcct
     4801 actggggcac tttatccaaa agaattatta gaagaaatcg ttgaagtagc tcgacaaaat
     4861 aacctgatta tttattccga cgaaatctat gatcgcttag tgatggatgg tctagaacat
     4921 attccgattg ctacattagc gcctgatttg ttcgttgtta cgctgaacgg tctatccaaa
     4981 tctcatcgtg tagcaggatt tcgagtaggc tggatggtcc taagtggaga taagtcacac
     5041 gtcaaaggct atatagaagg gctgaatatg ttgtcctcca tgcgtttgtg ttccaatgta
     5101 ttgtctcagc aaattatcca aaccgcgcta ggcggttatc aaagtgtcga tgagctgctc
     5161 ctgccaggcg gaaggatcta tgaacaaaga gaatatatct ataatgcaat caatgatatt
     5221 cctggtcttt cagccgtaaa accaaaagct gctttttata tattcccaaa aattgataca
     5281 aagcgattca atattttgga tgatgaaaaa ttcgttttgg actttttaca tgaacatcat
     5341 attctacttg tacatggtgg tggatttaat tggaaccagc cagatcattt tcgaatcgtt
     5401 tatcttccta aaatggatga tttgaaacaa acagctaaga aaatgagaga attcctagca
     5461 acctatcgcc aaaaatgatt ttaaaaacca aatttttcag taggagggcc acagacaaac
     5521 acactgaggc tctctttcta gtggagtttt cttaaataac taaagtatat tatttagttg
     5581 caagtctttt cttgaagttt gcgacttcct aaagtaaaat acgaatagaa tgcgaatacg
     5641 catattttgg ggaggatact tatggaaacg ttaatcgagt cctttggaac gatcattaaa
     5701 gagattcgaa aagaacaaaa aatgacgcaa aaaatgcttt cgcatgatat ttgttcacaa
     5761 agtgtattaa gtcggatcga aaataatgag gaactgccga atgtattggt gatgatgcag
     5821 atttgtcaac ggctaggggt aacgattgat catgtgatga atctctctcg aaaaactact
     5881 cggacaaacg ataaattatt tgaactgttg gatagttgtt tccaaaaacg tgattatcgg
     5941 aagttagaac aagtactaaa atcttctgat atttctgaaa atctgtatca agcaactgac
     6001 tttcaacgct attattatta tctcggtgtt tgcgaattca cgttgcgtca aaatatctct
     6061 cagtcccttt attatttgaa agaagcatta agctactcta gccaaaaaga aagaatccat
     6121 gtttctgaca cagaaataca actgattagt tgtatcggaa aaatatatgg tgtggcagga
     6181 aaaacggctg aagcacgcta ctatttgagt cgaagcatcc aactgctgca tcaatcagct
     6241 gatgaacgat caaaaagtga attttcgcaa attttttata attatggaaa tttcttattc
     6301 catcaaaatg aaatcgacga agcgttagaa caggtgaatc aaggaatcta ttgggctcaa
     6361 gagaagaata gctactatta cctcaatgat ttatttgtgc ttaaaagctt aatatacaag
     6421 cgtaaagaag aaccagaaag agcagcattc tatgaaggct tagcacaagc tgtgaaacaa
     6481 atttcaaaaa atttgtaaat ggtgtagacc attcttgact tcttctctaa ataagagtac
     6541 tttagaggta gaaaaacaaa gggagagaat agaatgagaa cttttatcta tgctgacaaa
     6601 ttctttttag cgtcaggtgt caaacatgca ggctatcttg aaattaccga tggacttttt
     6661 ggtgaataca ccaaagaact acctgaggga gaagtcaaca tcattgacca aagcggcaaa
     6721 tggattgctc ctggtttggt agatacacat attcacggct atatgaatca tgatgtaatg
     6781 gataatgatg cagaaggcat caaagtgatg tctgaaggtc tgttatcttg tggagtgact
     6841 tcatttttac caacaacatt gacgtcaagc aaagaacgtt taaaagatgt agctgagaca
     6901 atcggcaaaa tgtatcagga agcaccagga gctaaaatca aaggaatcta ttttgaaggt
     6961 cctttcttca cagaagaaca caaaggggca caaaacccaa gttatttcgg ggatcctgat
     7021 ttggatactt tccatgaatg gcaagaagct tcaggtggtt taatcaaaaa aattgcttta
     7081 gcaccggaac gtcaaggtgt agaagagttt gtcaaacaag tgactgaaga aggagtagtt
     7141 gtttctctag gacacagtaa tgctacacta gaagaagcgc aaaaagctgt tgaagcaggc
     7201 gcaagtgtat ttgtccatgc tttcaatggt atgcgtggat tgaatcatcg cgaaccaggt
     7261 atggtgggtg cgttattatc tttagaacat gtcttttctg aattgatttg tgatggccat
     7321 catgtacatc caaatgctgc gggaatccta atggaaaaag caggacatga tcatgtcgca
     7381 ttaattacag actgtatgat ggccggtggg atgcctgatg gaaattacaa tttaggcgaa
     7441 tttccagtag tagtaaaaga aggaacagca cgtctagaat ctggcaactt agctggaagt
     7501 atattgaaat tgaaagaagc aattaaaaat gttgtagact ggggattagc aacaccagaa
     7561 caagcagtca tgatggctag tctgattcca gcaatcagct gtaaaatcga tgatacatgt
     7621 ggaaaaatcg ctcaaggacg cgatgcagac tttatcgtat tgaaccctga tatgacattg
     7681 gaagcaactt atctagatgg cgtggaacgt ttccacgcat aatataattt aagttaacct
     7741 taaaactaaa cttcttaagt ttggttttaa ggtttttttg tgttctagta aaaatcactt
     7801 tacataagtt tcaaaagttt ctttaatttc ctattgttca ataagtaaca atgtaaagat
     7861 aaaattaaaa atgttaatac gtattagcaa aataaaagct ttttttattg tgaataaatg
     7921 aacataatta ggaggagaaa gatgaactta tttgacattt ttcgatcgac tttgacagat
     7981 atgtcgattg tcagtgcaat cacttcaaca gtttttatta tattattagg gtttgtttgt
     8041 agaaaaaagg ggattttttc tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca
     8101 gtggctttgc cagcactggc atttcgagct ttcatgcaag atatcaatac agaattactg
     8161 atgcaaggaa tcaatgtctt gatttgggga ttagttattt atattattct tattctagtt
     8221 tcaaaacctt ttttcttaag ctataaaggg gataagcaaa caacgttgcg tgtcctgacg
     8281 atctttggtt ctacgacctt tttcggaacg cctatcgtca gtgcgattta tggtcctgtc
     8341 ggcgtgatgt attcctcaat tttcaatatt ggctaccgaa tctttttgta ttcttatgga
     8401 tacataaaaa tgagtggctt gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat
     8461 ccaatcatta tcgctacttt tgccggcctt ttcatttggc tgttccaagg gtatctgcca
     8521 caagtgtcag ttacaggtgc agaaggagag ataagccaag tagcgttttt gcgaattgac
     8581 cagactgcac cgtggctatt tcagccgatg gattatttag ctggtttaag ttcaccattg
     8641 gcgtggctag caatcggcgc aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa
     8701 aaaacttcat ggtattacag tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg
     8761 attctagcag ttttgacggc aacgaatatt ctaccagtca attttgaagc actggcaaca
     8821 atcgttatca tgatggctac accagctgca acagtggcag cagcctatgc tatcagtttt
     8881 gatagggagg cacttttagc ctccaatgca tccttactgt caactatttt tgctgtggta
     8941 ctgacacctg tatggatcgt tgtattacaa atgattcatg gcataggttt attttcataa
     9001 gaaatggagg aagaaagtat gagaatcgca tgttacggtg ttcgtccgaa cgagatagat
     9061 ttttttgaaa aattgaatat ttaccattat gatctcagtc tttatgaaga actgttgaca
     9121 catgacaata tcgtgtcagc aaaggaccat gatgctgtat tgttgagggg aaattgtata
     9181 gcagatgaga ccaatcttgc aaaaatgcag gaatatggga ttcgttatgt ttttacacgg
     9241 actgtgggag tcaatcatat cgatctacaa gcggcagctg attttggtat gacagtagcc
     9301 cgtgtaccat cgtattcgcc aaatgcgata gcagagttat ctttgacttt tgccatgatg
     9361 cttttgagaa atactgcgta tacgacgatc cgaacatctt tcaaagattt tcgagtggat
     9421 gagcaaatgt ttagtcgtga aatcagaaat tgcacagtag ggatcatagg aacaggcagg
     9481 atagggttga cggaagctgt tttgtttaag ggactaggag ccaaagtgat cggctacgat
     9541 attcatcaga cagaagctgc taaaaaagta ttgacgttcc actcgcttga agagttatta
     9601 gccaaaagcg atattgtcag tattcatgta cctcatatcc caggtgaaaa cgatcaaatg
     9661 atcaatgaag gatttttagc tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt
     9721 ggagagcttc aagataatga agctatttta agagctttga aaagcaatca tttagctggt
     9781 tttgcaaccg atgtttttgc aaatgaaaca gaggtttttt tccgatcctt caagccatgg
     9841 gagacgattc cagaccctgc tatccagcaa ttagtagaaa tgtaccctag agttcttgta
     9901 accccgcatg taggttcaaa tacagatgag gcattatcaa atatgatttc gacaagtttc
     9961 gagaactttc gtgaaataat agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa
    10021 gcccgtcaat taagataaga aaacatggat attcgtcaat aaaatgttta aaaatagtcc
    10081 aagtaaacag ttgttacttc tgtatacttg gactattttg tatgggataa ttcgatgact
    10141 caacagtaat ctgtgagctt tttccgccca ttctttgtca attcataaca ggttagcggc
    10201 tggccgattt ccaaatattt gattctttcg ttcaatatat cttgttctac taaaaaattc
    10261 aaatatttct tgacggatat acgtgacagt gagctttttt gagccagttc ttttgttgaa
    10321 aaaatagtat cttcttttag aataaatgca cttatttttt ttaaagtcag ttttgacaga
    10381 cctttaggca aatcgatgaa tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact
    10441 tgatcgatca atttctgatc ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga
    10501 tacttttcga tcgccagctg aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag
    10561 gcaccgtagc ggatcacatt ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc
    10621 actgggatat catttccttg ctgtctaagc caagataaaa aatggatacc actttcgcca
    10681 ggcatgtata catcaagcag cagcagatct gcgttttttt gtctgaaata tttttttcct
    10741 tcttctacat ttctgaaact ttcgattacg ttcatattag ggatgctttc cacaaaagta
    10801 cgattcaatt ctgcaaccat tggatcatct tcgattatca ggacatttgt catcattttg
    10861 atcttccttt tttggtaatt ctagatagac gattgtaccg ccatttgttt gtttggaaaa
    10921 ttctaaaaat ccaccgtggc gtttgacgat tgaatggatg agattcaatc caaacccacg
    10981 attgattccc ttcgtcgaaa aacctagcgt aaatattttt tctgagattt cttctgatat
    11041 cccgacaccg ccatcgcaga cggtcacgat gaatacttca gcttctgtgt catagtttaa
    11101 atgaatccgg acctgtccgt ttctttcatg tttttgtagc acggcttctt ttgcattatc
    11161 aagtaagttc cctaacactt gcaaaagtga atggatggag tcatccatct ccattttagg
    11221 gataaaagaa tcgctttcta acagcatttc aacagattgt tctttggctt cgtttatttt
    11281 gcctaataaa aaaccggcaa ttgcaggtac tttgatcaga cttgagactt ggccaatctc
    11341 ttttttataa ttttgattta gctgctggat aaaatgggtg acttcttcat acttcttcaa
    11401 atcaatcaaa ccagaaatga catgaagttt attcatgaat tcatgggttt gagctctcat
    11461 ggagtttata tattgtttcg tccccccaag ctcctccatt aatttcatta cttctgatcg
    11521 atttcgtaaa gttgcgactg ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct
    11581 agaaatagtt tctatcccat ttaaatacat ggattgagtc cgaacggtcg tttgatgctc
    11641 aatcgtctct ttaaacaaaa cgtcatatag attatcactg atttttttcc tacttcgaaa
    11701 tccaatagct gctctgtttg gtagaaaatg ttttttgcag gtgtattgat caagaggata
    11761 gttttatcag aagagatggc taagattcct tcatctactt cattttcgat aatcgttttt
    11821 tcacttaact gttctgctat ttcttgtggt tcaagtccaa gcagtacttt tttcattttt
    11881 tgggcaagaa aaacggcgcc tatgatgcct acgctaagtc caataatcaa tgaaaaaaca
    11941 atcatttttt gtccatcttt gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa
    12001 actgcaccaa taatctcttg atcataataa atagggacga aatagcggaa gccgaaccct
    12061 aacattcctt ctttttcaga gaaaaattct tgtcctccaa tcactttttt cacatcttca
    12121 tgacttgaga aaggaagacc gatgtaactt gggtctgcgt gagaatatcg aatgccttca
    12181 gggtccatga caacgataaa atctaaattc gatgcaatca tgatttcatt tgttattcct
    12241 tgaatcgcat gtgtcgggtc accagtctgc agcttttttt ggatgagttt atcattggca
    12301 acgattttag caatgctaga gattttatct gacacactag atcggttcat cgaaagaata
    12361 aaattcctga tcaatatggc agaaacaatc aaagaaacga atgtggagaa acaaacgaca
    12421 aggatgatcg tgttctggag ctttaatggt tttttcttaa aaaaaggaaa cataggctac
    12481 ctcgaaagaa tttattgtga aaattttaac atgaaaacaa aaatttaaca aattgtcaac
    12541 gataaacaca accttcctat aaaaaacacc actgagtcgg taaaagaagc tatacagaat
    12601 tttcaaacat tggtatgata gacgtagaaa tggaaggagg cgtgtatatg atagggggaa
    12661 tcgcacttga gacgatttat tggtatacat tgctgatttg tgcaggattg gcagtcctat
    12721 tgattatttt tggagatgta ttcgattttg atggcccgct tgatccgatg cttattatcc
    12781 cttggttaac tttcacaagc ttatttggtt ttctaggtga gcgtttaaca gatcaatcat
    12841 ctttattttt atttattgct agtggaggca tatcgatagt gttggtattt ttgcttaatt
    12901 tctatatatt gatgccgatg aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc
    12961 tggaaggaca agtagctgtt gtgatcacac cgatccctgt acgaggtatg ggagaaattc
    13021 agttgaaaag tgttaccggt tccattagtc gtccagcttg tttttacagc ccccaagaac
    13081 agacagctga gcggggaacg aaagtattga tcattgagat caaggaaaga atctgttatg
    13141 tcatgcctta tgaaagcatg ataaaagctt aaaggagcgt gtgttaaatg gaaataccag
    13201 ttgtttttat cgcgatcgca atcgcagctt tgattttact gatgttgctg atcgtctttg
    13261 tagcgaagta ccagacagca aaaccggatg aagcgttgat catcagtgga agttatttag
    13321 gaagcaaaaa tgtgcacatt gatgaaagca ataacaaaat caaaattgtg cgtggaggtg
    13381 gcgcattcgt cttgccagtt tttcaacgtt cgaatcggat cagtctatta tcgagtaaat
    13441 tggacgtctc cacccctgaa gtgtataccg aacaaggcgt accggtgatg tgtgatggta
    13501 cttcgatcat caaaatcggt tcatctgtag aagagattgc tacagcagca gaacagtttt
    13561 taggcaagac aagagaagaa ctggaaaatg aagcaagaga agtattagaa ggacacttgc
    13621 gctcgattct tggttctatg actgtagaag aaatctatca aaatcgcgat aagttcagtc
    13681 aaagcgtaca agaagtcgct tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt
    13741 tcactatcaa agaggtcaga gacaaaaacg gttatcttga ctctttaggg aaaccaagaa
    13801 tcgcccaagt caaaagagat gcagatatag cagaagcgga agctctaaaa gaaacgcgta
    13861 tcaaaaaagc tcaagcagaa aaagaatcac aagcagctga attacagcga caaacagaga
    13921 tcgcagaatc attaaaagaa aaagaactga aattagcaac ctacaaacaa gaacaagatg
    13981 ttgctaaagc aaaagcagac caagcctaca atttagaaag tgcccgagca caacaacaag
    14041 taatcgaaca ggaaatgcag attaaaatcg tagaacgcca aaaacaaatc gaactggaag
    14101 aaaaagaaat cacacgtcgt gaaaagcaat atgattcgga agtgaagaaa aaagcagatg
    14161 ccgatcggta cgctaaagaa caagaagcac aagctcaaaa agtcaaagaa gtgactgaag
    14221 ccgaagcaga acgattccgt gtggaagcac tggcagaagc ggaagccaat aagacacgtt
    14281 tagctggaca agcagaagcc gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa
    14341 aacaaaagat cgccaatgcc ttcaaggaat atggcgaagc agctgtcttg agtatggtta
    14401 tcgatatgct accgcaattg atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa
    14461 tctctgttgt cgatacagga agcagttcag gagaaaccag tggagcgaac cgagtgacaa
    14521 attatgccac aaatttgctt tcttctactc aagaaacact aaaagcgaca acaggacttg
    14581 acttgaagga aatcgtagag aatttgtcga cacgtgggac tactagccaa gtaaatatgt
    14641 atgaagcgcc aaaagaggca gaacaaaact cgatgaagga agaataaagt agtacacttc
    14701 accgttcaat caaaaatgga ggaagacttt ttaaagttct tctctccatt tttttatttt
    14761 attgcaaaag aaaattagtc ctcgctattt ttcttttgtt tttggatctt agcttctatc
    14821 tcttgtaaat ttgttcttag caatcgttct ttcttttgga gttcgaccaa atcttctgat
    14881 agttcttcct tcttttctgt ttcaggagga cgaagaaaga aattgatacc gattgcaatg
    14941 aatgtcccta taaaagtatc tagtacacgt tggatagcat acaaagcgga ttcgccttga
    15001 gggatgctca acgcaatcag caacaacgta gctattgcgg aaatgatacc ggaattattg
    15061 ttaataccat ctgacacgac gatcacaata ataactagaa taggaagcaa gatcaattct
    15121 accaaaaaat cattgtgaaa atagcttttg acaaaaaaat aaatgatagc taatgtacca
    15181 cctaatgtat tgccgatgat cctagacttt ccaaaagaaa cggttgtagt caagtcctga
    15241 cgcaaagaaa aaacagcaga taatgcagca atcatcggga ttcctcgttg agtcaaatga
    15301 aaaaacaaga tacataaaaa aacagctaag gcacttttga tcgtacgcat acctagtcga
    15361 aaacggccaa attgcataga gattctcctt tttggtcttt ttactatcat acctaaactt
    15421 ttgtctgata gctagttttt cattattcaa gtataattaa aataaaaaag ggggaagtaa
    15481 atgcgtatag gaatgcgaac agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca
    15541 gcgggtagct tatcgctgtt gtattggcca gctgcaggga tcattgcatt attaagtgta
    15601 ggaaatacca agcgctctac attgcgtacg ggcatgaatc gaatcgcagc ctttatatta
    15661 gctacggcaa cagcactgtt cagttttttt gctgttgggt atactatttt tggcttcagt
    15721 ctttttttat tcctgttcat cccgcttgcc tctcgcttca aattaaccga agggatagta
    15781 gtaaattcgg ttttggtcac tcattattta gtggaggaaa acatgtcttg gcagctgatc
    15841 ggcaatgaag cgggattaat ggcgattgga ttagtatttg ctttgcttgc aaatgtttac
    15901 atgccagatc gtacgaagca gctgaaggaa aaccaaatac agatcgaaaa agaatttcga
    15961 aatattttga gacaaatggc agaatttctt ttatcagaaa ataaagaaga tgtgcagatc
    16021 aaatgcgaac atttactgac attcattcgc gaaagccaag aagatgccag agaacatcaa
    16081 gaaaactact ggctcagaca gccgctatac tacgaaactt acttctctat gagaagagca
    16141 caggccaatg tgattaaaga catgctggaa aatctagaga gaatccagca accagcttat
    16201 tatggaaaac atatctatgg tttattgatt tatactgcag aaacattttc agagtcaaat
    16261 gatggcagac aaattttaac acgtattgaa gaagtttatg tattgtatcg gcaaatgcct
    16321 ctgccgacta gtcgccctga attcgaggat cgagcagaat tgttccagtt tttgcagtct
    16381 tttaaaagtt ttattgaaat caaggcagaa ttctcccaac aaaaaatcca aaaatagagg
    16441 ttgtgaattc agctaatatt caggtcaaaa agctgatttc acaacctctt ctttaaacgg
    16501 tgttctcctg tcaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
    16561 ttttgaaatt gctccttgtg actcggagct gaacggtagg ctcacaacct ctttcttaaa
    16621 cggtgttcaa aagctttcct ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc
    16681 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca accttttaaa
    16741 attacatatt tttccataga acttgcattt gatccaagat ttcgatgaca tcttttgtca
    16801 agaagagaga gcgattgggc aagtcacctt caatcatgtg taccatattc tttatttcgt
    16861 aattcattgc ttcagatgta ttcccgcttt cgatgaattc ctttgtacca tctgtaaata
    16921 agatctccgc acgatcagca cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg
    16981 caacgatacc gacttttggc atttttgcct gaaaagtcaa gctgacagca gcaagttcat
    17041 tttctttgtt acgcaagata gtgacggatt gttcgtccac gcctgtttca aaaggaacca
    17101 ttgtagaggc aactacttca ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat
    17161 acgtaccgat atccaacaga gctcctccag ctaattctgg attgaagaac cggtttttag
    17221 ggtctggttc tttgtaactg ccgaaaggtg cttggatcat ttttaaagcc cccagttttc
    17281 ctgtatccat tattgagcgt aactgctgat aaagtggcat gttgaagatt gtcatcgctt
    17341 ctgctaagat caaattcttt tcttctgcta agcgcatagc gtcagccagt tcttttttat
    17401 tcatcgtgat ggctttttca catagaacgt gtttgcctgc ttccaatgct gccaagatat
    17461 ggtcgatatg ctgtctattt ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta
    17521 acatttcttg atagcttccg taagcttttg gaatattgta acggtgagcg aagttttccg
    17581 ctttttctga agttctggaa gcaactgctg ttagttcgct cgtctcttga ttgaagcttt
    17641 cggcaaactc atgtgcgatg gtaccaagac caacgattcc ccattgatat tttttcatct
    17701 gaaaacgctc cttcatagtt ggattgagtt tcttgtctgt ctattattat aacaaaaatc
    17761 acgaagaata aagaggagag gacttgaaac attaaaaaaa atgaaagcag cgatttaaga
    17821 ggtgcttttt gatcaaaact taagtagaat agttaaaagg aggggataaa cgtgcatcaa
    17881 gtatatattg atcttattat gagtgtatta ttagaacagt ttgaaactga aaaaaatttt
    17941 ttagatgaat acttgctgat cagtccagaa caatggcaaa aatggaaaga tggcgagaaa
    18001 aatctgccat ctgaagcgat gcagaaagta aaaaatcttt tcactgatta tgaatggatg
    18061 ctaacgcaaa agattttgcg tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt
    18121 gcagagtaca agcaaatcaa aacacgcatc gcgcaaaaat ggcttagcag caaagcagca
    18181 aaagtcgagc tgatcccttt gaaaaataat cagcaggctg gtagatacct tgacttgaaa
    18241 gtaagtattc aatatgatga atggggctat gatgatattt tgaattttcg tctgccagca
    18301 gatgttcaaa aacagatcga aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat
    18361 ctggaagaaa cttacgttca tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt
    18421 tcaagtgaat aaatttttag gatttggagc agttgtgatt atgatcgcag caatgggcta
    18481 tgttattttt agcgatgatg attcgcatgt tcaagccggc aatcaagcag tcgaacgaac
    18541 aagcaccaaa acaactgcta gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa
    18601 ggacaaagaa acaaaagatc gtacgtccaa acaattgcca gatgttaaaa cagacgattg
    18661 gaagctagtt ttggttggtc cagatcacaa attagaaaaa gaaattgatg agtctactca
    18721 attggcatca ttaagtaacg gatacttgat cgacaaacgc attgctccag aatatgaagc
    18781 atttgccaag gctgctgaag cagctggatt tccattagtg atggtctcag cttaccggtc
    18841 tgtatcatcc caacaacaag ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg
    18901 tgtatcagaa gaggaggcga caaagattac taaagaaacg attacggagc caggatacag
    18961 cgaacaccac acaggtctgg cagtcgatgt cgtcgatcaa aactggtaca acagttatcc
    19021 ttctactgta ttggatgcaa gttacggaga tcagcctgga gctaaatgga tcgcagataa
    19081 tgctgcgcag tatggattta tcgttcgtta tccaaaagga cacgaagata taactaagat
    19141 cacatatgaa ccttggcact tacgttatgt cggtaaagaa aacgctgagt atatcaccaa
    19201 acaccaattg acgctagaag aatatctgaa tgaattaaat gaaaagtagg aattgatcat
    19261 ggcgaaaaaa aagtggaaaa agcaaaagcg caagaatatc cgttggccag cagtggttgc
    19321 cggtgtagca gtgatactct tagcctttgt tttttccttg aagaatctgt cttctccttt
    19381 tactgataat cagcaagaaa atgaagcgca atcccatcag caatttattg aacggcttgt
    19441 tccgcatgcc caagaactac aagatggtta tggagtgctt cctagtatca ttttaggaca
    19501 agctatatta gaatcaaatt ggggaaaaag taccttggct agcaaataca ataatctttt
    19561 cgggatcaag gcttatgggg atcagaagaa agtaagcctg gaaacaaagg aatttgttaa
    19621 cgaagaatgg ataactatcc aaggagactt caaagtgtat gattcatggg aacagtccat
    19681 ggatgaccat acacaactgt tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa
    19741 agtgattacg gccaccaact atcaggaagc cgcacaagca ttgcaagatg caggttatgc
    19801 gacagacccc ggttatgcgc aaaaaatcat ccaggtcatc gagacgtatc aattgaatca
    19861 gtatgaccgg agatagcaaa ggagaagtgg ctgatggcag aacaatttac gccagaaatc
    19921 atgacggaat tgagaaaaga tatcgtaaga gtaccggaaa tcattttaga agcaagcggg
    19981 atcaagatat ttggaaaatt gatccgttcg atcatcttta ctacggatat tgcgatcata
    20041 cgtaatacaa atgcgaatgc agtaattgct gtgtatcctt ttacacctca tccagcgatt
    20101 actaaaagca ttattgaggc agctgacatt ccagttttct ccggtgtagg tggtggtttg
    20161 acacaaggtg ctcgttcttc ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg
    20221 gtcgttttga acggtcctac gcctttagag acagtaaaag aagtttgtcg agtagttgat
    20281 attccagtta tcagtacggt aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct
    20341 ggtatttcga ttatcaatat cagtgcaggt aaagaaactg cagaagcagt acgccatttt
    20401 cgtaaaaaat atcctgaact tcctatcatt gcgacaggtg gtccaacgga agaaagtatt
    20461 gctgagacca ttcaagcagg agcaaatgcc atcacatata cgccaccaag tagcggagag
    20521 ctttttagtc gtaaaatgga aaaatatcgg gaaactgaga aaaagaatca tgaataacag
    20581 gtgagaatca aaagacgagg aagagcagat agtggctctt cctcgtcttt tgtactccag
    20641 tactatttgt tcagaattca aaaaaaagag aaattttttt gtttttttac ctgtaatgta
    20701 cataagaaga gttaaaacct tttgttatta ttagcattct aacaaaaaaa tgtcttgatt
    20761 tattgaagcg ctttctatag aatgaaaaac gaataaaaac taaaaaaggc acccgacaat
    20821 ttagtagcag ctaaattgtc ggggcttgaa aagtcagcgg gaactgacca atcaagttgc
    20881 catagtcaat gcttgtgcat cgacgtgtat attgtaccta atttcataca gaattactag
    20941 ttatttttct gttttttagg gaagagcgca cacgaagaag catgcttatt tagctatgtc
    21001 aaggatattg ggcagttgtc tgatatcctt ttttctttgc gtaattatcc tcccatcatt
    21061 gaagcaagaa gtgtttttag cgatgaaaga agaatcagca ctatctaatt tttcttgaaa
    21121 gtattgagtt gttcatccta ggcagaacga taaggcaagt cttggttttt attccccttg
    21181 aaaaagatcg tttaataaat atgttctgta taaaaataca gtatattgaa aaataacaat
    21241 ggaagtagag gtgcagagaa tgaagaaaag aatgagaatc atcttagctt taagtgttat
    21301 gttattcttc ttgacggctt gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc
    21361 ttggtggggt tcgcaagtcc gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga
    21421 aaaaaatccc catgtgacga tcgattatga attttatgat tatgaaggat attacacaaa
    21481 attaggtaca ctagcagcat cagatgatat ctgggacatt ttccaactga acaatcaatt
    21541 tcctcaatac atcaatcaga ttgaaccatt agatgaatat gtggagcaag ggataattga
    21601 tatttctaat gcagatgaat cctatttagc aacgacgact tacgacgggc aattagttgg
    21661 actgtctaac ggggtgaatt cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt
    21721 aaatatccct gaaccagctt caaattggac atgggatgaa tttgaacaaa tcagctatca
    21781 aatcactgaa gaattaggaa tctatgctat ttcaagattc gaagatttta tagctggctg
    21841 tattattcaa ataccacaag ttgagaaagg attgaatttt tataatcggg aagatctatc
    21901 taacagcttg ggatttgaaa atccagacta tctaacagat tttttccagc tgcgtaaaaa
    21961 tctggtagac aaaggggccc atccagaacc aggtggtgga gctgcaagta cgacggatct
    22021 gtcttttcag gcggttaggg atagagaagc ggcaatggtg tttctatcaa gtaaccagtt
    22081 gacagaaata ttgtcagggg ctcctgaaga tatggagatt cagttgatta atccacctcg
    22141 cagaaaagca gatggagaat ctggtatccc tttacgttct tcgcaaatgc tgagtatggc
    22201 aaaaagctca gcccataaag aagaagctgc taagtttatt gatttctttc aaaacagtga
    22261 agaagccaat gaaattttaa aaggagaacg tggtgtacca atcatgtcga atattcgaga
    22321 acagctcgct cgtgcagatg atgcaacttt gcaagcgaca tttgattatt tagaaatgat
    22381 ttccgatatg gacaacggcg aagtgaatgt ctttgatagt gctttgaatc cggaaattca
    22441 agaccaatac aatcttttag ttgaaaaagt catttatgaa gaagtaacag cagaaggaag
    22501 gcatgaaaaa agaaaaacta aaacggttct tggatcaaga caatgtggtc ggttacgtat
    22561 ttgcttctcc attcatccta ggatttctat tgataacagt gataccgatg atcttgtcac
    22621 tttattattc gttcaccgat tatcgtttgg gaaatccagt ctcatggatc ggcttggata
    22681 attatataaa gatgctgagt gatgcccgtt ttttgaactc catccgagtc acttttctct
    22741 atgtatttgc atctgtacct gtgaaactga tctttggtct attgattgct tatcttttaa
    22801 cgaggaatat caaaggaacg actttctttc gttctttgta ttatgtgcct agtttgatcg
    22861 gtggcagtat ttctatcgct ctagtttggc gggagttgtt ttctaaaaac gggttagtga
    22921 atattttcct ggaacgatta ggcgcacctg cgctttcctg gtttagcgat caaaaactgg
    22981 tcatgatccc tcttgttttg atgtctgctt ggcaattcgg ttcggcaatg atcatctttg
    23041 ctgcaggtat caaagagatc ccaggctcgt attatgaagc ggcagagatc gatggagcga
    23101 acaaatggca gacgttttac aagatcacgt tacctcttct ttcgccgatc attttgtata
    23161 acttggttat gcagacaatc gcggcgttta tgacttttac gcaggcattt atcattaccc
    23221 aaggtggtcc gaatgatgcc acgaactttt atgctcttta tgtgtataac caagcttttt
    23281 cttttaattc tatggggtat gctagtgcta tttcgtggat catgctgttg attatgggag
    23341 ctgtcacctt tctgattttc aaatcatcaa aaaaatgggt cgttgtgtga atgagaggag
    23401 gaagaaatga ataatacagt aacgaaatgg tttcaatacg gttttatttt gctattaggt
    23461 ctagtcatga tttatccggt cttatggatg atcgcaggtt cttttaaaac ggatcaagag
    23521 attatgagtg gttcgctgaa tttgattcca gagacttttc aatgggaaaa ttacgcaaaa
    23581 ggatgggcgg ggtttgctgg aatttcattt tttactttct ttaggaattc gtttgtcatc
    23641 tctgcagttt cgacgatagg gacagttctg agctcgactt gtattgctta cgcattatcg
    23701 agaatcgatt tcaaaggaaa aagattctgg ttcgtcgtca tgttagtaac gatgatgatt
    23761 cctgcacaag tgattttgat tccgcagttt attatttaca atcggctgaa tttggttggg
    23821 acgtatgtgc cgctgatttt accacatttt tttggacaag catttttcat ttaccagatc
    23881 atgcaattca tggtcagcat ccccaaagag ttagatgaat cagcgattat tgacggatgc
    23941 agcaagtata gtgtctttac caaaattatt tttcctttgc tgaaaccttc gatcatcacg
    24001 acgatcatca tccagtttta ttggaaatgg gacgacttca tgggaccatt gatttattta
    24061 aacaagccga gatcttacac ggtatcgatt gccatcaaat tatttgcaga cgctggatct
    24121 agtacgactt acgcgagtat gtttgcgatg tctacgttgt cgctgctacc ggtttttctg
    24181 attttcttat tcttcaataa atatcttgtc caggggatca gtacaagcgg tctgaagggc
    24241 tgaatatgaa aaagccaata gaagagatgt ttctttacaa gctcaactac tatgtctgtc
    24301 ggattttttt aaccagtctt atcagtaatg ctcttttcgc tttagttcta ggatgtttgt
    24361 tcactttagg attagtgctg tctgctcgtg ttgtctatct gatttatttt ttgatgatgt
    24421 atccttctta ttatctatct gtccattact tggcagaaaa aaaggtatgt tggaggaaag
    24481 ggaaaatcaa acggatgtgt ttcatcatta cgttgtttca gctgtttgtc attatttttg
    24541 cagttgattg gtatttatta gtgggaaaga ttggagcttg gtatctgatt ccttttctat
    24601 tgtgtctgtt gctttttgta tcccatagtt tttttagtac gctaattgat atctgcaaag
    24661 aagataaagc agatagtctt gtttatttga tcaaaaggaa tatctggctg ttgtcaaaaa
    24721 acgtcaaggg gtttctgtgg tatttatgcc ttttgtatgg actgttctgg tgtatacagc
    24781 ctgatttttt actcactgca ttactgtatc cagggctgcc atttgtcaca gtattttgct
    24841 taaaagaaaa ggagaatgac tttaaatgaa gaaaaaatat gtacaagtcg gacttggtgg
    24901 gagagcgcgt tttttttatg aggcaattgc ctctacgtat catcaaactt gtgaactagt
    24961 cggtttttgt gatgtatctc aaacaaggat ggattatgca aatcggatac ttgtgaacaa
    25021 gtatggacag aaaaaagtaa agacatgtat gccatcacaa tttgagcaac tactggatga
    25081 cgtacggccg gctgaagtca ttattacgtc agtagatcga acgcatcatg attatatcat
    25141 tcgtgcaatg aaaaaaggat atgatgttat ttgcgaaaaa cctctgacta tcgatgagga
    25201 aaaagctcaa gaaataataa cggtccaaaa acagacggga agaagcctgc gagtcacttt
    25261 taattatcgt tatgcaccgt atcacacgaa gataaaggaa ctgatagaaa gtgatgtgat
    25321 tggtgatgtg ttttctatcc acttcgaatg gctgttaaat acgcaacacg gggcagatta
    25381 tttcagaaga tggcatcgaa ataagcacaa tagcggcggg cttcttgttc ataaatctac
    25441 ccatcatttt gatctagtga atttttggtt gaacagccgt ccccaaaagg tatttgcatt
    25501 cggtggatta aacttttatg ggatgcataa tgcggagaaa cgcggagtag aaatatttta
    25561 cagtcgatgc tatggaagcg aactagctaa agctgatcca tttgctatag acctgtcagc
    25621 caatgaggaa ttgcgagaaa tgtatttaga agcagaaaaa gacagtggat atattcgtga
    25681 ccgcagtgta ttttccgatg atatctctat tgaagataca ctaggcgtaa cggtcaaata
    25741 tgaaactggt gcgattttga cgtattcgct aaacgcgtat atgccgtggg aaggattcaa
    25801 tgtttctttc aatggcagca aaggacggat cgaaatgaaa gtgatcgaaa agtcttacat
    25861 caatgcaggt ggagataaaa aggatgaagg agcagccgtt cttcagtcca tccatgtcta
    25921 tccgatgttc ggtgagccat atgaggtaat cgtcgaacaa gaagcgggcg gacatggcgg
    25981 aggagatccg cgtttattag atgatctatt tggtgaagaa aaagaagaag atccgtggaa
    26041 tcgggcagca actcacgtag atggtatttt gtcgattttg acaggaatcg cagcaaatca
    26101 ttctattgct tcaggaaaag cagtagtgat tgacgagctt gtcagcttaa aagaataaga
    26161 atagatttac ggaaattact tcccgtagtt tataaaatct tgccttattg ccgataggct
    26221 gtcttttgaa cactatttat tcggaaaaag gggctatgac atgctttttg tcatgccttc
    26281 tttttatgtg ggctgctttt ttagatagga gaaaataaaa ataactattt aaacccgaac
    26341 aaaaaggaat aatgcattaa aaatcattag attttacaaa aaaataagta aaccacgttg
    26401 tcagtttcag aaagtagtgt tagactgttt tcaatatttg aaacaacatt ttttattcaa
    26461 atatggcaaa ctgccttcac gtgcaaaaca gttgcaagaa aatgaattta cactattatt
    26521 agttttacat acctacttat atatcgtcat aatatggatg acagtttcta gccagtaccg
    26581 taaatgctgg actataagta aaagattggc tatttcaccc ttcttttctt atgataaagt
    26641 ggagggtgtt tttctttaac agaaacatag taggtttttg cacaaaagcg gttatttcgg
    26701 tattaataaa tgataaagga gcaaataaag tgaaagaatt agttgaacgt attcaaaaag
    26761 acggacgtat attaggcgag ggcgtattaa aagtcgacag cttcgtcact caccaagtag
    26821 accctgtatt gatggaacaa atgggaaaac ggtttgcaga agtatttaaa gaacaaggaa
    26881 tcacgaaagt tgtgaccatt gaagcttctg ggatcgcacc agctttattt gctgcaagag
    26941 aattagatgt tccaatgatc ttcgctagaa aagcaaaaag tttgacgatg gatgaagaat
    27001 tattgacagc ttctgtttat tcttttacga aacaagtgac aagtacgatt tctatttctc
    27061 gtaaattttt atcttcagaa gacaaagtat tgattatcga cgatttcttg gcaaacggac
    27121 aagcggcaaa aggattgatc gaattatgcc agcaagctgg agcgcaagta gaaggaatcg
    27181 ggatcgtcat cgaaaagtct ttccaagatg gacgtcagtt acttgaagaa atgggattga
    27241 gagtcgtttc tcttgcgcgt attgcttcct tgacgaatgg acaagttgag tttcttgagg
    27301 aggacgcata atcgtggaaa aagaaacaca aaatggaaaa gctgcggttt tagggctgca
    27361 gcatctgcta gcgatgtatg caggagcagt agcggtgcct cttttgatcg gtacaggact
    27421 aggctttaat caagaacaga tgacttattt gatttcaatt gatatcttta tgtgtggatt
    27481 agcgacatta ttgcaattga cagttaaccg atttttcgga attggcttgc ctgtcgtttt
    27541 aggatgtgcg atccaagcag tagctccact gatcttgatc ggaaccaatg aaggagtagg
    27601 agctatctac ggatctatta tcgcttctgg gatctttgtt gtgttagttt ctggtgtctt
    27661 ttctaaaatt aaaaaactat tccctcctat agtaacagga acggttatca ccgttattgg
    27721 tttgactttg attcctgtag caatcgagaa aatgggtgga ggtaatgcaa cagcagctga
    27781 ttttggtgac aaaacaaatc tgctacttgc atttgtgacg attctcctaa ttgtaggcgt
    27841 gcaaatgctg gcaaaagggt tcattcgttc aatcgcggta ttgattggtt tagtcggagg
    27901 aagcatctta gctgctttca tgggaatgat tgatctgagc gcgattggag atgcgccgtt
    27961 gttccatgtt ccacaaccct tttattttgg taaaccaact ttcgacgtat ggtcgatttt
    28021 actaatgatc atcatttcca tggtcagcat ggtagaatca acaggtgtat attttgcact
    28081 tggtgacatt actgggaaaa atgttggaga agaagaattg aaaaagggat atcgtgcaga
    28141 aggacttgcc gttatcttag gcggtatttt caatacattc ccttatactg gcttttctca
    28201 aaatgtcgga ttggttcaac tttcgggaat caaaacaaga aaacccatct atttctctgc
    28261 attcttcttg attgttttag gattattccc taaaatcggt gcgttagcac aaatcatccc
    28321 tgagccggta cttggtggag gaatgttagt catgttcggg atggtggcag tccaaggaat
    28381 ccgtatgctg ttggaagttg atttcagcaa tgataaaaat ctattgattg ttgctgtttc
    28441 tgttggattt gggctgggtt tcaatgttac gccgacatta ttcagtcaaa tgccgcaaac
    28501 attgcaaatg ttcactggaa atggcatcgt aatgagtagc attacggcta tcatattgaa
    28561 tttgatcttt aatggattga aaaaagatga agaaatattg gaagaagcct tcaagaagta
    28621 atagagcaaa agaaaggttg tgaaatcagc gttttgacct gattgttaga gaaacaaacg
    28681 gaaaaatagt tcctcgtatt ttgccgtttg tttgagctaa taccgagggt cagctgattg
    28741 aacaccgttt atccataaaa aggaatgtga cctaactttt gtcacattcc tttttttcat
    28801 ctttaataaa cacgaacaat aaaatgtttt tctttgttaa cattcgaaat ttctttgaca
    28861 tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact gggaggaaaa acaatgacag
    28921 caaaaatctc tgtgattatg gggagtactt cagattggga aacaatgaaa gaagcatgtc
    28981 aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc
    29041 cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg
    29101 caggagcagg gggagccgcg catctgccgg gtatggtcgc atcaaaaaca actttgccgg
    29161 ttatcggtgt accggtccaa tcacgaacat taaatggttt ggattcgctt ctttcgattg
    29221 tccaaatgcc tggaggagta cctgtagcta ctacagcaat cggaaaagcg ggagcaatta
    29281 atgctggact acttgcgata cagatgcttt ccatgtatga tttagaattg gaggctaaat
    29341 tggctgaaag acgttcaatg ttggctaaaa ctgttataga aagtagtgat caacttggtt
    29401 aaacctttgt tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg
    29461 atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat
    29521 tgtccagccg cacaagttgc agattggcac attgaaagtg tctatgatga cacctttgcg
    29581 ctgaaagagc ttgccagaag aacagatgtc attacctatg aattcgaaaa cgtcagtgta
    29641 gaggcgttaa atgcgatttt acccatgtca tttattcctc aagggacaga cttgcttgcg
    29701 attacgcaag accgcctgtt agaaaaatct tttttggaaa cgaataacat cgtgattgct
    29761 ccttatgcaa cgatcgtcag tccgacggat atccaagatg cgattgacgg gatcggctat
    29821 ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca aggggcagta tgttttgaaa
    29881 agtcgggcgg atctagctcc tgccatggac cttttaagag aaggaacctg tgttttagaa
    29941 gcatggatcc cgtttgaaaa agagatttcg atcatggtgg caggaaatgg tcaaggagat
    30001 ttcacgactt ttccggtcgt ggaaaacatc caccacaaca atattcttca tgaaacgatt
    30061 gctccagccg cgattgatca ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt
    30121 gcagaagcag tgagcctttc aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc
    30181 gggctatatg tgaatgaatt agctcctcgt cctcacaatt ctggacatta ttctattgaa
    30241 gcttgttcta tgagtcagtt cgatgcccat attcgaggta tttgtggttg gccattagga
    30301 gaaaaagtac gtttgataag caatgcagta atggtcaatg ttttgggaga acaactgctg
    30361 gaaagctatc gcttgattga gaataagcca gactggcaat tccatttcta tggaaaaaaa
    30421 caagcaaaaa aaggtcgcaa gatgggacat atcactatct tgacagaaga catccagcaa
    30481 acgctggaag aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa catgttagaa
    30541 cgttatacac gccctgaaat ggggaatatc tggacggaag agaacaaata ccgtgcgtgg
    30601 ctcgaagtcg agattttagc agatgaagct tgggcagaac ttggtgaaat cccgcaagaa
    30661 gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg atcggatctt ggaaatcgaa
    30721 aaattaacac gccatgatgt cgttgcgttt acacgagcgg tttctgaaac attgggagaa
    30781 gaaagaaaat gggtccatta tggtctaaca agtacggatg tcgtagatac tgcttatggc
    30841 tatctcatca agcaagcaaa tgatatttta cgggaagact tgaaacgttt tacggaaatc
    30901 attggcgaaa aagcaaaaga acacaaatat acagtgatga tgggtcggac acacggtgtc
    30961 catgctgaac caactacttt tggattgaaa cttgccacat ggtattcaga aatgaaacgg
    31021 aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca
    31081 gtgggaacgt tcgcaaatat cccgccattt attgaagagt atgtatgtaa acaattgggg
    31141 atccgtccac aagaaatctc tactcaagtt ctccctcggg atctacatgc tgaatatttc
    31201 tcagcgatgg cgcttattgc tacaagtatt gaacgttttg caacagagat acgcggattg
    31261 caaaaatccg aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa aggttcttct
    31321 gctatgccgc acaaaaggaa tccgatcggt tcagaaaata tggctggtct agccagagtg
    31381 gtgcgcggac acatggtcac tgcctatgaa aatgtcacat tatggcatga aagagatatt
    31441 tctcattctt cagcagaacg aatcatcttg cctgatacca cgattttatt gaattacatg
    31501 ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg
    31561 aatatgtctg caacatttgg tttgatttat agccagcgcg ttatgctgaa attgatcgat
    31621 aagggaatga caagagaaga agcttatgat cttgttcagc caaaaactgc gtacgcttgg
    31681 gacaaccaag tgcagttccg cccgctgtta gaagaagatc caaccatcac atcggtatta
    31741 tctaaagaag aaatagacga tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg
    31801 atattcaaac gagtaggttt ggaataaaga agagtgagtt gcaaaattca attaaaatag
    31861 aatagcaaaa ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta
    31921 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
    31981 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
    32041 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
    32101 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
    32161 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
    32221 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
    32281 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
    32341 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
    32401 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
    32461 cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa
    32521 tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg
    32581 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
    32641 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
    32701 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
    32761 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
    32821 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
    32881 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
    32941 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
    33001 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
    33061 aacttgcaaa accggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
    33121 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
    33181 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
    33241 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
    33301 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
    33361 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
    33421 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
    33481 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
    33541 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
    33601 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
    33661 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
    33721 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
    33781 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
    33841 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
    33901 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
    33961 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
    34021 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
    34081 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
    34141 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
    34201 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
    34261 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
    34321 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
    34381 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
    34441 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
    34501 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
    34561 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
    34621 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
    34681 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
    34741 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
    34801 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
    34861 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
    34921 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
    34981 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
    35041 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
    35101 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
    35161 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
    35221 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
    35281 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
    35341 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
    35401 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
    35461 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
    35521 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
    35581 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
    35641 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
    35701 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
    35761 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
    35821 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
    35881 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
    35941 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
    36001 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
    36061 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
    36121 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
    36181 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
    36241 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
    36301 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
    36361 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
    36421 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
    36481 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
    36541 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
    36601 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
    36661 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
    36721 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
    36781 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
    36841 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
    36901 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
    36961 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
    37021 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
    37081 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
    37141 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
    37201 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
    37261 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
    37321 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
    37381 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
    37441 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
    37501 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
    37561 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
    37621 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
    37681 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
    37741 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
    37801 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
    37861 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
    37921 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
    37981 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
    38041 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
    38101 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
    38161 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
    38221 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
    38281 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
    38341 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
    38401 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
    38461 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
    38521 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
    38581 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
    38641 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
    38701 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
    38761 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
    38821 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
    38881 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
    38941 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
    39001 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
    39061 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
    39121 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
    39181 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
    39241 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
    39301 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
    39361 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
    39421 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
    39481 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
    39541 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
    39601 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
    39661 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
    39721 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
    39781 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
    39841 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
    39901 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
    39961 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
    40021 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
    40081 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
    40141 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
    40201 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
    40261 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
    40321 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
    40381 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
    40441 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
    40501 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
    40561 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
    40621 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
    40681 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
    40741 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt
    40801 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
    40861 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
    40921 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
    40981 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
    41041 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
    41101 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
    41161 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
    41221 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
    41281 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
    41341 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
    41401 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
    41461 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
    41521 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
    41581 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
    41641 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
    41701 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
    41761 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
    41821 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
    41881 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
    41941 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
    42001 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
    42061 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
    42121 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
    42181 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
    42241 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
    42301 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
    42361 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
    42421 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
    42481 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
    42541 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    42601 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    42661 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    42721 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    42781 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    42841 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    42901 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    42961 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    43021 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    43081 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    43141 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    43201 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    43261 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    43321 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    43381 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    43441 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    43501 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    43561 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    43621 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    43681 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    43741 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    43801 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    43861 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    43921 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    43981 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    44041 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    44101 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    44161 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    44221 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    44281 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    44341 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    44401 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    44461 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    44521 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    44581 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    44641 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    44701 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    44761 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    44821 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    44881 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    44941 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    45001 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    45061 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    45121 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    45181 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    45241 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    45301 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    45361 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgcg
    45421 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    45481 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    45541 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    45601 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    45661 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    45721 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    45781 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    45841 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    45901 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    45961 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    46021 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    46081 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    46141 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    46201 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    46261 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga
    46321 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    46381 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    46441 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    46501 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    46561 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    46621 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    46681 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    46741 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    46801 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    46861 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    46921 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    46981 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    47041 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    47101 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    47161 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    47221 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    47281 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    47341 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    47401 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    47461 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    47521 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    47581 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    47641 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    47701 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    47761 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    47821 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    47881 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    47941 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    48001 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    48061 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    48121 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    48181 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    48241 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    48301 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    48361 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    48421 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    48481 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    48541 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    48601 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    48661 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    48721 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    48781 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    48841 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    48901 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    48961 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    49021 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    49081 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    49141 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    49201 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    49261 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    49321 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    49381 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    49441 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    49501 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    49561 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    49621 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    49681 agcatattat tcgcgggatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    49741 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    49801 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    49861 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    49921 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    49981 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    50041 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    50101 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    50161 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    50221 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    50281 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    50341 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    50401 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    50461 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    50521 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    50581 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    50641 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    50701 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    50761 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    50821 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    50881 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    50941 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    51001 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    51061 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    51121 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    51181 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    51241 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    51301 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    51361 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    51421 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    51481 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    51541 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    51601 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    51661 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    51721 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    51781 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    51841 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    51901 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    51961 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    52021 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    52081 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    52141 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    52201 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    52261 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    52321 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    52381 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    52441 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    52501 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    52561 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    52621 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    52681 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    52741 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    52801 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    52861 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    52921 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    52981 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    53041 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    53101 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    53161 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    53221 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    53281 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    53341 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    53401 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    53461 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    53521 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    53581 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    53641 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    53701 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    53761 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    53821 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    53881 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    53941 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    54001 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    54061 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    54121 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    54181 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    54241 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    54301 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    54361 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    54421 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    54481 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    54541 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    54601 tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta
    54661 aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata
    54721 cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa
    54781 aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg
    54841 taagtttgat tagaaaatag aaaaaatgca gatgaaaaat aaaacgcttt ccttattgtg
    54901 attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat
    54961 ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa
    55021 atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg
    55081 agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct
    55141 agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt
    55201 taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat
    55261 ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg
    55321 cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga
    55381 taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt
    55441 acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga
    55501 agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg
    55561 tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga
    55621 tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc
    55681 aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca
    55741 aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt
    55801 cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat
    55861 cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact
    55921 atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa
    55981 agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa
    56041 agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga
    56101 caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca
    56161 atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa
    56221 aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat
    56281 gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga
    56341 ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc
    56401 gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc
    56461 tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat
    56521 cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt
    56581 catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt
    56641 cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga
    56701 agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa
    56761 gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca
    56821 gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat
    56881 cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat
    56941 gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca
    57001 caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt
    57061 tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa
    57121 gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg
    57181 gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa att
//
LOCUS       DAFCNJ010000014        57130 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000014 DAFCNJ010000000
VERSION     DAFCNJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.014"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..258)
                     /locus_tag="LNE35_001036"
     CDS             complement(<1..258)
                     /locus_tag="LNE35_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910209.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="LNE35_001037"
     CDS             complement(274..924)
                     /locus_tag="LNE35_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910210.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="LNE35_001038"
     CDS             complement(935..2107)
                     /locus_tag="LNE35_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBK6910211.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="LNE35_001039"
     CDS             complement(2126..2998)
                     /locus_tag="LNE35_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6910212.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="LNE35_001040"
     CDS             complement(3020..3940)
                     /locus_tag="LNE35_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910213.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="LNE35_001041"
     CDS             complement(3940..4209)
                     /locus_tag="LNE35_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910214.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="LNE35_001042"
     CDS             complement(4206..4586)
                     /locus_tag="LNE35_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBK6910215.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="LNE35_001043"
     CDS             complement(5053..6516)
                     /locus_tag="LNE35_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBK6910216.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="LNE35_001044"
     CDS             complement(6580..9597)
                     /locus_tag="LNE35_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6910217.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="LNE35_001045"
     CDS             complement(9748..10587)
                     /locus_tag="LNE35_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6910218.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="LNE35_001046"
     CDS             10732..10977
                     /locus_tag="LNE35_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910219.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="LNE35_001047"
     CDS             11040..11462
                     /locus_tag="LNE35_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910220.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="LNE35_001048"
     CDS             complement(11556..11873)
                     /locus_tag="LNE35_001048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910221.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="LNE35_001049"
     CDS             complement(11900..12106)
                     /locus_tag="LNE35_001049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910222.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="LNE35_001050"
     CDS             complement(12087..12269)
                     /locus_tag="LNE35_001050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910223.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="LNE35_001051"
     CDS             complement(12283..12633)
                     /locus_tag="LNE35_001051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910224.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="LNE35_001052"
     CDS             complement(12659..12961)
                     /locus_tag="LNE35_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910225.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="LNE35_001053"
     CDS             complement(12964..13197)
                     /locus_tag="LNE35_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910226.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="LNE35_001054"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="LNE35_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="LNE35_001055"
     CDS             complement(14628..15743)
                     /locus_tag="LNE35_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK6910227.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="LNE35_001056"
     CDS             complement(15749..16147)
                     /locus_tag="LNE35_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK6910228.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17176..17562)
                     /locus_tag="LNE35_001057"
     CDS             complement(17176..17562)
                     /locus_tag="LNE35_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBK6910229.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17559..18080)
                     /locus_tag="LNE35_001058"
     CDS             complement(17559..18080)
                     /locus_tag="LNE35_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK6910230.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="LNE35_001059"
     CDS             complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="LNE35_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK6910231.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19149..20564)
                     /locus_tag="LNE35_001060"
     CDS             complement(19149..20564)
                     /locus_tag="LNE35_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK6910232.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20533..22686)
                     /locus_tag="LNE35_001061"
     CDS             complement(20533..22686)
                     /locus_tag="LNE35_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HBK6910233.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            22944..23180
                     /locus_tag="LNE35_001062"
     CDS             22944..23180
                     /locus_tag="LNE35_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBK6910234.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            23199..23414
                     /locus_tag="LNE35_001063"
     CDS             23199..23414
                     /locus_tag="LNE35_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HBK6910235.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(24117..24869)
                     /locus_tag="LNE35_001064"
     CDS             complement(24117..24869)
                     /locus_tag="LNE35_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6910236.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(24884..26131)
                     /locus_tag="LNE35_001065"
     CDS             complement(24884..26131)
                     /locus_tag="LNE35_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6910237.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(26207..26353)
                     /locus_tag="LNE35_001066"
     CDS             complement(26207..26353)
                     /locus_tag="LNE35_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBK6910238.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(26457..26768)
                     /locus_tag="LNE35_001067"
     CDS             complement(26457..26768)
                     /locus_tag="LNE35_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6910239.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(26770..26967)
                     /locus_tag="LNE35_001068"
     CDS             complement(26770..26967)
                     /locus_tag="LNE35_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBK6910240.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(27361..29088)
                     /locus_tag="LNE35_001069"
     CDS             complement(27361..29088)
                     /locus_tag="LNE35_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBK6910241.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(29081..30274)
                     /locus_tag="LNE35_001070"
     CDS             complement(29081..30274)
                     /locus_tag="LNE35_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6910242.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(30461..31105)
                     /locus_tag="LNE35_001071"
     CDS             complement(30461..31105)
                     /locus_tag="LNE35_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6910243.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(31199..31333)
                     /locus_tag="LNE35_001072"
     CDS             complement(31199..31333)
                     /locus_tag="LNE35_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBK6910244.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(31335..33467)
                     /gene="feoB"
                     /locus_tag="LNE35_001073"
     CDS             complement(31335..33467)
                     /gene="feoB"
                     /locus_tag="LNE35_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBK6910245.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(33464..33937)
                     /locus_tag="LNE35_001074"
     CDS             complement(33464..33937)
                     /locus_tag="LNE35_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBK6910246.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            34338..34562
                     /gene="nrdH"
                     /locus_tag="LNE35_001075"
     CDS             34338..34562
                     /gene="nrdH"
                     /locus_tag="LNE35_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBK6910247.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            34721..36880
                     /gene="nrdE"
                     /locus_tag="LNE35_001076"
     CDS             34721..36880
                     /gene="nrdE"
                     /locus_tag="LNE35_001076"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBK6910248.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            36930..37895
                     /gene="nrdF"
                     /locus_tag="LNE35_001077"
     CDS             36930..37895
                     /gene="nrdF"
                     /locus_tag="LNE35_001077"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBK6910249.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(37984..39123)
                     /locus_tag="LNE35_001078"
     CDS             complement(37984..39123)
                     /locus_tag="LNE35_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6910250.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(39432..40145)
                     /locus_tag="LNE35_001079"
     CDS             complement(39432..40145)
                     /locus_tag="LNE35_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBK6910251.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(40399..41100)
                     /gene="nagB"
                     /locus_tag="LNE35_001080"
     CDS             complement(40399..41100)
                     /gene="nagB"
                     /locus_tag="LNE35_001080"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBK6910252.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            41334..42866
                     /locus_tag="LNE35_001081"
     CDS             41334..42866
                     /locus_tag="LNE35_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6910253.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(43057..44259)
                     /locus_tag="LNE35_001082"
     CDS             complement(43057..44259)
                     /locus_tag="LNE35_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6910254.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(44341..45030)
                     /locus_tag="LNE35_001083"
     CDS             complement(44341..45030)
                     /locus_tag="LNE35_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK6910255.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(45136..45504)
                     /gene="rplL"
                     /locus_tag="LNE35_001084"
     CDS             complement(45136..45504)
                     /gene="rplL"
                     /locus_tag="LNE35_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBK6910256.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(45557..46060)
                     /locus_tag="LNE35_001085"
     CDS             complement(45557..46060)
                     /locus_tag="LNE35_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBK6910257.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(46264..46391)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(46470..47159)
                     /gene="rplA"
                     /locus_tag="LNE35_001086"
     CDS             complement(46470..47159)
                     /gene="rplA"
                     /locus_tag="LNE35_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBK6910258.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(47263..47685)
                     /gene="rplK"
                     /locus_tag="LNE35_001087"
     CDS             complement(47263..47685)
                     /gene="rplK"
                     /locus_tag="LNE35_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBK6910259.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            48028..48696
                     /gene="sdaAB"
                     /locus_tag="LNE35_001088"
     CDS             48028..48696
                     /gene="sdaAB"
                     /locus_tag="LNE35_001088"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBK6910260.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            48709..49581
                     /gene="sdaAA"
                     /locus_tag="LNE35_001089"
     CDS             48709..49581
                     /gene="sdaAA"
                     /locus_tag="LNE35_001089"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBK6910261.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(49672..50691)
                     /locus_tag="LNE35_001090"
     CDS             complement(49672..50691)
                     /locus_tag="LNE35_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK6910262.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(50864..51769)
                     /locus_tag="LNE35_001091"
     CDS             complement(50864..51769)
                     /locus_tag="LNE35_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6910263.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(51828..52373)
                     /gene="nusG"
                     /locus_tag="LNE35_001092"
     CDS             complement(51828..52373)
                     /gene="nusG"
                     /locus_tag="LNE35_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBK6910264.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(52474..52644)
                     /gene="secE"
                     /locus_tag="LNE35_001093"
     CDS             complement(52474..52644)
                     /gene="secE"
                     /locus_tag="LNE35_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBK6910265.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(52668..52820)
                     /gene="rpmG"
                     /locus_tag="LNE35_001094"
     CDS             complement(52668..52820)
                     /gene="rpmG"
                     /locus_tag="LNE35_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK6910266.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            52982..53626
                     /locus_tag="LNE35_001095"
     CDS             52982..53626
                     /locus_tag="LNE35_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6910267.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(53682..54185)
                     /locus_tag="LNE35_001096"
     CDS             complement(53682..54185)
                     /locus_tag="LNE35_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK6910268.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(54198..54244)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            54782..55747
                     /locus_tag="LNE35_001097"
     CDS             54782..55747
                     /locus_tag="LNE35_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK6910269.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(55846..57018)
                     /locus_tag="LNE35_001098"
     CDS             complement(55846..57018)
                     /locus_tag="LNE35_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910270.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(57005..>57130)
                     /locus_tag="LNE35_001099"
     CDS             complement(57005..>57130)
                     /locus_tag="LNE35_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910271.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        17012 a        11485 c         9386 g        19247 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    16681 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
    16741 cgctgttctt tttttatttt tcaaaataac tagggctttt tatttttaac tcaaagtcca
    16801 ctgtagccct ttggactttg agttaaaaaa gaagcataat tatgaacagt aaagtttcta
    16861 atataacaat aattcgagaa gctttgcaaa atcatgctgc caatgaactc tctaaacata
    16921 taggattaaa tctaagtact ataaaaggtt aaaaagtgaa gaatatttaa tagaaaaact
    16981 aaatttactt gctgctttcc ccttttccat taatacaaca atcagagagt gtcttttttt
    17041 cgaaaaaatt agaataaata aaagacacgt agttttttta tctacatgtc ttccatcgac
    17101 tacaagttat catttctcaa aacgtattta gcttttaatt gattaatcgt ttcacgttgt
    17161 ctacacattg cctaattaag attttcttat tagaagcatc aatcccgcaa taatacctaa
    17221 aactccagga attacccaca ataatgttaa aagaaaaaat ggaataatag ataaaatgag
    17281 taaactaata ccatagacca ctgttttttg attttttctt actaaaaaac ctattatgaa
    17341 aatcattatc gaaaaaacta gggtaattcc tccaaataaa acaacattcc cgcttagatt
    17401 gactaacata tctatatcct tactggttag ttctgtagta tttaaagtag ttgaatcaat
    17461 agaagataga aacataccac ccatcaatgc taaaagacta cataaaatcc ctgttattcc
    17521 actaattaaa atcaaaataa actctgtttt tctcttattc atttaattct ctccagatta
    17581 agtgttttag aattagaaat tttttgacca tagttaatag cttctaatga acgaataaca
    17641 accactgacg tgttagaaaa ataatcccca atatgttgtt tcttgggcgt gaaagcagcc
    17701 accaaatacc caatttgaaa tacaaaattt tttaatataa acctacatat acactcacga
    17761 ataaagatag tttgccaact tagctctttt tcctcaaagc tcactactct tatcccaaaa
    17821 atcattttcc caattgtttg tcctgaattc agcttagtca aaagtacaaa ataggttaaa
    17881 taaatagcta atgacgctat accataaaaa gtaataatgg atttgtcaga ttccagtccc
    17941 atgattttgt aaaagagacc tatagtagca tgggtgatga tgctaataca aattaaatca
    18001 ataagaaaag caaaaaatcg taaccaaaat cccgcaaata cataattagg caaagtttca
    18061 atgccactct tcttattcat tttatagccc tccatagtaa tacattggtt ttggagtatc
    18121 agaaaatcca agtttttcaa aaactgaaat tacacgatct gatgtagttg cctttagccc
    18181 ttgccattca gcaatcttat tacctaacca agaagaagca aagccagttg tgtcattttt
    18241 atattctata accgtcgcat cagttaattt cttctctttt tttagggaat ctaatgcatc
    18301 ttcagagtaa ccaatttgat ccaccaaccc attttctact gcttggttac catcataaat
    18361 tcttccatcc gcaagttctt tcactgcatt agtatccata tttcttcctt ttgctacaac
    18421 attgacaaaa cgatcataag cactcatcac aaattcctgt ataactttat tctcttcctc
    18481 tgaaggtttg tgttgaggtg acatcatatc ttttaatgca ccacttttat acgtcgcatc
    18541 agttacgcca agtttttcta ataagccaga ataatttaaa ccagaaataa tgacaccaat
    18601 tgatccagtt gttgtttctt ccgtagcaaa tattttatct gaaccagcag aaatgtagta
    18661 ccctccactt gccgctgtat ttttaaatgc agtatagata ggaatatcta gctttttaat
    18721 ttttgccatt tctttagcaa tctcagcact ttcatatatt cctcccccag gtgaatttac
    18781 ttctaataac actcccttaa ccgctttgtc ttcttgaatt ttctttagct gtgttaaaaa
    18841 attctgatgg ttatattgtt ccctactaaa aagattgctt tcacctgtat ctgcaataac
    18901 tccattgacg gatagtttga caatcttttt atttgatgcg ccttcttcta acgtttcttc
    18961 tgtgatttcg ttagatccgt aaaaaatctt atttattcct gttagggttg tatttgatgc
    19021 ttcctcttta tcttttcctg gaattagcgc gacgataatg gatagtaaca ataatccgca
    19081 cgctactccc acagcaatcc atcttctttt attcatataa actcctccta tattcttagt
    19141 aaaaataatt attttttatt tagaaacaca tcgacaattt gttgccaata tgttttttct
    19201 cctacaatta aagacaactc tccagttaat ccataacgaa acggaaacga ttgagttgtt
    19261 tttaacgttc ctttcactgc ataaaaaaca cctttctcag tggcagtaga attttctgaa
    19321 atttcatcta aagttccgtc gatcgtaatc ggagccccac ctttttgatc caatttaaaa
    19381 tgaaccttca tcccttcttt aatataggta ctttcattcg caggtaaaag tgctgtaaat
    19441 ttcatttttg atttttcgtg gatcggataa atttctgcaa gcactgttcc tttggctagt
    19501 tctgcttgtc cctttgtttc ttcatttaaa tgaaccgttc cagatacagg agcttttagt
    19561 attccttttg aaagttgatc gtccaaactt ttgataactg actggttctt ctcttgtttt
    19621 tcatttagct ctgtaactgt ttgttttaca gttgttattg cttgttctag ctcttgcttt
    19681 cctttttccg tttgactaat tctgtcattc tcagaagtag ttggtttaac taatttagct
    19741 ttttctgact gatactgttc tacttctttt tttagttgct caatttgttc atttatgctt
    19801 gctgaaattg tcagtttcac ttgatttttt tgatcatcag aaacattatt tagttgttct
    19861 tgccaattct catagtttgc catcatttct tttgaaaaat cttttaatga ttgattatta
    19921 ctccaagcta cctgtacttg ttgccattct tgtaatttac tatctgaact ctcaatttgt
    19981 ttttctagtt gtctttttgt cttttcgtat acttctaatt gcttttgatc atttaattca
    20041 ctttgcttca aagcgtagcg gagtgattct ttttctgaca acatgctctt taattgatta
    20101 ctgtagccaa aactgtcatc cgttgaaaac aaattttttc ctttacttat actttcaata
    20161 aatagctgag ccatctttat ttgttgagta atacgttggt tttcttgttc tatctgagcc
    20221 ttttcattct ctattaaaga acaatccaat ctaaccaaca catcgtcctt atgaacaaat
    20281 tgattttctt ttaggaagtt ttccattatt ttagcttcta atggcacttg taacttctct
    20341 attttttcgg ggttcaattg agccgttgtc cgaataacaa cctctttttt agcaaaggtt
    20401 aaaaacaatc caataataca taaaaaaaga attacagggt ataacaccca acggtagaag
    20461 ctatgatgtt gtttagtata gatactagaa tgttcgaacc aattattatt ctgcataaat
    20521 tgtttccctc tcctagattt cccataattt ttgatagata ccatcggcaa aacgaagttc
    20581 atcgtgcgtt cctttttcta ccaaatgccc ttcatggaga actaaaattt gatcacatgc
    20641 ttttgctatt ggtaaatgat gcgcaataaa aataatcgtt ttttctttta aattgaggag
    20701 ataattcata attgcatgtt ctaacaaggt atctaatcca ctagttgctt catctaaaat
    20761 taatatgtcc ggatttttta ataaagcccg tgctaaggct aatcgttgtc gttgccctcc
    20821 tgaaagatta tttccacctt cttccaacaa tgtctcgtaa cgtaatggct gttgattaat
    20881 aaattcattt agttggacta tctcacaaac ttctattatt tgttcgaaac ttggtgggct
    20941 atctaaaccg aatataagat tctcaatgat tgttccacta aagaaaaatg actcttgagg
    21001 caaatatgtc actcgatcgc gtaatatctg aaaaggaatg tctaagtgat taatgtttcc
    21061 ataatggatc gatccttctg atgcttcata aaaacgaaca aataactttg caagagttga
    21121 ttttccagaa ccactaactc caactatagc cgttttactg tgcgaaggaa ttgtacatga
    21181 aatttcgttc aacgttgggg ccttcatgct atacgagaat gaaacgtttt ttacagtgat
    21241 atcttgttga aataattgtt cagaaagcat atattcaaaa gactggttcg atttttctga
    21301 atcaatcgcg aaaatttcat ttaaacgatt atttgcaact tgagccgttt gcattttcac
    21361 ttgtaaatta ataatgtttt gtaaaggatc tgtcaaaaat actagtaaag cattgtatgt
    21421 gataagttga cctagactaa ttctattatc cataacaaaa taagaaccta gccataaaat
    21481 taatgcactg ctaattaatt gaatcaaatg tttaattcct tgttgaatat tatcaagcgt
    21541 aaccgtacga aatgattttt tcattaattt aacaaattca tggtctatac ggttatatac
    21601 tttctcttca ccgctatatg ctttaattgt ttctattcct ttaagacttt ctatgatact
    21661 agaatttaca gctgcaccgg ctgccatttc ttccctgttc gatttttcat agcttttcac
    21721 gaaagcgaat attgcaacta cataaaaagg caaagagcat agagtgatta aaaacaacgt
    21781 actattctga atagctaacg taaccccaac aagcaacacc ataccaatat ctaaaaatat
    21841 tgataacgta gcacttgcta aagcatcaat aatcttactg gcatcaagaa aacgagaaat
    21901 aatttcccct gattttctcg tagcaaaaaa attcattggt aaagttaaca catgtttgaa
    21961 atactgaagc ataattgcca tactcattcg ctgtcctaaa ataataagaa gataactacg
    22021 actatattca aacaataccc gaaataaata gacagcgatc aagccaatgg aaattatatt
    22081 taaagttgaa cgtgcttgat tagggatgaa aaaatcaaga ataccttgaa aataatatga
    22141 gcttcctatg ccaaaaaacg taatgaaaat cgatgctaaa acgatgtgga atactaatcc
    22201 tttttgtttc caaattatag gcaaaaaaga acttaatcca gctacttttt ctttaatagg
    22261 aatataagtt tctctaggca taggtaataa aagaatacct gtccattctt tttgaaacgc
    22321 ttcaatcgtt tttctatttt ttccattagc gggatctgca atccatagta cgtttccctt
    22381 cgttttataa attacaacgt agtgcatata gctgttatca accaatatat gtgcaattaa
    22441 tggtaagata agatctcttt ctttccagat ctcactatct gcttgaatag ccatacaatc
    22501 aaaagacaat ttttcaaatg tctttttcaa tccaaatgct gaagtaccct ctaaatcagt
    22561 cccagatagg tcccgtaatt tatgaatcgg gatatcagtt ttaaaatatt ttaaaatcat
    22621 cgaaaggcaa gcaacaccac aatctttttc atcttgttgc tgcacaaaag gaaatgattt
    22681 tatcacttat ctaacacaac tccttctaaa aaatttttat agaaaaattc tcaatatagc
    22741 tttaatacag tgaaaatatt gtctcataat tccacaaaaa ctttgaaaac agttcttgaa
    22801 cactaacttt ttcattcctg aatcaaattt attttggaaa ttttactatt caagaataaa
    22861 cttgataagt ttcaggaaga tttttactgt taatcaaata cgtatgttac agtagcaccg
    22921 taaaaaatat tatttaaaga agcgtgttta aacatgcaaa atgtaaagga actaagtatt
    22981 gtggaaatgc acaaactgtt ggtggaggca ataatgcttg gggaaaattg gggcaagttg
    23041 taggtggact taccacaggg gcagtaggtg gtgcaggttt aggaacagca atttgcggac
    23101 cagcttgcgg tgttgttgga gggttatatg gagctgtcgc tggaggtgct gcagctggct
    23161 gggatgctcg aaaaaaataa aaaaggagaa taagtagtat gcatattaaa aacacaaaaa
    23221 caacatttat attatcttca gaagagttga aaaatattca aggtgggagt gccgtgggag
    23281 ttttaggcac tactttttct ggggcaacag caggagtaaa actttgttca gccggaggac
    23341 cttatgcaat agctgcttgt ggtgtaggtg gagctttatt aggtgctgga ttcagcatgt
    23401 ggaccggtgc gtaaacaatt aaaaaatatc aaacctcaag attggtttgt gtcgattacg
    23461 aacataggta tgttctttat cttttcaaaa gtttggaatg gacagaactt tttactaaag
    23521 cttcttgtgc tagtttttat aattatatta aatttttatc ttttattaaa gtcagataaa
    23581 aattctttgg aaatgtaatg gtagagatat ttttacaaaa ggacttgcag cgctgtagtt
    23641 ggtgctgcag gaggacctat ttcggccgtt acaggaggta ttggtaatgt acttgtcgga
    23701 tgtgcattaa agcctgttcc tgcaagttaa atttataaaa acaggaaagt aattatctca
    23761 aatcctatca agtacaatca taggattgca cttgacagga caaatttttt aactaaaaaa
    23821 tgaaaaacac tttctaaaaa ttagtagaat ttgccaaatc tttcgcatct tccaccatac
    23881 atttaatttt aattcccctt tatggtactt aataaaaaca agaaaagaac tattcatttg
    23941 gccctttttt ggttccagac catagaaata ttcctccagt aactaatatt gctgttatgc
    24001 acgtaactaa taacagctat aaaaattggt acaactttaa taggattcag aaaaatttgc
    24061 actagtgaag gaatctattt tattagaaaa atgcaatctc cctaaagaaa aaaccactaa
    24121 acagtgcttt ctttcaactt ctccttaatt ttttgtgctt tccctaaagt aaagtttctg
    24181 gccaactctg aatcaaaata tagtgaacgt tttttaaaat ctatttcttt aacgttaaag
    24241 atatttacta aacacgctcg actaattctt gttaacatag gatatttctt ttcaagctca
    24301 ctaattcttc cataaaattc gtactgccca tctttagtac acaatgataa tctatgtggt
    24361 aattttgaag cttctacaaa ataaatgtca tttatatcaa aagtaaatgt ttgagaacct
    24421 atggaaaaaa caaatgcttg attcgtaacc tgttttgttg catctatcct ttcttgcgct
    24481 aataacaata actcaacaat ctcgtttcga tactcatcta gtgtttgatc ttttgttaca
    24541 aatcctaacg tttcaactct tctttttatt gttacgggta acatctcatc atgagtagtt
    24601 gtaaaaatga tttttgcttg aacatcatat tttctgatta cttctgctaa ttctataccg
    24661 ttaacttcat gatttaaatc aatatctaag aaatatattc cttgctttgg tcggaattgt
    24721 ttgaggtatt ttttaacttc taacggactt tgtgttttca atactatctt aaatacttta
    24781 tcgtgaaaaa gaatgaaatt atcaataatt cgttctaact gatttaattg tataatttga
    24841 tcttcacata atattattgg ataactcata aatcctcctt ccctcaaaac aacaaaataa
    24901 cttgagtagt aaattgatac ggatcgtttt gatagttcac aaacatattg ggattctttt
    24961 tattgatttc ctctatacta gataaaccta acccttgatg tccctttttt gttgttattc
    25021 cttttttcat tagagtatct atagagagta gtatctctct acaactattc gatatcgaaa
    25081 tttctatctg tttatcatct cggtaaatag ctagagacag ttctctatct tgactctcta
    25141 tagatgcttc aattgcatta tcgagaataa ttccaagcac cctaatacag tcaaatattg
    25201 gaacaggaac ttcaataata gtacttggac attcgaattt acaacaaatc ttattgttat
    25261 ttgcttggta tagcttcgct atcaacaaac ttttcaaata ttcatttttt atatttttta
    25321 aataacgata ttcaaactta ttggttagat aagaattaga atagatctct aattctttta
    25381 tttctttttc caatatcgaa tcatggtttt gattagcgat ttcttttaaa ctcagcaata
    25441 aatttttata gtcatgtcta aatctggcaa atttttcttg atctttttcc agtgaatcag
    25501 tatatttttt tagataaacc aattcttttc ttttcaattg ctgttcatat tgttctcgtt
    25561 gcctaatagt tattctaata aataaaaata gaacaacaaa catttgaatg attaaaaata
    25621 ttaagacacc aaacacaaaa tgatcaaata cttgatattg atgagcaaca taagaaacaa
    25681 ataaaccaat tgtaagtaaa taaatcatga atatagcagt aagtttagaa ctggactgtt
    25741 caacaatcct atttatagct aattttctat ataaccaaat aaacatcagc aaaaacaaag
    25801 aatcaagcat tatctggaat actacataac catacccttt tatcccttca aatgaaaaca
    25861 tatacgtcat aaccatggaa gtaataatag atctgaaatc atcaataagc atacataata
    25921 gtaatttatt taaaagcact atattagttt tcttcttttc ctttaatata aaataacttc
    25981 cgattatagg tatagctgcg gaaacatcag taaataattc aacaaaaaaa gtgagtggta
    26041 tcaataaaca tacaaaaaga tattttcttt tgttaagatc agtattagtc atataaaata
    26101 agagaaaaaa gaaaaagttt cccacgaaca ttcttaatag ataagctttt gtatcgatag
    26161 taatcaagag atcccctcct gattttagga tatcataaaa aaaattttag ttacactttt
    26221 taaataatga aaaaacacat tccactaaat ttgatgccgg ttttccttga ggttttgttc
    26281 cagcacctcc tttaattttt ttaagctctt gatctgaaca ttgtttagca tttagtctat
    26341 ttttttcttc cattacgatt ccccctacaa actataaata cttttatgtc actgattcaa
    26401 tatacctatt tttctaatca aaaaattttg agacatattt aatattataa gtatcattaa
    26461 aattgagatt tatctccata atctgctcga tataaaccat ttattccagc agttcttcca
    26521 atttcactca aatttcttat taattcctct tgatattcag tgaatcttat tttattagta
    26581 taagcagtaa aatatagata attcaccaat ctatttatta aaggaccggg atctaattca
    26641 tatttttgtt tttctaattt taaatatacc ttttgtaaaa cctcaagaat atcagaatat
    26701 ttaggatctt tacttataga tatatcatta tataattcat gaacaatttg cttagcattt
    26761 tttttcattt tagcacttcc ctggaattgc tccacctaaa aaaccaccta tagacattcc
    26821 tgcaatacaa gtagttgcct tggcccaatc gaccgtacat ttatttttag tgcaatacac
    26881 tccatttcca taatattttc cactatgagt ggtaccccca tagataagtt gtgtctcttt
    26941 aatagacaaa atttttaaat gtttcataat ctaatcaccc ctaaattaat gtttttagac
    27001 tattattcta acattcatta aatttcaaaa aacaattcct tcttgaaacg tagcaaaata
    27061 attcctgaat aagaatttaa tcacttttaa gtatataaac tatgtatatt ctttaatatt
    27121 ttttgagaat gcacttcgta cataataaac tttaatcaca ttagtctttc atacctacga
    27181 tcgatctctt tgactcgcac ccataaattc ataatagttt gtcggcgtct ttggacgttt
    27241 cgaagatgag ctacgcattc tcttttttta tagacaaaaa agatagagac gaacatggag
    27301 cttccaaatt tggatatttg atccaatttt ggaagctcca tttatttcgt gtctgtcttt
    27361 ttattttttc cagctttcga ctaattcttg gttctctttg atccagtctt ttgctgcttg
    27421 ctgtggatct tttccattgt tgatttccaa cataactttt tccatatctt tttctgacca
    27481 atggaaattg tctaatactt tgtaagcttc tggctggtct tctttcaatc cttttcgaac
    27541 catcgtatgg atctgctctt cgcttcccat tccgttttca ggatcttcta agtatttcag
    27601 atcatatttg gcaaacatcc aatgaggtgt ccatcctgta actacaattg gttcgtgttt
    27661 tttgatcgct tgacctagtg caacggtcat tgctcctgaa gaagatgtct ctactttcca
    27721 gtctttcaag ttgtcatatt tttggatcgt gttttcagca gccgtaacta caccagctcc
    27781 tggctcgatt cctatgattt tcttacctgc ctgatcggtt aactcatcga tcgaatttac
    27841 atccatgtag gctggcactg ctaaaccaac ttttgctcct gtcaaattgg cacctaggtc
    27901 ttcgacttga tctttatatt gtgcatattg tgaaccatga gttttaggta gccaagctga
    27961 aaccatggca tctgactcgc catttgaaac agatttccac atgattgaat tatctaatgg
    28021 cgtcatcgtt acatcatagc ccatttgttt caatacttca ccgactacat ttgttgaagc
    28081 aacttctgta tcccattcaa cataagataa gttgatacgt tttgtttctt tggcagatga
    28141 aaaactccct ataccaatcg cacctaagat cactactact acagcaccaa tgatcagtcc
    28201 ttgacgtttt ttacttttct gtgttgttgc tcctgcagct tttttattgt ttggctggtt
    28261 caaatgctgt gtaaaacggt caatgatgat tgctaagata actaatgcaa ccccgttgac
    28321 aaatccattc ccgacttgtg ctctttgcaa tgctgataat acgccgcgtc ctaagccggg
    28381 tgcaccgatc atagaggcaa taacgaccat tgaaagtgac agcattgttg tttgattgat
    28441 ccctgccatg atcgtacttt tagctaaagg caattctagt ttgaatagtt tttgacgact
    28501 tgtgctcccg aatgaatcag aagcttctac taattcttta ggaacttgtc gaatccctaa
    28561 attcgtaaaa cgaacggttg gtggcagagc aaagatgact gaggcaaata cccctggaac
    28621 catcccgata ccgaaaaatg caactgctgg aatcaaataa acgaatcctg gcattgtctg
    28681 cataaagtca aggatcggtt tgataatact ttgggcttta ctgctttttg ccatcaagat
    28741 ccctaatggt accccaatga tgatcgagat cacactagat aaaagaacaa gtgtgacagt
    28801 attcatcaaa tcattccata gaccttggtt ataaataaac catagaccga ttaatgtgaa
    28861 caacgtaagt cctggacgtt tcttagaaat aaagaaagct gctaccgtca ataacaagat
    28921 aaacaaagga gctggaatca ctaacagtaa atttgtaatc cccgacatca ctgcctgtcc
    28981 aatcgtttgt aaaaatgaaa ataggccaga aaaagttgtc gtaaaccatt ctgtgatatt
    29041 ttctacccag tttgccactg gtaattgata gttcaataga ttattcatgt tcactcacct
    29101 cgcttgtttc tgctaatgct tcaatcacac tacctcggat gacgacacct tttaattttc
    29161 catcctctac aactgcaagt ggagtcggtg agtcataaat aatattgaaa atatcactta
    29221 ccaccatgtc tttcccaatc accgtcacat tttcatctag gaagtcgatc aatggacgtt
    29281 tttgcttcct agcttctaac gcttgatctg cagtgatgat ccctttcagg tgtctctttc
    29341 tgtcaacagc catcagcatg ctgacttctt cattacgcat ccttgtcaat gcaacgtttg
    29401 gtccatcaga ttcgatattc gtcgtcaatg caggtaccat gatgttttga gccgtaagca
    29461 ctttggaacg atcgacttct tccacaaatt cacgtacata atcatttgct ggattggtca
    29521 atatttcttc acctgttccg atctgcatga tttcgccgtc tttcatcaag gcgattctgt
    29581 ccccaatccg taaagcttca ttcaaatcat gggtaatgaa aataatcgtt ttttgaacat
    29641 tggcttgcag gtccagtaat tcgtcttgca tttctcgccg gatcagcgga tcaagagccg
    29701 agaacgcttc gtccatcagc aagatctcag gatcatttgc taaagctctg gctaggccaa
    29761 cccgttgttg catcccacca gataactgac taggatattg gtctttaaat gaaagcaaac
    29821 tagaattttc taatgctttt tctgcttttt cttgtcgttc ttctttaggt acaccacgga
    29881 cttctaaccc gtattctgta ttttccaaga tcgttcgatg tggaaacaga ccgaagtttt
    29941 ggaaaaccat attgatttta tgacgacgga cttctcgcaa ttcttctttg cttaacttcg
    30001 atacatcttc tccatcaata tagatactgc ccgaagttgg ctcaatcaga cgattcaata
    30061 gacggatcaa tgtggatttc ccactcccgg aaagccccat gatcacgaag atttctcctt
    30121 cttttacgtc aaaattcacg tcatacacac caaccgttgc accggttttc tttaaaatct
    30181 ccgttttcgg ctggtgatca ttcatcatat ctaaagcggc ttgtgtcttt ttaccaaata
    30241 tctttgttaa atgttttact tctactttta ccaaacaaat tctccttttt tatgtaaatt
    30301 catttttctt tctgaaaaaa gtgaccacac tatgttaaca tcatgtagtc actttgtcaa
    30361 aaatgaaaga aatttgcttg tccaaactga aaaaaacttg gctaaatctt tgtaaataaa
    30421 aggtttagcc aagttaatat ttttttaata tttcacacaa tcattctgca agctgatgct
    30481 ccgggtaaaa aaagtttgtc atacgctgta gtgtatattc atttccaata aaatgaacga
    30541 tgtcccctgc cgtaaattta gcataggggc caggtgaaac aagcagttct tcattatgag
    30601 aaattgcgat aactgtggcg ccagtcgcct gccagatatt caactcgcta attgaacggt
    30661 ccagatgcat tgctttttct gataaagata attcaaacgg aagtaaagga ttcatcttat
    30721 gtattcgctt cgtttgattg accagctgat ccaataattg agaaaaatga ttcagctcat
    30781 cttgttgctt ctccacactt gccatgattt ctgcttttgt atcttggatg gattgtacat
    30841 cttgatattg caataggaaa tcttttgctt tttccttcga agcaacaaat gccccacttc
    30901 catgccgtac ttccatgatt ccaagatcta ccagcacatt gatagccttg cgggtagtct
    30961 caggagaagc attgtacgta ctggcaagtg ttgagcgggc atggatcttc tcacccactt
    31021 tatatcggtt ttcaacgata cgttcggcta aatcaacagc aatctgctgg tagcgcgggc
    31081 ggacgatttt tttctttcca gacattttct ttcctctttc tttttttact ttccctcatc
    31141 ttactatgct ctagcaaaat aagcaaaaaa ggggagaata gcctcttgca acgactgatc
    31201 atcgcttgat ctgttccttt tttactgggc aagctgtttg acagtcatca caattcttac
    31261 cggattttaa acgagtatag acaattgcgc ctgcacttcc aaagattaac acacttaata
    31321 agaatgtcgc catcctattc ctctccttct aatgacaata tagtataaaa ctggttcttg
    31381 gattttggct gacggaacaa aacataaacg agtcctatca tcagtaaaat agcaagatac
    31441 gtccctaacg ctacctgtcc accctccaat aagtgtccca actgataaat cacaaaacta
    31501 actaaataag ctaaaccgca ttgatagcct atggcgatcc aagtccattt cttggcattc
    31561 atttcccgat ggatagcccc aattgccgca aaacaaggcg cacaaagtaa attgaaggct
    31621 aacaatgaat aagctgctgc gggagtatat gtgtgttgta gcacttgcca gatttctctg
    31681 ccattttcgg atacttccgc atgaccaaac aaaataccaa atgtgccaat aatattttct
    31741 ttagcgacca atcctgtaat cgctgcgact gttccttgcc agtttcccca accaagcggc
    31801 tgaaaaacaa ctgccaggat tcgcccgaaa aaggctaaga tactttggtc ttcagaaaca
    31861 ggttgagcat aaaaattata agaagagctg aaccagatca cgatactaga aacaaagata
    31921 atcgttcctg cttttttaac aaaagacttg ctgcggtcat aggtttgtct taggaccgct
    31981 gaccattgag gaaaatgata tgcgggcaat tccatgataa aaggagctgt atctccagca
    32041 aacaaacgtg tttttttcaa gctgattcca gagaaaataa ttgcagtcat accaagaaaa
    32101 taggcagaag gagctaccca agattgatga ggaaaaaagg cccctgctac taaggcaata
    32161 atcggcaact ttgcagaaca aggcatgaag gttgtgacca tgatcgtcaa acgacgatct
    32221 tgctcgtttt caatcgtgcg actggccatg acgcctggca ctccacatcc tgtagcaatc
    32281 agcataggaa tgaaggattt tcctgaaaga ccgaatttcc tgaaaagtcg atccatgaca
    32341 aaagcgatcc tcgccatata tccgcagtct tccagaaagc tcaagcataa aaacaatata
    32401 atcaattgcg gtagaaatcc taacactgct cccacgcctg cgatgatgcc gttcaagatc
    32461 agctcctgca tccatcccgc tacttgccat tcagctaaca atctgccaac atgttctggt
    32521 aaccatgtac caaacaatgt gtcattgatc caatctgtcc ccattgttcc tactgtttga
    32581 atagccagat aatagatcag ccacatcaca aatgcgaaga tcggcaaggc taaccaacga
    32641 ttcgttgcaa tctgatcgat tttgtcactc atcgataatt ggaaagaatc atttgacaca
    32701 acacagagtg tacatagttg ggtaataaat tcataacgtt cattgaccag aatcgtctca
    32761 ctatcgtcgt ccaatagttt ctctgttaac tggatcagtt gctcgatctc tttttgctga
    32821 gaaactgcta tctcgtaatc ctctcttacc ttctgatctc gttcaaaata tttcattgca
    32881 taccagcgtt tcttgccgct ttgttctcct agtacatctt caatttcatg taaagctgct
    32941 tctaaacgtg gatcatatgt acagggaaca ggaatagaag gccgcttttt tgcagactcc
    33001 tgaatctttt cgattagttt ttcgattcct tgctttttca atacgctgat tggtaaaact
    33061 ggtacaccaa gaccatagga aagcttttct agattgattt tttttccttc ttttttgatg
    33121 acatccatca tattcagacc aatgatcata ggaatgtcta aatccaataa ctgcgtagtt
    33181 aaataaagat ttcgttctaa attggtagca tcaataatgt tgatgatcag ttcaggctgt
    33241 tcctgaacca aataatctct tgcaatcact tcttctggtg tataaggaga caaagaatag
    33301 atgccgggta ggtcctgaat atgaatgttc ggattttttt tcaacgatcc ttcttttttt
    33361 tctactgtta cgcccggcca attccctaca tattgattcg aaccagtcaa gagattgaac
    33421 aagctagttt tcccgctgtt tggatttccg actaacgcga tattcattct ttttcctccc
    33481 tttgtatcaa gattagttcg gcctctgact ttcgtaacgt cagttcatac cctcgcactg
    33541 tcagttcgat tggatctccc agaggggcta gttttcggat aactattttt acatttcgcg
    33601 tcaatcccat atccattaac cgtcgcttca ctgcgccttg tccgtggatt gctacgacga
    33661 tcccagtctc tcctgctttt aattgatcaa gagaagtcca gctttcagag gaagcagtct
    33721 ttttattgac aaagatttga cctagtattt cccgacttac tgccactcga tgacttttta
    33781 ccagtaaaat ggcattgttg cctgataaat tgacgacgac gattttactt ccggcaacga
    33841 tcccgatatt ttgcaaatgg cgttttgttt tttcttctgt ttcgattcgt tcgacttgat
    33901 aaacgaaatt ctgctttgcc tgcgctaagg tctgcatatt gattcctctc ttgtgaaata
    33961 aatcatgtat taaaatcatt cattatatgt tctaattttc ttacgtgtta cgcaaataaa
    34021 tgagaatcat tctcacatag aatactctcg gtaattgaga atgtcaatta tacttgtgat
    34081 attatgagcg aatctttagt attgatagat aaaaatgaga aaaaaatatt tcttcatagt
    34141 tttccacaat tttttctcta ttaaatttgc tattttccca cacgatactt gttttcagct
    34201 cttttttcaa aaacacaaaa cttagtattc cacagtttcc acaacacaat atgtagtgtt
    34261 tgttgtttga cttttttact aagagtttta tactatctat tatagataat aagaatgtgt
    34321 tagaggaggc actcaccatg aacgtaaaaa tcttttctaa aaataattgt atgcaatgta
    34381 agatggtcaa acggttttta acagaaaatc aaattgcttt tgaagaagtc aatatcgatg
    34441 aacagcctga agcagtagac tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta
    34501 cttctgatgc agaaacagtt atcggattcc gtcctgatca gttgcgtaaa cttgccagct
    34561 aacatgacta aaagaatcaa aacgtgatac atggatttga agggcttgag acagcaccag
    34621 tttttgtcgc aaacttccaa atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa
    34681 tcaagcatca tcaaatacga aatgaaggtt gggcgtacgt atgagtctta aaaatttaca
    34741 agatgtcagt tatttcaagc tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc
    34801 tttgaataag gataaagaag ctttagttgc cttttttgag gaaaatgtca aaccaaatac
    34861 gatgactttt ctatctatta tggaaaaaat caattacttg atcgatcaag aatatataga
    34921 agcagaattc attcaattgt attcaccatc atttattgaa aagctatatc aatttttgga
    34981 cgagcaacat ttttctttca aatcttttat ggctgcttac aaattttatt ctcaatatgc
    35041 gttgaaaacc aacgacggtt ccgcttatct ggaaacttat gaagatcgag tagccttcaa
    35101 tgcgctttac tttgcgaacg gggatgaaga attagcactt gctcttgcag acgaaatgat
    35161 ccatcaacga tatcagccag ctacaccttc tttcttgaat gctggacgta aacgtcgggg
    35221 cgaattggtg tcttgtttct tgactcaagt cactgacgat atgaactcta tcgggcgctc
    35281 aatcaactct gctttgcaat tgtcccgtat tggtggcggc gtagggatta ctttatcaaa
    35341 tcttcgtgaa gccggcgctc ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc
    35401 agtcatgaaa ttattcgaag atagtttcag ctattctaat caattaggtc agcgtcaagg
    35461 agctggcgtg gtttatttga acgttttcca tccagacatt attgctttcc tttcagcaaa
    35521 aaaagaaaat gcagatgaaa agattcgcgt aaaaactctt tctcttggcg tgatcgtacc
    35581 tgataaattt tatgaattga cacgtaaaaa cgaagatatg tatttattta gtccatatag
    35641 tgtcgaaaaa gaatatggtg tcccattctc ttatgtaaat attacagctg agtatgatca
    35701 tctagttgca aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga
    35761 aatctcaaaa ttacaacaag aatctggcta tccttacgta atcaatattg atacagcgaa
    35821 ccgcgagaat ccaatcgatg gcaagatcat catgagtaat ttatgttcag agattttaca
    35881 agttcaaacg ccttctgtga ttaacggcaa acaggaatat gaagtattag gaacggatat
    35941 ctcatgtaac ttaggctcga caaatatcgt gaatttgatg caaagcccta actttggtca
    36001 atctgtgcgt acgatgactc gtgcattgac atttattaca gatgcatctg atatcgatgt
    36061 cgtaccaccg atccaaaacg gaaataagct gaatcatacg attggattag gtgccatggg
    36121 cttacacact tatcttgcaa aagaacaaat ggaatatggc tcagaggatt cattagactt
    36181 tacaaatatt tacttcatgc tgttgaatta ttggacactt gttgaaagca acaatatcgc
    36241 tcgtgaaaga aaacaagttt tccataattt tgaaaaatca gcttatgctg acggcactta
    36301 tttcgaaaaa tacgtaactg gagaattcca gccaaaatca gaaaaagtca aagcattgtt
    36361 tgaaggcatt tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa
    36421 agatggactg tatcatcaaa atcgcttggc agtagcacca aatggctcaa tttcatatat
    36481 caacgatacg agtgcaagta ttcatcctat cactcgcatg atcgaagaac gccaagagaa
    36541 gaaaatcggt aaaatttatt acccagcgcc ttatctatcc aatgagacga ttccgtatta
    36601 cacttctgct tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa tggcacaaca
    36661 acacgtcgat caaggcatga gtttgacttt attcatgcgt tctgaaatcc cagaaggcat
    36721 ttatgaatgg aagacaacaa cgaagcaaac gacacgtgat ttgaatattt taagacatta
    36781 cgctttccac aaaggaatca aatccattta ttacgtacgt acctttactg atgatgcaga
    36841 agaaatcgga agcaatcaat gtgaaagctg cgtgatctaa tatcatagat agattcgctt
    36901 attcatattt taattaaagg agcattctca tgtcagaaac atattatgaa gcaataaact
    36961 ggaatgaaat tgaagatatc attgataaat ccacttggga gaagctgaca gagcaatttt
    37021 ggctagatac acgtatccca ctttcaaatg acctagatga ttggcgtaca ttgtctcaat
    37081 tggaaaaaga tacagtagga catgtatttg gcggtttgac actgctagat actgtccaat
    37141 ctgaaagtgg catggatcag ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt
    37201 tgaacaatat tcaattcatg gaatccgttc atgccaaaag ttattcttct atttttagta
    37261 cactgaatac gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg aatccttatc
    37321 tgcaaaaaaa agctgagcgt atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga
    37381 aaattgccag cgtcttttta gaaacattct tgttctattc aggtttttat actcctcttt
    37441 attatttagg aaacaacaaa ttggctaacg tagcagagat catcaaacta atcatccgag
    37501 atgaatctgt acatggaacc tacatcggtt acaaattcca attaggattc aacgagttac
    37561 ctgaagcaga acaagaaacg ttaaaagact ggatgtacaa tcttctttat gagctttacg
    37621 aaaacgaaga acgttataca gaagaattat acgatccaat cggctggaca gaagaagtca
    37681 agacattcct acgctataat gccaacaaag cattgatgaa cctagggatg gatccactct
    37741 ttccagatac tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa
    37801 gcaaccatga cttcttctca caagtcggaa acggctattt gttaggtcat gtagaagcga
    37861 tgaaagacga cgattatttg attggattgg aataaggatt tattttaatt caaaaatttt
    37921 tcgaaaaata aaaagagttt agcgtacaga tattttaaat gtacactgaa ctctttttta
    37981 tgcttattca cttgtttctt ccagcacttt tggactgtct aacttttccg aaacatgttc
    38041 cattcttctc aataaataga ttttccggat atttaatatt atcgtcttaa tcactgcata
    38101 gacaggtatg caaatcagca tgcctaatat tcctgcgaca cttcctgctc caatcaatac
    38161 caagatgatc gtcaaaggat ggatattcat tgatttccca aataacaacg gcttgatcaa
    38221 atttccatct aattgttgga tcactaaaat cgacaacgcc atatacaaag cttgaaatgg
    38281 agagataaac aaaccgacaa tgattgctgg taccgctcca ataaaagggc cgacatacgg
    38341 gataatattc gtgattccgc aaatgaccgc aagcaacaag ctataaggct gatcgaatat
    38401 tctcattgcc aaaaagctca ttacaccaat aatcaaagca tctaagatcg tactgctgat
    38461 ataggcagat aatgtttcgt ttaattcttt taccgtttgg cgtaactctt tacgaatcgc
    38521 tttcggaaag aagtgagaca acgcatcaat aaatttgtgc ccatccttga acataaatag
    38581 caaaataaat ggaacggtaa ataacaaaat aaaaaattgc atcataaatg aaatgatttt
    38641 tgagacactt gcagataaac tgactaatat gatattgaac aaattactaa gagataaatt
    38701 tgctgatgct aattgttctt ccaaattgaa atttttaaac tcttcccgct gcgccaattc
    38761 atttaaccat ttgcttgttt catctgcata cattggcaag ctctgggtca attgaattgt
    38821 ctgttccatt aactgaggca ctacattcat gacagttaaa acaactaaaa tgattacgac
    38881 caaaaacgtc aataaaaaac cgaacactct cgggatccga tgcttttcga gaaaaacaac
    38941 gattggatcg aacatataat agataaaacc agcaacaagc aatggtaaaa aaactgccga
    39001 aagcattttc aatattggac taaaaatatt cggcatttgt aaaataaagt acacgccgat
    39061 gatcgtgagt actagctcca ctgtccagaa aaataatttt gattgtttaa aacgtgattg
    39121 catacatctc ctccaaggtc ctttgatact tctattctat catttaacaa agagttacgc
    39181 gctgagattt tcatttgctt aaaaaaaaca aaaaaaataa acatatttta atgagtagac
    39241 gagtacttaa acaaaaacac ggtttggagt agtgacaaaa attatactgc agtccctttt
    39301 tccaaataaa cggtgttcaa aagcttgcat cttcgtaata agcccagcca aacgaaaaat
    39361 ataaggaact atttttattt ggctgggctt attacttgat gtaggacatc tattttacaa
    39421 cctcagatca tttattgcgc aatactgact aaatgcaact ttgtgatatt tttgtgtacg
    39481 gacaactcct cgatcacatc ggcataagtg agcccttttg gaagatcaat cgtatagata
    39541 tttttgtaca ctcggtagtc atcaacgaat tttacgtcaa aattcacatc ttcgattacg
    39601 atatttttct cttcaaaata atcggtaaga aaatctttgg tttcttttct atggataaat
    39661 tggatttcta actttttagt cgtaggtaca tggatcacgc gtttcactac tgtgagtgca
    39721 atagcaatcc cgataaaact gatgatcgca atggaatagt aacccatgcc gatggcgatc
    39781 cctaatccag caattgccca taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc
    39841 gtgacgatga tcgttcccgc gcctaagaaa ccgacaccac tcaccacttg agcaatcagc
    39901 cttgcttcat ctgaacgaat cgttccagca agctgtggat actctaaagc atctcttaat
    39961 gcaccactag cgatctcaac ttggatcaat gcaatgatac atgctcccat acaaactaaa
    40021 atatgcgtac gcatacctgc tggacgattt ttatattgtc gttcaaaacc gattgctccg
    40081 ccaaatagca tagcgatccc taaacgtaaa ataatctctg gaattgtcaa taccatatca
    40141 ttcatctaaa caccctccag tccaatatat catgtctgaa aggagcagac aaaaaaagtg
    40201 caatttttac ctcttacgaa aaaaggactg tgacaaaatt tttgtcatag tcctttttat
    40261 gaataaacgg tgttcaatca gctgaccctc ggtattactc aacaaacggc aaaatatggg
    40321 gagctatttt tcctttgtct tctctaatac tcaggtcaaa acgctgattt cacaacctct
    40381 ttagcctact attgaaaatt acaacttact tgctgcttct tcatcgatga tcacaacaac
    40441 attgtcgtga ttttgcaata cacttgctgg caaatgattc gttacaggtc cttcaatcat
    40501 ccctttgatt gcatcagctt tgctttcgcc aaatgccatc aagacgattt ctttactttt
    40561 cataattgaa ccgattccca ttgaaacagc ttttgttggt acatcttctg ctttatcgaa
    40621 gaatcgtttg tttgcattga ttgttgattc tgttaagtca actactgaag tcgttccgtc
    40681 aaatgaagca ccaggttcat tgaaaccgat atgtccattt tgcccgattc ctaagatttg
    40741 gatatcgatt ggatgtgcat cgatgatttg atcatacttt ttgcatgctg cttccagatc
    40801 ttctgctttt ccatctggta caaacgtttc tttgaatggt tttttatcaa ataattggac
    40861 atccatgaaa tgacggtagc tttgttcatc tgaaccgcct agtccaacgt actcatctaa
    40921 gtttacagaa gtcatttcac tgaaatccaa atcgcttgaa gtcatttctt tatataaagt
    40981 aactggtgtg cttcctgttg ccaatcctaa tacttttgca cctttgttca taccttcttt
    41041 gatgatttca aatgcttttt ttccgccttc ttcagcgttt tttacacgaa tgatttccat
    41101 agtgattgct cctttatatt ttggtatagc ccaattatag cattcttttt ttctttttac
    41161 aataattagt atagaccaaa ttgcattttt ttcattttct tgacaataaa taggaatccg
    41221 tgttaggttt cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt
    41281 gattcactgt ggacgcttta tgtctgcagt ttttttatga aaaaggagtt attatgagta
    41341 tactgacatt aaacaacatt acccagcaat ttggcgacaa aatcctctat gaaggtgtcg
    41401 atctgcaatt aaatgctggc gaacatcttg ggctgattgg acaaaacgga gccggtaaaa
    41461 gtacattgat taaaatcatc actggtgaga tcttaccaga tgacggacag atccattggc
    41521 aaaaaaatat tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa acattaacaa
    41581 tcgaagaatt tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt
    41641 ttctttacca aacatatagc gagaccttcg atgattcctt gctggaaaaa gctgggaaac
    41701 tccaaacgga attagaccaa ggagtgtttt atcaaatcga caccttagtt gctgagatga
    41761 gcagcgggtt ggggatcgat gtcttaggtt tagattcact gttgaaaaat ctaagtggtg
    41821 gacaacgttc aaaggtgatt ttagcaaaac tacttttaga aaacccagat gttctgatct
    41881 tagatgaacc aaccaatcac ttagatgatc agcatatcca atggctgacg cagtttcttc
    41941 aggattttga cggtgcgtat atcgtgattt cccatgacca agaatttctt gatcagatca
    42001 ccacccatat cgcagacatc gagtttggaa agattactaa gtatactggc catctaaaac
    42061 aagcattgaa acaaaaggaa caaaatcgcg aaagctattt gcgccaatat catgctcaac
    42121 aaaaacaaat cgaaaagacc gaagcttata ttcgaaaata caaagccggt tcccgatcaa
    42181 cgatggcaaa aagccgacaa aaacaattag ataaaatcga acggctgacg cctccagctt
    42241 cagcaagtaa agctgtcttt gattttccat tctcgcctat tgtcacgaca ttagcagtgg
    42301 aaacaactga tttagtcatc ggatatgatc gaccgctact tgcacccatc aatttgacca
    42361 tccgctttgg tgaaaatatt gccattcgag gcttcaacgg gattggaaaa tctactttat
    42421 taaaaacact gatcggtgag attgaaaaaa taagtggcga tttccacttc ccagataaca
    42481 caaaaatcaa ttacttttca caagatctgt actgggaaaa tccattagaa acaccactac
    42541 aatatttaag cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc caactagcta
    42601 aagcagggtt agttaatcaa ttagcaagtg aacctctcac tacattaagt ggtggggaac
    42661 aaactaaggt gaaacttgca cagttgacaa tgaaccaagg taatttactg attctcgatg
    42721 aaccgacgaa ccatatcgat caagaaacga aagaaagctt acaagacggg atacagaaat
    42781 ttccaggaac agtaatcatc gtctctcatg aacaagaatt ttatcaagat cttgtgcaac
    42841 gagtcattga gattgaacca aaataaacgt ttgattgaat agttgaaaaa aaggagctgt
    42901 gacaaaagtt ttgtcacaac tccttcttct gaataaatgg tggccaatta gctgaccttc
    42961 ggtattagct caaaaaacgg caaaatatga ggagctattt ttatttggct attcttatta
    43021 cttgatgcag aacagctttt cacaacctct tttcgtctat tttttatttt cgagatttct
    43081 ctttccttta aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct
    43141 aaccgttaca cctaaccagc catactgctg ccacattgtc gtccctagca atgaaccagc
    43201 cgcacctcca ataaagtaca taaacataaa tacagtatta tttcggttgc ttgctgcttc
    43261 acctagccct tgtactcgtg tttgattggc gacttgacca aattgattcc cgacgtctaa
    43321 taaaataata gctaaaagca aaatggccaa atggcttccg ccaaccaata aagcaataaa
    43381 gcttattgtt tgcataacaa gtcccatata cacgattttt ctttcagaat aagaatctgc
    43441 cagtcgtccc acgataggtg cagcaactgc tccagacaaa ccaaaaatcg ctaatatccc
    43501 tgcttcgaat gttccccaat tgtaggcggg actgctgata taaaaaatca acgtagacca
    43561 aaagatcgaa aatgttccaa acatgaaaaa tccactgact gccgcttctc ttaatagacg
    43621 ttgagaagca actaaatgcg gcaaactttt aagtgaatca aggtatgaaa cagctgtact
    43681 actttgctgg acagaacgcg gcattttcca gtaaagcaga agtgtaagaa ttccgactgc
    43741 acataaagca aataaataga tcatccgcca agaaaaaaga ctggcaacaa taccagatac
    43801 cgtccgagaa agcaaaatcc ccacgagtaa accgctaagc aatgtaccca tgaccttccc
    43861 tcttgctttt ggtcccgcta gtaccgcacc gtaaggaatg atgatttgcg gcacgatcga
    43921 aagtaatcct attaaaaaag aagcgcatgc aaataacgta aacgaaggcg caaaaaaagc
    43981 ggccaacaga gagacggcag ataacgctgc cattcgaatg atcaattttg gacgatccac
    44041 cacgtcccct aaaggaacta aaaacagtaa ccctaacgca tagcctaatt gggtcaacat
    44101 cgtcaatatc ccgactgcac ttgtgctaac agataaacta gctgctactt ttgttccaat
    44161 cggctggatg taatacatat tggcaacaac aacgccacaa gtgattgata atagtaaaat
    44221 aagcgaagtt gttaaaggtt ttttttcatt actcttcatt gatttccccc atttcctatc
    44281 ttagtttaca acttatggaa aagattggaa atagaaaaat acaactattc gccacactta
    44341 ttatttgtgc ttttgttttc cttgtttttt cttgcgatct ttataaatga agacaactaa
    44401 ccctactagc acagtaacac ttgcaaccaa cgtgactgca gttttgttat atccgtgaaa
    44461 cagagcttct ccaccacacg catacaacac agacgaaggg atgactccaa tcacgatcga
    44521 caatatgatt tgctgtagag gcaatttcat cgcatctgca gcaaaattga ccatagaaga
    44581 tggaataacc ggtatcatat agcctaacat tacacctatt tttggatgtt tcatttgtcg
    44641 gattgcttgc acccaatgat tcgtttcggt tttcttatct aaaaatttta agcgatgcat
    44701 aaggaaaatt gcagtgatgt taccaagagc attacccaaa atgttgatga tcgcacctgt
    44761 cagtggtcca aagctcaaac cagcaatgac tccaactact gaagtaggaa tacctggaac
    44821 agcacacatc accgctgtca atacgatcaa gagtatcgat gcaaaagtcc catgcgaacg
    44881 aatatgtgcg agtagctctg ccttactcgc tctcggattt aaaaataatt ggatgtctgt
    44941 ctgatattct aaatacaacc gatagcccac taccagtatc agaatgattc ccaacgagat
    45001 caatatcaat cggattattt tctttttcat atttcctcct gaaaaaaatc tatattcatt
    45061 aaataatagt ttaacataaa aacaaaaaag caccgaaatc caacatagga tctcagtgct
    45121 tttaaatagt aaaaattatt taacagttac agatgcgcca acttcttcaa gtttagcttt
    45181 caattcttca gcttcttctt tagatacgcc ttctttaact ggtgcaggag cgccatcaac
    45241 tacagcttta gcttctttca agcctaaacc agttgcttca cgaactgctt tgataacttt
    45301 aactttttgg tctccagctg cagttaattc tacagtgaat tctgtttgtt cttcagcagc
    45361 agcagcgcca ccagcagcag ctacagctac aggagcagca gcagatacgc caaattcttc
    45421 ttcaatagct ttaactaggt cgttaagttc taggatagtt gcttctttta actcagcaac
    45481 gatgttttca atgttcaatg ccattataat ttcctccgtt taattgttct tttttattta
    45541 atgctttatg cggcaattaa gctgcatctc catcttcttt gtctgccact gctttgacag
    45601 cgtaagccac gttgcggact ggcgcttgta gtacagatag caacatagat agaagtcctt
    45661 cgcgatttgg caattttgcc aatgctgtga tttcttccaa tgaagagact ttaccttcga
    45721 tgataccgcc tttgatttct aatgcttttg cttctttagc aaattcgtcc atgattttcg
    45781 ctggtgcgac tacatcttcg ttactgaatg caacagctgt aggtccagtg aatacttcgt
    45841 ccatgccttc aaggccagct tttttagctg cacgtgaaag aatagaattt ttgataactt
    45901 tcatttctac gttagcatca cgaagttgtt tacgtaaacg tgttacttca tcaactgtta
    45961 atccgcggta gtcaacgact actactgatg cagctgctgt aaatttttcg cttacttcat
    46021 caacgattgc tgcttttttt gcgataattg cttcactcat ttttgagttt cacctcctga
    46081 attttgatgg aagagttttt tgcactgaaa aggcaagtca cttttatagt tactttgctc
    46141 aaccagtatt attcatcacc tattcacttc gttcatagtg attctactcg ctttgctcga
    46201 aaggaaactc ctttcgcttt gctcaaccag tattgttcat catctcttca cttcgttcat
    46261 agtgattcac aataaaaaac tctatgccac cgcagacata gagggactat tgaatatatt
    46321 caataaacgt cctcggtagg aaattaaggc ttgatgccac ctactgtctt cggtacaagt
    46381 taattattaa cctcaactag cttagcatgc taaactagtt gagtcaagaa ttatttttca
    46441 gttggatcaa tcaacattct aatatttaat tagaaagaag cttggtcaac atggatacca
    46501 ggtccaaatg ttgttgtgat cgttacattt ttcatgtatt gacctttagc tgctgatggt
    46561 ttagctttta gcaatacatc atggattgct gcaaagtttt caactaattt ttcatcgtca
    46621 aatgaaactt ttccgattgg tacatggata tttccagctt tgtccacacg gtaagttact
    46681 ttaccagctt tcacttcgtt tactgctttt gttacgtcca ttgtaactgt accagtttta
    46741 gggtttggca ttaagccttt tggtcctaat actcgtccta gacgacctac agtagccatc
    46801 atatcaggtg ttgcaacaac tacatcaaag tcaaaccatc cgttttggat tttttgaacc
    46861 atgtcgtcgt cacctacgaa atctgcacca gctgcttcag cttcttttgc tttgtctcct
    46921 ttagcaaaaa ctaaaacagt ttgtgtttta ccagttccgt ttggtaatac aacagcacca
    46981 cggatttgtt ggtctgcttt tttagggtct acgttcaaac ggtatgcaac ttcaactgtt
    47041 gcatcaaatt ttgcaatgtt tgtttctttt gctaaagcta ctgcttcggc aacaggataa
    47101 actttacttg aatcaacttt ttttaatgca tcttgcattt ttttgctctt tttagccatt
    47161 ttggttcctc cttgattgtg gttataacgg ttgtacctcc cacgtgttct atatctttca
    47221 atatagagcc aactgagaag ctgctgtctt agggtgtata gattattcta cagtgatccc
    47281 catgcttcgt gcagttcctt caaccatgcg cattgctgct tcaacgttag ctgcgtttag
    47341 gtcttccatt tttaattcag cgatttctct tacttgatcg ctagaaactt tcgcaacttt
    47401 atttttgttt ggttcgcctg aacctttttc gattttagct gcttttttca atagaacagc
    47461 agctggcggt gtttttgtaa cgaatgtgaa tgaacggtct tcgtatacag aaataacaac
    47521 cggaatgatc aaacctgctt gatcagctgt acgagcgttg aattcttttg tgaatcccat
    47581 gatattgata cccgcttgac ctagtgcagg acctactggt ggagctggag ttgctttacc
    47641 tgcaggaatt tgcaatttaa cgattttttc tactttcttt gccacgagac atacctcctt
    47701 aagtccgtga tgtggttaat tggagatgca tatttctcct cccacgtttt atctgatctg
    47761 aaaaaatcca gacaaataat gtgcaaaaca atgcacactg gtacagtata gcaaatttat
    47821 ttgttttttc aagatttttt tctgctattt ttacattttg cttgaataaa cgctaaattt
    47881 attctaaaaa aacaacaatt tttcgttctc atttcttctt ttatgttaca cttgcgctag
    47941 catggttcaa cagatgaaag caaacaataa cacggttttt tagacccttg tacaaagatt
    48001 cctacataac aaaatgaggt ggagaaaatg aaagatttaa ggtatagaag tgtctttgat
    48061 attattggac ctgtgatgat tggtccaagt agctctcaca ctgccggagc tgctagaatc
    48121 gggaaaatca tacgtagtat atttggcgaa cagcctgatt ctgtagatat ttatctatat
    48181 gaatcatttg caaaaaccta tcgggggcac ggaacagata ttgcattagt tggcggcctg
    48241 ttagggatgg aacccgatga tgaacgttta gctgattctt tgaaattggc atatgaagcc
    48301 ggtatggaag tcttgtttgt tcctaagagt gaaaaagccg aacatccaaa ctctgtaaag
    48361 atgcttctaa aaaaaggaac acgaaagctt tcagtcactg gcatttcgat cggcggtggc
    48421 aatatccaga tttctgaatt gaatggattc aaaatttctt taagcatggg tactcctacc
    48481 ttgatcgttg tccaccaaga tgttccaggt atgattgcta aagtgactag tatactttct
    48541 gagaccaata cgaatatcgg cactatgaca gtgacacggg aatcaaaagg tgaaaatgcc
    48601 attatgataa ttgaagtaga tgatcctcaa gtagaagaaa cggtaaaaag actcaaacag
    48661 ctgccaaata tcgacagcgt aaattatttc gattaaggag ctaaaaaaat gtttttatcg
    48721 attgaagaat tagtagatca agcaaaagct tatccaagtg tagcagaatt gatgattgca
    48781 gttgaaatgg aaatgagtgg ccgttcccgt agtcacatca tcgagatcat ggaaaaaaac
    48841 ctctcagtaa tggaacaatc aatcaacgag ggaaaagaag gtgttacctc cgtcacagga
    48901 attaccggtg gtgatgcaaa aaaattagat gattatttaa atgaggcaga ctttttaagt
    48961 ggagaaacaa tcttaaaagc tgtacgcaat gctgtagctg ttaatgaagt caatgcaaaa
    49021 atggggttga tttgtgctac accgactgcc ggaagcgcgg gtgttgttcc tggtgtgctg
    49081 atggctgtcc gagatcggct tgatttgaca catgaacagc aattggaatt cttatttacc
    49141 gctggcgctt tcgggttagt catcgcaaac aacgcttcta tcagtggtgc agaaggggga
    49201 tgtcaagcag aagttggttc tgcaagtgca atggcagcag cagctttggt ttgtgcaaaa
    49261 aatgggactc ctcatcaagc agcacaagca gtagccatta cattgaaaaa tatgatggga
    49321 ctgatttgcg atcccgttgc aggcttagtt gaagtcccgt gtgtcaaacg taatgcttta
    49381 ggctcttctc aagcttttat ctctgctgat atggcattag cgggtatcga aagtgtcatt
    49441 ccgccggatg aagtcgttgc tgcaatgtac caagtcggac gtcaaatgcc gtcgattttc
    49501 aaagaaacag cagaaggtgg cttagctgtt acgccaactg ctaaacgact tgcggaagag
    49561 attttaaata agagggcgtg ataaagctcc ttaatttcga gtattaagag attagttatc
    49621 ttcataacaa aaaacgaaga gaggactaat tgcccttctc ttcgtttttt attaatccat
    49681 caatttcgta ttttttactt catcgatcgt ttttgttcct gcaagttgca tcgtgatcga
    49741 caattctttg ttcaggtgtt caaaaacaga tgttactcct tctgccccac ctagattcaa
    49801 cccataaatc actggtcggc caatcgcaac taagtcagca ccacttgcca gagctttgaa
    49861 gacatgctct cctcgacgga caccagagtc gaaaatcact ggtacacgtt tgttgactac
    49921 ttcggcaatt tttggcaata cttcaaagga cgcaggacct ccatctagtt gacgtccacc
    49981 atgattggat acccagatac catctgcccc tgcagagata gcgattactg catcttctgg
    50041 cgattgtatt ccttttacga taactggaag atgggtgatt tctttgattg ttttgatgtc
    50101 atctggtgtc aagccttgtt ttgcagctgc ataaatttct gcgattcctt ttccttcgcc
    50161 attaccactt gcactttgtt cgctatacgc agctaagtta ggcattggta atggaaattg
    50221 gaattgattg atgacgtctt cttcacggta accaccgagc gttgaatctg ccgttaaaat
    50281 gatagcttta gcccctgctt ctactgcttt gttcaaaatg aactcattga atctgtcgtc
    50341 tttactcata tataattgga aaaactgcgg tgcgcctggc gctgcatcgg ctgcgtcttt
    50401 gattgtcgta tttgcataag tgctgatgga aaagattgat ccagcagcag ctactccttt
    50461 agctgtatca gcttcccctt tttcatgtgc caatccttgg gccgctgatg gcgcttggat
    50521 aatcggtgta tctaattcaa ttccgaaaac agatgtatgc aaatcggctg agtcgattcc
    50581 gcgtaatatc cgtggcatga ttttctttgt attaaaagaa gtcgtatttt ctttcatcgt
    50641 ccactcatct tcagaaccac cgcgaatata gccaaaagct cctgcttcca tgcgctcttt
    50701 tacacgacct tctagttagg caatgtttac aatatcgatt ggatgttctt cattacttgt
    50761 atgataagtc atagtagatc cttctttcat cttcttgatt acatataaat catactaaac
    50821 ttacaaaaag taagcaaaca tttactctca gatactttta tcgctattct ttccaaataa
    50881 atcgacttac aagctgatct acttcttggt tttcatgaag catactatga gaggcatttt
    50941 cacctgtcac tactttttcc tctatttcat ttccgcgttg cttcaacaac gcatagaccg
    51001 ctaatgcact gtttaacggt actgttccat cactcaagtc tgtttcatct agttgaccag
    51061 caatcaagaa aaatcgagtg gttataggaa tattaccgat catttgctgg taatcttgat
    51121 agcggttaga aacgacagca ggcccctttg ctaactcatc tgtcagagac tgcgcactat
    51181 catcgacaaa atcattaaaa ggcgcgccaa ttgctgttaa tgtctgaact tgtggcaatg
    51241 acgtatcctg tccatacgtt cctaaatagc gtagtgcact aactcctccc atggaatgcc
    51301 ccactatgtt gactttatct atgccgtagt tttctttcag atacaaaaga caagttttga
    51361 tccattctgc ttgattccac tcgttatttt tattatcttc aaacaatact tggaccatcg
    51421 gattgtctgc ttttccagac aaatctcctg ttgcttgtat ttgtccatct gttcctactg
    51481 ttaaaaccaa ttcttttact gcttgctgat ttttttccaa acgccgcaac atccctttaa
    51541 aggaattttc tgtcccgctg tagccgtgga aaaaaagtgt tggaatagtg gttttttcta
    51601 tcactttact ggattctgcc gttttttctt tatcagaaac ttcagtagta tttatcttcg
    51661 tcgtctggca accacttaac gcaaacaaac tgataaggac taaagctgct gcagttgcac
    51721 gtttgcataa atttttgatt ggttctgtca tacacctctt cctttccata tagaaaaaag
    51781 aaagacttga aaggccaatc atatgatcga catttcaagt ctctttatta atcgatttta
    51841 tctacttgat cgaaatcaag ttctgtactg gtttcacgtc caaacatatc gatgttgact
    51901 ttcaattttt ctttttctcc gtcaacttct gttacttgtc cttctaatcc agcaaatgcg
    51961 ccttcgatga ttttcacgac ttcgccaact tctacttcta gatcattttg tcgcaggctc
    52021 ataccgatag aacgcaagat atgattgact tcttctggaa gtaatggtgc tggtttgctt
    52081 ccggctccgt gtgaaccaac gaatcctgtc acaccaggtg tgttgcggac aacataccat
    52141 gaagcgtctg tcatgatcat ttcaacaagt acgtatcctg ggaatgtttt atgaacgatt
    52201 tctttttctt tcccattttt gacttcagtt tctttttctt caggaacaac gacacggaaa
    52261 ataaagtcgg ccattttcat actttgcgca cgagattcga tatttgcttt tactttgttt
    52321 tcatatcctg aatatgtatg caagacatac cagttttttt caaaagattc cattgaaggc
    52381 tcctcatttc attgtaaaat aaaaaaacct cagtacaccc gaagtttttc tttcgcttca
    52441 ttatagcact aaaagtacgt tgtggctagc gagttatttt aaaatccagc caaataagct
    52501 ttggatggct gtatccatta cgtaaaacat caatccgaaa ataatagaag tttcgatgac
    52561 tactaacgta tctttacgga gttgttttct gcttggccaa gttacttgtt tgatttcatc
    52621 ttttacgctt tttagaaatt tcatgagaca acctcccttg ctactgttta tttcgtttct
    52681 ttatgtaatg tatattgatt acaatattta caaaacttat tgatttccaa acgttcgccg
    52741 ctttttcctt cactgactga ttttgtgtag ttgcgagacc cacaaacgga acaagcgagt
    52801 gctgcttttt tctttgccat ttacttctgc ccccttgctg agaatcagac ttatacttca
    52861 taacagtatc acgtaacttt ttacttgtca atttgtgaaa taaactatct attgttttgg
    52921 ataaaattat gatatgatta atttgaaatt aagctaaaat gagacaattg gaggtatagc
    52981 gatgctatta aaaacacttg tatataagaa aaaagatctg acaaccgtta cagagactag
    53041 tacattagaa gaagcattaa aaattttaga aacttccgga taccgttgtg tgccgatctt
    53101 agattcttca ggtaatattt tccgtggaaa catttacaaa atgcatatct atcgtcataa
    53161 agcaaatggc ggtgatatga cgcttcctgt cactcatctt ttgaaaaacg ctacaaagtt
    53221 catttacctg gatacttctt tcttcaaagt attctttacg atcaaagagc ttccttatat
    53281 tgctgtcctt gacagtgaaa atcattttta tggtattttg acacatagta cgttgcttaa
    53341 tatgctctct caatcttgga gcgtagaaca aggaagctat gtactgacaa ttgcttccac
    53401 aggaaagcaa ggagatttgg ctgctattgc aaaaatcatt tcaaaacaca gcagcattgc
    53461 cagctgtatt actcttgata tcgaaaaaga cgaatacatt cgacgtacat tgatcacgct
    53521 accggctaac actgaagcga aagtatgtga aacgatcgtc aaccacttag agaaaaagaa
    53581 ttataaagtg gtagaaattg aaaatttgca aaaccaagaa gcataatcaa aaagcgaaaa
    53641 aagaaaacag ctaatctgct ttcttttttc gctttttata attagacacg cttttcaaaa
    53701 tcaagcgcgc gatctaaata atacatcaat ggtgctgaaa cagccatgac gataaactca
    53761 cttaatgcaa gcgtgccata ggttgtccaa aatgcttggc cacctgttgg aactaacata
    53821 taagcaatca gtcccatgct gatggtaaag aagatcgtgt tcaatactaa acgcatcttt
    53881 acatttttca ctttgttttg caataaagct gtcaggccta aagaaagcaa tgtttggccg
    53941 ccaccaaaaa ccacatccat gataccaaaa ccaaagaagg cattatagac gatgaccccg
    54001 cctaaaactc cccaaagcaa tttacggtta aagacaacta aatgattcaa actttctgaa
    54061 atacgaaact ggatcggccc tgaggataca ggcgcaacta aaatagttaa cgctagataa
    54121 agagccatga tgatgccatt tacaacgatt acggataacc gttgtctgct tttcgttttt
    54181 tgcattttct ctcctctttt ctcctagttt ttttaacgcg ggatggttga tgaaccgcgg
    54241 ttatgtgaaa caaacaataa ttataacaga cttttcattt atttcaatcc gtcttatctg
    54301 cttttaaaaa agcaaaagct taaacaatgt aaacgtttgc taaaaatcgt gggatatggt
    54361 ataaagtaag tgtaaggggt cataagaatt cccagtaaag cagacgaagt aggatctgca
    54421 agaacagaat tgttgtgaaa cttaaataaa taaaaaagga tgtgggaatt tgagcaacct
    54481 cttactagag aacggtctag caagcagctg aagatctaag tggacatgcc gaaccttgga
    54541 acttctcctt acgtaagtaa tggatcatgc gcttgctagg cttttttgtg tttttcggtc
    54601 ttcggacctt tttcattttc ctagacttct gctataataa ccaagaatta attttgtaac
    54661 agattttttt gaaaaaacaa gtttctttca ttcaaaaaat agaaaaagct tgctaattat
    54721 tggttaacca gcaaacagga gtattaaaat atcttgtttt attgaatagc aggaggaaat
    54781 catgaaattc cattatcata tattgatcaa tccatcggca ggcagcggaa atggctataa
    54841 agtagcagaa agaattttac cggtattaaa aaataagcat attgactata cacttcatta
    54901 tagtgaatac aaaggacacg atgctgagat tgctgaaact ttggcaaaag aaacattgat
    54961 tccttggata gaagaagaac agaaaacaat cgatactttc ccccttttga tcatcgtagg
    55021 aggtgacggc accttacatc aggtacttga tactttctat cagatggaag tagaattccc
    55081 agttgcttat attccagcag gttctggaga tgattttgct cgaggggcag gccttccaaa
    55141 aaatcccaaa aaaggacttc agctgatttt agcagctcaa tcaccagaaa aagtccacat
    55201 aatggcttat gaagaaaaaa taagtgaaaa aaaaggaata gctgttaata atttcggtat
    55261 aggattagat gcagctatcg tccatgcgac aaatcattct tccacaaaaa agcgattgaa
    55321 caaatacaac ttaggatcat tttcgtattt attctctatt ctccgtgctt tattcactca
    55381 aaaaggattt ccaattctgg tagatgcagg aggaaaacaa ttaagttttt caaacgcatt
    55441 tttatgtaca gcaacgaagc atcctttttt tggcggagga atcgcgattg ttccaactgc
    55501 tgatgcgtct aaaccagtca ttgattttgt cgtggtcgaa cgaatcaatt tctttaaaat
    55561 cttgtggctc attcttttac ttatacagaa aaaacaattg aaatcgaaat atttccatca
    55621 ctacacaaca aacagattac gtatcgtttc aacaacaccg caacatggtc aagaagatgg
    55681 agaagaaatg gaaaaacgac cttttgatat cacaataacc acaactaaac agttgttttg
    55741 gtgttaataa aagaggtttt gtcagaagct acatctggaa caccgtttat tcggaaaaaa
    55801 ggactgtgac acgacttttg tcacagtcct ttttttacta tgcagttaat tctttgccca
    55861 attgggttgc catcgatagt ttctttcttt acccaaaata aattggatca ttgaaaacac
    55921 cgcagcaaaa gcaactaaga cacaaccatt gaaccaaaaa gattctgcaa atgtttgatt
    55981 gtaactgagt gtttcactga tcaagttatt attttgaaca aaccaatatg ttggtgtgaa
    56041 tgccgctatt ttgctaacca tttctggcaa gtattctgct ggtacaaaca ccccaccgat
    56101 aaagcaactg cctgtgataa aaatatcttt gatcccaccg ctggtttctg agttttttac
    56161 caatgaagtg atacaagccg aaaagctaat gatcggcaat aaaaacaaga aagtatttaa
    56221 aatatgatac cataccgtta tatcccacga ggtgtggatc actaacagaa ccattagaag
    56281 taagaagcca ctgaacaaca gcagcgagta actaatcaaa ctaaaggtta ctttgcgatt
    56341 caagtttcgt ttggtaatgg gcgcactctg gtttcgttta ctgatctctt tgcgattaaa
    56401 tgctagattg atgacgctga aaccgctaaa aatggataga aacaacccat aagacaagaa
    56461 attaaaggtt gtgcccgtca acagcttttt catccgctga ctatatcccg cacttagctc
    56521 aacttctcca gtaaccgaga gtgccatttg tgtctgttcc atagctgcat cgattgtttt
    56581 atcttcttct tgaaacagaa tcagtgtttg caaaaaggtg ttcacttgct gtgtaacaac
    56641 tgttttagaa aaggcatctg ggcgactctg tatcttgatc gtcggcatct ttcctacttc
    56701 gacttgttcg gtaaagtctg caggcagatg taagataatg tttacacgat taaaataaag
    56761 agcatcatcg atttcttttt gtgaggttcc atccaatgta acgactgttt ccttgtcatt
    56821 caaatattta cgaaggccat cgactacagg cgactcttct tcactaatca aggcgatttt
    56881 cgcatcttgc agagaggtat tttcaacatc aatattttgc gcaaaaaaga atgtggcaac
    56941 aacagtgata attacaccta tcagtaaatt cgatttattt gctttaagca atgtccaaat
    57001 cgttttataa agcgtcataa cgcacccctc tttccatcca agcatttcct aacgcaaaaa
    57061 ggaaaataaa tccgatcaac catagaagat tggtataaaa actagccaaa tcagtgtaga
    57121 aataaagttt
//
LOCUS       DAFCNJ010000015        52178 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000015 DAFCNJ010000000
VERSION     DAFCNJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.015"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            330..1175
                     /locus_tag="LNE35_001100"
     CDS             330..1175
                     /locus_tag="LNE35_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6910272.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="LNE35_001101"
     CDS             1456..2676
                     /locus_tag="LNE35_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBK6910273.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="LNE35_001102"
     CDS             2673..5393
                     /locus_tag="LNE35_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6910274.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="LNE35_001103"
     CDS             5377..6321
                     /locus_tag="LNE35_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6910275.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="LNE35_001104"
     CDS             complement(6390..7400)
                     /locus_tag="LNE35_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6910276.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="LNE35_001105"
     CDS             complement(7580..7924)
                     /locus_tag="LNE35_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK6910277.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="LNE35_001106"
     CDS             complement(7917..8351)
                     /locus_tag="LNE35_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBK6910278.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="LNE35_001107"
     CDS             8489..9226
                     /locus_tag="LNE35_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910279.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGDIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="LNE35_001108"
     CDS             9219..10430
                     /locus_tag="LNE35_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910280.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="LNE35_001109"
     CDS             10634..11416
                     /locus_tag="LNE35_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBK6910281.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="LNE35_001110"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="LNE35_001110"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBK6910282.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="LNE35_001111"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="LNE35_001111"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBK6910283.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="tadA"
                     /locus_tag="LNE35_001112"
     CDS             14132..15061
                     /gene="tadA"
                     /locus_tag="LNE35_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBK6910284.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="LNE35_001113"
     CDS             15018..16067
                     /locus_tag="LNE35_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBK6910285.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="LNE35_001114"
     CDS             16064..16342
                     /locus_tag="LNE35_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBK6910286.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="LNE35_001115"
     CDS             16317..16787
                     /locus_tag="LNE35_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBK6910287.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="LNE35_001116"
     CDS             16804..17106
                     /locus_tag="LNE35_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910288.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="LNE35_001117"
     CDS             17123..17494
                     /locus_tag="LNE35_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBK6910289.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="LNE35_001118"
     CDS             17466..17825
                     /locus_tag="LNE35_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910290.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="LNE35_001119"
     CDS             17997..19004
                     /locus_tag="LNE35_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6910291.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="LNE35_001120"
     CDS             19029..20213
                     /locus_tag="LNE35_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBK6910292.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="LNE35_001121"
     CDS             complement(20650..22233)
                     /locus_tag="LNE35_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6910293.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="LNE35_001122"
     CDS             complement(22811..22954)
                     /locus_tag="LNE35_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBK6910294.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="LNE35_001123"
     CDS             complement(23176..23655)
                     /locus_tag="LNE35_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6910295.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="LNE35_001124"
     CDS             complement(23728..24012)
                     /locus_tag="LNE35_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910296.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="LNE35_001125"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LNE35_001125"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="LNE35_001126"
     CDS             complement(24356..25642)
                     /locus_tag="LNE35_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBK6910297.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="LNE35_001127"
     CDS             25780..26451
                     /locus_tag="LNE35_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBK6910298.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="LNE35_001128"
     CDS             26417..26905
                     /locus_tag="LNE35_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBK6910299.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="LNE35_001129"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="LNE35_001129"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBK6910300.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="LNE35_001130"
     CDS             27866..28630
                     /locus_tag="LNE35_001130"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBK6910301.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="LNE35_001131"
     CDS             28863..31076
                     /locus_tag="LNE35_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBK6910302.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="LNE35_001132"
     CDS             31087..31533
                     /locus_tag="LNE35_001132"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBK6910303.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="LNE35_001133"
     CDS             31704..32963
                     /locus_tag="LNE35_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBK6910304.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="LNE35_001134"
     CDS             complement(33052..33999)
                     /locus_tag="LNE35_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6910305.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="LNE35_001135"
     CDS             34393..35685
                     /locus_tag="LNE35_001135"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBK6910306.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="LNE35_001136"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="LNE35_001136"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBK6910307.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="LNE35_001137"
     CDS             37578..38453
                     /locus_tag="LNE35_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6910308.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="LNE35_001138"
     CDS             38719..40602
                     /locus_tag="LNE35_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK6910309.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="LNE35_001139"
     CDS             complement(40677..40961)
                     /locus_tag="LNE35_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK6910310.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="LNE35_001140"
     CDS             complement(40951..41685)
                     /locus_tag="LNE35_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBK6910311.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="LNE35_001141"
     CDS             41814..42437
                     /locus_tag="LNE35_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBK6910312.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="LNE35_001142"
     CDS             42629..43750
                     /locus_tag="LNE35_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBK6910313.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="LNE35_001143"
     CDS             43757..46885
                     /locus_tag="LNE35_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBK6910314.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="LNE35_001144"
     CDS             46904..50098
                     /locus_tag="LNE35_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBK6910315.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="LNE35_001145"
     CDS             complement(50244..50951)
                     /locus_tag="LNE35_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK6910316.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51716..52114
                     /gene="spxA"
                     /locus_tag="LNE35_001146"
     CDS             51716..52114
                     /gene="spxA"
                     /locus_tag="LNE35_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK6910317.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17125 a         9069 c        10798 g        15186 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg gggatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagtaa
    51301 tcattgcttt aaaatactac gcttatataa cagagaaaat aagcgttata ataaagaaca
    51361 gtattattta aaatgacaga aaacattact attaaagaaa taaaaaacat aaaaaatctt
    51421 aagaaatcgg tttcatttag cttttgagat aaaataatag tacagtttta tacgctgttt
    51481 catatgttta gaaaatttga attagatttg aaaactctaa tttttaaatg ataaaaaaag
    51541 cgagaatgct ggttttactg tatattaacc ttagataact ttcatttttt taaaaaaatt
    51601 atgaaaaaaa ggtagctatt taaaagttga ttatgtataa tagaaacaga tgggagatta
    51661 ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt gcacgttgtt
    51721 aacattatat acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt ggttgcaaga
    51781 acatcagatt ccatttgttg aacgtaatat tttttcagaa ccattgaaca gttctgaatt
    51841 aaaagcgatt ttacaaatga cagaagacgg aacagaagaa atcatctcga cacgttccaa
    51901 agtattccaa aaactgaaca tggatttaga tgatctgccg ttacaagaat tattggaact
    51961 tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg attgatgata aacgtctgca
    52021 agttggtttt aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac gacaattaga
    52081 attacgtcaa gcacaattga tggctggatt atagaaggat aatacgaaac ttggaaaaga
    52141 ctggctcttt gtcaaatgat gttggtaaag agaaatct
//
LOCUS       DAFCNJ010000016        48699 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000016 DAFCNJ010000000
VERSION     DAFCNJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.016"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            23..646
                     /locus_tag="LNE35_001147"
     CDS             23..646
                     /locus_tag="LNE35_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910318.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="LNE35_001148"
     CDS             1096..2145
                     /locus_tag="LNE35_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6910319.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="LNE35_001149"
     CDS             2142..3023
                     /locus_tag="LNE35_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910320.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="LNE35_001150"
     CDS             3026..3796
                     /locus_tag="LNE35_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6910321.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="LNE35_001151"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="LNE35_001151"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBK6910322.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="LNE35_001152"
     CDS             5107..6270
                     /locus_tag="LNE35_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6910323.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="LNE35_001153"
     CDS             6387..6797
                     /locus_tag="LNE35_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6910324.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="LNE35_001154"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="LNE35_001154"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK6910325.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="LNE35_001155"
     CDS             9370..9654
                     /locus_tag="LNE35_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910326.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="LNE35_001156"
     CDS             9814..10050
                     /locus_tag="LNE35_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6910327.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="LNE35_001157"
     CDS             10047..10445
                     /locus_tag="LNE35_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBK6910328.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="LNE35_001158"
     CDS             complement(11112..12056)
                     /locus_tag="LNE35_001158"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK6910329.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="LNE35_001159"
     CDS             complement(12352..13296)
                     /locus_tag="LNE35_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6910330.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="LNE35_001160"
     CDS             13517..14731
                     /locus_tag="LNE35_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6910331.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="LNE35_001161"
     CDS             14753..16768
                     /locus_tag="LNE35_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBK6910332.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="LNE35_001162"
     CDS             complement(16851..18224)
                     /locus_tag="LNE35_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK6910333.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="LNE35_001163"
     CDS             complement(18250..18534)
                     /locus_tag="LNE35_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6910334.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="LNE35_001164"
     CDS             complement(18536..20551)
                     /locus_tag="LNE35_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6910335.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21032)
                     /locus_tag="LNE35_001165"
     CDS             complement(20730..21032)
                     /locus_tag="LNE35_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBK6910336.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21121..22152)
                     /locus_tag="LNE35_001166"
     CDS             complement(21121..22152)
                     /locus_tag="LNE35_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6910337.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22359..23234
                     /locus_tag="LNE35_001167"
     CDS             22359..23234
                     /locus_tag="LNE35_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6910338.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23386..24228)
                     /locus_tag="LNE35_001168"
     CDS             complement(23386..24228)
                     /locus_tag="LNE35_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6910339.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24320..26197)
                     /locus_tag="LNE35_001169"
     CDS             complement(24320..26197)
                     /locus_tag="LNE35_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6910340.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26194..27615)
                     /locus_tag="LNE35_001170"
     CDS             complement(26194..27615)
                     /locus_tag="LNE35_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6910341.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27618..31007)
                     /locus_tag="LNE35_001171"
     CDS             complement(27618..31007)
                     /locus_tag="LNE35_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK6910342.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31264..32655
                     /locus_tag="LNE35_001172"
     CDS             31264..32655
                     /locus_tag="LNE35_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6910343.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32728..33375)
                     /locus_tag="LNE35_001173"
     CDS             complement(32728..33375)
                     /locus_tag="LNE35_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK6910344.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33383..34069)
                     /locus_tag="LNE35_001174"
     CDS             complement(33383..34069)
                     /locus_tag="LNE35_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910345.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34075..35148)
                     /locus_tag="LNE35_001175"
     CDS             complement(34075..35148)
                     /locus_tag="LNE35_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910346.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35370..36044
                     /locus_tag="LNE35_001176"
     CDS             35370..36044
                     /locus_tag="LNE35_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6910347.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36049..37389
                     /locus_tag="LNE35_001177"
     CDS             36049..37389
                     /locus_tag="LNE35_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6910348.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37538..38524)
                     /locus_tag="LNE35_001178"
     CDS             complement(37538..38524)
                     /locus_tag="LNE35_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBK6910349.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39248..40279
                     /locus_tag="LNE35_001179"
     CDS             39248..40279
                     /locus_tag="LNE35_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6910350.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40388..41920
                     /locus_tag="LNE35_001180"
     CDS             40388..41920
                     /locus_tag="LNE35_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBK6910351.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            42059..42871
                     /locus_tag="LNE35_001181"
     CDS             42059..42871
                     /locus_tag="LNE35_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBK6910352.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            42861..43346
                     /locus_tag="LNE35_001182"
     CDS             42861..43346
                     /locus_tag="LNE35_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6910353.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            43369..43914
                     /locus_tag="LNE35_001183"
     CDS             43369..43914
                     /locus_tag="LNE35_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBK6910354.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(43987..45630)
                     /locus_tag="LNE35_001184"
     CDS             complement(43987..45630)
                     /locus_tag="LNE35_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK6910355.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(45759..47987)
                     /locus_tag="LNE35_001185"
     CDS             complement(45759..47987)
                     /locus_tag="LNE35_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6910356.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(48020..48499)
                     /locus_tag="LNE35_001186"
     CDS             complement(48020..48499)
                     /locus_tag="LNE35_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910357.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        14821 a         8971 c         9170 g        15737 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatgtaca ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tctgttttag gggccattga acctcttaat
    21001 cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc ctcctagtag ttctatactt
    21061 tttcatcgta caataagaca gtttatgcgt caaaaaacaa acccgattct tacaaatatg
    21121 tcatattttt ccttctgatt cttgaatgct tttgattcct tcttccaaaa ttttcatttg
    21181 taagattgtt tcttcatctt tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat
    21241 cccttcgtag acacggctgt aatcaccaac ctcggaaatt actttttctt catgataact
    21301 cccttgttca tctatatagg tcaatacacc ataatgttcg ggcaaatcga taccaaagtc
    21361 ttcatgatca ggcatgtaaa acattttcag atgttcttct tgtcgatctt ttgtttgttt
    21421 cacgaacatc cctttttttc cgtaaacgac gaaacttgga cgttctttga tgcggaaata
    21481 actggatttt atcgaaattt tcatatttcc atagaaaaaa tccagatcga agtaatcatt
    21541 cattcgtcct ttccctaaaa gttgacgtac atcatagctg atatcatctg gttcgccgaa
    21601 ataagaaaga acttgatcta ctgtatgaca agcatgtcca tataaatagc tgttcgcagt
    21661 agaaaattca tgcgtatttt ctggtacttc cggtctgaaa taatcataat gcatttctac
    21721 ttctaaaata tcccctaatt ttccgctttc gattactttt tggactgtca agaagtcaga
    21781 atcgaaccgt ctgttttggt aacattggat aaatagacct ttttcttttg ctaatgcaaa
    21841 taactcttct gcttcctttg acgtttctgc aaaaggtttc tctactagta cattcttacc
    21901 atgttcgagt gccatttttc catattcata atgaaattga ctaggtgtag tgatgacaac
    21961 taattggatc tcaggatcat cccaaagatc agacagctga tcagtatatt cgatgtcttc
    22021 gaatgttttc cacactggtt cagatgggga aaaaattttt ttgatattga ttttgttcgt
    22081 aagttttctt gaaaaaggca aatgatagcg atttgtactc tttccattgc caagataagc
    22141 gattgtaagc atgagtggtg cctcctttta ttgatgtttt cagtatatcc tagaaaagcg
    22201 cagaaagtat ttataatagc tattctggta aaaaagttca gaaaaacact tgttatcatt
    22261 cggtttggtg aaattaatgt tcaaagtaag gataaaaatc aaaaagatag tagcggaacc
    22321 aagtgtttat cagtaagata gaatttggag gtcagatcat gcttattgaa gaaaaactaa
    22381 caaaacaaga gttgtttacc acgacagaga aacggattgt cgattacatc cgtaggaata
    22441 tcgaagctgc agtctacatg acgattgaag aattagcaaa agctacgtat acttcccatt
    22501 cagctatcat tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg
    22561 agctggggcg ggaagttcat caattgattg ctcagttcaa tcaaacgatt gatcctaatt
    22621 ttcctttttc aagtgaagat agcactcagc aaattgcaaa gaaaatggcg gatttatcga
    22681 tccagtcaat aaaaaaagca caattgcaga ttgaacatga agatttagat caaatcggta
    22741 aagtattgaa caaggcacag cgaatctttc tttttgcaaa aggtgattct caaatcacgg
    22801 caaggaaatt ccagaataaa atggtcaagt tgaataagtt tttgatcatg gctgaagaat
    22861 acagtgattc ttcttggaat gcagccaatt tgactagcga ggattgcgcc atttttatta
    22921 gctacagcgg tcgcatccat cattatgaac gaataatgac ctatttgaag catgtagagg
    22981 cccctacctt gctgattacc ggaaatcagc attcagaaat ggccaaacag gctgatatgt
    23041 gtcttgttat atcgcaggag gaatatgatt ttgctaaagt agcgactttc tcctctcaaa
    23101 ttgccttcga ttacgtatta aatacgttat tctcagtgat atattctcaa aattttgaag
    23161 agaatatccg caacttgaaa aacaaacaag aattgatgca aaaaggcctt cttaaagaat
    23221 atcggaaaac ttaagaaaag atcctcattt agtaataaga aagatagtgt ttgataggct
    23281 ctctttgata tcatcataaa aacaggctga agttttcttt ttcaaacctc agcctgtttg
    23341 tttacggaac gattcgaata gatatattat agaaatgagg aatagctaac ttctgtttct
    23401 tattttgaac gattgacgaa aaagatatat aagaaggagg aaaatcagta tacatgcagc
    23461 taacaatcca tacaggaaaa atcgtttgtt ctcggatact ttctgtactt ctttttgaac
    23521 ttcttttgca tgataaggaa cccggtgccc tctgacaagt aagcgatgac tgttgaccat
    23581 ataaggtgta caagttagga gtgtcacgaa atcctgccct ttttcgattc tcagatcttt
    23641 tgtatcagtc ggttctaccg tcttgatctg atcgacttga taagcgagtg tccgtccatt
    23701 gatttcgata tagaaagaat cacctaaagt cagtttcggt aagtctgtga acaactttgc
    23761 ttgaggcagt cctcgatggc tggaaataac ggcatgtgta ttttctcccc ccgtaggata
    23821 agacgttcct tccaataatg aggcaccttt ttctagcagc acagtattcg tttcatcaaa
    23881 aattggcaaa tggacattga tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa
    23941 ataggttcta tcctttttcg caggttcttt tttcttagta aagggatctg cacctggatt
    24001 tccaccttct tttgctaatt gctggttttt ctttgtcatg ttttcttgga ttttagccat
    24061 gacttcttca ttttctttga ccgcttgttg ctgataatga gagatgatct gctgatccag
    24121 ataattattc agcgcgtcgc taacaaatgg ataagctagt atccctattc ctgagagaag
    24181 caagaaaagc atcagcagat ctagaagttt ccttttcttt ggtttcattt gtgttctcct
    24241 tgtgatgact gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt
    24301 tccaattctt gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag tacagtccag
    24361 caatcagtag aaggactact cctgcaccga tatacacata gattccctta ccgcctgttg
    24421 aaggaagtgt acctttgtgt ttatttggta ttttttcagg agaaatcaac tgtcctgctg
    24481 ttacataaga ttgttcatcg atagtaaatg ctacacggtt tgttaatgga acatattttt
    24541 ctggcgcttt cgtttcttcc agatagtacg tgccatattt tagacctgtc acatcgatta
    24601 aaccgttact tgtggttgta aaaaccgttg ctgattcttt cgccgatacc cagctgacgg
    24661 cttttgtgga tgggtcgatc gataaatatt tcgctgtgtc actatcttga tcacgaacga
    24721 cgaattctgc tccagcaagt gtttggtctg atgtaacgtc accatctact ttaacaaatc
    24781 gtttgccccc agtaacgaca tcgactgacg gtggtgtttg atcatttgta tgcccgttat
    24841 cgacatttgc ttggttagaa aatcctttgg ttggatctgc tttttcgttc aaatgcatat
    24901 agtaaacgaa tttcaatgta ccgccaggag ttaatgaagg aatataattc ggatcaactg
    24961 aaaccgtgaa tccgtctgtt tgctcagtga cagaataatt tactgggtcg atttctttat
    25021 ttccatcata taaggcataa gcatacgttc ctgaagaatc attatcaaat gttaaagcag
    25081 cgtcatgagt atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag
    25141 cgatacctaa tgggatattg acagaaatct catattgaat tttttcaccg attgcgacat
    25201 cttttccatt caattgaggt gttgttttat caactttaga cgtatcattt ttgatggtct
    25261 tttctactgg tgtttggcta tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa
    25321 tcatttccgc attatcagga gcttttactt cttctaaatt atattttcca acttctaaat
    25381 gattaacgat caactggcct ttttcaatag atgcctcggc aaagtcattg ttgccgatgt
    25441 cataagaatg accagtaatg aaatgtttgg ctttggtttg atcagttgtc caagtgtaca
    25501 atccatctgt gacactttgg atgtattttt tgacgcttgg tgttccttct tctttagaaa
    25561 taataaattc tgctccattt agtgcttcgt tttcggcagt accgattttt gtaactttca
    25621 acgttccatc attaccgact gtatttttag ggtagagatg gatagtatct agttcttctg
    25681 tcccgtattt ataagagcca tctgcttgtt tgatcatctc atatacaggg aaagctaaaa
    25741 ccatgtttgc ggcagctgac actccgtctt ttggttcttc tttgatcgtg tagactgcat
    25801 ctttcccatt ttgtttttta ggtaaagata aagtgacatt tccatcagca tctgtcgttc
    25861 ctgaagcaac tggtgtacca ggagtcaaag actggactgc ttgttttgct gcatccacgg
    25921 acgctccttt atctcgttgc gcataaaatt cttgagtgac gttataaact gaaaatgaaa
    25981 tatcggctaa tccttggtat tgatcgaatt cgctcatttc tttcccgctg ttttggatta
    26041 aaggatcggg taaatcagtc attttctttt tatgaaggat gacttgagct gctgtctcct
    26101 ctgcaaacac tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga
    26161 ctcctaacat aacgtttatt tttttatgat ttttcatttt ttcactcctg ttcttttttt
    26221 gataaacaac cagattgcaa agcttgcgaa taccatacca acaacgccta aaagtgtatt
    26281 tttcgtttcg tttgtttttg gaagaaatgg acggctattt tcttttttat tgactacttt
    26341 caatatactg tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa acggaatttc
    26401 tgtttcactt aaaatataac cagaaggtgc ttcgatctct tttaatcgat aacttcctgt
    26461 tttcaacccg gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa gcgcatctga
    26521 ttcgttcttt gtccacttgt atccgttggc tgtttgctgg aggtaatttc ctgctttgtt
    26581 tttaataagg aatactgcgc cagctaatcg tttatctggt ttatttgtct cgattttgac
    26641 aaattgctta ctccctgtat atacagagac tttctttgta ctgacaccaa aaccaaaatc
    26701 cagtgttccg ttgttgttga tttccttgtt cgcctgtgct gtactgctta gctgcatctg
    26761 ataagaaatg gtcagttttt ttccagcata tttttccaac gaagggatcg aaaataaaac
    26821 atcaaatccg tgaggtttct tgtgtatgac aaagccttct gtcagctttt ctccattcga
    26881 tgaaatcgtt aaactattag gtaaaaacgt taatgcttca tccgcactat ctgacaattc
    26941 gaacttttga tagtcaagga tatttacagg aattgtcgta tgcaaggaat aagtgatttt
    27001 ttcaccaatc gtaaaatcat ttctaggctc ttctaatacc ttctcaaaag gtggatcagg
    27061 gtagtcgttt tcttcatttt taggatacaa atggatagtt gaaagttttt gattgttttg
    27121 tccatgtaca ggaagaacaa ctaccatatt ctctgccttt tctttgacga cttctggtac
    27181 tttggattca atgaaaagat acgctttatc tcttttttct tgatctttag cggccagaga
    27241 aaaagaagcg ataccatctt cgttgttaag agttgtagtt gttgcttctg cggtaaacat
    27301 accggaagac gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt
    27361 ttcccgtaga tggtaaaaag aatctgtgac atcataaact tggaatgtga caccatttaa
    27421 tcctcgatac gtttgtaata aagctttttc ttgtccgtca tttggatgat ttttcggcag
    27481 ttgcccgttt gggaaaagca atttgtgtaa agtaatctga accatttctg tttctgtttc
    27541 tgttgctacc tgagtaaaag ctggtttaaa tagtaacaag aagagacaca tactaagcca
    27601 accaagtttt ttcatattta gctgccccct ctttcttttt tcaaaaaaag ataccaaata
    27661 gaaaacgcac aaccaatcat ccctactaga tagattccta aacggccaat tcctcccgtt
    27721 tcaggtaatg gtacttttgc ctgattcgtg atgtctaaag aaatctggtt gtttttggct
    27781 cctggtgaca gaacagattc atgatcttgt ccatccactt gaattgaccc atcttcgtgt
    27841 ataactatag taactggctc ttttagacct tggtatcctt ctggtgtaaa agtttcggtc
    27901 agcgtataag ttccaggttt taaattttcg aatagaaagg tatctgtttc ttgtccatct
    27961 tttggcgatt ctaagtccat ttctggtccc tgcagtctga attttgctcc ttttaatgtc
    28021 tgtccgttgt cttctttttt gtgtaccgtt agatcaaacg cttttagtcg attttgatga
    28081 gtgagcgtcc atacattatt tgtcatctcg gtattttttc ccgtgtaaat gatttttcca
    28141 tatttatcga tttggaattc ataatttccc ggcgaagtat catatccggc aggaccagct
    28201 gtttcaacga ctcgatattt accaggtgtg agtgaatcaa aactggcaag acctttttca
    28261 ttcgttgttt ggctgtcaat tggctgataa gtaccatttg cttctttttt ctgtagggaa
    28321 aatgctgctc ctttaagatt tatctctttg tccgtccctt gcatcgcata cttacgtact
    28381 gctacaggaa cttcaacaaa aggattttca atagtcaact ggatcgtatc atcggaagta
    28441 gtgactgttt ttccatcaat ggttaccgta ccatcagaag cgatcttgat ttcccaagta
    28501 gtctttttgc ttaatccatg cccttctgga gcttttgttt ctgtcagcgt ataggtcatt
    28561 tctttttgca atttgacatt ttctggaaga gaataggttc cgtcgccatg atctgttagt
    28621 aaagtatcaa tagaatctcc tgttaatttg aaaacagcgc caataagatc cttttcacct
    28681 gtagaggaat ttttcgttat tttcaagttt aacggtgtga attgtttagt atttttccat
    28741 gtcattgcat ctatttgttg agaatcgtat cctggtacag gtaattcttt gattgtttgg
    28801 taactgaatg cttgaccttg attattgtat tgaggtagtg ataatatctc ttgataactt
    28861 tctccgctat ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct
    28921 ttagctggtt taatgattcg aatatatccg tttttccaag ctgcagcatt tgtcgtatgt
    28981 tcccgttgaa tctcaaaagt aacttgatct ggacgatcag ctagattgtt gtcaaattct
    29041 tcccataact ttttgatgtg aagactgact ccgggagctt tagcagatgg tataccgaat
    29101 tcagctaatt cattggtatc aatgtttggc tggaaagttg tcctgccgtt catttgatac
    29161 cagaaatttg gatggaagtc ctcattttct gtttggattc tcacttggta atggatttgg
    29221 atttcttggt cttttcctaa atagatctga ttgctcttga tggtatttcc ttctatggaa
    29281 atagatggag tgaccgttgt aggacttgtc cccacactct tgactgaaag tgtaccaggc
    29341 tgataaatga atggttctgc gattggatca tttatttgtc cattgcttac agttgctgag
    29401 atctgtacag cttttttggc taggtattca gagatatctg ttgcatgatc agctgattca
    29461 tagtaaagat cgcctttttc atctgatgaa accatttgtc gcatacgaga ctctacttct
    29521 gcttttgaga gaccagctgc cggatcgctt tgaagttgga tgccaagacc atgtatttcg
    29581 attcctcgtt ctttgagtgc catcgcttct ccgatggttg cgataaacgt actgtcgatt
    29641 cttctggata gattgttttg gtcaggtgca tcatagattc ttgagattag agaagtattt
    29701 cccggccgat cttgcgtatt agaaaactga gttccgtaat aattgctgct tgattgtgcg
    29761 tgtacccgct gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact
    29821 tttttatgtc catttggaac ggatagcatg cttcctgcat ctcttagtgc tttttgtgta
    29881 aaagtaccac catttgtccg tgaaggtgta atggatttta cttgattttt cactgaatca
    29941 aatgaaccca tctgtactgc accgttactg tagctatatc cttcgcttga gtagccgaca
    30001 tatcccatat tgattttgtc tgtgataccg ctatctgcta aagtatcgac aaaacgatcg
    30061 acaccaatct ttacttcacc gatccgatta ttgtcgttca tacttcctga ccagtctacg
    30121 accaatacga gatcaagagg cgtgatatct ttttgtacat ttccacgagc attcaaataa
    30181 acatcaaaca atccgggtgt acttgtttcc tttgcatact ttcgtatcgc atagtctgct
    30241 tgattaccgg ttcctccata ttcgatatac gaatgggtcc gatcattagg atctccattc
    30301 caagaattta taccatccca ttgatttgtt ccgtttttat tgccttgatg atcaagaaca
    30361 tttgtatttc ctgtaggttg ccaactggct ttaggataag tgccctgatc atctgttgta
    30421 tattctgtag gaatcgtttg attagatggc acactcacag ctggcaagga taatgtaggc
    30481 aatgtaaatg ggctgacaga attttcgttt tcttttgatg attcttcttc tatttcgtct
    30541 ggctttggta ctgacacatc tgtatctttc ttattttctg tctcatcaat aatgtcatat
    30601 tctttattat cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg atcgattgga
    30661 aaactcagat cgatagtttg tttagtgttt gatgagattt ccaacgtaat tttcccgcta
    30721 tttacctgat aagcatagcc atctccttct tttctttcca catcacttaa gtaattttcg
    30781 actagaccaa tttctttttc tatcttttca gcactggtgt tattcgcatt tagcttgatg
    30841 ttcagtgctt tttttccaga ctgatccgaa atgacttcag ctgaaattgt ttctaaaact
    30901 gttggatcat ctgtagtgtc tgctattgca gataatggca caaagtttga taaactcaat
    30961 attatcagca tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt
    31021 tttctttttt tctgttttga cgtgaatagt gaaaattatt aaaataaaca ggcgttacat
    31081 aattttattt attttaatat gataattatt atacgaaggg cgctaaaggg aatcacacca
    31141 attttcatac caattggttt catttatgaa gttgattcct tcgattaatg agcgtttcat
    31201 tgaaagaaaa gggttgaggg agaaaaacat atgatcaggt tgttggaagg aaactataga
    31261 aagatggaaa aaatcattcg aattctgtcc atttctgaca agtggatgac taaaaaagaa
    31321 cttgccaaac gaattggtag ttcagaatct acatttgtaa ggtatatcga agagatcaaa
    31381 atccgctggg gaacggatgt aacgattcaa tcttcacaaa aattggggta tcgtcttgaa
    31441 catttgaatg taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac
    31501 agtcgtttat taaatgagat tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa
    31561 aaaatttcca tcagccggtc ttcttttaca cgtaagttga aacaatgcaa tcaaatttta
    31621 gaaagctatt tattaaaagt gatcgttgac caaggttttc agttaactag ccttaaaagt
    31681 gagctccagt tgcgtatctt tgttaccttt ttctttttaa gttactacgg aagacgcacg
    31741 ttgccatttc caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa taattgtcag
    31801 atgaatttgt ttcaggaaga aagcccatat gagcaatctt tctttattat gtattttgtt
    31861 gtgcatctca tgagagaatc ccaaggaact ccatttgaat ggaaagctgg agactcttat
    31921 gtacagttga taaatatcga taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa
    31981 ttgaagtaca gcagctatat aaaagtagtg aactattttg tcaatacgat tttttcagaa
    32041 ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat gtcctactgt
    32101 tcgttttcat ccgctgattg caaagaaaaa aacgaagaat atgttgttca attattaacg
    32161 caaatttatt ttttctcccg tattttccca tacgatacga ctcatttgac gatccgtgca
    32221 acttactttg cacagcagat gcaacaccgc cagatacgtc tatatgctgg atttcgtgca
    32281 gaacttcaat tttattccac tctactgaat cagaatctga tttattattt atctagctat
    32341 atcttttgga tgcttatcgg taatcctgaa attcaccatt atacttctga taaaaaaata
    32401 ctgattatca gcgacttaag tcttcgacat agtcaatata ttgaagaata tatcagtgat
    32461 attcttgctg tgcataaaat tcactctgag acaactgcta ttactgaaga tcagctgtcg
    32521 aaatacaatc ttctcgagta tgatctgatc gttacgaatc aacccatttt gaatgcccat
    32581 gttcctcata tattgattga tgatagtgtg tcattcgcaa atgaagaaga actcatccgc
    32641 ttattcgagt tatgaataat caaaaaacag ctatgtataa accaccctga taaaaatttc
    32701 aggacaaata cacatagctg tttcttttta ttgtattcct tcagcaatcc aacgagattt
    32761 atagcgaaag tcttttaaca actcttcttt tgtccaatta tttgcaatga gatccgtcaa
    32821 agtgttcatt acgagggtca tggaaaagta taggaaacta actgcttctt ccgacgattg
    32881 aatggagaaa tgattgattc ttaataattc cagccatttt tcgttcattg gagaatcagc
    32941 cgatagttct ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca agtataacct
    33001 ttgccaatag atactttcgt gatctaagtc gcagcaccac tctaaatatt tttcgatccc
    33061 caaaaagaaa tgttgtttgt tttcgctaat gaattttaag atatcttgat ggactatgtg
    33121 agaactgctt ttgatcgtat acaaataagc atcttccaaa tcttgaaaat atttataaaa
    33181 agcaccgcgc gacatcccca tcttgtcaac gatttctgag actttgactt ggctgatgtg
    33241 ctgtgagcaa aatatctcca gtaataggtc atcgattctc tgtttctttg cgttcgataa
    33301 attgaaaaaa gtttcttttg gcatccacat ctctccttta tctctgatta tacgtggata
    33361 gcccttcatt gtcatgaagg attcattcct tctttttctc ttcttctgtc aagtacccat
    33421 cttccattcg ataaacgcga tcactccatt gaatcatacg gacatcatga gtaaccataa
    33481 tagtcgcttt ttgtttctca tgagcttctt taactagtaa tttgacgact tcatatgcat
    33541 gatctgtatc caaactagct gttggttcgt ctgctaagat caagcttggc tcattatata
    33601 aagcgcgagc aattgccact ctctgtcttt ctccaccaga aaggtcttta ggataactat
    33661 ttttaagtga ctggatatct aaagattgca gcaaggaatc gatttttgtt gaatcttctt
    33721 tttttgaagt aacttggtca actagttcaa attgttcctt taccgttaaa aaaggaatca
    33781 aattagagct ttggagaata aatcctattt ctttaaagcg taaatctgct cgtttttttt
    33841 ctggaagctc agttagtgag tgaccgttga tcactatttc tccggaagtc ggtgtttgca
    33901 atcctcctga aatggtaagg aaagtacttt tacctgaacc agaaggacca atgatgctga
    33961 cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa agctgagacc tttgtatgac
    34021 cagaaccaaa tgttttggta acttttttca tcgttaatat ttttttcatt tttattaacc
    34081 tcctattgct gtaattgggt ctactttggt tatcgtacgt atcgaaaaca atccgcctag
    34141 cattgcgaca atgatcaaaa tcccactata tagcaaccat tgtgaccaaa aaacagcaaa
    34201 aggcatcgcg cttggtaaaa tcagactagt caaataagca aacaaaagag caagcagaac
    34261 gcctaccacc ccaacaataa aggactgtgc tactaaagat ttagcgatga agaaattttt
    34321 gatcccttgt gctttcatta ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat
    34381 gaaaataccg accacggcag caaccactaa gaacaaaaag tatatcatcg catttaaggt
    34441 catattttgg gcagaatagc caggaatact ttcaatgaat gtcccaatag ttaataactg
    34501 tagacccttc gatgcttttt ctttcgaaat tttagctgaa tcctttatta cgatcccatt
    34561 gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt
    34621 atctagggac gcatagatga cgggagaaac tgtgtaataa gtttcaggaa agataccgac
    34681 aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta tctccaattt tatagcctga
    34741 atcagccaga ttttgagaga tgatgatttc attctttgtt ttaaattcat gccctttcgt
    34801 gagttttggt aataaaaatg cttctttcgt tgtgccgaaa actgtaacat tttctttgtt
    34861 ttttcctttg attgctccgc tgtacaatcc gattggtgct ttctctcctc cttcaatgta
    34921 tttcagatct cctcttgtta attgtgaagc tgcaaatact tgatttgcat cctccgacaa
    34981 aacaatctct tgtgcgtccc aatcatcgat tgctttttta aactcttctg aaagtccatt
    35041 cgctagtcct gacaacatga atacgacata cgcaatcaag aacataatac cgacgatcaa
    35101 gccatatcgt aatttagaat agcgaatttc tttgatagct agaaacattt gtttttcctc
    35161 cctatgatag attgatagtg acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac
    35221 aatttgtcac ttgtgttgtt tttttcttat caataaattg tctaagacat ctttcgataa
    35281 aaccttaaca tgagtcataa aaaatttatt taagccttat ttatgctttc catgataaac
    35341 taaagtgacc aaatcaatga ggtgggaaaa tgattaaact attagtagta gaagacgata
    35401 aagtattatc caataatata aaagaaatac tcgctgcaat cggcgagata acacaagttt
    35461 atgatggtag tgaaggactg ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg
    35521 atttgatgat gccggaaatg aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa
    35581 tacagactcc agtattgatt ttgactgcaa aagatagttt ggaagataag attgaaggat
    35641 ttgaacgagg tgcagatgat tatctaacaa aacctttcta tcgagaagaa ctgatcatgc
    35701 gcgtgaaagc attattgaaa cgctcgctag gtctctttga tgaaaatgtt ctgaagtata
    35761 aggatatcag ttgtgattta aaaagcaatg aagtcttgta taaagaacaa gttttgccca
    35821 tacaaggaaa agagtttgaa ttgctcgttt attttttaca aaataaggga atcattttga
    35881 caaaagagca gatatttgac cggatctggg gatttgattc tgatacgacg attacagtta
    35941 ttgaagttta tatgagcaac ttacgaaaac atctgcgaag tactggattg gataaagaaa
    36001 tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat gaatggagat
    36061 attacacgaa agcaacgtag tcgctttttt gcgacaaatg tagcagcatt tgcttttatc
    36121 ttttttcttt tgggattcat tactttacag atattaaacc aatctgcata cagagaaaca
    36181 gatctgtcat tagagaaaat aaattttgat tctcgaacag tacaattaga gatacaaaga
    36241 tatcagcaag gcgatccctt tttaaacccg actcaaacgg aacaaaacag aacaaacatg
    36301 gaacagccat taatggaacc atcaaatgac cgttttaaca ctcaagtgat cttgtggtct
    36361 aaaaatggtg agatattgaa taaagcagct attggtgggc gtttcaacca aatcaaagaa
    36421 ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata ttgaattaaa tgaagacagt
    36481 ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc
    36541 gttgcctaca tccaaatttt agaaaataca aatcaaatca agaattcatt agcgactttt
    36601 cgcacgatta tgattttgtg tatgattgtt ttttggattc tctctattgg tatcagttat
    36661 tacttgtcta gtctaagcat gcgccctatt attctttctt ggagaagaca aaaagaattt
    36721 gttgagaatg cttctcatga attgcgcaca ccgttaacga ttattcaaaa tagtttggaa
    36781 catcttttta ccaagcctaa tcatactatt attgaagaat ccgaaagcat cgctcaagca
    36841 ttgtcagaga cgaggcgcct cactggattg acttcggatt tgctgacaat cgctcggaat
    36901 gattctgatc agcagctgct ttcaaagcaa atgatcaata cacaagaata tatcgaacaa
    36961 cttgttaaac catttcaaga gatggccatc atagatggaa aagagtttat tctccataac
    37021 gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt ggttattttg
    37081 ctggataatg cattaaaata tacgaatcca gaagataaga tcattttgga ttcatctgtt
    37141 aaccataaag aatggctgat tcacgtaaag aatactggcc cttctatcag tgaagaagat
    37201 caagcaagga tttttgatcg attttatcgg gaagatcaat ctcgggcgaa agagactggc
    37261 ggatacgggc ttggcttggc aattgcaaaa caggtaatcg aacagcatca tggacagatt
    37321 tctgtatcca attatcttcc acaaggagtc gattttaaag tccaattgcc gttgaagcct
    37381 aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt
    37441 tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcttta
    37501 agctgaccac ttctgtcaca acctctttag cttacgctta cgatttttta ttgcggtctt
    37561 caatgtgtac atcaccgctt gtgtcaatat ccaattcttc tcggctaaca tcttctgtca
    37621 ctttttcaac atcttcatgt tcttctttat gaatcccaac ttcttcacga ataactgtat
    37681 gtttgcttac atctacttgc tcttctttga ttgggatagt aatcgtgtca tcttccaatt
    37741 tttcgtctgt tccctgtgaa gattgatctg ttactggttt gcgttcgatg acgatttctt
    37801 cacgtttgac cggtacttct accgtttcag tatcgttgac cacatgtttg tggatgtcga
    37861 cttctccagt tgtcacatca tgtgtattta catctaattt ttcttctttt agttgaattg
    37921 tatcatcatc tagatttggt ttgcgatcga tgtcttcagt cttgcttgta cccattgtat
    37981 cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt catattcaat ggatccgttg
    38041 tattttgaaa gcctgtcgtg ccacctacgt ccattggatc tgttccagtg ccagtttcta
    38101 atccagtccc cattgtgctt gctgggctaa cagtatcttg tgtatccatt gtatcttctg
    38161 ttgtttctgg ctgacgatag ccttttactg tgatgacaaa ttttccatca gaaatatctc
    38221 ctctatattg atataaagga tcattttctg gtgtagccgt ctcagaatca taggtatcaa
    38281 aagaaaaagt atcttttatt ttttcccata aagaacggtc ttcttcacca tccatccgat
    38341 tttcatctgt ttcaacatct ataccagaca atccgtcaaa tcctagtcta gatgctgctt
    38401 catcagtagt ataaataacg atatcatctc gttcataccc ttcgttacgc aaccgttcaa
    38461 ttactgataa tgcttcttcc ctcgtactat agcttccgat gaccgttaaa tttttctctt
    38521 ccatgtaaat tcctcctagc aaatagattt atgattggta tattgttact tataaattta
    38581 ttctatagga gacgattgtt ttctacaaat atttagattg gaatttgatt aaaacaattt
    38641 aattgaaatt tgaaaacatt tatgttaatg aaagtcatat tctaataaaa tgtcatttgt
    38701 ttctatatga atttttatga aaaatttcat ataatttcca ctcgttgtga tggtttaatt
    38761 tatccaatcc atcaataagc aaacaactta tataaaaaag gattaattct caatataatt
    38821 gctcctacca tagaataaat cttcatgtaa agtaatgaag taccaactat ttctaatact
    38881 tctttaagaa ttatcccaac aatatcgtta gacaaaccat tattgaataa atggagagcc
    38941 aatcgattct gaaaaaatgt tttctaattt ttcggaaata aaagaaagag cctcctgcca
    39001 ttagaacgaa ccaaatccat ttcattgctc atgaagtaac cacatagcta aaaagaggat
    39061 aaatcaatta aaagatcaaa agaatgactg cactaataaa ataactaaaa atgtatcttc
    39121 ttacttcata tcttttagct agtacttagt taaaaatttg cttcctaaga tcaagacaaa
    39181 ctgtctatct tcatttgatg aaatgcggta aaatatagaa aatgaacact attggaggga
    39241 atttgtaatg gacagcaaaa ctagaaaaca actgcaagaa aagttaaagc aacgtacaca
    39301 cttgctccct tccgtctttc aacagttttt agaagaaaat cgttcttccc tttcattagg
    39361 cagtcgctat gaatacgcaa aagattttca tctgtttact gattatttga gaaaagtatt
    39421 ttctgaagaa gtggatgatc agctgattat ccatttagaa aaacaagatt atcagaattt
    39481 cttagctaaa atctatcgac atactcgttc gttccaaact acagcgaacc aagaaacgga
    39541 gcaattgttt gaaaatagct attacgggat ttcaaggaag caatattcat taaatcattt
    39601 tttacgattt ttgtatcaaa aaggactgat tgaagaagag atttctcttt tgggtacaag
    39661 tttaccggaa acaaccaaag cagctccgcg atatatcgat gcagaacttt tcaaagattt
    39721 tgagcatttg tttatcccga ttgaaggctt tcaaacaagc agagaagcta gctttgaaga
    39781 aaagaataga ccgaggaacc ttgcaattac tgccattctt ctgtattgcg gattaaagat
    39841 ccatgaagta gtagaactta aacgtaaaga tgtatctttg accaagcaga ttcttaatct
    39901 gaatagaaaa gaaaacagac tgaataaagt accaatacca aaagaagcga tgccatttct
    39961 ggagaactat tttaagttaa cagcacaaaa tacggacgaa aattcttttg tttttcgttc
    40021 ctcacatgat cagcaaattt ctcctcggac tattcgccaa attttgagca aaattacagt
    40081 atataaaaac gtatcgccag aactatgcag aaaaacatat cgttattatg cagaactata
    40141 tttagatgat gctgacgcgg taaattattt atgtggtaat cgatacctct catcccattc
    40201 ttttcaagat atctgggaaa agatggttga tttttcttat cacgaattag cggcattcgt
    40261 tcgggtttct tccatataaa acaaatgtat gatctttttt tcacaataaa ctatgttatt
    40321 atataaagga ttatgttacg attatcttga atttgtatat tacttaacaa aagataatgg
    40381 agtgtgaatg atggagacca taacgtattc ccctttgaac ttatttacga attttcaaga
    40441 agctgcagct aacttttccg agaccccgat tatctttgat gagccacttt cagctttccc
    40501 agaattagga cttgaaacaa cttacaaaaa atgctgcgaa agtgtgttga aacgggctta
    40561 tcaattggct catcttggag tgaaatccgg agacaaagtt attatttata agagttccgc
    40621 ttttgacaca taccttctag ctgtttctgt gtcttacctt ggtgcagtgc ctgtcatgac
    40681 ttcttatcat ctaccaacaa caacaatgga agtatttatc gatcgtttag aagacccttt
    40741 cattttattt gatgatgaga caaaaaagcg agtgagagaa atttcaaatg gaacaaaatc
    40801 aaaacagatt cctattcttt atttaatgga acaacctgct ttgtctgttt ctcaatcatt
    40861 tttagacaaa aatgaaattt cttatatgac ccatacttct ggtacaactg gtatccctaa
    40921 acttatttgt cattctgctc attctatggg atggcggacg aaatggcaaa aaacagtttt
    40981 cacaaaaatt tcggaaaaga aattaatcgc ttttcacatc tctccagtgc attccagatt
    41041 caatattggt atctcttctt tgatgtccat gggatttcct atgatgcctt tagcaaacgc
    41101 acaaagttca aaagttgttc atatgttgga agctcaccgg ccaattgcac tagagaccca
    41161 tccgaataac tttgttcagt ggcgttttac tgctaaggag catccagaag ctttttcggg
    41221 tatccgatac tatcactcca cttttgatgc tataaacaac cagacaatga agacattttt
    41281 gcgaacgtcg atggcacagg atgcggtatt tcttcaagta tatggccaaa gtgaatgcgg
    41341 accaatgatt cttaaggctc atacactaga gagtttgaaa acaagtgatg caagagatat
    41401 gggcgttgga ctggaagaca tgacaagcgc tcggattaca gattcagttg ggaatgtcct
    41461 acctgaaaac acagatggtc atatacaatt actgtctaaa ggacgggctt tgacttatta
    41521 caaggaagat gcacggttcc aggaaaatgt ttatggcgat tggtgggata gcggagatta
    41581 cggttttatg gataagcatg gccacctatt tttgaaagat cggcaagtgg atttgatcga
    41641 aacgatcaat agtacattag ctattgaaga ctttttatta gattcgttag attttttggc
    41701 agaagtagtg attgtaaggg ataaaaatca ttctccacaa ccgattattg ctttagctcc
    41761 tgataaagaa atggattgga atcgatggtg ggagcaagtc catgaacttc ctcgcttgaa
    41821 cgtccctatc atccgtgatt ttgatgcaat cccgcgaaca gctacaatga aagtgcaacg
    41881 gcttcaaatc gagaaagaat taaaatcaca aaagtcctaa ctatttaagg aaagaaagat
    41941 ccattttggt ctttcttttc tttttgtttt tttaaattgg atgagataaa aatatgccaa
    42001 ttggctgttt ttcttttttg aaaatttcgt tatactctta ttcatggagg aaaatgttat
    42061 gaaaagaatc ttgacaattg gcggttctga tccatttgct ggtggaggaa tccaaacaga
    42121 tttaaaaact tttgagaatt ttggtctatt cggactaagc gccttgactt ctataggggc
    42181 actagatatt aatgagcgat ttatgctgga atctatatct ttggaattgc ttaaacgaca
    42241 gttagtttcg attgataaaa tgacttcatt agatggtatc aaaattgggt tattgaattc
    42301 aagtgaagca atcatggttg ttcgagactt cataaaaaca aaacaaaata tcccaatcat
    42361 tttagaccct gttttggcat tcaaagaaac tgaagctgtg atcaatcaat tgtatatttc
    42421 gcagctcatc gaagaacttt ttccacttgt tacgatgatt acgcctaatt tggcagaagc
    42481 tgctttactt tctggtcaag ctcttccctc ttcgttaaca gaaatgatcc atctcgctaa
    42541 agaactcact gcttcaggaa caaaaaacat tgtgatcaaa ggcggcacaa ggatggctgg
    42601 agatgaagct gtggatcttc tctattcaat tgatcattat gaggtatttt ctcataaaaa
    42661 aatcgacact acaacagtaa acggggccgg ttgtgccttt tcttctgcaa taacagcgaa
    42721 tcttgtgtta ggggcagatt ttaccaaagc agtagcagaa agtaaaaaat ttgttgccca
    42781 ttgtatttct aatggcgtgc tgatgaacga tcataccgga agtgtctggt ctggcggtat
    42841 tcaaaaagga ggaatgagaa atgaaaacta agcaattaac gatctatgct atgctgattg
    42901 cgttgacagt tgccctttca ttaacaattt tgatccctgt accggctacg aatggatttg
    42961 tgacattatg cgaggccggg atctataccg ctgcttttct ttatggtcca acgggtggat
    43021 tagttgttgg tgcttccagt ggtctattga tcgatttgat ttcaggatat ccgcagtggg
    43081 cagttttttc attagtgatc cacgggttac aaggatatgt tgctggttct ttcagcaaat
    43141 cttctactcg tatgtgggga ttagggcttt tattaggaag cttagttatg atcatcggct
    43201 atggtatcgc cggttggatt ttatatgatt ggccggctgg agtggcttct ataccaggaa
    43261 acatcttcca aaatctgatt ggaatgggac ttgcctttcc tataactgca agtcttcaac
    43321 gtattcaagc acggaagcaa tattaattga aaaagaaggt tttagaaaat gaagttgtca
    43381 gaaaaagaat taaaagacca attgactgaa atagttaatg acatattggc tgaggcacac
    43441 ttgaaaaaag gtgatctttt tgttttagga tgcagcacaa gcgaagtcgt tggaggacat
    43501 attggaaaaa attccagtgc ggaagttggt caatggatca tccgtacgtt aaaagagttg
    43561 ttggatccta aagaaattgc gctagctgtc caaggttgtg aacatttgaa ccgagcatta
    43621 gtagtagaac gaacagtggc agaagcaaaa aatttcgaaa ttgtctctgt tgttcctgca
    43681 ctacatgctg gaggtgcttg ttctgttgct gcttttgacc agttcaatga tcctgtcgaa
    43741 gtggaacacg tagttggaca agccggtatc gatatcggtg acacatcaat cggtatgcat
    43801 atcaagcatg tccaagtacc tgtcagacct cgtttgaaaa cccttggaca agctcacgtg
    43861 acagcccttc gctctcgtcc taaatatatc ggtggtccta gagcgaatta ttgaaatgag
    43921 taattgacag agcaatctga aaaacttcct cgcaaagtcc aactaatgaa ctttaacgag
    43981 gaagttttac ttgtttgttt tatataaccg agcttcataa ggtgctagta tgcctttagc
    44041 ttcaaattct ccgctagagg aatttgacaa gagtaatgtc caggactgat ctgcttcttt
    44101 tggaagttca tagtcagcaa gtgattctgt taagtttacc ataataagat attgttcatc
    44161 ttctaaatat ctttcataga cataaagttg tggatgttca tgaagatatt ctttataaga
    44221 accgtatatc agacctttat tggtttgtct aatgcggatc atgttcttat agaattgcag
    44281 gaccgactct ggttcatatt cttgttgttc tacattgatc gttttggtat ttggattagt
    44341 gatcagccaa ggttcatgtg tagaaaaacc agcatattgt tccgaagtcc attgcatggg
    44401 tgtcctgctg ttgtctctcg ttgtttcacg aatgatatcc aatgcttctt ctcttgtctt
    44461 accttccgca agcagccttt tataaagtct tttcgaatct actgcatcaa cttgatcgat
    44521 cgattcaaag ggcatattcg tcattccgat ttcctgtcct tgataaataa atggcgtacc
    44581 ttgcaaaaag aaatacatca tagcgattgc ttttgcagac atagcccaaa aatcgggacg
    44641 agtatctcca aacactgaaa cggcacgagg aacatcatgg ttttccatat ataaagcgtt
    44701 ccatccgata ccatccaatg aagtttgcca agcgcttaat gcatgtttca atttaaggac
    44761 atctaattgc ccttcttttt cttgctgcca gatatggata tgatcaaatt cgaatatcat
    44821 atcgaaataa ccgtctttcc ctacccattg tggtccttct tctgcagtga caccacttgc
    44881 ttctccgaca gtcatgatat cgtactcttc aaaaacatgt ttcaaatcgg tcaaatgttc
    44941 ttcgattcct gagacatttt gaaagggtga gaaagggttc tctgtggctt ttactgaata
    45001 ttcatctttt tttacatgag aaatcgcatc taaacgaaat ccatctatcc ctttatccaa
    45061 ccaccatcgg atcatattga atagttcttc tttcaatttc tcgctttccc aatttaagtc
    45121 tggttgttct tttgcaaaaa catgcagata atactgtcca gtttcatgag aatactccca
    45181 agcagaaccg ccgaaaatcg atgtccaatt attcggttct ttgccatcta ccccgtcgac
    45241 ccaaatgtaa taatcgcgat acgggctttc tttagatttt ttcgattctt caaaccattg
    45301 atgctgatcc gaactatggt tgatgacaag atccataatc actttgatcc ctaattcatg
    45361 tgcttctttt agcaataaat caaaatcttc catcgttccg aatctctctt ctatatcttg
    45421 atagtcactt acgtcataac cattatcgac aaatggagat tgatagatcg gattgatcca
    45481 gataaagccg ataccaagat ctttcaaata attaagtttt tcacggatcc cattgatgtc
    45541 tccttgacca tcgccattgc tatctttgaa actagcagga tagatttgat agcctactgc
    45601 ttctttccac catttttgtt cttgtatcat atcgtttcac tcctttaacg gtccctcttg
    45661 attataaccg attctatttt agaagtatcc ttgttaccgg taactagaaa cgaaaagctc
    45721 aacgaatctg ctaagtgcgt tcgttgagct ttattttatc atagatagcg ggcaatcagt
    45781 tgaatcattt gcttgggaaa ttcattcaag tcagtaatat ctacaaaacg actttcaccg
    45841 tagatcgact tgatttcttc tttgtcttgg ccaatagctg cggagacaaa taggattcct
    45901 tgacgttcat attctttcaa aacagactgg atatcttctt ttgctttttt gcctgtataa
    45961 tccggcaatg cttttggctg accgtcacta atattaagca acagtttggt tgttgcggat
    46021 tgtctgttta atttttctgc tgctaaacga agtgcagcac catcacgatt gttttgcctt
    46081 ggtttcatag tgactaattt ttcatttaac cattgaaatc cgtcttcaaa ttctttataa
    46141 gaaaacaaag aagtcttttc tcttgacgaa cggtctgctg tatctccgta aatcaacaat
    46201 gggatatcga ctttcttaga aaattcggca atggcaatcg ctgctttttt tgccgcttcg
    46261 atccgatcat cccgtatcat tgacccagat tcatcgatcc gtacagctac cgctaaagaa
    46321 ggttgctcat gtggcgggtt ctttttgtca aaatttctca aatcaccata tgctactttg
    46381 cttgcattga accgctgacc ttcgtatttt cctttttggt acgtctctgt ttgttcatta
    46441 tccagtaact ctaaaatttg ccggctaagt gattctacta ttggcattac ttgcatgtgt
    46501 agctcacgtg cttccggcgt ttttccttgg aaagctggtc ggtgaacgat gagaccttct
    46561 ttttcatgaa tcgtttcctt catgatttgt cgcgcttctt tattcagttg cttcttgatt
    46621 tccttatcat gtgcttccat gtcagaagca gacatttctt gagaatcatc tcctgtttgt
    46681 tgttcttttt gattttttcc ttcgatatcg ctactgcgtt ctttttgcat ttgaggagga
    46741 atctcgtttt tttctattgt ttcttcttgt ttctcttctc cctcatttgg ttcttcagca
    46801 gacaacgatt tcttttcatc taagcgcaga atttcttcag cattttcagt gatgtttcgt
    46861 tcatttgctt cttcggcgct aatggttctt tcttttgtat gctcagattg aggatgaccc
    46921 catttttctt ttctcaatat gtcctgaagt tccagtagca atccactgtc agacaaagct
    46981 tgttcaagaa ttgccaattc atctgaatca gtggataatt tatacaaaac ttttggataa
    47041 atcgactgga gtaaatcttg tccttgctta taggtatttg cccaataaaa ataagaacgc
    47101 attcctgcta cacctttgat ggcgttagct tttgcagttt catccaacaa acggataatc
    47161 tctgccatct ttaagagaag ttcttgtttt tggatcatag ttttcgccat cgcacgttct
    47221 gccatgactt ctaaggcagg aagatccatt ttttccgcat gttgcatccg atctcttaaa
    47281 gattcattta atgggcgatt tccttggtaa tttcgatttg tagtgatcac aatgacgcaa
    47341 tctggatggc gacgaatgat tcctgtcggt aaattcaaca tcccatttgg ttcgagcgct
    47401 gagttaagtg ccacaagaac agaggcatct cggatgacag tcggctcttg aatctctaat
    47461 aaatagcctt tttcaatcgc tcgaacgatt tcagaagggt aatagctgta atggattgca
    47521 tctggataat cagcttcgat cctttgaact agctctgcta atttttcttt agcagaaaaa
    47581 ggatcaatgc cgtaatggtt ctggatcagt gtcagtacag cttcaagtgt atctgtttgg
    47641 taaatagctt ctagtaattc ttggtcttgt tccttattag aatctactac aggtaataag
    47701 gctccgaaaa catctgattt gtccatatct gcaaaacagg tcacttttgt atagggaagt
    47761 tgcagatcag cagatagtgc tttggctaat tgtgtcttcc ccgacccggc atctccttcc
    47821 aaaagtacgt tactgatttt catttcagga tcttgccaat tccctttgat ttcggaggcg
    47881 atccgccatt cttctgcact tgtccgatgg cttttcggtt tcttccagat catttgcttt
    47941 tctagccgtg aaaaggttcg gttttctgtt agtgagaaat tctccatgct tctcttcccc
    48001 ttttgatcat ctctgtctat taatatccta gctcatctaa aagacgtttt aaaatacgga
    48061 atcgttcccc aatcagttct ccgtcttttt gtagtgtatc gtaatacaaa atgatgtctt
    48121 catcgatctt tggatttttg gtatctactt ctaccgtcat tcggttccct tgtaatccaa
    48181 tgatgtctac gactggattt tctggatcat aaaatttttc ataacgataa gcatcttgga
    48241 agtataaact tccttgcgca gctaagatcg ctaaatctaa aatacgtgtg attggtaatt
    48301 cttccgactg tcgtgaccat ttttctccag tatgtcgcca taccttgcct gagatatcga
    48361 cttttccacg atcattccac tgagcaaggc ccagtgaaag cccttttgca tccatattta
    48421 atgcatttct tccatcaact ttatcatagt cttctacaac gattactggt ttatgtttta
    48481 aatttgttgg tatttccatt attttaactc ctcatccttt tatttttagt aaataagtaa
    48541 attagtagtt tactcattta ctaaactata tgcgacatta tagaggattg aagtgaggat
    48601 tgcaaagaaa aagagcttca ttgatagaaa taaaaacacc atctcgttct tttacacgct
    48661 cattagtaac atgtagaaag tccaagatga tgttggaat
//
LOCUS       DAFCNJ010000017        45295 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000017 DAFCNJ010000000
VERSION     DAFCNJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.017"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1256)
                     /locus_tag="LNE35_001187"
     CDS             complement(<1..1256)
                     /locus_tag="LNE35_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBK6910358.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1269..2486)
                     /locus_tag="LNE35_001188"
     CDS             complement(1269..2486)
                     /locus_tag="LNE35_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBK6910359.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(2476..4071)
                     /locus_tag="LNE35_001189"
     CDS             complement(2476..4071)
                     /locus_tag="LNE35_001189"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBK6910360.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(4581..5363)
                     /locus_tag="LNE35_001190"
     CDS             complement(4581..5363)
                     /locus_tag="LNE35_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910361.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(5428..6264)
                     /locus_tag="LNE35_001191"
     CDS             complement(5428..6264)
                     /locus_tag="LNE35_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6910362.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(6282..7691)
                     /locus_tag="LNE35_001192"
     CDS             complement(6282..7691)
                     /locus_tag="LNE35_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6910363.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(7705..9528)
                     /locus_tag="LNE35_001193"
     CDS             complement(7705..9528)
                     /locus_tag="LNE35_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6910364.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(10371..10697)
                     /locus_tag="LNE35_001194"
     CDS             complement(10371..10697)
                     /locus_tag="LNE35_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910365.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(10743..11105)
                     /locus_tag="LNE35_001195"
     CDS             complement(10743..11105)
                     /locus_tag="LNE35_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBK6910366.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(11142..11357)
                     /locus_tag="LNE35_001196"
     CDS             complement(11142..11357)
                     /locus_tag="LNE35_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6910367.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            11484..11819
                     /locus_tag="LNE35_001197"
     CDS             11484..11819
                     /locus_tag="LNE35_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6910368.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(11895..13892)
                     /locus_tag="LNE35_001198"
     CDS             complement(11895..13892)
                     /locus_tag="LNE35_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910369.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(13990..14265)
                     /locus_tag="LNE35_001199"
     CDS             complement(13990..14265)
                     /locus_tag="LNE35_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6910370.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            14497..15231
                     /locus_tag="LNE35_001200"
     CDS             14497..15231
                     /locus_tag="LNE35_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6910371.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            15270..16412
                     /locus_tag="LNE35_001201"
     CDS             15270..16412
                     /locus_tag="LNE35_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6910372.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(16507..17898)
                     /gene="sufB"
                     /locus_tag="LNE35_001202"
     CDS             complement(16507..17898)
                     /gene="sufB"
                     /locus_tag="LNE35_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBK6910373.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(17903..18373)
                     /locus_tag="LNE35_001203"
     CDS             complement(17903..18373)
                     /locus_tag="LNE35_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBK6910374.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(18360..19595)
                     /locus_tag="LNE35_001204"
     CDS             complement(18360..19595)
                     /locus_tag="LNE35_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK6910375.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(19592..20878)
                     /gene="sufD"
                     /locus_tag="LNE35_001205"
     CDS             complement(19592..20878)
                     /gene="sufD"
                     /locus_tag="LNE35_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBK6910376.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(20891..21664)
                     /gene="sufC"
                     /locus_tag="LNE35_001206"
     CDS             complement(20891..21664)
                     /gene="sufC"
                     /locus_tag="LNE35_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBK6910377.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(21861..22709)
                     /locus_tag="LNE35_001207"
     CDS             complement(21861..22709)
                     /locus_tag="LNE35_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK6910378.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(22733..23419)
                     /locus_tag="LNE35_001208"
     CDS             complement(22733..23419)
                     /locus_tag="LNE35_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910379.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(23412..24446)
                     /locus_tag="LNE35_001209"
     CDS             complement(23412..24446)
                     /locus_tag="LNE35_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6910380.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(24509..24765)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(24841..25179)
                     /locus_tag="LNE35_001210"
     CDS             complement(24841..25179)
                     /locus_tag="LNE35_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBK6910381.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(25181..25537)
                     /locus_tag="LNE35_001211"
     CDS             complement(25181..25537)
                     /locus_tag="LNE35_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBK6910382.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(25543..26730)
                     /locus_tag="LNE35_001212"
     CDS             complement(25543..26730)
                     /locus_tag="LNE35_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK6910383.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(26921..27766)
                     /locus_tag="LNE35_001213"
     CDS             complement(26921..27766)
                     /locus_tag="LNE35_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6910384.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            28000..28965
                     /locus_tag="LNE35_001214"
     CDS             28000..28965
                     /locus_tag="LNE35_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBK6910385.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            29342..30385
                     /locus_tag="LNE35_001215"
     CDS             29342..30385
                     /locus_tag="LNE35_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBK6910386.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(30447..30911)
                     /locus_tag="LNE35_001216"
     CDS             complement(30447..30911)
                     /locus_tag="LNE35_001216"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBK6910387.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            31157..32104
                     /locus_tag="LNE35_001217"
     CDS             31157..32104
                     /locus_tag="LNE35_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6910388.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(32315..34387)
                     /locus_tag="LNE35_001218"
     CDS             complement(32315..34387)
                     /locus_tag="LNE35_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK6910389.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(34320..34697)
                     /locus_tag="LNE35_001219"
     CDS             complement(34320..34697)
                     /locus_tag="LNE35_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBK6910390.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(34826..35404)
                     /locus_tag="LNE35_001220"
     CDS             complement(34826..35404)
                     /locus_tag="LNE35_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6910391.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            35536..36828
                     /locus_tag="LNE35_001221"
     CDS             35536..36828
                     /locus_tag="LNE35_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK6910392.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(36888..38006)
                     /locus_tag="LNE35_001222"
     CDS             complement(36888..38006)
                     /locus_tag="LNE35_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBK6910393.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(38020..39441)
                     /gene="arcD"
                     /locus_tag="LNE35_001223"
     CDS             complement(38020..39441)
                     /gene="arcD"
                     /locus_tag="LNE35_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBK6910394.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(39687..40808)
                     /gene="mnmA"
                     /locus_tag="LNE35_001224"
     CDS             complement(39687..40808)
                     /gene="mnmA"
                     /locus_tag="LNE35_001224"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBK6910395.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            41212..41811
                     /locus_tag="LNE35_001225"
     CDS             41212..41811
                     /locus_tag="LNE35_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBK6910396.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(41949..43235)
                     /gene="efmA"
                     /locus_tag="LNE35_001226"
     CDS             complement(41949..43235)
                     /gene="efmA"
                     /locus_tag="LNE35_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBK6910397.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(43506..43850)
                     /locus_tag="LNE35_001227"
     CDS             complement(43506..43850)
                     /locus_tag="LNE35_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBK6910398.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(43987..45132)
                     /locus_tag="LNE35_001228"
     CDS             complement(43987..45132)
                     /locus_tag="LNE35_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK6910399.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        12994 a         9513 c         7885 g        14903 t
ORIGIN      
        1 actacaaaca cgatatgttg gcccattaat ttttggaagc gattggttcc ttttttctct
       61 tcttgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgattaca
      121 acatttgcat tggcatctgc caacagggtt tcacttaatt ctttggcact tcccgttcca
      181 acgatcaagc tattcgcttt ggcgttcccg gaagaaatcc cagtattaaa ttccgaagca
      241 aattttgtaa actctgtggt ggtttgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgtac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga cgattgtata atccgcaatc aaacgatgag catccgggat atttaacact
      541 tgtttacaaa actcatataa gttttctact ttttcattgt ctttcgttcg ccaactaaat
      601 aaaaacttag ggtgcaagtc tttcggtaag gcatttgcaa aataccgagt ggtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat accttcttct
      721 gcatattttt tgatttggtt aaatgcctga taaacaccat cttttgcagt tacttgtttc
      781 agctcgattt ggacaatcgg taatccattg attaataagg tgacgtcaaa acgtcgatcc
      841 cgtgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcgaatct
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgttcgcag cagtaattcc gttttgatcg tttcaaattc tttatcggtc
     1081 aaaggaaaat cattcagttc tgcttgatta tttgagataa ttttttggcg taaattgacc
     1141 caaaggtctt cttctgattt tagatccgga cgatacgtcc actgattatg cccttctcca
     1201 agtacacgaa tcaactgttc ttcgacttct gcttcctcac ggtgactaat tttcattctt
     1261 cttccctcct agacaaacat cttttgtaag aagctttttt tcgtttcttt tagtaaatct
     1321 aacttacgtt gatgaagagc gatcgtatca tctagctgtt tgaagaaagt accgattttt
     1381 tgttgttcat cctttacagg aaccttaaca ctgatattct caatatcaga actgttataa
     1441 gaaggcaatg cccctacttg ggagtatctt ggcaaattta tagtttcaaa caatatttta
     1501 ccaaaataga tatcccaagt attattaaat aaataagcca tggtattgtt atctattaag
     1561 aaagggcttg tgaccaaacg ttttctatta agcattattg ctgcgccaac tttagcaaat
     1621 ataatcgctg gcacatcttt tattggcttc cattttttac gctcaatttg ctcattagat
     1681 acataatgat ttgcatttct catttcatac tcattaccaa tgttattcat gtcggaaact
     1741 ttaaaaaatg gagtaccttc agaaccacct tgctcggcat caggaaatcc aattcccgat
     1801 tgagttttac ctaattctcc caacttacgc tcttcccaat cttccgtaaa accaggaaac
     1861 cggatttctg gcacttttgc tccgttttca gggaacattt tttgtaagaa tccttttttc
     1921 gtttccttca gcaaatctaa cttacgctga tgaagagcga tcgtgtcgtc aagttgtttg
     1981 aagaaggtgc cgattttttg ttgttcttcc tcactaggaa acattgctac tatattttta
     2041 aactttttta gagacagaac atgttgacct gttccctctg cttgaataga taattgtcgt
     2101 tggattccat tgttaatacg tcttaagaag aagtcggaat cagcagattt taaagtaaat
     2161 acaggataaa aatagcttat aatccctcta tcaataatgt cattacggtt aaacacaaac
     2221 acatcattat cacttcgact acggaacgtg aaatatcctc taggtagtac aaagtaacca
     2281 atattatttt ctgtagttac ttgccgattt gcaaaatagt cttcttgcaa aactatccct
     2341 ttttgttgag atgaagttag cataggaaat tgattttgta cacttgtctt ttcaacataa
     2401 tctacaacga agtcccccaa cttcctctgt tcccaatcat ctgtaaatcc tgggaaacgg
     2461 atttctggtt gtgtatcatt gctcatgttt gaacacctcc agcgcacctt gtaaccacgc
     2521 ttcattttca ggtgttgttt gcagggaaga gatagcttct aaaagttttt tttctagcac
     2581 ttgctgttct tgtcgtatct tcttgatgtc atttccgaca tgaaccatat caattggttc
     2641 ttcttcttcg aaagtatcta catagcgtgg gatatttaag ttatagtcat tttctttgat
     2701 ttcatcaaag gttgccaaat gagcgtattt ctcgacatct tttctttcag cgtaggtttc
     2761 gaggattttt tgaatgtttt cttccgatag tttattttga tttttccctt taacaaattc
     2821 acgactagca tcaatgaata atacatctcg agtttgacga ttcttcttca acacaatcac
     2881 cgtcgttgga atcgatgtcc caaagaacaa gttcgcaggc ataccgatca cagcataaat
     2941 actgccatct tctagtaatt tttgacggat gaccccttct gcagcgccac ggaataaaac
     3001 tccgtgtggt aagacgatgg ccatggtacc tgtttccttc aaatgataga acccatgaag
     3061 aagaaacgca aaatctgctt tggattttgg cgctaatttc ccataacgat taaaccgaga
     3121 atcatctaag aaagtcgtat ctgctgacca attagccgaa taaggtgggt tcattactac
     3181 tgcatcaaat gtgtaaggtt catctgttgg ccagtcctta ttcaacgtat ccccattgcg
     3241 gagattcatt tcttcggcgt cgacaccatg taaaatcaag ttcatttttg ctagattgta
     3301 tgtcgttgta tttagttctt gcccatgata tttaacgtta tctgggtgtg ttaagtagtt
     3361 acgtacgttt aacatcaatg aacctgaacc catcgttgga tcaaaaacac taaagaatcg
     3421 tcgttccttt tgatcgagcg ttacgatttg cgccatcata tcagaaacca tatgaggtgt
     3481 gtaaaattct ccggctttct tccctgcttc tgaagcaaat tgactgatca gaaactcata
     3541 cgcatccccg atcacgtccc catcgtgttc taaaacatct acatcattca atttcttgat
     3601 gacttcggta atcgtcacat ttcgttgttg ctcatctgtt cctagttttt ttgattggag
     3661 atccacatca tcaaataacc cattaaattg attgtaggta gatgccaatt gcacaaaggc
     3721 tttgtttaga tcgttcaatt ggaacgttgc ttgttttgct tgatccgcta agacgttgaa
     3781 aagatactct ggtgcaattg catatcccaa aatatcaacg attgtggcaa tcagatcttc
     3841 ttttgattct tcatctgaaa gtaattcacg atacaacatt gtttgtttgc ttactgtgtc
     3901 atattcctct aaggattcat ctgctaaaag aaccacttgt tccaacaatc gatcggataa
     3961 atatttatag aaaatcagtc ccaataaata atttttatat tctgacgcat ccattttact
     4021 acggagatta tctgccgcac taaataattt ttgatttaat tctgcaccca aaaagataca
     4081 ctcctatctc ttatttaaag ttgctatata ccctcttatt ttagcatacc cccttttata
     4141 aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg aaaggagtct
     4201 aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt ctacatagtt
     4261 atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt cataaaaaaa
     4321 cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca ctaaaatcac
     4381 tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc tccgtttcat
     4441 ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt
     4501 atcctaagct actggagatg tataagtacg gtcatataac aacggggtag ggtaaataaa
     4561 tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt
     4621 acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag taaccgaagt
     4681 ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag attagctaat
     4741 aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct
     4801 ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc gatgaatgaa
     4861 tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact cttagatgaa
     4921 tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt ctcaccggaa
     4981 actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt taaaattccc
     5041 acgcttttac taataaattc actataacca gttctttcaa tagagacttc aatgtatttc
     5101 acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt tacatctaca
     5161 taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt actgtctgat
     5221 atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc ttctggcaag
     5281 ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac attcacctcc
     5341 gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac aagtctttat
     5401 atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca gtgttagata
     5461 aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact ccttgatttt
     5521 caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca aaaaatcatc
     5581 cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta aatgcgtcac
     5641 aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca gatatcctag
     5701 catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca tatttgcttg
     5761 ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt ctggcaactg
     5821 aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa attcttttgg
     5881 gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt tttgactgcg
     5941 ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat tcagcttaaa
     6001 gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta gatcatccgg
     6061 agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg aatctaattt
     6121 aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt gaaatccaat
     6181 ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa ttaaactgtt
     6241 gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa gccatttctt
     6301 gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa ttctttaaga
     6361 ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc ttgatgggta
     6421 ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc atctgttaaa
     6481 gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg gtcattaatt
     6541 cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat taaaataggt
     6601 aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg gtcaactacc
     6661 cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac accttcttct
     6721 cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc atttttgctg
     6781 gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt accggaggat
     6841 aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt caaatagttt
     6901 tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca gcctctcaat
     6961 gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg atacatagcc
     7021 gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata caagtgaatt
     7081 actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt ttttgaaggc
     7141 agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt gttttgctca
     7201 ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac ctgtacatag
     7261 tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc tagaaaataa
     7321 ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag gcattcatca
     7381 attacttgat gataaaaaga aattcccttt tgattggttt tattattttc aatatccata
     7441 attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt aaacagctgt
     7501 atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc taccccttct
     7561 gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata ggcaccctct
     7621 gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc tgaaagctgt
     7681 ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt gttcctttaa
     7741 gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta ccacaatcac
     7801 tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta ataattgtcc
     7861 tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt ttaaattcac
     7921 ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta gaccaatcgc
     7981 atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa tgctgttttc
     8041 ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag catcgttcac
     8101 ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg cttcggctat
     8161 tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag caatgataaa
     8221 ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca ttggattttc
     8281 tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc tgactaaacc
     8341 gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa ctaatggttt
     8401 tttcagcttc aaggttactc catagagtga aggctcagta attcccatta aagcagtgat
     8461 cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg ctacagcaaa
     8521 ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga tcatttcaga
     8581 acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg tacccgtaac
     8641 caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg ctttgccgtt
     8701 aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg gtccaatgac
     8761 aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa agaatttaat
     8821 aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc aaacagtaaa
     8881 gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc ctagaaaaga
     8941 aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa tgccagcaac
     9001 agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg ctaatagtac
     9061 tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag tttgactact
     9121 gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca tcccggcacc
     9181 tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca atcttgaaat
     9241 aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca cttccttttg
     9301 aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt gtccaccgct
     9361 atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg ctttcctttt
     9421 atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca cattttcttt
     9481 tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta ttctctctcc
     9541 ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa gtgttagttt
     9601 gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc tgtaatccaa
     9661 taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct tttcgtttga
     9721 ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc gtatacttac
     9781 tgctaaaata agtatatcta atattaacta taccagtaaa accattccta cataaccagc
     9841 gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac acaacttcaa
     9901 caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa taaacagcat
     9961 tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca gtaagcaata
    10021 gccaatttca atcattccga gatataaagg tatcgataga atgatttggg ctagtattct
    10081 taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt agtaagtatt
    10141 tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt tttctttttc
    10201 atttctttta tattgttata acagtgaata acataaaaat tcccgacaat cattgtaata
    10261 tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact ccctagtgaa
    10321 tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa tcaatttaac
    10381 attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg atagtactca
    10441 atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta tgtgttatct
    10501 ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta ctgttcttgt
    10561 aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc tgtcattttt
    10621 gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa cttcttgttc
    10681 atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc ttatccctct
    10741 tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac ccctgctatc
    10801 atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat aataacagca
    10861 acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat ccaaccaata
    10921 atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt
    10981 gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtagt
    11041 gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca ttttctacaa
    11101 tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt tactttatta
    11161 aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag catcttcatt
    11221 tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca
    11281 ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta tcggctgaga
    11341 ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat
    11401 ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa cttgttgtac
    11461 tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc attagagaaa
    11521 agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc tctacacaac
    11581 aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc ttaacagctc
    11641 tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc gtagattatg
    11701 aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt cgaacaattg
    11761 actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg aaagattgaa
    11821 aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa gattaagagc
    11881 tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac tctccgtcca
    11941 tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc tgataatatc
    12001 aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa aaaactacta
    12061 cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag agtgaatcct
    12121 tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt atccccaaca
    12181 ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag atatgtccct
    12241 tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg tttttccttg
    12301 ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc gttaaaacat
    12361 atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc cagaagtgga
    12421 aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc taatcgatag
    12481 acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa cctatttagg
    12541 tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa tcctataaaa
    12601 gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc atagtaagaa
    12661 ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc cttactatca
    12721 tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc ctctagataa
    12781 ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa aagtactttt
    12841 gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc taatagccaa
    12901 ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc aataaaattt
    12961 tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt tttactggta
    13021 aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt tttacttgac
    13081 ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt aaaagacatg
    13141 tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt gtcgtcattt
    13201 tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc tgaatcactt
    13261 tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat atctaacatg
    13321 ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa cattctattc
    13381 gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc gtaaacagca
    13441 accattttac catagtcagt aattgcttct tttaatagaa agacaatctt gtatttttct
    13501 agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg aactaaattg
    13561 atagataaaa ccttttctac gcttaagtat ctactatcat ctttactcaa atctactacc
    13621 ttccccttct catctttaac agtgttatcg acatccagaa taattactct ggtttccaca
    13681 aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga ttgtgatgaa
    13741 tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa acacctatct
    13801 cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc ttgatctaca
    13861 taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata aaattctgtt
    13921 aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt tttagtctta
    13981 tttgtttact caacctttat accgttccat gaaactatca atatcagatt ttttaaatct
    14041 aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt ttctaatcgt
    14101 tcctactgat acacctagat attcacttgc ctgctgatag tccatatact ctagagtgac
    14161 tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt tcacttgttg
    14221 ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc cccctccttt
    14281 cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc aaaataaaag
    14341 tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc gacatacaca
    14401 taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttgc aacacccctt
    14461 ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc accaataaat
    14521 tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa gagtaaaaaa
    14581 ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga caaaaaaact
    14641 atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc ccgaattgct
    14701 gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga atatattttg
    14761 ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc agataaaaac
    14821 ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga tacatggctg
    14881 gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa gacttgtaat
    14941 aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga attattcatc
    15001 aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag tatagaagaa
    15061 ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat gtcttatccc
    15121 gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac gatttcatca
    15181 attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta gtggtaagta
    15241 ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac aaaaagaaaa
    15301 acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga actggtgaaa
    15361 aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa gattatgacg
    15421 agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg acatttgaag
    15481 acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca acaatcgcaa
    15541 tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt aaactatcga
    15601 atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa tatgaaagtt
    15661 acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca atgcaatata
    15721 ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat gagaaaaatc
    15781 gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa gattttggta
    15841 acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga aagagtgaag
    15901 tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat gtcaataaaa
    15961 ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca agagcttcta
    16021 gacgagttat ctctttagat actgagactc tttctatcct caacaactgg aaattacagc
    16081 aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa catgttttta
    16141 caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta agatatattt
    16201 tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca catgctagtt
    16261 tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg catgaaaata
    16321 gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag aaaacaggtc
    16381 aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct ggtcatttct
    16441 agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct agctcctata
    16501 acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga gttcgactgc
    16561 atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga tcatttcagt
    16621 tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt ctgagatttt
    16681 tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat tgaatgggat
    16741 cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat gagagatcga
    16801 acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc cgccgtcttt
    16861 tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga tcatttttgc
    16921 acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg tgcctcgtgc
    16981 accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac ctaggtttcc
    17041 gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta ctaagttgta
    17101 cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt ttcttgtaaa
    17161 gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg tacaaccttc
    17221 gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga attgccccgt
    17281 gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga cgccttttgg
    17341 cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta atttgttgtc
    17401 ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt ctttcaaagc
    17461 agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca tattatggta
    17521 cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt cagcttctgg
    17581 tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc agtcacgagc
    17641 gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt ctgaaaggtc
    17701 ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca gacggaattc
    17761 aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt cttctgtcaa
    17821 gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt tatattcttc
    17881 taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg aaagctgcgg
    17941 taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca catttgatcc
    18001 gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg tcgggatctt
    18061 tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct gcttcttcta
    18121 ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg atggagcagc
    18181 cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact tgcaattcga
    18241 tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca gtcaagcttc
    18301 cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa ttgtctaatt
    18361 tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac taaacggtcg
    18421 gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga tacttcaagg
    18481 tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc ttccatgtct
    18541 aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac gactcctgtc
    18601 cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg taattttggc
    18661 aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc gagtttttgc
    18721 aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt ccctgcttcg
    18781 aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc gatcatttcg
    18841 ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata taaaacacca
    18901 atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca gtctagtgct
    18961 tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat gaccccgccg
    19021 ttttcatgga caagttcagc taattcttga acaggattga agacacctaa gacattggat
    19081 acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc atcctccata
    19141 tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt tcgttttgcc
    19201 aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac tatctcgtct
    19261 cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct tgtcgtcgta
    19321 ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg tactttttct
    19381 cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc gcgatgaaca
    19441 ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt tggcttttgg
    19501 gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg aaataagata
    19561 ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct tcgattactt
    19621 cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa ccgaggaatc
    19681 ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc agatagtaca
    19741 tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact tcgttttcat
    19801 cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc aagacacgac
    19861 tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata ccattaaagg
    19921 tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc gtatgagggg
    19981 ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg attgccacga
    20041 ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca gcgatcacat
    20101 gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa atgtgcccac
    20161 ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa tatttgattt
    20221 ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt gctacttgtt
    20281 ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct gccacgatca
    20341 acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag gactcgagtg
    20401 gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc ataaaagcta
    20461 catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata taatattctt
    20521 ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg actccttttt
    20581 ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg acaagcacag
    20641 gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg gtttgtgttt
    20701 ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc ggcaatggca
    20761 gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca ggttcgcctt
    20821 tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt tctttcattc
    20881 ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta ctgataccag
    20941 cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg actactcggc
    21001 cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt tgatagtggg
    21061 tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta gcaacgactt
    21121 tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat gtaggttcta
    21181 acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa ccttcgttca
    21241 gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca tctaactttt
    21301 tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg atcgctgcac
    21361 gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata gctaaaaata
    21421 agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg tcaaacaaaa
    21481 tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat aaggtagatt
    21541 ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt gttaggttta
    21601 cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt tctaagacag
    21661 acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta attgagaatg
    21721 aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta aacaattttg
    21781 atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag acttatttct
    21841 catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac agagccgccc
    21901 catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat cagttttttg
    21961 attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta aggagagttg
    22021 tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta agcgaagttt
    22081 gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa aagtgatggg
    22141 tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc gaatgttgct
    22201 gtttccagat ctactccatc tttgactttg actaagccag catcttgtag aattgtgatg
    22261 atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc ttttaattcg
    22321 gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac agcaccggca
    22381 ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat atgctggaag
    22441 taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata gtcatcgaat
    22501 ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg ttccaaaatc
    22561 tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc tttatttgct
    22621 gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag caaaagcgct
    22681 gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc tattatcttt
    22741 tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg attagtaaaa
    22801 taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct tcctgccaag
    22861 caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat ccgatcatcg
    22921 agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt atcaagacct
    22981 tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt acaccagggc
    23041 tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg gataaaacaa
    23101 gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta gtaaatggaa
    23161 tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg atgatcgata
    23221 cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag taaaggatca
    23281 atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc atatatagtg
    23341 tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct agatatgttt
    23401 ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc tttgatgaac
    23461 acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa tgaaccaaag
    23521 gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat gttatagcgt
    23581 cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt caaagttgcc
    23641 aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga atcagcttct
    23701 ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg tctgaagaca
    23761 gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac tttgttgcag
    23821 attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt caatttctta
    23881 ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa tgcgctagta
    23941 gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat accgacgcgt
    24001 tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc taacccgact
    24061 aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc agctaattcc
    24121 agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa atgctggaag
    24181 atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt tgtaatatct
    24241 tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag gagacggact
    24301 aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc tttttcaacg
    24361 tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa tgtctttttg
    24421 acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttatga tttttcaaac
    24481 aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact tccgtgttac
    24541 caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga cttagcttct
    24601 ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta cgctcgctta
    24661 ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca aacagggctc
    24721 tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc atataggatt
    24781 gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga ttttctgatt
    24841 ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc acgcattcat
    24901 ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa cagaagaaac
    24961 tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa cttcgatcag
    25021 aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg tgatgtctcc
    25081 aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat taaataagat
    25141 ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt tctccttcat
    25201 aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc agtggtcgct
    25261 tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta aactcgccta
    25321 cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac aggcgacgga
    25381 ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt acttctttga
    25441 tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg gcttttttgc
    25501 atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc ttgaagatct
    25561 tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga tcatgttccc
    25621 taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta aaggaagtaa
    25681 tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga tcaaaccgct
    25741 tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca tacgatagat
    25801 taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt tttcaccgat
    25861 aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta cgttgaatcc
    25921 tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct gataactcat
    25981 tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact gataagattt
    26041 gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga agatcgttcc
    26101 tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag agattccaga
    26161 catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa agtcatcttg
    26221 ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa tcagccaaaa
    26281 gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga tcagtgccat
    26341 catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg caccgaatcg
    26401 aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt tgagcaagag
    26461 tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga tccatttgga
    26521 attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga caacttgcat
    26581 ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta catataatga
    26641 gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat caatacgatt
    26701 atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa aaccatactg
    26761 ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca tttgggaaaa
    26821 attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct actgcttatc
    26881 tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg agattctcct
    26941 cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca atctctagca
    27001 gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg aaattcaacg
    27061 gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga ccgattcctt
    27121 ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta tcttggatat
    27181 tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct tctcttacaa
    27241 gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc aaagagtatc
    27301 tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat tcatgtttcg
    27361 ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc actttaggaa
    27421 accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag gtgtgggaag
    27481 caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc tgagtttctt
    27541 cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc ttagttggaa
    27601 gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc tccaactgtt
    27661 tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct ctggaaaaat
    27721 ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca ccacttccat
    27781 aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa aaccatctat
    27841 aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg aagacaatca
    27901 gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt cgttcatctt
    27961 ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt tttgaaaatt
    28021 attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa tctgtttaaa
    28081 gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat tcttatgata
    28141 ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt gcatgatcca
    28201 atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg tacttatttt
    28261 gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc tgttatcttc
    28321 caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga tctttcaatt
    28381 gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat cactgtaact
    28441 gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt gatctgctat
    28501 gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat ggctcatccg
    28561 acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac aggttatctt
    28621 aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt atctcaagta
    28681 ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt ttatccaagc
    28741 tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac tactgttcat
    28801 tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt tgcggaatgt
    28861 ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt cttaattaaa
    28921 gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc gattcaatag
    28981 gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa cagctgttca
    29041 tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaccaa
    29101 gaggtcgtaa aagaagtggt cagcttcaag aaataagaag gaatttacga aaattgcttc
    29161 tcaaattttt gtgaaatttc ggcttatttc cgtagaagct acttctggaa caccgtttat
    29221 tcgaaacaag aggtcgtaca agacttttgt cacgacctct tgttcttttg tcaaaatact
    29281 ggtttagtgc tactatgttc gagtatgctt ttagtattta ttgacgaaag gaaccgatca
    29341 aatgaaatca ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc gcgaaatctt
    29401 acccggttta ctgctttctt ttgccatcgc tgccttcagc aggcttcttg ctatttatct
    29461 gccacagtta ggtggagcga ctattgctat tttattgggt attttattag gaaatacttt
    29521 ttttaaacag cctgttctag caatcggcac aaaatttgct gaaagcaaac tattagaatt
    29581 ctctgttttt ttactaggtg caactgtcac agctcaaacg attgctcaaa ttggtgtaaa
    29641 aggaattgtt tttgtgttac tgcaaatgtc agtcactatc atcggtgctt acttgattgg
    29701 taaaaaactg caattcagcg atacttcttc tttgatgatg gctggaggaa acgctgtttg
    29761 tggttcttct gctattgctt caatcgcccc ttccatccat gcgaaagagg aagaaaaagg
    29821 acagatcatc acactagtca atctactggg tactgtgatg atgttgactc tcccttttct
    29881 tggattacag ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg gtggtacact
    29941 ccaatctgtt ggtcaagtag tcgctggggc aagcttactt gacaattctg ttgtcgaatt
    30001 cgccatgcta tttaagattc tacgtattat gatgctcgtt gttgttgtct atctgtttga
    30061 aaaattcatg cacaagaaac aaccagctct gtttgaaacg ccaactgata cgcagaaaaa
    30121 acgaaaatta ccgcttcctt ggtatgtatt agggtttgtg atcttttgtt tggtcaacag
    30181 cttgttttct ttacctgtag ttattgacca atcggcacat tttatcagca gctggtttga
    30241 gatcactgct ttagcggcaa ttggtctgcg tttagacttt gcaaaattca tgaaagaagg
    30301 tacacgcttc ttatcttatg gattagctgt cgggcttctc caaacagtga tggctttact
    30361 atttatttgg ctgatcaaag tttagaatga acatgaaaaa acagttgtga ttctcgcatg
    30421 gaacaatgtc cgaatcacaa ctgtttttaa ttttgcagat atccttctaa caacaacaat
    30481 tggcgtttga tttcaacccc atctattgcg tttcccatat atcctgtcag ttttccattt
    30541 tgcccgatca ctcgatgaca aggaataacg attggcaatg gattggttcg atttgcctgc
    30601 ccaatcgctc gcacagcttt cggactatca acagcaacgg ctacttcttt atacgttcgg
    30661 atttctccat aaggaatggt cgacaaagct ttccagacat tttgctgaaa aggcgtacct
    30721 ctctttatag ataaaggaac atcaaattct tttctttttc ccgcaaaata ttcttcaaac
    30781 tgttcttgtg tgcgcgttaa aattcgctga ttcgctgatt cgctttctga aattggctga
    30841 aaagatacag cagtcaatcc ggattcatct gcatctaacc ataaaaagcc gataggtgtg
    30901 tgtattttca ttttgtttgc tcctagtcaa tagactggaa cttcctcctt tttctctatc
    30961 caaaactact tgccaactaa taaaaaatgt tcttaaaagt ttgataaacc gaggaaacat
    31021 ggtaaactaa atacgttagt ttacaaaaag aatacctgtt cttttacgta aaatcaaatt
    31081 ttaaaggtgt tatttaaggt ttattaaatt ttgaatgtaa accatatata aacatacgat
    31141 tgaaaggata gaaacgatga aagtatcaat tgttgtccca tgttttaatg aagaaaaaac
    31201 tgttcctctt ttttttgatg aggttgaaaa attcaaaggt gcccatgagt tcgaatattt
    31261 gtttattgac gacggatcaa aagacggtac gttagcagca atcaagaagc tagcctcagc
    31321 ttatccttat gtccgctttg tctcattttc cagaaatttt gggaaagaag cggctctgta
    31381 cgctggattg caagcttcta caggagattt agtgaccgtg atggatgttg atctgcaaga
    31441 tccgcccgaa ctgcttcctg agatgatcaa ccggatcact acttctgata tcgattgtgt
    31501 cggcacccgg cgttcgactc gtgaaggcga gcctgcgata cgcagtttct ttgcgaaaaa
    31561 attctaccag ttgatcaata aaatcagcga tacagaaatg gtcgatggcg ctcgtgattt
    31621 ccgcttgatg actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat acaaccgatt
    31681 ttcaaaagga ttatttagct gggtcggatt taaaacagaa tatatttctt atgaaaaccg
    31741 cgaacgtgtg gctggcgaaa catcttggtc cttctggaaa ttattcaact actcgattga
    31801 tggtatcgtg aacttctcgg atgcaccgtt aacgattgct tcatttatcg gcgctttgtc
    31861 ttgtgtcggt tctggtattg caattttgtt cattattttg cgggcattgt tgtttggtga
    31921 tcctacatca ggatggcctt ccatggtctc gatcttcctg ttcattggtg gaatccaatt
    31981 gttatgtctt ggaatcatcg ggaaatatat tgggaaaatc ttcttagaaa cgaaacagcg
    32041 tcctgtttat atcataagag aaacagaaaa agtgaagcca aatgaattga agcctcacca
    32101 ataatatacg tcaaatgaag taaacatttt tttaaatgaa aaaggactgt gacaaaagtc
    32161 ttgtcacagt ccttttttcc gaataaatgg tgttccagaa gtagcttctt cggaaataag
    32221 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    32281 tgaccacttc tatcacaacc tctttcatat cttattactt cttcattgct gcttttagtg
    32341 ctgctgctaa tgggctttct tctggttctt ctgcttgtgc atattttttc agcaagcgtc
    32401 gttcttcatg tttggtcatt ttcttcttct gctctttttt atcaagcaat ttttctgtcg
    32461 ttccatcgta tttacaacgg aaataagcac cgtttttacc ttcgatgatt tccattttcc
    32521 ggtgacattg cgggcatcga tgattagaga cttttggatc ttttcttctg cggtagttgc
    32581 aatctgaatt ggtgcagaca tagattttgc cgtcacgtgt gtttttctcg cgcagattgg
    32641 ccccacattc tgggcatttc ttttgggtaa tcgagaaatc ttgatattct tgtttgcttg
    32701 atttgatctc attcaccaag cgttttgttt ctttttcgat tccattcaag aaggttcctg
    32761 cttgtttctt tcctgcagca atagcttcaa ggtctttttc ccagctttct gtcaaatctg
    32821 gtgttactaa agagggattt accaatacta ataattgctt acctttagga gatacttgca
    32881 gtccttgagg ggtacgttcc atcagttcag atttgattag tttctcgata atctctgcac
    32941 gcgtagctgg ggttcccaga ctgtattttt ccatttgacc aagcaagctt gcttctgaca
    33001 atggtttagg cggtgcagat agttcttttt tgatggcgaa atcgggattg attctcattc
    33061 ctttttccca tttgactgtt tccgtctcag tttcagtttc gcctttccat cctgcttgga
    33121 cgacacgatt ttgccgaaaa atgaatcgtt ctttaccaaa ttcaaccgta acttttgttt
    33181 ggctgacttt atggggttga gcaaacagtc ctaaaaaacg agagacaatc atttgataga
    33241 tttttcgctc ttcattactt aatttttcat aacgtgctct gttttcagtt ggcaaaagtg
    33301 catggtgatc ggtcacttta ctgtcttgga atacttttgt ctgttggacg atggctccgt
    33361 ttttcaaata ctgcttgacc tcaggagcaa agtcgctgac tgcttgcaat cgttctttca
    33421 tcgtactttt catgtcattt gtcagatatt tgctgtctgt acgcggataa gtaacgatct
    33481 tatgggtctc atacaaactt tgtacaagac ttaatgtttt ctttgcagaa tagccatagc
    33541 gttgattggc ttctctttgg atctctgtta aatcataagg caatggcgca gattccgttt
    33601 ttaatttttc ttggacttct gtgacagttc ctttgcttgt actgatcaat tgaacaagtt
    33661 tttgtgcttc ttcttgtgtt tttaaagcaa attgattttt ttgaagcatt ttggcttgtt
    33721 cttttccagc ttgcaacgtg atcgtgaaat acgtttgtgg acggaactgc tcgattttct
    33781 tttcttggtc tctcactaat gctagtgtcg gtgtctgcac acggcctgct gataaattat
    33841 ccgcgtactt cacagttaag gctctggtca cattcaatcc taccagccag tccgcttttg
    33901 cccgggcaag agcagattca tacaatgcat catattcttt cgcaggtctt agttgcttga
    33961 atccagtttt gattgcttta tctgtttgag aagagatcca taaccgcttc actggtttat
    34021 tgaaatgcac atactctaag atccagcgag caacaagttc cccttctctt ccggcatctg
    34081 tcgcaatgac tgcttctgag acatcttttc gctgagccag ctgcttgatt gcttttaatt
    34141 gatgtcctgt tttaggaagt ggtttgattc ctagtttttt agggatcatt ggcaacgtgt
    34201 ccatttgcca gctctgccat tccttattca gatcttccgg cattttcaat cctaataaat
    34261 gaccaagggc ccaagtaacg atcaccttgg gtccttcata ataatttttg tgcttttgat
    34321 tagcaccaag caccttgctt aggtctttcg caacgcttgg tttttccgct agaattagtt
    34381 gtttcataaa attcctttct gctttctact gtgatgggct gatgattttt caacaacgca
    34441 agtcccttgt attgctgtaa atcatcgtca cactcttcac accagcgctc atctccgtca
    34501 ttccaaaagg cgctgagaaa gttacttttt gcattgatat attcataatt ttgatggatt
    34561 tcgttccatt gtttttcgta cgcttgtcgt gcttcttcga aagactcgaa tgtttcttct
    34621 tcttcaatat cttcttccca gtcttcaaaa aaccaccaag gctcattgtc cccgaacatg
    34681 gtaattactt gatacatact cgtgctcctt ctttcagctt ttctagactc atcttagtca
    34741 cttttcaaga aagttgcaag aattttctct tataaaaggg tcatttaatg aacatcttaa
    34801 aattgttaca aatgaatctg attgtttaag gcaaagtagc gataaagtta ttgatcatcc
    34861 aaaaatagct gtcttctgga tcgattgttg gttcttttgt aaagtatcct aaaaaacgta
    34921 aggaaagata accgtggatc atactacgaa gttccctgct tcgatgaact ttttcttctt
    34981 tcgttaattg aaaagcctca aatatctgca aaatgatctg attcgtttca tggattccat
    35041 ctaataaaac agcttcattg gtatgcggta cactgatcag taactcatac actgcttgat
    35101 ttgaaaaagc aaaggatcga taagtttctg catagatctg cagtgcttcc tgaccacttt
    35161 ttcccacaag agcccgtcgc agctggtcat taagttcctg cagaagtttc gctgttagcg
    35221 ctgttttgac atcacgaata tttttgaaat ggttatacag cgagggagcc ttgatatcca
    35281 agcgtttggc taaatgctga aaggttattt gctgaacatc catctcttct gctagttctc
    35341 gaaagcagtc gatgattttc tcttgagaca gatttctttt tttcatttcc atgtttcctc
    35401 tcataaaata aatccttcac tctttcagtt taccacgaaa aagaaagaga aaaaagagac
    35461 tttcattgat aaactacata ttgtagttta taataaagag ctgtagatga aaattttcag
    35521 aaagaaggag tctaattggc tgcaagaata aaggaaaaat tatttaggaa aagagaaact
    35581 tgggaacaaa acttgtttgt cttatggttt ggaacattta tggctggtat cggatttagt
    35641 ttagtcatgc cttttatgtc actttacata gatacgttag gtaattatag tacgtcacaa
    35701 ttgaactttt ggagcggatt gactttttca tctactttct tggtgactac gcttatctct
    35761 ccttggtggg gaaaattagc cgaccaaaaa ggaagaaaat tgatgctgtt gcgtgcttca
    35821 ttaggaatgg caatcgtcat ttctttgatg ggttgcgtga ctagtgtgta tcaattagtc
    35881 gcattgcgtc tgcttcaagg gattttttct ggttatatca gtaatgccac tgcccttgtc
    35941 gcaactggga cgccaaaaga aaaaagtggt caagttcttg gaaccttagc aactggttct
    36001 gttacaggaa cattacttgg accgcttctt ggcgggatct ctgcttcagc gtttggctat
    36061 cgccctacct ttttcattac agggatcatc ttatttttcg tcttcttgtt gagtctgttt
    36121 ttcgtccatg aaaaatttgt cccagtgaaa aaagaaaaca tggtttctgc caaacagatt
    36181 ttcaaagact tgaaatatcc acacgtcgtc atcggtatgt tcgtcacaac gatgatcatc
    36241 caagcatcca acaattccat tagtccaatc atcagtctgt acattcgcca attgctgcat
    36301 ggacatggga atgttacgtt gatcagcgga atcattgctt cgatacctgg gattgccact
    36361 ttgatagccg ctccaagatt tggtcgctta ggtgacaaga ttgggagtga aagaatactt
    36421 gcgattggtt tgggttttgc gattctggtt tatatcccaa tggcattcgt cacgaatgtt
    36481 tggcaattag ctgccttacg ttttttgatt ggtatctctg atgcttgtct gctccctgct
    36541 gtccaagcat tgatcacaaa atattcgcct catcaagctg ctggacggat cttcagttat
    36601 aaccaatctt tccaagcaac tggtaacgta gtagggccta tgatcggctc cagtgtttcc
    36661 agtgcatttg gttatcgtgg cgtctttctt tcgacttctc ttcttgtttt ggtcaacttc
    36721 ttgcttgtac gacacaatac acaagagatc catcagcaag aaactgcaga agaaaaagct
    36781 tcaggagtct ccatagcttc agggcactct ccttctcata gacaataagc gtaaatgaaa
    36841 aacactgctt gaacagttgt cacagactgt ccttgcagtg tttttgttta ggcctgtttt
    36901 ttcaagaact cagataagag gaataatccc ttttccaagg tttctttttt acaacagtaa
    36961 cctagtcggg catgcttcgg taaatcaaag gcacttcctg gtactaatag tactcctgtt
    37021 tcttctaata aattgataca aaatgtctgg tcatcttctt cgatggctaa tttaatgaaa
    37081 gaagtagaga cgtaattggg agcgacaagt tcaactttcg gttcattggc tacccattgt
    37141 tttaaaatca caaggttctc tgtaatgatc tttttgtttc gcgccaatat tttttcttta
    37201 ttcttcaatg catgtacagc aagatcatct gacaggactc cgccgcaaat catcgtgtag
    37261 tctcgatact ttcgaaaaac ttctgccaac tcaggtccag tagctgtcca gccaatacga
    37321 atacctggaa tggagtaggt tttggatagg gaattggttg ctataccttt ttcatagaca
    37381 tctacgatcg acaagaattc acctttatcc gttaaaggcg catatacttc atctatcaat
    37441 acatatgcat ctactgtacg agcaatttct gcgatttttt ctaaagtttt ccgatctagc
    37501 aatgtgcctg taggattgtt tgcactattc aaacagatga tttttgtttc aggtttgact
    37561 aaggcttcta gctgttctaa atcaaactgc caatcttctt cttccttcaa atgaacaaag
    37621 tcaacagcgg cacctaaaga tttggggatg tcatataatt gctgataagt aggaagcatc
    37681 gaaacaacat ggtctcctgc ttccactaac gcgtatagag ctaaaagatt agcaccagtc
    37741 gctccattcg tttgtaagat gttttccgga tcgacggttt gataaagaga agcgacttct
    37801 tgtttgaaaa catctgatcc ttcgatccag ccatagtcca atggttcgtt tttgcgtttt
    37861 tcgaaaaatt tacttacgct agtcccatct aagccaacga gttcgtctaa tgtcaatgct
    37921 tcgatcgagc tttgtgcaat atcatatacc gctttctttt cccatttatt tagccattct
    37981 tctacaccga aatttgcgat tttcatgttt ctttccccct tatatcttga tccagcctac
    38041 agcaagcaac ccgattgaaa gcagtgcggc cacacaaatc agcccaatca ttactttttc
    38101 ttttcccgta atttgatgtt tggtttcacg acaagcgatc cagtagaagc aaaaaccagg
    38161 aagataggca atactacata aaaggacata agaaaatcct gctaaaatga tgcccatcaa
    38221 ttcaaaaact acagcaatcc caccgatcac tacttgtttt ttgattcctt cttttaaaga
    38281 aaactggact tgatagatcc cgacaagtag ataacaaatc aaaattgccg ctgtacataa
    38341 agaataggca aattgatagg cataatcagt aactaagaaa gtcaataaga atagattagt
    38401 caagattgca gtaacaacta acgaataagt tggggcattt tttttattcg tttttcccca
    38461 acctttcggc aacagaccgt ctcgagccat caacatcgtt gtttctgctg ggagcatcgt
    38521 ccaagacaac caagaaccaa tgatcgaaat aatcaagccg atattgatca gcgctgctcc
    38581 ccaatttccc acgattcctt ttaaaatatt tgccatagca ggttgtgaaa tagttgctaa
    38641 ctcttcttga gataagacgc cgtatggcaa gatcgaagca aggatataga taaacaataa
    38701 acaaagcaga ccaatgatcg atgcttgctg ggcttctgaa cgtttcttcg cgcggttggc
    38761 taaaacactt gcgccttcga tccctacaaa gacccacatc ataaccatca aacaaccttt
    38821 aatctgcaac cagatcgttc cttgctgccc tgttccattc actagattat cagatacgtt
    38881 accccaaaaa tcagcagtga atatattcac tttaaatgcg ataaatacaa atattaaaaa
    38941 taggaataat ggcactaatt tacagatcgt cacgattgta ttgatgacgc tcgctccttc
    39001 aactccattg tttactaata aagtcagacc ccaaataaaa atacttgcaa ataaaataga
    39061 aggaacattt tgtccacctt tgaatatcgg gaaaaattct cccatggcgc tcatcagcat
    39121 cgttgcaaaa gcaacatttc ccagccacgc agacaaccag taaccccaac cactgatgaa
    39181 gccacctaat ttaccaaatg aagcttccgc gtaaccgaaa ataccaccat ctagatctgg
    39241 acgcttttct cctaaatgat tcagcgataa taccaaagct aaaataccaa tcccaacgat
    39301 tatccatgaa aggatcgctg gccctggagc tgcagaacta gccagatcac ttgtgatccc
    39361 aaacacaccg cttccgattg acgaactgac aaccagagca accagtcccc atttgctaat
    39421 acccgtcgat ttcttttcca tttccatcac cctcacttat aaaattgaac aaatcacttg
    39481 caaaagattc ccttctattt tgcaactgct cttgcttaac aaaaagcaac tttattcata
    39541 ttattggata ttatgaactt tttacacaag tatttcaagt ataaaatgaa taaaaattaa
    39601 ttttattgca aaaaaacagc tatgctatcc aaaagaaaag gtgtttcttt tgttagccat
    39661 agctgttttg attcatttaa tatcgcttaa acgtattgta aaacttttgt ttcttgatat
    39721 gcgtggtcga tcaaaccacc acccagacat tccattccat cataaaatac aactgcttgt
    39781 ccaggagtga ttgcacgtac aggttcgtcg aatacgactt ctgcacgatt atcatccaat
    39841 aagcgaactg tcacaggaac gtcttgctga cgataacgga atttcgctgt acacttgaat
    39901 tcttttggca ttggttcatt cgtcgtaaag tggatttcac ttgcatccaa gcttgtggca
    39961 tacaatgcag gatgatggaa cccttgcccc acatataatg tgtttgtcgc tagatctttt
    40021 cctacaacaa accagggttc ttgcgaatct cctccgccac cgatccctag tccttgacgt
    40081 tgaccaatcg tataatacat caagccagca tgttgtcctt tgacttcacc atcaagagtg
    40141 accatatttc ctttttttgc tggcagatag ttgctcaaaa attgtttgaa gttcttttca
    40201 ccaataaagc agattcccgt tgaatctttt ttcttagcag tcgcaaggcc tgcgcgttca
    40261 gcaattgccc gtacttctga tttttccatg ccgcctagag ggaacatcgt ttttgaaagc
    40321 tgttcttgtg ataattggct aaggaagtaa gtttgatctt tattattgtc tactccacga
    40381 agcatgtgga ccacgccgtt ttcatcgcga gtgacttgcg cataatgtcc agtagctaca
    40441 taatcggcgc caagatccat tgcataatcc aagaatgctt tgaatttgat ttctttgttg
    40501 cacatcacgt ctgggttagg tgtacgtcct gcgcggtatt cggctaggaa atattcaaat
    40561 acacgatccc aatattcttt ttcaaaattc acagagtaat aaggaatccc gatttgagca
    40621 gctactttcg ctacatcctt ataatcttcc gtggccgtgc atacaccgtt ttcgtccgtg
    40681 tcatcccaat ttttcatgaa tattccgatc acgtcgtatc cttgttcctt taacaaaagt
    40741 gctgttacag atgagtcgac accgccgctc atccctacga caacgcgtgt tttgctgttg
    40801 tctgtcatga tatcaccatc atttcaatta gattctgaaa catctacgag ttgaaggtcg
    40861 caacttttca gtattttaca ttatacgtca tatttctgaa aatttcatcc cttaattatc
    40921 aagtcgtaaa aatactgttc tactggacct attcagtaca caaacatgat agtcaaccaa
    40981 aaaatcactc atcttttgag cattcgatat aaaaaattgg catactcata aaaagggcca
    41041 gcctttcggc aataaggaat aattttataa attgtgaaaa actatttttc aaaattgctt
    41101 cttatcgctt aaagctgcac gtctcattta caaccttttc tttttagatg ttatgagttg
    41161 ctcagataag tgattttgtt tactctaaga aaaaagggag gcaaaagata catgaattac
    41221 tttatcagtg atatgcactt tttccatgaa cgcttattag gaaaaaacga ctttgctccg
    41281 cggctctttg caaatgtgga tgaaatgaac caacagttga tcactagctg gaatgcagta
    41341 ataaaagaaa ccgaccatgt ttatcatcta ggcgaccttg ctatgcatcc gcaatatgaa
    41401 aaagcaaatg cagaaatttt ggaactggtc actcaactgc aaggaacaat ccacttgatc
    41461 aaagggaacc atgacagtcg cgcttttttc cgttatttag aaaaaaatga tcctaggctt
    41521 actgacggaa gtcccaaatt ttatttttat gatgtaggtg ccatcgttaa attcgaccat
    41581 caacaatatt acttgactca ttatccgatg ttgctcgggc ctaacgacac tatccgcaat
    41641 cttcatggac atatccatca ttacagcgta cctatcgaga acgatgtcaa tgtaggcgta
    41701 gacgcaccgg aaagagaact cttaaaggaa aaactaccat ttggcactcc gttatctgag
    41761 caacaaatca aagaaatcta tgaagcaaag aggaagtacg ataggtcgtg aaaaaactgc
    41821 cctatatcaa gtattaaaca aataaagata attactcata tttttcattt gagtaggctt
    41881 attgccacag gaacattagg atcagaaatg gagttgtaac aagacttctg tcacaactcc
    41941 attccatact attcgtcttt ttgctcatgg tgttctttta ccagtttttt ctgtaattct
    42001 ttatcataat ttcgggcagc tgacgttaaa aataaaacga ttccgcataa taaaatcccg
    42061 attccggaaa acaagaacat tttttctacc cctaaacgat cagctaatgg tcctgcaaat
    42121 aataatccaa ttggacctgt caggttcatc aaagagttga agttccctaa gacacgacct
    42181 aaaacattcg attcataact ctgctggatc attgccatca gcagtgtatt gaaatatggc
    42241 gttgcacaac ctgctcctgc attcaagata agaaagtaca agaacccttg actatcgttt
    42301 ggaaccaatc cgcttgcacc gatcgtcagg cccataatga tgtaggctat aataataggt
    42361 ttcattcggt ctttccatgt tcctataaaa ctgatcagtg caccgcctaa gagcattccg
    42421 actgcgtaga ctacttccac tgctcccgcc tgtccaacgg aaccaccaaa gtaatctagc
    42481 gtcatcaatg gatacagact gattgctggc atgaacaaca gcatcacaaa tgcaccattg
    42541 atcgttatgt accataaccc tttattatcc atcaattgct gcagcccaaa ttttgcatct
    42601 ttaaaaatag taagcggaac ttccaatatt tcaggagaaa cttttgggat cttcacaaaa
    42661 atcaataagc cgacaccaaa aactgccccc aatacatcta ataaaataag gtaattgaca
    42721 ggaacgactg aaaacaaagc cgcacctaga gctggcgcaa tgatgaaatt cgctgattgg
    42781 accattccta actgtccatt cgtccttgtt atgtgagaag atggaacgat tgtcggcata
    42841 atcgactgga tcgttggcat ttggaatgtt tgtgcgaccg aacgcataaa caaagacaca
    42901 aaaactagcc aaatcgggaa ggattctgaa atcgttccga ctactgctaa aataagcgcg
    42961 aaaatcgcta caataatatc cgtgacaatc aaaagagctt ttttatccca gcgatccact
    43021 agcggtccta caaatgggct tagtaaaacc atgggaatca ttcctagtaa agtagcaaaa
    43081 ctcaaaattg tcgccgaacc ggtttctctt gtcaaatacc agatgatcgc atattggacg
    43141 accatactag tgattcctga caaaaactgt cccgataaaa agaacaaata atttctcttc
    43201 caattcgtta aaacgaccga ctgttcattt tccataaaca tccctctttt ctttttcatt
    43261 ttgtatagcg tttgcacaac aatcattata cgggattctt ttttcaaaaa ataccttttt
    43321 ccctaaataa caatcgtttc aatttaaatt tctttactta gaaacaataa atcagcaaaa
    43381 tattaaaaat aacaacatgt gttaaattta aaaataaaaa agacaactaa tttgcgtcgt
    43441 ccttacaaag ttagtcatct aacaataggc cgtttcaaaa ctagttgtct ttttatatcc
    43501 acagatcatt cgactttttc gaataaccca cggctaatca tcccatccat cagaaaataa
    43561 aatttatctt ggctcatttt ctgttttgga tctttgaaaa tattgactgc tttcaatcca
    43621 tttgctgtag tgatcgacat tttcaatgtt tgaatatctt ttgatacagt gatttttaat
    43681 ttgaatcctt ctttgctttg cggcgtggca tctaatggtc ggatcaattg ataattgcct
    43741 ccgtttgttt ctgtaaatcc attatcgcgc aatgtatatc tttttgcatc aggatgcaaa
    43801 cgatagctaa ttggtgcgcc cagtaccgtt gctgttgttt caaatgccat gattttcacc
    43861 tcgataaaat tagtttctct ttattataaa agtttatcct gatttttact aggtttttca
    43921 gttaagttcc ctaaaaaatg aaggcgttct ctcagacatc tgttcatata agaatgactt
    43981 cactagttat tttttgacag cacgaactaa ttgatcaagg aaatactgga tgtcgtcttc
    44041 ggtatttccc aaaccaaagc tgatacggat ggattctttt agtatcggtg tatcttctcc
    44101 atacattgct tccaatacgt gtgaaggctc gatatttcct gctgtacaag ctgaaccagt
    44161 agaaatagcg accccattaa gatcaagctt catcagtagc aaatcatttg cttcgccaat
    44221 taagcgaaga ttcaacacat gagggagttt attcgttaaa tcaccattta cttgataagg
    44281 gatttcgttt gcttctaatg tatcaagaat caattgctgg aatcctttat atttgttgtt
    44341 acgtgcttca cgttcttcag gtgacagcag ttcaactgct tttgccatac cccagatccc
    44401 tgctaaattt tctgtacctg cacgccgctt ttcttcctgc tccccgccta aaagcaatgc
    44461 agggatattt actccgtctc gtttatataa gaaaccgacg ccttttggcc cattgatttt
    44521 atgagcagag atacttaata aatcgattcc taattcttct ggatggatcg tttggtttcc
    44581 gtacgcttgt accgcatctg tatggaaata agctggatga tccgctaaaa gttcaccgat
    44641 ttctttgatt ggcatcaaat ttccgatttc attgttcccg tacatcacag agaccaagat
    44701 cgtatcttct cgtaacgctt ttttgacctc ttcgatcgac agattccctt tttcatctac
    44761 aggcagatat gtaacgtcaa acccctttgt ctctaaatat ttcatggtat taagaacagc
    44821 aggatgttcg atagctgtag tgattagatg tctgccttct ttttgacgcg agaacgctgt
    44881 ttctaagata gctgtattat ccccttctgt tcccccactg ttaaagatga tctcgtgagg
    44941 ttttgcttgc agactgtctg caatgatttg tcttgctgtt tccaattttt catgcgcgtg
    45001 gcgaccaaat ccatgtatgc ttgaaggatt tccaaacgtg tcttgcatga ttgccgtcat
    45061 cgcttcaatt acttgtggat gcatgggggt agttgccgca tgatccagat aaatagtttt
    45121 cacttcattc atcccttcca aattattgct aggcacattc tagcataaaa tagcgaacaa
    45181 accaaagaag agctcatttt tgtctagagg acgtttcatg ggcatagaaa aaaggagctg
    45241 tgacaaaagt cttgtcgcag ctccttgaag ctgctcactt ctgtcacaac ttctt
//
LOCUS       DAFCNJ010000018        43367 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000018 DAFCNJ010000000
VERSION     DAFCNJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.018"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(8..2389)
                     /locus_tag="LNE35_001229"
     CDS             complement(8..2389)
                     /locus_tag="LNE35_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK6910400.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="LNE35_001230"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="LNE35_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBK6910401.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="LNE35_001231"
     CDS             3090..3629
                     /locus_tag="LNE35_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBK6910402.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="LNE35_001232"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="LNE35_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBK6910403.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="LNE35_001233"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LNE35_001233"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="LNE35_001234"
     CDS             4736..5902
                     /locus_tag="LNE35_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBK6910404.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="LNE35_001235"
     CDS             5948..7444
                     /locus_tag="LNE35_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBK6910405.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="LNE35_001236"
     CDS             7525..8208
                     /locus_tag="LNE35_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6910406.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="LNE35_001237"
     CDS             8853..10043
                     /locus_tag="LNE35_001237"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBK6910407.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="LNE35_001238"
     CDS             10145..11098
                     /locus_tag="LNE35_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBK6910408.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="LNE35_001239"
     CDS             11167..12516
                     /locus_tag="LNE35_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6910409.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="LNE35_001240"
     CDS             complement(12699..13022)
                     /locus_tag="LNE35_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910410.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="LNE35_001241"
     CDS             13151..14314
                     /locus_tag="LNE35_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK6910411.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="LNE35_001242"
     CDS             14384..15733
                     /locus_tag="LNE35_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6910412.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="LNE35_001243"
     CDS             complement(15778..16209)
                     /locus_tag="LNE35_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6910413.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="LNE35_001244"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="LNE35_001244"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBK6910414.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="LNE35_001245"
     CDS             19439..20035
                     /locus_tag="LNE35_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6910415.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..21305
                     /locus_tag="LNE35_001246"
     CDS             20157..21305
                     /locus_tag="LNE35_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6910416.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21343..21756)
                     /locus_tag="LNE35_001247"
     CDS             complement(21343..21756)
                     /locus_tag="LNE35_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBK6910417.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21835..22266
                     /locus_tag="LNE35_001248"
     CDS             21835..22266
                     /locus_tag="LNE35_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBK6910418.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22365..22598)
                     /locus_tag="LNE35_001249"
     CDS             complement(22365..22598)
                     /locus_tag="LNE35_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBK6910419.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22781..24451
                     /locus_tag="LNE35_001250"
     CDS             22781..24451
                     /locus_tag="LNE35_001250"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBK6910420.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24598..24984
                     /locus_tag="LNE35_001251"
     CDS             24598..24984
                     /locus_tag="LNE35_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910421.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            24971..25669
                     /locus_tag="LNE35_001252"
     CDS             24971..25669
                     /locus_tag="LNE35_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6910422.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25674..26138
                     /locus_tag="LNE35_001253"
     CDS             25674..26138
                     /locus_tag="LNE35_001253"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBK6910423.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26135..27046
                     /locus_tag="LNE35_001254"
     CDS             26135..27046
                     /locus_tag="LNE35_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK6910424.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27281..27820
                     /gene="pyrR"
                     /locus_tag="LNE35_001255"
     CDS             27281..27820
                     /gene="pyrR"
                     /locus_tag="LNE35_001255"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBK6910425.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27834..29117
                     /locus_tag="LNE35_001256"
     CDS             27834..29117
                     /locus_tag="LNE35_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBK6910426.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29130..30056
                     /locus_tag="LNE35_001257"
     CDS             29130..30056
                     /locus_tag="LNE35_001257"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBK6910427.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30075..31355
                     /locus_tag="LNE35_001258"
     CDS             30075..31355
                     /locus_tag="LNE35_001258"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBK6910428.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31360..32436
                     /gene="carA"
                     /locus_tag="LNE35_001259"
     CDS             31360..32436
                     /gene="carA"
                     /locus_tag="LNE35_001259"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBK6910429.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32441..35626
                     /gene="carB"
                     /locus_tag="LNE35_001260"
     CDS             32441..35626
                     /gene="carB"
                     /locus_tag="LNE35_001260"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBK6910430.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35680..36456
                     /locus_tag="LNE35_001261"
     CDS             35680..36456
                     /locus_tag="LNE35_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBK6910431.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36453..37385
                     /locus_tag="LNE35_001262"
     CDS             36453..37385
                     /locus_tag="LNE35_001262"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBK6910432.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37382..38098
                     /gene="pyrF"
                     /locus_tag="LNE35_001263"
     CDS             37382..38098
                     /gene="pyrF"
                     /locus_tag="LNE35_001263"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBK6910433.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38105..38794
                     /locus_tag="LNE35_001264"
     CDS             38105..38794
                     /locus_tag="LNE35_001264"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBK6910434.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            38767..39036
                     /locus_tag="LNE35_001265"
     CDS             38767..39036
                     /locus_tag="LNE35_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910435.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39074..39715
                     /locus_tag="LNE35_001266"
     CDS             39074..39715
                     /locus_tag="LNE35_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6910436.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39758..40450
                     /locus_tag="LNE35_001267"
     CDS             39758..40450
                     /locus_tag="LNE35_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBK6910437.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40447..41349
                     /locus_tag="LNE35_001268"
     CDS             40447..41349
                     /locus_tag="LNE35_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6910438.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="LNE35_001269"
     CDS             complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="LNE35_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBK6910439.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13874 a         7663 c         9440 g        12390 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8281 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8521 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9481 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21301 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21361 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21421 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21481 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21541 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21601 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21661 tagctgttct tgtttacctt gatagacaat caagattcca cctccactag gaccagggtt
    21721 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    21781 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    21841 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    21901 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    21961 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg ttcttttatt
    22021 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22081 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22141 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22201 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22261 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22321 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22381 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22441 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22501 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22561 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22621 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22681 gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct taacaaagaa
    22741 gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg atctgcaaat
    22801 ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag atcttaagga
    22861 agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg gaatcaatcg
    22921 atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc caacaccagc
    22981 tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata aaatcaataa
    23041 aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa tcaaaggtgg
    23101 tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact tgcattttac
    23161 tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt tagataatca
    23221 tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga aacgggtagt
    23281 cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc caatccaagg
    23341 tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg ctatcttgtg
    23401 tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg tcggctatac
    23461 atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc tggcattact
    23521 gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac cagcttttgt
    23581 ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag ctaccaagac
    23641 agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag atcttggcgg
    23701 agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg ctgtggtgat
    23761 tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg aacttaaaaa
    23821 tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata tacgtaatat
    23881 ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag atacagacaa
    23941 tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca agagagcgag
    24001 tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag tacgattgat
    24061 tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga tacaagaaaa
    24121 gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca gcaaaaaagc
    24181 gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga tttgtatggc
    24241 aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg aagatttcac
    24301 catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag ccttgactgg
    24361 agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata tggacgtgac
    24421 aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca tcgaaggagc
    24481 tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt ttacactagg
    24541 atcattcttt ttctttattg tattttgagc tatactaaag cataatagga gggtatgatg
    24601 aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat tcagctaaga
    24661 atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct tgatctggaa
    24721 gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta tacagtgaaa
    24781 gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat ctatccgttt
    24841 ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt cagttttttt
    24901 gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata tttgatcaag
    24961 gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa tcgaaaaatg
    25021 gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg tcagacgcct
    25081 agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac agcttgctca
    25141 gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg cagagccaaa
    25201 tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc ctgaaaccgt
    25261 gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt tacttgaact
    25321 gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata aaggaaaatt
    25381 tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa gccaaaaagg
    25441 tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg gaaagtacac
    25501 aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga tgtttcgtga
    25561 gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg gtaatctgct
    25621 cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat tttttgttaa
    25681 ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc aaatggtgga
    25741 tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt ttgtcactga
    25801 atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg tggttcttta
    25861 ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa aatcgtgaaa
    25921 aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg attggcaatt
    25981 ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat tttatgagct
    26041 ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt atgttaattt
    26101 atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa cgacgattca
    26161 ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt acagtcgttc
    26221 gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc cagtcaaagc
    26281 aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac cagaaacatt
    26341 agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag atgtagcggt
    26401 gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc atggcacaat
    26461 ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg ttattcgtcc
    26521 aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg ccaaaaatga
    26581 ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc gcaaatatgt
    26641 tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc caatcggccg
    26701 ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg ctgtcacaca
    26761 ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac tagaaaccgg
    26821 aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag ctggagatcc
    26881 agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg cgcaaatgtt
    26941 aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc ttccagaagt
    27001 ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta gataacggta
    27061 tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta gtcccgtgag
    27121 gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg ctcattgaca
    27181 tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct gtcctcttag
    27241 ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa aagaagtagt
    27301 agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta tcgagagaaa
    27361 ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct acattgcatc
    27421 aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag gagaactgga
    27481 tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac ttcattcttc
    27541 tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg ttttgtatac
    27601 aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac caagaaaaat
    27661 ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg cagattatgt
    27721 tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc aagagcttga
    27781 tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga aacatgtcgg
    27841 agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc ccaaccccag
    27901 gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca accgtgttag
    27961 tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga ctaggtacat
    28021 tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc agcttcgctt
    28081 tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt gcacaaggag
    28141 cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag atcggttcag
    28201 catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg gttatcgggt
    28261 tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg tatgatttca
    28321 agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac atgtggctaa
    28381 gaggttttct cggattgatt ccgattttac taggtattat tagcggatac atcgtagcct
    28441 tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt gctgttccta
    28501 actttgagat tccctttgta caatatgaac caaagctata tcttggagca atcacaacaa
    28561 tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg gtattgaata
    28621 aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg acaggtgatg
    28681 gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt tacggtgaaa
    28741 acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt ggagctgcag
    28801 tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta agtatccctg
    28861 gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca agtggaatga
    28921 agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg attgcttcag
    28981 ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt acattgtcgg
    29041 caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca gaaacttcac
    29101 gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt atcagcttaa
    29161 agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg atccgcagag
    29221 ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac tttgctacaa
    29281 acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg gagaaaaaac
    29341 tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa ggggaaacac
    29401 tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt atccgtcatg
    29461 gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt tcgattatca
    29521 atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg atgaccattt
    29581 atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat atcactcact
    29641 ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa gtattcttct
    29701 caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat atgccattgg
    29761 atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac gaacgccatg
    29821 atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta acagttgagc
    29881 gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt aaccgtgatg
    29941 tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag caaatgagca
    30001 atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa gcgtaattat
    30061 aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca cgaaaaaata
    30121 tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc ggtacaggat
    30181 tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc acacctggtt
    30241 tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag acgatcgaag
    30301 ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg ccgaatttaa
    30361 atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt agaaaagatg
    30421 cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt gagaaattag
    30481 ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac ggtgtaggtg
    30541 tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac aataaagccc
    30601 tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac gctggcaaga
    30661 aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct caaattgcta
    30721 gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat gtttctacta
    30781 aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta acagcagaag
    30841 tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc ggcttttgga
    30901 aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa ggcttgcttg
    30961 acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa gaaaaaagcc
    31021 aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt caactgatct
    31081 atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat tggttggcag
    31141 ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt gcccccgctg
    31201 atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac tttttatcaa
    31261 aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa atgacttttg
    31321 tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt gcttatatta
    31381 gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt cttcggagaa
    31441 atcgttttta caacaagtat gactggttat caagaaacta tcactgatca aagttttaac
    31501 ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa tagagatgat
    31561 tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc acgtgtagct
    31621 tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg aattcctgga
    31681 atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg aacgatgaaa
    31741 gctagtatag ttgatgctgg agattccttc gaacatgcat ttgatcagct gaaagcaacc
    31801 gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc aagtccagga
    31861 acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt acgagaattg
    31921 agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga gacgatttta
    31981 gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa agatgtgcca
    32041 gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg tatttgtctc
    32101 ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa attcggacat
    32161 cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt tacttctcaa
    32221 aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat cgtaactcat
    32281 gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc cgctttcagt
    32341 gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt atttgatgaa
    32401 tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac gtacagatat
    32461 caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg cagaatttga
    32521 ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag tggttttagt
    32581 aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag tatatatcga
    32641 accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag atgctctcct
    32701 tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag catcaggtat
    32761 tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg atcaagcaga
    32821 agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc ccgagtctga
    32881 aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct atcctgtcat
    32941 cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa atgaagaaga
    33001 attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat gtttgattga
    33061 acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt cggctgataa
    33121 tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata caggtgattc
    33181 tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc ttcgtgatgc
    33241 ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc aactcgctct
    33301 tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt ctcgttcatc
    33361 tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta aaatagcgct
    33421 tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg cagaatttga
    33481 acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata agtttgaaaa
    33541 cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg ctatcggtcg
    33601 gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta cacaccatat
    33661 cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag tggttcgagc
    33721 acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca ccatagaaga
    33781 attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc atatcgtaga
    33841 aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa cagcgaaaca
    33901 aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag aagccgttcg
    33961 ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca cttgtgcagc
    34021 ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa atgaaagttc
    34081 acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta tcggacaagg
    34141 ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg caggatacga
    34201 agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta tttcggataa
    34261 attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc tagaacagcc
    34321 gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg aacctttgac
    34381 tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg cggaaaatcg
    34441 tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag gggatactgc
    34501 tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag ttcttgttcg
    34561 tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc gagatttaga
    34621 agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag tggaccgtta
    34681 cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag tactcatccc
    34741 agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa tggctgtgta
    34801 tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca cgatccgctt
    34861 agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc atgaaaatga
    34921 ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct taagtaaggt
    34981 gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga aactaaagac
    35041 acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta aagcacctgt
    35101 cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa tgaaatcgac
    35161 tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg cattcgaagc
    35221 ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg acgaaacaaa
    35281 agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc tgataggaac
    35341 gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag tagcgaaaat
    35401 ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta aaacacaagt
    35461 ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt tcatcatccg
    35521 aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag cagatgcgat
    35581 cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc gagcacatgt
    35641 taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga aatgatggaa
    35701 atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa aggtgatcta
    35761 gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg tgcagacttg
    35821 ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac ttgccgtatc
    35881 atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc cggagaccat
    35941 ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca tacaggagac
    36001 gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt atcaaaacag
    36061 ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca tgaagtcgtt
    36121 tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga cgatggaaca
    36181 tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga acctacagct
    36241 gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt ttcttcgcat
    36301 caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc ttgctatgct
    36361 tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt ttgtgacgaa
    36421 ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta gcggtcaaac
    36481 ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc ggttttggca
    36541 aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc aaggcaacga
    36601 ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca tctggcatgt
    36661 tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa cttcttccag
    36721 aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct tgtgaagaag
    36781 actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca atcgagctga
    36841 acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat tctgatgttg
    36901 cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat gtcaaattgt
    36961 ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga ggggcagatg
    37021 ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca agaaaaccga
    37081 ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc gctattcgtt
    37141 tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc ggcgtgcaga
    37201 cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct atagggaccg
    37261 cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct gtgcgaatga
    37321 gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag gaacgtgaac
    37381 gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta ggcaagaggt
    37441 agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa tcggaatgga
    37501 attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg gtcataatgt
    37561 atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa tgcggggatt
    37621 ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc gtatgatgga
    37681 ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc cgctacttct
    37741 agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc aactaatcga
    37801 agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag caggattaga
    37861 tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag gagacgaatt
    37921 tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc agacaagagt
    37981 agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag gtcgaccaat
    38041 cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat ggagctaacc
    38101 taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag cagtattttt
    38161 aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt actgtgataa
    38221 ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat tagcaagcaa
    38281 aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag caggaatccc
    38341 tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc gaagtaaggc
    38401 gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc agaagatggt
    38461 tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg aagcagcaaa
    38521 aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt tgccaaaagg
    38581 aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt attctgtatt
    38641 gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc tgctaaaaga
    38701 atggaaaaaa gatcctgaaa attggcagat tgaagcgcgc ttgatgaaga tagagaaatc
    38761 cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact gattgcgaga
    38821 agcatctttt catcaggtat gaaaagaaac ttctatcgtg atcagttttt gattttttta
    38881 aaattcattt ttctaagact ggttagcaga aagaagagaa taatctcttc tcttttttct
    38941 attatatata gattttctct attttttggt aagggagctg tttcaaaaat ggtaaagagc
    39001 ggaaatgttg cgaatatcag ttatttaatg atatgatatt cggaaaaaga aatctcaaag
    39061 ggaggtaact tttatggggt tgaatgaatt tattttagga ttgaacaatt tagagacgat
    39121 cacaagggca cctggatttt ttaaatatac agagcataca gttgcagcac attcgtatcg
    39181 tgtagcatct attgcgcaag ttctaggaga cattgaagaa gcgaatggac aaccgatcga
    39241 ttggaaactg ctctatgaaa aatcattgaa tcatgactac acagagcgtt ttattggtga
    39301 tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga gggatgctgc aaacagttga
    39361 agaaaagatg acggacgaat tcatcaacca agagattcca gctgagttcc aagaagttta
    39421 tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa ggagaactat tagccatttc
    39481 agataaggtt gaccttcttt atgaatcttt tgatgaaatc atcaaaaata acccggaaaa
    39541 agtttataag gagatgttca ttgaggcagt gactacgatt aaaagctttg aacatcggcc
    39601 ttctgttcag tatttcttta aagagatatt tccggaacta ttgaaccagg aattttatgg
    39661 aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag aaagaagaaa aataaaaatg
    39721 atatgagatg cagggggaag tatagttgaa aaagaatatg gatgtcgaat ggggctattc
    39781 tggagacaaa ggtccggaaa attgggcgag tttatgtgat tggtttgcga gaggtgcgga
    39841 atttccattg cagtcgccta ttcatttgac caagggggaa gaaaccaaaa tagaaggaga
    39901 cacgctttcc tttcattatc aaaagcaacg gtttactgac aaggaattca aaaacacgat
    39961 tcatttagta ccttttgacc aactcagtta tgtagagttc aagaaagaac gctattattt
    40021 gacagatatt catttccact tgccttctga acatatcatc aacggaaaac aggaagatat
    40081 tgaatttcat tttgttcata tgaacagcaa aaaagagaat ctagttgtgg gtgtcatgta
    40141 ccagctaatg gtacaggaag gttggctgta taatcaagaa aatggggaaa gttggaactt
    40201 agaaaaacat gagcattggg taaatccgga tgtgttcttt ccggagcaaa aaagccattt
    40261 ccattatatt ggctcattga caactccgcc taccagcggg ccaatccaat ggtttgtaat
    40321 ggatcatgta ggaaaactga accaagagtt tcttctcccc ttcgatggtc agtatgctcg
    40381 accgaacaac cgaccaatcc agcccttgaa tggaagagaa atttattatt tcgaggagtt
    40441 tgagcaatga atattcagca gatgaaatac gtggtcgctg tagcaaacaa tggtagtttc
    40501 agagaagcgg caaagaaaat gtttgtctcc cagccaagtc tttcagctgg tatcaaagaa
    40561 ttagaaaccg agttagggat cacgctgttt actcgaacga atcgaggggc atatttaaca
    40621 gaagaagggc aagacttctt aaatcgtgcc gagaaaatat tggtccagct tgatacatta
    40681 gaaactcact atctaacgag tgaaaagaga gacaattttt cgatcgctgc ccagcattat
    40741 gatttcttag gtcctttgac cgcacagcta ctcgaggaat tcaaagacga gatcaaacaa
    40801 tttcggatca tggagacgac aacagcgaga gtgatcgaag aagtcaaaga gcagcacagt
    40861 gagatcggca ttatttatat gaatgagcat aatcaagctg gtatcagaag atatttacaa
    40921 caaggagagc taagtggccg cagcattgga aaattcaaaa cgcatatttt tttaaggcaa
    40981 ggacatccat tagctgaaca cgcagagatc accaaagaag aattgttatc ttatcctcaa
    41041 gttcgtttta cacaggatgg aaataatttc ccgtattttt atgaagacct tatcgagatc
    41101 cctgatcaag aaacagtgat ttatacgagt gatcgaggta ctttgatgaa tatcgttctt
    41161 gaaactgatg cttacgcttc aggatctggt attgtgattg gagggattcg cgagcacttg
    41221 cggttaatcc ctttggcaga aggcgaacta aatgaatttt atatcattta ttcggcgaaa
    41281 cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc ttactcggtt actggaccag
    41341 cagagctaag aaaagcagat gagaacgctg tttattgaac accgttgatc aaaaaagagg
    41401 ggttgtgaca gaattttgtc acaactcctc tttttgtagc aaacaattta taactaattg
    41461 ctgttgtggg aaagtacgca aatatgattc tagaaacact cattgattac ctgttttgtt
    41521 tcatagctgt aattttttct tcttctggtg tcacgataat cgtcttttgg ttttcataaa
    41581 taacataacc aggcttggcg ccatttggtt ttcggatatg ctttacctgt acaagatcaa
    41641 ccggcacttg ggcagagtat cggtattttg aaaaataggc agctaactcg gcagcttctg
    41701 taattgtttc atctgagggc ttgtcacttt taatgatgac gtgcgaaccg ggtatatttt
    41761 ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat tgtcagctgg tcattttgaa
    41821 gattgttttt accaacaaga atgagcgttc cgtcagaaga aaagtattga tcaggctgag
    41881 atggtttctt acgtttttgt ttttttaagt tcttcttttt caggtatcct tctgaagtca
    41941 attcttcttt aattgcttcg atatccattg ggccggcaat ttccagttga gataagacag
    42001 attcaagata ttggatttca tctttggctt cttggatttg ttcgccaatc agcttaacag
    42061 cgttcttcag cttttggtat cgatggaaat atttctgtgc attttgattt ggtgtcaatg
    42121 ccggatctag tgctattttg attggccgat cttcttcata ataattaggt aaaacaactt
    42181 cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag tagttcgcca tttctacggt
    42241 aattctcagc gttttcggat tctttgagtg tttgttcacg ttttttcaat ttatttttat
    42301 ttcgttttag ttcattttcg atttttcgaa tcagttctcc gccttgttgt tttgcccgat
    42361 ctttctctgc tttttcgtga taatagctat ctaataattg actgagtgtt ggataagctg
    42421 ttacagcaga tgcctgttcg gacaatacct gataagcaat cggtgtgaaa aaatctttat
    42481 tttccgtttc gtaaaaagta ggaataggct gatggctgat ggctgagaaa aattcgtccc
    42541 atacaaccat cttttcatta ggacgttcca gtaatctttt ggtcagctct tgagcgatat
    42601 cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg atctagttca gtttgggaaa
    42661 gccgctgaaa gattttttct ttctcggagc taaatgggtt caagacctct tgttttggcg
    42721 gagcaacata ttctacacca ggaagcaaag aacgataagt gttttgagag cttccaatgt
    42781 gtttgatcgc atccagtatt tttccagttt ctcgattaac taagacgatc gtactatgac
    42841 gtcccattaa ttcaacgatc aatatgatat tttgtagatc acccaactcg tttcgtttag
    42901 caaagtggaa atggatcact cgatcatttt cgatttgttc gatgtcttct aaaatagcgc
    42961 catccaaata ttttcgaagc atcatcacga agttaggcgg attatcagga ttctgataat
    43021 cgatttgggt aatctgtaca cgagcataac ttgggtgcgc agataataat aatcgatggt
    43081 ttttgccgcg tgaacgaatc accagcacga tttcattttc ataaggctga tggattttcg
    43141 agatccggcc agatagtaac gtttcacgca attcgttgac catcgcgtgg gtaaatacac
    43201 catcaaagga catggttcat tcctcatttc ttttcataag tatattcatt ataacgaaaa
    43261 aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag tctagaaaaa tcttggacta
    43321 attacataaa aaacagtgtt tctatcgttg aaatccaaat ttttagg
//
LOCUS       DAFCNJ010000019        40953 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000019 DAFCNJ010000000
VERSION     DAFCNJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.019"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(28..330)
                     /locus_tag="LNE35_001270"
     CDS             complement(28..330)
                     /locus_tag="LNE35_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910440.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(579..779)
                     /locus_tag="LNE35_001271"
     CDS             complement(579..779)
                     /locus_tag="LNE35_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6910441.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(1084..2316)
                     /locus_tag="LNE35_001272"
     CDS             complement(1084..2316)
                     /locus_tag="LNE35_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK6910442.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2570..3139
                     /locus_tag="LNE35_001273"
     CDS             2570..3139
                     /locus_tag="LNE35_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6910443.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3317..3757)
                     /locus_tag="LNE35_001274"
     CDS             complement(3317..3757)
                     /locus_tag="LNE35_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBK6910444.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3915..4679
                     /gene="map"
                     /locus_tag="LNE35_001275"
     CDS             3915..4679
                     /gene="map"
                     /locus_tag="LNE35_001275"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBK6910445.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4711..5634
                     /locus_tag="LNE35_001276"
     CDS             4711..5634
                     /locus_tag="LNE35_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBK6910446.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5710..6849
                     /locus_tag="LNE35_001277"
     CDS             5710..6849
                     /locus_tag="LNE35_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBK6910447.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6842..7642
                     /locus_tag="LNE35_001278"
     CDS             6842..7642
                     /locus_tag="LNE35_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6910448.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7642..8469
                     /locus_tag="LNE35_001279"
     CDS             7642..8469
                     /locus_tag="LNE35_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6910449.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8447..9181
                     /locus_tag="LNE35_001280"
     CDS             8447..9181
                     /locus_tag="LNE35_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6910450.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9281..10147
                     /gene="rfbA"
                     /locus_tag="LNE35_001281"
     CDS             9281..10147
                     /gene="rfbA"
                     /locus_tag="LNE35_001281"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBK6910451.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10161..10733
                     /gene="rfbC"
                     /locus_tag="LNE35_001282"
     CDS             10161..10733
                     /gene="rfbC"
                     /locus_tag="LNE35_001282"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBK6910452.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10755..11783
                     /gene="rfbB"
                     /locus_tag="LNE35_001283"
     CDS             10755..11783
                     /gene="rfbB"
                     /locus_tag="LNE35_001283"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBK6910453.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11881..12732
                     /gene="rfbD"
                     /locus_tag="LNE35_001284"
     CDS             11881..12732
                     /gene="rfbD"
                     /locus_tag="LNE35_001284"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBK6910454.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12767..14800
                     /locus_tag="LNE35_001285"
     CDS             12767..14800
                     /locus_tag="LNE35_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910455.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14844..16124
                     /locus_tag="LNE35_001286"
     CDS             14844..16124
                     /locus_tag="LNE35_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBK6910456.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16334..17140
                     /locus_tag="LNE35_001287"
     CDS             16334..17140
                     /locus_tag="LNE35_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910457.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17152..18372
                     /locus_tag="LNE35_001288"
     CDS             17152..18372
                     /locus_tag="LNE35_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910458.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSNGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18362..19948
                     /locus_tag="LNE35_001289"
     CDS             18362..19948
                     /locus_tag="LNE35_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6910459.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19987..22125
                     /locus_tag="LNE35_001290"
     CDS             19987..22125
                     /locus_tag="LNE35_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6910460.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(22493..23476)
                     /locus_tag="LNE35_001291"
     CDS             complement(22493..23476)
                     /locus_tag="LNE35_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBK6910461.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23854..25245
                     /locus_tag="LNE35_001292"
     CDS             23854..25245
                     /locus_tag="LNE35_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK6910462.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            25261..26301
                     /locus_tag="LNE35_001293"
     CDS             25261..26301
                     /locus_tag="LNE35_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6910463.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            26320..27405
                     /locus_tag="LNE35_001294"
     CDS             26320..27405
                     /locus_tag="LNE35_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBK6910464.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            27438..28400
                     /locus_tag="LNE35_001295"
     CDS             27438..28400
                     /locus_tag="LNE35_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK6910465.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            28393..29820
                     /locus_tag="LNE35_001296"
     CDS             28393..29820
                     /locus_tag="LNE35_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBK6910466.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            29822..30892
                     /locus_tag="LNE35_001297"
     CDS             29822..30892
                     /locus_tag="LNE35_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910467.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            30879..32300
                     /locus_tag="LNE35_001298"
     CDS             30879..32300
                     /locus_tag="LNE35_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBK6910468.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            32313..33320
                     /locus_tag="LNE35_001299"
     CDS             32313..33320
                     /locus_tag="LNE35_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6910469.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            33332..34336
                     /gene="neuB"
                     /locus_tag="LNE35_001300"
     CDS             33332..34336
                     /gene="neuB"
                     /locus_tag="LNE35_001300"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBK6910470.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            34333..35481
                     /gene="neuC"
                     /locus_tag="LNE35_001301"
     CDS             34333..35481
                     /gene="neuC"
                     /locus_tag="LNE35_001301"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBK6910471.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            35454..36131
                     /locus_tag="LNE35_001302"
     CDS             35454..36131
                     /locus_tag="LNE35_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK6910472.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            36121..37263
                     /locus_tag="LNE35_001303"
     CDS             36121..37263
                     /locus_tag="LNE35_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBK6910473.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            37276..38253
                     /locus_tag="LNE35_001304"
     CDS             37276..38253
                     /locus_tag="LNE35_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6910474.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            38253..39161
                     /locus_tag="LNE35_001305"
     CDS             38253..39161
                     /locus_tag="LNE35_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBK6910475.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            39162..39914
                     /locus_tag="LNE35_001306"
     CDS             39162..39914
                     /locus_tag="LNE35_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBK6910476.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            39919..40866
                     /locus_tag="LNE35_001307"
     CDS             39919..40866
                     /locus_tag="LNE35_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBK6910477.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        13676 a         6253 c         7967 g        13057 t
ORIGIN      
        1 aagcgagttt gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt
       61 atactttata tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat
      121 atcaaacaaa aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata
      181 gttcttatta gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac
      241 ttctgaattc catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa
      301 ccataattgt gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac
      361 gaatgttctt tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt
      421 gccaaattat gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc
      481 tatattaaga tttcctgcaa taacaaacac aacagaagac gtgtaatagt tagtcaggaa
      541 cgtacaaata acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt
      601 gtagtttcta aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga
      661 taaagtaaaa tagatgcatg cgtatgtctt agtccatcaa taaacagtcg aagagaaaat
      721 agggaaaaat tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata
      781 cttcaataga ttcgaattaa acgatttcga ttggcttata ccagtcctat ccaacgaaag
      841 atatatcgtt cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg
      901 aactggagat gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt
      961 ccttttcaaa gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag
     1021 ccaatagctt agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta
     1081 tactcaagcc caatcgccat ttcggaaaat cggtactgta gagccatctt cacggattcc
     1141 gtcgatatcc atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa
     1201 tccagcttct gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata
     1261 agcagaacct agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat
     1321 gatgccagat tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg
     1381 cgcaccttca tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa
     1441 atcaacaact tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata
     1501 actcagtggc tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa
     1561 cacttcttcc gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc
     1621 gccttcccaa agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata
     1681 gtgaagggca gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc
     1741 atgcttcttc catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg
     1801 atcccataat gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca
     1861 ttgtttacca gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg
     1921 taaattcatt agtgctttgc cattcgctga ttgataagac gctactcgat ggctatctac
     1981 accagcaaat gcatcgggat cggcagaaac aacgctgatc ctgcttgctc ctttttcaag
     2041 ccattcatcc gcttgatcga ttttagattg tggtacattc tcaatacgat ccgttgcagc
     2101 atgtaaaaga aattctcttt ggatctggtc gtcagtccat tgaacgataa cctcagcggc
     2161 tcctagttta taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat
     2221 ctgtaaaaca actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc
     2281 atattttttt agaagttcat taaaatcaga taacataata aaaattcctc ctcaccattt
     2341 tttctggtaa ctattagcat gataccattt aattatgatt caaacaatga ggctttaaat
     2401 aaaaaaagga ggttattttt ttaaggttaa gtttcatgat atatgttact acttgctatt
     2461 tcagcttgtg aagaaaatca aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa
     2521 attaatcaat ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa
     2581 aaagacgatt actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg
     2641 atttacacga tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat
     2701 ttatcttgag tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga
     2761 tcatttacaa tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc
     2821 ttttaattat atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga
     2881 agaatatggt ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt
     2941 gaaacgcgat ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg
     3001 gacatggatc atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac
     3061 tgatgaacag attgaaaaga tgatccaaga cagtatagat gctatcttga aattcaaaga
     3121 accaatcgaa atcgattgaa tatactcaaa agttccacaa atcagtaggt aacaagagat
     3181 gtgtcaaaat aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga
     3241 actcttccat gaatgcaatg aataaaagag ccatacagat tgaataaatc tggatggctc
     3301 ttttattgca agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt
     3361 cgatatcttc ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc
     3421 ctgtttttgc aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag
     3481 tatcaccaga tccgcagaca ccgtaaattt ttcccaatag atccaattct tgcaagtctt
     3541 cataaaagtc tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta
     3601 cgcaaatatc tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga
     3661 cttcgatatc cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc
     3721 ctgtcatgct agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt
     3781 atttaacaat tatgaattat accaaaacat cattgaaaag aaaataaaac ctaaagacac
     3841 ctggggaatg tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa
     3901 aaggagacga aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat
     3961 ctggagcttt attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca
     4021 caagttggga tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg
     4081 cgcaaattgg ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt
     4141 gtcatggatt ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt
     4201 gtatcgattt gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta
     4261 cacctgagat cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc
     4321 aagcacaagt agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag
     4381 gcgaaaatct agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg
     4441 aagaaccagc agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca
     4501 tggttatcac gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta
     4561 acggctggac tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg
     4621 ctattactaa agatggtcca agaattttga catcacaagg agaagaaggc acttattgat
     4681 ctatccagtc aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat
     4741 gaaaatctga tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt
     4801 acgacctctg tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct
     4861 tttggtaata ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga
     4921 gaagtgattc ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca
     4981 caaagttcag gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa
     5041 agtatcaatg ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat
     5101 tttttgatcg tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt
     5161 ggggtaacta tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta
     5221 caaatacccg tgaactttat cgatcagttc caaactttga agtggccaat cacattggta
     5281 gctttatttg tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg
     5341 aagacgatca ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg
     5401 tttagtatct atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt
     5461 tttattgtac tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc
     5521 atcaatgcag ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca
     5581 agccgaaaat ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa
     5641 gagttcttat tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta
     5701 ggtgcccata tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta
     5761 tcactgatat taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa
     5821 ccaaatgaaa gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt
     5881 atttcatttg ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt
     5941 gtaccgatct tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat
     6001 gagctgacac ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt
     6061 gtagcagata tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc
     6121 tggcttagtt ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg
     6181 attgacggtt tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga
     6241 atcattggat attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt
     6301 cttgtagcaa gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta
     6361 ggcgatacag gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg
     6421 aagaacgtga cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca
     6481 gacacgtttt ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat
     6541 aaaaaacatc ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg
     6601 acgatttatg cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga
     6661 agaatcggaa cgatcgtatt gattattgcc atgttgtttg ctgcaatttt attatttgaa
     6721 ttgattggtc tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg
     6781 aataaagagt accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata
     6841 gatgaataac aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa
     6901 aatttttcgg acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat
     6961 ccgaattttt gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa
     7021 atatccctac atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa
     7081 tcacaatctg atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt
     7141 ggatgaagag acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat
     7201 gttagcacct aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct
     7261 ggctgttttt gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga
     7321 caaacgactg gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg
     7381 gatgatctca ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt
     7441 tgatgagcgt ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc
     7501 aggttacaag ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc
     7561 gcatcgaaat gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa
     7621 gtgggggtgg cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag
     7681 caactattca gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa
     7741 attcagctta ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa
     7801 gaaaatatcg tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt
     7861 gcgttgtctc aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg
     7921 ctagtcgacg attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct
     7981 gcagttccga tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat
     8041 atcaatcgta cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca
     8101 atcaattcag gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct
     8161 gctttttctt tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa
     8221 actgtggaga tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa
     8281 gtttcaagta atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat
     8341 gatcaaaggc atttatccag tcaccgacga caattattgt taagaacagc taaacagttc
     8401 ttgaccgtca aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg
     8461 aaggtgtgaa ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag
     8521 agagcagtta gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga
     8581 tgatgggtca gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg
     8641 aatccagctt ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca
     8701 gcagacgaaa ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa
     8761 agtgaaaaag atcactgact attttgagca gaatccggat cagctagtag tagtttctga
     8821 tttaatcatt gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa
     8881 ttccaaagaa ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc
     8941 tttccggtct tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga
     9001 tatgtggatt ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct
     9061 gtaccgaaga catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa
     9121 aatttcttgg cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg
     9181 ataaaagatg tttaaatcct taatatttcc atgacaaata taacaattga tgagataata
     9241 acaaagtgaa atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc
     9301 tgggggcagc ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc
     9361 gatttatgac aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa
     9421 agaaattctg attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa
     9481 cggaaatgat ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc
     9541 acaagcgttt atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg
     9601 ggacaatatt tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga
     9661 tacaggcgcg acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga
     9721 atttgatgag aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa
     9781 ttacgctgta actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat
     9841 caaaccttca gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa
     9901 aggaaaacta tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca
     9961 tgaatcattg cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa
    10021 agtggcttgt ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct
    10081 ggcccttgca cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc
    10141 tgaataagga gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg
    10201 tagaaatgga cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa
    10261 aatttgcaga acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg
    10321 aagctggtgt ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga
    10381 ttcgggtagt cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga
    10441 cttatggtca atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac
    10501 ctaaaggata tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt
    10561 gtgataatta ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag
    10621 cgattgattg gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc
    10681 cgacattaaa agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa
    10741 ggagaaattt tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta
    10801 atttcgttca ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac
    10861 tgacttatgc aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc gtagaattgg
    10921 tcgtaggaga tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag
    10981 ttgttcatta cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg
    11041 tacaaacgaa tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac
    11101 gctatcatca cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc
    11161 caggacatgg ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa
    11221 gtccatattc ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt
    11281 ttggcttacg tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg
    11341 aaaaatttat tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca aaattatatg
    11401 gcgaaggaaa gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc
    11461 tgattttgac gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag
    11521 ataataagac cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg
    11581 accatgtaaa cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg acaaaattgc
    11641 gagaagagct aggttggcag cctgagttta cgaacttccg tgatgggttg gctgacacga
    11701 tcaaatggta cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag
    11761 catatgccaa aaatggacaa taatacagag gaaatttcac tgtattaatg gaaaaacaca
    11821 tgtgaggcag gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataaccat
    11881 atgtttttat taactggagg aaatggacaa ttaggtacag aattgcgcca tttattagat
    11941 gaaaaaaatc tgccatatgt ttcaacagat gcaaaagact tggatatcac agatgctgag
    12001 aaaacaatgg cttatattac agaattaaaa cccgatgtta ttttccattg cgcagcttac
    12061 acagcagtag ataaagcaga agaagaagca aaagaattag atgagaaaat caatgtcgat
    12121 ggcacaagaa atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt
    12181 acagactatg ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg
    12241 aatccactaa atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt
    12301 ttagaagact attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc
    12361 gtcttcacga tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag
    12421 tttgggcgac caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa
    12481 aaagcaccat ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgagttc
    12541 gcaaaagaaa tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt
    12601 ccgcaaaaag caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagcgtta
    12661 ggttttgtca taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa
    12721 atgaataagt aatttccttt ttatcaaata gaatttggag catgatatgg aaaaacaaac
    12781 aagaagtgca cgacgaaaag aaaagccaaa agcgaatcag gcagattcga atttggtgct
    12841 tgacctgttt ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg tcatcgttac
    12901 ttttaattta aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc aaagagacga
    12961 tgggaaaaaa actgatgtga tttttggaca gaatcgggaa gtgcggtcag acgaatggct
    13021 ggtgcagaca cctttttacc tctcacaagc tgaagaggac ttttcgttgg tgaacgagga
    13081 ttattcatta aacggacaaa acatgatcat tgcgtataat tctcctgtaa aggatattac
    13141 ggttatcgga aaaccattca actggggatt ctttttcctc ggaagagatc gaggactttc
    13201 cttttattgg gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag tactgatgat
    13261 attaacgaag aggaaaaaag ctctatcgct aatcggtgca tttgcgctga cattttctcc
    13321 agcagtccag tggtggttca tgcaacatgt gggcgatttg attttcttta ctttgggact
    13381 gatgattgct ttttatcatt atttctacaa tcatgataaa aaatggctac gaacgttgat
    13441 gatgcttttg gtggttattt tcggtattgg gtttatttta gtgatttacc cagcccatca
    13501 agtgatgctg gcgtacttgc tgatttttta ttttgttggc ctgcttattt actatagcaa
    13561 gaaagtttca tgggatctgt tggatgtagt gttgattgga ggggcagttt tatttattgg
    13621 cggagtcatg gttcatttct ggctaacgtc aaaagacgca ttgttagctt ctttgaatac
    13681 cttatatcca ggaaaacggg taagtactgg aggaaagtgg acgattggcg aatttttttc
    13741 tttccttaca aattggaaaa ttccttttga agatatcaac tattcgaata actctgaagt
    13801 ggccctgttc tatcactttt ttccaactgt attcctagca agtccatttg tgttatttgg
    13861 caaaaaagat agcgaacaaa agttactggg acgtattttg atgatttttt gtctgttttc
    13921 tatcttttgg attaccgttg ggttgccaaa atggctagca gaaatcactc tgctgtctta
    13981 tgttcctcca ttaagagcca tccttacttt tagttttgcg gcttgtttat tatcgatctg
    14041 gtttatcgct tatatttgga gagaagatgt tattcctatt tggtataaat tcattttgtt
    14101 ggcagtaaat gcatggtctt atttctatgc gattacgcat tcgaaaatgg ataattattt
    14161 ttcggatttt gaaaccatcg cagttgctgt gttgggaaca gtgattttag cttttgcatt
    14221 atttggctgg agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg tatctggctt
    14281 tttcgtcaat ccagtcgtag aaggtacagg agcgattttt gaaaaaacac tggcaaaaga
    14341 aatccaaaaa atcgatcaac aagatcctgg taaagtttgg ttgtctgaag atgaattata
    14401 taacttcacc ccaacattgg gagtgaaatc cttcaataca gtacgctttt atccagatat
    14461 agctgcatgg aagaaaatcg atccgaaaga agaatacgag aagtattata atcggtatgc
    14521 tcatacaaga gcttttatcg ctaaggaaga tacaaagttc attttagatc gtccagatat
    14581 gttttcggtc acgttgaatt ttgaagacgc agaaaaatta gggatcgact atgtgatcag
    14641 taaacgacca ttaactgatt acaaccaatt atatgaagcg cagttcacgc agctatatgg
    14701 accggataaa gacggttata tgatttttaa agtagaatat ccggattatc cagatgtacc
    14761 acaaacacaa actcaggaaa atcagctagt cgtacagtaa taggctagct gagaataatc
    14821 ctgaaatatt agtagaaagg agagtgaaga taattcaagc aatcgtacaa aaatttgtca
    14881 acaaaatgag tgtcggtttc ttgcttttgt tgctgacgct agtctttttt gtttggacag
    14941 caggggtagg ttttccagca actttgttta tatataacaa tagttcatta cttatcgctt
    15001 ttagtgtggc tattttgctc attttaaatg tttataaagc aaaaggcgtc gattttcttt
    15061 atctaggtgt tgcgcttgcc cttttcattt tcttttctta ctttcgggaa ttaagaaatg
    15121 agtctttatt tggagactta ctgtttcctt tggttatttt attttgttta gtaattaaat
    15181 ggatcgagtg gacaaaatgg gatcgcggta tttatctgat tgtctttacg gtcttcctcg
    15241 gaatcacgtt atatcgtgta tttacagaga tccaagtacc agaaggacaa agcatctggg
    15301 ctccaggcaa ttcgctttct gatatttgga tcaatacgaa tacaatcgga agttctttga
    15361 tgactcttgc tttattgatc agtggatttg ccagttcttt tgaacgatgg tatgttcgaa
    15421 tcatcggtgg gatagcagtc gttgcagcct ttttaggtat ttgggtctgc caatctagag
    15481 gtgcattagt cgcgttgatt gtttttgcga tattggatct tttacctaaa tcgttgatgc
    15541 gtgtcattcg tgcgccattt attgcctaca cggttacgat tcttttagct ttgccgattt
    15601 cttatctggc ggctgtctct gaaaaagtca atttgtttac cgatcgagaa gatatttggc
    15661 ataaattcta tcagacgcta ggagaaaaat ctgaacaagt tttactagga atgaaaacat
    15721 ttatcttcca gcgaggaaac caatttttag gaaaccacaa tagttataat tctattttga
    15781 atatctatgg attgatagga tttgggattg ctgctttgct gcttatcctt tttattggtc
    15841 gcttaacgtt gaaagcagat ttatccaacg ggcagatgac ttttatctgg gccttttttg
    15901 cggtgatgat gcagtcattt atggaggata cattgacttc atacccatgg gttccgatta
    15961 tttatatcct gttggctatg gccgttcatc gatatgatga accagaagtt gcggaggaat
    16021 tctatgttat ggaagaggca tatgacgata tgacttatga tgatacatct tatgaggaac
    16081 aaccagatag cgctcattct tccaggatcc aacgctatca ttgataggca caaaatacga
    16141 gagaggctgt gacaaccgtc gcagcctttt ttggtttatt gcaattgtct agcatcttct
    16201 gtaaaataga aacgttagag aaaatcttgt atcatagaaa attaagaaag aaataagaac
    16261 gcagctcggt accaaaaata tggtaaaatg gcacttgact gtaattcact gaaaatcgct
    16321 tggaggaaca caaatgttta aagatttgta tttatttatg aaggacattt atcagaacag
    16381 aaaattattg ctacagtttt cgatcaatga cttcaaatca agatatgcag gatcgttttt
    16441 aggaatctta tgggcgttta tcaatccgct atttactgta ttgatttatt ggctggtatt
    16501 tggtgttgga ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg tatatttagt
    16561 taccgggatg attccctggt tcttcttctc agatgcattt gcatcaacta cggttgtttt
    16621 tcgggaatat acttatttag taaagaaagt cgtattcaat attcgtatct tgcccacgac
    16681 gaaaatccta tccaatctgt acacccattt attcttcatt ctgattggca tagctgtcgc
    16741 gcttataaat ggtatatatc cttcagcaat gtctttacag ctgatttatt atttattttg
    16801 catgtgtgct ttcttgacag gattgacttg gctgacggca tcgatccaac catttttacc
    16861 tgatatcatg caattaatta cgatcgtgct gcagttgatc atgtggacat taccgatatt
    16921 atggagccct aatcagtttg attcaggtct tgtaagcata ttgaaattaa atccgctgta
    16981 ctatgttgtt caaggttatc gtgagtcatt tttaagtgaa gcttggttct gggagcatgg
    17041 aacatatact ttgtatttct gggttttcac gttagtaatg ttcgtaatcg gctctgcagt
    17101 gttccgtaga ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc tatgacagaa
    17161 tatgcaatta gactaaaaga tatcacaaaa tcttataata tgtacgcaaa accttcggat
    17221 cgatttcgtg aagccctcaa tccttttaag aaatcctatc atgatgtatt ttatgcgttg
    17281 aaaaacgtga atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga
    17341 tcgggaaaat ctacaatgtt gaaaatcatt acgggagtac tgacaccaac atccggcacg
    17401 atggaaatcg aaggaaaaat ctcagctttg ctagaactag gatcaggatt caatcctgag
    17461 tattctggct atgaaaatat ctatttaaat ggaatggttc taggattttc aagagaagaa
    17521 gtcgatgaaa tggtcgatga tatcattgaa tttgctgaca tcggagacca tatttatcaa
    17581 cctgtaaaaa cttattcaaa cgggatgttc gttcgtctag cttttgctgt agcaatcaat
    17641 gtgaatcccg atattttgat tgtggatgaa gcattggcag taggcgatct tgaatttcaa
    17701 ctaaaatgta tggaaaaatt tacagaaatc aagaactcgg gaaagacgat tttattcgtt
    17761 tctcatgata tcagttcgat tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga
    17821 gaagtcgtcg aatatggcga tacgatggaa gtaacgacga attatgaaaa ctttttaaag
    17881 aaaaaatcag ttaaaacagt ggatcggaat aaaaaccaag tagaacggtt cgccgctcca
    17941 gatatcgtcg acgtgacgaa agcggaattg ttggataaag acaaaaatcc ggtggatatg
    18001 ctggaacaag gagaaaaact atacgttaaa gtaacttatg aagtaaaaga tgatacaatc
    18061 aaaaatccgg ttttaggaat tgcgctacga acagtcgaca atttttatgt atgcggactt
    18121 aatactctac tagatggtat caagattcct tggaaaaaag gagagaatat cttttatctg
    18181 gagtatgaaa agttaggttt gttagctggt gaatattatt ttgacgttgc ggtctttgaa
    18241 gaaaatgcaa cggttccttt ggtatataag acaaagtaca tgaacttgtt tgtcagcggt
    18301 tcttatatcg gagaaggcat cgtggtctta gaccataaat gggaagaagg gactcatagc
    18361 aatgaaatat gattttgaaa tggacctaga tgaacaaagc agtgtgggaa aaattgccgc
    18421 acagatcaaa ccaggaagta aagttttaga gtttggtccc ggaaacggcc gattaacgaa
    18481 acatttaatc ggagcaaaac aatgtgaagt gagcattgtt gagttagata aggaattgtt
    18541 cgattttgtc agcgaatttg ctcaagatgg attttatggt gatatcgaaa gttttgagtg
    18601 ggcaaactat tatgctggac agacatttga ttacgtcttg tttgcggatg ttttagaaca
    18661 tttggtagat cctggaaaaa cattgaagaa agtcagagaa tttttgaatg aagaaggaga
    18721 gatcctgatt acattcccta acttggcgca taattctgtc atgatcgatt tgttcaataa
    18781 ccaattgccg tgggcttctt atggtctcct agatgaaacc cataattctt tttatactca
    18841 tgatggcttc caaaaagttt ttgaaaaagc gggattattc attaatattg aagattattt
    18901 gtacttagct gtaggtgata cagaattaaa atcaacttat gaagaattgc cggaagctgt
    18961 acgctatgat ttcaaaatgc gaccatttgg tgaagtctac caatactttt tctcattgat
    19021 gaaacatcca gtagcacaaa gttccattgc agagccacaa aattctaatt atgtaaaagt
    19081 gttagaagta acccagcaaa cgaaacaaga tgaaacaatc caacaaatac cattcaataa
    19141 tttcacaggg gaaaatgaga cactaagttt ccccgtatca gaaacaacag aaagaatggt
    19201 gtttcgcttc gcgcaacaac ctagctttat cgaattcagc gcagaagctg ccggagaaaa
    19261 actaacattt atcgattcga atgctgtagt caaaacagta aatgattgtt atttgtttga
    19321 tgggaaagaa ttaccagagt tcgttctaac ggatatttct gggaaagaag tgactatcca
    19381 ttgtcactat cgctttatcg gggaattgac accaacaatg aaagaattgt tggaaacgat
    19441 tcgtcccatg gctgaagtca caaagcagct ttctgagaaa aatgacgaat tagaaagaga
    19501 aaatcatcat cttcttgagg aaaatacaag actagaccat acgttaaaaa caaccacaaa
    19561 tcgctattgc acattgttgg aaagtgatga atggacaata aaacatagac taggacgacg
    19621 taagaaagaa acttcgaaaa aaatccaaga aaaagaactt tctatatgta ttgacgagaa
    19681 aatctgggat gcagaaacaa aaatattaaa aatcatcgga tggggaatcg ctaattcgga
    19741 tcgccagccc ttgtcctata agttatcagc tgaccaatcg ccatttttcg aagcaattcc
    19801 tgtttcacga gaagaagtca atcaagcaga acagctcgca aaaggaacag aagcgggatt
    19861 tgagttacga atcctttgcg agcaagaacg ttcattctta gtggaagcag tagctcaaaa
    19921 tggccaatca tggatcattg aaatgtaaat aatgattaac ttataaagat aaggagtaaa
    19981 cgaatcgtgc aaaaaaaaga aattgccgta tttatcgatc aaatcactcg tgaacgggcc
    20041 actggcgatt tgcttattgt gggctgggca attgatgaag tgaccaaaga aatccctacg
    20101 ataaaggtag aaaaagaaaa tgtgattgct gaagcaacac atgtcgtaag attggatatc
    20161 aatcatttat ataatcttga tgttaagacc caaagcggtt tcaaggtacg tttatcaggg
    20221 aaaatgagag gaaaagccat cttagacttc caaacagcga gacatcaaaa tggcatagct
    20281 gtgaaattaa acggtaaata cccttatgat gacggcattg aatcttcatg ggaaagaaaa
    20341 aaaagattac tgaaaaaagg gataaattat gcaaggactc atggcgtaaa aaaagcgatc
    20401 cgtcgggtca aattggaatt gaagccagga tcgatcgact atgcagagtg gatcagtcgt
    20461 catgagaaac cagatttgaa agagcaaagg aaagaagcac aaggatttgc gtaccgtcca
    20521 ttgatttcca tacttatgcc tgtctataac gttgaaatca aatggctcga aaaatgcatt
    20581 gattcggtac tggatcaaac gtatgatcat tgggaactat gtatcagtga tgatgcttct
    20641 actgatccag ctatccgaaa gtgtctggaa tcctatcaag caaaagatga tagaatcaaa
    20701 gtcgttttcc gaaaagaaaa tggacacatc agtctggcaa ctaattctgc attggaaata
    20761 gcagaaggag aatttatagc actcttagat aatgatgatg aattaccgcc atttgcatta
    20821 tatgaagtgg caaaagtgct aaacgtacat ccagaactgg atgtgatcta cagtgacgaa
    20881 gataaaatcg atgcagatgg caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct
    20941 gatacattga tgggaaataa ttatatctct cacttaggcg tatatcgaac aagcattgtt
    21001 aaagaacttg gcggtttccg taaaggctat gaaggttctc aagattatga tttggtcctt
    21061 cgtgtgacag aacaaatccc tgcagatcat atttaccaca ttgaccgagt cttgtatcat
    21121 tggcggacga tcccaggatc aactgcaagt aatggcgaag ccaaaagtta tatctatgat
    21181 tccggagtga aagcattgac agatgccctg tctagaagaa acatcaaagg aagcgtacgt
    21241 cctggcagaa tttctggatt ttatgagatt gcttatgacg tcttgcaaga ggatttggtc
    21301 agtgtgatca ttccaacgaa gaatggctat gaagatctaa aaacttgtgt ggattcgatc
    21361 atcgaaaaaa cgtcttattc aaattatgaa atcattattg cggataacgg aagtacagat
    21421 ccaaaaatgc aagaattatt tgcagaatac aagcatcagc tgaaagaccg ctttatcgta
    21481 gaattgattg acattccatt caattattct cgaattaaca atttagcagc agaaaaagca
    21541 aacggcaaat actttttatt cttgaataac gatacagaag tgattgagcc agattggatg
    21601 acggtgatgg tttcttatgc acagtttgat cgaatcggct gtgtaggagc aaaactgttt
    21661 tatccagatg atacaacgca gcatgcagga gtattattag gaatcggtgg tgttgcaggg
    21721 catgcattga ataactatga tcgaacacat tgtggttatt tcggccgatt ggtcattgac
    21781 gtcaattact tagcagtcac tgcagcttgt atgatggtca aggcagctga cttcaatgct
    21841 gttaacgggt ttgatgaaac attagaagta gcattcaacg atgttgactt gtgcttgaaa
    21901 gtttatgaac tgggtcgtta caatgtctat gcacatcaag cagaattgta ccatttcgaa
    21961 tctaaatcaa gaggttatga agatactcca gaaaaacaaa gacgttttgc tggtgaaatc
    22021 aaaaaaatgc aagataaatg gccagcatat attgcgcatg atccatttta caatgataat
    22081 ttaacaaaag acggaatcgg cgacttttcg ttgagaccag attaaactat aataaaaagc
    22141 tgttatgttg tgattcgctc gcacataacg gctttttata gttttttgtc aaaaaaaggc
    22201 agaaggtgcc gactcaaaaa aataagtcga ggttgggaca gaagtgttta actccgagaa
    22261 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcggc ttatttaaga
    22321 agaagttgct tctgctccca ccgtttattc gtttttaaag aatgagacaa aagtgtttct
    22381 tacttttgtc ccacgctctt attcatgctc agagcgccaa tagatacaaa atcatagcca
    22441 aagtttcacg aaccctagct gtcaaagaat cgatatttta ttttaatata ggctatttta
    22501 aaataaatgg tttaaggtat tcctctacaa aagccactct atcaattatc atgggtttaa
    22561 aataagaagt gatggagata accagattgt tcttagcgtc aatataaaga acatttccac
    22621 tattaccaat tgctgaatat atttcgttct cagaatctaa tacccaccat aaatagccat
    22681 atctcatatt ttgtaagtct tctaaagcac tggttctagg tttgaacatc tcgtttatcc
    22741 acatgggtgg tacgatttgt tttcctttat acatcccttt atttaggcat aatacaccaa
    22801 tttttgccag atcacttgcc gataaacaca aaccatatcc tgccgtcccg atatttagtg
    22861 gatcacaaaa ccagacattg gttttaggag acttatccat tgtaaactgc ttatgttctt
    22921 cagctgtttc agcataataa ttgacatggt ttggtatccc tataggatca aatagatact
    22981 tattggcaaa atcaacagtt gttagcttac ttgtctgata caacaagcca gttaatatgt
    23041 gtaaacagac agtttgatag ttgaattcgc cggttatacc ttttcttcct cccaataaat
    23101 ctagtgatgt ttttgtccaa tcttcacttg aacagacttt tgtccatgga tcgcctttac
    23161 atttgtaggg tgctttcatt gttagcaagt gcttgattgt aatagcgtaa atagttttct
    23221 ccccgcgttt cactttatac tctggaaaat aatctaatac tttatcatcg atacttttga
    23281 ttaatccttg ctctaaagca atgccaatca gcaaagatac aatacttttc gttgctgaca
    23341 tgatatgaca agtatcgtct tttttataac cattccattc atctgtataa acttctaccc
    23401 catccttata tgcgacaatt tgacatatat ttggttgtcg aaaaccaata aaatcatgca
    23461 actcttgttt attcataaga tattacctca ctttcttcta taagcataat gtcattaaaa
    23521 atcgatttca attttttcga ttcttcacgt aatacaggta ttcttatgga ctagacagcg
    23581 ctttgggaag atggattctc tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa
    23641 gcagcaaata cttcgacttc tttaatttac caacattagt gaaaatcgat tcatctggag
    23701 atttgtaatt tagagtcctt gtcttgatta gaggtgtata aagcttttta ttagacattt
    23761 ttaaattcca ttacaatttg cttataaact aggtttttaa aggtgactat ggtaagataa
    23821 tagagatatt attttgtgat taggagtgta tatatgaata aaaatggaga atggagtgct
    23881 gcacgacgga tattcattat attaatggat gttattgtat acaatgcagc aatatatagt
    23941 agtttcttac tgaagtttcg tggagagatc ccattacgaa atttagaaac tttccaacac
    24001 tcagcaatta ttatttcagt gatttttatt gcgttgaaca ttttgttggg tgcatatgtt
    24061 ttttataatc gaatgatcag tgacatcgtg tttgtcacag tcatcggtca ggtattgatg
    24121 acactaggga tcatggtagt gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt
    24181 atcttacttt catttgttct cggtacgctt ttcttaagta tctatcgtat tctcgtatat
    24241 aaactttatt tacgcgtaag tcgagataaa aaggtcgtca ttattgggtt agaaaaggat
    24301 gtcgcaccag caatccaaaa ttttggttca aagaaaaaca acaaacataa agtcgaagca
    24361 gtcattcttg ataactatta tgcaaacatc aaaaatatga ttaaagacat cgatattgtc
    24421 tatcttgctt ctcatatcga agaaactgaa aaaatcaaaa tctatcaatt agttactaaa
    24481 aatgaaaaaa aattgttttt gaatacaaca tttgaaaact tgacattgat caatccaaat
    24541 attatgaatt tcgaagatga aagtattatt gaagcatctg gatttagaat ccaacctgaa
    24601 tatgaactat tcaaacgttt gtttgatatc atcgtttcac ttgttttatt agttattgct
    24661 tcaccattta tgttattcac agcaatttta gtcaaagtga catcaccggg accaatcatc
    24721 tataaacaag tgcggatcac aaaaaatcaa aaggaatttt caatctataa attccgctcg
    24781 atgaccgcca cagccgaagc aaaatctgga ccagttttag ctaagagcaa tgatgcacga
    24841 gtaacgcctg taggtaaatt tattcgagct gtccgctttg atgaattacc acaaatcttc
    24901 aatgtgctga aaggcgatat gtcgatcgta ggaccacgtc ctgaacgttc tttcttcgtc
    24961 gatcagttca atgcagaaaa cccttattac tacttacggc ataatgttcg tgctggtatc
    25021 actgggtatg cacaagtcta tggaaaatat gtctctgatt acaacagtaa attacgtttt
    25081 gatttacttt atatcaaaaa atattcattg atgcttgatt taaagatctt gctgcaaacc
    25141 atcaagatcc tatttgataa aatgtcctct caaggacttg aagaagacga aaatgtgatc
    25201 tcattggcag aaattgcttt tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa
    25261 atgaacaatc cgaaattcag cataatcgta ccagtataca atgcagaagc aactattggt
    25321 cgaacgattg atattctaaa agaattggat tatgaagagt acgaagtcgt tttagtaaat
    25381 gatggatcaa cagatcaaac acaggaaatc attcaagaac tgattgaaaa tgatgaacgt
    25441 tttgaattga ttactacgac caatcaaggg cccggattag caagaaacga aggaataaga
    25501 cagtcaaaag gagaatatct cttattcttc gatgcagatg acacgccagt aaaaacgatc
    25561 ttgtcagatt atgatcattt gctagaaaag agtccaaaag cggatcttgt tgttagttcg
    25621 tttgtttttc gtacaatgga cgagggaaga atcgtttccg aaaaagaaaa cttagttaca
    25681 gaatgtcact atctaactca cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa
    25741 cagttgatgt atgttgtatg gaacaaatgc tatcgtagcg atattctgaa aaataaccaa
    25801 attctattta aaacttataa tagttgtgag gatcgaatat ttaacttaca ttattatgaa
    25861 tactgtcatc aagtgatcat gaacccaaaa gtagaatata tttatgaatt tgaaggtggg
    25921 aaggggatca ctaatcaata ccgacctaac aagttcgaaa catttaaaga attctattca
    25981 ttagcaaata ctgtaacaga cgatattaat aaagacggta tggcggcgct tcttttaaaa
    26041 gggacaactt cagttatttt ttctatttat agtacctctg caagaacttc aaaagagaag
    26101 aaagacgaag caaagcagat attgagtgat ccagtgatcc gagaagccaa gcaaattgct
    26161 aagacggatt cttcagcaaa aaaaataaca aagttgcttt acaatatgcc agctccacta
    26221 tttttatctg ctgtaaaagt tggaagcttc gttgaagtga aaatgccagg gttaatggct
    26281 gctttgaagc gagtatatta gttaatggag gaaaatataa tgaaaagaat tttgcatttt
    26341 cagggacgaa tgggtttagg tggagcagag tctttcatga tgaatttata tcgtaaaatt
    26401 gatcgtacaa actatcaatt tgattttttg atttatgaag attatgcaga tgttcaagac
    26461 tatcattcag agattgaaag attaggaggc cgtatttttg ttgtacctaa tcctaagaaa
    26521 aatattttta aatatttaat agaagtgaat aagttactaa aaaaagaatc tttttctata
    26581 gttcacaatc aggtttattt tggcggggga attaatttat ggttagccaa aaagaatgga
    26641 atcagacaga gaattgctca tagccatgca actgaagatg gaaaatctca aaatattgta
    26701 atgaatgtat tgcgtaaatt tttaaccaaa ttgttgttac aaaatgcaac agactacctt
    26761 gccgttagtc aagaagctgg tgaaagtctg tttcaaaacc atccctttga gatagttcat
    26821 aatggtattg atttagagct ttatagtaaa aattcagaag ctaaggttaa taagagaaaa
    26881 gaattggata tctcaatgag tacttttgtt gtgggaaaca ttggacgatt agagaaacaa
    26941 aaaaatcaat cttatttgtt agagattttt ttagaaatac taaaaatgaa ttctgattct
    27001 tacttgattt tagtaggtga agggtcactt agaaataaac tgaaagccaa agcagtagaa
    27061 ttggatatag agaaaagagt aaaattttta ggagaaagaa acgatattcc cgagttatta
    27121 tctgtgatgg atgtttttgt aatgccttct ctttatgaag gtctgccaat ggttggatta
    27181 gaagcacaag cagcaaaatt aaaattagtc ttagcagata ctatttccaa agatacaaag
    27241 ttaacaaata atgtggattt tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa
    27301 gtaataaaag agccgtatgc taataaaata acacaagaac taaagagtta tgatgtttcg
    27361 tacactactg aacaaatgga gagtatatat agtagagatc attaaaaatg ttcaagatat
    27421 tttaggagtg attatttttg aaaatatgtt atttgatatt ggcatacaat gatattggca
    27481 acttaaagag attggtcaat cgattgaacg aaaatgcagt ttttattatt catatagata
    27541 aaaaaactga tatagaacca tttattgaag catttaaatc atatgataat gttgaatttt
    27601 tatgtaataa aaaccgtgtt aaaatttatt ggggtggttt ttccattgta caagcagaaa
    27661 tcaatttagt aaagagagca ttacaaaatg agaaatattt aaaatatgta ttattatctg
    27721 gtgctgatta tccaatcaaa gataatgagt atatttataa ttattttaaa aagaatagtt
    27781 cagtggagtt tattcgtgga atagatctag atcaaattaa acataaagaa ttttattata
    27841 aacatataga tgtatatcaa aaacatgatt atcctagaat taataggaat aacacaactg
    27901 catttaaaat ttttagagct ataattaatc gttgtctgag gatgattaaa cttcccccaa
    27961 aaattagaca ccataaattt gatttgtatc atggttctca atggtgggct ttgtcgaaag
    28021 aatgcctaac agaattaata caaatgtatg aacaaaatca acaagactac ctgaatttta
    28081 aaataggtat gtttgcccca gatgaaaaat tttttcatac actttttttt aattcctcgt
    28141 tcaaaaataa aaatgttatt ggaggaccag atttaccact tgaattgaaa aatattgaag
    28201 aaactacttt acaaacttcg aaattagcaa atattcatat tattgatcct tccatgaaca
    28261 aatggtttga tgaaagggat atagatcaaa taaaagaaag tgaaaaatta tttgtaagaa
    28321 aagttaaatc ggattactct gataaactat taaatagaat tgataaagaa attttgaaag
    28381 ttggaaatat agatgaatag aatagtacct gttttagaga agagtttatt aagtcgagat
    28441 gcgtttcgag taataaattt aatcatttgt ctgctttatt ttatgcctag tacttttaat
    28501 gtaactaata tcccgatgaa aatagctttt gcttggggtg gaattatagt tttgtatgag
    28561 gtttttataa agaaaaattt ttttaaaatg cgtttttctt ggctactgat tggagcaatg
    28621 attagtttta tcttaactat aatcattaac agagaaaatt ttttaattcc atctatttat
    28681 aattttggat atttgctgat tactttgata gtaatttttc ctgcagactt ttcacaaagt
    28741 gaagaggaaa aaaagaaaaa aatggtgtat tttaataatg ttttcattat cataattttt
    28801 ttagctgcgc taatatctat ctatcaattt atttttttga tttcttatca tgtcccaaca
    28861 ggaacgccag gattacttgc aagacaaggt tttattgaac atcggttatt tggtgtctat
    28921 acaagtccaa atgttggagc tatgtttgga tatacttcta ttattttaag tttaattagt
    28981 gtattaattt caaaaataac aaaaacgatt cgtgtttttt atatgatgaa ttttatcgta
    29041 caaatatttt atattacact ttcttcgtct agaggtgccc aggtagtaat ttgttctttt
    29101 ttagtattat ttcttttgtt gtttgccaat tcaaaattta gaaagagcat tagagaagta
    29161 ctacctgtaa aaatcaaatg gatggtaagc atattattag ttctattggt atatttttct
    29221 tttggtacta attatgctaa ggatttttta gcaacaattc ctgtatcgat tgatagaaaa
    29281 gtagaagtta tagagtcaaa tgaatcagat aaaactccac ttccagaaaa agaagtaata
    29341 ttacagcatt ctagtgataa ctccgaaatt tcagctggtc gttttacgat ttggaaagct
    29401 gcgcttaaag ttataagacc aactttgtta tttggttatg gagagaccga tttctatggg
    29461 aatagcgatg caaaattttt gaaaaaagta gatcttgata ctacagatgt gaatgaacta
    29521 aaaagagcac atggaaacat gcataatgga tttcttcaag tacttgtatc aagtggtata
    29581 attgctttta tatgtatcta tgctttctat gtgttaaata tctttagttt actcaaaaca
    29641 ttttttagaa gtaaagtgaa ttatgcgttt cttggtattg ttttaatttt tatactatct
    29701 atttttatag atgaaatggt ggaggctcat gtacttttca ataagcgcga tgtcatttca
    29761 attgttttct ggtattattt gggaagcatt tcttacttgt atttaaaaaa ggagaattaa
    29821 tatgaagaca gctttcattg cttggactcc tttacatttg attaatatat taaatgttta
    29881 tcaaaatttt taccgagaat caaaaggaga tttatttatt tattctgaat ttgaaaacgc
    29941 tagaagatat agtgagcgta taaaagaatt agggctattt aatgatgttg tttttgtgaa
    30001 tcaccggaaa atcggttctt cctttaaacg taaaattaac ttgttaacta accataatca
    30061 atttttctcg gatgatttat ccgtttatga tgaaattttt atacaggggg aaaattattt
    30121 cacaaaaata cttttctcca atgtttacaa aaaaaatcca gatgtaaaac taaattatat
    30181 agaagatggt ttgggtactt atttgaagaa gagtatattt tcaacaagta aaaacgttca
    30241 aagagttttt aaaatattaa ataaaaatag catcttcaaa aaaaaaattg acgattattt
    30301 tgtctattgt ccggaattgt tactttatga tgtaaaaaat ataaaaaaaa ttcccaaaat
    30361 agatgaaaac agtaaattga tgaaaaacat acaatcaatc tttgggatag aagaagtttt
    30421 tgtcggagat aaatgtatct tttttgatca acctttatta tctgatggtt atggcataga
    30481 tgaaacagat attttcgcta aagtaaatag tatattttct ggaaaaaaag aaactttaat
    30541 taagcttcat cctagagcaa ggacaaatcg ttatggtgaa aaaaacatat tgaattccag
    30601 tttaccgttt gaagtcttgc tacttgaaaa tgacttctct tcaacacttt tgattagtcc
    30661 tatatctaca atttcttttt caccatctat gatgtttgaa aagacgatca actctattat
    30721 gttggctaaa ttaattttaa atgaatatac agcattatct gaagataaaa aagaaacatt
    30781 gacaaatatt gttcttttct gtgaacatta taaccgtcta tcagaagaaa aaattttact
    30841 cccagaaaac tgggcagaat taagaaagtt ggtagagtat gggattattt aacacacttt
    30901 ccaagtcaaa attagttaag tccagtttct ggtatactat agggaatttt tttattaagg
    30961 gaatttcatt cataactatt cctatttttg taaatattac tactcaagca gaatatggtt
    31021 tgattaataa tttcacttca tttgtatcaa ttttttcgat tcttgttggg ctaagcctaa
    31081 attcggcaat taataatgca agttttgact ataaagatga cataaatgaa tttatgtcat
    31141 cggtactttc tctagctaca ctttcgttaa ttgtgttctt attaataggg aacatatatt
    31201 ttttgttttc aaatgctttt tttgatatga accaacttgt atttaatttg atgttattac
    31261 aaagttttgc tagttttgta attagttata tccagtctta ttttacgatt aactttaacc
    31321 attttaaata tttgatttta tcttttatat ctactttttt gaatattgca atctctgtag
    31381 ctttgatgca gacattatat tttgatgata aatatgttgg cagagtagtc ggcggaacac
    31441 tgtcatttgt gttgataggt ttagtaattt atttaagaat aatgataaag ggtgctcatt
    31501 tttttgataa aaaacagtgg agatacgctt taaaaatctc tttgccgcta atcccacatt
    31561 ccttagcaaa tatagtttta gcacaattcg atagaattat gattaattct tatttaggtc
    31621 ctgctgcggg gggaatatat tcctacatta gtaacatagg tattattcta agcgtaatat
    31681 ggatgtctac taataacgcc tgggttccat ggttctatgg tgagatgagt aaaaataatg
    31741 aaaaaaaagt gaagaaagtt agtaactatt atttagtagt ctttactttg attacgatga
    31801 ttgtaatggt agtttcgatt gatttagcaa aatttatggc tccgaaagac tattattctg
    31861 ggttatcttt agtggttccg ataaccttag gctatttctt tcagttttta tatagtttac
    31921 cagttaatgc tgaattttat gagaaaaaaa catcgtttat tgcgattggt acagtagcaa
    31981 gtgctatagt taatatacta ttaaacatga ttttcatacc taggttagga ataattgctg
    32041 ctggatacac aaccgtattt acgtattttt tactttttat ttttcattat aatatcgcta
    32101 aaaaaattac gaataaacaa ttatttgaca caaaaaaaat atgtatcata ataatttttg
    32161 tatcaatttt ttctttgata cttcatattt taatcaatca aatgttgatt cgttacgttt
    32221 tgacatcatt attgataatt agtttagtta tatatttttt aaagattaag aatcgaatta
    32281 tagatgacta taattcatag gaggagatat aagtgtttaa aggaaaaaca atattagtaa
    32341 ctggcggaac tggttccatt ggaagtgaaa ttgttcgtca gcttttaaaa tatgaaccaa
    32401 aagctatcag aatttatagt agaggtgaag aaaaacaatt ttatatgcaa caagagctct
    32461 caaatcacac taatttgaga tttttaattg gtgatatcag agactctcaa aggattgatt
    32521 atgcatgtcg tgatgtggat attatttttc atgctgccgc aatgaaacac gttccagcaa
    32581 gtgaatataa tcctatggaa gctgtaaaaa caaatgtgat gggaactcaa aatgtaatag
    32641 atgcagcgat caaaaataat gtaaaacatt ttgtaggtat cagtacagat aaagtagtga
    32701 gtcctactaa caccatgggt gcgacaaagt tattgagtga aaaattaata atagctgcca
    32761 atcagtataa aggaaaaaca gaaacaatat tttcttgtgt aaggtttgga aatgtcatgg
    32821 ggtcaagtgg atctgttatt cctcttttta gtaaacaaat aaaaaacaat aaagatatta
    32881 ctttgacaga tgttgatatg acacgattta tgatgtcaat accggatgct gcaagattag
    32941 ttctgtctgc tgctatgaat tcaaaatttg gagaaacata tgtcttaaaa atgccggcaa
    33001 tcaatatttt aactttagct caatcattaa taaaaatata taatgataca catgatgaaa
    33061 aatatagagg tgaaataaaa gttgtaggta tcaggccagg agaaaaatta tatgaagagc
    33121 taatgactac agacgaaatt gaacgctcat atgaaaatga taaaatgtat atcattccac
    33181 cacactatca tgctactgac gaactgtaca aagattatga aaaaattaaa atcactgcat
    33241 actcttcaga tgcaacagaa ttaatgagtg gacaggaatt tattagaatc gcaaaagaga
    33301 ataatgtcgt gacttggtaa ggagttttat gatgaaatca gaagtatatg taattgcaga
    33361 aattggtgta aaccataatg gaagtctaga tttagcttta gaaagtattg ataaagctaa
    33421 agaatgtgga gctaacgcgg ttaaatttca aacatttaaa acgaataagc tagtttccaa
    33481 aaaggctcca atggcaaaat atcagaagga taatcttaaa gaggacagtt ctcaatttga
    33541 aatgctaaaa aaattagaat tatctcaatc tgattttatt agaataaaag attattgtat
    33601 acaaaataaa attgattttt tgtctactcc ttttgatgaa gaaagtgcag actttctaaa
    33661 acaaattggt gtacaaggat ttaaaattgg ctcaggagat ttaacaaatt taccttttct
    33721 tagaaaaata gatatgtatg ggttgccagt tttattatct acaggtatgg ctgatattca
    33781 tgaggtcagt gaggctgtag attgtttcat agatagtcca gttactattt tacattgtac
    33841 ttcaaattat ccggctagtt ctgaagatat taatttattg gcgattaaca cattaaaaga
    33901 aatgttcggg atacccgttg ggtactccga tcattcattg ggatatgatg ttggtatttg
    33961 tgctgttgcg atgggtgcta aggtaattga gaaacatttt accttggata aggaactacc
    34021 tggtccagat cataaagctt ctttagatcc tcaggaattt gcagattttg taaagcatat
    34081 tagaaacact gaaatatttt taggtgatgg tgttaagcga gcaatgcctt ctgaacttag
    34141 tacaaaagaa gtatcaagaa aaagcctagt ggtcactgag aatttaaatg tgggagatat
    34201 tcttacagag aaatctctta caataaaacg tcctggaaat ggaatagctc ctaagttttt
    34261 atatcaatat attggcaaaa aagttaaaag accaataagt gcagatagtg ttctgcttga
    34321 ggaagatgtt gaatgaaaaa aatacttata gtaagtggaa cacgagctga ttatggaata
    34381 tataaacctg ttgctgagaa tttactacaa gataattggg atgtatcatt tgttgtttct
    34441 ggaatgcatt tatcccataa atatggattt actaaagaat tgatcttagc tgacggattt
    34501 agaattagtg gagaagttca aagtttgttt ttagagaaca ataagggaaa catggctcgg
    34561 tctgtggcat tagagataaa tggtttcaca aatatatttg aacaaactag ccctgatttt
    34621 gtattattac taggggatag aggagaaatg ttggcagctg cgatagcttg tttgtatctt
    34681 ggaatcaaaa cagcacatat tcacggaggc gaagtgtctg gtactgtaga tgaatcaata
    34741 agacatgcca taacgaaaat agcatctgtt cattttgctg ctacggaaaa aagcaaagag
    34801 cgtttaataa aaatggggga agattcttgg agagtttttc ccgtcggagc tcctagaata
    34861 gatactattt taaataatag attacctagt tttagagata taaaagaaaa atatcatcta
    34921 aaatttgaaa agaaaaaata cgttttgcaa gtatttcatc cagttgtaac cgaactagaa
    34981 aatatagaaa aacaaatttt agctttgatc aattcaatgg aacaacaatc tcaaccaatt
    35041 atttgcattt tacctaatag tgatgctggt agcgaaatta tcagaaaaat gtaccagaaa
    35101 tatttaaaaa cggatattat ttatattacg aatttagagc cggaggaata cctgactata
    35161 ttaaaagagt gcaaatttat gatcggaaat tcttcttctg ggataattga agctgcatca
    35221 ttcaaaatac cagtaataaa tttaggaacc agacaaaatg gacgagaaca atctaaaaat
    35281 attataaatg caaatctaaa tgaaaatgaa attgaaactg ctttgaataa aatacaagat
    35341 cctcagttta taaaaagctt gaaaaatgtt aagaacatct acggtgacgg caacagttct
    35401 agaagaataa caaaaacatt gaatcaaata ttgaatgatg gggaagatat taagtggatt
    35461 caaaaacaaa tagcttatta aaatatacta gtgatccata cattattatt ggggctggcg
    35521 gacacgcaaa agtcattatt gaaaccttaa gattagaaaa taagttaata tatggatttt
    35581 ttgatgataa gcaaaataaa atgcaagatc tgaaatatct tgggaaaata aacgaattga
    35641 atagctgggc ggatagcaaa aaaaaaatct ataaatttat tgtagctatt ggtgactgtg
    35701 tagtaagaaa aaagattata aaaaaattga atatgccaag tgagcaatat ggaatagctg
    35761 ttcatccaag tgctatagtg gcaaaagaaa tttcaattgg ttatggaact gttatttttg
    35821 cgaatgctgt aattaaccca gatgctgtaa ttggagagca tgctataata aacacaggta
    35881 gcattgttga gcatgattgt agaattaata actatgttca tttatctcca ggtgtatgct
    35941 tagcgggtgg agtacatgta ggtgtaggaa ctcaaatagg aattggttct caatgtatcc
    36001 agatgaagaa aattggatct tggtgtatga taggcgcagg gagcactatt gttaaggata
    36061 ttccctcaca ttccttagct tatggcaatc cagctaaaat taagaaagaa ggaattaatt
    36121 ttgaattcta gaattttatt atcaagtcct catatgggag gaacagaaga aaaatatgtt
    36181 gctaaggctt ttaaagataa ttggatagcg ccattaggtg aaaatgtgga taaatttgaa
    36241 gaaagtatta caaaatatac tggttctaag ggcggagctt ctgcgttaag ttcaggtaca
    36301 gctgccatac atcttgcctt aagactgctt aatgttggaa aaaacgatat tgtctttgct
    36361 ccttcattta cttttatagc tacagtaaat cctattttat atcaaggggc tgtaccagtg
    36421 ttcattgact cagaacctaa tacatggaat atgagccctg ttgcactaga gagcgcatta
    36481 gaagaagctg aaagaaacaa ccaattacct aaggcaatca tcgtagtgaa tttatatggt
    36541 caaatggctg attatgaacg aattttagct attgcaaacc aatttaatgt tccagtgata
    36601 gaagatgcag cggaatcgtt aggttctact tataagggaa tttcgtcagg cttatttggg
    36661 acaattggga tctattcatt taacggtaat aagatcatca caacctcagg tggaggagct
    36721 ctagtgtcta gtgataaaaa atatgttgag cattcaagat ttttagcaac acaagcaaga
    36781 gatcaagcac cgtattatca acatagcgaa ataggttata attaccgtct aagcaatatt
    36841 tctgccggta tcggacgtgg acaaatggaa gtattgaatg atagagtagc taaacgaaga
    36901 gaaattttca atcgatataa ggaagctttt tctggaattg atagaattga aatgatgcca
    36961 gaagtggatg gcatggtgcc aaatcgttgg ttgtctactt taacgatcaa tgattataat
    37021 tctattgatc aagtagttaa cgtaataaat aaactaaatg aaaaaaacat tgaaacaaga
    37081 gctttgtgga aaccactaca tttgcaacca gtatttcaag gggtaaaaat gtattaccat
    37141 gataataatc ttagtgtgtc agaaaagtta tttaatagcg gcttatgctt accttctgga
    37201 agtaatatga cagatagtga acaagaattt gttattgatg agtttaaaaa aatattcaag
    37261 taggtgtgat attttatgaa cataggaatt attggtttag gttccatggg gaaaagaaga
    37321 cttagactca ttcaagaaca ctttaatgat attttactat gtggtgttga tactaatgag
    37381 gcgagacgaa aggaagttga aaaggaattt ggaataccaa cttataatag tctaacagag
    37441 gcaaaaaatt tgtttgaaat aaattcagtt tttgtttgta catcccctat aacccatgaa
    37501 tttattataa aagaggcttt aaatttagat tgtaatatct ttacggaaat caatttatta
    37561 aatgaatatt atgatgaaat aatcgaagta gctaaaaaga aagatagatt actttatcta
    37621 tcttctacat ttttgaagag aaaagaaatt caatatatta agtctaaagt gacgcataat
    37681 aaaaaaattt cttatcaata ccatgtagga cagtatttac ccgactggca tccttgggaa
    37741 gattatcgca atttttttgt ttcaaacaaa aaaactaatg ggtgtagaga aatattcgct
    37801 attgaattac cttggattat agatacattt gggaaagtta aatcgtttac tgtaatgacc
    37861 cagaaattat ccaatcttga tattgattat tcggacagtt tcactcttat tattgagcat
    37921 gaaactggta tagttggaac tttaagtgta gatgttgttt caagagtagc taaaagaaat
    37981 ttaactataa ttggtgaaaa gattttaata gaatgggaag gtaacccgga ttctctattt
    38041 tcatggaatg aagaaacttc tagtatgaca gatattcaat tgtacgatga ggttatgcat
    38101 gataataatt attcacatac tattattgaa gatgcatatt tagaggagat taaagaattt
    38161 ttcacgcttt tggaaaagaa aatggaaccc caatatactt ttgaaaaaga taagtatatt
    38221 attaacttaa ttaacgacat tgaggtaaat taatgatgaa agtaattttt tttgggttag
    38281 ggtcaattgg aaagagacat ctaaaaaatc taataagcta tggaaaaaag aattctatta
    38341 attttgatat aacagcttat agtagcagtg gaaaaaaaga gtttgaagaa ataacgtata
    38401 ttcaaaatag agacgatttg gatacacatt atgatatagc ttttattaca aatccgactt
    38461 ttaagcatct ggaaacacta aatgtaattg ttgaaaaagt tgattacata tttttagaaa
    38521 aacctgcttt ttctgagagt attgatctta ctcaatataa ttatgattac aaaaatattt
    38581 atgttgctgc tccactgagg tataaaaata tcatggaagt tgttcgtaat agtattcaaa
    38641 gtaaaaaagt atattctgta agagctattt gttctactta tcttcctgat tggagaaaag
    38701 gtactgatta tagaaataat tattctgcct ttaaatctat ggggggagga gtagaattag
    38761 actgtataca tgaattggat tatattataa acctatttgg tttccctaaa tcagtaaaaa
    38821 aacagtttgg taaatattcg aatcttgaaa ttaacagtaa tgatttagca acatatatat
    38881 tgagatacga tgataaaatt atagaaattc atttagatta ttttggaaga gttcctacaa
    38941 gaaaatttca attgtttact ttagatgaat gtatcaatgt tgacttatta aataataaaa
    39001 taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat atgtatgaga
    39061 aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg aatgatttat
    39121 atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt ttatttacta
    39181 tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat ttggcaggaa
    39241 agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa catcctgaac
    39301 ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg acgactaagt
    39361 taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat actgtaggga
    39421 aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat atatttgatt
    39481 taattataga tttagatttg acttcacctt tacgtgaagt ttctaatctt gaagaattaa
    39541 tagatgaaca tctaaaaaat ttagataaag acgtctactt ttcagttgtt tctgcaagac
    39601 gtaatcccta ttttaatatg gtaaaaagaa ttgaaaatga agttactata gttaataaat
    39661 caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc tcaatgtatt
    39721 cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggcaa tgtgggataa
    39781 taaaaatgag agattatcta attttagata ttgatagtga agaagatttt aaatggttag
    39841 aatttttgtt tcctaaattt ttagaagatt cttcggagct tcaagatatc tataattatt
    39901 tatatcggag gtaaaaggat gagctgttat cttatcaccg gtggggctgg cttcattggt
    39961 tctaccttag ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga tgacttatca
    40021 atgggaaaag tagaaaatct tgatatgacg aaaaatatca cttttattga gggagatgtc
    40081 gctgagaaat ccttgatgga agagatcatg aaagctaata aatttgatta tatctttcat
    40141 ttagcagcag ttgctagtgt ggcagattca gttgcccgac cagtagaaac acatcgtgtg
    40201 aattttgaaa gtgtattaat gattttagaa ttgatccgca agtatcaacc tgatttaaaa
    40261 cggatcgtgt ttagttcttc agcggctgtt tatggggatg aaccgacttt accaaaaaaa
    40321 gaagagtccg tgattcgccc attgacgcct tatgcggttg ataaattcgc tgcagaacaa
    40381 tatgtgctag attattgtca tttatatgat gtacccggta gcgcagttcg ttttttcaat
    40441 gtctatggtc caaatcaaaa tccgaattct ccatacagtg gtgtgatttc tattctcgta
    40501 gatcgctata agaaacaaat cgtaggagaa gcaacttcat ttacattatt tggtgatgga
    40561 agccagtcta gagactttgt ttatatagat gatgtaatcc aagcattact tttagttgcg
    40621 aatgaggaag ctgcactggg gaaacaattc aatgttggaa cgggtaaagc aaccacactt
    40681 ttggaattga ttcatgcaat caatgaaatt ctcggtgtag aattgacact tgaatacaaa
    40741 gaagagcgtt cgggtgatat ccatgactct ctagcagata ttactaagat ccagtcaatt
    40801 ggctatcaac cgaaatatgg tattttaagt ggaatgacta attatttgaa aactgaaatc
    40861 aattaaaaca tactaagata agattagagg gtaaattcag gctctttgtc aataaggact
    40921 gatgagtttt gtaaaatcaa atctgggata gaa
//
LOCUS       DAFCNJ010000020        40414 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000020 DAFCNJ010000000
VERSION     DAFCNJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.020"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="LNE35_001308"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="LNE35_001308"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBK6910478.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="LNE35_001309"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="LNE35_001309"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBK6910479.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="LNE35_001310"
     CDS             complement(2303..2965)
                     /locus_tag="LNE35_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6910480.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="LNE35_001311"
     CDS             3149..4795
                     /locus_tag="LNE35_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK6910481.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="LNE35_001312"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="LNE35_001312"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK6910482.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="LNE35_001313"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="LNE35_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="LNE35_001314"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="LNE35_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="LNE35_001315"
     CDS             complement(8944..10023)
                     /locus_tag="LNE35_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6910483.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="LNE35_001316"
     CDS             complement(10126..10566)
                     /locus_tag="LNE35_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6910484.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="LNE35_001317"
     CDS             complement(10623..11033)
                     /locus_tag="LNE35_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK6910485.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="LNE35_001318"
     CDS             complement(11213..11647)
                     /locus_tag="LNE35_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6910486.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="LNE35_001319"
     CDS             complement(11747..12595)
                     /locus_tag="LNE35_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6910487.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="LNE35_001320"
     CDS             12984..14750
                     /locus_tag="LNE35_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBK6910488.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="LNE35_001321"
     CDS             14852..16111
                     /locus_tag="LNE35_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6910489.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="LNE35_001322"
     CDS             16258..17559
                     /locus_tag="LNE35_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6910490.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="LNE35_001323"
     CDS             17562..18413
                     /locus_tag="LNE35_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6910491.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="LNE35_001324"
     CDS             18426..19241
                     /locus_tag="LNE35_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBK6910492.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="LNE35_001325"
     CDS             19392..20558
                     /locus_tag="LNE35_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6910493.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="LNE35_001326"
     CDS             20788..21435
                     /locus_tag="LNE35_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK6910494.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="LNE35_001327"
     CDS             21519..22463
                     /locus_tag="LNE35_001327"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBK6910495.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="LNE35_001328"
     CDS             22598..24049
                     /locus_tag="LNE35_001328"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBK6910496.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="LNE35_001329"
     CDS             24070..24906
                     /locus_tag="LNE35_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK6910497.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="LNE35_001330"
     CDS             24906..25565
                     /locus_tag="LNE35_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6910498.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="LNE35_001331"
     CDS             25691..26380
                     /locus_tag="LNE35_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910499.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="LNE35_001332"
     CDS             26386..27342
                     /locus_tag="LNE35_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBK6910500.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="LNE35_001333"
     CDS             27386..27898
                     /locus_tag="LNE35_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6910501.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="LNE35_001334"
     CDS             complement(28154..28702)
                     /locus_tag="LNE35_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6910502.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="LNE35_001335"
     CDS             complement(28791..29972)
                     /locus_tag="LNE35_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBK6910503.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30208..31716)
                     /locus_tag="LNE35_001336"
     CDS             complement(30208..31716)
                     /locus_tag="LNE35_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBK6910504.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31875..32066
                     /locus_tag="LNE35_001337"
     CDS             31875..32066
                     /locus_tag="LNE35_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910505.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32269..33186
                     /locus_tag="LNE35_001338"
     CDS             32269..33186
                     /locus_tag="LNE35_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBK6910506.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33179..34252
                     /locus_tag="LNE35_001339"
     CDS             33179..34252
                     /locus_tag="LNE35_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910507.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34400..34918
                     /locus_tag="LNE35_001340"
     CDS             34400..34918
                     /locus_tag="LNE35_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910508.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34899..35291
                     /locus_tag="LNE35_001341"
     CDS             34899..35291
                     /locus_tag="LNE35_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBK6910509.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35367..36302
                     /locus_tag="LNE35_001342"
     CDS             35367..36302
                     /locus_tag="LNE35_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBK6910510.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36435..37214
                     /locus_tag="LNE35_001343"
     CDS             36435..37214
                     /locus_tag="LNE35_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK6910511.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37207..37524
                     /locus_tag="LNE35_001344"
     CDS             37207..37524
                     /locus_tag="LNE35_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK6910512.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37768..39378
                     /locus_tag="LNE35_001345"
     CDS             37768..39378
                     /locus_tag="LNE35_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6910513.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39535..40383
                     /locus_tag="LNE35_001346"
     CDS             39535..40383
                     /locus_tag="LNE35_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6910514.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13231 a         7260 c         7799 g        12124 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    30181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    30241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    30301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    30361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    30421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    30481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    30541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    30601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    30661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    30721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    30781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    30841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    30901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    30961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    31021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    31081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    31141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    31201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    31261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    31321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    31381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    31441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    31501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    31561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    31621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    31681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    31741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    31801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    31861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    31921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    31981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    32041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    32101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    32161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    32221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    32281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    32341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    32401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    32461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    32521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    32581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    32641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    32701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    32761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    32821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    32881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    32941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    33001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    33061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    33121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    33181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    33241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    33301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    33361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    33421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    33481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    33541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    33601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    33661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    33721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    33781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    33841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    33901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    33961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    34021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    34081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    34141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    34201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    34261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    34321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    34381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    34441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    34501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    34561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    34621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    34681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    34741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    34801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    34861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    34921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    34981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    35041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    35101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    35161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    35221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    35281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    35341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    35401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    35461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    35521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    35581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    35641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    35701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    35761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    35821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    35881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    35941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    36001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    36061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    36121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    36181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    36241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    36301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    36361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    36421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    36481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    36541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    36601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    36661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    36721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    36781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    36841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    36901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    36961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    37021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    37081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    37141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    37201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    37261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    37321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    37381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    37441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    37501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    37561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    37621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    37681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    37741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    37801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    37861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    37921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    37981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    38041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    38101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    38161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    38221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    38281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    38341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    38401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    38461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    38521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    38581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    38641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    38701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    38761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    38821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    38881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    38941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    39001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    39061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    39121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    39181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    39241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    39301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    39361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    39421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    39481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    39541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    39601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    39661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    39721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    39781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    39841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    39901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    39961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    40021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    40081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    40141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    40201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    40261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    40321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    40381 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DAFCNJ010000021        37357 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000021 DAFCNJ010000000
VERSION     DAFCNJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.022"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..470
                     /locus_tag="LNE35_001347"
                     /pseudo
     CDS             75..470
                     /locus_tag="LNE35_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(465..683)
                     /locus_tag="LNE35_001348"
     CDS             complement(465..683)
                     /locus_tag="LNE35_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910515.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="LNE35_001349"
     CDS             790..1104
                     /locus_tag="LNE35_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6910516.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="LNE35_001350"
     CDS             1208..2386
                     /locus_tag="LNE35_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6910517.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="LNE35_001351"
     CDS             2789..3082
                     /locus_tag="LNE35_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBK6910518.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            3925..6252
                     /locus_tag="LNE35_001352"
     CDS             3925..6252
                     /locus_tag="LNE35_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6910519.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            6378..6707
                     /locus_tag="LNE35_001353"
     CDS             6378..6707
                     /locus_tag="LNE35_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBK6910520.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            6747..7751
                     /locus_tag="LNE35_001354"
     CDS             6747..7751
                     /locus_tag="LNE35_001354"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK6910521.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            7748..8194
                     /locus_tag="LNE35_001355"
     CDS             7748..8194
                     /locus_tag="LNE35_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBK6910522.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            8344..9159
                     /locus_tag="LNE35_001356"
     CDS             8344..9159
                     /locus_tag="LNE35_001356"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6910523.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            9365..10537
                     /locus_tag="LNE35_001357"
     CDS             9365..10537
                     /locus_tag="LNE35_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6910524.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10699..11193)
                     /locus_tag="LNE35_001358"
     CDS             complement(10699..11193)
                     /locus_tag="LNE35_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910525.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            11776..12198
                     /locus_tag="LNE35_001359"
     CDS             11776..12198
                     /locus_tag="LNE35_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6910526.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(12364..13557)
                     /locus_tag="LNE35_001360"
     CDS             complement(12364..13557)
                     /locus_tag="LNE35_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6910527.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            13781..14158
                     /locus_tag="LNE35_001361"
     CDS             13781..14158
                     /locus_tag="LNE35_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6910528.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            14146..14484
                     /locus_tag="LNE35_001362"
                     /pseudo
     CDS             14146..14484
                     /locus_tag="LNE35_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14618..15061
                     /locus_tag="LNE35_001363"
     CDS             14618..15061
                     /locus_tag="LNE35_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBK6910529.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            15322..16137
                     /locus_tag="LNE35_001364"
     CDS             15322..16137
                     /locus_tag="LNE35_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6910530.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            16147..16809
                     /locus_tag="LNE35_001365"
     CDS             16147..16809
                     /locus_tag="LNE35_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK6910531.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            17017..18681
                     /gene="alsS"
                     /locus_tag="LNE35_001366"
     CDS             17017..18681
                     /gene="alsS"
                     /locus_tag="LNE35_001366"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBK6910532.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            18694..19404
                     /gene="budA"
                     /locus_tag="LNE35_001367"
     CDS             18694..19404
                     /gene="budA"
                     /locus_tag="LNE35_001367"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBK6910533.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            19534..20031
                     /locus_tag="LNE35_001368"
     CDS             19534..20031
                     /locus_tag="LNE35_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBK6910534.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            20216..20476
                     /locus_tag="LNE35_001369"
     CDS             20216..20476
                     /locus_tag="LNE35_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910535.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(20838..21077)
                     /locus_tag="LNE35_001370"
     CDS             complement(20838..21077)
                     /locus_tag="LNE35_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910536.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            21363..21677
                     /locus_tag="LNE35_001371"
     CDS             21363..21677
                     /locus_tag="LNE35_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910537.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="LNE35_001372"
     tmRNA           complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="LNE35_001372"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(22648..23421)
                     /locus_tag="LNE35_001373"
     CDS             complement(22648..23421)
                     /locus_tag="LNE35_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6910538.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            23565..23915
                     /locus_tag="LNE35_001374"
     CDS             23565..23915
                     /locus_tag="LNE35_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK6910539.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            23896..24612
                     /locus_tag="LNE35_001375"
     CDS             23896..24612
                     /locus_tag="LNE35_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6910540.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            24612..26060
                     /locus_tag="LNE35_001376"
     CDS             24612..26060
                     /locus_tag="LNE35_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6910541.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(26130..26639)
                     /locus_tag="LNE35_001377"
     CDS             complement(26130..26639)
                     /locus_tag="LNE35_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910542.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(26629..27354)
                     /locus_tag="LNE35_001378"
     CDS             complement(26629..27354)
                     /locus_tag="LNE35_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910543.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(27577..29697)
                     /locus_tag="LNE35_001379"
     CDS             complement(27577..29697)
                     /locus_tag="LNE35_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK6910544.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            29927..31105
                     /locus_tag="LNE35_001380"
     CDS             29927..31105
                     /locus_tag="LNE35_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBK6910545.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            31121..31879
                     /gene="aroD"
                     /locus_tag="LNE35_001381"
     CDS             31121..31879
                     /gene="aroD"
                     /locus_tag="LNE35_001381"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBK6910546.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            31902..32387
                     /gene="ybaK"
                     /locus_tag="LNE35_001382"
     CDS             31902..32387
                     /gene="ybaK"
                     /locus_tag="LNE35_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBK6910547.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(32458..33711)
                     /locus_tag="LNE35_001383"
     CDS             complement(32458..33711)
                     /locus_tag="LNE35_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6910548.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            33829..35178
                     /gene="gdhA"
                     /locus_tag="LNE35_001384"
     CDS             33829..35178
                     /gene="gdhA"
                     /locus_tag="LNE35_001384"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBK6910549.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            35290..36636
                     /locus_tag="LNE35_001385"
     CDS             35290..36636
                     /locus_tag="LNE35_001385"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBK6910550.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(36944..37330)
                     /locus_tag="LNE35_001386"
     CDS             complement(36944..37330)
                     /locus_tag="LNE35_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6910551.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT        12481 a         6450 c         7405 g        11021 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa ttaatcaagt
     3721 tttatctttt tttttcaata atcgtaaaaa aagagtattt tttatttaaa aataaaatct
     3781 ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaaa agccccaaac
     3841 tttctataca tcaataaaat aataaaaatt aaatttagaa taacgtgtaa aggattattt
     3901 gatgattttt tattgagagg agggatgaaa atgattaaga aaactcaaaa taaaatccaa
     3961 cgtatatgtc aaaaacattt tgtttctaga ctcaaagctg tatctaaaag aaaatactta
     4021 catcccatta tgatttcgat catcttgatt tttactttct tagggagctt atcaagtatt
     4081 tatgtaaacg tttatacaga aacgtcaaat tttgctaacc aaacgacaga ggagacaaat
     4141 agtccaccct attctgaaat acagtccata gaaagcacca atcagtctag caacaacgag
     4201 acaacctcaa ccacatcctc gactcaactg attgatccga cagaaaaaga aatcacagca
     4261 gcaaaagcag caggggagaa agcttattca gaaacaggta gaagccagac atttagtact
     4321 gttgcggcca ctgcgaattt caacggacat accataccag atggatatac agtgatatat
     4381 gccaaagatg gagtcattgt tgcggttaac ggatcgatga gtgcggataa aactgcgtta
     4441 caaggcgtaa ccaaagcaat cgcttttatt gataaccgtc cagatatcca taatctgttt
     4501 attggctcca cagtaaatgg tactactatc acattcccaa aaagtatcta tatgggggtg
     4561 gctaccgctg gaaatacgat cgattcagtt acacccaatg cggataaaag ttattctttt
     4621 acaccctcaa catggggagg gcttcctgcc ggaggaggta tgagtacttt ttcaatcggt
     4681 atacagttag caaatgatat tccattcacg gggtactcga tcaaaaatgg gcagatcgtt
     4741 gatggtaagg gaaatgtttc aaattttcca acctatcaag cagcaacggt tcaggtcaat
     4801 gcgaatcgga caggaacttt ttcttataca gtcaatggtg gagcagcaaa aatcggaacg
     4861 tacgggacta cattatctgg aatccatgtg ggtgacaaag tggttatcac tccaacagca
     4921 gtaacgggct attcttttgc acaaacaaac ggttctttca cagccctaga aagtaacgtc
     4981 gatacggtga cctatacagg caaacaaaca ccaatcacta ttaagtatca agactatttt
     5041 ggacagatga ttgctccttc taaaataatg aatcttactt acggaagtgc tgcacaagac
     5101 ctgacgacta atacgcctac tatcaacgga tataccttta cagctgtttc agcgatagaa
     5161 acaaagaacc aatcagcaat gagtgtttta gctagtttag atacaaatgg aaatgtaatc
     5221 gtaaaggacg caaatggaaa acagatttct gaagtcatcc tatattataa gacaaatgca
     5281 acagtatcaa tcaatgcaaa tggcagtaag tactatgatg gtctttctgt tgtaccgatc
     5341 gttaaatatg cctttaatga taaaacagaa tcaacatccc ttttaaacaa tgatctcagt
     5401 attgatcaaa tcacatggaa tccaactgat ttcaaagcga tgaatgaaaa gggagttgaa
     5461 gtaactgcgc ctactgaaat tggaacctat acgtcttggc aattgacaaa aagcggacta
     5521 gctaaactag ctgccagaac aaactattta tttactatcg tgcagacatc tgatgtctat
     5581 acgattaagc aaatcagcgg tgcggtagag ctcagtggca gtaagacata tgatggaaaa
     5641 gcaggtgtcc cgtcaatcca tgtcaagcta gcagagggtg tcactagttc gcttactcca
     5701 gtacaagtgg cacttagcag tatggattac acagcagatg cacaatcagc aaaaaatatg
     5761 gttaatgctg gcagctatac aattaaacta acgaatagcg gaatagacaa agtcaaagca
     5821 gcaaatccca agttgtcatt tactgatata gccaacacat caggaactta taccatctat
     5881 aaagctaacg cagtgattac tgtcgatgac gctgccttta attatgatgc ccaaagtcac
     5941 tcgatttcgg ttggaaatct ccacgttacc ggtgttgtct taggcgaatc attggattat
     6001 accctaacag ttaacagccg gacagatatt ggatcacaaa tagtaacgat tagtttaggc
     6061 gaaggaagtg taaacaataa ttatacgatt atcgcaacag ataccgctaa gttgacgatc
     6121 aatccgactc tcttgcttcc ttcgacaggt gggataggtc tactgccctt tgtcttttta
     6181 ggactgatct ttattttttc tggcttcttt tattttatcc atcgtaagaa aaaagcaggt
     6241 gaacagcgat gaaaaaaaca aaaaatgtac gtgtggatat atttgctgtt ctctttttta
     6301 tcctactggt cttttttata ggttctccga ctgtgtcagc tagagaatca cctattacat
     6361 ttactttgca taatcgaatg ttcgaaagcg ataagatgcc aagtatcagt caaaacgacg
     6421 gaataaatat cccggataac tttcaaggag taaaagggtt gaacggcgtt actttcgcta
     6481 tatatgatgt ctcggatgaa ttttacaagc tgcggtcaga aggaagtagt gtggaagacg
     6541 cacaaagaaa actagcacaa aaaagtgata gtgaaaaaat actggcagaa aatgtgacaa
     6601 aaacagtaga cgaggaagag ggcattgctt cttttagtgc atcggacaaa gatgagcaag
     6661 gtcgtgacgc tgtttaccga tttacggaaa taaagacttc agattaagta aaagaaaaat
     6721 cagcaccatt tgtcgttgtg cttcctgtga cagattcatc taatcataaa ttgacgacca
     6781 tccacttgta tccaaagagc gaacaaaaaa tacctgctgc actcacatta acaaaaacag
     6841 tagagaataa gcagacagat tttgcagatg gggataaagt cccttatctc atcaccgtaa
     6901 caattcctga gaacatcaac gagataggaa cctatacgat caaagataca gctgatcctc
     6961 agttatggct agaaccagaa acgatcgatt ttttgattgg tggagacaag attcatacat
     7021 ttcacacaca aaaaataaaa catggttttg tcttatcaga ttctggaaaa gatcttagtt
     7081 cgtttgctgg aaaaaaatta acgatccgtt accaaatgac attgaaagaa aacagtgaaa
     7141 aaacgacatt tgataacgat gtacgtttga cgactgacaa tcaaacagta gaaacacatc
     7201 ttgccatcca aacaggaggc aaacaatttg tcaaagtgga tcggcaaacg aaacaaaaac
     7261 tagcagatgc tcaatttcta gtcaaaaaaa atgaccaata cttagcgcaa gaaaacggga
     7321 taaatcattg ggtagccaaa gatgattcaa gagcaacgat ttttacttcc agtaaagatg
     7381 gaacattttc tgtccatgga ttgtcttttg gaagttacga actagtggag attaaaccgc
     7441 ctaaaggata tatattgagt caatcaacca ttctctttga agttgaaaaa ggcagttatg
     7501 atccgcttca tcctatacca ttggaaatca tcaacgaacc aaaagcaaat ccgacgaata
     7561 atggaaaaac gccagttcag atgcagacaa atggtccatc aaatgaaaaa aatggaggaa
     7621 gctttccaaa aacaagcgaa gagatgcttg gaggtttctc aattttaggg ttattgctag
     7681 tcctttcaac agggacagct tggttttata aaaaacaaca aaaaggaaac aataggaggg
     7741 aaataaaatg aaaaacatca aaacaaaaat atggacgttt ttaggaactg ctatcatgct
     7801 gcttcctttc gttctcggcc taggaactgc agaggtttct gcagccgtat cacctacacc
     7861 agaaaatgtt acggttaatt tgcataaatt gaaattcact tcagcaccag aaaatcaaat
     7921 aaacaacgga acggaattga catttccgaa tagcgaaccg ttgaacggcg ttgaattcaa
     7981 cgtatatgac atcactgcta cgtactatcc aagtaaagac acagcagttc cagcagacgc
     8041 tacacccttc gcaagtgtaa cgacttctgg tgaaggatta gctaacctga ctcttccagg
     8101 taaatctgat gggaaagatg ctgtatacgt ctttgtagaa acaccaaagc ccggtgtcga
     8161 aacgtcgcca aatatcgtgt taagcttacc ctagaccctg acaaaccttt agacacgatt
     8221 cacctatatg ctaaaaatgt catcaaagaa agcaatatca aaatccagaa agctgggaac
     8281 ttttcagggg tagataagtt agcaggggcg caattccaga tcaaaaattc agagaatcaa
     8341 ttcgtgaccg gttttgatga aaacactggt gtcgctctct atgatcctgc tgagtcagcg
     8401 ttgacattca caactgatac tgaaggatcc atcagtgtca atggattaat agacggcaat
     8461 tatactttag ttgaaacaaa agcaccagat ggttatcaat taaccaatcc acaaaaagaa
     8521 actactttta tagtaaaaaa cggaaaattg caacaaacaa ccggtatcat ccatggcgca
     8581 gatgaagcca atcttgggaa tccaaacggt gtagtcaatc gggaaaatac aactacggat
     8641 aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt tccacttcat caaacaagat
     8701 gtgaacacgc aagaaaaatt agccgatgca gaattttcta ttctaagagc aaaagatcca
     8761 actgttaaat taatgaaaaa aatagcaaac cttgcctcag gagaataccc atatgcttgg
     8821 tcagatgaag tcacaggttc tgactgggaa gaagtgaaat tgatttctga ttcagaaggg
     8881 aaattttcaa tcgaagggct gaaacaaggg gcatactgcc ttcaagaaac aaaagcacca
     8941 gaaggctacg cattacctaa gaatgaagct gcgtacactg aatttacagt agatgatgat
     9001 ccaacaactt ctgatgaaac aacgatcgat aaccaaccta aaggaatctt gccttcaact
     9061 ggtggtatgg ggatcatcgt attcatcttg gtaggtactg ctttagttgg aggagcagtg
     9121 atctatttca aaaaacgcca tcaagaaaca gaagcttaaa ctattggaaa caaaaatcat
     9181 acgataaata aacgatagtg attcaaatgg ggagaaaggt tgtgacaagt ggtcggtctc
     9241 ttggtattcg ctcaacaaac ggcaaaatag gaggaactat tttgccgttt gcttcgctaa
     9301 tactcaggtc aaggcgctga tttcacaacc tcttcatata catattagaa aataggtgga
     9361 catgatgaat aaaagcagaa taagaatact ggacatcttc atggcaatca ttttagtcgt
     9421 tgggatcggg atcttttctt atccttttgt agaagattca ctgaatgatt ttcttgccca
     9481 gcaaatgatc atccattatc aaaagcaagc atcgaataag aattcagagg aaataaaaaa
     9541 acagcaggaa aaaatgacaa agaaaaacca acagttggca gagcaaaacg tatcaccagg
     9601 gataggcagt ttcaaccaag cagttgatgc aaaagcatta aaagatttac ctagtaatgc
     9661 cttttttatg gaacatatgc ttggagtcat cgaaatccca aaaatcaatg tcagcttacc
     9721 tatattcgat cagacaacgg aaatattttt gcaaaaagga acgtcattat tggaaggaag
     9781 ttcttatccg actggcggga aaaacaccca tgccgttctt tctgggcatc gtggactccc
     9841 agaagcaaaa ttatttacag atttgcctaa gttgaaaaaa ggagaccagt ttttcatcca
     9901 gatcaatgga aagacattgg cgtatcaagt agaaaaaatc caagtggtct tacctgacga
     9961 agtagattct ttgggtatcc aaaaaggcag agatctcgtg actttgctta cctgcactcc
    10021 ttatatggtc aatacacatc gactgcttgt gacggggcat cgtattcctt atcaagcaaa
    10081 agaggcaaaa aaagcgatcc aaggaattga tcaatggaaa aaatggaaat tttttatttg
    10141 gtttatcggt atactacttg gatcaatcgg cttagtttgg ctactcatcg cttatttaga
    10201 ttctttggcc atcgctaagc gaaactatcc tttatctttt tatgtaaaga acacaaacgg
    10261 acgaccaatc gaaggaatgg tcttttctgt aaaaacgctc aacggcaaac actatattac
    10321 tagagaaaaa gttccatttg tgaaagcttc tgatgaatac ggactagtga gattttctga
    10381 tttaaaaggc aggaactatc gcttgcaaca tgaggagctc ctgcttaaaa tccatgtaaa
    10441 acataaacat tcgaaacagt tttcaatgaa attgaaaaaa ggaagatata aactgcgaaa
    10501 agaaaaagag gtgtactatt tgattgagaa agaatgaatg aagatgtcca aaaaagcaga
    10561 gggaacagac taaatgaaaa aagagagaag ctgacgaaat ggacgtgtaa actgctattt
    10621 ttggtttact atctggaacg ccagcttcta tttttttatg tttcagaaga attttattat
    10681 tatagtataa cttttgaatt agtttccata acatttggag caaggggtta aaccacgagc
    10741 ttgagcttcg cttaaagtgg caggggtata cgtaccattt ccacatttat gtgtatggta
    10801 ttttgaaccg gtcggtgtga catagacagt ttgttcatta ttttgctcag gagctgcggc
    10861 ttgtgcctgc tgagaagcct gttcagctgc tgcttgctgt cgcgcctgtt ctgcttgttg
    10921 ctgtgcgatt gcttcttgcc tcgcctgttc tgcttgttgc tgtgcgattg cttcttgttg
    10981 cgcttgttct gcttgttgtc gtgcaatttc ttcttgcttt ttccgttctt cttctgcttc
    11041 ttttgctttg attgtggaat caattgcagc aagacggctt gtcaactctt gtttgttttc
    11101 aggtagcgct tggatagctg ctgaagcagt tgcatagttt tcttttgtag gatttgcttc
    11161 agcttgtgac aaagcagatt ccgcttctgt cacttttttt tctagtgcag ctttttccgc
    11221 tttttgtttc tcttcttctt ttttcttttc aatttgttct ttcgatgaat ctgccgtact
    11281 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
    11341 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaacg caactaattt
    11401 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
    11461 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
    11521 tcccgtgttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
    11581 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
    11641 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
    11701 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
    11761 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
    11821 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
    11881 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaacaa
    11941 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
    12001 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
    12061 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
    12121 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
    12181 acggacgtca aagaatagtc gccgccagat agaaggaaat tagcagttga aaaagctgcc
    12241 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
    12301 gtcttattac cgcagatgca agctttttcc acacctcttt cttgtcattt acagactcaa
    12361 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
    12421 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
    12481 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataac
    12541 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
    12601 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
    12661 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
    12721 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
    12781 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
    12841 attttgcata attgaaggta gccaattcat taacccaaaa tagccggcaa tttggacagt
    12901 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
    12961 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
    13021 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
    13081 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
    13141 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
    13201 caaggacata cacgccccat attctcctcc cgctcctaca cctgcgataa agcgaaagag
    13261 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
    13321 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
    13381 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
    13441 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
    13501 ctctaaagca attcccgccg atgtcgatac taataccttt cgctgatacg ttgtcatttt
    13561 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
    13621 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
    13681 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
    13741 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
    13801 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    13861 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    13921 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    13981 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    14041 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    14101 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    14161 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    14221 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    14281 caactgaagg agttagaaga gtaactagga acagaactgt ttgtacgcga aaaccgcaaa
    14341 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    14401 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    14461 tacgatcggc tgtgtggaag ctgatagttc gaatacattg gcactattac tagaaaagtt
    14521 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    14581 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    14641 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    14701 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    14761 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    14821 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    14881 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catcattcaa agcgagtgac
    14941 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    15001 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    15061 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    15121 ttgtattttt tcatttttaa atcaaaaaaa taaagagatt tacataactt gttaaataag
    15181 cgcttacaaa aataagaaga acggaaatga atcagctata ataaccgttg ttctggattg
    15241 taatcattac aaagaaatga agattataac tttggaggtg ttcgaaatga aagaaaaaaa
    15301 gcaaaaattc gttcaaaaaa catgcccttt attcaatggt tgggtatgat tctgctgatt
    15361 gtattagctt tattgacgat cattcctttt ttatggatgg tttctgcttc attcaaagca
    15421 aataatgaag tgttcacgat tccgattcaa tggatcccta aaacttggca tttagagaat
    15481 tatctggtta tttggtctcg tattccttta gtgacatttt ttaagaatac agctttttta
    15541 agcgttgtga tcacgttgat tcagttgttt acttctagtt ttgctgctta cgggttttct
    15601 aaactaaatt ttaaagggcg cgatatttta tttttagcat atatcggcac gattgctgtc
    15661 ccttggcagt cttatatgat cccgcagttc attatgatga gaaaagtggg tttaacagat
    15721 acgttgtggt cgttggtgct attacaagcg ttcaatgctt ttggtgtgtt cttgttgaag
    15781 caatattaca gtagtatccc agattcttta tgtgagagtg cgcggatcga tggcttatca
    15841 gagtggggaa tctatcgcaa gatcatttta cctcttacaa aaccggcgat tgccagtttg
    15901 acgatcatca cattcgttaa tacatggaac gattatatgg ggccattcat ttatttgtct
    15961 tctacggaaa ataaaacaat ccagttaggg ttgaaaatgt ttattgggct atttgatgca
    16021 gagtacgcat taatcatggc tgcctcagtt gtttctgttc ttcctgttgc ttttatattt
    16081 ttagcgatgc aaaaatattt tgtagaaggg atcgcttcat caggaatgaa ggaataggag
    16141 gtgattatga aagagcagcg ttttgacaaa aatgtttata tggaattctt ttattggttg
    16201 tatcaatcat tggcaagtag cctttgtttt tggtgtgtca atatcttgta tatagcagct
    16261 gcgttccttt tgtcgataga tggaaggaat gtcttttcct ttattttttc tttacttttt
    16321 attggtccag gttgcatcgt gatgataggt atgacagaag aattagcaaa agaaaatagt
    16381 ttttcaccag tacgtttgtt cataaagatg tttcgccaat tttggctaaa gggatttttt
    16441 tattggctat ttgcttggat agttagtgtg ataatgattt ttgattgttt cttttttata
    16501 cgtttttctt atggaaaatg gttgattccg ttgtttgttt tacttgcttg tttatcagtt
    16561 tcctttagca ccaactgctg gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa
    16621 gttctacgga tagcgttcta ctatacgttg aaaaaatggt atgtgagttt actagatttt
    16681 ttactattga caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg tattctacta
    16741 ggtccttcta tcgtatttgg tttgatttac ttcaacaatc gtaaattgat gagaactatg
    16801 gatctgtaga ctttataaat tgaaactttc aattaagaat gacagttttt tttatgtaaa
    16861 aactgtcatt ctttttcatt ttttgaaaca tatggctaaa caaatgaaat aaaacgtgct
    16921 atacttttgt atgaaacagg tttagaaact gtatcactta tttgtgatta tttctaacta
    16981 ataattaaaa ataaagcata aaaaggacga tgttcaatgg ctgaaaaaat gaaacaaggt
    17041 tcgaaatctg tagtagaaag tttgattaat catcatgttg attatgtatt cggtatacca
    17101 ggtgcaaaaa tcgatggtgt atttaatgaa ttagaggatc aaggtcctga attaatcgtg
    17161 actcgtcacg aacaaaatgc tgcatttatg gcacaagcaa tcggacgtat tacaggagag
    17221 ccaggagtag ttatcgcaac aagtggtcct ggtgctagta acttagctac aggtttggta
    17281 acagcgacag cagaaggtga cccagtttta gcaattgccg gacaagtaaa acgaagcgat
    17341 ctattgaagt tgacccatca aagcatggat aatgcagcac tgttccagcc aatcacaaaa
    17401 tacagtgcag aaatccaaga tccagaaaca atttcagaag tgattgccaa tgcttatcgt
    17461 atggctaaga gttcaaaaaa aggcgcgagc tttatcagta tcccgcaaga tgtagtggat
    17521 gcacctgtcc aaggtaatgt catcaagcca ttgagcgatc ctaaattagg ttctgcttct
    17581 gcagatgata tccgttacct agctgaacgc attagagaag caaaactacc ggtactatta
    17641 gtaggtatgc gaggatcaag tgaaaaagaa acgttagcca tcaggcaatt agttgaaaag
    17701 acagcattgc ctgttgtaga gacattccaa gcagctggag tgatctcacg tgaattagaa
    17761 gcgcatttct ttggtcgtgt gggtttattt agaaaccaac caggagatat gcttttgaaa
    17821 cgaagtgatc tagttattgc aattggctat gatccaatcg aatatgaagc aagaaactgg
    17881 aatgcggaaa aagatgcgcg gatcattgtg atcgatgaag cgccagctga aatcgatcct
    17941 tttatgcagc ctgaaagaga attgatcggt gatatctcag ccactcttga tttattgaca
    18001 ggttcattag agcctcaaca agtgtcagaa gatgcaaaag aatatttagc atctcttcaa
    18061 gcaaaattaa cggagcggga cattgttcaa tcaaaaggag aagcaggtat cttgcatcct
    18121 ttagaagtta ttaacacgct tcagtcaaaa gtaacagatg atatgactgt taccgtagat
    18181 gtaggtagtc attatatttg gatggcacgc cactttagaa gttatgaacc tcgtcatttg
    18241 ttattcagta atggtatgca aacgcttggt gttgcattgc catgggcgat ttctgctgca
    18301 ttggtgcgtc caaatacaca aatcgtatca gtttccggcg atgggggatt tttattctct
    18361 gcccaggatt tagaaacggc ggtacgtaaa aaattgaata tcgttcattt gatttggaat
    18421 gatggacact acaatatggt agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt
    18481 gtagactttg gtccagttga ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc
    18541 cgagctacaa gtgtagagga attagaaaaa gctttagaag aaggatttgc gacagaagga
    18601 cccgtaatta ttgatattcc gattgattac cgtgataatg agaaattggg agaaacaatc
    18661 ttaccagatc aattctatta aggaagtgtc gacatgactg aaaaaatctt ataccagcac
    18721 gggacattag gagctttgat ggccggcttg atggatggaa cagaaacgat tgctcatata
    18781 ttagaaaaag gaactctggg attgggaaca ttgcatgggc ttgatggtga agtcatcttt
    18841 ttagatggcg tagcttatca aggaagatca gatggctcag ttgcccaatt agatggaagt
    18901 gaacttaccc cctatgcagc aattacagat tttacgccag atactagttt ttctgtctca
    18961 aaaactgcag atggagaaca attgaaaaaa gacatattag taagagaagc cggggaaaac
    19021 ctatttttgg cagtgaagat aactggatta ttcaaaaata tgcacatccg tatcatgccg
    19081 aagcaagaaa aaccttacag aagactagca aaaatttcag agtctcaacc agaattccaa
    19141 caaagcaata tccaaggaac attagtcggg ttctttacgc ctgaattatt ccaaggagtt
    19201 gctgcagcgg gatttcacct gcattttatc gatgatacac atacatttgg cggacatgta
    19261 atggattttg aagtggctga aggaaaagtt gagattagcc gcatctcgtc ccttgtacag
    19321 cattttccag ttgatgatcc gacttttgtt cacgaaacga ttgattatgc tgatttagca
    19381 gcagaaatcc aacaagcaga atagaaaaag acagcgagat gcgttcaagg tgattaacct
    19441 gacgtatctc gtttttttga ggaggaaacc tagcgtttac aaaaaaactg agctataatc
    19501 ttctccatag atcaaaaagg agcggttttt atcatgtgga cagaaaaaca gaaaaaagat
    19561 gcatacgaaa ttttattatt acagcaacaa ggattattag aagcagaaga taactggtta
    19621 gcaaatttag caaattcatc tgcattgttg aatgagactt tgcctgagac tgtctttgca
    19681 ggatattatt tattcgatgg taatgaattg attttaggac cgttccaagg gcgagtttct
    19741 tgcacacgta tcaagatggg aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca
    19801 ttgatcgttg atgatgtcaa aacccatgca aattacattt cctgcgattc agccgcacaa
    19861 tcagaaatcg tagtgccaat ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat
    19921 tgcggagtaa cgaaagggta tgatgaaatc gatcaagcat ttctagaaaa atatgtagat
    19981 cttttattag caaattttaa tataaatgct ttttcattta ttaataacta aaatcgtcta
    20041 ttgaaacaaa aaagaagaga aatcaagaat aaaaagagcc ggaaagacag tggtcatttc
    20101 tgtttttcgg tttttttatt tttttataaa atcacaagtg ttattgattt tcacaatcat
    20161 attttaatgg tattatcatt ttgaataatg aaaaaacatt aaggatggat gaaatatgaa
    20221 taacgaaact atcattttag gaaaaaaata tatttgccaa cctattggct tgaaacaaaa
    20281 agtggttgga gaagtaatca gtaaattaga aaattgtgtt gtgatctgcg tagacgaata
    20341 tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga aaagtagtgg caaaatatac
    20401 tcacgtttat gagttggaaa caacagaata tctatataac tatagcaaca aatatgaaca
    20461 tactttagtt tcataaagat ttaaataaag tgccttcaaa agcgagaaga ccaagctttt
    20521 gaaggcactt tatttagttg gcagtgattg ttcatgattt ttgatttatt caaaaggttc
    20581 gcctagagaa tctgtctctg taacgaggcc tggtggagaa actcgataaa aacctatact
    20641 gccagaactg tcctattttc ggatagacaa actagtagtt ttaaaaagat catccgatcc
    20701 aatcatttca aaaaatgcaa aactgatatc aggatcattt agctcaggct tgttatcgta
    20761 aagcatttgt aaaggttgca tttagttagg agtaaacgcg aaactatcat cattatttgc
    20821 tgaagaagat gaagaagcta attcagagtt tgtttgaaat aaaggagacg tgctgacagt
    20881 ttctttaccc tgttttttgc agaggtagaa acttttatat cggaagtttc taccggagaa
    20941 tttttctcat ttgtagaaat tgttgaatcg atactattcg aaatttgcgt atctgaaggt
    21001 atgttctttg atgcagaagt cccacaagca gtaagtaaca ataaatttga taaactgata
    21061 aacagaattc ttttcattat gtacacactc cttatagtat cgtttagcat acacctatgc
    21121 tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt tatagtatct attagtctta
    21181 gtaatacata tatggagagg gtgatatccc agttcaaacc agatacattg gttaccatct
    21241 gtattaacga ttatgcctct aaacgctttt tttatgaaaa aatttataag tgaaaacgta
    21301 ggtatgatat tatctagagg taattttttt gggtatttaa atagttgcta ggaggatagc
    21361 ttttggattt ttacagtaat tttatcttaa taatagcaat attattactt ctaaatattt
    21421 ggttttttga taagagtaga aatgcaggga taggttttag aaccaaacga agtacgtctt
    21481 ctgaaaaaaa gtgggtatat tcacagacta ttttttacgg aggggttatt tcaatcagtt
    21541 tactttcttc aacactttat tcttttaatg ttatagacgt ttccatgtca aattttatat
    21601 ctattattgg aatccttatt tctgcaatta ttacacaact acttcttgtg tttgaagaga
    21661 agtcaaaaaa taattaaagc aattatgcct tttagtacag gaattattac ttcttacaaa
    21721 tgtatagcga atgtgaagcc tgctgtttta attgttctag aaaaactttt gagtgaacaa
    21781 ttattttatg ttaggatgaa ttaatttctc tagagtttat aattaaaaaa cttatttgaa
    21841 ggaacatgct taaaccataa ttgtgttttt ccttccatca gcataaaagt tcacctcaaa
    21901 aagagtgtac gaacaaaagt tctttttgta aaccttaaaa tagattttcc tgaatacgta
    21961 ttggggattt ctaaatgttt gaatgttttt ttgaatgcaa aatcaatgca aaaaattttg
    22021 tatttctcta cttttctctc cttgaccaaa ataaaaaaac gttgatttaa caacgttttt
    22081 catacatgtt tatctgtctc aaccatacat atatggagac ggcgggagtc gaacccgcgt
    22141 ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag gattcacttt
    22201 acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt tttcaactta
    22261 cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag cacatgggcg
    22321 atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag ttgttttcgt
    22381 ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg aaacgcaatt
    22441 caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag ggttacgtgt
    22501 atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa aagcgaatca
    22561 tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg attttcttaa
    22621 gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca atgcgtggac
    22681 aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta atccgtcata
    22741 ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat cattcggacc
    22801 atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca gacgtttgac
    22861 acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac cattccgata
    22921 aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag ataagatcag
    22981 caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa catctgaatg
    23041 aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga ttttctgatc
    23101 gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt tttgacattc
    23161 ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga aagaaccatt
    23221 catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt ctgcttctgt
    23281 acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca tagcttttgc
    23341 cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat caaagaaagt
    23401 gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag tttatcttaa
    23461 caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt ggttcagtca
    23521 acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt tttttcagta
    23581 ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca aaaggaaatc
    23641 tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat tggcggctgg
    23701 ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca ttgatcggct
    23761 attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt ctgctagtat
    23821 ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat gacctggaaa
    23881 ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt ttctgaaatg
    23941 atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga tggaaaagaa
    24001 gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact agacctgaat
    24061 ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc aagtactgtg
    24121 ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg cttagagatg
    24181 ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc tcgaatgaaa
    24241 gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagcagt ttcagatgat
    24301 cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga agcgtatctg
    24361 gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt tactttagca
    24421 aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg ggattatcag
    24481 tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca aaaaatcgaa
    24541 aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt cgatgattct
    24601 ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttttgcgga atggtcgcgt
    24661 tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg attgaagaaa
    24721 acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg caaacctatg
    24781 ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg ctgctgacag
    24841 agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac gaacgtgtca
    24901 tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa tggaaaaatc
    24961 tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga caagcacagg
    25021 cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga gcacttgtag
    25081 ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg aacctgttca
    25141 aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt gcagcattcc
    25201 aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat ggtaattttg
    25261 atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat gatttcaata
    25321 aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa aatcgtcgaa
    25381 gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca atcaacggac
    25441 ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac gcgattaaat
    25501 tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg gattatgaaa
    25561 aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca gaaacattga
    25621 aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca ttgacattga
    25681 aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa atcatggtca
    25741 atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt actttagaaa
    25801 aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg tctgaacaac
    25861 aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa aatacaaaat
    25921 atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg cataaaggaa
    25981 aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt ttgcctgacg
    26041 tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg atcttctata
    26101 ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg atcaatataa
    26161 ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt ttcttcatga
    26221 gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag ttcatcaaat
    26281 gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa ttgcagcttt
    26341 actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg agagatatca
    26401 agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac atccaacagg
    26461 acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat gatcgcaaaa
    26521 ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt atgctgtttt
    26581 tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc aggtttcatt
    26641 acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga atagactaag
    26701 aatgagacag catccactga gcccaataag agtttgggca aatcccttaa gtatatctgt
    26761 gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca tcggaattgt
    26821 aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag tctttaagac
    26881 aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta cgatccacaa
    26941 taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa taaaaaggaa
    27001 taatttggtt ttagaaagac cagtcaggcg ccaattgatc aattcctgct tgttttgcct
    27061 gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata aactgaatat
    27121 gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg tagatgcaga
    27181 agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt gcaaatggta
    27241 aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac aatagagtat
    27301 tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt tcatactttt
    27361 cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc tttagtgaaa
    27421 tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag aagctgccct
    27481 attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag gagcagcgac
    27541 aataattttg tcgctgctcc ttttaaattt attttgtcat tcggaagaac ttgtggtcgt
    27601 tccgtgaagt tcttggtacg tttgcgtttt ataaagatca acagttgatt gtccgttgtg
    27661 ttcactaaag acactagttg acttgtcacc aagagatttt tcagcttctt ttaatttctc
    27721 taagccattc ttgtagtcat aatcactggt atcaattggg tctaatccac tattcgtata
    27781 gaaccgaaga agatcaccat tattgatttg gtcagaaaca gctaactgtt tatcaacttt
    27841 ttctttcagt gaatcaattt cattctgtac ttcctcagta ggttcagtaa tagcttcacc
    27901 agtttcgtta ctatagatcg taccaccgta gtaagtatat tttggtgtaa caaaatcacc
    27961 atcacggaaa gaaacaagct gttcatgatc tttcgagaat agatcttgtc ctaactgaat
    28021 ataatttttt gtatctacac ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc
    28081 gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg atatggatca tataagggac
    28141 acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc caactaattc
    28201 agctagattt ttatttcttg aattcgagac accgtaatgg tcaccgtaaa ggacgatgac
    28261 tgaattatca tacaagccgc tttctttcaa ataattgaag aattcttgta cggcggtatc
    28321 taaataattg gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat tggctaacgg
    28381 gaatcctgct tcgtcattgg tgaattttga ataaggataa tggttagata cagcaatgaa
    28441 ttttgaataa aatggttgtt gcagacgttc caagtattgt acggattgtt ggaaaaatgg
    28501 tttatcatgc aacccgtatt ggaacgaatt gtcatcatta acatcataat aagaagaatc
    28561 aaagaaataa tcatatccta aattcttgta tgtctcgttt cgattccaga atgtaccagc
    28621 gttcccatgg aataccgcac tagtgtaacc ttgtgtttga cttaaaatat caggtgcagc
    28681 ttggaaagta ttttttccgc ctaattgtgt gaacaaggag ccttgattca agccaaacaa
    28741 tgaattttcc atcagcgttt ccgcatcgct tgtttttcct gcttttactt gatggaaaaa
    28801 gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc
    28861 atgctggaga ccattctcat ctgtcaattt ataatcgatc aggaattgct ggaagctttc
    28921 taaatggata taaatcacat tttttccttt agcgagcccg aacaaatcgt catttggtgc
    28981 tgcataatgt tcttttacat aattttctac ttctttcata tcattgggac ttgcttctgc
    29041 ccgcacttgg tttgtctggt aagtttgaac accgtcataa accatgaaag cattcaagcc
    29101 aagatatttg accaaataat cacgagagaa tgttcgagtc agcaattctg gacgatcagc
    29161 ttccgccata aaaagatttc ctgagaaaat cataactgct aatgtagaga tcgcaaaagc
    29221 cattcttgct cggatcgggc gagggtccaa cttgattttt tttgcaatca gcagaacagt
    29281 caagataata aaatcgatcc aatagatcac atcatatggg cgaaacagtc gaatggcact
    29341 ttctcccaga ccactcgcta ctttcccagc acctaaaatc gtgttgatcg taatgaagtc
    29401 cgtgaattct cgataataag tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat
    29461 gaccatcatc gtgatataag caactctagg gcgtctgata taaagagcaa ttccaagtaa
    29521 taacaatgta gttgcaatag ggttgataaa caagataaag tattgaatgc cactttcgat
    29581 tccgagatgg aaatcaacta aataggcaaa aatcgttttc aaccaaagta aaacgactag
    29641 taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt gtttttatac atttcaagat
    29701 aatcttcctt tctgcacttc ttaatcacag aagtgtcatg aaaccatgtt acacagctca
    29761 cttattctca tttattttac tagtatgaca atatagtgtc aattgaaacc agaagtttat
    29821 gctgtttcat taaggttttc tttaagcttc atgaaaaaat gagcagtaag acaagtccta
    29881 tgttttttgt tatacttggg aagagaaagc taaagcaggt gaaggaatga aagtaaaagt
    29941 aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa aagaagacct
    30001 tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt
    30061 tatcgcaaaa ggatatctag gaaagcagaa taagggaatc ggttgggtat tgagccagca
    30121 gaacatccca tttgatcaat ctttttttga agaatttttt gtgaagtcaa aagaaaaaag
    30181 aactttcttt ttttcagatg attctactac cgcttttcgt atattcaatg gagaaggaga
    30241 cggaatcggc ggtctgattg ttgattatta tgcaggttat gctgtttttt cttggtataa
    30301 cgaaacgtta tatgcacatc gtgaattatt gattcaagct tttcgtgaaa tctacccgga
    30361 aatcgtagga ggctatgaga aaattcgatt tgaatcagta aatctgcctg agagccagtt
    30421 catttacgga aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc
    30481 cgtttattta aatgaaggat taatgactgg gatcttttta gaccaaaaag aagtacgggg
    30541 atcattagca gaaggtttcg cttctgggaa aacagttttg aacatgttta gttataccgg
    30601 cgccttttct gttgctgccg caatgggagg ggcaatcgaa acaacaagtg tcgatctagc
    30661 aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg atgtcgatca
    30721 gcaaaaaatc attgttatgg acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt
    30781 tttatatgac atgattgtat tggatccacc aagttttgcc agaaataaga aaaaagtctt
    30841 tcgcgtagca aaagattatg gggaactggt caaggatagt ctggatattt tagcagaaga
    30901 cgggatactt attgcgtcta caaatgctgc gaatgtctcg atagaacaat tccaaacaat
    30961 gattgaagag gaattccatg ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct
    31021 gccaaaagat ttccagacga cggatacctt tttagaaggc aattatctga aagtgtttgt
    31081 ttatcaaatt aaaaataacc attagaagga gcgatgcaga atggtttggg taaagggatt
    31141 gaatatagga agcggtagac caaagatttg tgcgcctctt atcggggccg tccgagagga
    31201 agtattacgt gaagcagttc ttgcacgtga agcaggggcc gatttagtcg aatggcgagt
    31261 cgatcattat cgtgaagttt tttcttttga tgaagtagca gaaacattgg ttcttatcca
    31321 tgagatgctt gatggactgc cgttattatt tacgtttcgt accttagctg aaggcggaga
    31381 acaagacata tcgatccgtc agtatcgcga cctttatgaa catgtgatac acacaggatt
    31441 agtagatatg gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa gactgataga
    31501 ggaaatcaag tctttaaaca ttcctttaat tatcagtagt catgatttta aagaaacacc
    31561 agcagaccct gtcttgcttt accgattgaa tgttatggaa cattttggag ccgatatcgg
    31621 aaaactggca gtaactccca ataatgaacg agatgttttg cggttgatgg agttgactag
    31681 acgggcaaat gcttttgtct ccatgcctat tattacgatg tccatgggaa atcttggtaa
    31741 aatttctcgt ctggctggcg gaacgacggg ttccgttatg acatttggtt ccttaagcag
    31801 cgattctact tctgctccag gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt
    31861 attaaatgaa ccgttgtaga acaagtttga ggtgatattt tttggcaaag aaaaaaatag
    31921 taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat tccctataca gagcacgaat
    31981 atgagtggga cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc
    32041 aatctcgtat atttaaaaca ctggttgctg tgggcaatgt gacggggcct cttgttgctg
    32101 tgataccggg agaagccgaa ttgaatctaa aaaaattagc aaaagtttca gggaataaaa
    32161 aagtagaaat gctccattta aaagatttgg aagcaacgac cggatacata cgtggtggct
    32221 gctcgcctat aggtatgaaa aaactatttc ctacttattt agatcaaatt gcagaaagct
    32281 atgaacaaat cattgtctcg gctggcagaa gagggctaca gatggagtta gcacctcaag
    32341 atatctgtgc attaacgagc ggacaatttg cagatataaa acaataaatc atacgtataa
    32401 aagaaagaag gaggccggga tgttgtttgt ccagtctcct tctttctttt ttatacatta
    32461 tgctctttgc ccggcattca ttcctgctac atgccctgta gcgaaagccg ctgtgatatt
    32521 aaagccgcca gtataaccat tgacatccag taattctccc gcaaaataca gaccatcaat
    32581 gattttactc tccaatgttt taggataaat ttcctttaga gaaacccctc cgccagttac
    32641 aaatgatttc tcaataggaa aagttttgtt gattgatagc ttaaactctt tgcatagttg
    32701 aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat ccatttcaag
    32761 ccgctcaaga aagaaaacta acaatcgctc aggcaatata ccatgccaag cattgaccaa
    32821 gttctttttg gttgctttac ttttttctgt caatatatga ataagctctt gtttggtttg
    32881 tgtagggaaa caatccagag aaagtgtcac tggttctccc gttttctcta attctttatt
    32941 gacaaaactt gaacaacgca gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa
    33001 atccatcgta tgttcagtca atacacgacc ttttttattt aaaacgcgta aagtgatgtc
    33061 ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac ggttcatccg atatcaaggg
    33121 ggactctgtc gcataaagag gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc
    33181 atctccagta gaaccagtag atggataagt ccgtccacca gttgttaaga taatggcagg
    33241 agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa
    33301 tttctctact cgagtagaga atagaagcgt cacatctagt tctttcagac gattgagcaa
    33361 tgcttcgata atcgtttttg atttatttgt tacaggaaac atccgtccat gatcctcttc
    33421 ttttaaatgt acgccttgtg attcaaagaa gttcatgata tcgaaattat tccattgcgc
    33481 aaacgtacta tataaaaact ttccatttcc aggaatatgt gcaatcaaat catcaacagg
    33541 acgattgttc gtaacattgc accgaccacc acctgtcatc agtagttttt ttcctgcttt
    33601 tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca gctgcactga tcgctgccat
    33661 catcccgctt gttccagcgc cgactacgat cacatcatac agtttttcca ttccctcacc
    33721 tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa aaaaatgttt tgagccaaaa
    33781 gagaaaatga gctaaaatga gggaagataa gcaaaggagc gtggaacgat gacaaacgca
    33841 acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa
    33901 tttttacaag caatcgatga attcatgccc acggttatgt cttttttgga cactcatccc
    33961 gaatatatcg aaaagaatat cttagaactt ttaacggaac cagaaagagt tattcaattt
    34021 cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa atagagggta ccgtatccaa
    34081 tacaactcag ctatcggccc ttataaaggt ggattgcgct tccatcctag tgtcaatcta
    34141 agtatcttga aatttttggc ttttgaacag attttcaaga atagtttaac cggtctgcca
    34201 atcggtggag gaaaaggcgg aagtgacttc gatcctaaag gaaaatctga taatgagatc
    34261 atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac atatcggccc atcgattgat
    34321 gttcctgcgg gagatatcgg tgtaggagct agagaaatcg gctatttgtt cggcgaatat
    34381 aaacgattga aaaaatatga tactggtgtg ttgacaggta aacctttgga tttttgggga
    34441 agtaagatac gtacggaagc tactggttat ggattagtgt attatgtcaa acatctattg
    34501 aacgaagaaa aagattcatt taatcaaaaa acagtttttg tttccggaag cgggaatgta
    34561 gccatttatg caatcgaaaa agtgcaagag ctaggcggga aagttgttac atgttcagat
    34621 tctagcgggt acatctatga tccagaagga atagatgtga aactattgaa agaagtcaaa
    34681 gaaatcaata gacaacgttt gacggaatac gcaaaccaac gtccttctgc tatttatcat
    34741 gctggagaat ctgtttggac attgaaagaa aaagcggata tcgctttgcc atgtgctact
    34801 caaaatgaaa tagacgaaag cttggccgaa atcttagtag aaaatgggat caaaatcgta
    34861 gcagaaggcg ctaatatgcc atgtacagta aaagctgttt ctgttcttca tgaagcaggc
    34921 gtgtggtatt gtccaggaaa agctgctaat gctggaggag tagcagtatc tgctttagaa
    34981 atgagtcaga acgtccagcg attgacttgg gaaagagagc aagtggatga gcagctggat
    35041 caaatcatgg aaacgattta ccatacgtgt cgccaaacag cagccgagta cggcgacgaa
    35101 aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa aagttgccaa agcaatggca
    35161 gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat attgtcagca ttttgtttat
    35221 ttgcttgaaa aataataaaa aaaaacgtaa agtattacct gtagagtaaa cctataagga
    35281 ggagaacaaa tggcacacat tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa
    35341 caagagttag ggtacatgca aagccaagta acagcagccc acaacgaatt acgggaaggt
    35401 actggcgcag gaagcgactt ccgcggctgg atcgatttac caacgaacta tgacaaagaa
    35461 gaatttgcac gtatcaaaga agcagctaaa aaaatccaat ctgattctga agtattagtt
    35521 gttatcggta tcggtggttc ttatttaggc gctcgtgcag cgatcgactt cttgaatcac
    35581 acattcttca atttattaga tagcaacaca agaaaagcac cacaaatctt ctttgccgga
    35641 aattcaatca gctcaacata tattgctgac ttgatcgaag taatcggtga tcgcgacttt
    35701 tcagtaaacg ttatttcaaa atctggaaca actactgaac cagcgattgc gttccgtgta
    35761 ttcaaagagc ttcttgttaa gaaatatgga caagaagaag caaacaaacg tatctacgct
    35821 acaacagata aagcaaaagg tgcagttaaa gtagaagcag atgcagaagg ttgggaaact
    35881 ttcgtgattc ctgatgacat cggcggacgt ttctctgtat taactgctgt tggtttgtta
    35941 ccaatcgctg ttagtggtgc agatatcgat gcattgatgc aaggtgccgc tgatgcaagc
    36001 aaagcatact caagcgacaa actagaagaa aacgaagctt accaatacgc agcaatgcgc
    36061 aacattcttt accgtaaagg caaagtaact gagttgttga tcaactatga accaggaatg
    36121 caatacttct cagaatggtg gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa
    36181 ggcatctatc cttcaagcgc aaacttctca actgatttgc actctttagg acaattcatc
    36241 caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca
    36301 atcacaattc ctgaacaagc agaagattta gacggtctag gctacttgca aggaaaagaa
    36361 gtggacttcg taaatacaaa agcatttgaa ggaacattgc ttgctcatac agacggtgac
    36421 gtgcctaact tgctagtcaa aattccagaa atggatgctt atacattggg ttatacaatg
    36481 tacttctttg aaattgctgt aggtatttca ggttacttga atggaatcaa cccattcgac
    36541 caaccaggtg tagaagctta caagaaaaac atgttcgcac ttctaggcaa accaggcttt
    36601 gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata agggtttttc gctagattta
    36661 aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact
    36721 caaacatact gcatcgttta ttcagaataa ggggctgtga caagacgttt gtcacagtcc
    36781 cttgtttgta tagaaatcga ttgattgcct tgctctttag tttgttttag gactttaaga
    36841 acagtgaggc tgacttcgaa ggttaattct gtttttttgt tttctagaat cagtaagcaa
    36901 aaaagcactc acttcgagtg cttttttgcg atatattctt agcttaagat aggcctaatt
    36961 tttcttttac agatgagggt accttatcgc tggtaattac ttttttctct atgatccgct
    37021 tttcgcttgc tttcaactgc aagatcgttc cttcggggta agcagaacca ttggcagagt
    37081 caccagaagt aatgacatcg aaatctaact gtcttttttc accgttttca gcataagcat
    37141 ctacgttgta tttaaaacct cttccaatta cttccccatt atctgctttg atatcagttt
    37201 ctgcaggcat tggagctttg actaccgcat aataatcttt acctacataa gtagactgat
    37261 agtagtccca acctttccat cctataaaag caatcaatat gataacgatc aaccctaaaa
    37321 tttttttcat gaaagatctc cttttttatt tttatta
//
LOCUS       DAFCNJ010000022        37077 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000022 DAFCNJ010000000
VERSION     DAFCNJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.023"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..671
                     /locus_tag="LNE35_001387"
     CDS             <1..671
                     /locus_tag="LNE35_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6910552.1"
                     /translation="LQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQ
                     APTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQ
                     DANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVA
                     IFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRK
                     KRNI"
     gene            complement(1095..1265)
                     /locus_tag="LNE35_001388"
     CDS             complement(1095..1265)
                     /locus_tag="LNE35_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6910553.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1459..1638)
                     /locus_tag="LNE35_001389"
     CDS             complement(1459..1638)
                     /locus_tag="LNE35_001389"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910554.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2025..3137
                     /locus_tag="LNE35_001390"
     CDS             2025..3137
                     /locus_tag="LNE35_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6910555.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3215..3508)
                     /locus_tag="LNE35_001391"
     CDS             complement(3215..3508)
                     /locus_tag="LNE35_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBK6910556.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3676..3990
                     /locus_tag="LNE35_001392"
     CDS             3676..3990
                     /locus_tag="LNE35_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBK6910557.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4011..5990
                     /locus_tag="LNE35_001393"
     CDS             4011..5990
                     /locus_tag="LNE35_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6910558.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6003..6329
                     /locus_tag="LNE35_001394"
     CDS             6003..6329
                     /locus_tag="LNE35_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBK6910559.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6345..6863
                     /locus_tag="LNE35_001395"
     CDS             6345..6863
                     /locus_tag="LNE35_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910560.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6863..8215
                     /gene="celB"
                     /locus_tag="LNE35_001396"
     CDS             6863..8215
                     /gene="celB"
                     /locus_tag="LNE35_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK6910561.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8299..9804)
                     /locus_tag="LNE35_001397"
     CDS             complement(8299..9804)
                     /locus_tag="LNE35_001397"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBK6910562.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9896..10486)
                     /locus_tag="LNE35_001398"
     CDS             complement(9896..10486)
                     /locus_tag="LNE35_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6910563.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10665..12311
                     /locus_tag="LNE35_001399"
     CDS             10665..12311
                     /locus_tag="LNE35_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6910564.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12360..13079
                     /locus_tag="LNE35_001400"
     CDS             12360..13079
                     /locus_tag="LNE35_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK6910565.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13558..14505
                     /locus_tag="LNE35_001401"
     CDS             13558..14505
                     /locus_tag="LNE35_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6910566.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14517..15437
                     /locus_tag="LNE35_001402"
     CDS             14517..15437
                     /locus_tag="LNE35_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6910567.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15478..16935
                     /locus_tag="LNE35_001403"
     CDS             15478..16935
                     /locus_tag="LNE35_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6910568.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17280..17894
                     /locus_tag="LNE35_001404"
     CDS             17280..17894
                     /locus_tag="LNE35_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK6910569.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17887..19611
                     /locus_tag="LNE35_001405"
     CDS             17887..19611
                     /locus_tag="LNE35_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6910570.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19608..21059
                     /locus_tag="LNE35_001406"
     CDS             19608..21059
                     /locus_tag="LNE35_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6910571.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21532..23685)
                     /locus_tag="LNE35_001407"
     CDS             complement(21532..23685)
                     /locus_tag="LNE35_001407"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBK6910572.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23687..24733)
                     /locus_tag="LNE35_001408"
     CDS             complement(23687..24733)
                     /locus_tag="LNE35_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6910573.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24883..26172
                     /locus_tag="LNE35_001409"
     CDS             24883..26172
                     /locus_tag="LNE35_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBK6910574.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26185..28887
                     /locus_tag="LNE35_001410"
     CDS             26185..28887
                     /locus_tag="LNE35_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBK6910575.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            28872..29729
                     /locus_tag="LNE35_001411"
     CDS             28872..29729
                     /locus_tag="LNE35_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6910576.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29800..30699)
                     /locus_tag="LNE35_001412"
     CDS             complement(29800..30699)
                     /locus_tag="LNE35_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK6910577.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            30868..33039
                     /locus_tag="LNE35_001413"
     CDS             30868..33039
                     /locus_tag="LNE35_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK6910578.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33107..33448
                     /locus_tag="LNE35_001414"
     CDS             33107..33448
                     /locus_tag="LNE35_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK6910579.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33724..35478)
                     /locus_tag="LNE35_001415"
     CDS             complement(33724..35478)
                     /locus_tag="LNE35_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910580.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35489..36325)
                     /locus_tag="LNE35_001416"
     CDS             complement(35489..36325)
                     /locus_tag="LNE35_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK6910581.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36327..37010)
                     /locus_tag="LNE35_001417"
     CDS             complement(36327..37010)
                     /locus_tag="LNE35_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBK6910582.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        11959 a         6519 c         7589 g        11010 t
ORIGIN      
        1 tcctgcaagg agctgttttt gagttacaag atgccaatgg aaccgttctg cagtcaggtc
       61 taaccacagg agcagatgga aaactggcga ttgacggctt agcaccagga gtgtatcagc
      121 tggtagaaac ccaagcgcca acggggtatg aattggatgc gacaccaatc gagtttgaga
      181 tcgaaagaag tcagacagcg gtggtagaat tgacgaaaga aaatcgactt acaccaggcg
      241 gtgtcgtgtt gacaaagatc gatgaccaaa gcggagaagt cctgcaagga gctgtttttg
      301 agttacaaga tgccaatgga accgttctgc agtcaggtct aaccacagga gcagatggaa
      361 aactggcgat agatggctta gcaccaggag catatcagct ggtagaaact caagcgccaa
      421 cggggtatga attggatgcg acaccagtaa ctttcaagat cgaaaaagaa caagaagtgg
      481 cgatcttttt aacaaaagag aatagaaaag ttgctgatag ctcagatata agaggaaaca
      541 atccaacaag cagtggaaat aagcatcttc caaaaactgg agaaaccatc attagccagt
      601 ttatattgtc aatattgggt gtgctacttg ttttcatcag tataaaattt agaaagaaac
      661 gaaatatatg agctaaaata aaaaattgaa taagcatagt ttttaaaatc aactagtacc
      721 catcaattga atggaaacta ccgattttag aaggggtata caaacaaata ttagtatcgg
      781 agtagagaag gttgaaccca accagtaaat aagtaaagac aattttgtgc tgcgttgaga
      841 agtgaaaaat aaacctttgt agtaatacta gttaataaaa aatatacaaa aacactgcga
      901 aaatcattct tgagtttcgc agtgtttttt gcctaatgac tcaagtttat tgggtaatac
      961 tttgattttc tttctacaaa taatcagcga tcagttgtaa ttttttctct agttttaatg
     1021 gctctttgtc aataaggaga aaaacaagtc gggtcttttg tctcggaaag cttcaaccaa
     1081 ctcgtgaata aaggttacgc ctccttgaaa ggcgtggaaa aaccaagtaa tcgatcaatc
     1141 accaatttcg catttggtag aacatgtttg gtgagctgaa agtaggtggc gttcatgtct
     1201 gtaactaaga attctatttc tttaggatta gtgcagctaa ggaaatagtt tgttaatcga
     1261 ggaatttttt gtgtaggtaa attttctcga ttaatttttc tgtctcgcca tccgcggaaa
     1321 taaaactcag tttatcctca acggaaacgt gagaacgaaa ttcacaaccc tcaacactca
     1381 agacagaatt cttttggaag actttggtaa atagcttctc aactctttca atgtgcgaat
     1441 aacggttgtc aaagatacct aacaactttt cgaaataaaa aataacgaca ctttttcagt
     1501 cagtaaagaa gtgattttat gtctgacatg atttgaaatc gagtgccttg gacggataaa
     1561 ataactttga gcgttccaat ggcttcgaca gtttttacag gtatagcgct gcttttttag
     1621 gcgtataacc attggcatat gattatatta ttcaaagtgg acaatggttt ccttcttctt
     1681 gtttttcacc accattaatt tcctatttcc atctaccaca gcagaaccac aatttctaca
     1741 gacacatgga gcagggaaga gaactgcagc aacgatcaat gtttttttct tctgtaaggt
     1801 ttcgtaagag acctctgtaa tcatcaaatt tttgtctatt gatctcagaa tttttttgat
     1861 agaaccattc ataaagcata tcgttctctc agttgtttat tttgtggtga tttaatcata
     1921 caagaaaacg atatgttttt ttataaaaac atgaatagga aaaatagttg acaagaaaat
     1981 gacaacgcca tatgatgttc catagtattt acaacctgtc atagatgact gtaaaaaata
     2041 aaagtaaaaa gaaaggacga caacaggtgg atttttatca gtctctacaa ctagatccgt
     2101 ttattttaaa acaaaaaata cgagaagcag cttctaaaaa agaaaagtgc atgtatattt
     2161 gttcattgtt tttacgatca ttattcatcg tattgtttgc catttgtttt atcattataa
     2221 ttacaacatt atttgaatcg aagcataaac catacgctgt ggttttattc tgtatgttga
     2281 tgagtattcg ttttgttgac tttggttata agatttccca ttcgatcata ggtttagcaa
     2341 tcgtcatgtt ctcactatta gtagcaccgt atgtccagct gatcaaatgg tcagttatgg
     2401 gcatgttgat tcattttatt ttactaagca gcatattaat ggcaaccgct agtgatccca
     2461 aaatggggaa tgcttctttg tacggctttt catatctctt tattgtttat tcgttaccta
     2521 aagattcgtt gaacaagaat ttctttacac aaacaagtag cttattattt ttgttttttt
     2581 gttggttttc agttctcctt tacagaaaac atcgagaaaa gaacaaagac aaaagcttgt
     2641 ttaaagagat ttttttgaaa ggcatgtatt cgcaggaaaa aatttggatg ctcatctatg
     2701 catttggcat tagtcttttg atcgttgcag gagagtatgt cccttttcag cgattgatgt
     2761 gggcagggtt tgcattttct tctattgtat ctagctatgg attgatgtcg attggtttca
     2821 aagaacgagc agtagaccga atcattggct cattgattgg atgtgtttta tttattggta
     2881 tcagccaatt tattcctttt aactgggtcg gtatattagg tggtctagct ttaggtgttt
     2941 gttctaccta tagatataaa acagttttta attcttttgg tgctttggca atcactgcca
     3001 gtttattcgg ggttccagga gcagtaacga tccgcatttt tgagaatatc cttggggtat
     3061 gtttaggaat catgtatatt ggggtgactg aaatacttat ccgaaaaata agagaaaaac
     3121 acggtttgaa tcattagatg agatcgaata aaaaataagg aaaaagtgaa gatacacatg
     3181 gatgataaat tgctatcatt ccatgtgtat ctttttacgg taaatcagtg aaacgttcga
     3241 ctttcatttt taatttatac tttttacgga gttcgtttat ttgagccatc atttcggatt
     3301 tgtgatgctt ttcgattgct tcttcatctt tccaacggtc gatcagtaaa agagattctg
     3361 gatcttctag tggaaagtaa tagtcatatt tctcgtttcc ttcttctgca cgaatcgctt
     3421 tgacaatgtt tctttcgacc atttcttctg caaattttct ggcttgtcca tttgtacttg
     3481 tatacaaaat attcactgtg atactcacta aatcccctcc tgaaaaaatt ctatcactag
     3541 tatatcacgg tgccagaaat tattattcaa gatatgatac acattttttc cgcaatgcta
     3601 cggaaatata tattttgaat agaaaacgct tacaagatat gcttggttta taaaaataaa
     3661 aggagtgatt gatttatgaa aaaagcattg attatttgtg cggcaggaat gtcatcctcg
     3721 atgatggcct caaagacaac ggatttcttt aagcaaaaaa gagaagaggt actcgtagat
     3781 gcagtttcag ctacagaagg cgataatatg attaagaata gtgattttga tttatttctg
     3841 atcagcccgc aaacgacgat gtttttggat aaattcaaaa aattaggaga atctgttgga
     3901 aagcctgttg ttagtatccc tttccaagcg tatgtcccaa tccctacagg catccagaag
     3961 ttagcagagg tgatcgaaga gaacatatga tggaatagga gtggatgata atgaaacaga
     4021 aggaaaaatt gctgttgaga tatttgattg atcacaagaa ggagtatgtc accagtcaac
     4081 gtttagcttc tgaattgtta ttatctgatc ggacaatccg taattacttg cagcggatca
     4141 aagagttagt agaaaaaaac ggaggtaaga tcattgctaa accaggttat ggctaccaac
     4201 tgcatatttt acaaagactg acatttgact tgtttttgag tcggcaagaa atagtacctg
     4261 gatatgctag agaaccgcaa ggattttatg agtccgaaga tcgacaaaag tatatattga
     4321 accaattgct tttggaagat gccattcttt ttatggatga tttggcagaa gagctttata
     4381 tcagtcgttc cagtttgaca aaggacatgc aagaaatcaa ggagcgattg attccatatt
     4441 cactaaaaat cgtttctaag catggtcaag ggacatggat cgaaggggaa gaaagaaatc
     4501 gacggcattt tatcatggat actttttttg gaaaccatta tggaaattca ttgaaagaat
     4561 atttagggca aagccgtttt ttcccagaca tcagttttga agagctagtc attatcattc
     4621 tcgatgaaac aagagaagct aagttaaaag tttcggattt tatcatacag aatctgactt
     4681 tacacttggc tttggggatc aaacggctcc gagaagggtt tgaaatcaag gaattaggca
     4741 tcgaacaaga agtttctaag cgagtagagt atcaagtggc gcagcggatc gttcaacgta
     4801 ttgaggtaat cgcaggtgta tcgtttcccg aagaagaaat tgcttatttg acattacatc
     4861 tcatggctaa gtcgaatcac aaagaaaatc aaaatgatca ggaactatca aaggaattag
     4921 cgaaagttct gaaaagattg tctcaggaat ttcatttttc attagtagag gatctccagc
     4981 ttttcaatgg actactcgat catttgtcac cgatgatgat tcgattgagc cgaggtattc
     5041 gcattgagaa cccattgact gaggagataa agaaagaaaa tcccaaagtc tttgacgcag
     5101 taaaaaggca tttcagcaac atgccggctt tgaaaaacta taccatcaat gaagatgaat
     5161 gggcctattt ggctttgcat ctcatggcgg cattggaaaa agaaagagct gcgcataaac
     5221 tacatgcatt gattatctgt gcaacgggtt acggcagtgc ccaactatta aagaatcgcg
     5281 tagtcagtga attcggtaaa aatattacag tggtaagtgt aaaaggctat tatgagatca
     5341 atgaaacaac tttaaaagga atcgacctga ttatttcttc tattgatctt tcgacaatgt
     5401 ttttcaaaat tccagtttta catgtcagta tttttttgaa tgaagatgat gttcgtaaaa
     5461 tacgaaaggt agttggagaa tctataccct atcattcctc agatagaccg ataccagcat
     5521 tttctctaca gcaaaaaaag cagatttaca ccgaacagtt gtctgaaaaa tttttcaaga
     5581 cttatacgta cgaaccgaaa aaggaagaaa ttcttcatga tttgctgcaa aagctgagca
     5641 taaatgaaga agaaggatat ataagagaaa tgcgaaagca gatccagcaa cgtgaaaata
     5701 tgggacaaat catattcagt gatacagtgg cagtcccaca tcccgctgtt ccagttggaa
     5761 tcgaaacaaa ggtagccgtt gcattgattc cttctgggat gcagtgggat caatatcaag
     5821 aaattcattt tgttttttta gtttcgcctt cttatgtcga aaatgaagga attacggtcg
     5881 tgactaaagc aatcgttagg ttagttgacc agctaaaagt acagcaggaa atcttagctg
     5941 aacctatttt tgagaatttc agcagtaaat ttatcaaact gatcgaataa ggaggaaaac
     6001 agatggctga aaaaataaac agtgaaacac tacaagtgac ttcttttgat atcattttcc
     6061 atagcggaaa tgcacggaca ctgatacatg aagcatttcg ctcgatgcgt aaagagatgt
     6121 ttgaagaagt agaagagaag ttgcgacaag caaatgaaga gattcttcat gcgcatcagt
     6181 cacaaactca acttctgaaa gaatatgcca gcgggacaaa aatagaaatg gaaattatca
     6241 tggtccatgc acaagatcat ctgatgacga cgatgacatt attggaagtt gcacaggaaa
     6301 tgaattattt gtatcaaaaa aataaataga aaggaaggat cattgtgcag gatacagtaa
     6361 aaaggaaaat cgaattagaa aaaaagcaac ttagtttgaa aaacatgtat ttcaatcgtt
     6421 atctaatcgt acgttacctg actgcctttt tctttttcat gaacatgcaa tggatgattc
     6481 tgcttctatc agctaaaagt ttaggaagta gtttaccgat gattctgatt ttagctattt
     6541 tgcctgcagt aggagaacaa gtaaagcttt atcgaaaaca ccaaacgaac gttccatgga
     6601 caaagtggta ttttttgttc cagagtgtct ttaatttatt agtgattcca gtgttgttta
     6661 cctcaggatt tacattcttg tatcctttta tggcaaataa caatggagga cagttatttg
     6721 ttttcattct tgttgtaagc ggtatttttg ccagtgcatt tatacagtat cgtttaaaga
     6781 aaatttcact caaccaagac caacagtata tccgtattaa acaatatgaa aaagcgttat
     6841 atttaggaaa ggagaataac taatgtttga ttttttgcag aaatacctga tgggacctat
     6901 ggggaaaatt gcgcaactta aaatcgttcg ggctgtcatg gcagcgggga tggcttctat
     6961 tcctttcacg attgtaggct cgatgttttt ggttttaaat attttgccat taccgttccc
     7021 atttttagaa ggatttttta atgcaacttt tttcaaagtt agtgacttgt acatgatcgt
     7081 caatacaatg acgatgggga ttttatctat ttactttgcc attgtgtttg cttatgaatt
     7141 aacgtcaatt gaacgagatg aacaaaaatt aaatgttaat ccgctcacgg gggcattact
     7201 atctgttttt gcattcttta tgtgtatccc tgaattgatc atgactgatg gtaaaatcag
     7261 cttgattgct tctatcaatg agacagaaac tgtagtaaat ggtattagga tgggctcttt
     7321 tgtcgaacgt ttaggaactt caggaatctt tacagctatt attatgtcga ttattgcggt
     7381 cgaattgtat tgtatgtgtg tcaagagaaa ttgggtcata aaaatgccag atgttgttcc
     7441 gccaggagtt tctcgttctt tcactgctct gatcccaaca tttgtcattg cgtttgtggt
     7501 gatgatcatc aatggattat tagttgcact aggaacggat atcttcaaaa tgattgctat
     7561 cccatttggg tttgttacgc agttgacgaa tacatggctt ggtgtcatgg tgatttattt
     7621 cttgatccat gcattgtgga tcgtagggat ccatggagcg aatatcatca cttctttctt
     7681 gaccccgatc gtattagcta atatggcagc taacgcacaa ggcgcaaatt atccgttggc
     7741 aggagaattc aacaattctt atgtaaccgt tggtggatca ggtgcgactt taggattgat
     7801 catctttatt gcattcctcg cgaaatctga tcagctgaaa gtgttaggaa aagcctcact
     7861 tgtaccagga ttattcaata tcaatgaacc aatcattttc gggatgccaa tcgtctacaa
     7921 tccctattta gcggttccgt ttttcttagc tccgatggct tctgctacac tcgcttattt
     7981 tgccattcgg ctggaaatcg tcaaaccgat gcttgcacag atgccgtggc cttcaccagt
     8041 cgggatcgga gcatttgttg gtagtggtgg tgattggaaa gctgctgtgt tagcagtcat
     8101 ttgtgcaatc atcgcattca ttatctggtt cccatttatc aaattctatg ataataaatt
     8161 attaaatgaa gaaagagaaa aggcagcaga attagctagt gaaaagttaa aatgatttct
     8221 aagaaaaaag gaactgtgac aaaaaatcac agtccctttt tttcttagaa atgggaaacc
     8281 actagtatat aaataccatt attcttcaat caaccgcttc acgattgcat aatccccttc
     8341 ataaggttca tctacaccat tgattttttg atagggatcc acgccttttc ccgcaataac
     8401 gactgaatct tgcggagcgc tttctgctaa agcgcgtcga atcgcttcag gacgatccat
     8461 ttcataatga acgatcaaat ctggattagt aattgcttca ttgatttctt ccgcgatttt
     8521 tttcggatct tcaaatgctg ggtcatctgc cgtcaaaaag gcgatgtcag ctgtttctga
     8581 tagcactttt ccaaaatcat gtcgacggga gattgctttg tttcctgggc tgcctaatac
     8641 aacaattaca cggccatctg gatgttcatt tttggcaaat ttcagcaatg ttttcaagct
     8701 tagataatta tgtgcatagt ccacatagac atgcgctcca ttggattgga tcaattgatc
     8761 cattcggcct ggtacacgag cttcttttaa tccgtcttgt gcatcttgac tggaggcacc
     8821 tgttagtgct gcggcaatga tcgcactggc tgcattttct tggttgaatc ctccagccag
     8881 caaaatatca taagcagtat cgcagatagc taagcgatct tcatgagaag ataaagtaaa
     8941 accgtgagta cctttatctg aacggatcac ttgataattg ctttcttccg aacgaccata
     9001 agtgatcgtt ggaatctgga atagatcggc tgtttcccta agtaaatcaa aatagtcact
     9061 ttcatgattt aaaacaactg tttttgaatt caagatcaat tgtcgcttgc aatagaaata
     9121 gtcatcaaat gtcggatgtt cgatcggact gatatggtca ggcgagatat tcaagaagat
     9181 tccgacatct aaagtcaagc cgtatacgcg ttgtgttttg taagcttgtg aagaaacttc
     9241 catcactaga tgagtcatac cattttcaac tgcttctgac atcatacgat ataaatctaa
     9301 tgactcaggc gttgtcaaat gggatttgaa atatgttttg ccatctaacg ttgtattcat
     9361 agtagaaagc aatgctgttt ttttatttgt tgtgtgatcc aagattgctt ttgtaaagta
     9421 cgcagcagtt gttttacctt ttgttccagt gaatccgatc actttcaatt tattttgcgg
     9481 atagtcgtaa aaagccatag caagaagcgc cattgcttta cggatatccg ttacgatgat
     9541 tcctactgct gaattttcgt atggttgttc tgatacataa tattgaatac cctgttcgat
     9601 tgctgaatcc aaatattctt ctttgaaatt cattccttta cagaaaaaca aagtagaagc
     9661 atcagctttt cttgaatcat aacttaactg aagaaattca gctttttctg gtacatcaag
     9721 atgccagcct cttgttgaaa caaattcttt taatagattt tcttttaata gtaaatgacg
     9781 gatgtcttct aaagagaagc tcattgattg atccctcgct ttattcaatt tcttggttta
     9841 ttatagcaca ctctttttaa tatatcgttt gtttccgcta tgacaaaatc cagctttatt
     9901 caaattttct tgacacaggg gacaagcgga tgagtatttt cataaacaat gaactgactg
     9961 taatagatag gagaacgaat agtgtctcca acaatccaaa aacataataa ttcaaaaaga
    10021 cgataagact attggtaagt aaaagtcctt gcggcattgg aagccgaaaa aatttgtgga
    10081 aaataatcgg cggtatcgtt ataccgccat ttgaggcccc gactgtgtac aatgtgtaaa
    10141 ccccaagact gaacaaaaga cttcctgaaa tcagtgaaaa gaaatgtgag gagatcattt
    10201 ccacttttgg aacaatctct aaaacaacag gaaaaagcag acttcccgct acagtcttaa
    10261 aaaatactga ttttcctaat aggacagcgg ctagaagcag catcataaga ttgatgctcc
    10321 aaacagtaac tgagcgttgg atagaaaagg ccgtttcaac taaatgtccg atggcgccca
    10381 ctccggcgga agctatatcg tgagggagca agaaccaatt caatccaact gtgataaaga
    10441 aaagacctaa aaatataacg ggtagttttt ttatttcttg tgtcattgat atctcctata
    10501 aatgttagcc ataaaaggct gtatatttct ttactttacc aaaaaaacga aaccgaattc
    10561 aaaaaaacaa ttgttcttat aaaagttata taaaatcgat tgccagtttc tgttttacca
    10621 attaaaatga tagtaatgtt ttgtagaaag ttggaatctg accgatggat aatcaaagaa
    10681 aaccagcatt agatcaatta acaccgcaag aaaaaatggc acgaggctct gcttggatga
    10741 ccgccagtaa tatcatttca agattgctag gcgctgtcta tatcattccc tggtatgctt
    10801 ggatgggcga aaatgctaag gctgccaatg gattattcaa tatgggatac aatatatatg
    10861 cattgtttct actgatctct acagcaggaa tacctgctgc gattgcaaaa caaactgcac
    10921 gctataattc attgaatgaa tatggcactt cacgacgatt atttatccgt gcattacaga
    10981 tgatgggcgg gttaggcctg ttgtttgctt tgttcatgta catcgcctct ccttggctag
    11041 ctcatgcttc tggtggcgga gaagaactga ttccaacgat gcgttctttg agtatcgctg
    11101 tattggtttt tccttgtatg agtgtgatac gagggtattt gcaaggcaat caagaaatga
    11161 tgccttatgc gctgtcgcag attgtagaac aggtggcacg tattttttat atgctattgg
    11221 ctacattcat catcatgaaa gtcatggatg gtgactatgt gacggcggtc acgcaatcaa
    11281 ctttcgcggc atttatcggt atgctagcaa gtattcttgt ccttttgtat tttttgaaga
    11341 aacatcaagc gtatacatcg gcgtttattc actacagcga aaacaaaata aatattgcaa
    11401 cgaaggaatt gttgttagat accgttaaag aagcgattcc gtttattatt gtgggttcag
    11461 gggtaacgat cttcaaatta gtcgatcagt tcacttttat gaagatcatg tctgcatcca
    11521 ccgagtattc caatgcacag ttactggatc ttttttcgat ttttagtgcc aatccagata
    11581 agttgacgat ggtagttatt gcattggcaa catcgatcgc gtctactggg ttaccgttga
    11641 ttacagaagc tgttacgttg aaagatcgag taggtctggc aaaattgaca agcggaaatt
    11701 tacagttgtt ttcatttttt atgtttccgg cgacatttgg tgttatgtta cttgcctatc
    11761 cgctaaatac attattttat acaccggatt ctctaggaag taatgtactg atccaagcaa
    11821 gttttgtagg actcttccta ggactatata tgctagtttc caacatgctg caaggaatgt
    11881 ttgaaaacaa ggcggcaatt aattatttgg ttattgggtt tctagtgaaa ctccttcttc
    11941 aatacccagc cattcgttta tttgaagtat acggcccttt attggcgaca atgatcggat
    12001 ttgctgtgtc ttgtaccttg atcttaaaga gaatccatga agtagcccgc tttaaaccaa
    12061 gttttgtctg gcgcaggaca ttgctgatct ttattcttac cttgattatg cttttggctt
    12121 cgtggatcac aaagatgatt tttggtgctt ttttaaacga agatagcaaa ttccagtctc
    12181 tattgttgat tcttgtagta gcaggtatag gggctttagt gtatggttat ttagctttga
    12241 aaatccgctt ggcagaaaga ctactaggac caggaatagc gcgtttacga cataaattaa
    12301 aaataaaata agtgaatcag aagagctaga ttgatgctct tttgaaatgg gggaaacgca
    12361 tgcgtttaga taagttttta gcagatatgg gacttggcag ccgcaaagaa gtcaaaggat
    12421 taatcaaaaa aggaacgatc gaagtcaatg gacaagtggt gaaaagcgat aaagcgcaag
    12481 taaatgaatt cgaagatgcg gttacttatt taggagaaat cattacgtat caaaaagatt
    12541 tttactacat tttgcataaa cctgcaggag tcatttctgc tactcaggat aatcatgatc
    12601 aaaccgtaat cgatttattg gaagaccagg attatcggga agatcttttt ccagtcggcc
    12661 gattagataa agacacagag ggcctcttga ttttaacgaa tgacggggtg tttgcccatc
    12721 agttactatc acctaaaaaa catgtggaga aagaatatct agctgaggtt agcggtataa
    12781 tgacagaaga agatatccgt ttatttgcag aaggactgat tattgatggc gatgagcaga
    12841 cattgccggc aaaactccaa attgatcaag tagatcaaga gacagaaact tcaattatcc
    12901 gtctgatttt gcatgaagga aagtttcacc aagttaagcg aatggtcaaa gcagtaggaa
    12961 aagaagtctt atatttaaaa agaatccgta tgggaaaatt ttggctgcct aaagatttga
    13021 ataaagggca atatcgaccc atgaccaaag aagagctaga acaagtgaga gaaaaataat
    13081 tgaatggata gctaaaagaa taagcagagt agttgtagcg ccatctgaaa agcagacttg
    13141 tccgtctgac tttcggatgg cttttttgtg aaatttcggc ttatttccgt agaagctact
    13201 tctggaacac cgtttgttcg gaataaggga ctgtgacaag acttttgtta cagtccccat
    13261 tgttccttgt gactcagagt tagatagtaa ggacacgatc tccctaatac tcaggccaaa
    13321 acgctgaatt cacaacctct gataaacggt gttcaatcag ctgaccctcg gtattagctc
    13381 aaaaaacggc aaaatatgag gaactatttt cccgtttttt tctctaatac tcaggccaaa
    13441 acgctgattt cacaacctct cctatacttt gtttcatttt ccctatactt tgtttattta
    13501 ttctcattat tgaaagcgct ataattacgt tgcgaatgaa tcgaaagggg tttttagatg
    13561 aaaaaacgaa aggggttcaa tcattttttt atgaatctat ggcgttataa agcacttgtc
    13621 ctgatggctg taccgggtat gctttggatg atttttttct tttacatacc tgtaatggcg
    13681 aatgtcgtag cattcaaaaa ttttcatatt tcagcagatg ggttcttggc tagcttgagg
    13741 gaaagtcctt gggtgggttg ggaaaatttc aaatttttat tttcttcgga tcaagcattt
    13801 ctcatcacta aaaatacaat tttatataat gtagcattta tccttttgaa tctgctgatc
    13861 tcagtcgtat ttgcaatcat tatgagcgag ttgagaaata aacgactcgt taaagtttat
    13921 caaacgatgt ccttactacc ttattttcta tcttgggtca ttatcagtta ctttgtttac
    13981 gcttttttaa gtcctgacaa aggaatattc aatcagtgga tcactggaca tggaggagaa
    14041 gctgtcaatt ggtataatga accgaaatat tggccgttta tcttggtgtt cattggtaca
    14101 tggaaaggga ttggttataa cagcattatt tactttgcat ctgttatggg aatcgatccg
    14161 acttattatg aagctgctat ggttgatgga gctagcaaat ggcagcagat caaacatgtg
    14221 acgataccac aacttattcc actaatgacg attttaacga ttttagccgt aggaaacatt
    14281 ttccgagctg attttggcct gttttataac attcctagga attccggtgc attatatgat
    14341 gtgactcaag ttttggatac gtatatctac aatggcctga catccacagg agattttggt
    14401 atgacggctg ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca
    14461 aatacgatcg cccgacgttt tgatagcgat tctgcattat tctaaggagg aaaaaaatga
    14521 aaaaaaagaa agtggagaga gtccaagtcc gctctttcaa taagacggct aattttttct
    14581 ctaatataat gattgcactc tttgctattt cctgtatttt gccatttata tttgttatcg
    14641 ccatctcttt taccgatgag tccatattgt cgactcacgg gtacagtttt tggccagaaa
    14701 agttcagtat atttggttat acgtacttgt tcgaccagat gcaggataaa attttccaag
    14761 cgcttttcgt cactgtactt gttactgttt taggtacact gatcaatagc acggctactt
    14821 ctttatatgc ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt tttaccgttt
    14881 tttgtttgat tacgatgttg ttctcaccag ggatggtggc caattatctt gttatgacaa
    14941 atcttcttca gttaaaagat acgatttggg cattgatctt gccaatggct gtcagtccct
    15001 tcaatattat tgtgatgcgc acattcttta aacgttcggt ctctgattcg attattgaat
    15061 cggcacggat cgatggagct agcgaactgc gaatatttgt tcagatcgtt ttaccgttag
    15121 ctgttccagg tattgcgaca atcagtttat ttgccgcatt gggttactgg aatgactggt
    15181 tcaatgcttt actatacatc caagatgata agctagttcc actacagtat cttttgatga
    15241 aaatccaaag taatattcaa tatctgacac aaaattcagg agcaggaagc caattgacag
    15301 gcggattagc ctcagttcca ggagaatctg ccagaatggc gatcgtagtc atctcgacgt
    15361 tgcctattgc gatcagctat ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg
    15421 gcggagtaaa agaataaaga aacaaaaata taaatcataa aattaggagg aagaaaaatg
    15481 aagttatgga agaaactagc ctttacaggt gtttcagcgt tgttgttggg aacattagca
    15541 gcatgcggag gttctggttc taaagaccag gcagaagcta gcaattcgga cactttgcag
    15601 atgtatcaaa tcggagataa accagataat ttcgatcaat tgatggaagt agcaaacaaa
    15661 cgaatcgaaa aagagatcgg tgtaaaagtc aatatcaatt acattggctg gggcgattac
    15721 gaaaagaaaa tgaacgtcat catttcttcc ggggaaaatt atgatatcgc ctttgcgaac
    15781 aactatgttt ccaatgccca aaaaggagcc tttgctgatt tgactgaatt agcgcctaag
    15841 tacgctgaaa aagcttataa tgatttagat gaagcatata ttaaaggaaa tctggtcaat
    15901 ggaaaattat atgctttccc agtcaatgga aatgtctttg ctcaacaagt acttactttt
    15961 aacaaagctt tactagataa atacgatctt tcaattgatg gaatccaatc ttacgctgat
    16021 gcagaaaaag tcttgaagga attccatgaa aaagaaccaa acacggcagc ttttgccatt
    16081 ggacaaggct tcaaagtcca agggaacttt gattatcctt tagggaatac gttaccattt
    16141 gctattgatc tggatggaga tgattcgaaa atcatcaacc aatacgataa tgaaacgtat
    16201 atcggactgt tacgtacaat gcatagctgg tacaaacaag gactcattcc aagtgacgct
    16261 gcaacgagca ataaggatta tccactagaa ggaaatactt ggctaatgcg tggagaaaca
    16321 caaggaccgt atgattatgg cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc
    16381 tcaaaagcaa ttactgttcc actaaaatct acagctcaag cacaaatggc aaattttgtc
    16441 gtatcaaata cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct gcttaacagt
    16501 gatccagaat tattgaatgg gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc
    16561 gatggttcag atgataaaat caaattgcta gatggttatc agccaaatac acatatgtct
    16621 gcttggaata caggaaacaa caagattcta tatacacaag agtctattac ggatgacatg
    16681 attgccaaac gtgatcaatc gattgctgat gcagaaactt caccgatttt aggattcagc
    16741 ttcaacactg acagtgtcaa aacagaatta agtaatattt ctaatgtcat gaatcaatat
    16801 ttagatggat tgaatacagg gactgttgat ccagatgaaa cattaccgaa attgaaagac
    16861 gctttggaca aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag
    16921 ttccgacaag aataataatg aataaagaca aaggggttgt gacaaaagac gtgtcacaac
    16981 ctcttgttcc gaataaacgg tgttcaaaag ccagtttctc ggtattaagc taaatatatg
    17041 taaaaatgtg gaaagccata ttcacaaaat ttttcttact actcgaaact aagcggctat
    17101 ttcacaaact cttggtaaac ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac
    17161 gacaaaatat gaggaactat ttttccgttt gtttctctaa taatcaggtc aaggtgctga
    17221 ttgcccaacc tttttttgta taatgagaaa gtataaactt aatgtttgga ggagaagata
    17281 tgttagggaa ggttttggaa gagttattta tccggatctg ggtcattata aaactaacct
    17341 tgtatttctg gatatataca tttgccggcg ggatcatctt tggattaggt gcggcatgga
    17401 aaacagtcaa tgaactattt tatttgtacg gattcgagta taaagaaatc acaataaaaa
    17461 gaggctggaa tatatataaa agaaattttt taagaggcaa tctgctattt agtttatttt
    17521 tatctggtac tgctttattg agttacaatt tatatttatc tgttcaaatc aaagggctca
    17581 tttttttagc tattgatttt attttacttt ttgccttgct ctgcctattt gctacttatc
    17641 aatacagtat gattttagat agtgaatata cgatctctgt tcctaatctg ctaaagctaa
    17701 gttttatttc ggttttttcc agcttttcgt cttttctaaa aataattatt ggtagcggta
    17761 taattttagg cgtgacttgg caattcaaag gattgatcct gtttggtgtc attggtcttt
    17821 tgacggtttg gaatggtacg atgacaacac attggcgtga agaattagat aaacagttgg
    17881 aaagttatga ataagtttcg gattctattt tcaaaaggaa ccttactaaa tcgtctgttg
    17941 cggaaatacg cggttatcat gatcggtgta acgatgactg ctaccgttat ttttagtatc
    18001 catacttggg aacaaaatca aaaacaggca gaaaacatga caagtgatgc cgttcaaagc
    18061 acaagcagaa tgctgaatga taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta
    18121 gggaattccg aaaagatcga aaatgtgacg acctacttaa caaaacccat agatcagtat
    18181 ctgatgtatg tatacgaaca gcaaaacagt accgatgaat tagtttcttt cccaaatcaa
    18241 atcaaagatc tctacgcaaa ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa
    18301 ctaccggagt attatgaatc ttctagagaa aacaaaggtg gggaaataaa aaaggggaca
    18361 ccaaaattac tagacgcctt ttatatcaag ttgcctatca cacaaggagg agctgagtca
    18421 ggaagtattt ttattggttt tcaaaagaaa gaactagata tgattttaga aaatctaacg
    18481 tcattcaatg gactctcttt atacatgatt tctggaacga caaatcgact atacacattc
    18541 catgataata aaatagctaa agaaacgttt gaaagacaag aaatgatcat cacagagagt
    18601 ctgaagaaaa attcacaatt gccgattgac gatctggaaa aaaacaattt tgttcaacac
    18661 caagaactgt ctgatgatta tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc
    18721 gaaacgggaa aaaatcttgc cccgctgatc attggtatca ttattttaga caatatactg
    18781 cttatctttc tttataaaac attcaaacga tattcacagc aagtatctat cgtcatgcag
    18841 gccatggatc aaacagcagc agggaattta gagacgcgga tcgacataac aaacacggaa
    18901 tatgaactga gggaactttc aatcggtatc aacgaaatgt tggatagcat caatcagttt
    18961 gtagaagaca tctacaaatt agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa
    19021 gcacagatca atccacactt tttatataat acattagaat atatccgaat gtatgcaatc
    19081 agcgaaggaa gtgaagaatt agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac
    19141 aatacgaatc aagagaaaac gatcacttta aaagaagaac tggacttttg cgaaaaatat
    19201 gtctatttgt atcaaatgcg ttatcctaat cgagtagcct atcattttat gatcgatcct
    19261 gatttggaaa agatcgaagt acctaaattt gtgatccagc cattagttga aaattatttt
    19321 aaacacggta ttgattttac tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa
    19381 ggcaaacggg taaggatcat catcaaagat aatggaaaag ggatgactga aaaacggcta
    19441 aaacagattg aagaaaaact gtctcatcct aaagtggaac ttcacggttc tatcggtctg
    19501 caaaacgtga atgaacgttt acgtgccagc ttcggcagca gttattatat gtcgcttgag
    19561 aataatgaga caggtggact gaccgtttct attacattca aggagggatg agcgtgtatc
    19621 aagtgttatt ggtagatgat gaatacatga ttttgaatgg gttaaaaaag ataattgatt
    19681 ggcaaagttt aggctttcaa atagtagcaa cagcagagaa tgccaaagaa ggactagctg
    19741 tacttgaaca gcgacagatt gatttagtag taacggatgt gacaatgcca gaaataaatg
    19801 gactggaatt tattgaagcg gctcaaaaag aacgtcataa ttttgaattt atgattttat
    19861 caggatacca agaattcgat tatttgaaag gaggtatgca attaggcgcc gtgaattatt
    19921 tgatgaaacc cgtgaataag ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt
    19981 tagatcagca aaatgaacaa aaaaatcaac aagaaatcta tcaagaactg ttattcagtc
    20041 aatggttgaa tgaagaattg gatgaaataa gtgaagaaga aataatagga caaataagtg
    20101 agaagcagca taggatagta ttgattcaac tggcaagaac tcatgatact cttatcaata
    20161 gatggttgca agaacggcaa caaatctttt actatcaacg aaattacgga gaccgtgtat
    20221 tgatcacgtt gttgttggag gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa
    20281 actttcctga tcaggaatgg ctgatcagta tcggggaaga gacagattct ttggacaaaa
    20341 tccctgaaac attccaacaa gcgaaagaca atctacagtt gcatcaattt tatggagata
    20401 aagacgagca tgttttttac gcagtacaag cagaaacaaa agatcaaacc atcgattttt
    20461 caagtttcag acgcgtgctg caaaataaac aattggaagt cgctcaaaaa atgatcactg
    20521 atttttttga acagtttcag ttagcagcca tgatgccgga agatatacga tattccagtt
    20581 ttttactgtt tatggagatt caacgggaat tgattgatct ggaagatgag gaatatttac
    20641 aaggaatcga aaaaatccat caggcaaaaa cggtgcaaga acttcagcag ctacttctgt
    20701 cattcatcaa aaagcatcag agacagaaaa agtacagtga caatgtggaa aaagtaatcg
    20761 taatcctgca ccagcattat caagaaccac ttacactcaa agaagtgtca gaaaacctgc
    20821 atttaaatgt catgtatcta ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag
    20881 cttatttgaa ccatttacgt atggaaaaag cgaaacaact gcttttgcac agtaatcaaa
    20941 acatcaatga gatagcaagt gagattggct acaacaacac cacgtatttc tctaaattat
    21001 tcaagaaaat cgtcggcaga tcccctaaag agtaccgaga aaacagtgga gaatcataaa
    21061 agaaaaagcc aatgtaatga aataacttat aaaaaattgt ctagaaatct aattttgggc
    21121 tgttcttatt acttgaggca aggcagcttt ttcatgacct ctcgataaac ggagttcaaa
    21181 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tggaaagcca ttttcacaaa
    21241 atttttctta atactcgaaa ctaaacagct ttttcacgac ctctccacta aacggtgttc
    21301 aatcagctga ccctcggtat tagctcaaaa aatggcaaaa tatgaggaac tattttgcca
    21361 tttgtctctc taatactcag gtcagaacgc tgatttcaca acctctcaat aaacggtgtt
    21421 caaaagcttg catctgcggt aataagacca accaaacgaa aaatatgagg atctattttt
    21481 gcttggctgt tcttattact tgatgcagga cagctttttc acaacctctt gttaattata
    21541 gtttgataag gaaaaaggca aatcttttgc ttgatatttc ttagggtgag ggatgatccc
    21601 taattggtaa gagatcgtgc cgcctttcag caaatcttga tgggtaaaat ataatttcgt
    21661 atattctctg tccatccgtt tgattttatg gataaattgt tgctgcggcc gattaggcgt
    21721 tgcttcaatc gtcaattcct ttccatttga caaaagcaaa acagccttgt caattaatgg
    21781 cataccgata acatattcac cggttccagg cgtgacagga taaaatccca gactattgaa
    21841 gatataccaa gcggccatac ttccattgtc ctcatctcca gggtaaccca ttggagaatc
    21901 gtcgaaggtc tgagtcatca actgtttaag cagtggctgt gccatttcag gttgtcctat
    21961 ataactgaac aagtaaggat agtgaaaact tggctggttt gaaatagcta attgtccaaa
    22021 ttctatcgcg gccatctcac tcatttcgtg gatctcaaaa ccatatcctc ctgggtcaaa
    22081 cgttgggggc tgattgcaca gctcaatgag tttttcttta tatctctcac tgccaccata
    22141 ttttccgatc agtcctgaaa aatcgtgata aacagcaaag ctgctttgcc aagcacttcc
    22201 ttctgcataa tcttgtcccc aacgattagc tgaaaatggt tctctgaact tgccatcttt
    22261 atcttttgaa cgcataaatc ctgactcata gtcaaagata ttttgatagt tcaatgattg
    22321 ctgctgatat ttcttcgtca tttcctcttc ttctagtacc ttggcaactt ggctaataca
    22381 aaaatcgcta taagcatagt ctaatgtgtg attgactgat tcgtggtagt gattaggaac
    22441 atagccatat gtcaagtaat cttctgttcc ttggcgtcca taatttggat gtttgctttg
    22501 aaccgtagca cctttttcca ttgccgttag aaattcaggc atcaagtctc gtcggatatc
    22561 ttttacagca gcatctgcaa tgaccgcatc gatcaatgtc ccaggcataa gtccacgttc
    22621 atcaggagac agccattttg gcaaatagcc actttctcta tagctgttca aaaaaccttc
    22681 cagcatttct tcgtattttt ctacagcaat caaagaataa agaggataaa ccgttttata
    22741 cgtatcccaa aacccattat ttgtatagag aacaccaggt tttatagatc gactagtcgt
    22801 atcatagtgg attttttgcc cactaggatc aatttcataa aatgtttgtg gaaataaaaa
    22861 cacccgatat aaattatgat aaaacgtccg caattgttgt tgatcatggt gtgtgagctg
    22921 tatacgatca aaataggact gccattgttc ttgactgtga gcaaggtaag tctctttgct
    22981 ttggttttgt tctagttcca ggttataagc tgcttgttcc gagctgataa aagatgtccc
    23041 aaatcggata gtttgttttt ttgtctgttc aaaatacaac gggatcgagt cgctattttt
    23101 gttggaaaca gctaactcat caattgttaa aggatattca aatctcaata taaaataaaa
    23161 agaaaaatca ggatcttctg aaccagcaaa atgtgtgatt tttccttcca ctgtgtaagg
    23221 atctataaca aagagctgat ggcgtcccgg aaaggataaa agtaaaccgc tgtcattttt
    23281 gctgtaatca atagataaga ttccgccata catgctaggt atcaatgtag aacagatgcc
    23341 ataacgtaat tgacagatcg acagatgagt cgggcaaaat atactttctt ctgggcggta
    23401 agaactttga gcatggaaaa gggtgttctc ctgtagtttt ccattgatcg gcgtaaacag
    23461 catatgactg aaatcaccca tccaaggact tggttgatgg gtaagacggt agccttgaaa
    23521 aacgcggtca tcaggatgaa accaccagct gccgttttga tccgtagttt gcggtgcaaa
    23581 ataattcatc ccaaaaggaa cgccagtata aggctggcag ttcccattag agaaatttgg
    23641 ctgattcgct gttgcatgtc tagtatctat ttgaccgata ttcattttat ttctcctttg
    23701 ttgattgtct tttgatgaat gaaactggaa atttactatc ggatggtcga tcttttgtct
    23761 ctaaccaagt gaccaaagca tctcccgcaa gcttacctaa ctgttcaaaa tcttgtgcga
    23821 tggttgtcag tggcgggtca acaagagctg ctgcttggat atcatcaaat ccgataattg
    23881 aaaaatcttc agggatatga taaccatgct gtctcaagag attcatggtc tggatagcga
    23941 caacatcgtt ttcacaaaca aaagcagtta cttcatactt agtgatataa gaaagcaatt
    24001 catctaatgt cgcgcttttg tcatccagtg aagtgtggaa agtcaaacca gctttgtcca
    24061 atgcttcaag gtaaccaagg taacgctgtc gaacggattg tggatgagtc tgcgtaccaa
    24121 acaaataagc gatttttgaa tgtccctgtt cgatcaaata agaggcagca agcgcaccgc
    24181 cttggaaatt atcggaaaga acggtaggaa aaggtaactc atagattttt ttatctaata
    24241 tgacgacagg gaattctttc aatgaaagct caaaaagtaa gtcaagatgt tgttctgtat
    24301 caagagcata ataaatcaat ccgtcaaatt cattgattag ctcttttgct gttttatttt
    24361 gtagaaagtc aagcgtcgtc atcatgacat caatctgttt atcttgcatc acagggttca
    24421 atcccttgct aaaatcacca acagacaaat cagcgacata tggaagaaga aacagaatcc
    24481 gtcgtgtttt tgtaggagaa gcaagaatct gttccggaga agcggattta acgaaactgc
    24541 ctttcccgcg aacgcgataa ataagtccta attgctctaa ttcagtcaat gcacgtttgg
    24601 aagtgattct gcttacttta taggtacgtg aaagttcttt ttcagttggt agttggctgt
    24661 ctacaggtaa ttcaccagtt aaaatagccg tttgcaaatc attaaatatt gtctggtaca
    24721 atggttgagt catataaaca cctcttaatg atatatcata ttcaaataat accataatgg
    24781 atgaaaaagt aaataaaaag atattttttg aaagcgtaga cataattggc tgtatgaagt
    24841 ataatcttta aagatatatc aaatagaaag aaggttttat tagtggttta taaagatata
    24901 ccaaaatcgg tgcagaaatt tatggaagat atcacacaaa aatgtgggga tatccatgcg
    24961 gactgggcaa agaattttac agcagcattt gccaatactc tgttgacaac agtcaaaaag
    25021 caaaatgacg gaaccacctt tttattgacg ggagacattc cagcgatgtg gttaagagac
    25081 tctactgcac aagttcgtcc ttatttagtg attgcaaaag aagatgagga attagctgac
    25141 atgatcagcg ggttagtcaa aaagcaattt ttttatatca atatcgatcc atatgcgaat
    25201 gccttcaacg aagaagctaa cggtgctggc caccaaacag atcatacaaa aatgaacgat
    25261 tggatctggg aaagaaaata tgaaattgat tctttatgct acccaataca gttggcctat
    25321 cttctttaca aaaatacagg aaaaactgat caattcaatc aatcttttat tgaaggtatc
    25381 aaaaaaatct tggacgtgtt caaaacagag caagatcatg aacactctcc ttatacattt
    25441 gtacgagaca cgacaagaaa agaagatacg ctgatcaatg acgggcgagg tgcaaaaaca
    25501 gcttttacag gaatgacatg gtctggtttc cgtcctagcg atgatgcttg ccgttatgga
    25561 tatctggtac catccaatat gtttgcggtg gtcgtgttag gttatgtgga agaaattttt
    25621 caaacgatac tcgatgataa agaaattgta cagcaaacac agaaattgaa gaaagagatc
    25681 caagagggga ttcagaatca cggacgaacg gttaatcaaa aagggcaaac gatttatgca
    25741 tatgaagtag atggaatggg aaacgccaca gtcatggatg acagtaatgt gcccaatctg
    25801 atcgccgcac cttacttggg atattgttct gaacaagagg aagattatct gcaaacaaga
    25861 agaacattgt tgagtaaaga aaatccatat ttctatgaag gaaagtacgc aaaaggaatt
    25921 ggatcctccc atacgccaga aaaatacgtt tggccgattg cattagctat ggaaggaatg
    25981 acaacgtcag acaaatctga aaaagagcgg atattgaatt tacttgtagc aactgacgca
    26041 ggaacacacc ttatgcacga aggattcgat gtgaacaatc ctgaaaatta tacgagagaa
    26101 tggttttctt gggcaaatat gatgttttgc gaattagtca tggattactt tgatattaga
    26161 gtagaaaagt aggaggtatc gtaaatgaaa aagaaagtat atatcatttc tcatagccac
    26221 tgggatcgtg aatggtacat ggcttacgaa cagcatcaca tgcgactgat tgaattgata
    26281 gacgatctac tagagctatt tgaagttgat ccgtccttca acagttttca tttggatggg
    26341 caaacgatta ttttagatga ctatcttcaa gtaaggccag aaaaaagaca ggcaatccaa
    26401 caagcaatca atgaaggaaa attgagaatt ggaccatttt atatcttgca agatgatttt
    26461 cttatcagct cggaatcgaa cgtgagaaat atgctgattg gcatggaaga aagccgtaaa
    26521 tggggaaccc ctgtcatgtt aggctatttt cccgatacat ttggaaatat gggacagacg
    26581 cctcaattaa tgaaacaagc tggtatttca gcagcagcgt ttggccgtgg tgtaaaacca
    26641 attggctttg ataatcaagt tttggaagca gaaaactatt cttcccaata ctcagagatg
    26701 tggtggaaag gccctgatca aacagcaatc tttggtctgc tatttgccaa ttggtatagc
    26761 aacgggaatg agattccagt agaaaaagaa gcagctcttg ccttttggaa gcaaaaatta
    26821 gcggatgcag aacaatatgc ctctacgaac cacttattga tgatgaatgg tgttgatcat
    26881 caaccggtac aaaaagatat atcaaaagca atccatctcg caaatgaact gtttccagat
    26941 tacgagttta ttcattctaa ttttactgac tatctggaag cagttcaaaa agatgtgcca
    27001 gaagacttag gctcagtaga aggagaattg acaagtcaag aaacggatgg ctggtatact
    27061 ttagccaata cagcatctgc tcgggtttac ttaaaacaat ggaataccaa ggtccagcga
    27121 cagctagaaa atattacaga accacttgcg acgatggctt atgaagttag tggaaactat
    27181 ccccatgatc aattagatta tgcttggaag acattaatgc aaaatcatcc tcacgacagt
    27241 atttgcggtt gttcagtcga ttctgtccat cgggaaatga tacctcgttt tgaaaaagca
    27301 gatgaagttg gaaagtatct tgcacaagat tccttagaac agctgaccgc agcaatcgat
    27361 acgaccggat tcccaaaaga cagctttcca tttgtcattg taaatactgc aggaatggat
    27421 aaaactggtg aagcagaaat aaccatcgaa ttagaaagaa aaagattcgc tgaaggaata
    27481 cctgaacaac tatatcaaga gttagaaaat ttgcctaaac gtaagtatca tgtagaaaca
    27541 aaaagtgggg ccactatacc agctatttta tctgaagaaa ctgtccaatt tggttatgat
    27601 ttgccgaaag accgtttccg tgtgccatat atggcaagaa tgatcaaagt gactttacca
    27661 ttagaaaaca tgccagcatt ttcttgggaa acattcgcgc ttgtagaagg ggaagcgaaa
    27721 gcagaagaaa aggaaacaat gattcatcag agtggacgaa tcatcgaaaa cggccctcta
    27781 catttgacga ttgaaaaaaa tggtaccatt acaatggaag atcgcaaaaa caaacgaaag
    27841 ttgaataatc tgcatatttt tgaagatatc ggcgatatag gaaatgagta tatctttaaa
    27901 caacctttct gtgatcagcc aatcctatcc agtaacaaag aaaactctga agtaaaagta
    27961 ttagtagata caccggaaat agcaaaaata agtattctac aggaaatgga aatccctgtt
    28021 tctgcagacg agcgtttaga aaaagaacag cagatggttg tggaatttcg ttatcgaaaa
    28081 gcagaacgat ccaaagaaaa acgaatcctt caaataaaaa caatcatgac aatcagaaaa
    28141 gatagtaaaa aaatcgactt tgaaacgact ctcgacaatc agatgaaaga tcatcgctta
    28201 cgtgtcttgt ttcctacaaa gttacatgtg gaacatcacg aagcagatag tatttttgaa
    28261 gtggtgaagc gcccaaacca tgtgtctaaa agttgggaaa accccacgaa tccgcaacat
    28321 cagcaagcat ttgtcaatat tcatgatgaa gagtacggtg taacagtcgg aaactttggt
    28381 ttgaatgaat atgaagtaac agaagacgga caaatcgcag tgacgctact tcgcagtgtg
    28441 ggagaactag gcgattgggg atattttcca acaccagaag cacagtgtct aggtgagcat
    28501 cggttcaatt acagcattga actacatgga ccagaagaaa aattctctac ttatttgcat
    28561 gcatacgcag cacagattcc tttttccact caacaaatca aacatcatga aggaacattg
    28621 atttcaaaac aacaatattt aacgataaaa agtgaaacgt ttgcaattac agctttgaaa
    28681 cgcagcaagt ttagtgataa agtagtagta agaggtttca atatgtctag tcatttagaa
    28741 aaattggaaa taactaaaga caatggaaaa actgtcatat tgaatctgct tgaagaacca
    28801 acgaagcaag cggttgtccc aataatccaa ccttatgaga ttcggacaat cggattcgag
    28861 gaggaaaacg aatgtatggc ggaatagaag caggcggaac aaaatttgtg tgtgcggttt
    28921 cagatggtgg agaaatttta gaaaaattaa gtatccctac cacaacacca gaagaaacat
    28981 tggatcaggt atttgaattt tttgatcgtt atgaattgaa gggaatcggg attggctcat
    29041 tcggcccgat tggtatcgac aaaacaaaag ataattacgg atacgttttg gcaactccca
    29101 aaaaagggtg ggcacaattc gattttcttg gcacaatcaa ggaacgctac aatattcctg
    29161 ctgcttggac aacggatgtc aatgctgctg catatgggga attgaagcaa ggagcagcac
    29221 aaggaaaaaa tagttgtatt taccttaccg tgggaacagg aatcggtgca ggtgtcgtga
    29281 tcaatgaaga aattttttca gggatcgcac atcctgaaat gggacatatt tgggtgaaac
    29341 gccatccaga tgaccattat gaaggtactt gtccttatca taaagattgt ttagaaggat
    29401 tagcagcagg accgtctatt gaggcacgca cagggatcaa agggcaagat ctgccgcaag
    29461 atcatcctgt ttgggctatt caggcttatt atattgctca agcactgatc aactatacgt
    29521 tgacgttagc acctgaaaag attattttag gcggtggggt aatgaatcag gatcatctcc
    29581 ttcaaaaaat ccgtcagcaa tttgttgaat tgatgggtgg ttatatggaa acacctcaag
    29641 taagtgaata tatcgttcgt tggggactgc cgaatgaaag tgggattatc ggtagtttgc
    29701 tgttagcaga acacgaacat caaaagtaag cagacaaatt acacaaaaag aacagagtct
    29761 tatcattatc tgctgataag actctgttct tttttatttc tattcccata atccaggtaa
    29821 agctttaccg actatttttt cttgagtaaa tggatggatc atgtgcagtt ctaatgcatg
    29881 gagcatcagt cgttcagctg ttctattttg gtagagaggg tctccaacga tcggatgtcc
    29941 cttatgagct aaatgaactc tgatctggtg tgtacgtccg gtttccaaaa cacaatagac
    30001 ttgtgtttgt tcagaaagta ctttgttgac agtgacatgt gtcaaggcgc tctgaccatt
    30061 tttatcatct acgacccttt ttctccgatc atggcgatct cgtccaattt tattggtgat
    30121 ggtcatatct ttggcaattt ctccccatac gatggcttga tatctccgat agatcatttt
    30181 ttgttccaac attcttccaa gtatcggcaa gacaaatgga ttttttgcaa ataaaacggc
    30241 accgcttgtt tctttgtcca agcggtggac aacgtaaggt cgttgctgtt tagtgtcgag
    30301 ataagcagct aaatgattga gtaaggtact gttttctgtc ggttcatttg gatgagtctt
    30361 cattcctgca ggcttatcca ctataatcaa atgctcatct tcatataaaa cacggatgtg
    30421 cttcgcatct cctaattgaa cttcttgata attataatct gtttcttcga ataacaatgt
    30481 gatctggtca ttgcccttta cttcatgatg gaacaaagca ggttgttgat tgatccaaac
    30541 gtttttacgt atacgcaaaa agtgtcgaac ctttctagga actaaccatt ccttttcaag
    30601 caagtcgcga attgttgttt caggatggtt cataggtaaa gtgatcgtga ttttcacaag
    30661 tgtactcctt tcagtgcaat tgcagactaa ttgtaccatt tttaaaacat atttaagaaa
    30721 aaacttacat ttttctgttc attcttatgt tactctactt gcactaacgt aaaaaaacat
    30781 attgaactac aacttaagca ggaagaaaac actctttcac tatggtaaaa taagacgatt
    30841 gaatcaaata gacaggagaa gaagcaaatg gattttcaaa gcattttaga aaagataaaa
    30901 gctgctttga tcagtttttg gcgttggatc agaccatatc tcggacaatt ccatcgatgg
    30961 cgaaagcgca tctggaaaaa atatcatgtg aacaaattga ttttgctgat tggtttgatc
    31021 tgtgtcttag tgacaagtat ctacctcttt attttagcaa aacaagcaaa tgtcgaaacg
    31081 ttaaaatcag gattaagcca atcgacagtc atttatgata agaaaaacga agaagctggc
    31141 acactgtacg cccaaaaagg aacatatgta gaattaaacg cgatttctcc ctatatccaa
    31201 gatgcagtaa tctcgacaga agataggaat ttttacgagc atcacgggtt tgatatcaaa
    31261 gggattgccc gtgcagcagt ccgaatggtc ttgtctggcg gaacatcagg gggcggagga
    31321 agtacgatca ctcagcagct tgccaaaaat gcgtatctga cattagacca aacgtttgat
    31381 cgaaaagcga aggaattatt tttggcaatc gagatcgaaa agaagtacag caaagaagaa
    31441 atcttgacca tgtatcttaa caatgcctat tttggcaacg gggtatgggg tgtccaagac
    31501 gcttccagaa agtattttgg agtagatgca acgaatgtta cgctgtctga ggctgctact
    31561 ttggcaggaa tgctgaaagg tcctggtatt tataatccga tcgatcaccc aaaaaatgcg
    31621 aatgatcgga aaaacacagt actaagtgta atggaagaaa acggaaaaat cacaaaagaa
    31681 caagaacaag cagaaagcca gacagatatc agcgcgtatc ttaatgacac atacgataat
    31741 tcagattctg gttatcgtta tccttattat tttgatgcag tgatcgatga agcagttaac
    31801 gagtatggtc ttgatgaaga agatgtcatg aataaaggct ataaaatcta tacagcttta
    31861 gatcaggatt atcaaaagca attagaagct acttatcaaa acagtgcatt attcccagca
    31921 aatgcttcag atggtgccat ggtccaatct gcttcagtag ctttagatcc gaatacaggt
    31981 ggtgtacaag cactagtagg acgaagagga gatcatgtct ttagaggatt cagctttgct
    32041 acacagatgc gtcgttcacc aggatcaacg atcaaaccat taagcgtata tacaccagca
    32101 ctggaagctg gatacaagcc ttccagtatc ttgaaagacg aaccgcaaag ttattacgat
    32161 gcgcataatt ttgacgggac ataccaagga gaagtaccga tgtatcaagc tgttgcacaa
    32221 agtttgaatt tgcctgctgt atggcttttg catgagatcg gtttacaaaa aggatatgat
    32281 aaagcagaag aattcggcct gcctttagca aaatctgata agtattatgg cttagccctt
    32341 ggtggactgg aaaaaggagt atctccattg atcatggcgg gtgcttacag tgcatttgcc
    32401 aatgatggtc agatgtatac gccgcatctt atcacgaaaa tcgttgattc gactggagcg
    32461 gtgatcgtcg ataacacaaa caaaaaacca aaacaggtca tatcaaagga aacagccgat
    32521 gaaatgacca gcatgcttct tggtacattt tcaaatggaa cagcggctgc tgcgaatcct
    32581 gcaggttata cgatagcagg gaaaacagga accacagaaa ctaatttcga tgccacaaaa
    32641 gcaaatgacc aatggatgat cggttacacg ccagacgtag tgatttcgac atggatgggt
    32701 tacgaagaat ccagcaagct gcactatttg gaagggacaa gtggaaatgt tgtcgggaaa
    32761 gtcttcaaat cagcggcaga aggcatcctt ccgtatactt caaaaacagg atttagtgta
    32821 gcagatgcct atgccacagg aggacaagtg gtagctgcag atcaagttgg aaacacagga
    32881 aattctaatg gagagtctgg aaattggcaa gataacttga atcaatatgg tgaacaagca
    32941 aaagaagggc tgaaaaactt cggcgatatg gtgaaagaag gcgttcaggg aattggtgaa
    33001 gcggctaaag acctctggcg gaaatatcaa ggagagtagc ccctttgaaa acgaataacc
    33061 ccatgttaca atagagagta actactaaaa gaaaagaggg cataacatgt caaacatcta
    33121 cgatagcgcg aatcaaatcg aacgtgagat ccgtgaatta ccagaattca aagcgttgaa
    33181 agaagcgtac gagaaagtac aagcaaacga agaagcacac aaactgttca aagacttcca
    33241 agcgatgcaa atcgaattac aagaaaaaca aatgagtgga caagagttca gcgacgaaga
    33301 tgcaatgaaa gcacaagaaa tggctgtgaa aatccaatca gaagaagtaa tcaatgactt
    33361 gatgcaaaaa gagcaaggct tcagtacaat catcaatgac ttgaaccgta tcatcatgac
    33421 accagttcgc gacctttaca gcgaataaaa ctattttatg aaacaacaga gggtgtgaaa
    33481 gagacgttcg gccttgagcg atgacaaaat tgcttcttat tgccgatagg ctgtctcttg
    33541 aacaccattt accaagaggt cgtgagccta ccgtttaact ctgagttaca aggaacaatt
    33601 tcaaaaaaca gcttctcatg tttgatgact attgtgactt gtgaccgagg agttggcagg
    33661 agaacaccgt ttattcagaa ataggagcta cgacaaaagt cttgtcatag ctcctatttt
    33721 ttgtcattca tttttcaagc ggtagaaata gcctttttga gctaacagtt tttcgtaagt
    33781 acctatttca acgatttttc cttcatcgat cacaacgatt ttatctgcct ctttgatcgt
    33841 tgaaagccga tgagccacaa taaaagtagt gggtgtttct gtcaggtaat tcagggcttg
    33901 ctggattttt ttctctgact ggttatctaa agctgaggtt gcttcatcta gcaaaatgat
    33961 ttttggctga cgtatcaacg ctcgtgcaat ggagatacat tgcttttggc caccagacag
    34021 cttgttgcca tgttcgccta ctaaggtatc cagaccatca ggaagctggt caatcagtga
    34081 agaaagttgc gcagcttcga tgacttcatc taaacgttct tttgatacgt ttttcaagcc
    34141 ataagtgatg ttctctttga tcgaagcgga gaacaacaaa gtggtttgag gaacaacggc
    34201 taaataattt cgcacacttc gcatatccag ttcatccaaa ggctgatcat ctagaaaaat
    34261 ttttcctgaa gtcggctgga taaatccaat caataagtta aggagtgtag attttccaga
    34321 gccagatggc ccaacaaaag caattgtttc tccagctttc acctctagag aaaaatgatc
    34381 gataatcggt ttgagtgagt ccggataaag aaaagaaaca tcctcaaaac gaaaatttcc
    34441 atccaaatgt tggatttttt tcttaccgtg ataagtttct acatcgccag caatcaacag
    34501 ttcgccaata gaagtgatag attccatgcc ttttgaaatc gtaggaatca gcgtgatcat
    34561 tccagaaacg gaattgacga tcgtcgtgaa atacgtctga tacatcacga catcgccggg
    34621 ttggatgcgc ccttgtaaag ctaatatccc agtgaaaatc aaacaaatca gctgaaatat
    34681 ctgaaaacta gcccagctga ctgatccgaa aacagattgc aggatatcta agtggaatcc
    34741 tttttcatag atttgagtaa aatgcttatt taagcgagtt gtttcgtgtt tttctaaccc
    34801 atgagctcga gcaatcggtg ctaattcgat cacttcgatc acttttgcag aagtctcttc
    34861 catttcttgc ctgaagtctg agttggactg tctgatcctt ttacggaaaa agagagtaac
    34921 gaatgcggcg agaggaacag tcaataagaa aaacatgaaa acaacaggac ttttataaag
    34981 cgtgatacct aaagctacga ataaattgac agcgatattt aatccgctga caaatacttg
    35041 agaggacaac gtttggatag cttctacatc acggatgatc tttgactgga ttcgtccaga
    35101 ttgcaattct ttttgataag gaatagatag ttcttggatt ttcttgataa gggtcgctct
    35161 tagtccagct tctacttgac ggatcgaagt gctatggtat tttacatgaa aataattcgt
    35221 caatatattt tgaccaatca gacaagccat caaaaaagcg ttgaaataaa tggcctgcaa
    35281 ctgagaagaa tctttcgatg aagctgcatt gattacattg gcaatagcga tcggcattaa
    35341 ccaagatgga agatgtttga taataaaaaa gagtcctgac atggcaaaac gccaataatt
    35401 tccctgataa agatgcagca atgtctgaaa agcatttgca tgatattttt gataactttt
    35461 ctgtattcgt tcctccataa tcttctcctt aacaatcttg gtaaataaac tgcaagtgtt
    35521 ctattgcttc aggaacataa atctcaggag tagcttcaag agtggcgtac attagaggac
    35581 gctcatattt gatataatgc agtaccgggc ggaaatctaa acagcccttg ccaacaggta
    35641 ccatgcggat ctttccattt tccatgatat agtctttgag atgaagcgct gtaatataac
    35701 catgtaaatt gtccaaggca cctcggatca ctttttcctg atcagcatga ttttctttat
    35761 gcatcaaatt cgcacaatct aaaatgattt ttaaatttgg tgaagctact tcatcgatta
    35821 aacgtttagc taactgatag ttatagattg gatgattgat ccctgcttca acagcaacag
    35881 ttacgccaaa attttctgca tgagcgacta attcaatgat agaatttctg gcgatctgat
    35941 aagcttcttc agtaaaattt tcttttgtgt aaccgacaga gacactaccg gtttccgtag
    36001 caatcatctt ggcttgatag gcagagcaca gagcaatttg ctgtttgaaa gttgccaatg
    36061 cttgtgcacg aacttcttga tttttgctgg ataggttgat atagcatcct aaaatggaaa
    36121 gttcgatatt gtttttttgg aagtattcac caaaataggc agcactccca agtgtcatgt
    36181 cagttaattt aagttgttca ggaaatgctt tttgtggtgc aaattggata tgggacaagt
    36241 gaagctgtga tactttttga acgatttctt cacgatttcg aacatctaaa tcgtgggcac
    36301 ggatgcctaa atttaatgga aacatctcaa actccttttt gtaaataatt agcaagaggc
    36361 agacgggatt cttttatcaa ttcaacaatt attcgggcaa ctttatcagc gccttctgga
    36421 ctaaagtggg tattgtcttt tagtccttct gggtaatttt tacaagtatc aggcataaga
    36481 tgcaggtaga atttttttgt ctcttctttt gagaaggtct ggaacaattc ttgtgatctt
    36541 gagaacacat ctaaaagagg aatatggtgt ttctcagcaa atgcacgcat cgcttttgga
    36601 tagtctccaa gtatatctgg attcaatgtt ccgttttcta agtaatctcg tcgcgtcaca
    36661 gatgtcagta agaccatttg gacgttcgct ttttttgcgc attcaaaaaa tatctgcaga
    36721 ttttttaaat agctaccgaa gggaagcgta cctctttctt cttggatttt ctggtcgtta
    36781 tgtccaaatt gtacaaataa gaaatcattc ggctgaaact gtgaagctag acgggctaac
    36841 cttccttcct ggataaatga tttggtgctg cgtccattct ttgcttggtt attgatctgg
    36901 atctcgggtg aaaaataagc agagattttt tctccccaac ctgtctcggg acgcttgttt
    36961 tcatcttttt ctgccgcagt agaatcaccg gcaatatgga tagtaggcat gttatctctt
    37021 ctttcataaa ggtttatagg caatgaagca gaacagcaag agaagaagac ggagcaa
//
LOCUS       DAFCNJ010000023        34869 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000023 DAFCNJ010000000
VERSION     DAFCNJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.024"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..187)
                     /locus_tag="LNE35_001418"
     CDS             complement(59..187)
                     /locus_tag="LNE35_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK6910583.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="LNE35_001419"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="LNE35_001419"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK6910584.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="LNE35_001420"
     CDS             complement(1674..2729)
                     /locus_tag="LNE35_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK6910585.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="LNE35_001421"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="LNE35_001421"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBK6910586.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="LNE35_001422"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="LNE35_001422"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBK6910587.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="LNE35_001423"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="LNE35_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBK6910588.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="LNE35_001424"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="LNE35_001424"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBK6910589.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="LNE35_001425"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="LNE35_001425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBK6910590.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="LNE35_001426"
     CDS             10491..12050
                     /locus_tag="LNE35_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBK6910591.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="LNE35_001427"
     CDS             complement(12161..14092)
                     /locus_tag="LNE35_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6910592.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="LNE35_001428"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="LNE35_001428"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK6910593.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="LNE35_001429"
     CDS             complement(15021..15773)
                     /locus_tag="LNE35_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6910594.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="LNE35_001430"
     CDS             complement(15932..16141)
                     /locus_tag="LNE35_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910595.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="LNE35_001431"
     CDS             complement(16152..17480)
                     /locus_tag="LNE35_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910596.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="LNE35_001432"
     CDS             17616..17771
                     /locus_tag="LNE35_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910597.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="LNE35_001433"
     CDS             complement(17845..18432)
                     /locus_tag="LNE35_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBK6910598.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="LNE35_001434"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="LNE35_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBK6910599.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="LNE35_001435"
     CDS             complement(20012..21301)
                     /locus_tag="LNE35_001435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBK6910600.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="LNE35_001436"
     CDS             complement(21455..22387)
                     /locus_tag="LNE35_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910601.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="LNE35_001437"
     CDS             complement(22544..23203)
                     /locus_tag="LNE35_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK6910602.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="LNE35_001438"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="LNE35_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK6910603.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="LNE35_001439"
     CDS             complement(24244..24534)
                     /locus_tag="LNE35_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910604.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="LNE35_001440"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="LNE35_001440"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBK6910605.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="LNE35_001441"
     CDS             complement(26820..28175)
                     /locus_tag="LNE35_001441"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBK6910606.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="LNE35_001442"
     CDS             complement(28199..29368)
                     /locus_tag="LNE35_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910607.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="LNE35_001443"
     CDS             complement(29365..30252)
                     /locus_tag="LNE35_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBK6910608.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="LNE35_001444"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="LNE35_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBK6910609.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="LNE35_001445"
     CDS             34332..34853
                     /locus_tag="LNE35_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK6910610.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9400 a         7569 c         6284 g        11616 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtg
//
LOCUS       DAFCNJ010000024        34636 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000024 DAFCNJ010000000
VERSION     DAFCNJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.025"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            591..1280
                     /locus_tag="LNE35_001446"
     CDS             591..1280
                     /locus_tag="LNE35_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6910611.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="LNE35_001447"
     CDS             1267..2475
                     /locus_tag="LNE35_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6910612.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="LNE35_001448"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="LNE35_001448"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBK6910613.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LNE35_001449"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LNE35_001449"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBK6910614.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="LNE35_001450"
     CDS             complement(6578..7273)
                     /locus_tag="LNE35_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBK6910615.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LNE35_001451"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LNE35_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBK6910616.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="LNE35_001452"
     CDS             complement(8472..8663)
                     /locus_tag="LNE35_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBK6910617.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="LNE35_001453"
     CDS             complement(8684..9574)
                     /locus_tag="LNE35_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBK6910618.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="LNE35_001454"
     CDS             complement(9561..10328)
                     /locus_tag="LNE35_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6910619.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LNE35_001455"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LNE35_001455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBK6910620.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LNE35_001456"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LNE35_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBK6910621.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LNE35_001457"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LNE35_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBK6910622.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="LNE35_001458"
     CDS             14899..15282
                     /locus_tag="LNE35_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910623.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="LNE35_001459"
     CDS             complement(15450..16208)
                     /locus_tag="LNE35_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBK6910624.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="LNE35_001460"
     CDS             complement(16226..17044)
                     /locus_tag="LNE35_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK6910625.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LNE35_001461"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LNE35_001461"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBK6910626.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LNE35_001462"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LNE35_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBK6910627.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="LNE35_001463"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="LNE35_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBK6910628.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="LNE35_001464"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="LNE35_001464"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBK6910629.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="LNE35_001465"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="LNE35_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBK6910630.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="LNE35_001466"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="LNE35_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBK6910631.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="LNE35_001467"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="LNE35_001467"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBK6910632.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="LNE35_001468"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="LNE35_001468"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBK6910633.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="LNE35_001469"
     CDS             27086..27385
                     /locus_tag="LNE35_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBK6910634.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="LNE35_001470"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="LNE35_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK6910635.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="LNE35_001471"
     CDS             27973..28209
                     /locus_tag="LNE35_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBK6910636.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="LNE35_001472"
     CDS             28352..30325
                     /locus_tag="LNE35_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBK6910637.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="LNE35_001473"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="LNE35_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBK6910638.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="LNE35_001474"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="LNE35_001474"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBK6910639.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="LNE35_001475"
     CDS             33204..34496
                     /locus_tag="LNE35_001475"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBK6910640.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DAFCNJ010000025        34330 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000025 DAFCNJ010000000
VERSION     DAFCNJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.026"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            202..1077
                     /locus_tag="LNE35_001476"
     CDS             202..1077
                     /locus_tag="LNE35_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910641.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="LNE35_001477"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="LNE35_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="LNE35_001478"
     CDS             complement(1642..2973)
                     /locus_tag="LNE35_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6910642.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="LNE35_001479"
     CDS             complement(3122..3835)
                     /locus_tag="LNE35_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6910643.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="LNE35_001480"
     CDS             4010..5434
                     /locus_tag="LNE35_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6910644.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="LNE35_001481"
     CDS             5733..6440
                     /locus_tag="LNE35_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK6910645.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="LNE35_001482"
     CDS             6427..6750
                     /locus_tag="LNE35_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK6910646.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="LNE35_001483"
     CDS             complement(6839..7876)
                     /locus_tag="LNE35_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6910647.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="LNE35_001484"
     CDS             complement(8197..9180)
                     /locus_tag="LNE35_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBK6910648.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="LNE35_001485"
     CDS             complement(9595..10365)
                     /locus_tag="LNE35_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910649.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="LNE35_001486"
     CDS             complement(10358..11293)
                     /locus_tag="LNE35_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910650.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="LNE35_001487"
     CDS             complement(11609..13258)
                     /locus_tag="LNE35_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK6910651.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="LNE35_001488"
     CDS             complement(13375..15978)
                     /locus_tag="LNE35_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBK6910652.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="LNE35_001489"
     CDS             complement(15990..17039)
                     /locus_tag="LNE35_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBK6910653.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="LNE35_001490"
     CDS             complement(17065..17379)
                     /locus_tag="LNE35_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBK6910654.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="LNE35_001491"
     CDS             complement(17395..17841)
                     /locus_tag="LNE35_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6910655.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="LNE35_001492"
     CDS             complement(17842..19764)
                     /locus_tag="LNE35_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBK6910656.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="LNE35_001493"
     CDS             complement(19976..20872)
                     /locus_tag="LNE35_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6910657.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="LNE35_001494"
     CDS             complement(20865..21545)
                     /locus_tag="LNE35_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6910658.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="LNE35_001495"
     CDS             complement(21546..22328)
                     /locus_tag="LNE35_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910659.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="LNE35_001496"
     CDS             complement(22321..23238)
                     /locus_tag="LNE35_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6910660.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="LNE35_001497"
     CDS             complement(23402..23725)
                     /locus_tag="LNE35_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910661.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="LNE35_001498"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="LNE35_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBK6910662.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="LNE35_001499"
     CDS             27853..28167
                     /locus_tag="LNE35_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6910663.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="LNE35_001500"
     CDS             28180..28554
                     /locus_tag="LNE35_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6910664.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="LNE35_001501"
     CDS             28564..28899
                     /locus_tag="LNE35_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBK6910665.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="LNE35_001502"
     CDS             28982..30322
                     /locus_tag="LNE35_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK6910666.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="LNE35_001503"
     CDS             30400..31074
                     /locus_tag="LNE35_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK6910667.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="LNE35_001504"
     CDS             31067..31231
                     /locus_tag="LNE35_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910668.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            31307..32449
                     /gene="dcm"
                     /locus_tag="LNE35_001505"
                     /pseudo
     CDS             31307..32449
                     /gene="dcm"
                     /locus_tag="LNE35_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            32439..32729
                     /locus_tag="LNE35_001506"
     CDS             32439..32729
                     /locus_tag="LNE35_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910669.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /locus_tag="LNE35_001507"
     CDS             32988..34172
                     /locus_tag="LNE35_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6910670.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34169..34306
                     /locus_tag="LNE35_001508"
     CDS             34169..34306
                     /locus_tag="LNE35_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBK6910671.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DAFCNJ010000026        32815 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000026 DAFCNJ010000000
VERSION     DAFCNJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.027"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(276..809)
                     /locus_tag="LNE35_001509"
     CDS             complement(276..809)
                     /locus_tag="LNE35_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBK6910672.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1673..2425)
                     /gene="xth"
                     /locus_tag="LNE35_001510"
     CDS             complement(1673..2425)
                     /gene="xth"
                     /locus_tag="LNE35_001510"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBK6910673.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            2538..3458
                     /locus_tag="LNE35_001511"
     CDS             2538..3458
                     /locus_tag="LNE35_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6910674.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3531..4466
                     /gene="murB"
                     /locus_tag="LNE35_001512"
     CDS             3531..4466
                     /gene="murB"
                     /locus_tag="LNE35_001512"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBK6910675.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            4764..6242
                     /locus_tag="LNE35_001513"
     CDS             4764..6242
                     /locus_tag="LNE35_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6910676.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            6519..8579
                     /locus_tag="LNE35_001514"
     CDS             6519..8579
                     /locus_tag="LNE35_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBK6910677.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            8662..9231
                     /locus_tag="LNE35_001515"
     CDS             8662..9231
                     /locus_tag="LNE35_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6910678.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(9406..10212)
                     /locus_tag="LNE35_001516"
     CDS             complement(9406..10212)
                     /locus_tag="LNE35_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910679.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            10350..11864
                     /locus_tag="LNE35_001517"
     CDS             10350..11864
                     /locus_tag="LNE35_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBK6910680.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(12267..13250)
                     /locus_tag="LNE35_001518"
     CDS             complement(12267..13250)
                     /locus_tag="LNE35_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBK6910681.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(13255..13902)
                     /locus_tag="LNE35_001519"
     CDS             complement(13255..13902)
                     /locus_tag="LNE35_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6910682.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(13987..14619)
                     /locus_tag="LNE35_001520"
     CDS             complement(13987..14619)
                     /locus_tag="LNE35_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6910683.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(14635..15186)
                     /gene="coaC"
                     /locus_tag="LNE35_001521"
     CDS             complement(14635..15186)
                     /gene="coaC"
                     /locus_tag="LNE35_001521"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBK6910684.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(15183..15920)
                     /locus_tag="LNE35_001522"
     CDS             complement(15183..15920)
                     /locus_tag="LNE35_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBK6910685.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(15917..16906)
                     /locus_tag="LNE35_001523"
     CDS             complement(15917..16906)
                     /locus_tag="LNE35_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK6910686.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            17164..17706
                     /locus_tag="LNE35_001524"
     CDS             17164..17706
                     /locus_tag="LNE35_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6910687.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            17723..18808
                     /locus_tag="LNE35_001525"
     CDS             17723..18808
                     /locus_tag="LNE35_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910688.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            18820..19626
                     /locus_tag="LNE35_001526"
     CDS             18820..19626
                     /locus_tag="LNE35_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910689.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            19627..20454
                     /locus_tag="LNE35_001527"
     CDS             19627..20454
                     /locus_tag="LNE35_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6910690.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            20451..21524
                     /locus_tag="LNE35_001528"
     CDS             20451..21524
                     /locus_tag="LNE35_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6910691.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            21952..24648
                     /locus_tag="LNE35_001529"
     CDS             21952..24648
                     /locus_tag="LNE35_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6910692.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            24725..25213
                     /locus_tag="LNE35_001530"
     CDS             24725..25213
                     /locus_tag="LNE35_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6910693.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            25225..25719
                     /locus_tag="LNE35_001531"
     CDS             25225..25719
                     /locus_tag="LNE35_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6910694.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            25740..26549
                     /locus_tag="LNE35_001532"
     CDS             25740..26549
                     /locus_tag="LNE35_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6910695.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            26546..27367
                     /locus_tag="LNE35_001533"
     CDS             26546..27367
                     /locus_tag="LNE35_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6910696.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            27418..29517
                     /locus_tag="LNE35_001534"
     CDS             27418..29517
                     /locus_tag="LNE35_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBK6910697.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            29514..30632
                     /locus_tag="LNE35_001535"
     CDS             29514..30632
                     /locus_tag="LNE35_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBK6910698.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            30671..31549
                     /locus_tag="LNE35_001536"
     CDS             30671..31549
                     /locus_tag="LNE35_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6910699.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            31740..31892
                     /locus_tag="LNE35_001537"
     CDS             31740..31892
                     /locus_tag="LNE35_001537"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK6910700.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT        10559 a         5623 c         6772 g         9861 t
ORIGIN      
        1 acctcttggt aaacggtgtc caaaagcttg catctgcggt aataagtcca accaaatgaa
       61 aaatatgaga agctattttt gtttggctgt tcttattact tgatgcagaa cagctttttc
      121 acaacctctc caataaacgg tgttctcctg ccagctcctc ggtcaccagt cacaatattt
      181 aataaacatg agaagctatt ttttgaaatt gctccttgtg actcggagtg aaacggcagg
      241 ctcacgacct ctttgtgtgt ttcataagta ttcttttact ttatagtcac aagttttttc
      301 aatggatctg tcccgaattt tttttcttga taaagcagga tttccgcaca tgcacgacta
      361 tcttccaatg catcatggtg atgatctaat tggatgttca aattttcgca cacagtgttc
      421 aatcgatgat ttggaaattc aggaaacagg cgacggctac ttttcactgt acataaagaa
      481 agataattag gctgttcaag atgataataa tctaagcatc ccgctaagac ctttgtatca
      541 aaagcagcat tatgtgcaac gactaaacga tttttttgaa aatatggttg gatagtttgc
      601 cagacttctg ggaatttcgg cgcattgcgt acgtcttctt gatggattcc gtgaatttgg
      661 acatttcgcc agaaaaaagg agtctcaggc tgtatcaatg tatagaattc atcaacgatc
      721 tggctatttt ttaccatgac tagcgcaaga gaacaagcac tataaggctg atgattggca
      781 gtttcaaaat ccatggcaac aaaattcatt tctgtaaaac ctccttggga aattactgta
      841 acatactttt gaaaaaagca aataaattta acaaaaaaca tagaattttt tatgtaatat
      901 ttgttgagaa aagctagttt ttgaactcat tccttgtgat ttggagttag atggggatca
      961 tgacttctcg gtattaagtc aaatttatgc aaagttgtga ggaaccattt tcaaaaaaat
     1021 ttcttacgac tcgaaagtag gcgacttttt cacaacttct ccactaaacg gtgttcaatc
     1081 agctgaccct cggtattagc tcaaaaaata gcaaaatatg aggaactatt ttgccatttg
     1141 tttctctaat actcaggtca ggacgctgat ttcacaactt ctcaataaac ggtgttcaaa
     1201 agcttgcatc tgcggtaata agtccaacca aacgaaaaat atgaggaact atttttgttt
     1261 ggctgttctt attacttgat gcagtgcagc tttttcacaa cctcttaata aacggtgttc
     1321 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg
     1381 tttggctgtt cttattactt gatgcagtgc agctttttca caacctctta ataaacggtg
     1441 ttcaaaagct tgtatctgcg gtaataagcc caaccaaacg aaaaatatga ggaactattt
     1501 ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaatctc tcgataaaca
     1561 gtgttcaaaa gccagtttct cggtattatg ccaaatttat gcaaaatgtg gaaagccatt
     1621 ttcacaaaat ttttcttaat actcgaaact aaacgacttt ttcacaacct ctttatttca
     1681 aatctaattc tactggacaa tgatcactgc ccataatgtc cgtatggatc tttgcatcag
     1741 ctagtcctgg ctctaaatct ttgctcgtca aaaagtaatc gatccgccaa ccagcattgt
     1801 ttttccttga attgaatcgg tagttccacc aagagtagac accttcctgg gttggataaa
     1861 aataacggaa tgtatcgata aacccgttat ccaacagtcg agaaagagcc gctctttctt
     1921 ctggagtgaa tccagcattt ttttgattcg ttttccagtt tttcaaatca atcttttgat
     1981 gagcgacatt tagatcccca cacacgatga ctggtttttg tttttttaga ttttccaaat
     2041 aattataaaa ggcttcttcc cattctaatc ggtaatctaa acgttttaat tcgctttgag
     2101 aatttggcgt gtaacaggtt acaagaaaga aatcggaata ttccaaagta atcagacggc
     2161 cttctgtatc atggatatcg atacccattc cgtaagtggc attcaatgct ggttttttag
     2221 caaaaatcgc tgtgccggaa tagccttttc gttccgcgta attccaatat tgataataac
     2281 ctggaagatc taaatcaatt tgtcctgctt ggagtttcgt ttcctgcaga cagaaaaaat
     2341 cggcgtctaa ctcatgaaaa acttctaaaa aatttttatt tacgatggca cgaagcccgt
     2401 ttacgttcca agagataaat ttcaataaaa gcacactcct tcgaattaat ttccttacct
     2461 ttgcgatact attgtacctc attttagtaa aaaatggtgt aagatgaaag taactaaatg
     2521 atccaggagg aaagtagatg aagattggtg taatcggtgt agggaatatc gcagaaaaag
     2581 catatttacc aacttatgcg aaaaaacaag gaacacttga tttttatttt gcaacaagaa
     2641 ataaagaaac aaaagaaaga attcaaaaag agtacgggtt ccaacatcta tatgaaacga
     2701 tcgatgagct tttagaagaa aaaatcgaag catgtatgat ccacgcagca acaaaagtac
     2761 attatgaatt agccaaaaaa tgtctagaaa atggtatcca tgtttatatt gacaaaccac
     2821 tgagtgtcca tctaaatgaa gttcgtgaat tgcaagaatt agctgataag aaccaaataa
     2881 ttttgatgat cggtttcaac cggcgctttg ctcctatggt agaagaattg aaacgtatcc
     2941 cagataagcg gttgatccac ctgcaaaaaa atcgaatagc ggcaaaagaa acgacagagt
     3001 tcgtccttta tgatttgttt ttacatctta ttgatacagc tgtgtattta ttagatgaac
     3061 ccattttgag aacaaaatac cagataagag aaacaaagga atttttggaa tatgccattt
     3121 tacaattaga aacacaaaat caaacggcta ttttaacgat ggatttagca agtggcgcta
     3181 atatagaaaa atatcaagta acaagtaaaa atggtactta tgaagtaaac gatctgaccc
     3241 agcttgtcat tcaagaggct gatggaaaaa aagtgaaaga atttggcgac tggacaaaca
     3301 cgctagtcaa acgcgggttt gagccaatgg tagaggcctt cttttctcag atccgtttag
     3361 gtaaatcgga aacagaattg cttaaacaaa aagaagttgt aagaagtcat gaattatgtg
     3421 aggaagtgct taaagatcac taccgtcatc aactttgaac aagactggca agattgtatt
     3481 aagcgtgata gaataatgag gacgtttgag aaaagaaagg aaaatcctca gtgaataaag
     3541 aagaaatagt tgaaagctta gccacgatta ctatattgtt tgatgaacca ttgatgaact
     3601 atacgtttac gaaaacaggt ggaccagctg atgtccttgc attcccgaaa aaacaagaag
     3661 aagtaaagca aatcatcgat tattgccgaa tccatgatat accttggatg gtattaggaa
     3721 atgcaagcaa tctgatcgtt caagatggcg ggattcgcgg tgtagtcatc atgctgactg
     3781 aaatgaaaca gattcatgtc aaaggaacga tggtcattgc ggaagcaggt gcctcactga
     3841 ttgatacgac ttatgcagct ttggcagaat cattgactgg atttgaattt gcttgcggca
     3901 tcccgggaag tgtcggtgga gcggtttata tgaatgcagg tgcgtatggc ggagagatca
     3961 aagatgtttt tgctgaagtg gatcttttgc ttgaagatgg aacattgaaa actttgacaa
     4021 aagacgaaat ggtattttct tatcgtcaca gtaaagtcca agaattacgt gctatcgtat
     4081 tggaagctcg attcagtttg caaacagggg attatgaagc aatcaaagca agaatggatg
     4141 agttgaccga attgcgtcaa tcaaaacagc cattagaata cccttcatgc ggaagtgtct
     4201 ttaagcgccc agtaggacac tatacaggtc agctgatcca acaagcagga ttgcaaggcc
     4261 tgaaatgggg cggtgcacaa gtctctgaaa aacatgctgg attcatcgtc aatatcgatc
     4321 atgcgacagc gacagattat attgaattga ttgcgcacat ccaagaagta atcaaagaaa
     4381 aatttgatgt gtctttagaa acagaagtgc ggatcattgg agaaaaacaa gcaatatcag
     4441 aaacaagcag aagtttagcc aattaataag taatggaact taaaataagt aaaagagcag
     4501 gacggtggcc gtcctgctct tttttgtggt tgtttacgca aatggcgcaa atcttgtaca
     4561 ttttaacaca ataaaacaaa aaatatagaa tatttcctca taaatttttc cattttttgc
     4621 aatattctca aaaaaaagat aaaaaatgag aataactgat gaaaaaacaa gcataatcca
     4681 tcataaaatc agtataggtg aaaagatgaa ctttcatctg catatcaagt aattggtatg
     4741 aggcattttt tgaggtgtag gatatgaagt tggattacct tatagaagag gaagatagag
     4801 aaaaactgct gctagctaga tatttagaat ttgagaaaaa gaattatgta gtgctgccta
     4861 agttatatga aacattaggt ttgtctaaat ttaaaacaag aaattatatc atagaattaa
     4921 ataaagaatt tactgaattc aaaacaaagc cagagatcca aatttcagaa gaagcagaga
     4981 tccatgtcga acgtctgaat ttgacggata tcaaaaattt aagaagaatc tactttcaac
     5041 gatcaaaaat tgctcgactg ttaacggaga tcgtggattc aaaagtaacg atcgaaaaat
     5101 ttgcagagaa tcatttttta agcatatcaa gagcctatgt aaaaagaaga gaattagtct
     5161 cttttcttga acagcatcaa ataaagttaa agaaaaacat attagttggg gaagaaataa
     5221 acatacggaa cgtattattt ggggtatatt tcggggtctt taatggtttt tctatgccat
     5281 ttagagaaag cgtcatgaat gaagcaaaga gtatcgtgca atattttgaa tatctgttca
     5341 atttaaaact gtctgcaaca aaacgaatca aattcaattt gctaaccact attagtttgt
     5401 tgagggccta tgggaatcaa ccaatcggaa aacgtttctt ttcagatgaa tactttaaga
     5461 gctcagaagg aatgaagatc atcaaggatt tatcacatat ttcttttctt catatcccta
     5521 aagagcagat cattgatgag atttcttata tcatgttgtt tttgatcacg gaagaagaat
     5581 ttgaattaga tggcgcattt ggacttgcgg aagctcattt tcatgatatc cacatgatta
     5641 gtcaaaaaat gtcagtggaa ttgattgatc aattaccatt ttcgattgaa atctctgaag
     5701 aagaaagact atatatacga aaagaacttc gtatgggtat gatgcatgtc aatcgaaaaa
     5761 attggatatt cgactttgag atgactagct ttacgtctaa aaagcagcta cagtttttct
     5821 tcgagacgta tccaatattc agtacagcaa tccaaaaggt catacaagag aataaacgct
     5881 atttcttggg aaaagatgaa cgtttcatga ttcgcttatt ttatgattat ctctttttat
     5941 tggtcggtat ttgtccggta tcatattttg aagcaccgat tcatgtctgt attgattttt
     6001 cgcaaggatc tgcttatacc gattacatta tttctcaagt gaaaggattt aaaaattaca
     6061 atattgtgat tgagagtcga atcacttctc gtacgaatat ttatttatct gattgtttac
     6121 ttgaaaaatt taataaagaa cagataattt ggaaaaatcc accgactcca gatgactggg
     6181 aatattttgg aaataccatc atcagagaaa aaaatagaca tttaactgaa aaaatcagct
     6241 agtatttatg gatggcagct ttgtctcaca tatttattag ctgatttatg tttttttcaa
     6301 agaaaaggta actagatata actagatata actagatata ttacttaaca aaattaaaaa
     6361 gtacataagt tattattccg attttcttga aacaataaaa aatcaaaaaa aatactctcc
     6421 taatagtcca tcttcttttg attttattta tataatgaaa tcatcttatg aaaattacta
     6481 cttcatgttt agacaagagt agggaggact aagaataaat gtgttcaaaa agacgttcaa
     6541 aacaactaaa taaacacgct tgtcgtttag ataaggaacg gtcgtgttct gaaaaacact
     6601 acgttaagtt tatgatgcgc acgatgctga tcagtttatc tgtaggtgtc tatatgcatg
     6661 tacccgttca ggcaacgaca caaaccatca aggtcgtacg tacctctgtt tctgagacca
     6721 ctgattcccc tatcactatt actcatactt tcactgatgc aaatggaaaa cagctacttc
     6781 ctgcattgac ttcctataat aaagtcagtt atcctccaac gataaaccac tactggttaa
     6841 ttcctaacaa aacattatgg acatcggaaa ccggaagcag tgtgccgctt tctatctttt
     6901 ccgatatgac tgatcctaca aaaggtgatc agttaaaaca gatcactgat attttgaatc
     6961 atgctttgac agatataaag gacggtggaa gcgcacaagt ttcttatgta tatgaaaaag
     7021 attattcaca gtttgaaaga aagaaaatcg atgttctggc agggacaaaa gtttcgatat
     7081 ctgatctggt agaaaggctg aagaacgcag atggaacaca aggagatatg tccaaagtaa
     7141 tttcaaacaa tgggaatatc gtggatacga ctaatacagg aacatctact atttccttgt
     7201 cctattttga tccaattgct ttaaaaacaa tgactgcatc tgctgtgatc catgttcgtg
     7261 ctgatcaaac agctatcaaa ggaaaagatc ttcacttaag aacaggagag agttacgccc
     7321 ctaaagatct tgttgaatat gtaacaaaaa gcgatggaac ttcaggaaat aaagaagaaa
     7381 tcaaaatgaa cgggacagta gatactacaa ctccgggaga atatcccgtt gtattaactt
     7441 atatagactc gaaaacatca aatgtggcag aaacaacagc tgtagtttat gtagaagcat
     7501 ctgctgcttc atcgactgtc atcactcatt ttatagatag tcaaacaaat aaagaacttc
     7561 aagcagaaac acatatgagt agtgtaaatc gtattcctga tttgcgccta cctgctgaat
     7621 tttctgatcg agtgccaaat atagatttat cgacttatga acaaaatggg aaagtcactt
     7681 ctatttctga ttggatgcaa gcaaacggat tagacagaaa aagtcactgg tcggagatcc
     7741 ttgaagtact tcactcagaa ctgatgaata ataggaagca atcaggaggg gaaattacct
     7801 ttacttatgt ttatatacca gacaatcgtt cattagaagg tcatgatctt attgttcatc
     7861 caaacgaaaa aataaacaaa gaagagttga tccaatcagc aacaaattcc ttaggtaaac
     7921 ccgtaccgtt aaaggatgta tcggttacaa tcaatgaagt accattggag gatggttatt
     7981 tgaccaaaca aacaggtgat tttacgatta tgtatagtta tcatgacccg tattcactcc
     8041 aagaactcca tgctcagtca ctattacatg ttttaccatt aaacaaacag atagatcaaa
     8101 aagaacaaaa aagacgagaa gaaagcttgt cagaaaaaaa acaacaaatg gctcatgcag
     8161 tttctccaac agggaaagta aaacaaacaa gaagcctaga gttagcaaga atatctagaa
     8221 aaatctcaga atctaataaa acgataaggc aagaagctca tttggcccca caagtttctg
     8281 gattggaaaa tgaaatttta gagcctaaaa taaaacaaca atcagaaaaa gaatttgttg
     8341 ctaaacagtc ggaagatcag aaggaatcag atgaccagaa ggaaacgaag aaaaaacgct
     8401 tgtcaactat tgattccacc gatgagcatc tgcagccgca agtaccactt cctagtccgg
     8461 gaacacccgc acctggtgga ggtattcctt cttctgcttt aggagatttc atgcgaggta
     8521 tggcaggaac atgggtatac gctaatcgaa atgaatttga cgcagatgac tggttctgat
     8581 ttatatctct atctgttata gtcaaataag tagctgagtc atatgttatt tggcataatg
     8641 gagacgagga gggatagaaa aatgttagat aaaacgttac cttacgcaga aatctggatg
     8701 acaagaccac ttcaacaaaa tgttccaatc acgcctttag cggaaggttt tgaatttcga
     8761 ttttatcaaa aagaagatga atctgcttgg gcggagattg agacttccgt tggggaattt
     8821 caaacgacag atgaagcact tgagtatttt caacaaaact ttgctcctta tcctgaagaa
     8881 cttgaaagaa ggatgttttt tgtagagaca aaaaaaggag aaaaagtagc aacctgtacc
     8941 gcttggcgaa aaaaatcaag agagcaagtg tatccggttt ttcattggtt agcagtgaaa
     9001 ccagattacc aaggaaaagg gctcgcaaaa gcattgactg ccagagtctt gcaagaattt
     9061 cctaccctcc atacggacgg accaatctat cttcacacac agacatggag ccatcaggcg
     9121 attgctcttt ataaaaaaat gggttttact tttattgctg aaaatctaga cggcacacct
     9181 aatccagatt atgaaaaagt tatgcaggtt ttaagtaaat tagataaata acgaaatggt
     9241 gaaataagtc ctcagtattg ggataaaaaa cgaaatagtc attcctcata tcttactttt
     9301 ttgagttaat actatcggat aactaatggc atgccataca ttcaaaaaag aaggggcagt
     9361 gacaaaagta ttgtcactgc cccttctctt gttttttagc atgttttatc gtttgatttt
     9421 tttaggaata aaattcttga tgaacgtaaa tttttggtaa tcgtttcgac tatagaggta
     9481 accaaataca ctgaaaacaa ctgctccaat aaaattaaca aataaatcct tcatagtatc
     9541 catgattccc acgtctaagt aaccattttg gatgattgtt ttatctgttt taccatctgc
     9601 attttgactt tcaatgattg tccgagtgat atctggaaca tgatagacag tgtttccgtc
     9661 tttacttaac tccacactat tgatttgctg gatgatcgta tctttttgca tatcgaattg
     9721 cagtaattta tctgctgaaa attccacaaa ctcccacata acaccaactg tcatcgaaaa
     9781 acagaaggaa acaactgcta gtaataaagg agtcaagttc atggtttttg tatttttatt
     9841 gagcaaataa acaagggaaa agccgacacc cccagctaaa aatccgctga atgcatgtag
     9901 aaaagtatcc cacattgaaa aatagccata aaaatcactt aactctccta aaactgttga
     9961 actaaaaata aaagataaca aaatgatttc tagcatattt ggaatctgta tctttaatat
    10021 cttttccaga aaataaggga tttcaaataa aattaatgtc agtaataaaa gcattgtatt
    10081 tccccagtgt tggttccaga cctgatccac tagcataata acgacgatga tacgtaataa
    10141 aatatagatt ccgtttgtga ttctacctgc agtttttgta ctgttcgtaa aaaaggtttt
    10201 caaatgattc ataaaataac tccttcatgt atatccttaa ttatactaag aagcgtgtgt
    10261 ttaccaaagc tatatgactt aaagaaaaac agagttgata ttgtgaaaaa aatcacttaa
    10321 tatagtataa ggagaaaagg gggatacaaa tgaaaaaatt agttgctggt ttattaacag
    10381 ccgttgcact tcttgctatg agcgcatgcg gcgcagatca gaaaaataca gcgcagacaa
    10441 gttctagtac gaaggaatct gccgaagtat catcaggcgc ttctgaacaa gtatatacag
    10501 atccaaaaga aatgaaagaa tcttatgatg tcgtgattat tggagctggt ggtgcaggga
    10561 tggcagcagc tatcgaagca aaagatgctg ggttgaatcc agtaattttg gaaaagatgc
    10621 ctgttgccgg aggaaacaca tctaaatcat caagcggtat gaatgcttca gaaacaaaat
    10681 ttcaaaaaga agaaggaatc aaagactcga atgatgcctt ctatgaagaa acgctaaaag
    10741 gtggtggagg aacgaatgat aaagaaatgc ttcgatactt tgtcgatcac tctgctgatg
    10801 cggtcgattg gctagatcaa aacggtatca aactggacaa cttaacgatc actggaggga
    10861 tgagtgaaaa gaggacacat cgtccgtctg atggctcggc tattggtgga tacttagtgg
    10921 aggggttatt gaataacgtc catgagcggg aaatccctat ttttgtcaac gcagatgtga
    10981 cagacattaa gaaaaatggg gaaacggtca atgaagtgac tgtacatgtt caaggagaag
    11041 aaccaaaaga ggtcaccgga aaagcaatcg tagttaccac aggcggtttt ggtgccagca
    11101 aagaatttat tgaaaaatat cgtcccgatt tagctgatta tattacgaca aatcaagaag
    11161 gatcaactgg agatggtatc accatgattg aaaaattagg tggacaaacc gttgatatgg
    11221 ataaaataca aattcatcca actgtgcagc aggatgaggg ggtcttgatt ggtgaggttg
    11281 ttcgcggaga aggagcaatc ttggtcgatc aagaggggaa aagatttgtc aatgaaatgg
    11341 atacaaggga taaagtatct gcagcaatta cagcattgcc tgaaaaatca gcttatttga
    11401 tttttgacca aggcgtgcgt gatcgtgcca cagctattga attttatgac caaaaaggat
    11461 atgtcacaac tggaaagaca atggaagaat tagcagaaaa aatcgattta ccaaaagaaa
    11521 cactagcaaa aacagttgat ggatggaata cggatgttgc ggagaaaaaa gatgctcaat
    11581 tcaaccgtgc cacagccatg gaacatgact tatcgtctcc gaattattat gcgatcaaaa
    11641 tcgcaccagg aatccattac acgatgggcg gtgtgaaaat caatacgaac actgaagtat
    11701 tagataaaga aaaccagcca attagggggt tatatgctgc tggtgaagtc acaggtggct
    11761 tacatggaaa caaccggatc ggtggaaatt cagttgcgga gatcattatt tttgggcgac
    11821 aagcgggcat acaagcagct cgatttgctt cggaaaatga atgaaataga acgtaaataa
    11881 aaagaggttg tgaaatcagc gttttgagct aataccgagg gtcagctgat tgaacactgt
    11941 ttagtgaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaagtaaaa
    12001 acagcttctt atgtttttaa cttggttgga cttattaccg cagatgcaag cttttgaaca
    12061 ccgtttattc ggaaaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta
    12121 aacacgtata aacggcggaa gcagaagaaa ttccttcgga aataagctga aattcacaaa
    12181 aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc
    12241 cagcctcttt tttgatcagc atgattttat tcagttatct tcaaaatgat ttttccgatg
    12301 tttttgtttg cttccatata ttcatgcgct tgtttgactg cgctgaattg gaatgtacgg
    12361 tcaatgattg gccgaatctt gccattttct agaaaaggcc cagctttttg cataaaagtt
    12421 tgtgcgagct ctgctttata ctgatcactt ctaggtgtca ataatgtacc aagcagttgg
    12481 acacgtttcc caatcaatac accaaggtct atctcaggca cgatggtccc acctagcata
    12541 ccaatcaaga cccaacgtcc atcaacggct atagaagcta aattcttttc ccagtaagaa
    12601 gcgccgatga aatccaaaat aacatcgact ccccgacctt cagtgatttt tcgtacctct
    12661 tcttgaaagt cttgtttttt atagttgatc aattcatctg caccaagttc tttacaaaag
    12721 gcgagttttt cttctgatcc tgcagtaacg atgattttcg cgtgagaaag ctgtctagct
    12781 aattggatcg ctgctgttcc cacaccactt gctcctgcat ggatcaagac agtttcgcct
    12841 tcttgtaatt ttccaagcca gtaaagtgtt tgataagcag tcaaaaaaac ttctgagatt
    12901 ccagcagcag aaacatagtc tagcgtatca gggagaagga ttgcacgatc ggcaggcatc
    12961 acagcatatt cggcataacc acctagattg actaatccgt atacttttgt cccaggagta
    13021 aaagtggaga tatcactttt attctcaact acctctccag ctacttcgac tcccaaaata
    13081 gggtagggag gtttcaaaga gactgattct cttttcataa tatctgttcg attgacagct
    13141 gctgtatgga ctttgatcag taattcgcct tgttttggtt taggaacagg cacttcaatt
    13201 tgttctaata catctacaga accaggatgc ttgattgaaa tggcacgcat ggatctattc
    13261 ctcctttgcc cattcttgga atgctgtatt caactctttg ctaccttcag aaagtactag
    13321 agtcgtacga gaaatatccg tttcttccac tagagcggca agtacagcag caactgtaga
    13381 gcgagtgatc gtttttgatg ccttctcatt aggcttagtt aattgaatgg aagtaacttc
    13441 ttcatcgtta gtcaatgtga ctggttgaag aatgacaaaa tctaagttag ttcgtttcag
    13501 ccattcatct gcataatgtt tagtaatata ataatctgtc atggactcag gccattttgc
    13561 tggttcatct gcataaacag cactgaccat cagatagcga gagacatgcg cattttcagc
    13621 tgcgatcact gttttgattg cgccatccaa gtctacttgc agaagattct ttccttgaga
    13681 gccggcagca aaaatcaata catcgatgcc tttgaatgct tcggtcattt tttccacgga
    13741 ccaagttaaa tcaaaagaga catacgaaac attctctttt tttactaatg tttgggaagc
    13801 aacatctctc acccctgcaa aaatttcatg ggaacttgga gctaaatctt ttatcaggtg
    13861 tcggccaatt tgtccgtttg cacctacaac aaaaattttc atttggatca tcctttcgac
    13921 gtcaaatctt tccattcaat agtaacacca aaagagaaaa aaatcagaga tcggctacga
    13981 tgactatcag ttagtaagag aagggtgctt gaagtatctt aacaagacat tgcatactgc
    14041 tgcagtcgca agacctgcga cgattccttc aggtatacca cttgtaaaaa taaccgctag
    14101 aatagctgaa taaacagcat ttaaatttgt tccgatgact ttggcataat cttgctggaa
    14161 taaaaagtag atcatattca tcactaaaat cgtattggtc aatgaaccgg aaagtccagc
    14221 taagaataaa gaaacggaat ctgctttttg tttcattagt ttgcgcagac ctttataaac
    14281 gaaataagga accaccccaa tcaagatgcg aggaataaac gcgatcagca aggctttcca
    14341 gcttccatgt tctgttccga tgactgggat aaacggagaa aagacaaaag acaaaggtgt
    14401 ttggataact gtgctacgga tcatgctgat cagtccaaat gtaccgccaa gaaaagcacc
    14461 tagtcttggt cctaaaacga tcgaagcaat gattacagga atatgcatgg ttgttgcatt
    14521 gattggaccg atagggataa aacctaaagg cgtaacagct aataaaatca aaatagctaa
    14581 aaacatcgca gttaatgtaa agttttttgt atttttcata agtactcctt ttttctaagc
    14641 atttctttca ttaagagtca gcatgacctg ttcgatgatc gtctgaatat cagctaaggc
    14701 accttttcca aaatctccac aagctaaaag agattctctt ggttcaattt cctgatagcc
    14761 gacagatctc aatgtttcca gattacgctg agtgatcggg ttttgataca tattcgtgtt
    14821 catggcaggg gcaatcaatt ttggtgttcc cactggaagc gccattgcga ctgttgaaag
    14881 aagatcgtct gcaatgccgt ttgctagctt gccgataatg tttgctgtag caggcgcgat
    14941 gagaaacaag tcagcttgtt ttgctagttc gatatgattg attacctgcg gatttgattc
    15001 ttgcatgacg tctgtatgga ctgcgcgctt cgacagagat tgcaaggtta atggcgtgat
    15061 aaaatgtgtg ctgcttttag acataatcac atctacctgg gcacctaatt tgacgaattg
    15121 acttgtaaga tcagcgcttt tgtaagcaga aatacttcca gtaacgccta gtaagatctt
    15181 tttcattttg attcctccag tctaattttc tcagtaatca atgcagcaat ctcagattta
    15241 ctttgagctt cttccactgt tccatcttta gataagagat agccgtggtg tgtctcgcca
    15301 tggatttccg tcaaatcatt tgcgaaaata aaatcagttt tgttatttat cagactattt
    15361 tttgcgactg ccagaagaga ttcctttggt acatctacta atagtttgaa accaatgatt
    15421 tttgcttttg gctgccattc acgtagataa gaaatgattt ttggtgtttt cttcaaaacg
    15481 agcacaagat gctcggtgtt agaagagatc tttgaaacag tggtatctgt ctgttcattt
    15541 tccgcaaacc aattgtccag ttcttcttgc gttgaagaag tgggtaggtt ttttgccagc
    15601 tgttcttctg aaaaactaaa agcaggagta aaatcactga ctgccatact gtggataatc
    15661 gcatcgtagt gttctttttt cgttagttct tctaattgta aaaataagtc cagtgcagat
    15721 tcgatcgtat aaatcgttat attggagtgt cttttcggtt tcagtgcttg tttcgtagtc
    15781 acgtaatcga ttgtggtatc cggattggca gctaaataat cagctaatgc ttgccctaat
    15841 cgtcctgtcg aatgatttgt aatagcacgt acttgatcga ttttttcagt cgttcctcca
    15901 gctgtgatta gaatcttcat ttatggtcac ctacatttac tagtgttctt ccttggtgtg
    15961 ttccagcaag tagtttagat gctgtttcag gcaattgatc cagagtaatc tcttggacga
    16021 ccagttcatc tgcaatctgc aaatccgcca tctgttgcca taaagaaaga cgttgatcta
    16081 ttggtacatt gacagaatca attccaatca actggatgtt tcgcagaata aaaggcaaga
    16141 cagtcgcgtt tagtttgatt cccccagcat tgccacataa agctactgca ccgttataag
    16201 agatcaatgg aagaagactg cttagctgct cgccacctac tgtgtcgatg acatagtcga
    16261 tttgctgttt tcctaaagct tttgttgtct ctggcagaaa ttcatcaggt cgtacgactt
    16321 gcaaagcacc tagacttttt agccatgtga cagactcttc ttttctggaa aaggcaatga
    16381 ttgactggaa tcctagttta tgtaacaggg ctatcgctgt actgcctaca ccgccagaag
    16441 caccagtaac gacaactttt ttatcggagg tcataccttg acggattaaa gcgttcacac
    16501 acaacatagc agtaaaccct gctgtaccaa ggatcatcgc ttgacgcagc gtcatatttt
    16561 ttggtagagg aactaaccag tctcctggta ctttttgata ttggctataa ccgcctggat
    16621 gggtgacgcc aagcccaaaa ccagtagcaa gtacaagatc ttcttttgaa aaacgtggat
    16681 tgttactttg gacgacagtt ccggataaat caatccctgg agtcatcgga tatgttcgta
    16741 tcacctgacc agactcgctc attgccaatg catctttata attgacatca gaataggcaa
    16801 tttttaccaa gacttcattt tcagccaact cagtaaatgg cagatcactg attgctgtat
    16861 ggaaaacagg ttcctttatt acctgaaaag atcgaaaagc gttcattcgt gagacactcc
    16921 ttttaaaatt ttcttttatc ataccataaa gcctactctt ttattcttgt tcttccgtaa
    16981 atttagacaa aaattaggtg aaaaataaag aatcaaacaa ctagaattta ttccaaaaga
    17041 taaagaaata ttcagcaaaa aaaattttac aaagggttgc ctttttcaat aaatttcgta
    17101 tactaatacg agtggtagta aacaggaagt ttactattag aaaacttcga ttcggaggaa
    17161 attatggaaa ttggtgagaa gttaagaaat ttgcgcatcc aaaagaattt gacacaagaa
    17221 gaactaggcg aacgcacaga tttgtcaaaa gggtatattt cccagttgga acgagattta
    17281 agttcaccat caatggaaac attcttttct attcttgaag tcttaggtgt gactccagaa
    17341 gaattttttc atcaagaagc agcgaatctg caagtggttt attcaaaaga agatcatacg
    17401 gtttatttcg atgaagaaaa cgggtatgaa ttggaatggc tcgtggctga ttcgaatgaa
    17461 aaagaaatgg agcctgtctt gctgactttt gaaggtgcag gagaatataa gacgtttgaa
    17521 ccttcattat ctgaaacgtt tatttttgta ttagaagggg aattagagtt aagcttagga
    17581 gagacggtct atgtggcaaa agcaggacag tcgatctatt atcaagcgac aaaacaacat
    17641 caattaagga atcatctcaa aaaacgaaca aaagtattga ttgttgcaac gcaatcatat
    17701 ttgtaatata gggggtaaaa acgtggggaa accaatcatt tcatttgatc aagtagtgaa
    17761 acgctatgat gatgaaacag tattaaaaaa agtaagtttt gagatcgaac aaggaaaatt
    17821 ttatacactg cttggtcctt ccggctgtgg taaaacaacg atcctacgta ttatagctgg
    17881 attcaccgaa gctacagaag gggacatcta ttttgaaggg aaacggttga atgatttgcc
    17941 agctaataag cgacaagtaa atacagtctt ccaagattat gcactttttc ctcatatgaa
    18001 tgtttttgat aacgttgctt ttggattgaa gatcaagaaa atgtccaaat cagatatcga
    18061 aaagaaggta aaagatgctt tgcgtatggt ccagcttcct ggatacgaaa aacgggagat
    18121 tagtgaaatg tctggtggtc aaagacagcg tgtagcgatt gcacgagcaa ttgtaaatga
    18181 accgaaagta ttgctattag atgaaccgtt atcggcgctt gatttgaaac tacgtacaga
    18241 tatgcagtat gaactgcggg atctgcaaca acgattagga atcacgttta tttttgttac
    18301 tcatgaccaa gaagaagcgt tggcaatgag tgatgagatt tttgtcatga ataaagggaa
    18361 aatcgtccaa agtggtacac ccgtagatat ttatgatgag ccaatcaatc atttcgttgc
    18421 tgattttgtt ggggagagca atatcgtgga cggtgtaatg atcgaagata atctagttga
    18481 gtttgttggt aagcaatttg aatgtgtcga tggcgggatg cgtctaaatg aaccagttga
    18541 agttgtttta cgcccagagg atttgacgat cacgacacca gataaaggaa aactagtcgt
    18601 aacggtcgat actcagctgt ttcgtggtgt ccattatgaa atcatctgtt atgatgaaca
    18661 gcaaaatgaa tggatggtcc attcaacgaa aaaagctaaa gaaggaagca aagtaggatt
    18721 ggcctttgag ccagaagata tccatgtcat gcgtttcaac gagtctgaag aagaattcga
    18781 tgcacgtctt gacagctacg aagaataggg gggacaagaa tgaaaacaat gcgcaggatc
    18841 tattccgtcc cttatatttt gtggctcggt ttattcgtga tcgcgcctgt attaatgatc
    18901 atttatcaat ctttttttga tatgaatggg caattcacgc tgaataatta tctatcttat
    18961 tttacgtcgg ggacatcgct ttctatgacg atcaattctg tatggtatgc atttttgatc
    19021 acgtttttca cgctgatcat cagttacccg actgcttatt ttttaacaaa actgaagcac
    19081 aagcaattat ggctgatgct gattattttg cctacatggg tgaatctgct gcttaaagct
    19141 tacgcattta ttgggatttt cagtgtgcac ggcagcgtga atcatttctt tgattttatc
    19201 gggatcgggc cgaaacagat attattcaca gacttcagtt ttctgttcgt ggcaacgtat
    19261 atcgagattc catttatgat tatgccaatt ttcaatgcat tggaagagat gaatccttct
    19321 ttgatcagtg ctagtagaga tttaggcgcg aatagctttg agaccttccg acgagtgatt
    19381 tttccgttat cattaaatgg tgtgaaaagt ggtgtccaag cagtatttat tccttctctt
    19441 tctttattca tgctgactcg tctgattggc ggaaatcggg tgatcacttt aggaacggct
    19501 atcgaacagc atttcttggt tacacaaaac tgggggatgg gttctacaat cggtgtgatt
    19561 ttaatcattg ctatgttcat cgtgatgctc ctgacaggcg agaagaaaaa aggaggtcgc
    19621 tcataaatga aaaaatttaa atggtcgtat ctttatttag ggatcgtctt tttattctta
    19681 tatgcaccga tcttctatct gattttttat tccttcaatg aaggaggaac gatgaacagc
    19741 tttacaggat ttacttggga aaattatgaa gctgtttttg aagatacacg tttgatcatt
    19801 atcgtattga atacgttcat gctggctttc ttatctgcat tgatcgccac gatcatcggg
    19861 acatttgggg ctatgggaat ctattacacg aaaaaacgac gtgcacggac agcgttgttg
    19921 agcatgaata acatcttatt agtctctcct gatgttatta tcggtgctag tttcttgatt
    19981 ttctttacac tactaggatt cggattaggt tttcagagtg tattgctttc ccatattgct
    20041 ttcagcattc caatcgtcgt cttgatggtc ttgccaaaat tgcaggaaat gaacgattca
    20101 atggtcgatg cggctagaga ccttggtgct aataatttcc aagtagtcaa aaatatcatt
    20161 ttgccttatc tttctccagg aatcatagct ggatatttta tggcatttac ctattctctt
    20221 gatgattttg cggttacatt tttcgtcaca ggaaatggct ttacgacgct ctcagtagag
    20281 atctattctc gtgcaagaca aggaatcagt ttagaaatca atgcgctaag tgcgttagtt
    20341 ttcttgttct ccatgatatt ggtcattgga tactacttta ttagtaaaga aaaccgtcca
    20401 aaacggctga agaaaaacag aagagtacgc gagggggtgg cacggctgcg atgaaaaaat
    20461 tacaatctct gtttttaggt attctcatca ttattgcggt gctgctgctt ggtgttcgtc
    20521 aacttgaaaa aacaagtggc atgagtggag caaaggtact taccatctat aactggggag
    20581 attatatcga tcctagtttg atcacgaaat ttgaaaaaga atatggatac aaagtgaatt
    20641 atgaaacatt tgattctaat gaagcgatgt tcacaaaaat ccagcaaggt gggacaaact
    20701 acgacatcgc tataccaagt gaatatatga tccaaaaaat gatcaaagaa aaactagtac
    20761 ttccattaga tcattcgaag atcaaaggat taaataatat tgatccacga tttttagact
    20821 tggattttga tccagataat cagtattcga ttccatattt ttggggaact ttagggatcg
    20881 tatataacga taaattcttt gatgcaaatc aaattaagca ttgggatgat ttgtggaagc
    20941 cggaattaaa agacagtttg atgctgattg acggtgcacg agaagttatg gggctttctt
    21001 tgaatagttt aggctattct ttgaatagta agaatatggc acagttgaca gaagcagcaa
    21061 ataaattgaa tcatttaaca ccaaatgtca aagcaatcgt ggcagatgag atcaaaatgt
    21121 atatgatcaa tgaagaagcc tccgtggcag tgacattttc tggcgaagct gccgatatga
    21181 tgtgggaaaa tgaacattta cattatgtga tcccaccaga aggatccaat ctttggtttg
    21241 ataatatcgt tatgccaaaa accgtgaaaa ataaagaagg tgcctatgat ttcatcaact
    21301 ttatgctaga accggaaaat gcggcacaaa atgcagagta tattggttat tctacaccaa
    21361 atgaagaagc aaaagcgtta ttgccaaaag acatttcgtc ggacgaacaa ttctatccat
    21421 ctgatgacac gatctcccat cttgaagtct acaaagatct aggatcgaaa tacctaggca
    21481 tctataatga cctattctta gaattcaaga tgtataggag ataatggcaa aactcagtaa
    21541 gaatggggac ttatcaacgt tctaaagagc taaaaatcgt cctttttcta tctgagtttt
    21601 ccaatcttct accaaaagcg gatgaaacgc cgcttttttt gtgttatttt attttttcta
    21661 ccacgttttt ctaccagaga aaagtggaca aaaccattgt aaaatagggc ccgagacggg
    21721 ctagtaaatc taagattgaa aaagttacag aaggataaag taattggaaa ctatgcaaat
    21781 atacttaacc cttctgtaat ctattaataa aactaatata tgaatccagt tcaatatttg
    21841 tagctatgaa aaaacataaa aatcatatta gataaaaact aaattggtag ataaattatt
    21901 ttgtcagata tctgaggcaa atattcgact atttgtgttt caaagtaaaa attggaatac
    21961 aaatatagaa tagacatatt gcagccgata gtttattatg cttttagaca cattaaactg
    22021 gaggaaaaaa tgaaaaagag cgctaaagat cgagtttttg atgagctgat acgactaaca
    22081 gggaatatgt cttctgtcac tgccgagaaa atttcgaaga atctaggtat ctctcgtcag
    22141 aatgccagcc attatttgac gagattagtg gaagataaaa aggtcgagaa attaccggga
    22201 aagcctgttt tgtggaagcc tttagatgaa tatgcggttg tagataatac ggagcaaata
    22261 aacgaagcct ttcattctgt agttggtcat gacggaagtc tgagagaggt cattcaaaaa
    22321 tgtatagccg ctgtcaaata tccgcctaac ggactcagtg tattaataaa tggagccacg
    22381 ggtgttggaa aaagtttttt agccacaaaa atttttgaat acgctatcca tgagcaaatt
    22441 attgaaaaag atgcaccatt tgctatctta aactgtgccg attatgcgga caatcccgaa
    22501 ctgctttcgg caacactgtt tggatacaag aagggggcct ttacgggggc tgaaaaggat
    22561 accgatggtt tattggcaac ggccaacaac ggctatcttt ttctcgatga ggtccatcgc
    22621 ctatcgaaag aaaatcaaga aaagctcttt ctatttattg atactggaaa ctatcgtcca
    22681 gtcggtgaaa atgtaaattg gcattctgct aaggttcgtt ttattttcgc cacgacggaa
    22741 aaaggtgaaa attatttatt agacaccttt gatcggcgta ttcaaattag tgttatgttg
    22801 ccaacttttg aagatcgccc cattcgagaa cgattagagc tgattcaatt attttttcaa
    22861 aatgaagcgg atgttttaca aaaagatatt attgtttcaa gagaagcctt gtctttaatg
    22921 ttatctcatc cctttagtgg gaatattggt aagctgaaga atatcattaa aatcagttgt
    22981 gcggatgtgt atagtcgaac acaagaagaa ccattaacga ttgggaaaaa tgagatcttg
    23041 attcagttaa atatgctgga atccgaagta gaaagcttgc cgattgctag tttattgata
    23101 aagaaaaatc aaaagataaa aacggatcga atcaaaccgt ttgattatga ttcgattttg
    23161 gaaaaaatta ctcagctgat aaatgaaggt aatttcaatg aaaatttttc ttcgataaag
    23221 aatgaagtac agcatttggt gcatcacatt gataaggatt cttttttatc aatgagtaga
    23281 aaattgatga ttcagatgtt tagaaaggtc tgggtatccg ttttaggaaa aacgtacgga
    23341 ttgacgacga gtggacctgt tgcagaaatt attagcagat cctatgtcat ttccccaagc
    23401 tataagacag acttaaccgg gataaatcgt acattagctg caaatctacc tcggacgact
    23461 tacttatgta atcagttctt ggagcattta ccaccgctct cagaaaagga cgagaacttt
    23521 ctaagactgc ttattgtcgt gactttatct gattatgtag atgaatccat cgagctgaag
    23581 ggcctgttgc ttgctcacgg agatagtacg gctagcagca ttcaagcagt agtgaatcaa
    23641 ttatgcggca attatgtttt tgaagcgttg gatatgccag tagagagtaa tgtgtcagag
    23701 attgtcgaat cgacaaggaa tttcgtgaaa cggcaagtga atacagatag tctagtgctg
    23761 atcgtagata tgggctcttt aaatcaaatt tacacacaga tcaagaacga tttaaaaggg
    23821 gagttacttc ttctgaataa tttgacgact tccattgcac ttgatattgg cttgaagatg
    23881 acgatgaatg cgccatttaa acagattgcg gaggatgcag cagacaagta ttcaatcaat
    23941 gttcaatact ttgaaggctt ttctcaaagt agcaacatta tcatttcctg tatgtcgggt
    24001 ttggggattt cggacaaatt gaaagatgtt tttcgtcatc atttatcgga tgaatccatc
    24061 gaagttttca cgaaggacta cagagaacta cgtgaattaa tcgaccaaaa tgatgaaact
    24121 tattttgaca agacaaaatt ggtcatcacg acttcggatc ttccacgttc cttttcaatt
    24181 cctaatatta atgtttatga catcttagac ggtgatggag cagccaattt ggagcatatt
    24241 ttgagtcctg aaatgaagcc ggcagaattt aaccgcttga ttcaggagct agtaaaattc
    24301 ttttcgttgg aaggaattgc ggaccgtttg aactttctga acccaacgat cgtgatgagt
    24361 gaagtggaaa cagtcatttc taaatatgaa aattactatc atttcacttt aaatggccgt
    24421 gtaaaactaa atctctatat gcatacggca ttgatgatgg aacgtttatt cttggctcgg
    24481 acagaaaaat ctgaagaacc gaattatgat ttgaaaccag atgaagatga attttattat
    24541 gtttctagaa gtatttttca atcaatggag atgaaatata attttaaagt caatgcttat
    24601 gagctttcgc tgctctatga attattagga ccgtatattc aaaaataaag tgtctaaaaa
    24661 agtgtctaat ctgttttttt taggttagac acttttttta atgtgaatta acgtgattaa
    24721 accaatgttt ttaaaagttg gcacaggaat tgcttatata aaagtgaaca gaaagaaagg
    24781 aattaaccat atgaagaaaa ttctaatagc gactcacggt cacttagctt caggctttct
    24841 tagcagtatt caattactga cggggaaggt tcaagagatt acggtgatta atgcttatgt
    24901 ggatgagtgt gattttgaac aggagttgaa tgtatttgta aaccaggtga acgagcagga
    24961 tcagattttt gtttttaccg atttatttgg cggaagtgtc aatcagaaga ttaccaaaac
    25021 ctttttggag aaagagattg ctgtaactat tttgtctggc tttaatctgc caattctgct
    25081 cgaaatagta ttggcaacca aggaattaac aaaggaggat attcaggaac tggtaaataa
    25141 atgccaacag gaattaaaga tgaccattct tgatgaggta catgtagtaa atgaagagga
    25201 agattttttt taggaggaag aaaaatgatt aaagcagtgc gaattgatga acgattaatt
    25261 catggacaag tagcgatgac ttggacaaag agtctaaatt taaccgggtt agtagtagca
    25321 agcgatgagg cggctaccaa cgatattcaa aagatgacat tgaagatggc tgcaccatcg
    25381 aatgtcaaag tcattattaa aacagttgat gacgcgattc atttgttaaa agatccgcgg
    25441 gcgaaggaca tgcgcttgat ggttttagtc cctaatgtca aagatgcggt aaaagttgcg
    25501 aaagctttcc caacggaaat cgaattaatg aacgtgggga atgctgggaa aatgacaccg
    25561 ccatcggaag ataagaaaac cttatcaaag gaagtgatgt taacgacgtc agaaattcaa
    25621 gacttaaaag aattagttga actttatcca gataccgttt ttcagcctac accggcgatg
    25681 gaaaaacgtt cagctatctc tattttgagt gatttttaga aaaaggaagg agaaaaatta
    25741 tgtatgatgc atttgtagtc gctttggccg tttttattgg aattgctgga catgaaatgt
    25801 tcgggatgtc catgctgagt cgtccgattg ttgttgctcc gttagcagga gcgttgatgg
    25861 gggatttgca gacggggttg attgtcgggg cgtcactaga agccatcttt atgggtgtcg
    25921 tcaatatcgg aatctctagt acggcggagc cttctttggc tgctgggctg gctacagcgt
    25981 tcgcaatcaa aatgggagga aacgtggatg cgattattcc cattgtcttt ccactagcag
    26041 tattaggatt acagttaatg aatatgattt tttcttttgt atgtggaccg atggctgcta
    26101 aatttttcaa atttgcaaaa tctggtgatc aaaaaggaat catcaatctg cacttcttta
    26161 tgtggtttgt ccactatgcg ctttatgctc ttattccatt ctttgccgtt ttatttggag
    26221 cagatgttgt caaaagcgtc ttggataata ttccagaggt catcatgaac ggattaacag
    26281 tcgctggaaa tctgctgccg gcagttggga tggcgatgct gttacggatg ctttggagta
    26341 aaaatattgc tgtgtggttt ttctttgggg ccgttgttat ggcgtatctc gagttaccat
    26401 tgattgcaat tgccgcagtt ggaacgatta ttgcaattgt tatagcccaa agagatctac
    26461 aactaaaaaa tatatctgtt catgcagttc aaagcgatgc tgctgtaaat gcaacgcttg
    26521 accaagaaga ggaggatttc tttcaatgaa attagctgat ggaatttcta aagatgagaa
    26581 aaaattggtt cggaagatgt tttggcgctc gatgactatg tatgcttcgg tgaatccggt
    26641 gacgatgggc ggcggtggtt tcgcctattc tatgatgccc ttcattaatc atttctacaa
    26701 aagtgaagaa gatcgagctg cggcgctgga acgtcataca cagtattttt cgacgaccat
    26761 tccttgcgcg tcctttgtca tggggattgc ggcatcgatg gagaaggaaa attctgaaaa
    26821 agctggtttt gacgtggaaa gtatcaacgc gatcaaaacg agtttgatgg gaccattggc
    26881 tggaattggc gattcattgt tctggggagt ttggcgtgtc gttaccgcgg gactagcgat
    26941 caacttagcc aatgccggaa atattttggc accaattatc tttttagtta tgttcaatat
    27001 acctaattac ttgatgcgat actatggcgg cgttttaggc tattcattag gctctaaata
    27061 tattgaaaag ctgtatgcga acgggttaat tgaaattctg acaaaagctg ctagtatctt
    27121 gggtctgatc atggtaggtg caatgacgat cagtatggtc aaattcaata ccgttttgag
    27181 cttttcaatg aagggcgaag aactaatgaa tgtccaagaa atcctcgata gtatttttaa
    27241 aggaattgta ccgttattgg taacattgag ctgtttcaaa ttactagata aaaaagtcgg
    27301 cgtcatcaaa attatttttg gaatggtcgt cttgggaatt ttattgtcat tacttggaat
    27361 cgcatagtag gaagctgcag ctactaggct gcagtttttt tagcaaggag ggaaaagatg
    27421 actattaatg aagtaattta ttcacccaaa cacccatttt ttaatgctga gttaaacgac
    27481 ggaaatgtga ctacttcaaa cgaatacagc tactatcgga atattcctga tgtaaaggga
    27541 ttgaccttcg atgtaagtaa tcaaaagaca ttagcaaacc ttgatgtttg cgggacgatt
    27601 caaacgttga ccttttatcg agaaaattta ctaactgagg aaaaaccagg tgtttgggtc
    27661 aataaacagc tctctcaaac caattcgtta tccttaaagg ttgaagtaaa cgggattgtc
    27721 taccatttgt cagaatcgga tcatcgtatt gaagtcgatg tgattcaaga ctgtttgcct
    27781 agatatattc atcattattc gacgtttaag gtcattgtag tatcctttgc tcccatactg
    27841 gctaataagc gtttaagtat gcttgttcaa caggtttaca ttgtgaatga aacacacgaa
    27901 agccttcaag tttcagtgaa agatgtgcct tcgtatcaag agaagtattc ggatcagcaa
    27961 aatgtgttga tttctcaaaa aggaaataag gacacgatcg cacaacagga agtggcaagc
    28021 ttcgccattg cttttgtgga cccaaatgct tttgaagaag taatgacttt tcaggaatct
    28081 gatatcgaaa aatggctgaa ggaaacattg aactattttg ctcagctatt tgggcagctg
    28141 tcaatgccaa acgatagagt tgtccatctc tatcgtcgtg cactttatca aagcttttct
    28201 agtttcggta tgaatcgaca gggacaggtc gtcggcagca attggggcag ctatcctgct
    28261 actaatcgaa tttggaataa ggatatgtat tacgcatcat tgccattcac catgtttgaa
    28321 ccggagttat gtcaaaaaac ggttctatgg tttgatcaat atggcgttaa gtttcctggt
    28381 tcaaaattta caggaggtat caatcattct ctgagtaatt cgttatccag tgcgttactg
    28441 gctagtcttt attttgaaca tactggggat acaaaattct ttgagcggtg tccgcaggtt
    28501 ctactgaatg cttcaagaat tattgaggac atattagctc aacgtcggtc aggggaacca
    28561 atgctttttc catctgtttg gttatcagac gcctttgcac ttggaaagta tcataccggc
    28621 agcaatcttt gtctttggaa ggcctgtatt ggacttgcag aaatttttga agccctagaa
    28681 caactgagtc ttagcaagcg atacaaaaac attgcctgta aactaaagga atcaatttgt
    28741 aaacaaatga ctatcgatgg tccttttggt gagcaatttt tggagggaat cggtgatgag
    28801 gagaaaacaa cctattcagt gaagcactat caaaaaccga ttcttgagca agggttaatc
    28861 tttttaagtg atgtgattgt ggatggaaaa attaatttat tgatgcatga tggagaagaa
    28921 tcagacacta cgttgatgcc tttttatcaa tttttagata ataaagatca tttatatcag
    28981 aacaccatgg ccttttctgc tagttctttc aatccaacct atagcgagga aatcaaaggc
    29041 atcacttggg gattagaatc cggcgccact tttcctggct ttatcactgt tttaatgagc
    29101 gagctgcaca accctcaagc tttcgtaacc cgattagaag aactaatttg tttggcagat
    29161 ttagacggtt cgtggtggtg gtggccgtat cgcttagagg ctcaacaagg agaagtcgtg
    29221 cgagactttg gctgcggcaa atgcggctgg gcatcgggtc tttttgtcag cctaatgatc
    29281 agtcaatatt tcggattgaa attcaaaaaa ggagtattgc aaattgatcc tgttgataat
    29341 ttaacgtttt cttggaagaa tttgaagttc ggtcaagcct tcatttcgat tgaatgtacg
    29401 caatccgagt tgagtatttc aaatttgtca gaaaagccat tgaaaataag tgatacgaaa
    29461 aaaattcttc acgtagaaaa gggaaaaacg attcacattc aaaggagaaa aagatgagta
    29521 tactaaaaaa tattacccaa tttaaaaaaa tccagagtcg agcaatcact cccgaaaatc
    29581 caacaggcga gaaaggtaaa gctgcgatgt tgccaagcga gctaggacca agccgcaaag
    29641 gtcgtggcgc aattccgctg acttctggtg aagaaactgt gattgcggat attactggaa
    29701 cgggaataat tcgtcatatg tggatgacga ttcgtgatcg aacagaattt ggcagttttg
    29761 ttttacgtga tttaatctta agaatttatt gggatgggtc ggatagccct gctgttgaaa
    29821 gtcctttagg ggatttcttt tgtaatgggt ttggagagcg ttgcgaggtg aactcgttgc
    29881 cgatagtggt taatcctaca gggggcttta attcctattt cgaaatgcct ttccaaacat
    29941 ccgctaaaat tacgatcgag aatttacatc cgaaggatat gtcctcattt ttttatacga
    30001 taaattacgc tttggtggac gaattggaag aaaatagcct atattttcac gcaaattggc
    30061 atcgggaacg cattaccaaa ttacaggagg attataagtt aattgatcga ttaaagggtc
    30121 aaggctatta cgtgggtact taccttgcgc ttacctgcct agaacgttat tggtggggcg
    30181 aaggcgagtt taaattctac attgatgggg atagtgagta tccgaccgta acctcaacag
    30241 gttcagaaga ttattttggc ggggcgtggg cctttcatga gtacgatgag ttcggacgtc
    30301 cacatgcgaa aaactattca acagcctttt tagggtatcc ttaccaatca aatagagatg
    30361 ccacaagaga tcgttttgaa acgggaaaat tgaatgccgt tcatgccttt ggtgacgacg
    30421 caatgccacg acatggtctt tatcgttggc atcttcaaga ccccatctgt tttaccgaag
    30481 atttatcggt cactttacaa caaatcggca acgatgatgt tcgcttattt gagcggcaag
    30541 acgatgttgc tagtgtcgct tactggtatt caacagaagc ttcaggaaat actaaacgtc
    30601 atttcactga tcgagaacgc ttgccgcgtt aatgaagtgt ttttcacata ttcgaaggga
    30661 ggaacctatg atgatatatg gggcaattga agctggaggg actaaatttg tctgtgcagt
    30721 cggtactgag gaattaacga ttttggaaag agtcagtttt cccacaacaa ctccagttga
    30781 aacaatgaag caggtcattg atttttttaa accttttaag gaacaattgg caggcatcgg
    30841 ggtcggctgc tttggtccaa ttgatattca atgcaaatca acgagtttcg gtcatatcac
    30901 atcaacacca aaacttgctt ggcaaaattt caattttgta ggaacgctaa agcaatattt
    30961 caatattccg attgaatgga caacagatgt caatgcggct tgctatggtg aatatgttgc
    31021 tggttctggc aaagggttag caggtgttgc atacttcact gtgggaacag gtatcggagg
    31081 tggtgccttg ttcaatggga ctttcgtgga agggttcagt catccggaaa tggggcatat
    31141 gttgatcaag cgacatccaa aggacaattt ttcaggaaac tgtcccttcc accaagattg
    31201 tttagagggg atggcagcag gaccagcgat tgaaaaacgg ttgggtgtaa aaggccagaa
    31261 tttattggct gacgattcgt tttggcagat tgaagccttt taccttgccc aatgtgcata
    31321 caatacaaca ctgatgtttt cgccagatag aattattttt ggcggtggtg taatgaaaca
    31381 agaacacatg aagaaaaaag ttcaggataa atttgttgag cttataaatg gatatgttga
    31441 aataccacca attgatagct acattattac gcccgaatta ggtgataatg ccggaataat
    31501 cggcggatta gcattggcca gaaaagcggt aagaaataag caaccataat taatgaatgt
    31561 ttcattgata ctcatgtcaa aagtatttaa ccgagtaatc aaatgatcaa atgaagttgg
    31621 ataaagcgaa aatgaattca aataaaaagg ctctaagtca aatcgtgttg ttttcattaa
    31681 atcactgaaa ggaaatgctt ctgcgtttcc ttttttcttt ttgttgtgcg ctgtatacct
    31741 tgaggatatt cactggtggc gtacacttaa atagtaatat attgtgttac tttatgtctg
    31801 tggtaatagt tatagttgct taccaatgta tgttcatttt tcgaaatgtt gacttcataa
    31861 tccttggctt aatgtttgaa tttttttggt gatagatttt ttcagccgta taaaataaat
    31921 cgtaattcta ttatagagag ttgtgattat aaaaagatag caagaaaaaa aaagaaagtt
    31981 tctatacatt ttaaatattt tattcctatt tctattgcta aaaaagtaga aattctatat
    32041 ttacctttaa ctctcgcttc tcttgcaaat gtagttagca ttttaatcga taataaactc
    32101 aggtagttat attaccttga aatgattgat aattttgtca cttctatttt ttttgcaata
    32161 aattttttat tttagtgaaa aataagaaac tatttctttg aaggcgataa gttagaaaaa
    32221 tattattgta gtcttttctt tattaggaat catcactgtt gtatcttatt accttttctg
    32281 tttttcttat tagagaaaaa taatttgaga ggtagatagt ttttattgga ggaagtaatt
    32341 atgtatattt ttgaaacaaa aatttttcat tgcaataatt gcaacaaaga aatttcatcg
    32401 ggggaagagg tttggacaaa aatggaaatt tccgccaaaa gtaactgctc ctcaactgaa
    32461 atcaattaaa gaactagaat ttgagaatgc actggtaatt tgtatgacat gtactgatga
    32521 agtatttcga aaacattttt aaaggttgag atgtatatag atttaaccaa tcatttatct
    32581 ctctcggaaa aggaatatta actaaccagc gggttatcaa aaataggtag gaaaactgta
    32641 cttaatggct tatttcgcgc tgattatgag aatatcagtc tgtttgatta atttttaggg
    32701 ttcaaaatac agagacaaac tccaggtcgg gtttcgccac aagcgaatta cccgatctgg
    32761 agttttgtct attgcaggaa ggcgtagccg gactgcagga ataaagcccc cggtt
//
LOCUS       DAFCNJ010000027        32419 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000027 DAFCNJ010000000
VERSION     DAFCNJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.028"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            191..1582
                     /locus_tag="LNE35_001538"
     CDS             191..1582
                     /locus_tag="LNE35_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBK6910701.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            2193..2561
                     /locus_tag="LNE35_001539"
     CDS             2193..2561
                     /locus_tag="LNE35_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBK6910702.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            2741..3682
                     /locus_tag="LNE35_001540"
     CDS             2741..3682
                     /locus_tag="LNE35_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBK6910703.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            3751..4482
                     /locus_tag="LNE35_001541"
     CDS             3751..4482
                     /locus_tag="LNE35_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBK6910704.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            4626..6806
                     /locus_tag="LNE35_001542"
     CDS             4626..6806
                     /locus_tag="LNE35_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBK6910705.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            6826..7308
                     /locus_tag="LNE35_001543"
     CDS             6826..7308
                     /locus_tag="LNE35_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBK6910706.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            7324..8085
                     /locus_tag="LNE35_001544"
     CDS             7324..8085
                     /locus_tag="LNE35_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK6910707.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            8216..9550
                     /locus_tag="LNE35_001545"
     CDS             8216..9550
                     /locus_tag="LNE35_001545"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBK6910708.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            9857..10342
                     /locus_tag="LNE35_001546"
     CDS             9857..10342
                     /locus_tag="LNE35_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBK6910709.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            10335..11018
                     /locus_tag="LNE35_001547"
     CDS             10335..11018
                     /locus_tag="LNE35_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK6910710.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            11144..13789
                     /gene="polA"
                     /locus_tag="LNE35_001548"
     CDS             11144..13789
                     /gene="polA"
                     /locus_tag="LNE35_001548"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBK6910711.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            13834..14670
                     /gene="mutM"
                     /locus_tag="LNE35_001549"
     CDS             13834..14670
                     /gene="mutM"
                     /locus_tag="LNE35_001549"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBK6910712.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            14634..15263
                     /locus_tag="LNE35_001550"
     CDS             14634..15263
                     /locus_tag="LNE35_001550"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBK6910713.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            15315..15647
                     /locus_tag="LNE35_001551"
     CDS             15315..15647
                     /locus_tag="LNE35_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK6910714.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            15959..16450
                     /gene="nrdR"
                     /locus_tag="LNE35_001552"
     CDS             15959..16450
                     /gene="nrdR"
                     /locus_tag="LNE35_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBK6910715.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            16473..17846
                     /locus_tag="LNE35_001553"
     CDS             16473..17846
                     /locus_tag="LNE35_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK6910716.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            17846..18772
                     /gene="dnaI"
                     /locus_tag="LNE35_001554"
     CDS             17846..18772
                     /gene="dnaI"
                     /locus_tag="LNE35_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBK6910717.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            18901..19341
                     /locus_tag="LNE35_001555"
     CDS             18901..19341
                     /locus_tag="LNE35_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK6910718.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            19485..20186
                     /locus_tag="LNE35_001556"
     CDS             19485..20186
                     /locus_tag="LNE35_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBK6910719.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            20183..21304
                     /locus_tag="LNE35_001557"
     CDS             20183..21304
                     /locus_tag="LNE35_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBK6910720.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            21319..22551
                     /gene="pepT"
                     /locus_tag="LNE35_001558"
     CDS             21319..22551
                     /gene="pepT"
                     /locus_tag="LNE35_001558"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBK6910721.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            22683..23591
                     /locus_tag="LNE35_001559"
     CDS             22683..23591
                     /locus_tag="LNE35_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBK6910722.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            23626..23898
                     /locus_tag="LNE35_001560"
     CDS             23626..23898
                     /locus_tag="LNE35_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBK6910723.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            23909..24955
                     /locus_tag="LNE35_001561"
     CDS             23909..24955
                     /locus_tag="LNE35_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBK6910724.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            25206..27815
                     /gene="clpB"
                     /locus_tag="LNE35_001562"
     CDS             25206..27815
                     /gene="clpB"
                     /locus_tag="LNE35_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBK6910725.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(27966..28652)
                     /locus_tag="LNE35_001563"
     CDS             complement(27966..28652)
                     /locus_tag="LNE35_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910726.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(28630..29124)
                     /locus_tag="LNE35_001564"
     CDS             complement(28630..29124)
                     /locus_tag="LNE35_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK6910727.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(29144..30364)
                     /locus_tag="LNE35_001565"
     CDS             complement(29144..30364)
                     /locus_tag="LNE35_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6910728.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            30511..32346
                     /gene="lepA"
                     /locus_tag="LNE35_001566"
     CDS             30511..32346
                     /gene="lepA"
                     /locus_tag="LNE35_001566"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBK6910729.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        10783 a         5759 c         6993 g         8884 t
ORIGIN      
        1 agattaaagc tatcacaggt atgttttttt attactaatt gtaagcaaaa agaatgtcat
       61 ttcgataaaa atcgtctaaa atataaaagt cactgaaaaa aattttttca ggaggatggt
      121 tgaagaatca ttctcgggaa cctttacttc cttcgatgga cttgtcatta ataatatgag
      181 gaggagaaaa atgacaacac tcatcacagt tttagtggtt ctagtttttg cggcattatt
      241 atatgtgttc tatcgtatgc aaaagcgcca tacaaaattt tcaacacgcg tctttacagc
      301 attaggagtt ggtatcgtct ttggcggtct gcttcaattg atctttggtg tagacgacaa
      361 agtcgttact caatcaatgg aatggatcgg gattgtaggt caaggatatg tatcattctt
      421 gcaaatgcta gttatgcctt tagtgtttat ttcgatcatt ggtgcattca caaaaatgaa
      481 agtttctgaa aagctaggga aaattagtgc gactgtattg acttctttac ttggaacaac
      541 agctattgca gcattgatcg gaattggttc aacaatgctg ttcggactcc aaggagctaa
      601 attcacacaa ggagctgcag aaacagcacg aatctctgaa ctggcaacac gtcaggaaac
      661 agttgaaaac ttatcgattc cacagcagat tttatccttt atccctaaaa acgtttttgc
      721 tgatttagca ggtagtcgtg caacaagtac gattggagtc gttattttcg cggcatttgt
      781 cggaatcgct tatctaggcg tgaaaagaaa agcgccaaaa gaagcagcat tcttttcaga
      841 tttagtcgac agtctgtata aaatcattat gcggatcgtg acgcttgttt tgcgtttgac
      901 accttatgga gtatttgctt tgatgacaaa cgttttggca acaagtgatt ttgcagctat
      961 cgtcaattta ggtaaatttg tcgttgcttc ttacgtagca ttgcttgctg ttttgattgt
     1021 ccactcactg attttgattg gtgcaaaagt caatccaatc aactatttca aaaaagcctt
     1081 tccggtattg agctttgcgt ttacttcacg ttcaagtgct ggagctttgc cattgaatat
     1141 cgagactcag acaaaagctt taggagtaga tgatgcaaca gctaatttct cagcaagttt
     1201 tggtttgtct attggacaaa acggctgtgc tggcgtttat ccagctatgt tggcaacgat
     1261 cgtcgcacca gcagctggag tgaatgtctt tagtttggaa tttattttga tgcttgtggc
     1321 aattgtaaca atcagttctt ttggtgtagc tggagtcggt gggggcgcaa catttgcttc
     1381 actgatcgtt ttaggggcaa tgaacttgcc agtagcaatc gttgggttag ttatctctgt
     1441 tgaaccatta atcgatatgg cacgtacagc agtaaacgtc agcggtagca tggtagctgg
     1501 tatcgtgaca agtgcacgca tcaaagattt agatcgcgat gtcatcaatg atgattctaa
     1561 agtgatcgat gtgaatgcat agtcaaaaag aggttgtgat agaagtggtc agctttaaga
     1621 aataagaaga agtttcctaa aaatagtttc tcaaattttt gtgaaatttc ggcttatttc
     1681 cgagaagcta cttctgaaac accgtgaata agaggttgtg aaaaagctgc cctgcatcaa
     1741 gtaataagaa cagccaaaca aaaatagttc ctcatatttt acgtttggtt gagcttatta
     1801 ccgcagatgc aagcttttga acaccgttta agaaagaggt cgtgagcctg ccgtttcact
     1861 ctgagtcaca aagagcaatt tcaaaaaata gcttctcatg tttgttgaat attgtgtctt
     1921 gtgaccgagg agttgtcagg agaacaccgt ttaccagagg ttgtgacttg tttttagtca
     1981 cgacctcttt ttttatttat tccatataaa tacacattat ttattccgaa aagtgaaagc
     2041 gcaaataaca aaaacaacca acttatttat tattaaagaa aagcgtattt aagtaggacg
     2101 attctgttga gagctttcgt agattttccc attttcctat ggtaaactga caaagttgag
     2161 aatgttagaa cagatcgttt aggaggaaac agatgtttac gaatgaaata aaaatcatgc
     2221 tttatgtgga cgatgtagaa aaaaatgcaa cgttttggag agcagttggc tttgcggaaa
     2281 aagatcgtca agaaatggat gggacgctta taattgaagt aggagttgag gacagtcaaa
     2341 cctcatttct gttatatgat cgagcatttg ttgagtccca ttcacctggc acagctacta
     2401 gtgccccatc tttgatgttt tacagtaaag atatctttgg actatataaa aaaatgcaga
     2461 cgcaatcagt cacattaggt gatatggtac agcttggaga agaatatgtc tttaactttg
     2521 ctgatcccga tggcaactac tttgcggtta caggtaaata atagaaataa agactttgtt
     2581 tttctgtgcg aaacaggaaa gcgaagtctt tttaattcaa ataataatat tatgtaaaca
     2641 aaaaactttc catgcgtaat tatacacaag caaataaatc gtcaaatgcg aacgccttct
     2701 ttatactgtt taggaacgaa aagaaggaag gaagaaatac atggattatc aagtactgct
     2761 ttattacaat tatacaaaaa tcgaagaacc agaggaattt gccaaaaatc atttagcctt
     2821 ttgcaaatct ttggatctta aaggtcgtat tctagtagct gaagaaggaa taaatggtac
     2881 tgtgtctgga accgttgaaa acacgactgc ctatatgaaa gcaatgaaag ctgatcctcg
     2941 atttgctgat acgatgttca agatcgatcc aagcgaagga catgccttta agaagatgtt
     3001 tgtaagatac cgtccagagc ttgtaagttt aaagctagaa gaggacattg atccaaacca
     3061 agtgacaggt gcctatcttt caccgcaaga atttaaagaa gctattttag atgaacagac
     3121 agtggtgatt gatgcacgga atgattatga atatgacttg ggacattttc gaggggctgt
     3181 acgcccagat atacgcagtt ttcgagagct tccagactgg atccgcgagc acaaagaaga
     3241 atttatggaa aaacgggtag tgacctactg tactggtggc attcgttgcg agaagttttc
     3301 tggttggctt gtccgtgaag gattcaaaga tgtcggacag ctgcatggcg gtattgctac
     3361 ttatggtaag gatcctgaag tccaaggaga attatgggac ggcaagatgt atgtgtttga
     3421 tgaacggatc gctgtcgata tcaaccaagt agataaacaa atcgtcggtc gcgactggtt
     3481 tgacggaaca ccatgtgaac gatatatcaa ttgtagcaat cctgagtgca atcgtcagat
     3541 tcttgcttcc attgaaaatg aagaaaaaca tttaggagca tgtagcatag agtgtgcgaa
     3601 acatccgcgc aatcgctatg ttcaagacca tggaatctct ccagaagaag cagaactgcg
     3661 gtttacagct ttaattaaat agaaaacaat agttttgatt gtcgaccatt tccaatatgc
     3721 gctatactag cgttgaggat ggtgatccac atgaaatcat tgatttatgc tattggggat
     3781 gtccatggca aacatgagct ttttgaaaaa cttattcact attatgatcc aaagatccat
     3841 cagcttgttt tattagggga tctcaatgat cgaggtcctc gtacaaaaga atgtttttta
     3901 ttaggaaagc aattagtcga agaaacagga gccatttacc tccggggaaa ccatgaagaa
     3961 tattttttgc aatttctgca atcaccggag gactggtatg cgccttatat gagaaatggt
     4021 ggaaaagaaa cgtttgaaag cctgattcac ccaggggcat cggaggaata ctccccaact
     4081 gaaatctcga tgatgatcca atcgagatat tcagaactga ttaaattttt agtccagcgc
     4141 cccttgtatt atgaatggga aaattatctt tttgtccatg ccggtgtgga tttgacaaag
     4201 aaagactggc gtaagacaag tctaagagac tttatttgga taagggaacc atttcatgaa
     4261 gggaaaaaca atacaggtaa gacaattgta tttggacata cgattacacc aatgctccat
     4321 ggagatatgc agacaacaga cctctggatt caggatcata aaatcggaat ggacggtggg
     4381 gctgtattcg gagggtcatt gcatggggtt gtttttgaca aggaaggtat tcttcaagat
     4441 attgaatatc agaacaacga aggaccttgg catggaatct gactaatgga aagaatgaag
     4501 gagctgtgac tttgttgcgg ctctttttat ttttatagag aatattgttt ctttcagtag
     4561 tttaacccag gataaaaagt atgatagaat aaagaagcaa tttttagcag aaaagaggca
     4621 ttttaatgac ggaaaatttg aaccagacga tcattcagct tgtacaaaaa gaactttcag
     4681 actatcgtcc taagcagttg actactgtac ttaatttgtt aaatgaagga aacactgttc
     4741 cttttatcgc gcgttaccga aaagaaatga ctggaagtct tgatgaagtc cagattcgtg
     4801 aaatcgaaga gcggtatgcc tatcttgaaa acttagaaaa acgtaaaact gaagtgattc
     4861 ggctaatcga tgaacaaggg aaactgactc ccgagttaga agcagaaatc acccaagccg
     4921 tgaaaatgca acaagtggaa gatttgtatc gtccatacaa acaaaaacga cggacaaaag
     4981 caacgattgc taaagaaaaa ggacttgaac cactcgcgaa gtggctgatg cagcttacag
     5041 atggagaggt gcaatctgaa gcagaaaaat atattgacaa agaaaaagaa gtctcttctg
     5101 ccgaagaagc gttgcacggt gctcatgaga ttattgctga acaagtcagc gataatgcaa
     5161 aatttcggac ttggattcgg tcttatacgt ataacaaagg tatgtatgtc agcaatgtca
     5221 aggatgagca agcagatgaa aaaggcgtat atgaaatgta ttatgacttt gcagaacccg
     5281 tccataaaat ggtttctcat cgaatattag ctactaatcg tggagaaaaa gaagatattc
     5341 tgaaagtatt ctttcaagta gatgaagcag caattttagc ttacttggat cgccaaatag
     5401 tcaaaaatcc tgcctctcct tcttcttcat tcgtcagaga agcctatcag gatagttata
     5461 aacgctttat tcagccagca atcgagcgag aactgcgcaa tgaattgact gaaaaggcag
     5521 atgagcaagc aatcgctatt ttcggcgaaa acctccgtaa tcttttgttg caaccgccac
     5581 taaaaggtaa agttgtcctg ggatttgatc cggcttatcg tactggatgt aaactagcag
     5641 tcgtagatgc gacagggaaa gttttagcaa tcgaagttat ttatcctcat aaacctgcag
     5701 cgcaagcaaa aagagaagct gctggaccag cttttatcca attaattaat aaatatcaag
     5761 tagatatggt agctatcgga aatggtacag ccagtcggga atctgaactt tttgtggctg
     5821 agcagttgaa atccgccgac cataaaacct attacgctat cgtcaacgaa gcaggcgctt
     5881 ctgtttactc cgctagcgaa attgcacgaa aagaatttcc gcatctacaa gttgaagaaa
     5941 gaagtgctgt ttctattgcc cgtcgcctgc aagatccttt agctgaatta gtcaaaatcg
     6001 atcctaaagc tgtcggagta ggccagtacc aacatgacgt ttctcaaaaa cgtttggctg
     6061 aacaattgga ttttgttgtg gaaacagcgg ttaaccaagt aggtgtggat gtgaatacag
     6121 caagcccgca gctcttgcag catatatcag gattgaataa aacaactgcc caaaacattg
     6181 tcatttatcg tgaagaaaac ggagaattca ccgcacgaac tcaattgaaa aaagtgccac
     6241 gcttaggacc aaaagcatat gaacaagcaa tcggattctt gcgagtccct ggtgggaaaa
     6301 acattttgga caacacaggt atccatcccg aaagttattc tattgcaaaa gaaatattaa
     6361 cttcagtcca actttcagaa aaagagcttg gtacagaaga agcagtagaa aaattgacac
     6421 gattatccgt agagaaactg gctgaaagcc tatcagtcgg agaagaaacg ttagccgata
     6481 ttttagctgg gctgacacaa cctggacgag atatgcgtga tgagatgcca gcgcccttat
     6541 tacgtacaga tgtattgagt atggaagact tgaaaccagg gatggaattg actggaacgg
     6601 tccgtaatgt cattgacttc ggcgcatttg ttgatatcgg tgtgaagcaa gacggacttg
     6661 tccatatatc gaaactcagc aaaaaattcg tcaagcatcc tacagatgtc gtgtcagtag
     6721 gagatatcgt aacagtttgg atagaacaag tcgatacgaa aaaaggcaga atcagtttga
     6781 cgatgctttc cccttatgaa gagtaacaat gaggagtaac aaaaattgac acaagaagaa
     6841 ctgcagcagt tagtagaaga gatatcatta aaaagcttcc agcttccttt caagcaccaa
     6901 gcaatgttca atccacgctt acgtacaact ggcgggcgct atcatttaaa cgatcatcat
     6961 ttagatttta atgcgaagct atttgaagag tcagacgaag agacgattgc aggaatcatc
     7021 aaacatgaac tttgccatta tcatcttcat ctagctggaa gagggtatca gcataaagat
     7081 atggatttca agtatctttt gaaaaaaaca ggagggttgc gctatgcacc agccttgacg
     7141 aagaaacaga taaagatgga gtattatcga tgccaagatt gttctgccac gatctgccgc
     7201 aagagaagaa tcgatgtacg caaatatcgt tgtggaagat gtcatggaga aatcaaatgg
     7261 attgagacaa aaagagaaga ctttaatgaa gcacaaaaaa tcagatgaaa ggatagaagg
     7321 cctttggaaa aaagtgagca gaggaatcta aaagactgga atcagctatt aaatttttta
     7381 gccgcacctg aagagggaaa ttttgaggaa ctgacggcac ctacacttat tttagctgga
     7441 aactgtcttc ctatcttagc tgataaagca gcacaaatgt atttaaatgg ccaagtagat
     7501 caattgtttt tggtcggagg ggtggggcat gcgactagaa tcctttatga gaattttgaa
     7561 aagcaaggat ttcattttga agatggcatg agtgaaagtg aaatctgtcg tcagtatcta
     7621 aaagaagtct atgatttgcc cgataaggct tttctgatag aatcgaagtc tacaaattct
     7681 ggagagaacg ccactttttc actggagatt cttcattcct taggagctgt accagaaaaa
     7741 atcttattga tgaatgatcc gacactgcaa cgcaggactc gagcaacttt tgagaaagta
     7801 tggcaaaatg aacaaactat tttcgcgaat gctgtgccat ttgtgccgga aatccttcac
     7861 ttttctgaag agatcatctt tacagcaaaa gaactgaatc atcaatggcc taaggaatat
     7921 ttccatgcgc tagtattagg agaaatggaa cgtctccatg acgatgagaa cggctatggt
     7981 ccgaaaggaa aagattttat tcctcacatt gatatatcag aggaagtttg gtcttcttac
     8041 accagaatta aagagtcaat aaaaacggat ttttctcgta cttgaccatt ttagcaagcg
     8101 aatcctcctt tccaaataaa tttggttgaa attttcaatc gtttataaga taatgaggag
     8161 cgtgttgaat gtaatagcta aaagaatgga cgctagataa gagaagagga ttcaaatgga
     8221 aaatcaaaat aaattgtatc attttgttgg tatcaaaggt tcaggaatga gctccttggc
     8281 tcttgtttta catgaacaag gtctaaatgt ccaaggatca gacattgaaa aatatttctt
     8341 tacacaaaga gatttagaaa aagcaaatat cacgattctg ccatttaacg cagataatgt
     8401 aaaaccaggg atgacgatca ttgcaggaaa tgcatttcct gattcacatg aagaaatcca
     8461 gcgagcaaaa gaattaggtt tggaagtgat tcgctatcac gactttattg gtcattttat
     8521 ccaaaattat acgagtatcg ctgtaacagg ctctcacgga aaaacaagta cgactggact
     8581 gctctctcat gtattgagtg gtgtccgtcc aacaagttat ctaattggag atggaacagg
     8641 acatggtgat ccgcaagcgg aattcttttc atttgaggcc tgtgaatatc gccgtcattt
     8701 ccttgcctat tcaccagact atgcaatcat gaccaatatc gattttgatc atccggatta
     8761 ttatacaagt atcgacgatg tatttacagc tttccaaaca atggctggac aagtgaaaaa
     8821 agctattttt gcgtatggtg acgatgctta tttacgcaaa ttaaaagcaa atgtgccgat
     8881 ttattattat ggtgtcacag aaaacgatga tatccaagca cgaaacatcg aacgcacaac
     8941 ttctggttct gcttttgatg tctatcatgg ggatgaattt gttgggcact tcactgtacc
     9001 agcattcggt aaacacaata tcttaaatgc tttaggtgtt attgcagtag cttatttcga
     9061 aaaactggat ctgaaagaag ttgcagaaga aatgctgact ttcccaggag taaaacgtcg
     9121 attcagtgaa aagatcgtag cagacatgac cgtagtcgat gattatgcgc atcaccctgc
     9181 agagatcaaa gcaacgattg atggcgcacg ccaaaaatat ccagataaag aaataattgc
     9241 agttttccaa ccccatacgt ttacgcgtac gattgcattg atggatgaat ttgcagaagc
     9301 acttgattta gctgataaag tttacttatg cgatattttt ggttcggctc gggaagaaca
     9361 aggaaacgtt aagattgaag accttggagc gaaaatcaaa aaaggtggag aagtcatcaa
     9421 agaaaataat gtatctccat tgttagatta tcatgatgca gtagtaatct tcatgggtgc
     9481 tggagatgtt caaaaatttg aacaagcgta tgaaaaactt ttaagcagca cgacgaaaaa
     9541 tgtactgtaa tacaattact gcagtgtcct tataaaaata gaaagagggg gagtggcaaa
     9601 aatcttgcca agcccccttt tttgcaactt atgataaacg gtgttcaaaa gcttgcatct
     9661 gcggtaataa gcccaatcaa atcaaaaaca tgagaagctg ttttttcttg gctgttctta
     9721 ttatttgatg caggacagct ttttcacgac ctctgtaaac tataggaatc tgttaaatca
     9781 agggagcaga acttgccttt tacgctactt ttggtacaat aatgtatgat taaaaataaa
     9841 ctggagggtt tctatcttga acataggtaa gccgagaaaa ctaggaaaaa caataaatga
     9901 aatcgaggaa ggcgactcgt tatccttaac agaatctatt gaagataacc agcttttgct
     9961 gtatttggga ttgacgaatg atgcaaatcc tttgtatatc caacatgact ttgcacaaaa
    10021 aacagaatat caacagccaa tcgttccatc agtgatgctg atggggatca tcacaagtgc
    10081 aatttcaaag catcttcctg gaccaggatc acatgttgtc aatttttctg tcaattttat
    10141 tgaaccggtt ttccattatg aaacactgac ttttcagttt gaggtcataa aagtagataa
    10201 gatgaaggat gtggtgacga tttctgtaga agcggagaat acgaacgaag atcgtgtgtt
    10261 ggatgctgtc gttatggtcc aaccgccaca agttcctgaa ttggaaatag caatcgaaag
    10321 tgaaggtgaa ttgaatgaat aatccaagta catcaatgtc aggtatcaat cgtttttatg
    10381 caaaagttta tggattttta ggtatgggac ttgcccttag tgcagtcact tcttacttgg
    10441 ctctttgggt atttcctttg caattagcaa cttttatcaa taattttcca ctaggctttg
    10501 tgggtctgtg gatcgttgaa ctgattttag taatggttct aggcgtaaaa gcacaaaaga
    10561 atccgagctt agcaatcggt ggatttattg cctactctct tttgaatggc ataatattag
    10621 cggttactct tgcatactac gatattggaa cagtaacgca agctttcttg acagctactt
    10681 tgatgttcat ggggatgtct gttgtcggag taacaacaaa aagagatctt agcggggttg
    10741 gaagagcagg acaggcagct ttaatcggag tgatcattgc actagtcttg aacatcttca
    10801 ttttgagaag tagcgtagtt gagctgttca tctccctact gttagtcgtc atctttgcag
    10861 ggattacagc ttatgataac caaagcatcc gtaatatcta tgtccagaca aacggaacag
    10921 cacatagtgg aattgctgta ttcatggcat tgaagctgta tttggacttt atcaacttgt
    10981 tcttggcgtt tctgagaatc tttggacgga acaactaaaa aagaaaaaaa ttcatcagtt
    11041 ggggctatac cgaattattt ggtatggtcc cgctttttct tttcgcattc cgcaggattc
    11101 tttgttagaa tgaataagaa ctttacagta aggagttaag aagatgacaa aaaataaatt
    11161 attactcgtt gatgggaaca gtgtggcttt tcgcgcattc tttgcgcttc acaattctct
    11221 tgaacgtttc aaaaatagaa acggtcttca tacaaatgca atctatgcat tcaatacgat
    11281 gtttgaaaat gttatgcaaa aagagcaacc gactcatgtt ctggttgcgt tcgatgcagg
    11341 aaaaacgact tttcggacag aaatgtatgc ggagtacaaa ggtggaagat ccaaaacacc
    11401 tggcgaattc aaagaacaaa tgccatatat acgtgatttg ttgacaggac taggggtaca
    11461 gtattacgaa ttgcccaatt atgaagcaga tgatattatc ggtactttgg cagaaaaagt
    11521 agacaaagat caatttgatg tcgtcgtgct ttcaggtgat cgggatttga ctcaattagc
    11581 tagtaaagaa gtaaaagtgg atatcactgt aaaaggtgtc agcgatatcg aaagctatac
    11641 accagaacat gtagcggaaa aatatgatgg gttgacgcca aaacagatta ttgacatgaa
    11701 gggactagcc ggagatgcct ctgacaatat ccccggtgtg actaaaatcg gtgaaaaaac
    11761 agctatcaag ctattgaaac aatatggctc agtcgaagga atttatgaac atatcgatga
    11821 aatgaagcaa agcaaaatga aagaaaatct gataaatgac aaagagcagg ctttcttatc
    11881 gaaaaaattg gcgaccatca atacttcagc gcctgtggat gtctctatcg actcattgaa
    11941 gtatgaaggg aaaaatctcg ataaactcgt tccgttttat aaagaaatgg atttcaatca
    12001 gttcctttct aaattgaata tcgtagaaga agaagtaaaa atggatgata ttttgtttga
    12061 agtcgttcat gaattcaaag aggaaatgtt tactacggat atggcgttgt acgtggaaat
    12121 gatgggcgac aattatcata cagaggaaat cgtcggagta gcgtggggga cagaaaagaa
    12181 aatttatgtc acgaacgatc tatctgtatt cgaaaaccga gcgttccatt cttggatcac
    12241 tgatccgact cggctgaaaa aagtatatga tgcgaaacga acttatgttg ctttaaaccg
    12301 ctatactgga aaaacgaaag gaatcgattt tgatgtcttg ctaggtgcat accttctcga
    12361 tacgaatgaa aaaagcacag atatcgaagg aatagctgct cattacggat acaacgatat
    12421 ccaatcagat gaagctgtat acggcaaagg ggcgaaaaaa ggtttaccag aagaagagga
    12481 actctttttt gctcatttag cgcgaaaagt agctgcaatc aatgcgttga caccaaaatt
    12541 gagccaagaa ctggcagaaa aaaaccaaga agatcttttc cggaaaatgg aactgccatt
    12601 gtctatcatt ttggcagaga tggaaataag cgggatcaaa gtagatgcga ctcgcttaca
    12661 agaaatgaag ggcgagttct ctgctcgctt aagagagatc gaacaaaaaa tctacgaaga
    12721 agccggcgaa gaattcaatt tgaattcacc aaagcaatta ggagtgatct tatttgaaaa
    12781 aatgggatta cccgttatca aaaaaacaaa aactggatat tccacagccg tagatgtact
    12841 ggaacagctt cgtgaacaag caccaatcgt agaggatatc ctcacctatc gtcaaattgc
    12901 aaaaattcaa tctacctatg tcgagggact gctgaaagtg atccaatcag atggaaaagt
    12961 gcatacccgt tatgtccaga cattgaccca aacaggacga ttaagctctg ttgatcctaa
    13021 tttgcagaac atcccgattc gattagatga agggagaaag atacgtcagg catttgttcc
    13081 tagagaaaaa gactggctga tttattcttc tgactattca caaatcgagt tacgcgtact
    13141 tgcgcatatc tcaaatgatg aacatttgaa agctgccttc ctagaaggtc aagatatcca
    13201 tgccagcaca gcgatgcgtg tatttggaat cgaaaaggca gaggatgtca caccaaatat
    13261 gcgtaggcag gcaaaagcag tgaattttgg tatcgtgtat ggcatctctg attacggact
    13321 ttcacaaaat ctaggaatca gccgaaaagc agcacagcaa tacatcgata cgtactttga
    13381 aaaataccca ggtgtcaaag aatatatgga aagaatcgta cgcgaagcaa aagaccaagg
    13441 ctacgtcgaa acgctgtatc accgccgtcg atatctgcca gacatcaatt caagaaatta
    13501 caatatccgt tcttttgcgg aacgaacagc tatcaataca ccaatccaag gaagtgctgc
    13561 tgatattttg aaaatcgcga tgatcgaatt ggataaacga ttaaaagaaa caggtcttca
    13621 agctacgatg ctgttacaag ttcatgatga acttgtcttc gaggtaccgg aaaaagaact
    13681 tgaatcatta gataaattgg tgaaggaagt tatggaacaa gctgtttctc ttcatgtacc
    13741 actgatcact gacagcagtt ggggaaaaac gtggtacgaa gcaaaataaa cattgtttaa
    13801 gacagagcaa tataatgaag gtgaaaagaa aaaatgccag aattaccaga agtagaaacc
    13861 gttcgtaaag gattggaacg gttagtagta ggaaaaacga ttcaaaaagt tcaggtcttg
    13921 tggcccaaga tcatcgagca accagagaca cccatctttg aggcttctct tgttggcgaa
    13981 accatccaat ccattggacg tagagggaag tttttaattt ttcatctcga tcattgtgaa
    14041 ctgatctcac acttgcgaat ggaaggaaaa tatcaattta cgaaagaaaa tacaccaatc
    14101 gataaacata cgcatgtact tttctttttt gaggatggaa gccaacttcg ctacaacgat
    14161 gtacgaaaat ttggtcgtat gacaatcgtc gaaaaaggag caagtgcaac ttaccgagga
    14221 atcatgaaat tgggacctga gcctttgcca gatagcttct tattagcaga tttcgcaaat
    14281 ggactgaaaa agtcccacaa agcaatcaag ccattgctgc tagaccaacg tctagtgact
    14341 ggtctaggta atatttatgt agatgaagca ttatgggaag cgaagatcca tccagaacaa
    14401 ccggcgaaca cactgcgccc caaagaggta gaacaattac gtctatcgat cattgatgtt
    14461 ttggatcgtg caatagaagc cggaggtacc acgattcgca gttatttgaa tgcattagga
    14521 gaatcaggcg gatttcaagt agcactgcat gtctatcagc aaactggcaa accatgtata
    14581 cgttgtggaa cgccgatcgt gaaaacaaaa gtggcacaaa gggggacgca ctattgtccg
    14641 aattgtcaaa ggctaaaaat cgcaaagtag gatttgtttt aggtttgacg ggtggaatag
    14701 ctaccggtaa aagtacggca gccaaagtct ttcagtctca tggatttcct ttgatcgatg
    14761 gcgatgtgat tgccagagaa actgttgagc ctggaacgct tgctctgagg aaaattgtat
    14821 cagtgtttgg ccaagaggta ttgcagcctg atgggcaact agatcgaggg aaacttggga
    14881 tgattgtttt tccatcaaag gaaaaacggc aaaaacttga tcagctatta gatccgttta
    14941 tccgaaaagc gataaaagat caaatcgcct ctctaagttc caatcatcct ttagtaattg
    15001 tagatatccc attgctctac gaaggtcatt atgaccacta tatggatgca gtagctgtcg
    15061 tttatactac gccagaaact cagcttcagc gtttgatgaa acgtaatcaa ctgacgctag
    15121 aacaagcgca gcagagaatt agtagtcaac ttccaatcga agaaaaaaag cagcgtgcgg
    15181 atattttgtt cgacaacaat ggaacaaaag aaatacttgt gggacaaatc gaaaattggc
    15241 tttttgctaa tcgctttctt tgagtatctg tgtcaatttt gctatcctga gtatatagaa
    15301 agtgagggat agcgatggga tcattaaaaa atcaaaagga taaagtcgta ggtaacgcaa
    15361 aagaaaaaat cggcgagatg ctcaataaag aaaatctaat cgatgaaggt cgtacgcaat
    15421 cagaaacagc agaagaaaaa gaaaagcagt accaagcaag aatcgatgaa atagaagacc
    15481 agcaggcaga agaagaagcg aagcggcaag atttgactca agaacgaaaa aatccgacac
    15541 ctgatcgtag taaaacaccg ctcccagaaa gtgaccacat tcccgatgag caaaaaatcg
    15601 aggaacagcg aatgaaacac tatacaggaa tcgaagagaa gttataaaat agtaaaaaat
    15661 cgagatgtga cttgaagcag atgagaaggt gttcatctgc ttcaagtttt ttgtgctaga
    15721 tgactaaatc agcttttgtg taagttattt ctcagtaatc aaggtgatca gtattggaaa
    15781 attgaaattt agtaaggaca ggggacacaa ttacgcatga aagtagaaaa aattgttctt
    15841 atatccgtgt tttttgggta ggacagtcgc gtttgtgatt tgtttcgtgt tataatacaa
    15901 ctagttgtaa agaaagacac atcctttata taaaatgcga tgcaagaggt gagacaaaat
    15961 gagatgtccc agatgtcaac ataataattc acgagtaatc gacagccgtc aagcagatga
    16021 tggacgtgcg atccgtcgcc gtcgcgaatg tgagaattgt tcgttccgat tcactacatt
    16081 tgaacgaatt gaagctgctc cgttattagt aatcaagaaa aacggggaac gcgaagagtt
    16141 caatcgcgat aaaattttaa gaggactcat ccgttcagca gaaaaaagac ctgtagctat
    16201 ggaacaaatg gaacaaatcg tcaaccatgt cgaaaaccgc gtacgcagtc tcggagaaaa
    16261 cgaagtatcg acgaatctaa ttggtgaata tgtgatggaa gatttagttg atttagacga
    16321 aatcgcttat attcgttttg caagtgttta tcgccagttc aaagatatgt ctgttttctt
    16381 aaaagagttg caagatatcg tggataaagc aaaagaaaat aataaagaga ataataaaga
    16441 aaacaattaa gaaacaaaaa ggaggcattt ccttggaaaa tgcttggaaa gagcttcaac
    16501 caaaaaatat ttaccaagga agaaaagcgg ctccgctgag agaagacagc aagaacgcct
    16561 tgctgtatct ttatcagcca ctaatcggcg gagaagcctt gagtatctat ttcaccctgt
    16621 tatcagaaat ctctcaagaa tctggcaaag gtcctgaagg gatgcatgca gatctgctta
    16681 gtagtctagg ctgcggtatc ccgcaatttt atgatgcaag aaaaaagcta gaaggaatcg
    16741 gcttactgga tgtttatttt aaagaagata gtgatctggg acctcgtttt atttatgagc
    16801 tgttagaacc actcattcct aaagcatttt tcaaagactc tgttctggct tttcttcttc
    16861 tggaaaaagt tggtgaacga cgattccaga agcttaccca attctttgaa cctaaaaggc
    16921 atacagcaga gggatatcaa aaaatcacaa aaaaattttt agatgtttat cgcttcaatg
    16981 aagcagtgta cgctgctaat caagaaaaca tcgaatcgct gaataaatct tttgaagaat
    17041 ctgaatcagt agcattactt gaagcagatt cgacattcga ttggcagttt ttgaatgatt
    17101 ggctggcaaa aaaacatatt gcaagtcttg atgatccaat catcaaacaa gtcgacatgt
    17161 atcatcagct ttacggtttc gatgaattga cacttggcga actgatcagt caatcttatg
    17221 actttagtga acaaaaagta tctgaaaaag aactgcaaaa agccattttg gcaatgcaga
    17281 gacgtccggt gaagcaaaca aaagaaacag ttgcggtttc tgaaaacagt aatgaaccct
    17341 cgtcaaacga acatttgaca aacgcagcgc ttcagctgat aaaagaagca cagagcattc
    17401 caccgatgaa atatctggaa gcaatcaagt cagaaaaagg cggatatgtt tctaaacaag
    17461 aaaactggtt actgcaagat cttgtttctc aatcaggtct ttccagtagc gtgatcaatg
    17521 ttttgctgaa ttatgtacta gtgatcaaga ataatgctag cttaaatgcc agttatgtaa
    17581 atacagtggc aaatgaatgg gcacaaaaga aaatcgccac agcagaagaa gcgatccagc
    17641 acattcggca aataagtaat caagcaaaac aatcaaaaca aaagaagcaa acaaattatc
    17701 aaactggcaa gcgaaatgtt cgacgggaaa agcttcctga ttgggtcaac cagccaaaag
    17761 atgaaaaaca gatcagcagc gagaaaaaag cagagattga ccgccgtttt aaagaatacc
    17821 tggcaaaaaa ggagggtgat atctagtgga agacgtaggc aaagaattga agaggatcat
    17881 cagcaaacgc aattatcaag aaaagtatgc tgaaatgatc gctgaagtct taaatgatgc
    17941 ggatgttcaa gcttttctaa gcgcacacca agaagaattg acacaagcgg atattgaaaa
    18001 aagttacgct aaattgtatg aattcgtgca agaaaagcgt aaatatcaaa aaaacgatcc
    18061 aacgatgatc gctcccggct atgagccgaa gctgacgttg aattttcatt atgtggatgt
    18121 cacttatgta ccaacggaag cattgttagc acggcaaaga caagaagaaa tcagaagccg
    18181 agtaaaagca atggatatgc caaaagatat tcaatcggct tcttttgaaa actttgaacg
    18241 tacagatgga cgaggatatg cgagcttaga agccctagat tttgtagaga aatatactgc
    18301 tgatccaaaa gggtatcata aaggactgta tctagtagga agttttggta ttggaaaaac
    18361 atatttatta ggagcaacag cgagagaatt agcgattgct ggattcacca cgacgctagt
    18421 ccattttccg acatttgccg ttgagatgaa acaggcaatc ggaaaagatc aagtggcaga
    18481 aaagttagat gcagtgaaac gttcacctat tttgatgatc gatgacattg gtgcagatgc
    18541 gatgagtagt tggatacgag acgaagtatt cggtgttatc cttcaatatc ggatgcagga
    18601 acagttacca accttcttct catcgaattt tacaatggat gaattggaac agcatttgtc
    18661 cgtcactcaa cgtggagatg aagagccgct aaaagcgaaa cggataatgg aacgcatacg
    18721 ttatttgaca aaagaaatcg aaatgactgg tcgcaaccga cgcaatggtt gaacagttga
    18781 caagtccaac tgacatttga cctagtagta aaagcaggtc agcgaatcat tatatggaca
    18841 gaaatgaccg gagacaatca ccgcaacaag taatgcagaa acttaaacgg aggatttttt
    18901 atgagggcta ttttttctag tatatctgta gttggtctat tttttctttt agcaggctgc
    18961 ggtagcgcag atagcaacac aggttctgtt gattcgattg atgcgggtca aacagtaaaa
    19021 acaagcagta cgaaaacgga gagacaaagc atatttgtcg gaacgattga aagtatcact
    19081 caagtagaag cagaagaaga aacgcttcag ttgaaactcg tcgatgtcat ggccaaagaa
    19141 gatccagcga atatgggaac gtcattcagc aatgacggag tgatcttaaa tgctactgct
    19201 gaaaaaataa gcgtggacat aaatgaattg aaaatcggtg acaaagtcca atttacatta
    19261 gttgagatgc cgatcatgac gatgtctatc ccaccgcaag ttcctggaga ttcgattatt
    19321 gatgtttcat tgaacaaata accaataaca ttttttcaaa aataagaaaa tgttgtttta
    19381 tagaacgaaa aggtatattt tttaaggaga tatgggaatt tttttattcc tagtctcctt
    19441 attttatttg gaaaatgcta taattatcta gaaggagtga agctatgaac catttagatt
    19501 tatctcagcg cttagccgca gtgggtgact ttgtccctga atccgctcgt ttagcagaca
    19561 ttggatcaga tcacgcttac ttacccgttg cattgatatt aagaggaaaa atcgaatatg
    19621 cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc acgtaagcaa gtcaaaaaag
    19681 atggattaga agaccgtatc accgtacgcc ttgcaaatgg tctagatgct atccagccac
    19741 aagatggtat tacagctgtc acgattgcgg gtatgggagg ttcactgatc cgagatatcc
    19801 tagaagctgg caagttgaat caacgcttaa gcggacaaga acgtttgatc cttcagccaa
    19861 acatcggaga gcgtacatta cgatcttggc tgcaagaaaa tcagtataaa atccaatcag
    19921 aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat tgtagccgaa aaagaacaaa
    19981 aaccagttac ttacaatgaa atggagctga tgttcgggcc atttctgctt gaagagaaaa
    20041 gtgagatctt caaatcaaaa tggacaagag agcttcaaca aagaaaagct atccgtgaac
    20101 agctcaaaca agcagctaat caagaggcac gtcaaaaaca aatcgagcag gaaatccaaa
    20161 tgattgagga ggttttaaag ccatgacaat cagcggacgc actttcatcc aaaagtttga
    20221 aagctattgt cctcagtggc tagctgaaga aggagaccca gtcggactgc atatcggaac
    20281 attggataag ccaatccaac gagtaatgat gacattagac gttcgtccag aagtggtcga
    20341 agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa catcctccaa tctttcgtcc
    20401 tgtaaaacgg ctggttactg atcagccaca agaaaaaatg tatgcagatt tgttgaaaca
    20461 tgatattgcc gtctatgcag cacacacgaa catggatatt atttgggatg gactgaatga
    20521 ctggttttgt gaacttctcg gtattgaagt ggagagttat ttagtaaaga ctcatgagat
    20581 tcattataaa aaactggccg tttatgtacc tgtggatcat gcacagaaaa tgcgtgaagt
    20641 attagctgca gctggtgctg gtacacaagg tgattatacc ggtacaagtt ttacctcgat
    20701 tggacacggt cgatttactc cggaagctgg tgcacaacca gcgattggga aagtagggaa
    20761 gacagaacaa gttcaagagg caaaagttga ggttattttg ccggaaacta tcgaaaaaca
    20821 agtgattcaa gccatgcggt cagctcatcc ttatgaggaa cctgcttatg atcttttcgc
    20881 tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta ggtgaattgc cgcaagaaat
    20941 ttctatagag gcatttgtag aacaagtaaa agaagcgttc caactagatg gactgagaat
    21001 agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt gcgatttgcg gtggaagcgg
    21061 tgaaaaattt tatccgcaag caatcgccca aagagcagat gtctatatca cgggagatat
    21121 ctattatcat acggctcatg atatgcaaag cgctgggttg attgcaattg atcccggtca
    21181 ttatattgaa tcgttatgca agcaacgatt tatcgagaaa tttgaatctt ggaaacaaga
    21241 agaaaattgg gatattaact ttttcgtttc tgaaacaaat acaaacccat ttcaatttaa
    21301 ttaaaaggag agaatactat gtacgaaaat cttttgccac gttttttacg atatgtcaaa
    21361 acagaaacac gctccgatgc aacaagcaaa acgactccat cgactcagac acaagttgct
    21421 tttgcgcaag aattgaaaaa agaattagaa gagttaggct tagctgatgt tttctataat
    21481 gaagataacg gattcgttat gggaacatta ccaagcaatg ttgatcatga agtgcgttcc
    21541 attggattta tttcccacat ggatacagca gattttaatg cagtaggagt caatccacaa
    21601 atcattgaaa actatgatgg cgagtctacg atcgtactag atgcggaaga acgctataca
    21661 ttgaatacaa aagattttcc aaatttgaaa aattatgaag gccacacatt gatcacaaca
    21721 gatggcagta cattactggg agcagatgac aaatcaggga ttgcggaaat catgactgca
    21781 atggagatct tgatcaataa tccttctatc cctcacggta ccatcaaagt ggcttttggt
    21841 cctgatgaag aaatcggtgt cggagcggat aaattcgatg ttgagcaatt cgatgtagat
    21901 tttgcttata cagtcgatgg tggtccttta ggtgagttgc agtatgaaac gtttagtgct
    21961 gctcaagcaa atattacgat ccaagggaaa aatgtacatc cagggacggc aaaagataca
    22021 atgatcaatg ctctacagtt agcaatcgat tttcataatc agcttccagc cgatgaagtt
    22081 ccagaaaaaa cggaaggata cgaaggtttc ttccatttga tggcattgga tgggaatcct
    22141 gaagaagctt ctatgagtta tatcattcgc gatcatgatc gtgaaaaatt cgaagcacgc
    22201 aaagcacaaa tcacacatat ccaagaagag ttgaatcagc gatttgatca agaacgaatc
    22261 aaagtagata tgtatgatca atattacaac atgaaagaaa tcattgaaaa agatatgagc
    22321 atcgtggaat tagcgaaaca agcaatgatc gagcttggta tctctccagt gatcgaacca
    22381 gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg gtattccgac gccaaatcta
    22441 tttgccggag gagaaaatat gcacggacga ttcgaatttg tttctttaca agtaatggaa
    22501 aaagcaacag acgtgattgt aaaaattgca gaattgaaca caaagagtta aaatcgaaaa
    22561 agaacagctt atgacaaggc gttcgtccca atgatggaat gtcatctgtc taaggctaaa
    22621 gggcaggaca atggaattgg ttgtcccgcc ctcttttttg aagagtaaat aggaggtaaa
    22681 gaatggaaaa gctgctagtt ttttataaca aaagttcagg gaaagatgag ggtgagaaat
    22741 tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc agaattatat gtagaactag
    22801 ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa gaaagcagaa gccatagaag
    22861 ctgatacttt agtaattata ggtggagacg gcacgattca tcatatcgtc caagcgttcc
    22921 aaaaaacatt atctcaatac catgtcggat tacttccagg tggaactgtc aacaaccttg
    22981 cacgtgttct tgagattccc ctcaaaaaag aagaagctgc tgacgtgatc ctggaaggca
    23041 atgtacggaa aatagactat ggtcaagtca atgatgaagt cattatctct acactgacaa
    23101 tcggaatctt agcagataca gcagtttgga tcagtcaaga agaaaaacag cgttacggct
    23161 cgtggatctt tatcaagaag ttttttaagt tattgctgaa gaagaaaaaa tatcatttgg
    23221 atttagaaac agacgaggag cattggaaag gaaaatccca actgttgaca gtcacgatgt
    23281 ctaactccgt gggcggtttt acaaattttg acgactcagc gaccctagat gatggaaaat
    23341 ttcatgtaac gatcgtacct tccttagata ttttccgatt tattttttat ttgcctagaa
    23401 tcatgagagg caaattctat acgatccccg gcattactta tttcacagcc aaacgcatga
    23461 aaatccaagc gcaggaaaaa tctgtgggca ctcgaacgga tggagacacg acagatgatc
    23521 ttccagttga attatctgtt gtatcaaaag gattaacagt ttattcacca aagaaagaag
    23581 aaaaagctta gaaatagtag ttaacagaaa gttggaagaa gagtaatgga tgtattgtta
    23641 cacaatcctt tgtggtttta ccttttcatt gtgaattttt acttattttg tctgatgggg
    23701 tatgataaac atcaggctag aactagcggc tggcgtgttc ctgagaataa tttgatattt
    23761 gtcagcctgt taggcggtgg tttgggagga ttgatctctc gaaaagtttt ccatcataaa
    23821 actcggaaaa agaagtttac tataggattc gctgcaggtg tacttgttga tttgattctc
    23881 atttattttt tccattaagg agcaggagat gtcgtctaaa ggaacgaaaa ctaaaatcat
    23941 tctgaatctg cttttgctga ttgtgatttt tgccattatt tattatttaa tcaatcaatc
    24001 atttgcagat atctttgccg aattgctgtc taccagtgtt gttgttttgg cagcggctgt
    24061 tatattaggg acagtttacc aaatcgctga agggcgctca atcaaagaaa ttgctgaacc
    24121 tttcgcgccg aattttacaa cgaaagatgg gttcttgacc tctagctata ttgcgtttta
    24181 ccgaatcgtt tcgtttggta caggaacgct gatctcggag atttattttt ataacaaaaa
    24241 aggaatgaag ctatcccagg gagcaggggt gacagcactc catatgatta tgtacaaact
    24301 ggcagtgatc acttatgcat tagctggctt ggtcatccaa ttctctcttt tttattctaa
    24361 tggtccatca atgatctggt ttatcatcgc tggtatcctt ttgacgatcg tgattgttgc
    24421 ctttttattg gcgatgtccg tcagcttgaa tgttcaaata ttttttgtca gcttatcgaa
    24481 taagttattt aaaagtgagc gtttgcggaa atgggtggat acatgcaatc agcagatcta
    24541 ctctttaagg gaaacggtcc aaaccattat ccaagacagg actgcgctat tgcggattta
    24601 tctatggaat atgttaaaac ttgtattttg gtatgtactt ccttatttga ttctgattga
    24661 aaaccatcct aaaatagatt ttctactgac catttcgttt atcagctttg cagtagtgct
    24721 ttcaggggtt attcctacgc ctgctggtat tggttccttt gaatttgtct atttgttatt
    24781 attcagacca gttgttggaa cagttgacgc agtttcttcg cttttactgt atcgtttcag
    24841 ttcctttatc ttgccatttc tctatggatt tgtgtatgtg attgctgaac ggcgaagagt
    24901 gatcaaagaa gaaataatag aagtaaaaaa agagaataaa gaaaatcaag ggtaggtctt
    24961 ttataagatc tacccttttt atcaaatcaa agaaaaacgt tatggataag ataattttac
    25021 gtttgtttgg ctcttttttt atatacatac aatcaatttt gtttttttaa gatttatata
    25081 agtcgagaaa aaaagtcaaa aaactatgtg tatattttga ccaaaattga ccaaagcgat
    25141 ataatagttt ttgtaaagat caaagaaata agtgatctta cctttaatta ggagggtata
    25201 cctgtatgaa tattgagaaa atgactacga cgcttcaaga agcgattgct gaagctcaac
    25261 agattgcggt cactcgaaaa catcaagata tcgacattgc ccatgtatgg aaaatctttt
    25321 tacaatctaa tcattttggt cgaaatttct atacggatgc cggattggat gtcgaatctt
    25381 ttgaacatga aattgatcgt ctactggatg agtatccagt tgtttctggt ggaaacgtcc
    25441 aatatggaca aaacctcagc cagaatttct ttcgattatt gaatgaagca gaccaaatac
    25501 gtgaaagctt cggcgacgag tttctttcaa cggaagtcgt tattttagcg ttgatgaaat
    25561 taaaaaatta tccgttaact gtttatttga ataaaaacgg attatctgaa aaagaattgc
    25621 gtaaaaacat cgaagagatg agaggaggag aaagagtgac ctcacaaaac caagaagaac
    25681 aatacaaagc attagaaaaa tacggcgttg atttagtaca gcaagtcaaa agcggaaaaa
    25741 tggatccaat catcggtcgt gatgaagaaa tccgtgatgt catccgtatt ctttcacgaa
    25801 aaacaaaaaa taatcctgtt ctgataggag aacccggtgt agggaaaacg gcaatcgttg
    25861 aagggcttgc tcaacgtatc gtacgcaaag atgtgcctga aaatttaaaa gataaaacga
    25921 ttttttcatt agatatgggt gcattgatcg ctggagcaaa attccgtggt gaatttgaag
    25981 aaagactaaa agcagtcttg aaagaagtca aaaaaagtga tggacgtatc ctcttgttca
    26041 tcgatgaaat ccacaatatc gtaggggcag gaaaaacaga aggaagtatg gatgccggaa
    26101 atctgttgaa accaatgctt gctcgcggtg aattacattt gatcggtgcc acaactttag
    26161 atgagtaccg tcaatatatg gaaaaagaca aagcgttgga acgtcgcttt caaaaggtat
    26221 tagtcaaaga accaacagtg gaagatacga tcagtattct gcgtggatta aaagaacgtt
    26281 ttgagatcca ccatggtgtt aacatccatg acaacgcatt agtagcagca gctactttat
    26341 ctgaccgtta tattactgat cggttcttac cggacaaagc aatcgatttg atcgatgaag
    26401 ccagtgcaac gattcgggta gaaatgaact ctatgccaac ggaattagat caagttacta
    26461 gacgtttgat gcagttggaa atcgaagaag ctgcactgaa aaaagaatca gatgatgcaa
    26521 gtaaaaaacg cttgaaaaat ttgcaagaag aattagctga acttcgtgaa gaagcaaatg
    26581 cgatgaagat gcagtgggaa acagaaaaag aagaagtcaa ttctgtttca gcaaaacgtg
    26641 ctgaaatcga taaagcaaaa cacgaattag aagatgcaga aaataactat gatttagaac
    26701 gcgctgctgt attgcgtcat ggtacgatac ctcaattaga aaaagaattg aaggaattag
    26761 aagctaaagc aaaagacagc gagattaaga tggttcaaga gtccgttacc gaaaatgaga
    26821 ttgctcaagt cgttggcaga ttaacgggta tccccgtaac gaaacttgta gaaggtgaac
    26881 gagaaaaact aattaaattg aatgaaacac ttcataaacg agtgattggt caagatgaag
    26941 cagttgatgc ggtcagtgac gcggtaattc gttcacgggc tggattgcaa gacccaaatc
    27001 gtcctctagg ttctttcctt ttcctaggac caactggtgt cggaaaaacc gaattagcga
    27061 aagcgttagc tgagaacttg tttgactcag aagatcatat ggtacggatc gatatgagtg
    27121 aatatatgga aaaacatgct gtgtctcgtc tagtcggagc tcctccaggc tatgtaggtt
    27181 acgaagaagg tggtcaatta acggaagctg tcagacgaaa tccttataca atcgtcttgt
    27241 tggatgaaat cgaaaaagcg catcccgatg tcttcaatat cttgttacaa gtcctagatg
    27301 acggacgctt gactgattca aaagggcgag tagtagattt caaaaatacg gttctgatca
    27361 tgaccagcaa tatcggttca cagttactgc tggaaggcgt gactgctgat ggaacgattc
    27421 cagaagcagt ggctgaacaa gtcaacacgc tattacgtgg aaacttcaaa ccagaattct
    27481 tgaaccgaat cgacgacacg attttattta caccattgag cctagacaac gtgaaaggaa
    27541 tcgttgacaa aatggtggcg caattggcac aacgtctgga acatcaagaa atcctgctga
    27601 caatcagtga tgaagccaaa acgtggattg ctgaaaatgc ttatgaacca gcatatggtg
    27661 caagaccatt gaaacgattt atcactaagg aagtagaaac accactagct aaagaaatcg
    27721 ttgcaggaca tgttatgcct aaatcaaaag taacgattac tttacttgat ggacaacttc
    27781 atttcaaaac agaagagcta gaagaaatcg tctaatcttc cattgagact ataaaagaaa
    27841 aaaggggtct gacacaacta gtgtcagacc tccttttttc aaataaacag tgttccagaa
    27901 gtagtttctt cgaaaaataa gccgaaattt cttgaagctg cccgcttctg tcacaacctc
    27961 ttttattagg tatttttcac tttttctaat ttataacttt caaagtaacg ctcatctttt
    28021 ttactttgag accatgcaaa caatcccatt aaggcagcac ctatcgtatt tgtcagcaag
    28081 tcgcccatcg tatccatcaa tgctgctcgt ccgacaaaca gtgtgccatc agaagcagca
    28141 aaacgttgaa gattcatgcc taagaactgg tcgcataaga attcccagaa ctcccaaaac
    28201 acgccgcaaa gtccagcaaa cgcaaagccg aataagagga acaaccaagg actggttttt
    28261 gatatctcag catctttcat caagtaacca atcaatccat agccaagagc tgtcaggacc
    28321 attggactca ccgcatgtaa gattttgtcc caaaaagaga tgatactgat caaatgcatc
    28381 cctgtgccta aaaagacaga gatgaacaag aaaaaccagt aaaacaaaac gattgctgct
    28441 ggtattttaa gtttgaaaac tttcaaaatg acttgtggca tgaaaatcaa tgcaatccct
    28501 aataaagatt ccacgataaa tgtgattcct tgagaagtcg aatatttttg tgtgataaat
    28561 tcataaaaat tatagcagac aacgaataca ccaaaagcaa gtaaagctat ttgcaccttt
    28621 ttgatttctt taatcactga aagtccctcc atctaaaaat ctttttactt ctgaaagcaa
    28681 ctcaatttct gaagaaacca cttttccgtt caatttgatc aaacctattg tatataagtt
    28741 caggtagtga aattgatttt cagctgtttc ttgtagtgca gcgatttttt tcgggtgggt
    28801 tccgccttgc tgtcgagtat cagtatataa gccgatcact gggatgcctt tcgcataagc
    28861 cacaccgatt tccgatgcta ccccggcatc gatcgtcagc ccatctagta aagcaatcat
    28921 taaatcacta gccaacactt tttctgtgtc agcaagagca atcattcgac tgtctgcata
    28981 agcagttttg tcattgattg cttcattttc ttgaggaaga tagatcgaaa gctcagaaga
    29041 gagctctctg atttctttaa ctaactgttg gttatataac aaatcacttt tagcaaacat
    29101 tggtgcggca aaataaattt tcatctaaac atcatccttt ttatcaaaaa taatcgctaa
    29161 agtaagcttc catgtttgga atgatacgct ctagtgactt cagtgtttct tcatctgttt
    29221 ctaaccgttc gattcttgcc aaataagaag cacggcgata attcatcatc aagcaagtca
    29281 atgtttggat atccagtcga acggattttc ctataggttc atttaagaca cggacttgtc
    29341 cctgttcatc ccatgttaaa gcgaagacac cattattcca aggagcgaca ggatcttcaa
    29401 tgatgaaatg aaaagcatca gctgttccaa caaacggaaa ccgttgaagg aattctttta
    29461 catcaacgat ccgagccata aaatatggtt cgatctgctc tttgatctga ctatcttcaa
    29521 tcaaaaatgc taaaggctca tttttataaa tatctccatg tacccagtag atcatggaaa
    29581 aatgtgcgga aataaagttc cataagccat ttcgagcttc ttgatcaaga tagaacattt
    29641 cttttacgtg aaatatttct tcagctaccc agtagaacag aacacctctt ggttcatggt
    29701 tgctatcgta gtagacagcg gctgtgcgct catcttcatt ttcgaaacgc caatactctt
    29761 cccaatgaaa atcactgcgg aataaagcac catggttttg ttgggcaaat ttgtcataaa
    29821 cggaaaatac gtctggatga tcaactggta atcgctcaac cataccactt actgggattt
    29881 gtttaggtag ctgtgtatct ctgattttaa aggaaagttt atcagacatg atttcccatc
    29941 ctttacgccg atagtagggg atactgtaag gatacaaata agagatccat tgcttattct
    30001 tgcgcatatt atccaatgca acgataatta gatcttgcat caatccatgg ttagcatatt
    30061 ctggataagt tccaactcca gtcacaccac ccattttata acgtgcaccg tgaatattga
    30121 cttcacaagg atagatagcg atttgagaga tcagtttatt ttcgtggaac catccgaaca
    30181 ctttcgatac ttccaaaatc ggttttttgg aacggataaa agctcgttta ttctcaaacc
    30241 cgctttcctc gatgtctgct tcagtcactt ggaacacata ggaaagcaat tcattgaatt
    30301 gatcgatatg ttcttgagtg accggtttta attcaagatt ttctctaaaa tctttttgat
    30361 ccattttttc atctccattt attagcttga taacagtata gcaaaattct acaaaaaaaa
    30421 cttctatagc tgactaatta aaaactcttt aaattactgc ttttcgttgg tataatggaa
    30481 agtgctgatt atatgaaaag aggagacgct atggatataa agaaaatgaa agaacgccaa
    30541 gagcagattc gtaatttttc gatcatcgct catatcgacc atgggaaatc tacattggcc
    30601 gatcgtattt tagaaatgac gcacactgtg acgtctcgcg aaatgcaaga tcagctgtta
    30661 gactcgatgg atttggaacg tgaacgtgga atcacgatta aattaaatgc tgtagaactc
    30721 aattacacag cgaaaaatgg agaaacatat atctttcact tgattgacac cccagggcac
    30781 gtcgacttca cctatgaagt ctctcgaagc cttgctgcct gtgagggtgc cgttttagtg
    30841 gtcgatgcag cacaaggaat cgaagcacaa acattggcca atgtttattt agcgttagac
    30901 aatgatttgg agatcctgcc agtcatcaat aaaatcgacc tacccgcagc agatcccgaa
    30961 cgtgtccgtc aagaaatcga agacgtaatc ggaatcgatg ctagtgaagc tgtattagcc
    31021 agtgccaaat cagggattgg gattgaagat attctcgagc agatcgtgga atatgttcct
    31081 gcaccaagtg gagacttaga tgcaccgcta aaagctttga tctttgactc tatttatgat
    31141 agttaccgcg gtgttgttct aaatgtccgg atcacggacg gtatggtcaa acccggagac
    31201 aaaatcaaac tgatgagtaa tggcaaaaca tttgatgtta cagaggtcgg ggtcttctcg
    31261 ccaaaagcag ttgctagaga ctttttgatg gtcggtgatg tcggttatat cacagcaagt
    31321 atcaaaactg tccaagatac acgagtaggg gatacagtaa cgttagcaga caatcctgcc
    31381 gctgaagctt taccaggtta ccgaaaaatg aatccgatgg tttattgtgg tctttatcca
    31441 attgatactt ctcgttacaa cgatttgcgg gaagctttag aaaaactgca attgaatgat
    31501 gctgctttac aattcgaacc agaaacctct caagcattag gttttgggtt ccgctgtgga
    31561 ttcctcggac ttcttcatat ggatgtcgtc caagaacgct tggaacggga attcaatcta
    31621 gaattgatca cgactgcacc atccgttatt taccatgtta ataaaacgga tggtacgact
    31681 gctacagtag ataacccggc agattttcct gaacctgtaa cgattcagga tgtagaagaa
    31741 ccattcgtca aagcacagat catggtccca aatgattttg tcggtgcagt aatggaactt
    31801 tctcaacgaa aacggggaga attcattacg atggattatt tagatgatta ccgagtaaat
    31861 gtcgtctata atattccgct ttctgaaatt gtctttgact ttttcgacaa attgaaatca
    31921 agcacgaaag gctatgcctc attggattat gaaatgtctg gttatcaaaa aagcaagtta
    31981 gtgaagatgg acatcttgct gaatggagaa aaagtcgatg cattaagctt tatcgttcac
    32041 cgagactttg cctatgaacg tggaaaagca atcgttgaaa aactgaaaaa actaatccca
    32101 cgccaacaat ttgaggtgcc aatccaagca gctattggac aaaaaatcgt tgcccgttca
    32161 gacatcaaag ccttgcgtaa aaacgtcttg gctaaatgtt atggtggaga tgtttctcgt
    32221 aaacgtaaac tcttagagaa acaaaaagaa gggaagaagc ggatgaaaca aatcggctcc
    32281 gtcgaagttc cgcaagaagc cttcatggcc gttctgaaaa tggacgagga cgaaccgaag
    32341 aaatagcaag ggtttgtttg aaaacagtgt aaaaaataaa ccatgtttac atggatttgc
    32401 caccggtttg ccaccggtg
//
LOCUS       DAFCNJ010000028        32178 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000028 DAFCNJ010000000
VERSION     DAFCNJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.029"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(407..766)
                     /locus_tag="LNE35_001567"
     CDS             complement(407..766)
                     /locus_tag="LNE35_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBK6910730.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="LNE35_001568"
     CDS             complement(796..1137)
                     /locus_tag="LNE35_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910731.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="LNE35_001569"
     CDS             complement(1134..1808)
                     /locus_tag="LNE35_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK6910732.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="LNE35_001570"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="LNE35_001570"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBK6910733.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="LNE35_001571"
     CDS             complement(4322..5512)
                     /locus_tag="LNE35_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6910734.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="LNE35_001572"
     CDS             complement(5533..6060)
                     /locus_tag="LNE35_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBK6910735.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="LNE35_001573"
     CDS             complement(6107..8896)
                     /locus_tag="LNE35_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK6910736.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="LNE35_001574"
     CDS             complement(9043..9243)
                     /locus_tag="LNE35_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6910737.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="LNE35_001575"
     CDS             complement(9696..10016)
                     /locus_tag="LNE35_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910738.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="LNE35_001576"
     CDS             complement(10519..11637)
                     /locus_tag="LNE35_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910739.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="LNE35_001577"
     CDS             12108..13415
                     /locus_tag="LNE35_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBK6910740.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="LNE35_001578"
     CDS             13536..14735
                     /locus_tag="LNE35_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK6910741.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="LNE35_001579"
     CDS             complement(14762..16030)
                     /locus_tag="LNE35_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBK6910742.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="LNE35_001580"
     CDS             complement(16200..16841)
                     /locus_tag="LNE35_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBK6910743.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="LNE35_001581"
     CDS             complement(17250..17753)
                     /locus_tag="LNE35_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6910744.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="LNE35_001582"
     CDS             complement(17876..18640)
                     /locus_tag="LNE35_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBK6910745.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="LNE35_001583"
     CDS             18748..19023
                     /locus_tag="LNE35_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBK6910746.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="LNE35_001584"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="LNE35_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK6910747.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="LNE35_001585"
     CDS             complement(20579..20980)
                     /locus_tag="LNE35_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6910748.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="LNE35_001586"
     CDS             complement(20994..21803)
                     /locus_tag="LNE35_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6910749.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="LNE35_001587"
     CDS             complement(21790..22680)
                     /locus_tag="LNE35_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6910750.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="LNE35_001588"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="LNE35_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="LNE35_001589"
     CDS             complement(23348..24289)
                     /locus_tag="LNE35_001589"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HBK6910751.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="LNE35_001590"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="LNE35_001590"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK6910752.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="LNE35_001591"
     CDS             complement(25274..26470)
                     /locus_tag="LNE35_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6910753.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="LNE35_001592"
     CDS             complement(26488..27216)
                     /locus_tag="LNE35_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6910754.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="LNE35_001593"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="LNE35_001593"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK6910755.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="LNE35_001594"
     CDS             complement(28628..29086)
                     /locus_tag="LNE35_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6910756.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="LNE35_001595"
     CDS             complement(29083..30546)
                     /locus_tag="LNE35_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6910757.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="LNE35_001596"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="LNE35_001596"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK6910758.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="LNE35_001597"
     CDS             31602..>32178
                     /locus_tag="LNE35_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6910759.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6921 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DAFCNJ010000029        31842 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000029 DAFCNJ010000000
VERSION     DAFCNJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.030"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(151..2304)
                     /locus_tag="LNE35_001598"
     CDS             complement(151..2304)
                     /locus_tag="LNE35_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBK6910760.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="LNE35_001599"
     CDS             complement(2598..3446)
                     /locus_tag="LNE35_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6910761.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="LNE35_001600"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="LNE35_001600"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBK6910762.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="LNE35_001601"
     CDS             complement(5947..6126)
                     /locus_tag="LNE35_001601"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBK6910763.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="LNE35_001602"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="LNE35_001602"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK6910764.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="LNE35_001603"
     CDS             8274..10553
                     /locus_tag="LNE35_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6910765.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="LNE35_001604"
     CDS             complement(10646..11293)
                     /locus_tag="LNE35_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910766.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="LNE35_001605"
     CDS             complement(11472..12191)
                     /locus_tag="LNE35_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6910767.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="LNE35_001606"
     CDS             complement(12322..13473)
                     /locus_tag="LNE35_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBK6910768.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="LNE35_001607"
     CDS             complement(13690..14430)
                     /locus_tag="LNE35_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6910769.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="LNE35_001608"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="LNE35_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBK6910770.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="LNE35_001609"
     CDS             complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="LNE35_001609"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBK6910771.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16041)
                     /locus_tag="LNE35_001610"
     CDS             complement(15391..16041)
                     /locus_tag="LNE35_001610"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBK6910772.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="LNE35_001611"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="LNE35_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBK6910773.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="LNE35_001612"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="LNE35_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBK6910774.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="LNE35_001613"
     CDS             complement(17484..18011)
                     /locus_tag="LNE35_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBK6910775.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="LNE35_001614"
     CDS             complement(18191..18976)
                     /locus_tag="LNE35_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBK6910776.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="LNE35_001615"
     CDS             complement(18969..19838)
                     /locus_tag="LNE35_001615"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBK6910777.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="LNE35_001616"
     CDS             complement(19839..21218)
                     /locus_tag="LNE35_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBK6910778.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="LNE35_001617"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="LNE35_001617"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK6910779.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="LNE35_001618"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="LNE35_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBK6910780.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="LNE35_001619"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="LNE35_001619"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBK6910781.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="LNE35_001620"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="LNE35_001620"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBK6910782.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="LNE35_001621"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="LNE35_001621"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK6910783.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="LNE35_001622"
     CDS             complement(25176..25400)
                     /locus_tag="LNE35_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK6910784.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="LNE35_001623"
     CDS             complement(25453..26415)
                     /locus_tag="LNE35_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBK6910785.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="LNE35_001624"
     CDS             complement(26425..26865)
                     /locus_tag="LNE35_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6910786.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="LNE35_001625"
     CDS             27171..28136
                     /locus_tag="LNE35_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK6910787.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="LNE35_001626"
     CDS             complement(28193..28423)
                     /locus_tag="LNE35_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBK6910788.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="LNE35_001627"
     CDS             complement(28509..29396)
                     /locus_tag="LNE35_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBK6910789.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="LNE35_001628"
     CDS             complement(29485..30708)
                     /locus_tag="LNE35_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6910790.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31771)
                     /locus_tag="LNE35_001629"
     CDS             complement(30725..31771)
                     /locus_tag="LNE35_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6910791.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8794 a         6970 c         5488 g        10590 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agccctattt tt
//
LOCUS       DAFCNJ010000030        31647 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000030 DAFCNJ010000000
VERSION     DAFCNJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.031"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(233..2125)
                     /locus_tag="LNE35_001630"
     CDS             complement(233..2125)
                     /locus_tag="LNE35_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910792.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISGQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2122..3849)
                     /locus_tag="LNE35_001631"
     CDS             complement(2122..3849)
                     /locus_tag="LNE35_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910793.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3865..4515)
                     /locus_tag="LNE35_001632"
     CDS             complement(3865..4515)
                     /locus_tag="LNE35_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK6910794.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4719..5015
                     /locus_tag="LNE35_001633"
     CDS             4719..5015
                     /locus_tag="LNE35_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK6910795.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5187..5807
                     /locus_tag="LNE35_001634"
     CDS             5187..5807
                     /locus_tag="LNE35_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBK6910796.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5899..7065)
                     /locus_tag="LNE35_001635"
     CDS             complement(5899..7065)
                     /locus_tag="LNE35_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6910797.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7077..7430)
                     /locus_tag="LNE35_001636"
     CDS             complement(7077..7430)
                     /locus_tag="LNE35_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK6910798.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7558..8580
                     /locus_tag="LNE35_001637"
     CDS             7558..8580
                     /locus_tag="LNE35_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBK6910799.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8599..8760
                     /locus_tag="LNE35_001638"
     CDS             8599..8760
                     /locus_tag="LNE35_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910800.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9019..9453)
                     /locus_tag="LNE35_001639"
     CDS             complement(9019..9453)
                     /locus_tag="LNE35_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910801.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9563..11422)
                     /locus_tag="LNE35_001640"
     CDS             complement(9563..11422)
                     /locus_tag="LNE35_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK6910802.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11540..12700)
                     /locus_tag="LNE35_001641"
     CDS             complement(11540..12700)
                     /locus_tag="LNE35_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBK6910803.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13007..14431)
                     /gene="araA"
                     /locus_tag="LNE35_001642"
     CDS             complement(13007..14431)
                     /gene="araA"
                     /locus_tag="LNE35_001642"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBK6910804.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14447..15145)
                     /locus_tag="LNE35_001643"
     CDS             complement(14447..15145)
                     /locus_tag="LNE35_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK6910805.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15102..16763)
                     /locus_tag="LNE35_001644"
     CDS             complement(15102..16763)
                     /locus_tag="LNE35_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBK6910806.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16981..18381
                     /locus_tag="LNE35_001645"
     CDS             16981..18381
                     /locus_tag="LNE35_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBK6910807.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18633..19712)
                     /locus_tag="LNE35_001646"
     CDS             complement(18633..19712)
                     /locus_tag="LNE35_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6910808.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19798..20757)
                     /locus_tag="LNE35_001647"
     CDS             complement(19798..20757)
                     /locus_tag="LNE35_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK6910809.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20769..22280)
                     /locus_tag="LNE35_001648"
     CDS             complement(20769..22280)
                     /locus_tag="LNE35_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK6910810.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22302..22865)
                     /locus_tag="LNE35_001649"
     CDS             complement(22302..22865)
                     /locus_tag="LNE35_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBK6910811.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22871..23734)
                     /locus_tag="LNE35_001650"
     CDS             complement(22871..23734)
                     /locus_tag="LNE35_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6910812.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23748..24644)
                     /locus_tag="LNE35_001651"
     CDS             complement(23748..24644)
                     /locus_tag="LNE35_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6910813.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24819..26108)
                     /locus_tag="LNE35_001652"
     CDS             complement(24819..26108)
                     /locus_tag="LNE35_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6910814.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26080..27597)
                     /locus_tag="LNE35_001653"
     CDS             complement(26080..27597)
                     /locus_tag="LNE35_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK6910815.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27795..28685
                     /locus_tag="LNE35_001654"
     CDS             27795..28685
                     /locus_tag="LNE35_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBK6910816.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28758..29582)
                     /gene="nadE"
                     /locus_tag="LNE35_001655"
     CDS             complement(28758..29582)
                     /gene="nadE"
                     /locus_tag="LNE35_001655"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBK6910817.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29594..31066)
                     /locus_tag="LNE35_001656"
     CDS             complement(29594..31066)
                     /locus_tag="LNE35_001656"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBK6910818.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         9260 a         6643 c         5475 g        10269 t
ORIGIN      
        1 tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg ttgtaaaatc
       61 agcggtttga cctgagtatt agagatacaa aacggaaaaa tagcttctca tattttgccg
      121 ttttttgagc taataccgaa ggacagctga ttgaacaccg tttattcgga aaaagggact
      181 gtgacacgac ttttgtcaca gcccctaaaa aaaataataa atttgtacta ttctatgaag
      241 cttcttcttc agaaaactga ctgttataaa gatctgcata gaatccgtct ttttccatca
      301 atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt catcacaata atgttgtctg
      361 catcgcgtat tgtagacaag cggtgagcaa caacgaaact tgtccggttt tccaataggc
      421 ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc tacactagat gttgcttcat
      481 caaggatcag gacatctgga tttgctaaga atgcccgcgc aattgtgatt agctgacgct
      541 gcccttgcga aatattgctt gcatcctcgt ttaatacagt gttatatcct tctggcaatt
      601 tacgaacaaa atcgtctacg tgagcagctt tagcagcctc aatgatttgt tcgtctgttg
      661 catcgtcgtt cccatatttg atattgtcat agatacttcc tgtaaacagc caagtatctt
      721 gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga aagatcacgg gtatctacac
      781 catcgtaacg aatactacct ccagaaacat cgtagaaccg ttcaagaagg ttgatcagtg
      841 tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat ctcaccaggt ttgacatcca
      901 agttaaaatc agtcattagc aagttatctg gtgtgtagcc gaattgaaca ttttcaaaac
      961 gaactttgta tggtgtatct ttttcttctg gaaggccaga tttttcattg gacatctctt
     1021 cttcatccaa cacttcaaat acccgttcgg ctgaagcaac tgttgattgg atagtgttca
     1081 tcaaacttgc aagttgagtg attggttgag agaactgtgt tgtatattgc aagaacgctt
     1141 gaacatcccc taatgtcatg ttcccatttg caacttttac tccgcctagt acagcgacga
     1201 acacatagcc tagattttta acgaaattca tcaacggcat gatgattgcc gaaataaatt
     1261 gagctttcca accagcggca tacagattct gattttcttt ttcaaatact tcctggtctt
     1321 tttcagtatg attgaatgtt ttaacaatgg tatgaccacc atatgtttct tctacttggt
     1381 tattcaataa acctaaactt ttttgctgat ctgcgaaaaa cacttgtgat ttcggcgcta
     1441 cgatcatcac gatgatcaag cttaaaggga tcatcaataa cgcgactaac gtcaattgcc
     1501 cgctgattga aagcatcatc acgatgaccc caatcaatgt gactgcacta gttacaaatt
     1561 gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat atcattgatt gctcgagaca
     1621 tgatatcccc atttgtatgt gtatcataat aggaaatcgg tacacgattc attttggctt
     1681 ccaaatcatg acgtaattca taaactgttc gttgggaaac acgtgtcata ataaattgct
     1741 gtaaaaagtt gaatacagca gaaatcaaat acatcacgat tactgtcgca atgatctgcg
     1801 cgattttatc aaagtctatc ggaaattttg ttacttgttg gccttgttgc atctgttgag
     1861 cgccagccat tacacctttg aatatttctg tggttgcttt ccccaagacc tttggtgttt
     1921 ggatttggaa gatcacggct gctattgcta gtacaaatac agcgatgatc gcaaccatac
     1981 gtttagacat atagccaaac aatcggcgta ccgttcccca gaaattttta ggcttttcac
     2041 cttttacacc gagatttctg ccaggtccac catgtcctgg accgcctgct ggtccatgag
     2101 agggacgtgt gtttgtttta ctcatgctaa gtcctcctct cttagttgtg aatgaacgat
     2161 ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt gttcctttgc caaccagttt
     2221 tccttcatct agcacaagga tcatatctgc atccattact gtacttactc gttgagcaac
     2281 taaaacggtg atactttctt tcatattcgt tttcaatgct tggcgaagat tggcatccgt
     2341 tttgaaatcc aacgctgaga aggaatcatc aaacacatag atatctgctg ttttgactaa
     2401 tgcccgtgca attgccagac gctgtctttg tccaccagag aagtttcctc ctccttgttc
     2461 cacatggcta tctaagccat cttccatctc tgaaacaaaa tctttagctt gtgcgatttc
     2521 taatgctttc caaatcattt catctgttgc atccggctta ccatactgca tattgtctcg
     2581 gatcgttcca gagaacagaa ctgctttttg aggaacaaag cccattaatt ctcgcaaatt
     2641 atattgagtc atgtcactaa tgtccgttcc atttatttgg atcgaaccag attcgatgtc
     2701 atataatcga gggatcaaat tgactaaggt cgttttacct gaaccagtcc cccctatgat
     2761 tgcgacgatc tcgcccgact tggcttggaa atcaatatct tccaatgcaa gtttttctgc
     2821 tccatgatat cgataattga catgattgaa ggccaatgtt gcgttttttc cagcaaaaga
     2881 aactgtttta ggattttcag ggtcatggat accgattttt tcatccaaca cttcattaat
     2941 acggtcagct gaagcttgtg cacgaggcac catgataaaa atagcggaaa gcatcataaa
     3001 gctgatcaag atctgcatcg cataagtcat aaatgcaatc agattaccga cttccaaagt
     3061 catatctgct atgtagtgtc caccaaacca agtgattgct acgtttgtac cgctcatgat
     3121 caatgtcatc attggcatca tcaatgcgac gatcgtatta actttgattg ccgtatttgt
     3181 ataatcttta ttcgctaaat caaaacgatt ttcttcaaag cgtgtcttat cgaatgctcg
     3241 aatcactctg acccctgtca atccttctcg aaataccagg tttaaacggt cagttttctt
     3301 ttgcatactt ttgaataatg gaacagcaaa gtacatgatt ccgccgatca aaataatcat
     3361 tactgggatg acaactaaga agatttttgt caactgatga tctttttgat aagcaagtat
     3421 gctcgcgcca atcaacgtga ttggtgcatt gatcatcata cgtaaaaaca tttgtgtaac
     3481 catttggatt tgagtcacat cattggtcgt tcttgtgatc aaagaggccg ttccatattt
     3541 atcaaacgca tcttttgtca gactctctga ttttttatag acatccgtac gcaattgctt
     3601 acccagtttc tgtgattcac gagtagcaaa aaatgtattt gcgatagcag ctaagatact
     3661 gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa atataatcga catctccttg
     3721 cgctacccct ttatcaataa tattggaggt aagtgtcggc aaatataaat ctgccagtac
     3781 ttggatcacc atgaaaactg cagctgcaat tgctgaagtc aaggagatcc gttttacgat
     3841 ttttagcatc tagttccctt ctttctattt atatactccg tttttcagcc aagaaagttt
     3901 caaattgaat tcactgattg cttgctctac tgagtaatct tcagatgttg aaaataagcg
     3961 atacgaatgc gcaattgttg taaatacagt attcatcact aattggatca gtaattccaa
     4021 ttcatgatca ttttttactt tcagcttcgt ccgatcgatc aattcaagca actcttttgc
     4081 atcttgtttg tgttctttat gtggatcgtg aggcgttttt tggcgactcg tatgaggagc
     4141 gacttttctt gaggcacggt aatccatgtt cataaacaag ttacgataaa aagaagcatt
     4201 ctcgccttcc aaaatctcat aaatcatttt agaaaaatag gattcgaatc ctttgaatag
     4261 atcaccatcg gattctttca ttagttgtaa catatcttta gagctgtcac gtctcattgt
     4321 ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa tactgataga aactgcctct
     4381 agggatatcc gctaatttga cgatattagc aatcgaagct tcttttaagg gtactctaga
     4441 aaactcgatt ctcgcagcct cgattagtct tttttgtttt tctttaggca aatggaaaaa
     4501 tgtctgtgtc ggcatatgga tcctcctttc tttcaagatg acacattgtc atatgacgct
     4561 gtgtcattat aacacctcct acctgttttg caagacattt gcatagtttt tttcattatc
     4621 atccgataca ttaaaaaagt gataagagga atccttccat ttgaattaat gagagttgcc
     4681 cattaaaata gccacatact aaacaaagga tgagataaat ggcccaatca attagtgtcc
     4741 aagacttcaa taaattgcct ctcgatgctg acagcatcgt tttagataca agaaatacct
     4801 tagattacac aaaaaaccac ttaagccact cgctttctat ccccgctgct atcttgccaa
     4861 gacaattaaa aaatctagat ccaaagaaac tttattatat tctaagctat acaggcaggc
     4921 gatcagaagc attagccgat caacttactg ctaatggttt tcgtgctgtt tatatcattg
     4981 gtgggatgga agctcttcag tcgagtgttg cttgaatatt gcttgttaga ttcaaaagag
     5041 gctgtgacaa aaaatcgtgt cacagcctct tttataccct gtcttttttt agttatttat
     5101 tcttcaacct tatcaatatg acttgatagc cttttctttt tctcatagtt ataacaatca
     5161 tagcaataaa aggagaaggt aagcatatgg catctgtttt agtagtcaaa ggtcatcccc
     5221 taactgcaga agaatcgcgc acagttaagg cattgacgtc ttttttaaca agttataaag
     5281 aaaatcatcc agatgatgaa gtgactgttt tggaacttta tcgcgatgat attccggaaa
     5341 tcgatgaaga attattatca ggttgggaag cattacgtgc tggtgcagaa ttcacttcct
     5401 tatcagaaag tcaacaacaa aaaattgctc gtttcaatga attgactgat cagttccttg
     5461 ctgctgataa aatcgttatc gcaaacgcct tatggaatct aaatatccca acaaaattaa
     5521 aagcttggtt cgataccgtc aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc
     5581 cagaaggatt agtgacaggt aaaaaagcac tgcacatcca atctaatggc ggtgtataca
     5641 acggacaaga ctttgcctct caatatgtaa aaggtatttt aaactttgta ggcatcgatc
     5701 aagtagatca gctattcatc gaaggaatcg attatgaccc tgaccgtgca gatgagttga
     5761 tgcaaaatgc attagataaa gctgctgctt taggtaaatc attttaaaaa ttgagataaa
     5821 tgcatacaaa agagacagag gaaaaaatct tcaaagcagc atttgtacct tggatatttt
     5881 cccatctgtc tctttttgtt attctttctg ctttttcaac ggattttctt cataaagaga
     5941 agagattgtt gtgtaccatt caaaaacatg agtaatcact actttagctg ctgcataaac
     6001 aggaatcccc aagatcacac cgatgattcc gaacaatttc ccagaagtca gcagaactaa
     6061 taaaatcgtt actggatgga ttgctaactg attccctagt actaatggag agatcaagcg
     6121 tccttctatt gtctgctcta ctgcaaaaac gatcagtact ttgatgatca tgaatggacc
     6181 tgctacaatg gctaaaaaga tagccggaat catcgctaaa aatgatccta agtaagggat
     6241 caggttcaaa aaacccgcaa taatccccaa cgtgacagca tactctaaac caatgaccgc
     6301 aaaaccaatc ataaacatga ttgccacagc aaaagccaca gttagttgtc ctcttatata
     6361 agaagaaact tgactattca tttcactcag cacattcaac gtcggttttt gcatgcgtgt
     6421 cggtaaaaat ttgacaaaat aaggtgctaa ttgtttaccg tctttcaata gataaaataa
     6481 gataaacggc atcgtcagca gagccactaa aatagtcgct accgcgccga caaaacttcc
     6541 aagactctga actgtactct tagatatatc ttggatcata tcgcctgccg aattcatgaa
     6601 cttatcacct gctgtttcca actgttcgcg aaattggttg aacagcgggt cccgcaaaat
     6661 ctcggttaat ttattatcaa tcgtatctac atattgaggg aagttactga tgaagctcac
     6721 tgtttgttcc tgaatcttag ggatgatcac tacgctcccc catgcgatta gtgcaacaac
     6781 caaaacaaac agcccaatga tactgtaaag ccttggtact tttcgctttt caaagtaatc
     6841 aactaccgga ttcatcaagt aatacaagat tcctgctaaa atgataggca gacctacgat
     6901 tgctaaaaat tgccagattg gtgcaaacaa atatgaaact tttgtaaata aaaagacgat
     6961 cagcaatatt aacaatacaa caagtaaggc tgtgactact tgattattta aaaaccagcg
     7021 ccaaaaccac gataccggtc tgttcttctt attttttcct tccataattc ctcctgttag
     7081 ttataaaaaa tttggcttcc caaaccaata gcaggcagtc tatgcggatg aagatagatt
     7141 ccatttgcaa tttgtccttc ttctggacaa gcatcaaaaa tcaatgatac gtgagctact
     7201 gtctctaaat tcccattggc catcggaccg acataatcga tcgtatatac ctgatcatca
     7261 aaagcaattg ttccgcctgc tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc
     7321 accgaatatt ttcgtaaggc agctgttgct gttttatcga acaaaatcag catgggttct
     7381 tccttgttta ttgcttctgt cccaatttct gtaatctttg cctgaatcat tgcactctct
     7441 cctttaattg ctttcaaaaa cagtataaca cattctcatt ctttagccgt gtggaataat
     7501 tatgtgatag aataaaaacg aaaactattt cattacaatt aggaggaatc agctcatatg
     7561 atcgaaaaga taattttagg aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc
     7621 gaccttgata cagaaaaaga agaattagcc aacctttcat tagctgttga agaaaatagc
     7681 cctacctatg tagcaaaaag taaagctggc aatctttaca cagttactag tgtggatgga
     7741 ttaggtggtg ccggtgcgta cgataaggat tttcattttt tgaatgcggt aacagagtct
     7801 ggcgctcctt tgtgttatgt agcggttgat gaagcacgtc aattactata cggggcgaac
     7861 tatcataaag gtgaagtaaa tgtctaccat atattaggag atggcgaact gaaagcagca
     7921 gattcaatat tccatcaaga agcgacaggt cctcataaaa accaagatca tgcgcatgtc
     7981 cactatacgg atctgactcc tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt
     8041 gtgtatactt atgatgtgtc cgaaaacggc aaattgaatc ttgtaactac cttcactgca
     8101 gagccaggaa caggccctcg ccatcttgtc tttcatccaa atggacaatt cgcttattta
     8161 tttggtgaat tagacagcac agtccgcgtg ctttcttata ataaagaaac aggtgctttt
     8221 gaagaattac agaaaatatc gaccttgcca gaagaattcg acggtgaaaa tggaggggca
     8281 gctatccgca tttcaaatga tggcaagttc ctttatgctt ctaaccgagg acataactcg
     8341 attgctgtat tcgcagtttt tgaagatggt tcccacattc aaaacaagca aatcatctca
     8401 acagaagggg attttccccg cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt
     8461 gccaatcaga acaccgataa tctgacatta tatcgccgtc atcctgaaac aggattactg
     8521 gaattactga aaaaagatgt ctatgtacca gaatgtgtct gtgtttattt tgatcgttag
     8581 taagaaaagg agaatgaaat gaatcagcaa gacgtattag acagactacg ggaagaacta
     8641 aatattcctt tctttgatgg gaaaatcgaa gataaagagt atagcgaaga ggaatatcag
     8701 aaactcaaag ccgatttaca gaactacttt gaacaatatg tacagaatgt agaaaactaa
     8761 tcagctttcc atactatctg aaaagaacat caggacaaga cttttccgtc tatcctgatg
     8821 ttcttttcta caaataaaaa accgccgaga tggttagact cgacggaaaa ggagtattta
     8881 ctcgattttg agtaagtaca aggttgttgt tggttatagg tatgtcatta tcataaagga
     8941 ttttgatcac tctatcataa aggtaatatt acatttatat tagataactc tagtttttat
     9001 tgttatcaaa gatttctttt attttttcaa caacaaatta gtcatttttt ggatggcagc
     9061 tgtcaattgg tctgtcgctt ggtcttgttt tgcttcagat gtattgaata gatcttcatc
     9121 tacccctgat tcaagatcga tactttggat catttcttca cagcctgcta catatgaaag
     9181 ataagctttt tcaaaatttt tatgaatgcc cattgctttt gctggtggac gcaaagtact
     9241 gactttctta agcattgccc gatatttttt tgttccttct tcaaataaca ggatgatctc
     9301 ttcaagagtc gatttgtcca gttcacttgt ttttccttca tcaattgcgt ttctgatcac
     9361 ttcatatttc ggatgcattc cttctccgat tgcttctgtt tccttgacga tttgatcgat
     9421 ggactgcaca taatagccaa tattcggtaa catgttcgtt ttcctacttt ctccattgtt
     9481 gttattattt attctacttg tttttatcct caaatgacaa gtacttgaaa aaatttaatg
     9541 gtttctatac agctcgccat tcttattcgt gctcaaagaa accatctgct aaactatatt
     9601 gtttccctgc gacaatgtca tattggatca attgatattc gtcgtaaatc tcttgttgcg
     9661 agcgattgaa ttgcgcttct ttataccact gtccgttttg atagtacagt tctcgttcaa
     9721 acgccggaag ttcctgttct aatgataaaa gcagctgata aaagccattt gttgtctgat
     9781 tcgtctgttc cattagattc gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg
     9841 tttgcttttc taattctcct ttactgtcga acataagaaa ttgtgtttca tgcaatgttt
     9901 gtttgtcatt ttttgcttgg atcgtatctg aatagatccc tggaagatga tccccccaaa
     9961 aaacaactaa agtccttcta gaaagattct tcaattcttc cgtgaactgc ttcagtgccg
    10021 tgctgctata ggaaatatct tgcagataat tctctaagtc aagtgtccca cttccatctg
    10081 aagtttttgc aggatagtct agattatcat actttccatc gtagggcata tgtgtctgca
    10141 tcgtgactaa atgaatgaac tgaggtgttt tttcttcctt gagaagtgtc attacttctt
    10201 tgtaagctga ctcatcagaa atatatggat tattctctat cgtatccgta taggtcatcg
    10261 tacgctcgct gatgaactgg tcaaatccta atgtctgata aacatcctct cttttataca
    10321 tcgaagtatt gtatggatgg atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa
    10381 cgatagaagg aagctgatcc atttttggta caagcatcgt gtaaggggtg gtgagttgcg
    10441 cattaaataa cgccatagaa aaaccagtca atgcttcaaa ttcgatattt gctgttccac
    10501 cgccatagtt ttgagaaagc atattgccac tatatgtttg atcagctact tcataataat
    10561 ctgccaatgg ctcaccagat acttctacgc cgtttaatcg actaggatct gagaaacttt
    10621 cactcatcac gaacacgatg ttcggcgact cttcctctac agttttattt ttttcatcgg
    10681 ctagcttctg gtatttttca gtgatttctt tgatcttttc tttcgaatat ccttcaggtt
    10741 catccattgg ttctaccttc agattgtaca aaaacccacc gataaaacca gtgttatagt
    10801 agttcatttt ctggctgtaa ggtatccata gtgccgtttt attatatgct tttcgcaaca
    10861 aattatcggg attgttgaaa ttactgatat accctagcag tccaattgcc acaagcagtg
    10921 tcgttaatcg gatcaattgg aatcgccttc cttttttcag gcttcgaaac atgtaccagc
    10981 aaaacaatcc tatcacggtt cctgcggcag ctaagatcac tgtaaacagc atagtgccgg
    11041 tcatatcttt cagcaatccg atttcagtga tcatcttcaa atcgtctgga taaatcggtt
    11101 cttgccgata actcattttc atataatttg caaaacctaa tacaccgatg gctacgacat
    11161 acgtaaggga acctaaaggg acagatcctg ctagtgcaat cagaaaaata agaataatca
    11221 gcaagacgag acaggctaag aagaattttt ccgtatgcca agaaaaagca aatttcaacg
    11281 caaggtctac ggatagatca ttttgacacc actgcaaaaa taaattacta aaaacaacaa
    11341 gaaaaaagat actgatgatt gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa
    11401 gtctgcttat atatcttttc aaatgcttgc tccttcattt ctatcaataa aagaatgttg
    11461 cttctcttat tttagttgta cgaaattcga aagtaaagaa cggaaagaag aattaatgaa
    11521 atccaaatat tctctttctt tatggccttt ctggtttctt taataagcgg acctttcgta
    11581 ctccagaaat tttttctagc tgttgtttca cttgattgag ctgttcaaaa tcagtactgt
    11641 ctatgtcaat caatatgtag acatgctcat tcttatggtt acgcgatata tctgcgatcg
    11701 ctagctcgtt ctcattcagc agagaaaata tctctgctaa tatattggtt ttcttttgat
    11761 aaaatatagt gaatcgaaac ggcgcttgga aaaacattcg tgcggatgga taattgaccg
    11821 cttctctaac cgtaccgaac aaaagataat cttttaaggc ttcaactgcc aaacgtccgc
    11881 tgtctgccaa agcttcctga gtcgatcccc ctagatgagg caacataagt atacggtcat
    11941 tttctaagaa ctcttcttct ggaaaatcag tcaagtattt agccagatgt tgtttttcca
    12001 atgcttgcat aacagcatat ttatcaacaa tttcacttct tcctacattg ataagtacag
    12061 catctttttt cattttttct atattcgcct gatcgatcaa tccttttgta tcttctgtca
    12121 acggcagcat gactacgatg taatccgagg tagaaagaac tgtttctaaa tctgcttgcc
    12181 gaacataatc caactgggca tcgcggatag aataacccaa gacatccatc cctaagctat
    12241 agcaacttag cgctactttt gtgcctatcg ctccaagacc aagaagaccg atggttttgc
    12301 cttgcaattc tcgtcccaca taagcacttc ttttgttttc tgcctgttct aaaatattag
    12361 gacctgttag tgtttgtacc atacgacttg cttcgattat cggacgggaa gataagagca
    12421 aacagcacaa aacaagctct tttactgcat tagcatttac acctggtgta ttcatgacga
    12481 tcgtcccgtt ttcagaagct ttttcaacgt tgatcgtgtt caccccaaca ccagctcttg
    12541 aaattgctag caaatctggt gtaagccatt catctttcac tttcgtacta cgaataacta
    12601 ttccttcggc tggcaaatca tctattattt tgaaatattc cccttgtaac ttttctatct
    12661 cttgttctgg aatagcatca atcaaatgaa cgtgatacat aaaaccccct ccttactaac
    12721 aagtatctcg aaatttctgc aattagacaa ctgtttacca tttgacttcc tagaagcaaa
    12781 aagagcagtt agagcagagt aggctgagtg ataaaaataa ggctatgaca agttactgcc
    12841 ataagcctta ttttcgagag caaaatattg agggtgagat atcttgataa atggtgttcc
    12901 gttagctgac attgataata agactaaatt tcaaaaaagt tgaaaagcaa atttcgtcaa
    12961 tctcttctta tttcttaaaa ctaagtggct ttttcacaac ctctttttat ctaataacca
    13021 ctgtttccaa ctcaactaat tttgcccacg tttcaatttg atctgttgtt agatttagcg
    13081 agacaacggt atgatgaccg ccacctgcct gaatccatga atggacacct tcatggaaat
    13141 tcggtttagt tttccataac acacgtgcaa caggtaaatt aggtgcttca gtcgttggtt
    13201 caaaggcttc tacttcattg atcagcagtt tataatgcgt accgaaatct gccatcgata
    13261 cgactacccc ttctcctgct ttcccatcaa atacaagacg cgccggatct tctctatctc
    13321 ccattgataa aggagaaaca acgattcttg gtttcgtcgc agcaagcgtc ggatctactt
    13381 ccatcatatg ggattccaag attgcttctt ggccactagc tagctcgtaa gtataatctt
    13441 ccatgaatcc tgtatctttc cctcgagcca tgattttcag caaccgatca atcgctgcag
    13501 ttttccagtc gccttcccca gcaaaaccgt atccttctgc catcaaacgt tgtacagcta
    13561 gtccaggtag ttgtttcatt ccatacaggt cttcaaaatt agtcgtaaaa gcgttatagc
    13621 ctccttcttc taagaaacgg cgaataccga tttcctgctg agcttgtact tttacatgtt
    13681 cctcccatgt cttttcttca taatcaccat agtcgaaggt atacaattcc ttgtactctt
    13741 caaataattg atcgacttct tcgtctgata cgcgatcgat tacttgtaca agatctccga
    13801 taccaaaata atcaacagtc caaccaaatt gaatctgtgc ttccactttg tctccttctg
    13861 tgacacccac gtttctcatg ttgtcaccaa aacgagccac ttttattcca aaactttcat
    13921 tataggctac tgcaacatcc atccaatcag caatctcttt ttggatctca ggtcgtttcc
    13981 agtagccaac gacgatttta ttttgtttat ttaatcgagc attgatgaac ccatattcgc
    14041 ggtcaccatg agcggattga tttaaattca tgaaatccat atcgatcgtt ttccaaggaa
    14101 tgctttcgtt atattgcgta gctaaatgaa gcaatggttt ttgtagaagc tttgtcccgc
    14161 ggatccacat ttttgctggt gagaaagtat gcatccaagt gatcacacct gctacttctt
    14221 ctcgataatt gacctctttc atgatttttg tgatcgtatc agcagtaact gctaattctt
    14281 gaaggcggat tggataaggc aattggccat tttcattcag ttcatcgacc atttcttgtg
    14341 catgtttctt tacttctcgc agtgcttctt ccccatataa atgctgtgat cctacaacaa
    14401 accaaaattc tttttctcca atattcaaca taattagcca ctccttttat ttgttttgtc
    14461 cgtaatacgc attttttccg tgttttcgta aataatgttt atctaaaatc cgttgaggca
    14521 gctcttctga aaatgaattg agctgtctgg taaataaatt catcttgcat acttcatcta
    14581 gtacgactgc gttcattacc gcactttgcg catctttccc ccaagtaaat ggaccgtgtc
    14641 cgtgtaaaag tacccctgga attgctaagg gatcaatttt gcgttctttg aatgtttcta
    14701 tgataacttt tcctgtttca tattcgtacc cgctgtcgat ttcctgttgt gtcaaaaatc
    14761 gagcacaagg taccgatcca taaaaagtat ctgcatgcgt cgtccccatc gcaggaacat
    14821 ccaatcctgc ttgtgcccag atcgtcgccc atgtcgagtg agtatggacg attcctccga
    14881 tttgaggaaa tgaacgatat aaaacagcat gagtaggcgt atccgaagag ggattgagtt
    14941 taccttcaat gatatgattg tccaagtcca caacgaccat gtcgtctgct gtcatctctt
    15001 cataactgac tccgctaggt ttgataacga aatatcgtgt ttcaggatca aaagcgctca
    15061 catttcccca tgtatatttg acgagtccgt gtttaggcaa gtcaagattt gcttggtata
    15121 cttcttcttt cagttgttcc agcattttct atcactcctt tttcttgcaa ttgatgaatc
    15181 gctgtttttt caatgtcgat cccctcacgg taacgtctca taaatatctg atagcctgct
    15241 atttcttctg ctgaaggtgt gatggtgatt ccttggtctt gtccaaacac gtcttctgcc
    15301 aagaagtttt ctaaagactt cccatgtcga tccatcatat aggctgctaa taacgcgatt
    15361 ccccacgctc caccttctcc ggctgtgtcc atcactgtaa ctggtgcctc catagcagat
    15421 gctagaatcc gctgtccaac ttctggtgtt ttaaaaatcc ccccatgccc cactaaacga
    15481 tcaatttgga cattctcaga tttcaatata tccattccga ttctcattgc accaaaagca
    15541 ctggctaaat ggatccgcat gaaattcgct aaatcaaaac ggctacctgg tgtacgtaca
    15601 aataatggcc ttccttcatt tactcctgta atgttttcac cggaaaaata accataagaa
    15661 agtaatcccc cgcaatccgt atcgccttct agcgctttgt tgaataatac cgtgaataat
    15721 ttatctctgc ttatctccat acccaaagaa ttggtaaatt cttcaaaaat actgacccaa
    15781 gcattgatat ccgaagagca gttattcgta tgaaccatgc ctactagttc tcctgaaggt
    15841 gtagtgacca tgtcgatttc cggatggaca ttttctagtt ctttttctaa tacgatcata
    15901 gcgaaggcgg aagtaccagc cgaaatgttc cctgtccgtt tggctacact gttcgtagcg
    15961 accataccgg ttcctgcatc cccttctggc ggacaaaccg ggattcctgg tctcaaattt
    16021 cccgtagggt cgatcagata agcccctgat tcgcttaact tgccagcttc ttctcctgct
    16081 actagaactt taggcaaaat gtgatccaag tcaatcgcta cgtgcgcttt ttcgattaac
    16141 tgaccaaaaa tatttttcat ttcttgatgg taggttttcg ttgaaggatc gataggaaac
    16201 atgcctgatg catctcctac gcctagtact ttctcaccag taagctgcca atggacataa
    16261 cctgcaagcg ttgtcaaaaa attaatttct ttcacatgtt cttctttatt caaaatagat
    16321 tgatacaaat gagcgatact ccatctctgc ggaatattgt attgaaattt ttccgttaat
    16381 attcgttctg cttcttcagt gattgtattt cgccaagtac gaaatggtac tagtaactgt
    16441 tccttgctat caaatgctag ataaccgtgc atcattgcac tgaacccaat cgacccgatt
    16501 gtcgtaagat tctcgccata gatttcgaat acttctgctg ccatttttcg gtaactgacg
    16561 cgcagtcctt tccaaatatc ttctaatgaa taggtccaga taccattttc tagtttgttt
    16621 tcccactcat aactaccagc ggcaatagga gtaaaagatg aatcaatcaa tacagctttg
    16681 atcctagttg atccaaactc aatccctaaa gatgtacgac catctatgac atctaacata
    16741 ctttgcgcat ttacttcctt cataatgatt taactccctc cttctcgtca aacctgatca
    16801 aaccgtcttt ttagtagcgc ttacaatgat tattgtatac ttttgtacgt acatgtcaag
    16861 cggttattta caagaataca ataaaaaaca cttgttttgt acgtacataa gttataaaat
    16921 gaaattgctt acaaaatctg atcaaccgcc ttctcgaaat tctagaagga gagaatactt
    16981 atgaaaaaag aaaaaaaagt ttcctcatcg tttatttatt tttttggcgc atttggcggc
    17041 attctatttg gatatgatat tggtgttatg accggcgcct tacctttttt acagcatgat
    17101 tggggtttgg caggtaaagc ttctttgata ggatggatca cgtcttcggt catgttaggt
    17161 gcgatccttg gcggctcttt atctggtctt ctttctgata agcttggacg acgaaaaatg
    17221 attcttcttt ctgcacttat ttttatggct ggttctgtcc tttctgcatc tgcccctcac
    17281 aatggttctt attttctgat tgcagcaagg attcttctag gtttagctgt tggcgctgca
    17341 tctgccctag ttcctgctta tatgtctgaa atggctcctg ctagactaag gggaagattg
    17401 tcagggatca atcaagtgat gattgcttct ggcatgctcc tttcctatgt tgcagattat
    17461 ttgctaaaag tgttgcctga aacgatggct tggcgcgtga tgttagggct cgctgctgtc
    17521 cccgcgctga ttttattttt cggtgtttta gctctaccag agtcaccgcg ttttcttatg
    17581 caatctggcc gcttggaaga ggcaaaaaga gtattgaact atattcggac accaaaagaa
    17641 gctgaacagg aattcgaaca aatccagctg aatgtcaaac aagaaaagac aaccggaact
    17701 tcttggcata ctttgttttt agaaaaatat cgttccttag tcattgccgg tattggtgta
    17761 gcagtattcc agcaatttca aggagctaat gctatttttt actatattcc gctgatcgta
    17821 gaaaaagcga ctgggcatgc agcgagcgat gcattgatgt ggccgatcat ccaaggaatc
    17881 attttagttg ctggttcttt gctctttttg gttattgcag acaagttcaa tcggcgaact
    17941 ttattgaaaa taggcggtag cgtgatggga ctttcattca tcctaccagc tgttttagga
    18001 actgtactgg atgctcatac aaattcgttg ttgattctct tgtttttatg tatttatgtc
    18061 gcattttatt cttgcacatg ggctccactg acatgggtaa tcataggcga aatattccca
    18121 ctagctgtcc gcgggcgagc atctggccta gcctcttctt ttaattggat tggttctttc
    18181 ctagttggct tgctttttcc tgtgatgact gcttctatgt ctcaagaaat cgttttcggc
    18241 atttttggga tcatctgctt tttaggggtt ttatttattc aagagatcgt accagaaact
    18301 cgtgggaaat ctttagagga aatcgaacaa agtgcatcta aaaaaactta tcctaaacgt
    18361 atttctatcc atcattctta aagaggttgt gacagaagtg gtcagcttca agaaataaga
    18421 aggaaccgct tagtttccag tcacaaggaa caatttcaaa aaacagcttc tcatgtttga
    18481 tgaatattgt gacttgtgac cagttggtag gagaacaccg tttagtggag aggtggtgaa
    18541 agaaacgttc agccttgagc gatgacaaaa gctctcacaa acattgcgac gagtaaccgt
    18601 aggagcaata agaggcggta aaaacccgtc tcttattttt cgttttcttt cactgtttcc
    18661 cgttcaacta gtacaggttc atagagaata tttggctgtg cctgtccttt ttcaatcgtt
    18721 gtgacgatcc accgtgcggc atcttgtccc aacttttctt ttggatgcgt caatgttgtc
    18781 aattttttat tccctaaatg gcttaaagaa gaatcgtctg ttcctacgat cgataggtca
    18841 tctggaatat gatagccatt ttctgtcaac ttatctagca ttaaactagc tatttgatcg
    18901 ttatagcaaa tgatgccatc taaagcttgt tcttctttca accgttgtac tgttttttca
    18961 gcgattgttt ctcgtgtttc cgtggtgaaa gtaatgatat cttccgtcaa aaaacgcaca
    19021 ttatactgtt cacacgcgct gataaaacct ttcatcctgt attttccttg aagatcgtcg
    19081 atttttgtga tgaacaacag acgctgatgt ccttttttta tcaaatattc tgctgccata
    19141 aaaccgactt tcacatcatc taaacaaata taagaggcat ttaactcttc ataagcagca
    19201 ttgatcatca ccatcggaat gccttgttct ctcaaagcaa cataaagtgc taagtttggg
    19261 ttatactgat tacttttcgt aggctcaacg atcaaaccat ctaccccata agcaatcatt
    19321 ttcttcaaac actctttctc caattcgtga tcgttattcg tactggctaa aagcaatgaa
    19381 tacccagatt ctcgtaattc attttcgatt cctcggataa tcgacggaaa aatataatca
    19441 gagagatatg tggtgatgac accgatcgtt tttgttctgt tcaatggtgt tttttctttc
    19501 ttatagctgt catctacata tgtgcccgac cctttttctt tacgcagata gccctcattt
    19561 accaacacag cgattgcttg ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc
    19621 ttgaattctg agggaatggt tgtatttact tcgtattcct cacgaaggat cttttgtttt
    19681 atctcttctg caatatcttg atattttgcc atttgccttg ccccttttta tttgtacgta
    19741 catatatcat aacgagtctg caaggtttat tcaagcaaaa atcaaggttt cttgtgctca
    19801 taaggcatac ggaacaggtt caccaaattc tggaaatcct tgctcattcc acgtaaagca
    19861 ttgcgcgttc gcgtgccgat taggattgtc taacggatcc cctttttggt tcttctctgg
    19921 tcgagcatga tagatcaaaa tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg
    19981 accaggacca aattgcttat ttttttcaga agaaacgaat actggctccg ataatttatg
    20041 ccatgattct tttaataaaa gattcttgtg ctcatcagcc cacagcaggc ccatggcata
    20101 gttttcatct gttgcactac cagaatacgt aatgaatatt ttcccattgc gagtaaggac
    20161 tgctggacct tcatttactg aaaaaccccg tttttcccat tcatactcag gtatagaaag
    20221 gaggatctgc ttccccttta acgtccaagg attatccatt tcggatagat aaagattaga
    20281 attcccagta atttcaggat cttgttgggc ccacacataa tatagctttc cttgagaaga
    20341 gaaagttgtt ccgtctaaac tgaaagtttc ccttttcgtt tttatttgtc ccttttctat
    20401 ccaatctgta ttcattggat ctatatgctg attttcaagt acgaacatgc ggtgatgatg
    20461 agattcatca cgtatagttt tatgattgct tgcagcaaag taaatatacc attttccatt
    20521 gatataatgg acttcaggtg cccagatcaa ttcactcatc ggtccttctt catgagcctg
    20581 ccatacaatc aggcttgcag catgttctaa ttcattcagt gactttgccc ttcttaattc
    20641 aatcacttgg tagcctggaa ccgatcctgt aaaataataa taaccatctg tatgtttata
    20701 tacccaagga tctgcccgtt gccgaacgat aggattatta taaagtgtct tcatcataac
    20761 aaatttcctc acattttctt tattctaatt acattccaag agtatttttc tatccctaca
    20821 tttaattcgt catgtttttc tattgaaaaa tagtttattt caggttttat tttttctggt
    20881 tcttcacgtg tatttttgtc caacagatcg ctactggtta atgtgtagtg ctctccttga
    20941 tctagcaaat tgaaatcatg ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc
    21001 aagaaaatag tcagctgatt gccgttatct acgatcacac tgtccacata aggaacattt
    21061 tttatctttt cacaatcata aacagggcaa atgatttgtt gtttcaatac ttgaccttta
    21121 gcatatttcg ataagtgttg catcacataa taagttggat tcctccatgc ttgtccttta
    21181 gcatctgtca gtattaaagg tattgtgttt actaacaatg attgacaact aatctttacg
    21241 cgatctgcgt gtcgtaaaat agccagccct aatgaaccaa ataataatgt atcttccata
    21301 gtaaagtaac accaatcgat gggactaccg acttcaaaat ccctatgtct tttttccggt
    21361 tgggcatgta cattccattc atcaaacgct agatacattg ttttctcact tctcaaatat
    21421 ccttttacat aatcacaagt agcaacgatt tcttcaattt gacgatcgaa tcgataagct
    21481 tttgcaaggt atgtcgctgt atcatcacgc tcatttttag gaggtttagt caagtcttct
    21541 tcatcatact tatcaatata gttatgcaaa gctaaataat ctacattctc ataagcttct
    21601 tctaatgcga tacgatccca ttcagggtac gatggtaaac gtggcgtaga gcttccaact
    21661 aagattgttt caattgaatc atccactaac ttcatgactt ttgaggcttc atttgctaac
    21721 ctaccatatt cataagcact cttagccccc acttgccatg gaccgtctag ctcattccct
    21781 aaacaccata atttaacgcc aaaaggcttt ttttgcccat tttttattct taaatcactc
    21841 cagaaggttc cttttggaaa attacaatat tctactaaat tttttgcttc atcaattcct
    21901 tttgttccta aattcaccgt gaatatcggt ttcgcttgta catgactgat ccatttaaaa
    21961 aattcgtgga gtccaaaaga atttggttct atacctcgcc atgctaaatc aatttttttt
    22021 ggtcgttgtt ctttcctacc tacagtatcc acccaatcat aaccagatgt aaagtttcct
    22081 ccaggatagc gaatgacacc taattctaat tcctttacat aagagaggac atcttgtcta
    22141 aacccgtcac catcagctgt ttcatggccc ggttcataaa tccctgtata gacgacactt
    22201 cccatatgct caacaaatga gccaaacagc cgagaactga tctctcctac cgtaaaatcc
    22261 tctctgattt ttactttcac ttcttcgtcc aacagctttc cctacctttc atttcctata
    22321 gtcaagataa tagatacaag tttccatacg ccaccagcaa agataaaaat aaaaggaggg
    22381 aaaagaaata tgagcagtac aaatcctaat aacaatacgc aacaaagaat atttctcaaa
    22441 aaatcaaaaa atactttcca tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg
    22501 ttttcagaaa tcacatatat ccaggataaa agaaacatgg aataaataag taaaactaca
    22561 tatccactaa ttgaaagtac gattgtctgt tcttctaata attgattaac aagaaaataa
    22621 ataatgatcg ttaacagact aaggaacaag ttcttaccaa aatgttgctt tattccttga
    22681 aagaaaaaat aaatgatatt tcctgatccc tcacctttcc attgcgccat tacataaaaa
    22741 acagcacatg tagcaggcac gatagtaata acaggcaaac acacgattac ccagagaata
    22801 cctaataaga tgtaatcaga aaccacaatc aactgtccat aaaatttagt ctcaactatt
    22861 ttcatcttcc tcactctttc attccagaca tagctgttcc ttgaacaaaa tatttctgtg
    22921 caaaaatata aaccactagt aatggcagca ttgaaataac cgttccagcc attacaggtc
    22981 cataaaaaga aatgttctgc cctgtaaata aagctaaccc tgctggtaaa gttctcattt
    23041 ttgaactact tgtcaatatc aatggataaa gcaagtcatt ccaactattc atcagcgtga
    23101 aaatccctaa tgataaaaga gtaggtttgc tcaatggcaa catgattttc aaaaaaatat
    23161 taaactcact taatccatca atgcgtgctg cctcttccaa agaatctggt aaagtaataa
    23221 aaaaggaacg catcatgaag ataccaaatg cagttgtcat acgaggaatg atcaaacctg
    23281 cataagtatc aagcatgcca agcaaattga cttcaataaa tagaggaatc atgaagactt
    23341 ggaatgggat catcattgtc agcaaaacga aatagaacaa aactgatttt cctttgaaac
    23401 gcatacgagc aaatgcatac cccgcaagag aatcaaagaa tacagaagtg atcactgtcc
    23461 ctccagcaaa aatcactgta tttttgatgt aatctaataa agggatcgtt gtccaaacct
    23521 taatatagtt atctagggta tagctatctg caaatattct aggcggaaat gagataatgt
    23581 ctttttcgtg tttaaaagaa gaaataatca gccacaaaaa aggaaacgca ataaccaaca
    23641 atataactag catactaatc gttcgaatga tttttttacc actaactgct tttcgactat
    23701 tcacagtaac tgtaatcgag ctttctggtg ccatacaggt ttccctccta tttcatttgt
    23761 ttttctttcc gtgacatgta cacattagtg gagatagcta tagttgcaac aatgataaac
    23821 aaaaccgaag acagtgctga tgcatagccc aattcataag gtgctgtaaa tcctcgactg
    23881 taaatatatt gaacagctgt ttctgtctta aattgtgggc caccagcggt tgcaacatat
    23941 acttggtcaa acacttgcat cgaaccaatc agattcgtta aaatacaaaa acctaaggag
    24001 ggaacgatct gaggtagcgt aatgttaaaa aactgctgtc gatttgttgc accatccatt
    24061 tgagcagatt catacaaaga gggagagatt ccctgaatag cagtcaagag aatcgtcata
    24121 gtaacaccaa aattcttcca aacagtcatg atggctaccg tcggcatagc aagggttgga
    24181 tcgcgaaaaa attgagggat cggcaagccg acttgtgaaa acaagtaagg aatcgcccct
    24241 acattcggat caagcaacat agaaaaaata atcgcaattg acgtcaatga acaaataact
    24301 ggaatgtaaa atgtcgaacg acaaaaacga ttgaatctgg tatttttaga taatgctact
    24361 gctgctaata aaccaacgat aatttgtgtt ggcgtttcga gtacggtgaa atataaagaa
    24421 tgaatcaacg aattaactgc tcgttcatca ctaaatatac gtgtgaaatt ttcaaaactt
    24481 acaaaatttc ttgatgtaaa gaaaatattc aaatctgtaa aactaatagc aaatgttcct
    24541 aaaagcggta tgacagtaaa taataataaa actaaaaaag atggagttaa aaacaaatac
    24601 gcagcacgat caggacgatt ccaatatttg gtgaatccgt tcatatagtc cacctcatct
    24661 ttcattttga taaaatatac aatcttggtt taaacgaatc tgaataagat atccgaacaa
    24721 tcgctgtgat ttttgcaata acacctatct gcaaatacta ggtcgatcat tcagatatct
    24781 gtatcttgcc aacagtctat cctgtataaa gttctgtact atttttgcag taattgattg
    24841 atggcatcat ctgcatcgtt catgagtgtc tcaggatctt ctcctgctaa taatttttca
    24901 atcagtggat tgatgatatc gttattgatc gcagagattt gggtaaaacc tggtaaatat
    24961 ggctctgcat agtccatttg tttcgataat tcagatacaa taggatcatt ttttacttct
    25021 tcatcttttg ctactgaatc caagtaggca ggaaaaccat tttcgatact ccatttttta
    25081 ccaatttcag ttgtattcca atatttcaaa aactcataga cagcttcttt tttattatta
    25141 tctgtacttg ccggaatggc atatcctacg ccatctaaaa tagctgtttt tttgtctgaa
    25201 tcactccctt gtggtaaggt agtcacatta taatcaatct cgtttgcttt caatccgtta
    25261 tttaaccatg gcccattgat ttcaatggct agttgaccag cattcattaa attgtccatt
    25321 tcggcgccag aaatcgattc tggaccagtc ttgtctacat ggatcatttt ttggatcgct
    25381 tttaacgttt taaggttctc aggagacgca aattcagact tagtataatc atcgttcact
    25441 aatttaccgc catttgctaa catccagtta tataacggtg cagttccatc cttattgaag
    25501 acaaaacctg atacattttt actactatca gttaattttg gtgccatttc tgtcaattct
    25561 tcccaagtag taggtggttg ttcagtatct aatccagctt gtttaaacaa atcattattc
    25621 caatacatat acatgccttg tacctgcatg gggacaaaat actgtttacc ttctacttca
    25681 cctaattcaa tagccgtttc tttaaaatca gatttatcta ctccatcata ttcccagaaa
    25741 tcgtctaatg gttgtaccgc tccgttttct acatactgtg caaaatctgg ataatttaat
    25801 gcaataaagt caggtgcttt tttagaagaa atcgctgtag gaagtttttc attcaaattg
    25861 gcccaagtca tgatatccat ttcaattttg atgtttttat catttgtttt attaaattca
    25921 ttaacgattt cttgcaatat ttcaccatca gatgcagtaa acccattcca aaaagtcaaa
    25981 gtcacttctt tatcagaatc agattctgct gaacttccgc caccgcaagc tcctaaaata
    26041 cctgttgcag ctaccaacaa agagccaatt aaccattttt tacctttcat tttttgttcc
    26101 tccttcatct gataaacgac atctgaccac attccaagat agcggtttca ataaacaatt
    26161 gattttccca tcttcagctt ttacatttgt tgaatgactt ggttttactt gttcactttt
    26221 aggaagatta gttgcttttg tatcaaatcc actcatttca gaaaaatcaa tcactccctc
    26281 taatgataat tcagacaagt gaacagttag attcatttct tcttctccac ggttaactgc
    26341 aaatatgaca agttctttgt catttattac ggctatactt tctaaatacg gcacttcttc
    26401 aaaatcttcc gtcgcatagt ttgctgaacg aatttgtggg cttaacacct ctccttgtcc
    26461 atacacagca gcttgcataa atggataata gatcgtctgt ctccaagctt ctccgtttgt
    26521 ttctgtcctg attggtgcaa taacgtttac caattgagca agacaagcaa tctttactct
    26581 atctgcatgt ttcaataaag tgattaatag acagcctaat agtaatgcat cttcaaaatt
    26641 atagttatct tctagtaaag gaggagcaat ctgccaaggc gctgcttgct catcttgttc
    26701 cgctgaatga taccaaatat tccattcatc aaaggacagg ttgatctgct tcttactatg
    26761 tttcttcgcc ttgactgtat cgcacatcgc tgtcacttca gaaatgaacc gatccatgtc
    26821 taaggaacgt gcaagataat gagaaagatc atttttttgg ttgccataat attgatgcag
    26881 agacaaataa tctatttgat cgtacgcttg atcaagaact gttaactccc aatcaccaaa
    26941 tgtaggcatt tgccgattcg aacttccgca taaaaccacc tcaattgaat catctacgag
    27001 tttcatcgcc ttagccgttt cctctgctaa ccgtccatat tcttccgctg ttttatgtcc
    27061 aatctgccaa ggaccgtcca tttcattccc taaacaccat gtcttaattc caaaagggct
    27121 ctcttgacca ttcgaacgcc ttaaatcact ccaataagtt cctttaggaa aattacaata
    27181 ttcgactaaa ttacgagctg catctactcc acgagtaccc agattgacag ccatattgat
    27241 ttcggccccc acatgctgac accagttata aaattcatga atcccaactt cattcgtttc
    27301 aattgtccgc caagctaaat ccaaccttct aggtctattt gctatgggcc caatactgtc
    27361 ttcccaacga aatcctgaca caaaatttcc accaggatat ctaatcatgg gaatgttcaa
    27421 ttcttttacc aagtgaatga cgtcttggcg aaatccactt ttattagctg atggatgatc
    27481 tggctgataa atcccttcat atactgctcg tcccatatgt tcaataaatg aactgtacaa
    27541 ccgcggatca actttgccta cacgcttgac actactaatc aacattgtgg cttccataac
    27601 tgacctcctt taatttatac atgaataata cacctcgttg aaagcgttgt caataatata
    27661 aatgttaaac ttacttattt tttatcgatt ttaattaacc aaaatattaa taatgattaa
    27721 aacaaataat ttaatataac ttttattaac aaatatgtta atatataaga aaaacctttt
    27781 aagaggtgat atatatgcaa ctacaaataa acgaagaatc cttaccggta tatgaagcat
    27841 tagctagtaa aacaaggatc aagatcatcc agctactttc caaaaaaaag atgaatgtca
    27901 aagatttggc aaaagaactt ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc
    27961 tagaggaagc aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc
    28021 gtgtcgataa aatcgatatt agttttccag aaaaaatatt caatgctttt gatacgaaag
    28081 aaacatcgat acctattggt cactatacaa attatgcaat tgaaccaaca tgcggtttag
    28141 ctacgatcca tgattttatc gggaaagtgg atgaaccacg ctattttatg gatcctcgaa
    28201 ggatggatgc ccgcattctc tggtttacca gcggatttgt cgagtatcag gcacctaatt
    28261 ttttaaatac agaggataca ttggagatgt tggaagtttc tgtggaaatt tcttctgaat
    28321 ttcctttttc aaatgacaat tggccttcag atatcacttt cagcctaaat ggtgtggagc
    28381 taggaacatg gacgagccct ggagattttg ctgatatccg cggtaaatat acgcctgatt
    28441 ggtatccaga taatcttaat caatacggct tgctgaagac gatccgaatt accaaacact
    28501 tgacgaatat gaatggagaa cccttgtcaa acatcacgat caacgacatc cctaaagagc
    28561 aagacacttg gcatttgcga atcgaagtga aagacgatgc caaacatgtg gggggctgta
    28621 ctttatttgg taaaggattc ggtaattacg atcaggatat caaattaaaa gtttactaca
    28681 gctgatattt ttcttttcta ttttataaaa caaaaacagc caatccctat gaagggacgg
    28741 ctgtttttgc tttattgtta tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt
    28801 ttatgttgtg tcttgttcca ccatgcttcg attttttctt gtgcttgagc gggaacttct
    28861 tttccttcta gataatcatc aatttcatta tatgtgacac ctagtgcgac ttcatctgca
    28921 atcattggtt tcccatcttc aagatctgca gtcggaattt ttgtgtataa tttttccggt
    28981 gcattcaatg cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa
    29041 atgtcggctc caccatcacc aaatttcgtg aagaatccag taatgttttc tgcagcatga
    29101 tctgtcccta atacggctcc tgctctttct ccagctacgg catattgcgt aatcatacgt
    29161 tgacgtgctt tgatatttcc tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat
    29221 acttgtactt gtgcatcaac agctggtttg atatttactc gcagactaac atcgggctgg
    29281 ataaagtcaa gagcagcttt tgcatcttct tcatctgctt gttcaccata aggaagacga
    29341 acagcgataa attgatagtt ttcatctttt gtttcttcac gcatttcttc catcgttagt
    29401 tgcgccaaac ggcctgctaa agtagaatct tggccgccgc tgatacctag tacaaaagtt
    29461 ttaagaaacg gatatttttt caaatatgct ttcataaagt caatactttt acgtatttct
    29521 tcttttggat caattgttgg tttgacacct aattcattga taatttctgt ctgcaggctc
    29581 atttattctc ctcctatgct tctgtatgaa gttctgctac gctttttttc attcgttcca
    29641 tgatagccat tttatggttc cagcattcag tagacagatc gactgggtat ttttgcggat
    29701 tcaagtctcg tttatactct tcccatagtg aatctagatt ttcttttgta tattctttga
    29761 tttcgtttag tgcaggtaat tcgtaaactc tttttccttc gataaagata tcttgtaaaa
    29821 ctgggcgagc tgtaaagtct cgaacagttt tattgataaa cgtatgcact ggatggaaca
    29881 tgaagatagc ttcttcttct cgagggtctt catcccataa tgtcacgtag tctccttcag
    29941 atttcccatc aaaatttcga gtgatgcgcc acacttgttt tttccctgga gtagttactt
    30001 tttctgcatt gcttgataac ttgatcgtat ccatcatttt tccttcatca tcttcgatcg
    30061 atactagctt gaatactgca ccaagggctg gctgatcata ggctgtgatc aattttgtcc
    30121 caacacccca tacatcaatt ttagcttttt gcatctttaa attcaaaatc gttgcttcat
    30181 ccaagtcatt cgaagcatag atttttgctt ccgtaaatcc ggctgcatct aattgttccc
    30241 gaacacgctt agatatatag gccatatctc cactgtcgat gcgtacgcct tggaagttga
    30301 ttttgtcacc taattctttc gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat
    30361 cataagtgtc gactaagaac acgcagtctt tatgggtttt agcgtaggcc ataaatgctt
    30421 cataatcatt tccataagat tggactaacg aatgagcatg ggtcccgcta gctggtatcc
    30481 caaatatctt cccagcacgt acattgcttg ttgcatctgc tccgccgata tatgccgctc
    30541 tagttcccca gactgctgca tccagttctt gtgcccgtct agttccaaat tccaatagtg
    30601 gatcttctcc gatgacagat ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat
    30661 tgaccatatt caacagtgct gtttctacta attggcagtg cgctaaaggc ccttccactt
    30721 ggatcaatgg ttcatttgca aaaactagtt ctccttcacg agcagaacga actgttgctt
    30781 taaattcaaa attttgtaaa tactccaaaa aaccttcagg atagacttct aatccacgca
    30841 agtaagcaat atcggaatca ttgaacgtca gattctctaa ataattgact aaccgttcca
    30901 atcctgcaaa aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa
    30961 cagcatgtaa atcagctcga cctaattccc agtatgtctg catcatattg atttgatata
    31021 gatccgtatg cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg tattactcct
    31081 cttggcaagt tttcatttcc tacccattat aacaaaaaaa attaatagga aaagaaatct
    31141 tccatttttt ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa tgaactccat
    31201 ctgctaaaac tatcctttct tattggtatt taacctaaac gagaaagaat acttatttta
    31261 ttcaatgtat ccgagacacg aagaaattag cgttttctat ttttcttact tgataaaata
    31321 agtagatttt cctactacaa tcgtagccgt taagataccg atgattgaac aaatataacc
    31381 aaatgtctct acaacataat caatggcaaa agtttttgaa gcaataatac gtctttcctt
    31441 ttttgcttct tatccccaaa gaaacggata taacttatta tgtattgaat ttttttggaa
    31501 aggcgaatta tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg
    31561 tttttaaaaa gatacaaagt aagaaagcag ctgtcaatag aatggaaaca aaaatggcga
    31621 gacatcaatc agtctcgcct tgttttt
//
LOCUS       DAFCNJ010000031        29983 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000031 DAFCNJ010000000
VERSION     DAFCNJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.032"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            139..477
                     /locus_tag="LNE35_001657"
     CDS             139..477
                     /locus_tag="LNE35_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910819.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="LNE35_001658"
     CDS             complement(682..1257)
                     /locus_tag="LNE35_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK6910820.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="LNE35_001659"
     CDS             complement(1989..2570)
                     /locus_tag="LNE35_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6910821.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="LNE35_001660"
     CDS             complement(2753..3379)
                     /locus_tag="LNE35_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6910822.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="LNE35_001661"
     CDS             complement(3401..4732)
                     /locus_tag="LNE35_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBK6910823.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="LNE35_001662"
     CDS             complement(4887..5288)
                     /locus_tag="LNE35_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK6910824.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="LNE35_001663"
     CDS             complement(5374..5580)
                     /locus_tag="LNE35_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910825.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="LNE35_001664"
     CDS             complement(5675..7177)
                     /locus_tag="LNE35_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBK6910826.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="LNE35_001665"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="LNE35_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK6910827.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="LNE35_001666"
     CDS             complement(7748..8365)
                     /locus_tag="LNE35_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBK6910828.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="LNE35_001667"
     CDS             8586..9959
                     /locus_tag="LNE35_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6910829.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="LNE35_001668"
     CDS             10034..11146
                     /locus_tag="LNE35_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBK6910830.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="LNE35_001669"
     CDS             complement(11218..12855)
                     /locus_tag="LNE35_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBK6910831.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="LNE35_001670"
     CDS             complement(13023..13631)
                     /locus_tag="LNE35_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6910832.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14015..15166)
                     /gene="queG"
                     /locus_tag="LNE35_001671"
     CDS             complement(14015..15166)
                     /gene="queG"
                     /locus_tag="LNE35_001671"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBK6910833.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15185..15937)
                     /locus_tag="LNE35_001672"
     CDS             complement(15185..15937)
                     /locus_tag="LNE35_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910834.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16078..17028)
                     /locus_tag="LNE35_001673"
     CDS             complement(16078..17028)
                     /locus_tag="LNE35_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBK6910835.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17029..17868)
                     /locus_tag="LNE35_001674"
     CDS             complement(17029..17868)
                     /locus_tag="LNE35_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK6910836.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17865..18611)
                     /locus_tag="LNE35_001675"
     CDS             complement(17865..18611)
                     /locus_tag="LNE35_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK6910837.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18849..20219
                     /locus_tag="LNE35_001676"
     CDS             18849..20219
                     /locus_tag="LNE35_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6910838.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20274..21170)
                     /locus_tag="LNE35_001677"
     CDS             complement(20274..21170)
                     /locus_tag="LNE35_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6910839.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21284..21454)
                     /locus_tag="LNE35_001678"
     CDS             complement(21284..21454)
                     /locus_tag="LNE35_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910840.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(21451..22128)
                     /locus_tag="LNE35_001679"
     CDS             complement(21451..22128)
                     /locus_tag="LNE35_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK6910841.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22219..23124)
                     /locus_tag="LNE35_001680"
     CDS             complement(22219..23124)
                     /locus_tag="LNE35_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK6910842.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23299..24753
                     /locus_tag="LNE35_001681"
     CDS             23299..24753
                     /locus_tag="LNE35_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6910843.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            24769..25800
                     /locus_tag="LNE35_001682"
     CDS             24769..25800
                     /locus_tag="LNE35_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK6910844.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25807..26994
                     /locus_tag="LNE35_001683"
     CDS             25807..26994
                     /locus_tag="LNE35_001683"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBK6910845.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27150..28532
                     /locus_tag="LNE35_001684"
     CDS             27150..28532
                     /locus_tag="LNE35_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK6910846.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28504..28890
                     /locus_tag="LNE35_001685"
     CDS             28504..28890
                     /locus_tag="LNE35_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910847.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29090..29758)
                     /locus_tag="LNE35_001686"
     CDS             complement(29090..29758)
                     /locus_tag="LNE35_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBK6910848.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8404 a         6141 c         5483 g         9955 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcattta gaaatatttc ataggtaaaa gaaacatgac atttaatgac aaaataacga
    13741 attatttaat atttgtaaca tttaaacaaa tatttttttg atacaaaacc ataaaagcct
    13801 tgttgtataa gcaaagtaat cgtttttcta tgttttttta tttttgaatg aaaaacgcag
    13861 attctgtaac attcactgca ataatccata aaaaaaagca aaaaaatttt tgatttttat
    13921 caaattctct taaaaaaatg taaaaaatac aaaaaaacca gtaattgtta caacaattac
    13981 tggacaaaaa ctttgaataa tgttaactat ttttttattt taactttctc cgcaataatt
    14041 tctgggcatg ttccaattcg tttataacga cttcttctgt ttctttagct aatatttcat
    14101 ctaatacatc aagccatttt tccggttctt ttgttcctag acgtccaata gcccaagctg
    14161 ctgttcctct gatgacaggg cggacatctg ttaaacattc tgaaatctca ggcaatgccg
    14221 ttcgatcacc tagattagct aaagcaatca atgcgtttct ctgcaaaggt ttcttcccac
    14281 gccatgaacc agctaaatgc ccaaattgct gtttgaagtc tttattagac atcgaaagca
    14341 ttggctttaa ttttggatat acttcgtcta cttttggttc catctcttca tgaaaatgaa
    14401 aatcttttcc ttgattgtaa gggcagacta actggcagat atcacatcca taaatgacat
    14461 ttcgcatttt tttacgatat tcttccggca tcatcccttt tgtctgagtt tgataagaca
    14521 aacatcgctg ggcattcatt cttccgtcac caagcaatgc ttttgtcgga caagcagtca
    14581 cgcagcgcat acagtcaccg caaccaaatt ctcctggttt atcaggctca aatacaatat
    14641 tagtaacgat ctcaccaaga tagacaaatg aaccgaactc ttcagtgatc aacaatccgt
    14701 ttcgtccaat aaatcctaaa ccagctcgct gtgctacagc gacatcgatc agttctccag
    14761 tatctacttg cggagctaga cgccattcct cttctttttg ccatttttct gctcttgttt
    14821 ggatgaattc gatcaatcgg ttcaaacggt ctctgagtat atcatggtaa tcgatccccc
    14881 aagaagcacg agcaaactgc ccgcgttttt catccctagg cacttcttca tggatcctag
    14941 taggatacgc aagtgcaatg gaaatgatcg atttcggctg atcaaatgtc atttctggat
    15001 aaagacgctc atcgatattt ggatgttcaa agccagaagt atggccagct gctttttgtt
    15061 cttccaacga ttcttttaat gaatcaaaag gttccgccgt ggtgaacccg attttgtcaa
    15121 ttcctaatcg tttgctttct tgtatgattt cttcttttaa tgtcatggtc ttcctcgctt
    15181 gctgttaacg gctgtataac ccataatata gtttttcttt tttcagctgg acaaactgga
    15241 tctgctgatc ggcaatcttc ttttcttcca tccatttttt tcttgcagca aatacttccc
    15301 aataatttgt tgaaattaat tttcttttgt tatctaatgc attaaaaaaa ttcaatgaaa
    15361 ctggttttgt acttaacagt tcatggagcc ttacctcttc ccatggcgaa aactcagaca
    15421 gatcgcggga atacaatact tcttcataga gatttttttg ctgtgtgtac gtgaatgttt
    15481 gcgggatttt cccaggccta gcaatttcta ctagacgatt ataaaacata ttcattaatt
    15541 tgctgttggc aataaattga cttttataat aggccgactg gatctttttg attactcctt
    15601 ctgctttgat gactgaaaat aatgcaggcc atgctttgac ggttatttcg tcgttttgtt
    15661 taactaatac gccaggtgtg aagccgactg gagaaaggaa cacaaaataa ccgataccgg
    15721 tattttcatc taaaggctga aattcatcaa acaaccattt gcctatgtac agctcttcat
    15781 ctggttctgt tgtctcattt tgatttgcta tgaataattt agctaattct gcttcaagaa
    15841 attgaaggtc ttgatccttg tcggtatttt cctgctggct gatcaatcga aagacttctt
    15901 ctttaatcgt atatttatct ttacgtgtaa aattcatagc cacaccttcc ttccttactc
    15961 taactataac ggaatttctt caaaaaaggt atctgtacga cgaaaaacag cataattttt
    16021 tttcatatct cttataaaaa atgcaaggtc tgctcctcaa gctagacctt gcacttttca
    16081 gctttctttt gtttgtgaca aaccttcatg gatcgtttca atattccatt tcatcatctg
    16141 atagtaagaa tctccttgtt ctccttcttt tgctatggaa tcagtgaaca gtttatcata
    16201 gattttcaaa cccgtttctt ttgctacccg ttccatgctt cttgagtcta cactggtttc
    16261 cacgaataaa accggtactt cttttgctct tatctgatca atgatcgctt tcatttgatc
    16321 aggcgtacct tgactttctg tattgatctc ccatatataa gctgctggca aatcatatgc
    16381 tcgtgaaaaa tacttgaatg ccccttcact tgttactaat aattttttgt tctcttcgat
    16441 agaagcaaaa ctttccttcg cttgtgtatc taattctttt agtttttcca catatgcttc
    16501 tgcatttttt ttatagagtg tcgcattttc tgcatctttt tcagagaata tacgtgcgat
    16561 ttcctccaca tattttattc cgttagatag gtctagccaa gcatggggat ctgcttttgt
    16621 atgttcttca ccgctagtta aataaagagg ttctacattt ttgctaactg caaaataatc
    16681 ttttccttct tcttttttag ccgtttccat caagttatcg aaccagctgt tacctgtttc
    16741 aagattcaaa ccgttgtata aaataacatc tgcatcgctt gcctttttga tgtcttctgg
    16801 tagtacttca tattcatgcg gatctgttcc gactggtacg atactgtgga tatctatttg
    16861 atctgttccg acctcttttg ccatgtccgc caagatcgaa ttggtagcca cgactgacaa
    16921 cttttcttta cttccttcat ccgcttggtc agaagcctgc tttccgcaac cggctaatac
    16981 caagaaacta actgccaaga ttaaaaagag tgattttttt gttttcatct atctgcttcc
    17041 tttctgaaat aaaattcctt ttgttggaga aaataaaaaa gcaaggataa aaaatgcggt
    17101 acatgctaac acgatcgtag ctcctgatgg ccaattgtaa ctataactga aaaacacacc
    17161 gacaactgca cttagcatac cgattcctgc agctgtaatg atcattttca gtaaatggtt
    17221 cgttagcaag taagccgtgg cggctggtgt gatcaacatg gcaatcacca agattgttcc
    17281 tactgtctgt aaactgacta cagccactaa tgtcaaaaag aacatcaaaa gataatggat
    17341 cagcgatgtg ttcagcccat aagcttttgc cattgtcgga tcgaatgatg tgatcttcaa
    17401 ttctttataa aagaaaaaga caaatatcaa gacgattcca ctaacgagtg ctgtcagcaa
    17461 caaatcgctt tccctaacag ctaagacatt cccaaataaa atatgataca aatctgtcgc
    17521 actctgggca tatgaaatca agataatccc taatgcaaaa aaggagctaa agacaattcc
    17581 aatcgctgta tcatttttca atcggctccg ctgagtcacg aaaccaatca ctgctgctgt
    17641 tagcatccca aaagcagtgg ctccaaagat gtaactaaaa ccaaacatat aagaaatggc
    17701 tacacctgga agaactgcgt gagagatagc gtctcccatc aaagacatcc ctcgtaaaat
    17761 aataaaagaa ccgatgactc cagaagcaag tcctacaagc atggaagtga ccaaagcgta
    17821 ttgcaggaat tgataattca ttaatccatc aataaaggat tggatcattt tttctctcct
    17881 ccttttacta aatgtctgat gatctcacca taggccaatt gaaggttctt cgatgtaaag
    17941 acttcttgta ctggaccgga agcgatcagc tgtttattta aaagaataac ctcatcgaaa
    18001 tattcttcta ctttatgaag atcatgatgg acgatcaaaa tcgtttttcc tgaattcttc
    18061 aattcctgca agatgtcaaa gatctttcgt tcacttaacg catcaatccc tacgaatggt
    18121 tcatctaaaa agatccattc tgctccttgg gctagagctc ttgcaataaa aactctctga
    18181 agctgtccac ccgatagttc gctgatctgt cttttggcat attcttccat ccctactttt
    18241 ttcaaagctt gtcttgcacg ttctttttct tttttcccag gtctttgtcc aattcgtaaa
    18301 gatggatatg ttcctaaaag tacaacgcct aatacatcaa ttggaaagga aagatccaat
    18361 tcacttcgct gttccacgta ggctattttt ttacggactg agtttaccgg ctgtccagaa
    18421 aaggtgactt taccagtagt agctggtatc aactctagca aagatttcat aaatgttgat
    18481 tttcctgcac cgtttggtcc gataataccg gtaatttttt gtggatttat cgtcaagttg
    18541 atagttttca aaacagtttt cccttgataa gcggctgaaa ggtcttgaat tacaatggcc
    18601 gtacgttcca aatcttacac tcctttttag gtttacctaa ctttttattt aaatatagtt
    18661 taactaaaat ggaaagcatt ttcaagtaga atgatgaaaa agtttttttc gaattttcct
    18721 cgcattttat gaaaattttc tgtagaatca catcaagagt tcaataaggc taacctaata
    18781 atgaaaaaca tgtgttgaaa cgtttgatta ttttttcttg ttgactcaac aacgaaagga
    18841 gtttttgtat ggaagaagaa aagttaactc gaagtcttag tgcccgtcat atccaaatga
    18901 tcgcactagg tggaacgatc ggtgtcggtc tgtttatggg tgcctcctca accatacgtt
    18961 ggacagggcc ctcagtgatg ggagcctatg cgattgcagg attctttttg tatttgatta
    19021 tgcgtgcttt aggcgaaatg ctttacgttg atccttcgac tggttctttt gcaaattacg
    19081 cgagcgagta catccatcct gtggctggct acttgaccgc ttggagtaat atttttcaat
    19141 atattgtcgt aggaataagt gaagtgattg ctgtaggatc ttatatgaat tattggtggc
    19201 ctgatttgcc ggcaattatt cccggaatca ttgtcgtctt gtttcttatg ctggcaaatc
    19261 ttatttctgt caaggcgttt ggcgaattag aattttggtt ttcgatcatc aaagtcataa
    19321 cgatcatttt gatgatcatt gcaggtcttg gggtaatctt atttggattt ggtaatcatg
    19381 gtcaggcagt agggatttca aatctttgga aaaatggtgg attcttcact ggaggcgtca
    19441 aaggattttt ctttgcatta tcgatcgttg ttgcttccta tcaaggaatc gaactgatcg
    19501 gaatgactgc tggtgaagca gagaatccta aaaagacgat cattgaagct gtccagtcca
    19561 ctatcggtcg aatcttgatt ttttatattg gtgctatttt tatcattgtg agtatctatc
    19621 cgtggaatca attgagtgag attggttcac cttttgtaca aactttttct aaagtcggca
    19681 ttacatttgc tgcaggactg attaattttg tcgtgattac agcggcatta tcaggttgca
    19741 attcagggat tttcagcgca agtcgtatgg tctatacact tgccataaat ggaaagatgt
    19801 ctaaaaaatt tctgaaacta tctcgtcacg gtgcgccttt ttatcctgtc gtagcaatct
    19861 caggtggaat cttaattggt ctgatcttaa attatcttct accaatgttc atcaaccagt
    19921 cagaaaatat tttcgttttc gtatacagct caagtatttt gcctggtatg gttccttggg
    19981 tagtgatttt gatcagcgag attcgcttca aaaagaagca cgcagataaa atgcatgatc
    20041 atccttttaa aatgccacta tctccttata cgaattatgt gacacttgct tttttagcta
    20101 tcgtattgat tttcatgttt atcaatcctg aaacgagaat ctcattgatt attggactcg
    20161 ttttcttgct ttacatgacg atccattact ttgtacgcga aaaaagaacc ggaaaataaa
    20221 aaaagaacga agcttcacct tagtggaagc ttcgttcttt tttattgctt ttatcaattc
    20281 aattttagcg tctcaccggg agtcaggaaa taattctcca tgctcatccc attcagcgac
    20341 aagatcgttt ctactggtac gccagttcgt tcggctattt gatttggacc ttctccttcc
    20401 aaaactacgg ttgtcgcagc ctgtgaagtc gcgctgttgg tattagtttc agaggaatct
    20461 gttgtgtctt gtgttgttcg agcttgcatt tcagaagctt ctaccgactc tgctgtctca
    20521 gaatcagaag atgttgtgtc ttgtgtgctt gttgactctg cttgaagaga ggcgctagtc
    20581 tgcaaataaa tatcggaaat cttagcgaca tgattcgtat aattagccga taattgattg
    20641 atcaaagtat ctaagtctac accgtcttta ttttctgctg cctgatacgc tgcacgtaat
    20701 tttctgatcc cttcgtcgga gattgcttga agctcacgtg ctttttggtt agcaactgct
    20761 gataaaccag ctattccctc ttgatttccc tgaatttctg tctggtattc ttcaatcaat
    20821 gcaggcgtct tttcttcgat ctttttcgaa taatcattta cgattgcttg aagttgctct
    20881 ggcaaaccat ttgctgtttt ttcttcagaa gtttgtgtct cttttgtttt agttgatgac
    20941 tcacttgttt gtttcgtctc tgttttcttt gtcgttgttt ccgttatggt cgtacttttc
    21001 gcttctgacg aattttttcc atgaaaatat agacccgcaa ctcctcctgc tatgatcaaa
    21061 aacacggcaa ctgctgcccc aatcatgatc atccgcttac gagctgcctg tttttgctgt
    21121 cttcttcggg tcatccgaac attttctccc cactcatttt catcatacat ttttttcacc
    21181 accaattttt atgtataata gcgttatcaa acgcttcata ttcttattgt agcaaataat
    21241 tgtcgctgtg cacattttta tctttgttat ttataagaaa atttcaccag ttgagatgat
    21301 tccaagattg tccgtcgatt tctccttgca gctgcattgt tcgttcgtct atctcagcca
    21361 tcagtttctt tatttcatat aagagcgcac ggtcttgata ttctgatttt tcgcttaatt
    21421 catctaattg ttggatcagc caatcatttg tcataatgaa gttcctttca tttcttgcag
    21481 tgtaaaggtc aagtcttcga tctgctcttt tgcctcagtc agtgcttcta cgatatatgc
    21541 ttgtttttca gattcttctt cctgcatttt ctgattttcc tgtactcttt gaataaacca
    21601 gtcaatccgc tgagtcagcg atggtttgcc ttccaatgag agatactgct gataagcaat
    21661 tagcttctcc caacgttttt cctgggataa atacataaat tgtgctaaaa cgaatggcgg
    21721 aagataggtc atatggaatt tatttgcgat tcgctgatac ggcctcaaaa attcggaaat
    21781 cgtatattct tcttttcctc cagcttggta ttctttttcc gccacacctg ttgtcaatac
    21841 gattcctagt tctttccctt ttaatacatg tgctgctcgt tccaagacat tatcttgcca
    21901 aatttttaaa tgagcaggag aactatacca gtaaaacgga aactggaaaa tgatccgatc
    21961 atgctgggcc aagagctgta gttcagtctc tatatctatt tttccgtctg gataacttga
    22021 ttccaaatga tgccaagtag ctggtaaacc agcagaagct tctttgaaaa attgctgatt
    22081 taaagattct tcgatcgttg gatggctaat gatgataagt gtacgcatcc cttttcctcc
    22141 tttttagtct tcgctttaca gtgtagcata accgccacaa gtttcttttg ccgatgcttg
    22201 tcattttaac cagactaatc aggttgtcca agaaactgcg gtttgttttt ccaactgttt
    22261 tttcaaggta tccaaagcaa tattcttaaa attttcgttt ttcatacgtt tacgatagtt
    22321 cgtagccgtt tctccggtac tctttttgaa cattcggcta aatacgacta gactattgaa
    22381 ccctacttga taggcaatac tttccacgga ttcatccgtc agagaaagaa gaaacgcccc
    22441 tcgattgatc cgtaccccga tcaaatattc cttgatactg gtacccatat gatttttgaa
    22501 tactcgcgac aaatggctcc gattgaccgc taaatgctgg gcgatttcct caacagacaa
    22561 tttttcagca taatgtgcct cgatgtattt gactgtttca acagctaaag aggaatgaac
    22621 cttttgtaca ccaccgctga cctttccgcc atcttttaaa aggactgata aaaaacggta
    22681 ggttaatgct gttaactgta attcattttc caaggtttct tccgtatctt tcatgcattc
    22741 taaaataatg tcgatatatt tttgtatgtc agaagagacc atcgtgtatt ggtcttctct
    22801 gaaaagcgac tgccgaatga tttcttctgc agcactccca ccaaatgaaa gccagcaata
    22861 catccacgga tcttctccat ctgataagta aaacgtcgag tctcctggac gaatcaagaa
    22921 cagatcacct tttttcaatt ggaattgttg atttttcaca tgataagatc ctttgccttc
    22981 catcacgaca tgcaggatgt aatcattgcg aatagcagga ccaaaagaat gaaacggcat
    23041 cgttttagag aaaccacaaa aatcaaaata caattctttt tctacttgtt gatgttcctt
    23101 ttttaaaaaa atcgattgct ccacgtctat ccctccttac tcctttagta taatgcaaca
    23161 aaaagttaaa taaaagtcac agaaatatat taaaaaggaa gcgatttctt gttaagttta
    23221 agtcattaaa agatctttgt tattcgttgt aaccggttta cttagtatta tatattgaac
    23281 aggagaggaa tacagattat gaaaagcaaa atgatccaac gtgtgtccta cgctttaggc
    23341 gcttttggac acgacacatt ctatgttaca ttaagcactt attttatggt attcgtgaca
    23401 agccagatgt tcgcaggaac agataaggca acgaatgcaa aaatgattgc tacagttaca
    23461 agcttagttg taggtatccg cttagttgaa attatttttg atcctttgat tggtggcatc
    23521 atcgataata ctcgtacaaa atacggtaaa ttcaaaccat ggttagttat cggcggcgtt
    23581 gttagttccg tactcttggt agcaatcttt accaatttct ttggcttagc tactcataat
    23641 acgactttat ttatcatatt atttgcaatc gttttcatct tattggatag tttttattca
    23701 tttaaggata tcgctttttg gtcaatgatt ccagctttga gtacggaaac aaaggaacgt
    23761 gaaaaatttg ctacgtttgc tcgtttcggc tcttctctag gtgcaaatgg tactactcta
    23821 gtcgttgtcc ctatcgttac tttctttacc tatcttgtta ctcataaaca tattgaaggc
    23881 gcttcaggct ggttctggtt cggtgtgatt gctgctgtta tttcaggcgg tactgcgatt
    23941 atcactgcct tgggcacaaa agaagtcgat actgcgatcc gtcagcaaaa tgaaaaagct
    24001 gggatcaaag atgtgttcaa agcagttgct caaaatgatc agttgatgtg gcttgccctt
    24061 gcttatatcg cttatgcgat tgccaatgta gcaacaactg ccgtattatt ctacttcttc
    24121 aaatatgtac ttggccaacc aactgaattt tggatcgttg gtctagttgc tgccttactt
    24181 ggtttagtag ctgttccact attcccaatg cttacacgcg tcatctctcg tcgctacgtt
    24241 taccttggag ggatcgcaat gatgatttct tcttactttt tctttacgat cgcaggttca
    24301 agtttgccaa tcgttatcat cgggctagta ttgttctact tccctcaaca attgattttc
    24361 ttatccgctt tgatgacgat cacagactct gttgaatatg gtcaatggaa aaatggtatc
    24421 cgtaacgaag ctgttactct atcaattcgc cctttactag ataaaatcgc tggtgctttg
    24481 tctaacggga tcgtagggtt tgtcgctatc gcttctggga tgactggtaa tgctactgct
    24541 gctgatatca ctgctcatgg cgtaacgact ttcaaagctt atgcattcta tgctcctgct
    24601 gctttgatga ttttctccgc tattattttt gcttggaaga ttacattgac agagaaaaaa
    24661 catgcacaaa tcgttgagga attggaaaac aaattggctc cagctgatac aatggaagat
    24721 gaacttcacg atgcagtagt tccttcccat taagaaagga tcttgaaaat gaagattaca
    24781 gaaaaagatt ttggccaagg ttatcacttg ataaccttgg agaacaaaaa caagctagca
    24841 ctttctatct ctgatttagg tgctagaatc gtcagtttaa aatcaaatga tcgagaatta
    24901 gttttgggat ttgacaccgc tgaagaatat atcgaaaaag atccgtatat cggtgcttct
    24961 atcggtcgca ccgcagggag aatcgaaaat ggccgctttt ctctcaatgg aaaaacttac
    25021 caattagcca cagatccaaa aactggacat aatctgcatg gtggtgctcc tggatttgaa
    25081 ttaaaaaaat ggtcttatgt cattttgaat ggcgaaaatg aagcttctgt catttttact
    25141 actacaagtc ccgacggaga acatggtttt ccaggtacaa tggatgtgga aatccgctat
    25201 actttgacaa aagataatat ctggcgagtg acctctcgcg gcaccagtga ccaagatact
    25261 ttattcaatc cgacaaatca tgtttacttc aacttgacgg gagatgcttc tcagtcaatc
    25321 gatcaacatg agctttggct caatagcgaa gcttatgctc ctttgcgaac agatagtatt
    25381 cctatagggg taaaagaaaa tgctgcagga agtgctttcg attttcaaat acctaaaaaa
    25441 ttagcgtccg tttttgctag tgatttggat caaaaaaatc ttgttgacgg aatcgatcat
    25501 ccatttttct taaaagaaac cggcttgggt aaagaagccg ctcgcttgac tagccccgat
    25561 aaaagaatcc aagtaggtat tgctacagat gcttctagtg tggttatttt cactgcaaat
    25621 ttcggcacag aaacaccaga aatgcgcaac cgaaaactgg cacatcacgg tggcatcaca
    25681 tttgagactc aaacagctcc aggtgcagag cgcttctcag catttggttc gattcattta
    25741 aaagctggat cagtatttga aacagtgaca gaattcaaaa tcaaaacaag aaaggaataa
    25801 ctcatcatgg aaatcaaaag aagcttaaac acaaaatttg ccgaaacatt cggacctaaa
    25861 aaaactacac aatacttctc accaggacgc atcaatttga ttggtgaaca cacagattac
    25921 aatggaggcc atgtcttccc cgcttccatc acttatggaa cgcaaggtgt agcagcacct
    25981 cgagacgata ataaaatatt agtttattcc acaaactttg acgatatcgg tgtcattagt
    26041 tttactttag atgagctaga atatgataaa aaagcaaact gggcaaatta cgtaaaagga
    26101 atggtcctta aattaaaaga agctggacac tcaatcgatc atgggtttga gttgttagtg
    26161 gaaggaacaa tccctaatgg tgcaggactt tccagtagcg cttcattaga attattagta
    26221 ggtgttgtat tagaagatct gtttgattta aaaacagatc gcttggaact agtccaaaca
    26281 ggtaaaaaag tagaaaatga attcatcggt gtcaattcag gtatcatgga tcagtttgcc
    26341 atcggcttcg gtgagctaga tcatgcaatc ttattagata caaatacgct gaaatacgaa
    26401 atggttccag tgaaactaga tggttatgca atcgtcatca tgaatacgaa taagcggcgg
    26461 gaattggcag attcaaaata caatgaacgc cgtagcgaat gcgaagaagc cttggcacgt
    26521 ttgcaaaaaa ctttgcctat ctcttcttta ggagacttag atgaagaaga atttgaatca
    26581 actattgacc aaatcggcga tgatacattg atacgcagag ctcgccatgc cgtttctgaa
    26641 aatcagcgga ctttgaaggc aaaagctgaa ttagaagctg ggaacttaga agcatttggt
    26701 caactgttaa atgattctca ccactctctt cgctatgatt atgaagtaac tgggatcgaa
    26761 ttagatacac tagtagatgc tgctcaaaaa caagaaggtg tcttaggtgc acgaatgaca
    26821 ggagctggat ttggtggctg tgcgattgct cttgtcaaag aagaaaatat acctgccttc
    26881 aaaaacaatg tctatgatga atacatgaaa gttgtcggct atgctccgga attttatgtt
    26941 gcacacattg gaaatgggac aacaaagatc gatccagaag ctgttcatgt ttgataaaaa
    27001 aatcaacgat ctcagctaaa aacaaataga taaaaataac ctctcagcaa tttccaaatg
    27061 tgagcctttt gctcgtattt gaaaattgct gttttttatt gacacggaaa gagaacgtgt
    27121 gttctaattg tgttgaagag gtgattgtta tgtgggcgaa tcaaaatccg gtatttgttt
    27181 atgaaaaaga accttcaaga gatattttat gtattgactg caaatctttt tacgcctccg
    27241 tagagtgcgt tgaaaaggat cttgatcccc ttacaactaa attagtagtc atgtcctatc
    27301 cgtccgattc gacagaaaca cggggtagcg gattaatctt ggcttccagt cctgcagcaa
    27361 aaaaagctta cggcatcacc aatatcagcc gtgccagaga tcttcccttc ccctatccaa
    27421 gtgatttata tattgttgcc ccaaggatgg cctattatat ggaaaagaac agagaaatca
    27481 attctatcta taaaaaatat gcagacgaag cgaaccatca tgtctattcc gtcgatgaaa
    27541 gcttccttga tgtaacaaat agcatggcac tctttggtaa aacaaatgcg tacgacatgg
    27601 ctaaactgat ccataaagat gtgtttgagc agaccggaat ctacacgact ataggaattg
    27661 gcgataaccc gctacttgct aaactagcct tagacaatgg ctcaaaagac ggaaagcaaa
    27721 tgtttgctga atggcgttat gaagatgtac cagaaaagtt atggaaaatc tcagatatca
    27781 ctgacttttg gggaatcggc aaacgaacag caaaacgtct gaaacaaatg ggtatacatt
    27841 ctgtttatga tttagctcac gcggattatt atcacttgaa aagcaagcta ggggtcatcg
    27901 gtactcagct ttatgcccat gcttggggca tcgatcgaag ttttttggga caagaatata
    27961 caccaaaatc aaaaagtatc ggaaatagtc aaatcctaga tagagattat acaagacgag
    28021 aagaaatcga gatcgttatc aaagaaatgg ccgaccaagt cggcactcgc ttgagacgat
    28081 ctgcagcaaa aacacaagta gtcagcttat gggtaggata ctctttaggc tatcttgatc
    28141 caaatggcaa gaccgggttt caccagcaaa tgaaaatcga agcgacaaat gctagcagag
    28201 atctagcaga tgctttattg accattttcc accagcatta ccactaccag gatattcggc
    28261 gtgtaggcgt gaactgttcc gacctcgtat acgcgacagg tttacagctt aacctttttg
    28321 aagatcctga aaagcagata aatgaagcga aagttgattt tgttgtcgat acgattagaa
    28381 aaaaatacgg ctttcaatca atcatccatg cgcatagtct cttgcaagga ggacgcgcca
    28441 tcgcacgtag ttctctcgtt ggcgggcatg caggtggtat gagtggatta gaaggtggaa
    28501 aagatgagac ggacagcaaa aaaattcatt gactataacg agtatcgaga tcggccattt
    28561 ggattaaaat gggggactgc ttttgcgatg gaagaactag tcaacggtat caaaaaaaat
    28621 gaagaagaag cacgcaaaca gcctcttcct aaacaacaaa tgacattgca agaaatcgat
    28681 agtatactga cagaagcctt tttatctcgt aaagcagtaa tcattcagct caatttaaaa
    28741 gatgaatttg gaagactctt ggatgaggtc gaagggcact tcattggtga agcctatgaa
    28801 gattacttta tgattgacca tcagccgata ttgtgggaag acgtacggta tattgagtta
    28861 aaggaaacaa aaaaatggtt tgtgacatga gaggttgtga cagaagtgat caacttcaaa
    28921 aaataagaaa ggatttttcg aaaattgctt cttaaatttt tgtggaattt tggcttattt
    28981 ttcgaagaag caacttctgg aacaacgtat attagatatc agaggttgtg aaaaagtcgc
    29041 ttagttttga gtaacaagcc aaggcgctga tttcacaacc tctgtctatc tatccatcta
    29101 ttcggtaaga ttcatgcatg acgtcttctt tatttagtat gtagagccct ttttcttctg
    29161 accattcaac ataaatcagc tgttttacat ctgcatagcc ttctttcttc aagttctcca
    29221 taagccatgc ttcagtcaat tgattggact gaagcacttt atgctgaatt tgcccttcat
    29281 ctacaagcag gatcgataaa gctttgtctt catctgattt tttcaataca ctcatttgac
    29341 cagcattctc taggataaca tgtttggcat tttcaagaga aaagcattct tgctgtctta
    29401 agattgctcg caactgctcc atctcgatat ggttatttgt caactctgtc atgttgatga
    29461 cgccgtcttt gattagaaca gatggctcgc ctttgaccca acgattgacc ttttcatttt
    29521 tttggacgat acgttcgata ccataaatca ttactgccca aagcgcaatg gcaaatagga
    29581 catgccctac atggacattg tcatcatagg ttgattcttc taagatccca cctaaaacaa
    29641 gcgtatagac taaatcaaac ggtgtgattt ctgacatgga tttcttccca agcaaacgta
    29701 ccactaataa taaaccgatc aacccgaaca ccagtttcat tgtaattgaa aaaatcattt
    29761 tttctccccc taactatctg ttaattaaat tatatatgct ttctcacgaa cacgcatgga
    29821 atatacaagg aagaaatgta tccttttact aaaaaaagga ctgtgacact gtagtgaccc
    29881 ccaaaagtta gacttttttg gagtggagaa atccactcct tttttttatg ctctaaaatg
    29941 atatttttac acaaccgtta cggttgtttt acgaaattgg acg
//
LOCUS       DAFCNJ010000032        28306 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000032 DAFCNJ010000000
VERSION     DAFCNJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.033"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            8..81
                     /locus_tag="LNE35_001687"
     tRNA            8..81
                     /locus_tag="LNE35_001687"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="LNE35_001688"
     tRNA            101..173
                     /locus_tag="LNE35_001688"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="LNE35_001689"
     tRNA            201..274
                     /locus_tag="LNE35_001689"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="LNE35_001690"
     tRNA            288..361
                     /locus_tag="LNE35_001690"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="LNE35_001691"
     tRNA            385..474
                     /locus_tag="LNE35_001691"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="LNE35_001692"
     tRNA            487..560
                     /locus_tag="LNE35_001692"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="LNE35_001693"
     tRNA            579..654
                     /locus_tag="LNE35_001693"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="LNE35_001694"
     tRNA            656..726
                     /locus_tag="LNE35_001694"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="LNE35_001695"
     tRNA            740..813
                     /locus_tag="LNE35_001695"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="LNE35_001696"
     tRNA            838..925
                     /locus_tag="LNE35_001696"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="LNE35_001697"
     tRNA            947..1018
                     /locus_tag="LNE35_001697"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="LNE35_001698"
     tRNA            1041..1130
                     /locus_tag="LNE35_001698"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="LNE35_001699"
     tRNA            1143..1216
                     /locus_tag="LNE35_001699"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="LNE35_001700"
     tRNA            1235..1307
                     /locus_tag="LNE35_001700"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="LNE35_001701"
     tRNA            1311..1383
                     /locus_tag="LNE35_001701"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="LNE35_001702"
     tRNA            1388..1458
                     /locus_tag="LNE35_001702"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="LNE35_001703"
     tRNA            1471..1544
                     /locus_tag="LNE35_001703"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="LNE35_001704"
     tRNA            1556..1627
                     /locus_tag="LNE35_001704"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /locus_tag="LNE35_001705"
     CDS             1893..3086
                     /locus_tag="LNE35_001705"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBK6910849.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="LNE35_001706"
     CDS             3216..4688
                     /locus_tag="LNE35_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6910850.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="LNE35_001707"
     CDS             4771..5559
                     /locus_tag="LNE35_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBK6910851.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="LNE35_001708"
     CDS             complement(5626..6294)
                     /locus_tag="LNE35_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBK6910852.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="LNE35_001709"
     CDS             6409..8121
                     /locus_tag="LNE35_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910853.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="LNE35_001710"
     CDS             8124..9893
                     /locus_tag="LNE35_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910854.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="LNE35_001711"
     CDS             complement(10098..10664)
                     /locus_tag="LNE35_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6910855.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="LNE35_001712"
     CDS             complement(10664..11620)
                     /locus_tag="LNE35_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBK6910856.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="LNE35_001713"
     CDS             complement(11620..12276)
                     /locus_tag="LNE35_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK6910857.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="LNE35_001714"
     CDS             12646..14664
                     /locus_tag="LNE35_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6910858.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    14734..14940
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15091..17505
                     /locus_tag="LNE35_001715"
     CDS             15091..17505
                     /locus_tag="LNE35_001715"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBK6910859.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="LNE35_001716"
     CDS             17853..19406
                     /locus_tag="LNE35_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBK6910860.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="LNE35_001717"
     CDS             complement(19787..21571)
                     /locus_tag="LNE35_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910861.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="LNE35_001718"
     CDS             complement(21586..23307)
                     /locus_tag="LNE35_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6910862.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="LNE35_001719"
     CDS             23407..24168
                     /locus_tag="LNE35_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6910863.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            25135..25403
                     /locus_tag="LNE35_001720"
                     /pseudo
     CDS             25135..25403
                     /locus_tag="LNE35_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            26075..>28306
                     /locus_tag="LNE35_001721"
     CDS             26075..>28306
                     /locus_tag="LNE35_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910864.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         8925 a         5059 c         5989 g         8333 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    24661 aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag aaaaatcaca
    24721 aaggttattg ttatttttat atattctttt taagaatgat tgttttttta tagaaatttt
    24781 aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata ttatagaagt
    24841 tgactggtag tgatttattt ttcgtcgttt tataaaagtg ttattttttg gaaaaattca
    24901 caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta tgactaatat
    24961 aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg gaatcaatga
    25021 aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat agcaatacat
    25081 cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac aacaaaagga
    25141 ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt tattctatac
    25201 aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa tatatggtac
    25261 agaatataga aattattgac attacagaaa atgatgaaga tttaatacag attgtcacta
    25321 tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg attaaggagt
    25381 tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga gcaaaaagag
    25441 taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt ttattagtaa
    25501 tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg tttagttgaa
    25561 ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc caagtattct
    25621 aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt gatgaaggtt
    25681 aaccaataca gggattcact gatgaaattc tagagtctga ccttggtatc aacgatttag
    25741 tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa taactattag
    25801 ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca taattatggt
    25861 taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac agaagacaat
    25921 cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa ccgaaaatac
    25981 gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta tcgggtacct
    26041 tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc ataaactgtt
    26101 aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa cggttctagc
    26161 tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc aagaggaggc
    26221 tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc taaatgcatt
    26281 ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca atgattacga
    26341 acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg caagaagcgg
    26401 tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa gcacaagtga
    26461 gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag aagaaagctt
    26521 aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg aatctcagat
    26581 tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc gccccacttt
    26641 gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg gcgatattct
    26701 tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct taattgagtt
    26761 cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt accaagaagg
    26821 cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag ccgctcagtc
    26881 gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta ctgagccaaa
    26941 taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg aattttcaat
    27001 tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct ctagagaaat
    27061 cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga ccaacagtaa
    27121 taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag tggttaccga
    27181 tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt ccactggaaa
    27241 tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag gtgaagcaaa
    27301 cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta ataaggaaac
    27361 gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt ttattgaata
    27421 tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg atgggttttc
    27481 tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact accgcttgag
    27541 aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca aaaatgttat
    27601 caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt ctggaacaag
    27661 aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt catttctatc
    27721 agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt tcagtgcgaa
    27781 taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg tagaggcgta
    27841 tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca atcgattgaa
    27901 tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta aaactgatga
    27961 gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt cacaagtgat
    28021 acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc ctgtaggtag
    28081 ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga cggtacatga
    28141 tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa acatccttga
    28201 tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc ttcaaggagc
    28261 tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggt
//
LOCUS       DAFCNJ010000033        27564 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000033 DAFCNJ010000000
VERSION     DAFCNJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.034"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            476..1339
                     /locus_tag="LNE35_001722"
     CDS             476..1339
                     /locus_tag="LNE35_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6910865.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="LNE35_001723"
     CDS             complement(1468..3090)
                     /locus_tag="LNE35_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6910866.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="LNE35_001724"
     CDS             3339..3860
                     /locus_tag="LNE35_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK6910867.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LNE35_001725"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LNE35_001725"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBK6910868.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="LNE35_001726"
     CDS             5941..6759
                     /locus_tag="LNE35_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBK6910869.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="LNE35_001727"
     CDS             complement(6862..7059)
                     /locus_tag="LNE35_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910870.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="LNE35_001728"
     CDS             7228..7806
                     /locus_tag="LNE35_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK6910871.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="LNE35_001729"
     CDS             7901..8038
                     /locus_tag="LNE35_001729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910872.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="LNE35_001730"
     CDS             complement(8851..9354)
                     /locus_tag="LNE35_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK6910873.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="LNE35_001731"
     CDS             9713..10738
                     /locus_tag="LNE35_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6910874.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="LNE35_001732"
     CDS             11061..11939
                     /locus_tag="LNE35_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBK6910875.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="LNE35_001733"
     CDS             12415..12531
                     /locus_tag="LNE35_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBK6910876.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="LNE35_001734"
     CDS             13459..13797
                     /locus_tag="LNE35_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910877.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="LNE35_001735"
     CDS             13891..14121
                     /locus_tag="LNE35_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910878.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="LNE35_001736"
     CDS             14521..14892
                     /locus_tag="LNE35_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBK6910879.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="LNE35_001737"
     CDS             complement(15166..15858)
                     /locus_tag="LNE35_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6910880.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="LNE35_001738"
     CDS             complement(15861..16646)
                     /locus_tag="LNE35_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6910881.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="LNE35_001739"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="LNE35_001739"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBK6910882.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="LNE35_001740"
     CDS             18308..18625
                     /locus_tag="LNE35_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6910883.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="LNE35_001741"
     CDS             18638..20311
                     /locus_tag="LNE35_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBK6910884.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="LNE35_001742"
     CDS             20325..21956
                     /locus_tag="LNE35_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBK6910885.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22828..24018
                     /locus_tag="LNE35_001743"
     CDS             22828..24018
                     /locus_tag="LNE35_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HBK6910886.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            24152..26341
                     /locus_tag="LNE35_001744"
     CDS             24152..26341
                     /locus_tag="LNE35_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6910887.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26338..>27564
                     /locus_tag="LNE35_001745"
     CDS             26338..>27564
                     /locus_tag="LNE35_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK6910888.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         8836 a         4564 c         5443 g         8721 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgcgatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactctg ttttcaacgt aaagtgtata aaacagaatg cgatactatc
    22501 aagggagtta aaacaatata cagaacagta aaacactata aataaaaata aaagtgcaat
    22561 aaaagtgcaa tttttgcacc taatcatact ctgtttatat atgaaaacta gattctgaat
    22621 ccaatatttg gttaacgaga ggcgttcgaa tttgaacgtc ttttatatat tactttcaaa
    22681 taaatatgag gtagaatact atctattttc tttttaaccc aattgttata atggagagtg
    22741 taaaatattt tgtgtaaata gaaaaaagga agtcccttct gtagaataga gttaccacaa
    22801 cacattcaca gaagagagga cttccctatg aacgatttta ctacagaaat tctaaagact
    22861 ctagcgaaca aaggcgattt gaatgaatta ttccgtgtcc atttggagaa agcagtcaat
    22921 acgcttctca aaacggagtt aacggctttc ctagattacg aaaagtatga tcgcattggt
    22981 tttaacacgg gtaattctcg taacggctcc tatgaccgta cggtcaagac cgagtatggg
    23041 gaacttcatc tccagattcc gcgcgaccgc aatggtgagt tcaagcaaca gactgttcct
    23101 gcttatagac ggacgaatga cacgttagag gagaccgtca ttcacctctt ccgaaaaggt
    23161 attaccatgt cggaaatcgc agacttgatt gagaaaatgt atgggcatta ctacacgccc
    23221 caaaccatgt ccaatataac aaaatcattt acagaagagg taacggcgtt taatgggcgg
    23281 gagcttcatg accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg
    23341 aaaaccgtcg ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag
    23401 gaagtattga gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta
    23461 ttggaccttc aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag
    23521 gggatggtag gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta
    23581 cacgtttccc gtaatatcag tcataaagtg cgtgtcgatg atcgtaagga agtctgtgat
    23641 gattttaaaa tggtgtatca agcctcatct aaagaggtgg cgttggaagc acgtggtact
    23701 tttgcggaaa aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat
    23761 cacttgctca ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac
    23821 ttgattgaat cctttaataa acaaatcaag aaatacagcc accgcaagga acagttccaa
    23881 aatgaagagt cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc
    23941 ctaggtcgca gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctaagc
    24001 caactgattg agaattagaa acaatctgca gtagggaaga atcatttaca caaaattatt
    24061 gacgctccct tataatgaga acttttacta acataattta tgataaactt actatataat
    24121 catattaatt aaatagttag gagatctgct tatgtttata tctactcttc aaattcgaaa
    24181 ttttagaaat tatgaatccg aaatctttcg attcgataat gagacaaata ctattatagg
    24241 tgagaatgat tcagggaaaa ccaatgctct tactgcattg agaatcttat tagatgatag
    24301 ttattactat agtagtaaaa cactaaaaga atcagatttt tttcatggaa ttcaaaatgg
    24361 gtggcaaggg cattggatta ttatctctgc gacatttgaa ggaatcagcg aagaagaatt
    24421 tgacaatgag atttgtgctt ccatttcttt aaattctgaa agtcaaacaa tcttagaaga
    24481 attgatttca aatgcagata aaggtgtagg tagtatttct ctatttatta gacctaacaa
    24541 agctattaga aagcaattat ttgatatttc cactgaatta gactctcatc aatttaacga
    24601 atttagaaat tctattcgtc tatccgacta tgaattttac tacacatcta aatcaaatct
    24661 aaatttctgt atcaatgaaa attatgataa attggtagga agactcaatg acaatatagc
    24721 tagtaatcct gaagaagacg atgaagctct gttgggctca agaattgaca tgtcagatat
    24781 tttcaagcat atttcggttg tttatgttga cgctcttcga gatgttctta gagaaatgaa
    24841 aaacaatcga aacccagtca aaagaatcat ggaaacaatt gaatcaaaaa tatcatcaga
    24901 taatgttgag tcattaaaaa caattataca gcaattaaat gagactatta ccagtgttcc
    24961 tgaaattagg aaaattggcg aaaatattaa tcggcaacta aatagcataa ttggttcagt
    25021 gtattctcct aatcttcttc tttcctcaac tatgtcagat gatatgggtt ctttagcaaa
    25081 atttctttca atgaagccag aacaaaatat cgacttagac ttactagggt taggacatct
    25141 aaatatgatt tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact
    25201 acttaatatt atgttgattg aagaaccaga agctcatatt catcatcata ttcaaaaaac
    25261 tctgttcgaa ggattaaatc ttcaaaaaaa ctatactcag attctcatga ccacacattc
    25321 tgtacattta gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg
    25381 caaatctatc gttatgaatc cagcaaataa tctgaactca tttgctaaag aaaagttaaa
    25441 aaagaacaat ctaaatctaa taaacgcagt agagagatat ttagatgtta aaagaaatgg
    25501 attgcttttt tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa tacttattcc
    25561 tcaaatagta aaaaaattat ttggtataaa cttagacgaa cttggaatag gtttgattaa
    25621 tgttggtagt acttcgtttg aatatatcgc tagcttgttt gacgatgata gaatacagag
    25681 aaagtgtgct attgtaactg atttagatga acaggcaatc ccatcagaac atcgattgta
    25741 taaaagcact gcacaagaga aaggtcaagc aagaaaagaa aaattatcct ccctttttaa
    25801 tgaaaacaat tgggttaatt gtttttatgc taatacaaca tttgaaattg agtttttaga
    25861 tgtgaataaa gaaaatattc taacttatgt cagtccaatt atagatgacg cattcaaaga
    25921 taaaaaaaca ataactgctt ataaagaaga aatacaaagt gaacaatatt tagaaagaaa
    25981 tttagcgatg ttaaaactag ccaatcatgt tggtaagggg tggtttgcaa ttgagttagg
    26041 tgaaaaaatt gactttttaa tcaaaattcc taagtatatt cttaatgcta ttgcttttgc
    26101 atgtcaggaa atagtagatc tctcaattta taaaaaaatt ctactccata caatcgactt
    26161 gtacgaggaa actacagaat tgtcagcttt atcagctagt ttaaaaacaa gtgatacagc
    26221 tgatttgaat atagcaataa tttctttatt agagtacttt ggtaaagatg aggatgttgt
    26281 gtatctaatc aatcagatag aaaattacac aaaaatactt tatgctgagg tttcaaaatg
    26341 acaaatatct tcgaaaaaat cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa
    26401 agtgaagata acctttatgt aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca
    26461 aggaaaattg cttaccaagc aattaaacac gatggatcac taaaaagaat aattgctatc
    26521 acatacacta atagagctgc tgaagaaatt aaggaacgcc taagtttact tagtatagat
    26581 gatgatatta atatttgggt ggggacaata catcagtttt gtcttgaatt tattatttat
    26641 ccttttaaaa tgaatctctc aagagtatca aaaggattta caattattga tgattatact
    26701 caaagagctt acgtcagcca aattaataat atgctgaatt taaatctcaa aacgtatgaa
    26761 ctgaacaaaa ttgatttgtc actttctcat gattttaaaa ttaatgaagt tcagtatact
    26821 caagtttctg aaatgtatca cactatgttg gaaaaaaata aagaaattga ttttgatatg
    26881 attttactag tagcgtataa aattttaact gtaaacagta acgttgcacg taatattgct
    26941 agtaatataa gggcgattta tgtggatgaa tttcaagata ctagagaatt acaatacaat
    27001 gtaatagcta aattacttca aaacaaccct caaattcagt caatgtttgt tggagatatt
    27061 gaccaagcaa tttatggatc attagatggt atagctaaat cagttgaaga actagaaatg
    27121 ctaactagtc acacttttca atcaaagacg ctacatggct gttacaggtc taatcagaga
    27181 ttagtagact tctactctaa ttttcagagt tgtccttacg agattgaaag cagagggaat
    27241 aataaaaacg accgtggttt tataagttat gattgcaaag ttactaagga ggatttaccc
    27301 aacataataa aaaatataat aaaggaaaag attgaaagcg gcattcatga aaaagatatt
    27361 tgtgtacttg ctcctcaaca ctatcctctg ttttcactgg gtgagtattt aaagaaagaa
    27421 ttaccgtatt gcaattttga tgctataaat atatcaccaa ttaaggtaaa taatcatagt
    27481 ttatttttca agttagcaat tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga
    27541 aagctaattg caaacgatat ttta
//
LOCUS       DAFCNJ010000034        26605 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000034 DAFCNJ010000000
VERSION     DAFCNJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.035"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1152
                     /locus_tag="LNE35_001746"
     CDS             <1..1152
                     /locus_tag="LNE35_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBK6910889.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="LNE35_001747"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="LNE35_001747"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBK6910890.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="LNE35_001748"
     CDS             2728..3003
                     /locus_tag="LNE35_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBK6910891.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="LNE35_001749"
     CDS             3009..3776
                     /locus_tag="LNE35_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBK6910892.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="LNE35_001750"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="LNE35_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBK6910893.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="LNE35_001751"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="LNE35_001751"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBK6910894.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="LNE35_001752"
     CDS             7920..8222
                     /locus_tag="LNE35_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBK6910895.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="LNE35_001753"
     CDS             8225..9388
                     /locus_tag="LNE35_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK6910896.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="LNE35_001754"
     CDS             9414..12842
                     /locus_tag="LNE35_001754"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBK6910897.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="LNE35_001755"
     CDS             12863..13987
                     /locus_tag="LNE35_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910898.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="LNE35_001756"
     CDS             13977..14414
                     /locus_tag="LNE35_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK6910899.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="LNE35_001757"
     CDS             14398..14736
                     /locus_tag="LNE35_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBK6910900.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="LNE35_001758"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="LNE35_001758"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBK6910901.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="LNE35_001759"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="LNE35_001759"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBK6910902.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="LNE35_001760"
     CDS             15893..16936
                     /locus_tag="LNE35_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBK6910903.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="LNE35_001761"
     CDS             17095..17823
                     /locus_tag="LNE35_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBK6910904.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="LNE35_001762"
     CDS             17939..18184
                     /locus_tag="LNE35_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK6910905.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="LNE35_001763"
     CDS             18181..18399
                     /locus_tag="LNE35_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK6910906.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="LNE35_001764"
     CDS             18535..19074
                     /locus_tag="LNE35_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBK6910907.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="LNE35_001765"
     CDS             19067..21340
                     /locus_tag="LNE35_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBK6910908.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="LNE35_001766"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="LNE35_001766"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBK6910909.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="LNE35_001767"
     CDS             complement(22513..22767)
                     /locus_tag="LNE35_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBK6910910.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="LNE35_001768"
     CDS             complement(23167..23436)
                     /locus_tag="LNE35_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910911.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="LNE35_001769"
     CDS             complement(23906..24361)
                     /locus_tag="LNE35_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBK6910912.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="LNE35_001770"
     CDS             complement(24499..26427)
                     /locus_tag="LNE35_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK6910913.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8861 a         4610 c         5614 g         7520 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taaga
//
LOCUS       DAFCNJ010000035        26132 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000035 DAFCNJ010000000
VERSION     DAFCNJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.036"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..310
                     /locus_tag="LNE35_001771"
     CDS             <1..310
                     /locus_tag="LNE35_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6910914.1"
                     /translation="QIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNL
                     IEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF
                     "
     gene            783..1337
                     /locus_tag="LNE35_001772"
     CDS             783..1337
                     /locus_tag="LNE35_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6910915.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1510..1983)
                     /locus_tag="LNE35_001773"
     CDS             complement(1510..1983)
                     /locus_tag="LNE35_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6910916.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1995..2531
                     /locus_tag="LNE35_001774"
     CDS             1995..2531
                     /locus_tag="LNE35_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6910917.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2571..2942
                     /locus_tag="LNE35_001775"
     CDS             2571..2942
                     /locus_tag="LNE35_001775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6910918.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            3226..4497
                     /locus_tag="LNE35_001776"
     CDS             3226..4497
                     /locus_tag="LNE35_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK6910919.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4742..5767
                     /locus_tag="LNE35_001777"
     CDS             4742..5767
                     /locus_tag="LNE35_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6910920.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            6061..7170
                     /locus_tag="LNE35_001778"
     CDS             6061..7170
                     /locus_tag="LNE35_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK6910921.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            7253..8149
                     /locus_tag="LNE35_001779"
                     /pseudo
     CDS             7253..8149
                     /locus_tag="LNE35_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            8165..8989
                     /locus_tag="LNE35_001780"
     CDS             8165..8989
                     /locus_tag="LNE35_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6910922.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9021..10331
                     /locus_tag="LNE35_001781"
     CDS             9021..10331
                     /locus_tag="LNE35_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HBK6910923.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10479..11738
                     /locus_tag="LNE35_001782"
     CDS             10479..11738
                     /locus_tag="LNE35_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6910924.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11748..12620
                     /locus_tag="LNE35_001783"
     CDS             11748..12620
                     /locus_tag="LNE35_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6910925.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12632..13462
                     /locus_tag="LNE35_001784"
     CDS             12632..13462
                     /locus_tag="LNE35_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6910926.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13502..15685
                     /locus_tag="LNE35_001785"
     CDS             13502..15685
                     /locus_tag="LNE35_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK6910927.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15746..16966
                     /locus_tag="LNE35_001786"
     CDS             15746..16966
                     /locus_tag="LNE35_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HBK6910928.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16963..18423
                     /gene="gtfA"
                     /locus_tag="LNE35_001787"
     CDS             16963..18423
                     /gene="gtfA"
                     /locus_tag="LNE35_001787"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBK6910929.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18500..19606
                     /locus_tag="LNE35_001788"
                     /pseudo
     CDS             18500..19606
                     /locus_tag="LNE35_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19928..20137
                     /locus_tag="LNE35_001789"
     CDS             19928..20137
                     /locus_tag="LNE35_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBK6910930.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            20130..20279
                     /locus_tag="LNE35_001790"
     CDS             20130..20279
                     /locus_tag="LNE35_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBK6910931.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20433..21098
                     /locus_tag="LNE35_001791"
     CDS             20433..21098
                     /locus_tag="LNE35_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6910932.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21382..22467)
                     /gene="xerS"
                     /locus_tag="LNE35_001792"
     CDS             complement(21382..22467)
                     /gene="xerS"
                     /locus_tag="LNE35_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBK6910933.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22801..23034
                     /locus_tag="LNE35_001793"
     CDS             22801..23034
                     /locus_tag="LNE35_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HBK6910934.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            23031..23438
                     /locus_tag="LNE35_001794"
     CDS             23031..23438
                     /locus_tag="LNE35_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBK6910935.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24575..25093)
                     /locus_tag="LNE35_001795"
     CDS             complement(24575..25093)
                     /locus_tag="LNE35_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBK6910936.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         8921 a         3983 c         4843 g         8385 t
ORIGIN      
        1 tcaaataagg tttatgatag ataaatggaa aaagcagtat ccacgagtgg ttaaattgct
       61 catgaatcct gctatattaa ccttttataa tttccctccg tcaatcagaa gaactattta
      121 ttcaactaat ttgattgaag gttttaacaa acaattaaag aaatatacca aaagaaaaga
      181 gcaatttcct aatgaagaat ctctggagag atttctagta tctcaattca acaactataa
      241 tcaaaagttt ctatgccgag ttcataaagg ttttaaagaa atacatgata cattagaatc
      301 aatgttttaa cttgatcaac agaatgggct tttccattta cacaaaatta ttgacgctcc
      361 cgacaaaatt atgatcttgc gtaaagttat aaaatattaa taaaaatatt ttattaatga
      421 aaagcttggt agcacggtta ttcatatgct aatttatttc actttatagt aaggggagtt
      481 cttagtatat tgttcaaact taaatttcat ataatttaat tatgtaaatt agctattcta
      541 agaacttcta cggataactg cccgtctaaa atttaaataa gagcttagaa tcttcatata
      601 agtaaggtta atttttttgt acaaaaaagt cggaatattc tgagttactg aaatgatata
      661 aaatcataaa ctaaatccct tgattttatt gactttcaaa agtttatata tataagttaa
      721 tgattatggt aaaatatata cataatcatt aactcagaat atttttaaga agggaaacaa
      781 tagtgagcta taatgtacaa ccgttaagga caaatgaaga gatacaagat tttcagtttt
      841 ggctaagaag aacaagtaat ccggaaagag atagtttttt atttttgttt ggaatcaata
      901 atgggttacg tatgtcagat attataggat taaaagttgg agatatacga ggcaaaagta
      961 agcctattat tgttgaacat aaaacaggta agcgaaaacc gatttttata gataatttga
     1021 gagaagaaat tttattatat actgaaggta aagaagaaaa tgattggtta tttccgagtc
     1081 ggcaacaagg tcgacatata acaagggatc gtgtctatca gatatatgca gatatagcag
     1141 aaaaattagg acgagatgat ataggaacac atacattaag aaaaactttt gggtatcact
     1201 actacaagaa aactagagac attgctacct taatgtttat ttttaatcat agctcgcaag
     1261 caattacaaa aaggtacatt ggtataactg aagatgaaat aggagcatca cttcgtggat
     1321 ttaaactagg tgtataattt aaaaattttt ataaacagta aagacaaaga ttaaaagcta
     1381 tttttatcta gaacgatttt atttcataaa gttcgcggaa taaaagctgg ggttactggt
     1441 acttggtata ggttcgtgtt atataaacta agtaattaaa taacattttt aggttggaaa
     1501 aggatgaaat taaaaaaaga gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa
     1561 gaaaaatact gataactcaa cgtttagcag actatctaat agggatagtc tgcttttttc
     1621 gtttttgacc atacttttga ccttctgcaa acttccttgc ttttcctctg tagaactcat
     1681 gaaatctgca aaacgaattg ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat
     1741 atcatccata gtggttttaa tatctgtatg tccaagtcga acatacactt ctttgacaga
     1801 cgcaccagat tcaaagagca gactacaatg ggtatgacga aaaccatgaa gtgtgatatg
     1861 ttttaagtta ttctctttaa tgatgatttt caaattgtga tttacatagt ctaaataaag
     1921 gggcttattt tcttctgatg aaaacaaaag ttgttcacca tcttttatag gtttgattct
     1981 catttttaga tattatgccc gcacgtattc atcacatttc tattgtgaat cgctttattc
     2041 gaccgacatt tgacttttat cataatatac tcggtttaaa attactaatg aaaacaatta
     2101 atcaagacga tcataccatg tatcacctgt ttttttcaga taatcaccac cgtgtgggaa
     2161 ctgaactgac tttttttgaa gtacaagaag ggaacaacca gttttttgga accaatacga
     2221 ttgaacgaac gatactaaaa gttccttccg aagcatcatt gcatttttgg gaaatatatt
     2281 ttgaaacaca aggcgtttgc cattatggaa tcgaatcatt ttctggacga cctatcttac
     2341 gttttgaagg gccagatgca acacgattag ctttagttcc cttacgcgaa tttgaagata
     2401 ttgatgatta tttcccgtat gtcacagacc agatccctac agagcatgcc attttaggaa
     2461 ttgatgccat tcaactacgc gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg
     2521 taggctggta gcacaaagaa tacgtcagtt tcttttcggg tcatcaacga gtgacaatct
     2581 tagaaaatca agacactcag ttctatcaag aagttcagat tattcaagat cgtgagaatc
     2641 cagttgccat tgaagggatt ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat
     2701 ctgatttaca acgcatcgac aaacaattac aagagcgcaa ttttataaat tcaggaataa
     2761 aagatcgtga attttttgta tcactttatt atcgtgaccc aaatcaatta ttgattgaaa
     2821 ttgctacagg ggaaggaaac ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg
     2881 aagaaattcc tttattttta ccgcagtact taaatttcat tcgtgaacaa gtggagcaat
     2941 aattagctaa tcaaatccat gacgaagagt aagaggttga ctcataagtc aaaaaataaa
     3001 gaaaaacgca tcaaatccat tctaaagaat gttgatttga tgcggttcct atttttataa
     3061 actatcattt tcgactaata aaactactta tgagtcagcc tctctttttt tcttgatttt
     3121 tatacctctt tctaggtaaa ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt
     3181 ctcaactatt tcaaaataaa ggggagtacc aaatgaacga gtatattgaa attatccacg
     3241 caagagaaac aacttaaaaa tgtcagtctc aaaattccca aaaataaaat tagtattttt
     3301 accagcgtat ctggttcagg aaaatcatcg attgtttttg atactattac ccaagaagct
     3361 ggtcgtcaac tgaatgacac ctatgatagt tttacccgtt tatttttacc taaatacagt
     3421 cgtccagagg ttgatgagat tgataatctt tctacggcga ttgtggtcaa tcaaaagaag
     3481 attggtggca gtgcgcgctc aacgttaggg acgattacta acatcaatgc gttatttcgt
     3541 gtgttgtttt catgttttgc aacacctagt ttaggatatg caaatgctta ttcatttaat
     3601 gatcctagtg gaatgtgttc tgtctgtcag ggaatcggta agacgattgc atttttaata
     3661 ggattgcttt atttttcggc tattgataat gctgattttg tattttctga tccatttacc
     3721 gcagaagttt tgtctggaat tgaggaacag gcaaagaatc atggttattt tgtattggtg
     3781 cataatgtta attcagcaga agatgttaaa actattcaga gaaattggcg ttttgagggg
     3841 ttcattgttg ttggtgtacg tgtaaaagaa tttttggaat taaataacgt ttttgatggt
     3901 cctgttgttt atattgatac gcatctttca aaagagacat taagagaact tgaacaacaa
     3961 atgaatcgtt tatttgtaaa tacaaatgat taccaagcta gtacattggc agtggaacat
     4021 ttgattcatt atggtcatga gaaaatagcg tttctttctt ttgactacat tgtaggtgaa
     4081 ccaagtgtta ttcaagaacg acatcaagct tatatcgata cattaaagaa atatcattta
     4141 acctttaaat cgcattggat atataacaac tctgaatttg agcgaattta tcaagaatta
     4201 gctgaattta ctgcaattgt tgttactgga gattatttag cggctaaatt agtaaaattt
     4261 ttaaaaaata agaataaacg agtgatggaa caattatcga ttattggatt tgatgatatt
     4321 acttttgcag aattaatgga tcctgcgttg acaacaatac gactaaatcc aggtaacaag
     4381 ggggcgattg cttttcacca actgattgga ctggccaaac aagaagaaat aaaaaatcaa
     4441 gtactactat tagagggaga attgatttct cgagactctg ttcacaaaat caaataaaaa
     4501 agctctgtga aataagtata gttaacaata aaatatccga acgatagaga gaatgaactg
     4561 tgatctagca gttcctcgtc gcaaattcac tagaattagc attatttact ctaaattgtt
     4621 cggatatttt ttcttgtttt taattatgat tacagtgcct atttttaacg aaatttgctt
     4681 attttcaaaa tgtatgatta tatgttagta aacactttca aatatgaaaa gaggagctat
     4741 tatggtagga attagggatg ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg
     4801 cgtgctaaat gaggatccga cactttcagt aaccgatgaa accaaacgaa agattgcaga
     4861 atcggttaat ttttatcatt atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat
     4921 tgcattaata acgacagttt ctgaagtaga tgagttggaa gatccctatt ttcgaacgat
     4981 tcgcagaggg attcaagtcg aagcagaaca aagcaaagtc aatttgaaga aaattattcg
     5041 tttgtcagaa acaccgttgg agcaagacga tttgctaaaa tgtcaaggtg tgttgattat
     5101 tgggcaggtg ctatcttctg tgattgaaga agtatcagca attaattcga atgtcgtagt
     5161 gattgatgac cctagtattg aagctgaagt agatgcagtt tatactgatt taacaaaagc
     5221 aacgaatcag catttagatc gattgtataa aaaaggacac cgaaacattg cgtttatcgg
     5281 aggaggacgt gttcgtttag atagccaagg aaataaacac gtgtcagatg atgattatcg
     5341 aaaaatagcc tatgagacgt ggatgaaaca aaaaggatta gaaaaatata gtcaaattta
     5401 tttagaaggc tggaccacac ttgacggaat gcaggcagca gatttacttt tgaaaaaaca
     5461 tgctaaagat aagttgccga ctgctatcat ggtaggaaat gatcctattg ctgtgggggt
     5521 ttatcgttca cttcaaaaaa atggtctaca tattccagaa gatatttcga ttgtgagttt
     5581 tgataatatt gaagttgctg aatttttaac acctgcatta aatacggtga atgtgaatac
     5641 agaagaaatt ggaaaactag cagttcgcat ggttaatgaa cgaataaaag aagttagaaa
     5701 tgtttcgatc aaagtgattg tttctaataa tattattgtt agagaaagtg aaaaaaatat
     5761 cgattaaaaa agtaaacatc caaaaaaggg tgtttacttt tttgtttact aatattttta
     5821 gtaaaaaatc tcgacaaaac attaagtaaa cagttatggt gttgttaaca aaaagaaaac
     5881 gcattcaaaa caggaggaat taagatgaaa catttcaaaa aaatagcttt aacagggata
     5941 gctgttttaa ctgggacatt actcgcagca tgtggtgggg gagatagtaa ttctaaagac
     6001 gggcaagtaa ctttaagcta tgcgagttga gatactgacc aagctccagg actaagaaaa
     6061 atgttagatg aatttgaaaa acaaaatcct gatatcaaag tagaaatgga gacaacgcct
     6121 tgggatcaat attgggttaa attagaagct gcaacaacgg gaggtaatat gccagatgta
     6181 gtaaccatgc actctagcga atcctataaa tatatgtcac agaatgcttt gatgggactt
     6241 gatgatgtta tcaaagaaaa tgatattgac atgggaaact ttactgaagg aattgctgat
     6301 ttctatacgc ttgaagacga actatacgca atccctaaag atgcttctgt agtggggcta
     6361 tggtacaaca aagaattatt tgataatgca ggtgtcgatt atccagatga aacatggaca
     6421 tgggaaacac tcagacaagc tgcaattgat ttaacagatg aatcaaaagg catctatggt
     6481 tttgcatcac atattggaac agagactggc tactggccat ttatttatca aaatggggga
     6541 gaagtatttg ccgatgacaa tacaaaatca ggtttgaata ccccagaagc acaagaagct
     6601 ttagcttttt atcgagatat tgcacttgtt gatcatgctt caccaacaat tacagaatta
     6661 caagatcctg ataagttaag tcgttttcaa tcaggtagat tagcaatgat gattgaagga
     6721 aactggtttg caccgggatt tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc
     6781 gtgttaccta agggaaaagt taatgcaaca attacaaatg gacttggttg ggctgccagt
     6841 gcgagtacaa aatacccaga agaagtgaaa aaactaatgg catttttagc tagtgaggac
     6901 gccaataaaa tccaagcgga aacgggggca tcaatcccag cactaaaagg ctttgaagac
     6961 gagtgggctg cttcttcaga tacaattgat ttgagtgctt ttggaaaaat gttggagtat
     7021 ggagtccctc gtccatacaa taaagatgga ttacgagctg aacaagcaga agttcaaatt
     7081 attaaccgta ttctgtctgg tgaagtaact gttgaagatg gaaccgctca aattgaaaaa
     7141 gaagtcaatg cgatattgag cggaaattaa attagttaat tatacaatat acaggcgaaa
     7201 ggattctatt tcgtctgtat attaatggat gaataccaaa aagggggata ttatgaaaaa
     7261 tcgaattttt ggaaaatatc gtaaagatca agtgatatgg ggctatattt ttatttttcc
     7321 aacggcatta ggattaattt tattaaatat atggccagca attcaaacag caattttaag
     7381 ttttcaaaaa acggtagggt tcggtgatac tgaatgggtc ggattaagaa attaccaaaa
     7441 aatgtttgca gacggtgaag tttttcagtc tttattaaac acattaattt atgcagtggt
     7501 ttcgatacct agtattgttg tattatcctt gttggtggca gttttaatga atagaaaagt
     7561 taaaggattg tcgatttatc ggacaattta ttttctacca gtcgttgctg caccatccgc
     7621 agtcgcaatg atttggcgct ggatgttcaa taatgattat ggactgatta ataacatgtt
     7681 ggctaaaata ggtctcgatg gaccagcatg gttaacggat ccatcgattg cgatttattc
     7741 tattatcatt gtaggagttt ggagtgctat tggttataac atggtgttat tactagccgg
     7801 cttataagaa attcctaaag attattatga agcagcggaa attgatggtg ctagtccaat
     7861 ccagcagttt ttcaacatta cgttaccatt agttacacca acattatttt ttgtagttgt
     7921 aacaacagtt atcaatgctt tccaagtatt tgatgttatt tttatgatga ttactcctaa
     7981 tagtaccgcc atgtttaaat cacaatcatt agcctattta ttctataaac attcatttat
     8041 tttaaatgac aagggatatg gttcggcaat cgttatgttc cttttagtga ttatattaat
     8101 tattactgca attcaaatga agttgcagaa aaagtgggta aactattaag gaggctatca
     8161 aattatgaaa aataaatttt tttcaaatgc gccaatccat atcatgctta ttttaggtgg
     8221 tattgcaatg gcattacctt ttatttggat gattttaact tcagggaaaa cgttggttga
     8281 atctacccaa attccaccta caattttccc tgagaaattt cagctaagta actatcaaca
     8341 agtacttgat ttattaccat tcatggcttt ttatatcaat acatttttaa tggttgttgg
     8401 acgagtgatt ggctcggttt tattttcagc aatggctgca tatgcttgtg cgcgtctaag
     8461 ttttccaggg aaaaatatcc ttttcagctt agtacttatt caaatgatga ttcccggttc
     8521 catttttatt actccacagt atttattagc acaaaaattg ggtattttaa atacaattcc
     8581 agcattaata ttaccagggg tagttagtgc gtttggtacg tttttattaa gacaattttt
     8641 cttaggttta cctgatgaat tggaggaagc ggcctattta gaaggtgcca atatttggac
     8701 gatctttttc aaaattatgt tgccgcttgc taaatcagga ttggttgcac ttggaatatt
     8761 cacagcgttg tttgcattta aagatttaat gtggccattg attgtcaata cttcaattga
     8821 taagatgcct ttagctccgg gattagcaaa cttacaagga caatatgcca cgaacttccc
     8881 acaattaatg gcaggttcgt tattagcaat ttggccaata gttgtgatat tcatcgtttt
     8941 ccaaagaaaa ttcattgaag gaattgctac aacaggtagt aaattataaa actattaatt
     9001 agtgattgga gagtaagtac atgacaaaaa ttacatttat tggtgcagga agtactattt
     9061 ttgcaaaaaa cgtattaggc gatgcgatgt taactccaag tctacaagat gcacatattg
     9121 cattgtacga tattgatgaa aaacgactaa aagaatcaaa tttaatgctt aaaaatatta
     9181 ataaaaattc gaatgaaaat cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt
     9241 taagagaggc tgattttgtt atcaatgcta ttcaagtggg tggttataaa ccttcaacag
     9301 tgattgattt tgatattcct aaaaaatatg ggttgcgaca aactattggt gacaccatgg
     9361 ggattggtgg gatttttaga accctacgga cactaccagt aatgttagac tttgctaaag
     9421 aaatggaaga agtttgtcca aatgcttggt tattgaacta tacaaatccg atggcgatgt
     9481 taaccatggg tgttttaaaa gcgacgagta ttaagactgt tggtttatgt catagcgttc
     9541 aagtatgcgt accagaattg tttgagcatt taggaatagc cgatcaatat gacttaaatg
     9601 actttcaatg gaaaattgca ggcattaatc atatggcatt cttgttggaa attacaaaaa
     9661 atggggaaga tttctatcca acaattcgtc agttagctgc agaaaaacca aatccacatc
     9721 cagattctgt acgttttgaa ttgttgaaac gttttggtta ctatattaca gaatcaagtg
     9781 aacacaatgc agaataccat ccatacttta ttaaatcaaa atacccagaa ttgattgacg
     9841 aattagggat tccgttggat gaatacttac gtcgttgtga acaacaaatt aatgattggg
     9901 aaagcatgcg agaggacatt gtaaataatg gggagttaac acatacgaga agccgtgaat
     9961 atgcttcata tatcatggat tctattacga caggaactcc gtcagtgatt gcaggaaatg
    10021 tcttaaataa aggcttaatc acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt
    10081 tagtcgataa aaatggcgtg caaccaacct atgtcggtga attgccaaca caattagctg
    10141 ctttaaatcg gacgaatatc aatgttcaag aactgacagt agaagccgct ttaacactag
    10201 ataaatcaaa aatttatcaa gcagcgtatt tagatccaca tctatcatct gaattgtctt
    10261 taacagatat caaaaattta gtggatgatt taatcgaagc acatggggac tatttacctg
    10321 aatataatta aaaagaagct gaaagagtaa ggaattttca attccttact cttttcttag
    10381 ttaaatttag taaaaatatt gacaaaacac ttagtaaaca gttacattag tgttaacaaa
    10441 aagaaaacgc attcaaattg tataaggagg agcataaaat gaagtggtct aaaaaaatta
    10501 ttttagcttg tatagcacta ggagcaacga tctctttgac tgcttgtgga aaaaatcagg
    10561 aagatgcaag cggtaaggta gaaatagagt tttttaatca aaaaaaagaa atgactcaaa
    10621 cgattcagga gattgctaaa gattttgaag caaaaaaccc tgatattcat gtaaaagttg
    10681 tggatgtacc gaatgctggt gaagtaatta aaacacgtat gcttgcaggc gatgtaccag
    10741 atgtcattaa tctttatcct caaagtatag aattgaaaga atgggcgaaa gctggttatt
    10801 tagaggactt aaccgaagaa ccgtatttag agaatatcaa aaatggatat gcacaacgtt
    10861 ttgctattga ggatcgtgta tatagcatac cattaaccgc taatgtctat ggtttttact
    10921 ataacaaaac tgcttttgag gaaatgggga ttcaagcacc agaaacttgg gcagaatttg
    10981 aaaaaatagt agctgagatt aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag
    11041 aaggctggac tttaaatggt tatcaccaac tagctatagc gactgctgct ggtggtgaag
    11101 aggaagcgaa caatgtttgg cgtttctcag aagttaatgg aattaatgct gaaagcaaag
    11161 aaatgcaaaa agattttaat cgattagatt tgttaagaga accaaaagct ttgcaaaaca
    11221 actggcaagg tgccggttat aacgatacag tagttacttt tacaaaaggt gatgcgttga
    11281 ttatgccgaa tggttcatgg gcaatgccga tgattcattc acaaaatcca gattttgaag
    11341 taggaacttt tccttttcca gcagatgaag caggtcaaag tctaacgatt ggcgcaggag
    11401 acctagcctt atcagtttct gcaacttctc aacataaaga agaagccaaa cgttttgtag
    11461 aatatatgac aacacctgaa gcgatgcaga aatattatga tgtagatggt gcaccgtgtg
    11521 ctgttgaggg cgttatcgag aatactcagg attcaccatt aagtggttta actgaacttg
    11581 cttttactga tcgtcattta gtttggttag ccaaagattg gaatagtgaa aatgattttt
    11641 ataccttaac aacaaattat ttacacaatg gcaatcaaaa agccatgatt aacgcattaa
    11701 atgctttctt taatccaatg aaagctgacg gaaggtagga aaataaaatg aaaataaaag
    11761 aaattttaaa tcgttactgg gcaatcattt ttgtagcaat tccaattatt ttgcagatga
    11821 tttttttcta ctttccaatg gttcaaggtg ctttttacag tttaactaat tggactggtc
    11881 tgacttataa ttttgatttt gtgggtttga acaattataa gattcttttt tctgatccga
    11941 agtttatcaa atcgattggc ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa
    12001 ttggttttgg tattttgatt gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct
    12061 ttagagcatg gttctttttt ccagcggtct tatcgggcgt ggttgtttct ttagtattta
    12121 aacaaatttt taactatggt ttaccaaaaa ttggtagtat gttaggcatt gaatttttac
    12181 aaaccagtct tttaggaaca acatggggag cgatttttgc agtaattttt gttttacttt
    12241 ggcaaggaat tgccatgcca gtcatcattt tcttagcggg ttttcaaagt attccacaag
    12301 aaattattga agctgctaga atggatgggg cgaatgataa gcaaattttt tggaatattg
    12361 aaatcaccta cttattacca agtatttcaa tggtatttat tatggcatta aaaggtggct
    12421 taacagcatt tgatcaaatt tttgctttaa ctggtggtgg accaagcgat gccacaacat
    12481 ctttggggtt acttgtttat aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg
    12541 ctattgcttt agtcttattt atcttaattg ggattgtttc actggcacaa ttaaaattat
    12601 cgaaaaaatt tgaaatctag gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc
    12661 taaatacttg cttttattat tagggggctt catgatttta gttcccttag tttcaacgct
    12721 attcggttca tttaaaacaa cacccgatat tatgaataat ttctttcgtt ttcctaatcc
    12781 agcgactttg gaaaatttcc gaagattgtt agcggatgga attggtcatt acttttggaa
    12841 ttcggcaatc atcacaatca ttgcagtagt tgctgtgaca gcaattattc ctatggctgc
    12901 ttattcacta gcaagaaaaa tgactcgtgg gaaagcatac ggattgatgt atttattttt
    12961 aattttagga atttttgttc ctttccaagt aattatgatt ccgattaccg ttatgatgag
    13021 taaaattggt ttagcaaata tgtggggatt aatcttactc tatctcactt atgccgtacc
    13081 acagacacta tttttatatg ttggttttat taaactaaat gtgcctatca gtctggatga
    13141 ggctgctgaa attgatgggg ctagtcactt tacaacttat ttcaaaattg ttttcccaat
    13201 gttaaaacca atgcatgcga cgactattat tcttaatgct ctatggtttt ggaatgattt
    13261 catgctacca ttattaattt taaatcgtga ttctaatatg tggacactac cattgtttca
    13321 atataattat acgggacaat attttaacga ctatggtcca agttttgctt cctatacagt
    13381 aggaattgtc acgattacac tagtttatct tgctttccaa agaagtatta tagctggaat
    13441 gagcaatgga gcagtgaaat aaccaataaa ttattctcat tataaaggag ttcataaact
    13501 tatgattata tttgacaatc acacacaaac atttcattta caaaataaaa aaattagcta
    13561 tctattagga atcgaagaaa ataattattt aacgcatctt tattttggaa aaaagattac
    13621 gcactattct ggtgggtatc gctacccccg aacagatcgt tccttctcac ccaacaatgc
    13681 aaatgcgcaa gatcgtttat tatctttaga cacattacca ttagaatttc caggatatgg
    13741 acatggtgat tttagagaac cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa
    13801 tgattttcgt tatgatagct acgaaattca aaaaggaaag cccagcttag cgggattgcc
    13861 agccttatat acggaagatg atgatgaagc tgaaacgctg attattcaat tgaaagacgc
    13921 gattagtgga ttgactttac agttgttcta cacaatttat caaaatcaca gtgtaattat
    13981 tcgttctacg caattagtaa ataatagtga tgaaacagtg gagattaacc gcttagctag
    14041 tcaatcaatt gattttccaa atcgtccact cgagttaatt catttaaatg gtacctgggc
    14101 aagagaacgt caaatgacta gagaaaaaat tcatacaggg ataaaagtat tagatagtaa
    14161 acgcggatca agcagccatc aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga
    14221 tgaatttcaa ggagaggcat atggatttaa tttagtttat agcggtaatc acgaaacggt
    14281 gattcaaaaa gatccatttg accaaacacg ggtcattatg ggaattaatt catttaattt
    14341 tggttggcaa ctaaaaactg gcgactcatt tcaaacgcca gaagtcgtga tggtttatag
    14401 taatgaaggt ctgaatacgt tatcacagac gtatcatgaa ttgtataatc accgtttaat
    14461 tcgtggaaac tatcgtttaa aagaacgtcc cacgttaatc aacaactggg aagcaacgta
    14521 ttttgatttt aacgaagaaa aaatcatgaa tattgtcgat gatgcgaaga acttgggcat
    14581 tgaaatgttt gttttagatg atggttggtt tggcggtcgt gaaaatgact tcacttcatt
    14641 aggtgattgg gttgaaacag aaggcaaact tgcttgtggt ttggaaaatt tagccaagca
    14701 agtgcatgat aacgggatga aatttgggat ttggtttgaa ccggaaatga tttctgaaga
    14761 tagtgaacta tttcgagcac atccagattg ggcattgggt attccaggtc gtgaccgctc
    14821 aattagtcga aatcaatatg ttcttgattt ttcaagacaa gatgttcgtg ataatatcta
    14881 tcaacaaatg acggcgattt ttgatcgtgt accgattgat tatgtgaagt gggatatgaa
    14941 tcgaaatatg acagaagtct attctgtctt gttggactca acgatgcaag gtgaagtggc
    15001 tcatcgttat atgttagggt tatatgaatt tttagaaaag ttaacaacga attatccaac
    15061 tattttattt gaaagttgtt cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta
    15121 catgccacaa acttggacga gcgacaatac cgatgccgtg gctcgtctga aaattcaata
    15181 tggaaccagc ttagtgtatc caatttcaag tatgggtgcg catgtttcag cagtcccaaa
    15241 tcatcaaaca ggaagagaaa cgagtttaac tattcgtggc aatgctgcga tggctggtgt
    15301 ttttggttat gagctagatc taacgcaatt aacttctgaa gaaaaagcag aaattaaaga
    15361 acaagttgcc ttttataaaa agcatcgcca acttttgcaa tatggaactt tttatcgctt
    15421 ggaaagtccg tttgaaggca atgatactgc atggatgttt gtctcaaaag accaaacaga
    15481 agccatggtc ttttatttcc gagtattagc agaagctgcg catccattag tcacattgaa
    15541 attagcagga ttagcagaag aagcaactta tcaaatggaa gatgtaacat ttgctggcga
    15601 tgaattgatg aatctcggtt tttatattga ctcagaacta catggtgatt atgcgacaca
    15661 acgcttttat ttgaaacaga tatagtagct tgaatttaca atataagtgc aacaccaaaa
    15721 gagactcatt ccaaacgaag aaaatatgtt ttatttacac gcatttacta aaaagcagcc
    15781 tgatttgaat tgggaaaatg aagaagtacg tgaagaaatc tatgcaatga ttaactattg
    15841 gttagataaa ggactaggcg gatttagaat cgatgcgata ttaaacatca aaaaaaaact
    15901 agagtatggc atctttgaat ctgatggaga agatgggtta gccttcattg ggccctggat
    15961 tcttaatcag tcgggcattg aagtgtggtt aaaagaaatg agagagcgga catttaaacc
    16021 acataatagt atgacggtag cagaagccga tgttcctaat gaacgtttag atgaatatat
    16081 tggtgaagat ggctattatt cgatggtttt tgattttagt tatacggata ttgatgtacc
    16141 tgaaactggt gaatggttta aagatagcca atggacatgg acggatatgc gtaccaatat
    16201 ttttacgaat caattagtta cgcaagataa aggttggggc gccttatatt tagaaaacca
    16261 cgatcaacca cgttcaatta ataaatatat tccagaagaa tggatcaatg attatagtaa
    16321 aaaaatgttg gcgacgcttt ttatgttact aagaggtacc ccttttattt atcaaggaca
    16381 agaactagga atgaccaaca tagaaatggc atcattagaa gattttgatg atgtagctac
    16441 gcattctcag tataaacggg cattagaata tggattggca ccagagcaag cattgaaagc
    16501 tgtcaataaa cgaagtcggg ataattctcg cacacctatg caatggaata cgcaaaaaaa
    16561 tgccagattc tcaacttctg ataatgtatg gttaaaagta aatcctaatt atccagaact
    16621 caatgcagaa gctcagttaa ccaatcaaca gtcggtattc aattattatc atcaattaat
    16681 taaattaaga aaaaatagtg tatacaaaga cgtgctaatc tacggacaat ttctaccagt
    16741 gaaagataaa aatgaacata ttttgcttta tgaacgccaa ttagaagata aaatcgtatt
    16801 agttatctta aacgttacag caacagaaca agaatattat gtagatgaac agtaccaaca
    16861 agtattaatc aataattatg aagatgtttt attagatcaa agtcgaaaat tgcgtttacg
    16921 accgtttgaa agtattgtat tagctaatta tggaggacaa ttatgaaaat taaaaacgaa
    16981 gcaatgctca tcacctatgc agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc
    17041 ttagacaaac atttacaagg tgttattggt ggtgtacatc tgttgccttt ttttccatcg
    17101 acaggtgatc gaggctttgc gccgagtgat tatacgacag ttgatccagc tcttggaaca
    17161 tgggaagatg tggaagcatt aggtgaagat tattatttga tgtttgattt tatgattaat
    17221 cacatttctc gtgaatcaaa atttttccaa gatttcaaaa aaaaccatga gcagtcacca
    17281 tacaaagaaa tgtttatccg tattcatgaa tttttcccag aaaatcgacc aacacaagaa
    17341 gatattgatt taatctacaa gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg
    17401 gatggtacga cagaagaggt ttggaatacc tttggggaag aacaaattga tttagatgtg
    17461 accaaagaag ttgtgaaaga atttattcga gaaaccatca aagatatggc gaaccatggc
    17521 tgttctttaa ttcgattaga tgcgtttgct tatgcgatta aaaaattaga taccaatgat
    17581 ttcttcgtgg aaccagatat ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa
    17641 tatgacgtcg aattattacc agaaatccat gagcattatt ccattcaaat gaaaattgcc
    17701 aatcatgatt attatatcta tgacttcgcc ttgccaatgg tcactctgta ttcactttat
    17761 agtggaaaat cagaacgttt agccaattgg ttgaaaatga gtccgatgaa gcagtttaca
    17821 acattggata cgcatgatgg aattggtgtt gtggacgctc gtgatttatt aacagatgaa
    17881 gaattggatt acacctctga ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat
    17941 tcaagtgcca attacaataa tttagatatt tatcaaatta acagcaccta ttatagtgca
    18001 ttaggcgata atgaccgtag ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg
    18061 attccacaaa tttattacgt tgggttatta gcaggaaaaa atgatattga actgttggaa
    18121 gcaacgaaag aagggcgtaa tattaatcgt cattactatg atttagccga aattgaagaa
    18181 gaagtgcaac ggccagtgat tcaacaatta tttgcacttt taaaatttag aaatgaatct
    18241 gcggcgtttg atttggatgg aacgattgaa gtagcaacgc catcagaatc agaaatagtg
    18301 attacacgta aaaataaaaa cggtagtgat agtgcaacat taacggctaa cttggatacg
    18361 aaagaattta tcattgagtc taaagatata aaaattttca ctagtgttgc attaaaattt
    18421 taacctattc catagaacaa aaaatccttt atactagcac ttggatgact tatccaagac
    18481 caataaaaag gactcataca tggataagta taaaacaaat acagcattta ataaatggtt
    18541 ttctgccata aaattaggaa aactttctgt caactcccaa aaatgtattt ttgattacaa
    18601 ttcgtatcgt aaaaaactgt cttttgaagc tgtgctcaag ctctttctct acgcagttaa
    18661 tgaagaaaaa gaaagtttgc gtgatttgag tacagctctt atcgaccgat cgctccaact
    18721 agaaaccgat gtgtcatcga ttagtcatac ccaattgtct cgagcattta atgcacttga
    18781 acctaaagtg ttagaagaaa tttttcaaca actgttagaa aaagttcaac atcaaatcaa
    18841 accaaccaag cgaaatcatc tgtatctgat tgattccagt acgttttcgc ttagtttaaa
    18901 acgtcataag tgggcgactt tccgtcaaac aaaatcaggt gtcaagttac acttaaacta
    18961 ttgttatatg gacgatacta cgatgtatcc aactgatttt accttaacaa atgcccatga
    19021 acatgatctc aatcaactac cagtattggt gaatcaacca gaggcaactt acgtgtttga
    19081 tcgtgggtat taggactttg aaaagatgga tcagatgcat tgggatggct atttcttcgt
    19141 cactcggatt aaaaaaaaca ccaaagttca cgtgatagat actctggaaa catcgccaga
    19201 aacagaaatt ctacgagacg agctggttcg tttaggatct aaaacctatc taaccgccaa
    19261 tttccgacta gtcactgttc aagataagaa cggacgtgtg tttcaattta tcaccaatcg
    19321 aatggacgtc tcttcaaaag aaatctctga tatgtatcac gctcgttggc aaatcgaatt
    19381 gtttttcaag cacatcaaac aacacatgac cattaaaacc ttcttttctc aaagtgaaaa
    19441 aggcgtgcag aatcaattga ttttaacgat gatttccgct ctcctcacgt ttctcatcaa
    19501 attggagacg aaaacggaaa aatcagtgtt ccaaatcaaa cgattctttc gttatttact
    19561 gtttcagccc tttgaatgtt gtctagaaaa attaattccc acatgagaaa aaagaaattt
    19621 ggctcgttac ttttggtttt cagttgaata acagataaaa aattttctgg aaaaagtcat
    19681 cctctgcgtc agcacagttt tttttgactt attttcagag gaaatagttt tcagaatatt
    19741 ctaaaatgtt ttatgcaaca ctagtgatat tttttagaat tagatttcta atgtgcattt
    19801 tttgagtatt aaggagcaag tatacaaaga tttataaagc tgattaaatt tgaataaaaa
    19861 tatgcaggaa cgctactatt tttagtagtg ttctttttta tatttgaata aaaaatgtgg
    19921 tggttaaatg gtggttataa caaaaaatga attaatgaaa ttaggttatg ggaagtatca
    19981 gtctgagaga atcatacgac aggcaaaagc aattatggtt tcaaaaagct atacctatta
    20041 cgaaaataga agattaggac tagtacctgt tgaatctgta gaagaagttt taggtatgaa
    20101 gatagaggta gaggaggatt ttcagaatca tggttaattt taaaaaagat aaaaatggga
    20161 aatatcattt tactgctagt ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa
    20221 acgtagaggg gttaaaacag ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat
    20281 tatggaaaag aagcagaaac ttctcaaagt actatgttgt ttgaggagtt tttttataaa
    20341 ttattcctac cttattatca agggaaagta gaagaaagaa catataaaaa tcggttatct
    20401 acaatcaaaa aaacttttct tatttcttta aaatggagtt aagagaaata cgtccaattg
    20461 acattcaaaa gtggcaaaat aatttattga gcaaatataa aaattcttat gtaagaaaca
    20521 tgtatgggat ttttcagatg tcattagatc gagcagttgt tttgggacta cttgaaaaaa
    20581 atcctgcaaa agttattgga aatgtgaaga aaaggaaaac tacagttgat ttttggacaa
    20641 aagaagaatt tacaaaagtt atttcaactt tttatattga tgatttgtac caacattttg
    20701 gttttatttg tatgtggtta ctttttatga ccgttatgcg tttgggagaa gccactgcat
    20761 taacttggaa agatattgat tttgaaaata aaaacttgcg tgtgaataaa acactctatt
    20821 ataagagagc caaaacctat gagtttgtag aacccgaaac ggcttctagt attcgtacaa
    20881 tcgaactaga cgacaataca attacctttt taaaagcttg gaaggagcgt caagtttcaa
    20941 taggaggcat gtcgtttgta ctttcttata atggaacacc tactcagaaa tatacaatcc
    21001 gtcatattat gagaagacat gcaacgatcg ttggtgttca tataatccgc atacacggat
    21061 taaggcatag tcatgcttct ttgcttatat cacaataaaa agatgcactt cgaattaaag
    21121 aaaggttaaa acatgctgat gtacaaacga ctttaggaac ttatggtcat ttgtatctaa
    21181 attcattgag agagataaca aatgatccca cagattatat ccaaactaat ttttctaaaa
    21241 tcaaaccgac acctatttac gataataaaa aataaatgat aaacaaaagt aggatcacac
    21301 caaaagaaac acactagaaa aactataata caggcttttc tagcgtgttg tttcttattt
    21361 ccgcctattt atacattata ctcataaagc atttaatgca tcgatcgtag ccgcattgtc
    21421 tatatgggta tataaagttg ttgtattggt acttgaatgt cccaattgtt gggcagttaa
    21481 taccaatgaa tttgtctgtt ggtagagtct agtagctaat gtatgacgca gtttgtgagg
    21541 gctgacttga attctaaagc cttttgaata ttttgctacc atccgttcga ttgcaatacc
    21601 ggatatacgc ttggcttctc cttggtaaat tgctagaaat aaagcttttt cgttttctgt
    21661 tactttatat cgttctttcc gaatagctag ataattagct aaatattcca ttgcgaatgc
    21721 tgctatattg acagcatctc ttttatttcc ttttctagtt actaccacat tattcttatc
    21781 taaagatagg tcatctaaat taatatttat aacctcggag agtctcaatc cagatcctaa
    21841 aatcagtgca ttgatcgcca ggtctctttc tacatctcgt tgataataga tcaaagctcg
    21901 tttactcaaa gtatttttat attcattggc aatataatct agataatttt gtgtatcctt
    21961 atctagaaat aattttttct caatggcatt ggcacgtgca gctaacgtag ttttatctgt
    22021 gatggttgct acttgatcca taggattatt gtctaagtat ggttttccta gaggattatt
    22081 ggaacttttc gaaagatatt taaatagaac ctttaatgcc gtgatatttc tattgatagt
    22141 agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg tctctttttt
    22201 tacattttct tttgtgcgac gtttcaaatg agatttgtac aagtttaggt cataggcagt
    22261 taactgtctt atcgaggtga tagaagtatc aggaacgata gactcactca gaagccattc
    22321 ataaaagagc ttataatttt ttagatattc taaagtggtt cgaggtgatt gatctttatc
    22381 ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga ttgtatctat
    22441 tttttgatat atttctgtat ttttcatgtc attcatcctt tcaataagta ggggagaaga
    22501 gtatctctat aattggacta taccaattat agaatgtttt gtcaattggt atgctctaaa
    22561 atttattaat ttgtttcaaa aaaagcatat aaacaggtat acgtttaata atttaaaata
    22621 aatggtatac ataaataggg aaggaaatag aagggagcaa tctaaatttt ttgaattttc
    22681 ttaattaatt atatagaaag aacaatcttt ctatagagtt agacatgaga gggactatgc
    22741 ttgatttaaa atcaacaagc gtatatactg tatatacaaa gaaaaaggag ggagaagact
    22801 atggcaatta aagaaagaaa atttagggaa gtggggaact cagtagtatt aactatttca
    22861 aaggagtttc ttaaagaaaa tgggttaaat cccggagata cggttttttt agatgaagaa
    22921 aaacttcgag cggcaattac aaaaaaacaa gccttatctg atgaaattga tttagcaata
    22981 tctcaagcat taactgaata tgatactact tttagagagc ttgtcgataa atgagtattc
    23041 attacctaga agccaaagat attataaaaa tgaatgtaat ccaaataaaa aaatactctc
    23101 ctaatgaacc ccttggtgta aaggatgcga atgctttaga aatgtgtgta gggcaacttc
    23161 gagcctcagc ttttggtgaa gaggtttatc cttcgattta tgaaaaagca gctatcctac
    23221 ttatacaatt gataaagaaa catccttttc acaatgcaaa taaacgtact gctttcttag
    23281 ctacttttgt ctttttgaaa atcaatggac agcttttagc tattgatcaa aaagatgctg
    23341 taaatttagc ggtatatatt gctacgtatg ataaggattt cgatcaacta aaatatgaag
    23401 tgatcaacac tataaagaca catacgcagc ccatttaagg agttactgta tgtgtcttta
    23461 tagggttatt agctgctata gcgaaataat tgaagtaaat tacaaatagc tataattcaa
    23521 atccaactat atagaaaacg taaattttct atatagttgg attaagataa cgactattta
    23581 ttttatcata tttttactat accctatact tttacttggt atatttatga ataatcgcaa
    23641 atttacccct atactactag tttgtaagtt agattaataa aaatactaaa gttatttgtt
    23701 cttttattaa atacacctac aacttataaa ttgatggtat ttagataaca cgaaagaata
    23761 aaaataacag ctgatagtaa agtttgttga cgattgctac tcaatcgaaa gtgagttagt
    23821 gcgtatgaac ttgcttctga aaacttatga aatgaaagac cttttacatc ttgcagaaat
    23881 cggtactggg attaaactaa gcaataaaaa ctaatgttcc cctcattctt tgaacttcta
    23941 ctatttgatt tatttattct gataaatgac gatttcacct tcaggatctt caaatcccgt
    24001 atagaattta tttaagatct cttcagtatc agtaatagga taggcgatag gccacatacc
    24061 ctttgatggt tagaataaac tgctcaaaat ctgtatcgta tgttggcttt agcatattaa
    24121 caaaggctat ctccaaatta taaaccataa gcgttgaatc gttcttaaca atttcaattc
    24181 ttcctaaccc aaagtcagaa gatataatta ccggacttgc cttacccttc atgtgggttg
    24241 ttgagattcc atatttaaag ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa
    24301 aaataaaaaa atctttcccc tgctagtctc ctaagtccct ttgtagcgga tatggcacca
    24361 taatctcatt ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa attgatacaa
    24421 tctaacgatg caccctctac ggcattctag gggagtgcac ttgcattcac ccaataaaaa
    24481 ccggcagtta gcaaagaact tatgtagtcg tatgttctta ctgttaacat acaagaggtc
    24541 tcctttccac aattgcttag cagtaaacgg gaatttacaa agctaatctc ttccattttc
    24601 ttatctttgt tctaaaagtc ccaaatggag caactgtatt tacatgaata aatttatata
    24661 cttcccatac agccgttttg gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata
    24721 gatcttcatc actcattgaa tcaatcatca tgttaatatc atcaatatta cgattgagaa
    24781 tttcctctaa ttctttcaat ggtagatgtg cataggtatt tgtaaaccac ttatataact
    24841 ctcctaactg attccattta aattgatcag aaggcgtttt aacagataat ccatttcttt
    24901 catcattttc ccattttaga attaaattcg tccaaccaac ttgatatgct aaattttcag
    24961 caggtgttct atccacttcc tcaactcttt tatccttcaa ttcttcagga atagtttcaa
    25021 attctaaaat atatttagta taagtcctat ttatttcttt ttttaattct tctttatttt
    25081 tatatgttct cattatatta cctccataaa ttcctaattg tctcctttaa ttcatctata
    25141 atttcatcca cgatatttta atccgttcta caatgcatat ctgatatgca ttgactgtct
    25201 aactcatatg tgaatgattt ataaaatatc ccaacgtctc atctaaagaa atgttgatcg
    25261 tcagcaagga gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg actggtttgc
    25321 atatcatttt cttcgtaaat atcaaaatca tgttaactac tatcaattac taataaaaac
    25381 taccacttta agcgttaaga actgttaagt ttagttaagt gaggatagtt cacaaaatcc
    25441 attgttaaac ttcacgaaag tggtaaaact caaaatgctt tatataggat tacagcatcg
    25501 ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg acgatgacac
    25561 gttccttacg gctcatcaga tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa
    25621 tttaattcta aaaaagcgat tgccatattc acgcctcgct cgacgaacta ttaaaggtca
    25681 agatttacct gaagatcatc ctttttagga aattgaagct ttttatgttg cacaatgcat
    25741 ttataatact acaatgatgt tttcaccaga tattcttcta gggtgcggtg taatgaaaca
    25801 gatacacttg caaaagaaaa ttcaccgcga atttactaaa attatgaata attatgttaa
    25861 tcttccgaaa tttttttcca aaattaaggg atcaagctgg tgttatagat tgtttaaggt
    25921 tggcaaaaaa ataacttctt tttaaacaat tatagaaaac cgatactctg ttaagttggt
    25981 tgacacggtg tcgattttta tgactttctt attctgaaac ttcactaacg gacagtacct
    26041 aaaaagtcta tagtatggac gtttacgagt actattttat caaaaatgct gctcatttca
    26101 acttatatgt tttagtgaac tacccccact ta
//
LOCUS       DAFCNJ010000036        25194 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000036 DAFCNJ010000000
VERSION     DAFCNJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.037"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..746
                     /locus_tag="LNE35_001796"
     CDS             99..746
                     /locus_tag="LNE35_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK6910937.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="LNE35_001797"
     CDS             1139..3784
                     /locus_tag="LNE35_001797"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBK6910938.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="LNE35_001798"
     CDS             4094..5407
                     /locus_tag="LNE35_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBK6910939.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="LNE35_001799"
     CDS             5397..6050
                     /locus_tag="LNE35_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6910940.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="LNE35_001800"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="LNE35_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="LNE35_001801"
     CDS             6787..7872
                     /locus_tag="LNE35_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6910941.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="LNE35_001802"
     CDS             7869..9761
                     /locus_tag="LNE35_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6910942.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="LNE35_001803"
     CDS             9768..10145
                     /locus_tag="LNE35_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6910943.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="LNE35_001804"
     CDS             10299..11081
                     /locus_tag="LNE35_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HBK6910944.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="LNE35_001805"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="LNE35_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HBK6910945.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12055)
                     /locus_tag="LNE35_001806"
     CDS             complement(11996..12055)
                     /locus_tag="LNE35_001806"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HBK6910946.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12447..13109
                     /locus_tag="LNE35_001807"
     CDS             12447..13109
                     /locus_tag="LNE35_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6910947.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13344..13487
                     /locus_tag="LNE35_001808"
                     /pseudo
     CDS             13344..13487
                     /locus_tag="LNE35_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13637..13837
                     /locus_tag="LNE35_001809"
     CDS             13637..13837
                     /locus_tag="LNE35_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6910948.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14091..15770)
                     /locus_tag="LNE35_001810"
     CDS             complement(14091..15770)
                     /locus_tag="LNE35_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK6910949.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15772..15984)
                     /locus_tag="LNE35_001811"
     CDS             complement(15772..15984)
                     /locus_tag="LNE35_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBK6910950.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16377..18107
                     /gene="efrA"
                     /locus_tag="LNE35_001812"
     CDS             16377..18107
                     /gene="efrA"
                     /locus_tag="LNE35_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBK6910951.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18104..19864
                     /gene="efrB"
                     /locus_tag="LNE35_001813"
     CDS             18104..19864
                     /gene="efrB"
                     /locus_tag="LNE35_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBK6910952.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19959..20156
                     /locus_tag="LNE35_001814"
     CDS             19959..20156
                     /locus_tag="LNE35_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910953.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20309..20794
                     /locus_tag="LNE35_001815"
     CDS             20309..20794
                     /locus_tag="LNE35_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBK6910954.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="LNE35_001816"
     CDS             complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="LNE35_001816"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBK6910955.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21671..22198
                     /locus_tag="LNE35_001817"
     CDS             21671..22198
                     /locus_tag="LNE35_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6910956.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22289..23026
                     /locus_tag="LNE35_001818"
     CDS             22289..23026
                     /locus_tag="LNE35_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK6910957.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23030..23947
                     /gene="miaA"
                     /locus_tag="LNE35_001819"
     CDS             23030..23947
                     /gene="miaA"
                     /locus_tag="LNE35_001819"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBK6910958.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23949..25178
                     /gene="hflX"
                     /locus_tag="LNE35_001820"
     CDS             23949..25178
                     /gene="hflX"
                     /locus_tag="LNE35_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBK6910959.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8390 a         4103 c         5297 g         7404 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
    12241 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
    12301 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
    12361 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
    12421 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
    12481 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
    12541 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
    12601 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
    12661 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
    12721 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
    12781 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
    12841 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
    12901 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
    12961 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
    13021 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
    13081 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
    13141 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc
    13201 ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag
    13261 taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg
    13321 taatttactc ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga
    13381 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    13441 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    13501 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    13561 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    13621 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    13681 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    13741 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    13801 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    13861 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
    13921 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
    13981 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
    14041 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
    14101 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
    14161 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
    14221 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
    14281 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
    14341 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
    14401 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
    14461 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
    14521 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
    14581 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
    14641 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
    14701 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
    14761 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
    14821 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
    14881 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
    14941 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
    15001 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
    15061 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
    15121 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
    15181 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
    15241 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
    15301 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
    15361 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
    15421 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
    15481 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
    15541 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
    15601 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
    15661 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
    15721 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
    15781 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
    15841 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
    15901 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
    15961 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
    16021 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
    16081 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
    16141 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
    16201 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
    16261 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
    16321 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
    16381 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
    16441 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
    16501 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
    16561 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    16621 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    16681 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    16741 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    16801 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    16861 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    16921 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    16981 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    17041 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    17101 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    17161 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    17221 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    17281 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    17341 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    17401 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    17461 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    17521 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    17581 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    17641 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    17701 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    17761 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    17821 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    17881 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    17941 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    18001 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    18061 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    18121 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    18181 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    18241 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    18301 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    18361 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    18421 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    18481 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    18541 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    18601 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    18661 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    18721 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    18781 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    18841 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    18901 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    18961 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    19021 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    19081 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    19141 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    19201 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    19261 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    19321 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    19381 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    19441 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    19501 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    19561 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    19621 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    19681 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    19741 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    19801 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    19861 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    19921 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    19981 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    20041 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    20101 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    20161 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    20221 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    20281 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    20341 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    20401 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    20461 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    20521 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    20581 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    20641 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    20701 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    20761 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    20821 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    20881 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    20941 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    21001 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    21061 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    21121 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    21181 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    21241 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    21301 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    21361 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    21421 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    21481 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    21541 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    21601 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    21661 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    21721 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    21781 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    21841 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    21901 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    21961 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    22021 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    22081 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    22141 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    22201 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    22261 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    22321 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    22381 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    22441 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    22501 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    22561 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    22621 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    22681 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    22741 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    22801 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    22861 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    22921 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    22981 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    23041 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    23101 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    23161 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    23221 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    23281 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    23341 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    23401 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    23461 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    23521 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    23581 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    23641 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    23701 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    23761 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    23821 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    23881 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    23941 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    24001 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    24061 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    24121 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    24181 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    24241 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    24301 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    24361 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    24421 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    24481 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    24541 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    24601 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    24661 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    24721 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    24781 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    24841 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    24901 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    24961 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    25021 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    25081 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    25141 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DAFCNJ010000037        24575 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000037 DAFCNJ010000000
VERSION     DAFCNJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.038"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(152..550)
                     /locus_tag="LNE35_001821"
     CDS             complement(152..550)
                     /locus_tag="LNE35_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBK6910960.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="LNE35_001822"
     CDS             complement(547..1320)
                     /locus_tag="LNE35_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK6910961.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="LNE35_001823"
     CDS             complement(1317..2000)
                     /locus_tag="LNE35_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK6910962.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="LNE35_001824"
     CDS             complement(2167..3054)
                     /locus_tag="LNE35_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6910963.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="LNE35_001825"
     CDS             complement(3051..3920)
                     /locus_tag="LNE35_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6910964.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="LNE35_001826"
     CDS             complement(4057..5343)
                     /locus_tag="LNE35_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6910965.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="LNE35_001827"
     CDS             5616..6194
                     /locus_tag="LNE35_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6910966.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="LNE35_001828"
     CDS             6194..7123
                     /locus_tag="LNE35_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6910967.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="LNE35_001829"
     CDS             7120..8856
                     /locus_tag="LNE35_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK6910968.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="LNE35_001830"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="LNE35_001830"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBK6910969.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="LNE35_001831"
     CDS             complement(9752..10261)
                     /locus_tag="LNE35_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBK6910970.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="LNE35_001832"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="LNE35_001832"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK6910971.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="LNE35_001833"
     CDS             complement(11562..12653)
                     /locus_tag="LNE35_001833"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBK6910972.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="LNE35_001834"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="LNE35_001834"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBK6910973.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="LNE35_001835"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="LNE35_001835"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBK6910974.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="LNE35_001836"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="LNE35_001836"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBK6910975.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="LNE35_001837"
     CDS             complement(16616..17590)
                     /locus_tag="LNE35_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBK6910976.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="LNE35_001838"
     CDS             complement(17571..19454)
                     /locus_tag="LNE35_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBK6910977.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="LNE35_001839"
     CDS             complement(19472..21697)
                     /locus_tag="LNE35_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK6910978.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="LNE35_001840"
     CDS             21812..22846
                     /locus_tag="LNE35_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6910979.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="LNE35_001841"
     CDS             complement(22913..23926)
                     /locus_tag="LNE35_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6910980.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="LNE35_001842"
     CDS             complement(24004..24192)
                     /locus_tag="LNE35_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBK6910981.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DAFCNJ010000038        23830 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000038 DAFCNJ010000000
VERSION     DAFCNJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.039"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..788)
                     /locus_tag="LNE35_001843"
     CDS             complement(54..788)
                     /locus_tag="LNE35_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK6910982.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="LNE35_001844"
     CDS             1115..1525
                     /locus_tag="LNE35_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBK6910983.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="LNE35_001845"
     CDS             1518..2219
                     /locus_tag="LNE35_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBK6910984.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="LNE35_001846"
     CDS             2219..3805
                     /locus_tag="LNE35_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6910985.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="LNE35_001847"
     CDS             3824..4045
                     /locus_tag="LNE35_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBK6910986.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="LNE35_001848"
     CDS             4042..4803
                     /locus_tag="LNE35_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBK6910987.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="LNE35_001849"
     CDS             5183..5692
                     /locus_tag="LNE35_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK6910988.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="LNE35_001850"
     CDS             complement(5767..6306)
                     /locus_tag="LNE35_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBK6910989.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="LNE35_001851"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="LNE35_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBK6910990.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="LNE35_001852"
     CDS             7319..8170
                     /locus_tag="LNE35_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBK6910991.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="LNE35_001853"
     CDS             8198..8833
                     /locus_tag="LNE35_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBK6910992.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="LNE35_001854"
     CDS             8853..9626
                     /locus_tag="LNE35_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6910993.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="LNE35_001855"
     CDS             10034..10831
                     /locus_tag="LNE35_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBK6910994.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="LNE35_001856"
     CDS             10809..11222
                     /locus_tag="LNE35_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK6910995.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="LNE35_001857"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="LNE35_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK6910996.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="LNE35_001858"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="LNE35_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBK6910997.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="LNE35_001859"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="LNE35_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBK6910998.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="LNE35_001860"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="LNE35_001860"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBK6910999.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="LNE35_001861"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="LNE35_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBK6911000.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="LNE35_001862"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="LNE35_001862"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBK6911001.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="LNE35_001863"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="LNE35_001863"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBK6911002.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="LNE35_001864"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="LNE35_001864"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBK6911003.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="LNE35_001865"
     CDS             21906..22484
                     /locus_tag="LNE35_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6911004.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="LNE35_001866"
     CDS             22536..23018
                     /locus_tag="LNE35_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBK6911005.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="LNE35_001867"
     CDS             23135..23509
                     /locus_tag="LNE35_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911006.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6649 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt
//
LOCUS       DAFCNJ010000039        23063 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000039 DAFCNJ010000000
VERSION     DAFCNJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.040"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(109..564)
                     /locus_tag="LNE35_001868"
     CDS             complement(109..564)
                     /locus_tag="LNE35_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911007.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="LNE35_001869"
     CDS             complement(718..1926)
                     /locus_tag="LNE35_001869"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBK6911008.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="LNE35_001870"
     CDS             complement(2038..2370)
                     /locus_tag="LNE35_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK6911009.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="LNE35_001871"
     CDS             2518..3393
                     /locus_tag="LNE35_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6911010.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="LNE35_001872"
     CDS             complement(3475..4056)
                     /locus_tag="LNE35_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBK6911011.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="LNE35_001873"
     CDS             complement(4062..5321)
                     /locus_tag="LNE35_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK6911012.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="LNE35_001874"
     CDS             complement(5323..6369)
                     /locus_tag="LNE35_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911013.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="LNE35_001875"
     CDS             complement(6604..6879)
                     /locus_tag="LNE35_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBK6911014.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /gene="der"
                     /locus_tag="LNE35_001876"
     CDS             complement(7122..8432)
                     /gene="der"
                     /locus_tag="LNE35_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBK6911015.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="LNE35_001877"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="LNE35_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBK6911016.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="LNE35_001878"
     CDS             complement(9978..10658)
                     /locus_tag="LNE35_001878"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBK6911017.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="LNE35_001879"
     CDS             complement(10707..11342)
                     /locus_tag="LNE35_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6911018.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="LNE35_001880"
     CDS             complement(11408..12811)
                     /locus_tag="LNE35_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBK6911019.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="LNE35_001881"
     CDS             complement(12798..13829)
                     /locus_tag="LNE35_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6911020.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="LNE35_001882"
     CDS             13860..14081
                     /locus_tag="LNE35_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBK6911021.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="LNE35_001883"
     CDS             complement(14246..14983)
                     /locus_tag="LNE35_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBK6911022.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="LNE35_001884"
     CDS             complement(15136..16476)
                     /locus_tag="LNE35_001884"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBK6911023.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="LNE35_001885"
     CDS             complement(16713..18236)
                     /locus_tag="LNE35_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBK6911024.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="LNE35_001886"
     CDS             complement(18614..19225)
                     /locus_tag="LNE35_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6911025.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="LNE35_001887"
     CDS             complement(19555..20271)
                     /locus_tag="LNE35_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK6911026.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="LNE35_001888"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="LNE35_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBK6911027.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="LNE35_001889"
     CDS             complement(20830..21627)
                     /locus_tag="LNE35_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBK6911028.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="LNE35_001890"
     CDS             complement(21597..21968)
                     /locus_tag="LNE35_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK6911029.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="LNE35_001891"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="LNE35_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBK6911030.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6466 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaa
//
LOCUS       DAFCNJ010000040        22458 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000040 DAFCNJ010000000
VERSION     DAFCNJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.041"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..152
                     /locus_tag="LNE35_001892"
     CDS             <1..152
                     /locus_tag="LNE35_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6911031.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="LNE35_001893"
     CDS             complement(230..763)
                     /locus_tag="LNE35_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBK6911032.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="LNE35_001894"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="LNE35_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBK6911033.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="LNE35_001895"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="LNE35_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBK6911034.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="LNE35_001896"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="LNE35_001896"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK6911035.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="LNE35_001897"
     CDS             complement(4488..5066)
                     /locus_tag="LNE35_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBK6911036.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFVHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="LNE35_001898"
     CDS             5388..5858
                     /locus_tag="LNE35_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBK6911037.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="LNE35_001899"
     CDS             complement(5966..7192)
                     /locus_tag="LNE35_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6911038.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="LNE35_001900"
     CDS             complement(7946..8146)
                     /locus_tag="LNE35_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6911039.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="LNE35_001901"
     CDS             complement(8280..8888)
                     /locus_tag="LNE35_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBK6911040.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="LNE35_001902"
     CDS             complement(8973..9443)
                     /locus_tag="LNE35_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBK6911041.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="LNE35_001903"
     CDS             complement(9491..10405)
                     /locus_tag="LNE35_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6911042.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="LNE35_001904"
     CDS             10551..11354
                     /locus_tag="LNE35_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBK6911043.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="LNE35_001905"
     CDS             11532..12479
                     /locus_tag="LNE35_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBK6911044.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="LNE35_001906"
     CDS             complement(12547..12840)
                     /locus_tag="LNE35_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBK6911045.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="LNE35_001907"
     CDS             complement(12868..13533)
                     /locus_tag="LNE35_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBK6911046.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="LNE35_001908"
     CDS             complement(13673..14305)
                     /locus_tag="LNE35_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6911047.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="LNE35_001909"
     CDS             complement(14312..15379)
                     /locus_tag="LNE35_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6911048.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="LNE35_001910"
     CDS             complement(15376..16107)
                     /locus_tag="LNE35_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBK6911049.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="LNE35_001911"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="LNE35_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBK6911050.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="LNE35_001912"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="LNE35_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBK6911051.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="LNE35_001913"
     CDS             18270..19439
                     /locus_tag="LNE35_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK6911052.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..21340)
                     /locus_tag="LNE35_001914"
     CDS             complement(19706..21340)
                     /locus_tag="LNE35_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911053.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            21825..22458
                     /locus_tag="LNE35_001915"
                     /pseudo
     CDS             21825..22458
                     /locus_tag="LNE35_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         6972 a         4369 c         3555 g         7562 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgca caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat
    21001 aatgacatat aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg
    21061 ctaaattagt aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat
    21121 tatttacttg catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag
    21181 attctaaact taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca
    21241 tgtttttaaa tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt
    21301 ttacagaaaa aatttttttc aaattcatat accacctcat gtttttttat attacatata
    21361 gtatcattta tttttttaaa acactagatt actcactata taaaatgtga ttaattttat
    21421 tattaataaa atatttttta tattacaaaa gtagtgtttc aaagttagta gagaaaatgt
    21481 agttatatat ttaaattttt accagaaaga aatgaacacc ttcaattatc taaatctcga
    21541 aaatggttag atgtaatcat caggaaaaac aatcttaaac gaattactat tcatggtctt
    21601 agacacacac atgctagctt gcttttagca agctagtgca aatattaaag atgtgcaaga
    21661 gcgtttaggc cactcttcta ttcaaatcat tatggatcta tatattcata ttacagacaa
    21721 aagaaaagaa gaaaccgtag cgcaattcgc aaaatatagg ctctataatt tatgggactg
    21781 atgaatcaaa attgattctt cagtcccatt gttattttag gtatatgatt cgtacattga
    21841 aagagttgaa aaactatctt ccgaagccgt caaaaaggac cctgccaaaa gtattgatgg
    21901 tggatgaatt tcgttcgcac gcttcaattg tggataaaat gagctttatt tgtgcggatg
    21961 gcgagactgg aaaactcatt gatattttgc ctacacgaaa attgtctcga ttaacacact
    22021 attttagtcg ctgtgcaaat cctgaagcgg tggaatattt agtgacagat atgaacgccg
    22081 cctacttcca actgacaaaa cgtgtcttac caaatgcgaa agtcgtcatt gattgttttc
    22141 atatcatcaa acatttgaat caggcgttca atgattttcg tgtacaagaa atgaaaagat
    22201 tacaccagtt agggcaacga cgggaagccg agaaagtgaa aaagaactgg cgctttcttc
    22261 taaaaaaccg tcaaaaaatc aatcactatg attacaaaac atggcgcagt tttcgtgcac
    22321 ctaaataccc gctactcact gaagcaatga tgattgatcg attgctggca ttttcaacgc
    22381 ctttaaagga cgcctatact tttttccatg agctgaccga agcctttaga gacaaggaat
    22441 cagacttatt ttttacct
//
LOCUS       DAFCNJ010000041        22031 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000041 DAFCNJ010000000
VERSION     DAFCNJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.042"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            367..555
                     /locus_tag="LNE35_001916"
     CDS             367..555
                     /locus_tag="LNE35_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911054.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="LNE35_001917"
     CDS             545..805
                     /locus_tag="LNE35_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911055.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            1138..1710
                     /locus_tag="LNE35_001918"
     CDS             1138..1710
                     /locus_tag="LNE35_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911056.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="LNE35_001919"
     CDS             1711..2022
                     /locus_tag="LNE35_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK6911057.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="LNE35_001920"
     CDS             2013..2273
                     /locus_tag="LNE35_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911058.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="LNE35_001921"
     CDS             2434..2793
                     /locus_tag="LNE35_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911059.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="LNE35_001922"
     CDS             2790..4433
                     /locus_tag="LNE35_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK6911060.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="LNE35_001923"
     CDS             4468..5550
                     /locus_tag="LNE35_001923"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911061.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="LNE35_001924"
     CDS             5594..6766
                     /locus_tag="LNE35_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK6911062.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="LNE35_001925"
     CDS             6753..7457
                     /locus_tag="LNE35_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBK6911063.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="LNE35_001926"
     CDS             7462..8622
                     /locus_tag="LNE35_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK6911064.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="LNE35_001927"
     CDS             8698..8976
                     /locus_tag="LNE35_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBK6911065.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="LNE35_001928"
     CDS             8957..9328
                     /locus_tag="LNE35_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBK6911066.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="LNE35_001929"
     CDS             9344..9748
                     /locus_tag="LNE35_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911067.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="LNE35_001930"
     CDS             9745..10155
                     /locus_tag="LNE35_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911068.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="LNE35_001931"
     CDS             10216..10803
                     /locus_tag="LNE35_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6911069.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="LNE35_001932"
     CDS             10876..11343
                     /locus_tag="LNE35_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HBK6911070.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="LNE35_001933"
     CDS             11389..11697
                     /locus_tag="LNE35_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911071.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="LNE35_001934"
     CDS             11892..18626
                     /locus_tag="LNE35_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK6911072.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="LNE35_001935"
     CDS             18630..19502
                     /locus_tag="LNE35_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HBK6911073.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="LNE35_001936"
     CDS             19499..20548
                     /locus_tag="LNE35_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911074.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="LNE35_001937"
     CDS             20552..21376
                     /locus_tag="LNE35_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911075.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="LNE35_001938"
     CDS             21394..>22031
                     /locus_tag="LNE35_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBK6911076.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DAFCNJ010000042        22026 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000042 DAFCNJ010000000
VERSION     DAFCNJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.043"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(61..1371)
                     /locus_tag="LNE35_001939"
     CDS             complement(61..1371)
                     /locus_tag="LNE35_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK6911077.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="LNE35_001940"
     CDS             complement(1383..2801)
                     /locus_tag="LNE35_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6911078.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="LNE35_001941"
     CDS             complement(3041..4609)
                     /locus_tag="LNE35_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK6911079.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="LNE35_001942"
     CDS             complement(4613..4894)
                     /locus_tag="LNE35_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBK6911080.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="LNE35_001943"
     CDS             complement(4891..5814)
                     /locus_tag="LNE35_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBK6911081.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="LNE35_001944"
     CDS             complement(5811..6689)
                     /locus_tag="LNE35_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBK6911082.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="LNE35_001945"
     CDS             complement(6676..8013)
                     /locus_tag="LNE35_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK6911083.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="LNE35_001946"
     CDS             complement(8016..9119)
                     /locus_tag="LNE35_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK6911084.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="LNE35_001947"
     CDS             complement(9193..10701)
                     /locus_tag="LNE35_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6911085.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="LNE35_001948"
     CDS             complement(10667..11359)
                     /locus_tag="LNE35_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK6911086.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="LNE35_001949"
     CDS             complement(11381..12355)
                     /locus_tag="LNE35_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6911087.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="LNE35_001950"
     CDS             12655..13527
                     /locus_tag="LNE35_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6911088.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="LNE35_001951"
     CDS             complement(14109..16307)
                     /locus_tag="LNE35_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6911089.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="LNE35_001952"
     CDS             16539..17531
                     /locus_tag="LNE35_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6911090.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="LNE35_001953"
     CDS             17591..19012
                     /locus_tag="LNE35_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBK6911091.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="LNE35_001954"
     CDS             19005..20504
                     /locus_tag="LNE35_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBK6911092.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="LNE35_001955"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="LNE35_001955"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBK6911093.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DAFCNJ010000043        21988 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000043 DAFCNJ010000000
VERSION     DAFCNJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.044"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..395
                     /locus_tag="LNE35_001956"
     CDS             <1..395
                     /locus_tag="LNE35_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911094.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="LNE35_001957"
     CDS             398..1147
                     /locus_tag="LNE35_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6911095.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="LNE35_001958"
     CDS             1159..1428
                     /locus_tag="LNE35_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911096.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="LNE35_001959"
     CDS             1433..1597
                     /locus_tag="LNE35_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911097.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="LNE35_001960"
     CDS             1590..1892
                     /locus_tag="LNE35_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911098.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="LNE35_001961"
     CDS             1889..2050
                     /locus_tag="LNE35_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK6911099.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="LNE35_001962"
     CDS             2047..2352
                     /locus_tag="LNE35_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911100.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="LNE35_001963"
     CDS             2352..2708
                     /locus_tag="LNE35_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBK6911101.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="LNE35_001964"
     CDS             2695..2913
                     /locus_tag="LNE35_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911102.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="LNE35_001965"
     CDS             2910..3329
                     /locus_tag="LNE35_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBK6911103.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="LNE35_001966"
     CDS             3326..3883
                     /locus_tag="LNE35_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBK6911104.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="LNE35_001967"
     CDS             3880..4176
                     /locus_tag="LNE35_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911105.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="LNE35_001968"
     CDS             4253..4666
                     /locus_tag="LNE35_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK6911106.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="LNE35_001969"
     CDS             5012..5386
                     /locus_tag="LNE35_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911107.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="LNE35_001970"
     CDS             5423..5590
                     /locus_tag="LNE35_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911108.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="LNE35_001971"
     CDS             5616..5960
                     /locus_tag="LNE35_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK6911109.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="LNE35_001972"
     CDS             5965..6246
                     /locus_tag="LNE35_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911110.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="LNE35_001973"
     CDS             6349..6663
                     /locus_tag="LNE35_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBK6911111.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="LNE35_001974"
     CDS             6641..8335
                     /locus_tag="LNE35_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK6911112.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="LNE35_001975"
     CDS             8355..9533
                     /locus_tag="LNE35_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK6911113.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="LNE35_001976"
     CDS             9496..10182
                     /locus_tag="LNE35_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBK6911114.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="LNE35_001977"
     CDS             10182..11342
                     /locus_tag="LNE35_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK6911115.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="LNE35_001978"
     CDS             11352..12227
                     /locus_tag="LNE35_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911116.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="LNE35_001979"
     CDS             12224..12535
                     /locus_tag="LNE35_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911117.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="LNE35_001980"
     CDS             12525..12878
                     /locus_tag="LNE35_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK6911118.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="LNE35_001981"
     CDS             12868..13269
                     /locus_tag="LNE35_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911119.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="LNE35_001982"
     CDS             13262..13666
                     /locus_tag="LNE35_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911120.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="LNE35_001983"
     CDS             13678..14286
                     /locus_tag="LNE35_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6911121.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="LNE35_001984"
     CDS             14306..14668
                     /locus_tag="LNE35_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911122.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="LNE35_001985"
     CDS             14671..14853
                     /locus_tag="LNE35_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911123.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="LNE35_001986"
     CDS             14870..18289
                     /locus_tag="LNE35_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK6911124.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="LNE35_001987"
     CDS             18340..19077
                     /locus_tag="LNE35_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6911125.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="LNE35_001988"
     CDS             19087..21378
                     /locus_tag="LNE35_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6911126.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="LNE35_001989"
     CDS             21402..>21988
                     /locus_tag="LNE35_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911127.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAFCNJ010000044        21911 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000044 DAFCNJ010000000
VERSION     DAFCNJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.045"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="LNE35_001990"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="LNE35_001990"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBK6911128.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="LNE35_001991"
     CDS             complement(1172..1867)
                     /locus_tag="LNE35_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBK6911129.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="LNE35_001992"
     CDS             complement(1880..2596)
                     /locus_tag="LNE35_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBK6911130.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="LNE35_001993"
     CDS             2926..3534
                     /locus_tag="LNE35_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBK6911131.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="LNE35_001994"
     CDS             3859..4182
                     /locus_tag="LNE35_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBK6911132.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="LNE35_001995"
     CDS             4364..5734
                     /locus_tag="LNE35_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBK6911133.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="LNE35_001996"
     CDS             complement(5864..7165)
                     /locus_tag="LNE35_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK6911134.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="LNE35_001997"
     CDS             complement(7519..9477)
                     /locus_tag="LNE35_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBK6911135.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="LNE35_001998"
     CDS             complement(9654..9920)
                     /locus_tag="LNE35_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6911136.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="LNE35_001999"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="LNE35_001999"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBK6911137.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="LNE35_002000"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="LNE35_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBK6911138.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="LNE35_002001"
     CDS             13264..13827
                     /locus_tag="LNE35_002001"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK6911139.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="LNE35_002002"
     CDS             complement(14080..14601)
                     /locus_tag="LNE35_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBK6911140.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="LNE35_002003"
     CDS             complement(14604..15446)
                     /locus_tag="LNE35_002003"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBK6911141.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="LNE35_002004"
     CDS             complement(15439..16164)
                     /locus_tag="LNE35_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBK6911142.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="LNE35_002005"
     CDS             16340..16636
                     /locus_tag="LNE35_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911143.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="LNE35_002006"
     CDS             17204..18796
                     /locus_tag="LNE35_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6911144.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="LNE35_002007"
     CDS             18809..19549
                     /locus_tag="LNE35_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6911145.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="LNE35_002008"
     CDS             19717..21066
                     /locus_tag="LNE35_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6911146.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="LNE35_002009"
     CDS             complement(21194..21784)
                     /locus_tag="LNE35_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK6911147.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6294 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa a
//
LOCUS       DAFCNJ010000045        20595 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000045 DAFCNJ010000000
VERSION     DAFCNJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.046"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..293
                     /locus_tag="LNE35_002010"
     CDS             99..293
                     /locus_tag="LNE35_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911148.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="LNE35_002011"
     CDS             562..861
                     /locus_tag="LNE35_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911149.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="LNE35_002012"
     CDS             complement(958..1392)
                     /locus_tag="LNE35_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6911150.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="LNE35_002013"
     CDS             complement(1624..2334)
                     /locus_tag="LNE35_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK6911151.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="LNE35_002014"
     CDS             complement(2324..3184)
                     /locus_tag="LNE35_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBK6911152.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="LNE35_002015"
     CDS             complement(3189..3827)
                     /locus_tag="LNE35_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBK6911153.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="LNE35_002016"
     CDS             complement(3842..4141)
                     /locus_tag="LNE35_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6911154.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="LNE35_002017"
     CDS             complement(4184..5668)
                     /locus_tag="LNE35_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK6911155.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="LNE35_002018"
     CDS             complement(5688..6149)
                     /locus_tag="LNE35_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6911156.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="LNE35_002019"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="LNE35_002019"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBK6911157.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="LNE35_002020"
     CDS             7452..8222
                     /locus_tag="LNE35_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6911158.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="LNE35_002021"
     CDS             complement(8495..9322)
                     /locus_tag="LNE35_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6911159.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="LNE35_002022"
     CDS             9890..11125
                     /locus_tag="LNE35_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK6911160.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="LNE35_002023"
     CDS             complement(12124..12384)
                     /locus_tag="LNE35_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK6911161.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="LNE35_002024"
     CDS             complement(12411..13712)
                     /locus_tag="LNE35_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6911162.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="LNE35_002025"
     CDS             complement(14004..14462)
                     /locus_tag="LNE35_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6911163.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15806)
                     /locus_tag="LNE35_002026"
     CDS             complement(14475..15806)
                     /locus_tag="LNE35_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBK6911164.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17259)
                     /locus_tag="LNE35_002027"
     CDS             complement(15817..17259)
                     /locus_tag="LNE35_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6911165.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="LNE35_002028"
     CDS             complement(17246..18010)
                     /locus_tag="LNE35_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBK6911166.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="LNE35_002029"
     CDS             complement(18114..18989)
                     /locus_tag="LNE35_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6911167.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="LNE35_002030"
     tRNA            complement(19244..19328)
                     /locus_tag="LNE35_002030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="LNE35_002031"
     tRNA            complement(19346..19417)
                     /locus_tag="LNE35_002031"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="LNE35_002032"
     tRNA            complement(19427..19499)
                     /locus_tag="LNE35_002032"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="LNE35_002033"
     tRNA            complement(19561..19645)
                     /locus_tag="LNE35_002033"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="LNE35_002034"
     tRNA            complement(19666..19736)
                     /locus_tag="LNE35_002034"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
     gene            complement(19782..19853)
                     /locus_tag="LNE35_002035"
     tRNA            complement(19782..19853)
                     /locus_tag="LNE35_002035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19819..19821),aa:Gln,seq:ttg)
     gene            complement(19865..19937)
                     /locus_tag="LNE35_002036"
     tRNA            complement(19865..19937)
                     /locus_tag="LNE35_002036"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19902..19904),aa:His,seq:gtg)
     gene            complement(19940..20013)
                     /locus_tag="LNE35_002037"
     tRNA            complement(19940..20013)
                     /locus_tag="LNE35_002037"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19979..19981),aa:Trp,seq:cca)
     gene            complement(20020..20100)
                     /locus_tag="LNE35_002038"
     tRNA            complement(20020..20100)
                     /locus_tag="LNE35_002038"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20064..20066),aa:Tyr,seq:gta)
     gene            complement(20102..20177)
                     /locus_tag="LNE35_002039"
     tRNA            complement(20102..20177)
                     /locus_tag="LNE35_002039"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20142..20144),aa:Phe,seq:gaa)
     gene            complement(20213..20285)
                     /locus_tag="LNE35_002040"
     tRNA            complement(20213..20285)
                     /locus_tag="LNE35_002040"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20250..20252),aa:Asp,seq:gtc)
     gene            complement(20295..20367)
                     /locus_tag="LNE35_002041"
     tRNA            complement(20295..20367)
                     /locus_tag="LNE35_002041"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20332..20334),aa:Val,seq:tac)
     gene            complement(20383..20454)
                     /locus_tag="LNE35_002042"
     tRNA            complement(20383..20454)
                     /locus_tag="LNE35_002042"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20419..20421),aa:Glu,seq:ttc)
     gene            complement(20469..20558)
                     /locus_tag="LNE35_002043"
     tRNA            complement(20469..20558)
                     /locus_tag="LNE35_002043"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20522..20524),aa:Ser,seq:gga)
BASE COUNT         6501 a         4312 c         3269 g         6513 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatagaa actggggtag ctggattcga
    19801 accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa
    19861 aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac
    19921 cacttcgcca cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg
    19981 gagaccgctg ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac
    20041 cactgaaccc taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc
    20101 atggtggcgc gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc
    20161 aactgagcta ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg
    20221 acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg
    20281 gaaccagttc attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag
    20341 atgctctccc agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg
    20401 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa
    20461 atttgctacg gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat
    20521 ttccaatcat gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact
    20581 ctttctcaaa atgag
//
LOCUS       DAFCNJ010000046        20485 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000046 DAFCNJ010000000
VERSION     DAFCNJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.047"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="LNE35_002044"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="LNE35_002044"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBK6911168.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="LNE35_002045"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="LNE35_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBK6911169.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="LNE35_002046"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="LNE35_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBK6911170.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="LNE35_002047"
     CDS             2857..3732
                     /locus_tag="LNE35_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBK6911171.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="LNE35_002048"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="LNE35_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBK6911172.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="LNE35_002049"
     CDS             4629..5063
                     /locus_tag="LNE35_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBK6911173.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="LNE35_002050"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="LNE35_002050"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBK6911174.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="LNE35_002051"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="LNE35_002051"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBK6911175.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="LNE35_002052"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="LNE35_002052"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBK6911176.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="LNE35_002053"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="LNE35_002053"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBK6911177.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="LNE35_002054"
     CDS             13334..14260
                     /locus_tag="LNE35_002054"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBK6911178.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="LNE35_002055"
     CDS             14442..15335
                     /locus_tag="LNE35_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBK6911179.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="LNE35_002056"
     CDS             15469..15594
                     /locus_tag="LNE35_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6911180.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="LNE35_002057"
     CDS             complement(15728..16360)
                     /locus_tag="LNE35_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911181.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="LNE35_002058"
     CDS             complement(16508..17050)
                     /locus_tag="LNE35_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBK6911182.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="LNE35_002059"
     CDS             17185..17574
                     /locus_tag="LNE35_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911183.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="LNE35_002060"
     CDS             17578..18342
                     /locus_tag="LNE35_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6911184.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="LNE35_002061"
     CDS             18471..19418
                     /locus_tag="LNE35_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6911185.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="LNE35_002062"
     CDS             19548..20288
                     /locus_tag="LNE35_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911186.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5495 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtt
//
LOCUS       DAFCNJ010000047        19686 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000047 DAFCNJ010000000
VERSION     DAFCNJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.048"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            315..1094
                     /locus_tag="LNE35_002063"
     CDS             315..1094
                     /locus_tag="LNE35_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911187.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1140..2084
                     /locus_tag="LNE35_002064"
     CDS             1140..2084
                     /locus_tag="LNE35_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6911188.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2100..2879
                     /locus_tag="LNE35_002065"
     CDS             2100..2879
                     /locus_tag="LNE35_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBK6911189.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2891..3589
                     /locus_tag="LNE35_002066"
     CDS             2891..3589
                     /locus_tag="LNE35_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBK6911190.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3617..4381
                     /locus_tag="LNE35_002067"
     CDS             3617..4381
                     /locus_tag="LNE35_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBK6911191.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4425..5876
                     /locus_tag="LNE35_002068"
     CDS             4425..5876
                     /locus_tag="LNE35_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK6911192.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5908..7044
                     /locus_tag="LNE35_002069"
     CDS             5908..7044
                     /locus_tag="LNE35_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6911193.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7044..8150
                     /locus_tag="LNE35_002070"
     CDS             7044..8150
                     /locus_tag="LNE35_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBK6911194.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8156..9178
                     /locus_tag="LNE35_002071"
     CDS             8156..9178
                     /locus_tag="LNE35_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HBK6911195.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9200..10255
                     /locus_tag="LNE35_002072"
     CDS             9200..10255
                     /locus_tag="LNE35_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6911196.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            10873..12108
                     /locus_tag="LNE35_002073"
     CDS             10873..12108
                     /locus_tag="LNE35_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911197.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            12192..13280
                     /locus_tag="LNE35_002074"
     CDS             12192..13280
                     /locus_tag="LNE35_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911198.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            13277..14698
                     /locus_tag="LNE35_002075"
     CDS             13277..14698
                     /locus_tag="LNE35_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBK6911199.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            14734..15450
                     /locus_tag="LNE35_002076"
     CDS             14734..15450
                     /locus_tag="LNE35_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBK6911200.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            15469..17052
                     /locus_tag="LNE35_002077"
     CDS             15469..17052
                     /locus_tag="LNE35_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HBK6911201.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            17187..17765
                     /locus_tag="LNE35_002078"
     CDS             17187..17765
                     /locus_tag="LNE35_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911202.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            17711..18229
                     /locus_tag="LNE35_002079"
     CDS             17711..18229
                     /locus_tag="LNE35_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911203.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            18237..19277
                     /locus_tag="LNE35_002080"
     CDS             18237..19277
                     /locus_tag="LNE35_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBK6911204.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            19526..>19686
                     /locus_tag="LNE35_002081"
     CDS             19526..>19686
                     /locus_tag="LNE35_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6911205.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         7181 a         2484 c         3844 g         6177 t
ORIGIN      
        1 taactattat taattcaatg ttaatttttt tgttattcta ttttttgtgt aacttttaaa
       61 aacacctatg aaaattatgg aaataaaaat gaaatcgatt aatttttatc ttattaatgt
      121 tttatttact tttaaatcgt aaattgccct agtcaagaaa acagtttgtg gtatgattat
      181 atagggaaaa cttgtttaca aatatttctg tgaatatatt agtttaatta tgttttttta
      241 aataaaaagc tgtcattttt atgtaagtga attatatctt aatttatcta tcatcaatac
      301 ttaggagtga atctatggaa aagccattcg tcaagtcatt gactgctgtg gcggtaggtg
      361 caggtgctat tgcgatctgt ttgtttggat accatatcaa taatcagcgg caacatcagc
      421 aacggatcaa ttatgcagaa tctgccatca ccaatcaaaa agacaccttg acttctttat
      481 caaaagaagt agacaagctg tatagtacaa aagaaaaaat ttttctaaac cctgaaataa
      541 cagaagaaac aatcacaagt ttgtcaaata agctatcctc tgtcaaactt tcagcagatg
      601 attacgacat caaagaatca gaattgccta aagaagctgt tgcaatccaa gatgagaaga
      661 aagcaatcgt gacacagtta gatagtgcag aatcaaaact gaagatccag acagctgtca
      721 acgatttatt tactaaaaat gtatcaaact ggcagcaagc tgtagacgat gtcatcatca
      781 aagaaaaggt gacaagtgca gatgtcgctc gtgtacgtga aaatatgagc ttttttaaag
      841 atagtccttg gaaaacggtt gttatgcaat atctaggatt cgctgatatt cagatcgcgc
      901 aagtcacgca actagatcag ttattcgata cgatgttaaa ggatggtcaa gtgactgcca
      961 ccgctactta cgatcaatat ttgacggtgc tcagtcaaat agagcagatc cggaatgaaa
     1021 aaataagcgc agcatacgca acaaaagcac aaactgttgc tgagcaaatg ggctatagca
     1081 atataagcta ttgacagtta aaacgttgat tcaagcaagg ttagcaaacg gaggaaacta
     1141 tggcaagacg aagaagaaaa tcaagaggga aaaaaatatt tctgattatt ttagcagttc
     1201 ttatcgtatt agtattagca attgtaggag taggagcaaa attatatctg gatctctcac
     1261 attctattga gaaaacttac gaatcagtgg aacgaaccca aggagaacga acgaaaccag
     1321 ttgatctgga caaacaagaa tctttctctg ttctattgat ggggattgat actggagatt
     1381 taggtcgggt cgatcaagga agatcagata cgatgatggt tgccacagtc agtcctgaag
     1441 acaaacagac aacgatcgtc agcattcctc gtgatacgta tgtcgacatc gtaggtcgtg
     1501 acacaaaaga caagatcaac cacgcttacg cattcggcgg agctgccatg gcgatggata
     1561 ccgtacaaaa atatgtagac atcccaatcg atcactatgt ctctatcaat atgaaagggc
     1621 taaaggaact cgttgatgca gttggtggta tcgaagtcaa taatgacctc actttctcac
     1681 aagatggcta tgactttcca atcggtaaaa ttacattgaa cggggatcag gcattatctt
     1741 attctcgtat gcgttatgaa gacccaaatg gtgattacgg tagacaaggg cgccaaagaa
     1801 agatcgtaga aggcgtagcg aaaaaagtat tgagtctaaa cggtgtcagc aactatcaat
     1861 ccgttttgaa tgctctagag tcgaatatga aaacagacat gaactttgat gaaatgaaaa
     1921 aaattgcttt agattatcgg gaggcatttg gtacgattaa gcaagatcag ctgcaaggag
     1981 aaggcttcat gcaagataat atctcctatc aaaaagtcag tgatcaagaa cttgatcgtg
     2041 tgaaaaaaga attaaaagat caaatgaatc tagagaataa ataggaaagt ggagaaaaaa
     2101 tggaagagac aatcagcttg aaagaaattt ttgaaacatt acgcaaacac ttggtaataa
     2161 tcatcatctc aatgtttgct ggcttggcca tctcaggaat cgtaacattt ttcatcttga
     2221 caccaaaata cagttcacaa gcacaattga tcgtgacact gccacaatca gacactgcaa
     2281 atgtcaatga tgtcaatacg aacctgcaaa tgatcaatac gtataaagat atgatcgtca
     2341 gtgatttagt gctcaatgaa gtcaaagatc gtttagaaac agaagacaat gtgaaaatga
     2401 ctgctgaaca aatcaaagat gccatctcag ttaatcaggc agaaaattca caaatgttct
     2461 cgattcaggc aataagtacg aatgcagtgg ctgctcaaca gatcgctaac acaacttcac
     2521 aagttttcca agagaacgca aaagatgtga tgaatgttga taaaatctcg atcatttcag
     2581 gtgcagttgc caatacgaca cctgtttcgc caaataataa attgaattta atgactggct
     2641 taatgttagg aatgatggta ggtgtaggtc tggcattctt attggaatta ctcgatcgga
     2701 cagtgaaaga tgaaaaatat gtgacagata cattaggatt cccaatctta ggaacagttc
     2761 cagaaatgtc agcgaaagaa ttgaatgctg gtatccgtaa aaaaggacca gctcagacac
     2821 aaccaaaagc tacaaacgat aaaacagcgc catcacgcag aagccgctca agagtctagg
     2881 aggaaagaaa atggcacgaa cacagaaaca aaaagcagcc caaactcatg cagtcagttt
     2941 ggtcacactc accaatccct cttcaccaat cgctgaacaa tatcgtacga ttcggacgaa
     3001 tatccagttt gcctccgcag ctggtcagca aatcaaaacg attgttgtga cttcatcagg
     3061 acctggagaa gggaaatcga ccactgcagc aaacattgcc gttgtttttg ccaaatctgg
     3121 tcaacgggtg ctgctagtcg atgcagactt acgtaagccc gtcatctata aaacgttcaa
     3181 gttgaacaac gcaagcggat taagtactgt tcttagttca tcaggaagtg tagcagatga
     3241 gatccaacga acaccagtcg acaatttgtc gattctgcca agtggaccta aaccaccaaa
     3301 tccatcagaa ctactaagtt cgccaagaat ggatcagatt ttagcagaag caagacaact
     3361 atttgatgta gtgatcttcg acatgccgcc agtcgttgcc gtaacagatg cacagatcat
     3421 gtcttcaaaa acggatggca ccttattggt cgtacgggaa aatacgtcac gaaaagaatc
     3481 attgactaaa gcaaaagaac tgttagatat ggtacaagca cgagtcttag gtgtcgtata
     3541 caacggagca gaacacagca aagatgctgg ttattactac ggaaattaag gaataagaag
     3601 agaggagaca agagaaatga tcgatctaca ttgccatatc ctaccaggaa tcgatgacgg
     3661 ggcagaaaac ttagaagcaa gtattgcaat ggctgaaaaa gcgattcagc aaggcattac
     3721 gcatatttta tgcacaccac accacaataa tggaaaatac agcaatgaaa aatcacaagt
     3781 catttctcta gtcgcttcgt tacaagccga attagagaaa agacagttgc ctttgacgtt
     3841 attggaagga caagaagtac gtattaccgg aacgctcatc gaagacatcc accgagatga
     3901 aattttattt acagatttag atgatacata tcttctgatc gaatttccga cactggaagt
     3961 gccgttatat gcagaacggc ttttttttga gttgttagaa ctaggaaaga taccagtgat
     4021 cgtccatcca gaacgcaacg cgcattttcg agaagaccca aatcatttgc ttccgttcct
     4081 agatatggga tgtttggccc aacttactgc gccaagttat gttggtgtat ttggcaaaga
     4141 tatccagaaa acagccaaaa caatggtcaa acataatctc gtgcagatgg ctgcttctga
     4201 tgcacacggg gtctctaagc gcacctttta cttaaaagag tgctatgagc agattgccaa
     4261 ggagtttggt aaggaaaaag tagtacagat gaaacaagtc gcaaaggatc tgatcaacgg
     4321 ggatgagatc aattatccaa tctatgaaga ggtaaaaaag aagaagtttg gtttatttta
     4381 gttctttatt cgtagataaa aaggaaatga ggagagagtg agagatgtat agaaaaaact
     4441 caagtggttg gtctaaacat attgatttta ttttattaga cttattttgt gtccagcttg
     4501 ctttttatat atcatatgta atgcgacagg gtgactggaa tccgtatgtg attcctattt
     4561 atcgccacat ggcaattttt atagaacttg agaatcttac gattatattt ttatttgagg
     4621 catataaaaa tgttttaaaa agaggatatt ataaagaggc tgtagcttct ataaagcaat
     4681 cttttatgct gttacttgtg tgcagtttat accttattac aatgcaggat ggaaatgatt
     4741 attccagagt ttctttattt ataatggggg tggtgtatgg attattaaca tatataattc
     4801 gtatactttg gaaagggttg ttgcatcacc gcatgaaaaa cggtgaagat gtatcgttag
     4861 ttattgtaac atctcaaaag attgcaaaaa atgtagttcg taatcttaaa gagaagaatt
     4921 atggcatgta taaacttagt ggtgtgattg tagtagacag agatatgtat ggaactacca
     4981 tcggaggtgt cccagtagtt gcaaatgctg ataatgcagc ggaatatctt ttacagaatt
     5041 gggtagatga agtattcatc aatgttgacg aaagtgttcc atatcccaaa gaattaatcg
     5101 ccagatgttc ggaaatggga cttgctgtac atatgaatct tacaaaagtg tctgatagta
     5161 cacgtggaaa acaatttatt ggccgagttg gtgattatac tgttattaca tctagtatta
     5221 atgttatgac gatgaggcaa gcatttataa agagaacgat tgacattctt gctggaatta
     5281 ttggatgtat tgcaacaggt gttattttta tcttcgtagc accagttatt tacatcaaat
     5341 caccagggcc gattttcttt gcacaagaac gtattggtca aaatggtaag acatttaaga
     5401 tgtataagtt ccgtagtatg tatatggatg cagaagaacg caaagcagag ttgatgaaag
     5461 aaaataagat gagtaataat ctcatgttta agatggattt tgacccaaga atcattggca
     5521 ataaaatact tcctgatgga acaaagaaga caggaattgg acaatttatt cgatctacat
     5581 cattggatga gtttccacaa ttttataatg tcctaatggg aaaaatgagc ctggtggggt
     5641 ttagaccttg cttgaagtca gaatatgaga agtatgattt tcatcatcgt gcgagaattg
     5701 cgatgaaacc tgggattact ggaatgtggc aggtgagtgg tagaagtgat atcacagact
     5761 tcgaagaggt tgtaaaactt gatgcagaat atatcaggaa ttggagtatg ggattagatt
     5821 ttagaatttt gtttaggaca gtcaaaactg tgcttcatag aaacggttcc atgtaagata
     5881 aagatactat agaaggagta aaacgtgatg gcaagaaaaa aattaaaaat ctgtatgcta
     5941 ggtcataaaa gaattccatc aagagaaggt ggaattgaga tagtggtaga agaattggca
     6001 acgcgaatgg ttgcacttgg gcatgaagtg acttgctata atcgaagtgg tcatcatgtt
     6061 agcggaaagg aatttgacca gcataaaaat aaagaatata aaggtgtaaa attaaagact
     6121 atttttacat taaatataaa aggaattgca gcaatgagtt cttcagtatt tggtggaatt
     6181 agagcagcat tcggaaaata tgatgttgtt cattttcatg cagaaggacc atgtgctatg
     6241 ttgtggttgc caaaactttt tgggaaaaga tgtgttgcaa caattcatgg tctagatcat
     6301 caaagagaaa aatggaacaa gcttgctagt acatacatca tgttagggga aaagtgtgct
     6361 gtaaaatttg cagatgaaat aattgtactt agtgaaagcg tccaaaatta ttttgaagat
     6421 atatatggaa gaaaaacaag gtttatacca aatggcgtta aaaaaattga aataaaatca
     6481 gcaggattga ttacagaaaa gtatggatta acaaaagatt cttatatttt gtttttgggg
     6541 cgattggttc ccgaaaaagg aattcggtat ctaattgagg catttaaaga tgttcagaca
     6601 gataaaaaac ttataatagc cggaggaagc tctgatacag atgaatttgc aaatgaacta
     6661 aaggaattag caaaaggtga tgagcgaatt atatttactg gatttgtaca aggacaggaa
     6721 ttagaagaat tatatagtaa tgcgtatatc tatacacttc caagtgattt agaaggaatg
     6781 ccattaagtt tgttagaagc aatgagttat gggaactgtt gtatagtttc aaatatctca
     6841 gagtgtacgg aagtagtaga agacaaggct atgattttta agaaatcgga tgtaagtgat
     6901 ttaaagataa gattagaaga ggcatgcaat caatcggaaa tggttaaagt tttgaagaat
     6961 caagctactg agtttatttg tagtaaatat aattgggata aaatcgtaca agaaacatta
     7021 aatttgtata ggggaaatat ataatgattc gaatattaca gtgtgtgaat gatatgcatc
     7081 gtgcagggtt ggagacgatg ctaatgaact attataggaa tatagatcga actaaaattc
     7141 agtttgattt tttgacacat cgtccatata aaagtgatta tgatgaggaa ataataagtc
     7201 ttggaggaaa ggtctattat gcaccgagac tttatccaca aaattatcct aaatatttta
     7261 aatggatgga acaatttttt aaggatcatc cagaatatga gattgttcat tctcatattg
     7321 atgcgatgag ttatttacct ttaaaagctg caaaaaaagc tgggattccg atacggatag
     7381 cacatagcca taatacatcg atagatagag attttaaata tcttttaaag caatattatc
     7441 gatgtaagtt aacgtctgtt ttgacacatg aattttcatg tggaaaagta gcaggtgatt
     7501 ttttgtttcg aaatgataaa ttcactttga ttccaaatgc agtggatgct aaaaaatttt
     7561 attatgatga aagtattaga aatgatgtac gaatgaaatt aggaatttca agtcagacgt
     7621 ttgtaatggg acatattgga agaatttcat attctaaaaa tcatcgtttt ttgattgaaa
     7681 tttttaaaga atttcacgct attaagaata attccgtatt attaattatt ggaactggtg
     7741 atatggaaga agaaataaaa aattatgcaa agaaaagtgg catagatgat gatattaaat
     7801 ttttaggaaa tagaaatgat atagaaaaat tttatcaagc atttgacgta ttaatgttgc
     7861 catcattatt tgaaggggtt ccgttagtgg gtattgaagc tcaatttgca gatttaccgt
     7921 gtttcttttc ggagaaagtt cctacagaag ttgctttcag tgataagacc aatttcataa
     7981 gtttaaatca atcggcaaaa gaatgggcga gagagattgc aaatgtagat atctctcatc
     8041 gagattcaga acgttcgatt ttaataaagg cagattataa tattcaaaca gcatacaaaa
     8101 tcttagaatc aaaatattat gagttatttg aattaataca aagaggttaa agaatatgac
     8161 acagaaacat tcaagtataa aaagaattat acatcgcata tccgaagaat tgaatgcaac
     8221 aagacgaatt gcaaagataa catcttggaa ggaagcattt gccacgttac aagctaaaat
     8281 tgatattcaa gtcatgaatc gtaatggata taatgaaagt aaaaaacaga aggaacattt
     8341 attaaaaaaa catgaggtaa tgctgaagta ttatgaaaag actttttcag attttttgaa
     8401 aacatatgat tatagtcaaa atcaaataat taatcaatca aaatcaccat attctaactg
     8461 catttgggtt tgttggtggc agggattgga tcaatcgccg gaaattgtaa aagcttgtgt
     8521 aaaatcaata aaacgaaatg cagggaaaca tactgttata attttaacag aagataatta
     8581 tagacaatat gttgatatac caaaatgggt ggaagaaaag aaaaacaaag gaatcattac
     8641 gagaacaaat tattctgatt tattaagatt atctttgcta gcaaaacatg gaggtttgtg
     8701 gttagacgcc acattttatt gtacaaaacc aataccagat gagtattttg atatcgatat
     8761 ttggtctatt aggaggcctg attatgctca cgcatccgta gcatcaggat attttgcggg
     8821 ttattcatta gcatgtaatc aagagtctag atggatgttt gctataatta gagacttttt
     8881 tttaaattat tggaaaaata atgatatgat ggtggattat ttgatgatag attatatgat
     8941 tgttttagca cagagaataa atcatgaaat agcacagaca tttgataaaa tacctagtaa
     9001 caatccatta tgtgacgaat tgattaaaaa tttaaataat atatatgatg aaaaagtatg
     9061 gaacttatta aagaaagata ctttactgtt taagttgaca tggaaacaat cttttaaaaa
     9121 gcaaaaagat gataaagtta ctttttatgg gatgttggta gaaaacaaat tattataaat
     9181 attagaggta taaaaagtta tgaaacacaa ggtaagtgtg atagtaccaa tttacaatgt
     9241 tgaaaaatat ttaagaaaat gtgtggatag tttattgagt cagacattaa aagagataga
     9301 aatcgtatta gttgatgatg gttcgccaga tgcttcgggg gaaatagcag atgagtatca
     9361 aaaaaaatat tctaatgtaa aaacaataca tagagaaaat ggggggttag gaccggcaag
     9421 aaatacagga attgagaatg cgacaggaga atatgtggct tttcttgatt cagatgattg
     9481 ggtagaaagt gatatgtatg aaaaattata cttagttgcg tccaaagaaa atgctgatat
     9541 agtagttagt ggacattgtg attttgcgaa tgacaaagct atggtgaaaa agcaacatcc
     9601 tttgagtggg aatgtatatg atacgcctaa tagtattaaa aatgtacgaa aaaatttatt
     9661 tggacatagt ccaaaagatc aagaagtaga ggccttcccg atgtcagtat gtatgtcttt
     9721 gtataataaa aatctgctag ataaatatag actaagattt gaaaaaattt tatctgaaga
     9781 tacaattttt aacttgaatg catatgatgt cgcaaataaa attgctttta cagaatatac
     9841 agattattgt tatagaaaag aagaacaaga gtctattaca aaaagtttat ctccaaataa
     9901 gcagacggaa tttaaagaat ttttaataac tctaaaagct atagcatcga aagaagattt
     9961 agaatgtcaa atcagagcaa aacgtatggc aattgattat tgtaggcttt atgtgggctt
    10021 agttgatgat gcaaatttgg gaattaaaga gaaaagaaag tatataaaaa aatttattga
    10081 agatgaggga ttaattcaat gttggaaggg atatcctaca aatacgctac catttcaaca
    10141 aaaatttttt cataaaatgc ttgaatgtaa gtgttataca atattgttgt tgttaagtag
    10201 aattagaaag ataattaaaa atatgaccag aaaaaaattg tttataatat tataaactaa
    10261 aagtatatca aataatagaa attaacaagt tacaaaaaaa tattataaag aataatattt
    10321 tctaaaaagt aaactatagt aatgtggatt atcaccttcc aacagtatat aaaatgagcg
    10381 taaaaacggc tatttttgcc ttaacaacga tttttttgta tacaaatacg atatgctcat
    10441 taattattgg aatttgtagt gctgtttaaa ttaatgaagg tgtaattata tgagtgatca
    10501 ttttgaaaaa gtttgttgag aagatgtgct tctagtcatg tgatgggaga aaataattta
    10561 actgttagag tgaatagtta aattattctg gttatatgaa aaaagtagtt tctaaaataa
    10621 tcttgcaggg attaacttaa agaaattgag agaatagttt ctttgaaatt tctatataca
    10681 atgagaagct gaaatttact tttatttgat gactggaaat agaaattaaa tatattgtat
    10741 tgttacatta aagaaaactt gtatggaaaa atcagtacaa taccattatt taatgttctt
    10801 cacttaaagc gcaaaaaata tcagtttatt aaactcgtat atagaacaaa tatatgtaga
    10861 cggggcgaaa cgatgagtgt aaaaaaaatg ctatttatgg taagtacaat aattgtatta
    10921 acatcccgat tttggggaat taatactagt ttatctatgc gatattttat tatggcaata
    10981 tgggatttat attttatgtt aagctatttt tcttatcgtg agaaaacata taaaaaatac
    11041 gttatcaaga ataacttttg gttgactata atgccgtttg tgatattttc tatctatact
    11101 ctaataatat ggggaattgg ttcaaatgca gaatttgaaa attttataaa gttatgttcc
    11161 acgttgcttt atttaattct tgcatatggt tacgcatgta cagcatttta cttatttaag
    11221 gcagaaggaa ttgattttat tttttggtca ggtgttattt catatatgag tggttcagtt
    11281 ttttatttaa ttgtatctta tggtttaaat ggtttaattt cttatataaa aagtcttata
    11341 acaggtgaaa caaatactgc aaattttgct atggaggtac atgatttaac ttttgcgatg
    11401 ggggtttttt tcctttatta cgtatttttt gaaaataaaa atacaaaaaa acataagata
    11461 aaaattatcg tatctttaat tttaattttc atgggattga agagaattga actgttagct
    11521 ttaagtggtg caattctagt gtattatgtt ttattaaagt ggggaaaaaa gattcaaatc
    11581 cgtgcattag tgtgtactat ttgtgcaact gttatttcgt tagtgtttat ttacattata
    11641 gatacaggaa ttttggcatt gcttatgtca aatttgggca ttacagatga cggtgcgagg
    11701 ctttcatttt atctatattc atctaaattt tatgaacttg gtttaggata tgtaggaaca
    11761 ggatggacat ggttttcaaa atactttttt aatctttatt tatcgcactt taggttaaat
    11821 ggtggaagaa ttgcagcaag cctacacagt gatatattaa ctatttatat agaagttgga
    11881 tgtatagttt ttattatatg gatgttttgg ttattttttg tgaaaccgat aagactaact
    11941 aaaaaatttg gattgaaaac aggggagtgt gtattattat taactatata tatgtttata
    12001 ttatatttga cggataatac gctcacttat ccagacactc aaatgatttt tctcttagta
    12061 cctatgatag tttcgtgggt tgataatcaa caacgaatat cggaatagat agctaaagta
    12121 tattttaatt atgaatttat aattagttgt aaaagttaat atttaacgga caggaagtag
    12181 ggtgaaaaaa tttgaaaaaa attttagtgt tagcaaatac atattatcaa ataatagtag
    12241 caatacaaat gaaaaataca attttttccg atgagtatgt tgaaattatt atttctgatc
    12301 attctaaaaa cactaaagaa attgcgaaaa aaatagaaga aaaaaaagtg tttgatggct
    12361 gtcattattt agaaagcaaa tcttttgatg ataatattgg gtattttcaa aagattaagg
    12421 actgtcttca gatttcttgt ggtaaatcaa atcgatatgc ctattatttg aaaaactgta
    12481 aaagtgaata ttttgatgaa tttatttgtt tttcaatggg agaggtttgg ttggaaggaa
    12541 tatatgcttg cttatatcga aataataaaa atgtacaggt atcaatttta gaagaaggta
    12601 ttttaagcta tggagttgac attattccaa cagcaagaac aaaagctatc gcagtactca
    12661 attacttaaa aaaaaggact ccactatata acaaaagcta taatttttat tgtttttttc
    12721 ctaatatata tacaggaaga gagaatacaa tacaaattcc attagttaca gctgggtcaa
    12781 gaaccgcatt gcaactttcg gatattttta accctatgat agatatttat aaagaaaaaa
    12841 aatatatatt ttttacgtct gtttatgatt ttgagggcgg agaacctgtc ggagaatatg
    12901 aattagtatg caaaatagca gacttagtag gaaaagataa tctaattgtt aagacgcatc
    12961 ctagagatgt aagaacaatt tataaagata atggatttat cgaggataaa aattcatcaa
    13021 taccttggga ggcgattcaa ttgtcaggcg attttaacga taaaatattt atgacaatta
    13081 attcaggaag tgttatttca ggtagtacaa tgtctgcaaa acctgtacgc acgtattata
    13141 tgtataaatt gtgtaacata gaaggaaatc cgtcttgcaa aaagaatgcg tatgatattg
    13201 agcgattgct taagcaaaaa acgatgaaaa acattttaaa gaatgtgaaa atagcagaaa
    13261 gcttagatga tatactatga gcaaaactaa atatttaatt aaaaatatgg gaatattaac
    13321 aataagtaat ttttcttcaa aaattttagt ttttttgtta gtccctttat atacaagtgt
    13381 attatccaca acagaatatg gcacatatga tttgacaatt tcaacagcca cattattgtt
    13441 tccaatttta accttaaaca ttgtagatgc tgtgatgcgc tttttaatgg ataaagataa
    13501 aaataaaaaa aatatagcat ctatagcgat taaagttata tttttaagcg tgatagtctt
    13561 tggatccgta atgtatttgt taaatattac aaaattatgg caagatgttt atggattaga
    13621 aaaatatatt tttttgtatt atatatccta tgtatggaat caatttctaa tacaatttgc
    13681 taaagggtta gaaaaagtta aagctatggg catagcaggt attatcagca caattgtaac
    13741 aataattgcc aatattttct tcttgcttat tctgaaatgg ggattaattg gttttttcat
    13801 tgcaaatatt ttatcacaga ttatttcagc tacttatctg tcagtattag tgagaatatg
    13861 ggaatatatc gattttgagg ctaaagatcc tcagctaaag agagaaatgc taaattactc
    13921 gattccatta attgcaactg ttgttggatg gtggattaat agtacagctg ataggtatgt
    13981 ggtttccttt atagtaggtg tctcggcgaa tggcttattg tctgtggcat ataaaatacc
    14041 tcaaataatt aatactttgc agggaatatt tatacaggcc tggcaaattt ctgcaattaa
    14101 agaatatgga gataatgaga ctgcaaaatt ttatggaaat acatttgtta tgattaattt
    14161 gttaatgtgc ataacctgtt caagtcttat catattaact agaccgtttt cacatattct
    14221 ttatgcgaaa gacttttatg aagcgtggaa atatgttcca tttttattaa tagctaatgt
    14281 tttaaattgt gcatcaggtc tgttaggacc tatcttatca gcaaaaaaag attctaaatc
    14341 catgatgtgg tctgcaatca taggggcatt agttaatgtg attatgagtg tagtccttgt
    14401 ttataagata ggggttcaag gtgccacaat agccactgtt atatgttcat atgtaatata
    14461 tgttgttcgt aaacgagctg tgggaaaaga tatttttata aagcattatc atattgttgt
    14521 ggaatcttgg ggattattgt ttgttcaggc aatcatagaa atatatttta aaaattatgt
    14581 aatagaaatt ataattatga ttattttaat tattataaat atcagttata ttaagcagtt
    14641 gtgttataga ggaagaaaca ttatttcaat gattaagagt cgagagcaca aacggtagac
    14701 gatgttattt ttataaaaag atataattta ttaatggtcg ataattatga aaggaaaaat
    14761 attcttaaaa aaaagagtat aggatgtcgt atgaaaatag tcgctatagt accaatgaag
    14821 ttaaataaca aaagattgcc acaaaaaaac acgaaagctt ttaccaatgg aaaaccattg
    14881 tgttattata tactttcaac acttttaaaa gtaaaacaga ttgataaagt gtatgtctat
    14941 tgtagtaacc cagatattaa agaatttata ccagaaggtg taacattttt aaagagatct
    15001 agttccttag atcaggatac aacgagtatt acacaggtat tagccggttt cacaaaagaa
    15061 gtagaggctg atgtatatgt aatgacacac acaacagcac catttattag cgaaaaatct
    15121 attgaaaaag gactagatgc cgtgctgaca ggagaatatg attctgcatt tgctgcaaaa
    15181 aaattgcaag atttcttatg gaaagacggt gaaccattta attatgaatt ggacaatatt
    15241 cctagaacac aggatttgcc gcctttatac gaagaaacta gtggatttta tatttataat
    15301 cgcaatgtga tgaccgagtt gcatagaaga attgggaaaa aaccttgcat agttgaagta
    15361 ggtgagattg agtcagttga tattgatgaa gccgaggatt ttatgattgc tgatgcgatt
    15421 tataatcatc ttattttaca aggtagttaa gacaggggga aggttaggat gaacaatgta
    15481 cagattttag attgcacact tagagatggg ggctattgta acaattggga atttggattt
    15541 gataatacaa aaaaaattat ttctggactt atagaatcag gaattgagat tatagaatgt
    15601 gggtttctca caaataaaac agtatacaat aaaaatgtta caaaatatac gaccttagaa
    15661 gaattagcac agataattcc tgaaaaaaga gaaggaaaat tatttgtagc aatgatgaac
    15721 tatggcgaat ataatataga ggatcttcca gattatgatg gttcatctat tgatggaatt
    15781 aggattgcgt ttcataaaaa aaattgcaat gaagcgttga aattatgtcg tgagataaag
    15841 aaaaaaggct acttagtttt tgttcaagct atggtatctc tcaattatac agatgaagag
    15901 tttttagcag taataaaaaa tgtgaatcag tttaaaccat acgcttttta tattgttgat
    15961 agttttggaa tgatgaaagg aaaagattta actcgtcttt tttacatgat tgagcataat
    16021 ctagaaaaag atatacagat aggttttcac tcacataata acatgcagct tgcatattct
    16081 aatgcacaaa aacttataac cgtacaaaca aatcgaagcc tgattattga ttcttcggtg
    16141 tacggaatgg gaagaggagc aggtaatctt aacacagaac tatttgtaga atatttgaat
    16201 gataatgcag gaaaaaaata tcaattaaaa ccattattat atataattga tgaaatacta
    16261 aatagtttct atcagaagag ttattgggga tattcattac caaactatat ttcagcagtt
    16321 cataatgctc atcctaatta tgcaagctat ctgtcatata agaatacttt aacaattgaa
    16381 gcaatgaatg aaatattcga aatgatggat gaagaaaaaa aggtatctta tgataaacaa
    16441 tatatagaag atttatacta caaatatatg gaaactggga aaatacagga ggaatatatg
    16501 gaagaactga gaaaatttct ctcagacaaa aaaattctac tgattgcacc aggaaaaagt
    16561 tctgtagatg aggtagaaag aataaaagag tttgttacca aagaagaagt cgtaataatt
    16621 agtgtaaatt ttgaatattc tcatatagat atagattacg tatttttaag taatttaaga
    16681 cgttttcatg aattaccgaa agaaagcaga actaaatgta ttgttacatc aaatatacaa
    16741 gcatcggatg tatatttacg gacgagttat aaaaagttgt tatctgaaag ggaagttgta
    16801 agagacaatg caggattaat ggcaattaga tttttagccg atatgaatgt ggatgaaata
    16861 ttcttagctg gatttgatgg atattcacat aatgaagatg agaattatgg tgagcaatcc
    16921 atggaaatta taacaaaatt tgcattatta gatgcaatga acgtgcaaat gagagatgaa
    16981 ttgtcagaat tagctaaaaa agtaaaggtg acatttttaa cggatccacg ttgggttcga
    17041 attgataagt agtagtaata atgtttttaa aattttgata aagagatagt gagagaataa
    17101 aaatgggtaa agtcttaaat aaaaaagaag aaaaatattt aagggacgat ggggcgagat
    17161 gaaactctaa ttttgaactt ttaagaatca tttctatatt gtgtattgtt gcttaccatg
    17221 ctaatttgcg tttaaattat aatgcagacg gaattatagt acatggttca ttaactgcta
    17281 accagatttt cttgaattat attggtattt ggggtcagct aggagttaat tgttttatat
    17341 ttatttcggc atatttttta agtgagaaaa gaaacattca ctttgaaaat gttttgggaa
    17401 ttgtattaga atgtattttt tatagtgtat tagtttatgg aattctttgt gcatgtggaa
    17461 tagaaacatt ttcattgaac agctttgtaa aagaaatatt atcatttgct ctaggtagat
    17521 attggtttat tacatcctat ttagcattgt atattctaag tccgtattta aatatcatgc
    17581 ttaacaatct ggaaaaactt cagttgcaaa aaataagtat gttattgttt ataggtgtat
    17641 gctgttatcg tatgttattt aaaacatctt tgtttgggga ttgtgctgtt ttaattgcac
    17701 tatatgtcat atgttataca ctgaaaaaaa cgataataat atttttgaga gacacagaat
    17761 cataggaaca attgtatgta taacagttat accacttctc atatccatag gaatactttt
    17821 caaatttata ccaaatgcaa tggttaacca gatggttcat attggttctc cattgatggt
    17881 tatagcaagt atatttttat tttatgtatt taaaaatttt tcattttaca gtaagtatat
    17941 aaataaactt gcgagaacca gtttaggggt atatatttta catgaaaatc attatttcat
    18001 tgatggtatg aagtctagag gcttaatatg ggatgggata tttggattag gcaaaaggtt
    18061 tttagatagg gatttttgga tttggtttat aattgcagtt gtagttgtgt gcataatttg
    18121 catggctatc gatgttgtaa gagaaaaatt tgaaagacag atttttaaaa ttaagagtgt
    18181 aaagaacctg tgtaaactta tgaatcatgt tataaatata tacccataat taataaatga
    18241 ggagaaaaga agtgaaagtt atatcattcg gagaattatt attgagactt gcagcacccg
    18301 gttattcaaa gttatttcaa agagatagtt tggagactac attctgcgga ggggaagcta
    18361 atgtagcggt atccttagca atatttggat atcaatcaga atttataaca aagcttccgg
    18421 attcagatgt tggatatgcg gcagctaatg ctttgcgtta ttttggagta aatgttgata
    18481 atattatatt tggagaggga cgcatgggac tgtattattt agaaaaagga gcagctcaga
    18541 gaccatctaa agtaatttat gacagaaaat tttctgcaat ttcgttagct gatagtgatg
    18601 aatttaagtg gaaggagatc ctaaacgatg caaattggtt tcattggaca gggataaatc
    18661 cagcattgtc tgagaatctc tttgagacgt ccttagatgc atgtcaggct gccaaaaaaa
    18721 gaggtataac aatttcttgt gatttaaatt atcgatccaa gctttggaca tcagaggaag
    18781 caaaagaaaa gatggaacag atactaccat tggttgatat ttgtatttgt aatgaagaag
    18841 atgcagaaaa agtgctagga attaaagctg agcatacaaa tattaatagt ggagaattaa
    18901 ataaagatgg ctatgtgcag gtggctaggg agattcacaa gaagtttgga tgtaggtatg
    18961 tagctacaac tctgcgtaga agttattcgg caagtagaaa tggttggagt gcaatgttat
    19021 acgaagcaga aaaagataaa gcgtattttt ccacagaata caacatacaa attgttgatc
    19081 gcgttggtgg tggagacagc ttttctgctg gtataatata tgcgttatgt gaaaatatgt
    19141 ccggacaaaa ggctattgaa tttgcgactg catctagttg tttaaaacat actatagaag
    19201 gtgattttaa tcgaactaca attgaggatg tatataattt aatgaatagc ggtggaaatg
    19261 gtcgcgtgtt gcgttagtca acatataaag ataaatcaca gtaattttta tggtgattat
    19321 ctgtcttagt agtgattaga ataacgtgaa aataaattgt tgagcgttta tactgttttg
    19381 aaaaataaat ttaacatatt gaaaaataca aatatatacg tatattataa agcgccgatt
    19441 gtaaaattaa gctagacaac taaaacagtc atttgtgcta cactcaaaat agttccgacc
    19501 aaagaattat aaggagtgag tgcaaataac ctatacctat cttacaacgg atgaacttgt
    19561 aataatagag tcttatttga aaattaacca atctgttgct aaaactgcgc attgcttgaa
    19621 tcgttcaagt caaacgatcc ataaagtata tctgttcttc aagcaaagaa aatcagcctt
    19681 agaata
//
LOCUS       DAFCNJ010000048        19501 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000048 DAFCNJ010000000
VERSION     DAFCNJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.049"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..3660
                     /locus_tag="LNE35_002082"
     CDS             133..3660
                     /locus_tag="LNE35_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBK6911206.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="LNE35_002083"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="LNE35_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBK6911207.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="LNE35_002084"
     CDS             7448..7789
                     /locus_tag="LNE35_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6911208.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="LNE35_002085"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="LNE35_002085"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBK6911209.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="LNE35_002086"
     CDS             9146..11566
                     /locus_tag="LNE35_002086"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBK6911210.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LNE35_002087"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LNE35_002087"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBK6911211.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="LNE35_002088"
     CDS             13000..13737
                     /locus_tag="LNE35_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6911212.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="LNE35_002089"
     CDS             13751..14566
                     /locus_tag="LNE35_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK6911213.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="LNE35_002090"
     CDS             14579..15226
                     /locus_tag="LNE35_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6911214.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="LNE35_002091"
     CDS             15242..15898
                     /locus_tag="LNE35_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6911215.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="LNE35_002092"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="LNE35_002092"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBK6911216.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="LNE35_002093"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="LNE35_002093"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBK6911217.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="LNE35_002094"
     CDS             18240..18758
                     /locus_tag="LNE35_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK6911218.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="LNE35_002095"
     CDS             18865..19365
                     /locus_tag="LNE35_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6911219.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6559 a         3347 c         4185 g         5410 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 t
//
LOCUS       DAFCNJ010000049        19474 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000049 DAFCNJ010000000
VERSION     DAFCNJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.050"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(22..996)
                     /locus_tag="LNE35_002096"
     CDS             complement(22..996)
                     /locus_tag="LNE35_002096"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK6911220.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="LNE35_002097"
     CDS             complement(1076..1951)
                     /locus_tag="LNE35_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBK6911221.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="LNE35_002098"
     CDS             2081..3988
                     /locus_tag="LNE35_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBK6911222.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="LNE35_002099"
     CDS             complement(4529..7807)
                     /locus_tag="LNE35_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBK6911223.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8078..8623)
                     /locus_tag="LNE35_002100"
     CDS             complement(8078..8623)
                     /locus_tag="LNE35_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6911224.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8635..9327)
                     /locus_tag="LNE35_002101"
     CDS             complement(8635..9327)
                     /locus_tag="LNE35_002101"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBK6911225.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9346..9630)
                     /locus_tag="LNE35_002102"
     CDS             complement(9346..9630)
                     /locus_tag="LNE35_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911226.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9643..10206)
                     /locus_tag="LNE35_002103"
     CDS             complement(9643..10206)
                     /locus_tag="LNE35_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK6911227.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10434..11096
                     /locus_tag="LNE35_002104"
     CDS             10434..11096
                     /locus_tag="LNE35_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBK6911228.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11220..12416
                     /locus_tag="LNE35_002105"
     CDS             11220..12416
                     /locus_tag="LNE35_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBK6911229.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12517..13893)
                     /locus_tag="LNE35_002106"
     CDS             complement(12517..13893)
                     /locus_tag="LNE35_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6911230.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13930..14796)
                     /locus_tag="LNE35_002107"
     CDS             complement(13930..14796)
                     /locus_tag="LNE35_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBK6911231.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14885..15910)
                     /locus_tag="LNE35_002108"
     CDS             complement(14885..15910)
                     /locus_tag="LNE35_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6911232.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15928..16602)
                     /locus_tag="LNE35_002109"
     CDS             complement(15928..16602)
                     /locus_tag="LNE35_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6911233.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16786..17679)
                     /locus_tag="LNE35_002110"
     CDS             complement(16786..17679)
                     /locus_tag="LNE35_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6911234.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17753..19450)
                     /locus_tag="LNE35_002111"
     CDS             complement(17753..19450)
                     /locus_tag="LNE35_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6911235.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5683 a         4189 c         3446 g         6156 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtcaataaa aaacaaaatt ataataagtg tattttataa aaagaaaaaa
     7921 tataacttaa gttgttgata ataaagagat ttattaacgt aaaaagaata agttttttta
     7981 aaataacgta taaaaaagag cataaaacaa agtcgtttgt tttatgctct tcttttatta
     8041 ttttgtgctt tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg
     8101 tggactcaaa atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggcat
     8161 ctttgaaaat atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac
     8221 agatatctgt acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga
     8281 tgtctagatc tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt
     8341 cctcttgctc atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg
     8401 ttcctatcac attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat
     8461 catgcgcatc aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag
     8521 cagtcagctt tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg
     8581 caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac
     8641 gagatgtttg acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa
     8701 ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa
     8761 aaatgggata ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc
     8821 taatgcttca gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt
     8881 atcacctgta acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac
     8941 catttcactg cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc
     9001 tggtttgtag ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac
     9061 gatgtcacct acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat
     9121 gttcacgccg tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat
     9181 accggaacgt acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc
     9241 tgcgcgttcc catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc
     9301 cattgcgccg ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat
     9361 tgctaaaaaa accacacata acattaggat aacaataata agagatttca tcaaaaaaga
     9421 gttccatttg gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt
     9481 tttgctttct ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc
     9541 tgcttttcgc tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc
     9601 gcttcgcgta ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa
     9661 tagttcccag tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat
     9721 cgcttgttgc gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg
     9781 tgctaaagga ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt
     9841 tgcaaaacct ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc
     9901 agtttcttct tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc
     9961 gatttttcca gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgtttgac
    10021 aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg acaccaccag gatgaaatac
    10081 cagttctctc ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat
    10141 aatttttccg ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt
    10201 cagcatatct tctcctgctt tctttgtaat cataactgct tttttttcat ttttcaattt
    10261 ttctcaaaaa ctccttatta gtttaccata gataaacctt agattttctc taaatataga
    10321 aaatccttct ttagtccgat actctttcta gtagtccgta tgctaaaata agtatgtaat
    10381 cgaataacga aaagaattca atactcaacg ataaatgaca tggagggttt attatgataa
    10441 aacgaaattg gccgtggctt ctagcattgg tcttgctatt tctctttgtc cgaaacaata
    10501 gtttttcttt tgttctagga cttttattga taggtgtagt tttagttttg tggggactct
    10561 ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc tgcactgact gacgaacttg
    10621 aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa
    10681 tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac
    10741 ttaaagcaat cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat
    10801 tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga
    10861 atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac
    10921 agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg
    10981 cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg
    11041 ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct
    11101 tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt
    11161 tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta
    11221 tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt
    11281 tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga
    11341 ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac
    11401 gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca
    11461 tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc
    11521 atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc
    11581 tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct
    11641 tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac
    11701 aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc
    11761 tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata
    11821 tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga
    11881 aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat
    11941 tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc
    12001 agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc
    12061 aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct
    12121 tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact
    12181 tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg
    12241 aacgtggaat cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa
    12301 tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag
    12361 agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca
    12421 cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg
    12481 tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg
    12541 tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat
    12601 ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg agacgattgg
    12661 caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct
    12721 caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag
    12781 ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc
    12841 ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag
    12901 taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt
    12961 taccgtcagt agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc
    13021 tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat
    13081 tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat
    13141 gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc
    13201 atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc
    13261 aaaaggcatg aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa
    13321 caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt
    13381 ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat
    13441 gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa
    13501 acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag
    13561 taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac
    13621 atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact
    13681 tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaagtgaca gtgctggacc
    13741 agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc
    13801 aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt
    13861 gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga
    13921 aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag
    13981 gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat
    14041 cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc
    14101 aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat
    14161 catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc
    14221 taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt
    14281 gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt
    14341 accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt
    14401 caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt
    14461 ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc
    14521 agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt
    14581 ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat
    14641 tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc
    14701 tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca
    14761 tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat
    14821 gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga
    14881 ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc
    14941 cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg
    15001 ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa
    15061 atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa
    15121 ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg
    15181 gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat
    15241 cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat
    15301 aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga
    15361 ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat
    15421 cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga
    15481 tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt
    15541 caatataatc tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt
    15601 cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac
    15661 tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc
    15721 tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta
    15781 aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg
    15841 tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa
    15901 aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt
    15961 ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac
    16021 tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc
    16081 ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt
    16141 gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat
    16201 gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa
    16261 tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc
    16321 ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat
    16381 cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag
    16441 attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt
    16501 gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg
    16561 tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt
    16621 tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa
    16681 tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct
    16741 tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt
    16801 ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac
    16861 tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc
    16921 tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg
    16981 agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat
    17041 tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag
    17101 catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt
    17161 aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc
    17221 tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt
    17281 cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat
    17341 atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg
    17401 atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt
    17461 ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg
    17521 ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata
    17581 tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta ataaaagact
    17641 gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa
    17701 aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt
    17761 cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga
    17821 ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat
    17881 tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc
    17941 ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt
    18001 ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt
    18061 atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat
    18121 ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag
    18181 aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat
    18241 catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt
    18301 cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat
    18361 ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg
    18421 catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg
    18481 tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg
    18541 tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat
    18601 ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat
    18661 aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag
    18721 aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct
    18781 ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg
    18841 tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca
    18901 tcactggcat cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag
    18961 tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg
    19021 tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat
    19081 attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat
    19141 taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt
    19201 ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag
    19261 aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg
    19321 taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttggctagt tttccgttcc
    19381 cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt
    19441 ttttattcat ttttttctcc ccgttctttt ttta
//
LOCUS       DAFCNJ010000050        19199 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000050 DAFCNJ010000000
VERSION     DAFCNJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.051"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            101..2542
                     /locus_tag="LNE35_002112"
     CDS             101..2542
                     /locus_tag="LNE35_002112"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBK6911236.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="LNE35_002113"
     CDS             complement(2872..3576)
                     /locus_tag="LNE35_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911237.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="LNE35_002114"
     CDS             complement(3763..4008)
                     /locus_tag="LNE35_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911238.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="LNE35_002115"
     CDS             complement(4025..4876)
                     /locus_tag="LNE35_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6911239.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="LNE35_002116"
     CDS             5042..6046
                     /locus_tag="LNE35_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBK6911240.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6287..6643)
                     /locus_tag="LNE35_002117"
     CDS             complement(6287..6643)
                     /locus_tag="LNE35_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6911241.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7011..8198
                     /gene="tuf"
                     /locus_tag="LNE35_002118"
     CDS             7011..8198
                     /gene="tuf"
                     /locus_tag="LNE35_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK6911242.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="LNE35_002119"
     CDS             complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="LNE35_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK6911243.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11003..11764)
                     /locus_tag="LNE35_002120"
     CDS             complement(11003..11764)
                     /locus_tag="LNE35_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6911244.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12042..12563
                     /locus_tag="LNE35_002121"
     CDS             12042..12563
                     /locus_tag="LNE35_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBK6911245.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12622..13482
                     /locus_tag="LNE35_002122"
     CDS             12622..13482
                     /locus_tag="LNE35_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBK6911246.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13667..15982
                     /locus_tag="LNE35_002123"
     CDS             13667..15982
                     /locus_tag="LNE35_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6911247.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16164..18005
                     /locus_tag="LNE35_002124"
     CDS             16164..18005
                     /locus_tag="LNE35_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBK6911248.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18029..19138
                     /gene="rpoD"
                     /locus_tag="LNE35_002125"
     CDS             18029..19138
                     /gene="rpoD"
                     /locus_tag="LNE35_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBK6911249.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6226 a         3392 c         4028 g         5553 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc
     6181 taaacacgta taaacggcgg aagcagaagc aactccttct tatttcttgg agttaaacgc
     6241 ttctgtccca gcctcttttt atctaataaa attttaagac catttactaa ttatcgattt
     6301 tttctgccgc atttttaggc acttcagaag cctttatttc ttcccagctt aatacacctt
     6361 tacgaggatt gactttgagt ttaagatacg cattcatctt taatggatgt tctctttctt
     6421 cgcgaagcgt cacctgtttt ttttcaccat ctttattata agccgcttgc ttgtaggtat
     6481 aagtcgtgaa tttctctccg ttatctgctt gggtagctga catttcgcca ttcgtcgtga
     6541 tttttgtata ataagcttcg ccgccgtaaa aataatgata agctgcaaaa cttccgccga
     6601 gaaatatgcc taaaacgaca agtacgccaa ttaatttttt catgtacttc acctctttcc
     6661 ttattattaa cgaaatagaa gatttcgtct attgaaagtt gttggcaaca gctgaatttt
     6721 aaacttttcc tcattttgtt cttattgctt caaaagaaag ttaaggacta tcttgaagtt
     6781 tttctcaaaa aacacatttt tttatttata aaaggtactt ttttcgcgta taagacagac
     6841 gagtgctctt agttgacttt gaccaatatt gacgtataat aagcttagtt cgaatcttac
     6901 aaaaagtaag tcggacgtat tctttttgat ttagtcaaaa aaagactata ataaaaaggt
     6961 gagagctgaa atttttcagt gatctatatt atttaggagg tttgtaaatt atggcaaaag
     7021 aacattatga cagaagtaaa ccacacgtta acattggtac gattggacac gttgaccatg
     7081 ggaaaactac tttaactgct gcgatcacta cagtattagg caaaaaaggc ctagcaaatc
     7141 ctcaagacta tgctagtatt gatgcagctc cagaagaacg tgaacgtggg attacgatca
     7201 ataccgcaca cgtagaatat gaaacagaaa aacgccacta cgcgcatatt gatgcaccag
     7261 gacacgcgga ctatgtgaaa aacatgatta caggggcagc acaaatggat ggtgcaatct
     7321 tagttgtttc tgcaactgac ggtccgatgc ctcaaacacg tgaacacatt ttattgtcac
     7381 gccaagttgg tgtaaaatac ctgattgttt tcttgaacaa agttgattta gtcgatgatg
     7441 aagaattgat cgatttggta gaaatggaag ttcgcgagtt attgagcgaa tatggtttcc
     7501 caggcgatga cactcctgtg atcaaaggtt ccgcattaaa agcattgcaa ggcgatccag
     7561 atgctgaagc tgctattatg gaattgatgg atacagtaga tgaatatatc ccaacaccag
     7621 aacgtgatac agataaacca ttactattgc cagtggaaga cgtcttctca attacaggtc
     7681 gaggaactgt tgcctcaggt cgtattgatc gtggtgctgt tcgtgtcggt gatgaggtag
     7741 agatcgtagg gatcaaacct gaaacacaaa aagcagttgt aacaggtgta gaaatgttcc
     7801 gtaaaacgtt agattacggg gaagctgggg ataacgtagg cgtgttgtta cgggggatcc
     7861 aacgtgacga tatcgaacgt ggacaagtac ttgctaaacc aggttccatt actccacata
     7921 caaaattcaa agcagaagtg tacgtgttga caaaagaaga aggtggacgt catactccat
     7981 tcttcaacaa ctatcgtcca cagttctact tccgcacaac tgatgttaca ggaacaatca
     8041 cattgccaga agatacagaa atggtcatgc ctggtgacaa cgtgacgatt gatgttgact
     8101 tgatccatcc aatcgctgtt gaaaacggaa cgacattctc aattcgtgaa ggcggacgga
     8161 cagtaggttc aggtatcgtt acagagatcg aagcataaga aacgcgactc gaatattttg
     8221 gtaatgaaaa atgtttaaaa taaaagagcg gtccaaacag gatttctgtt tggaccgctc
     8281 tttgttcttc aattgtataa gaagagattg tagaagcatg aactttcgtt ttacttcagt
     8341 ctcttttatt catcatgaga gtgttcatct atcggtatgc agtttttaat actttcttcg
     8401 tatccatttt ttcaaaccac ttattctacc ttgtgtctgc tcaactggcg cattatcaat
     8461 aatcagcaaa gcaatcataa catatgcaat ccaactatct ctagaatata gagtataacg
     8521 tgagacccag tttgtagcgt acttgctttt atattctctt aatccatgga aagaataaaa
     8581 atgagaacca aactcgtaaa ccaagtatgc aatcctttcc tgtaaaaaac tctttctgga
     8641 agttccgaca tttgaaagag gggccatccc taaattgaac cattggatat gttcttcttt
     8701 catataggca aacaaatgca agaacaagaa atccatactg ccagaaggtg cttttttaga
     8761 atcatatcgc atcagatcaa tcgttcctac tttattgttc gtgtaagtag gcatgatcgt
     8821 tgcaaaagcg acaatttctt cttgggtatt tttgacaact gcaataggcg ctctttgtag
     8881 ataatcttca gagaaaaagc ctaaagaaaa ccctttttcc tttcgtgaac ccaaccaatt
     8941 atcagagata tttttgaaag tactcatttg ctcagtagaa aatggtggtt gtaagacatc
     9001 aaatgtaaat ccttctcgtt cgatccgatt caagacagca cgagtgccct tcattttttt
     9061 ccctgaagtt gtaaaactat tcaggtctac ataagcttct tcccccatct taatgaaatc
     9121 gtagccgaat tcatgcaaaa tcataacaat ctcttcgctt gtttcgtaga aaactggtaa
     9181 atagcatagt cgatctgttt cttcgataaa agcttcgatt gcttctggaa aatcttcttt
     9241 cttaccagaa ggatcaccca ttacaataca tttattattg aagcaagcga attggagaag
     9301 aacagttggt tcaccgtctt tttcataggc aaacatccgc ttatctttta agaagatcaa
     9361 ttgactatcg gagtttcctc cataagttgt aaggatcttc agggcctttt cttcattaaa
     9421 ggcttcgcca atctgtttct tttctccttg aaggaaatga acaaatagaa cgatcatgaa
     9481 actaacagcg atgatggcca acaatcccga aaaccaaatc ttttcagaag gaaataagaa
     9541 aaaggaaata aaatggtgac gccgatgagg gaaatccggc agattgtata ccccaataac
     9601 gatgtaaagc aaggacagtg caccaataat gattccatcg atcgtacgcc actcccatga
     9661 atagatcaat tgttcccgaa ataattcgtt tttagaagca agaatgatca gaagcagaat
     9721 agacaagaaa accaccggtg tgaaactaaa atcacttaat aaaacataaa gtaaagctag
     9781 aacaatcaag aagattgtag gcaaatatgc tcgtttcaca cgtgctgcga tccctctgcc
     9841 cataatgatt aaaagaaatc ccaatagtat actaggaaat tggataataa aatgaaattt
     9901 caatgggttc aaactatgaa gccagcgaaa ctctgtaaaa gcttgcggga tagtggcaga
     9961 aagtactagc atgattccag aaaagtataa taagacaacc acgattttat gcgcaatttc
    10021 tgttgctaat tgtttaggga taccagaata acgctgatca aaagtggaac cgatattttt
    10081 gaagaaaaag acgattccaa tcaaaaacgg aatgatgtag tagaacaggc gatagagcaa
    10141 gatccacaca acgactattt ctcttggcac accaatagcg gacaacccga taatcatcat
    10201 tacatcgaaa ctcccaattt ctcctggaat catagacacg attcctatca ctgaagcagc
    10261 aatgaataat ggaattgttt gccacagatc aatccggatt tccattaaat aaccaacaga
    10321 aaggaaacta aacaggacac cggtccattc caacaatgaa actagcaata gctgtccttg
    10381 agttttaagt gaggtaccac caaggaatcc tttctttttg aaagtagtaa ataaaaagac
    10441 gacaggaaaa tatagaccgc cgccgatcaa ccatatccaa tattgcttca aaaaaggagc
    10501 aactggcgtg aagactacaa gtaaaaaaga aagcagactg taaatagaaa gtccagccat
    10561 caaaaacaag aaaactttcg ataatgcttg gatcacgttt ctgctctctt tcttttgtcc
    10621 atatagttca gagcgaagcc ccacactaac aaacccgccg aaaccggcaa tattgttgat
    10681 cgtattgatc agccagcttg tctcaaacag gtaacgagga ttctgctttt gttcaagcat
    10741 ccgattcaaa ataatatcat aaccaaccat aggtaacaca gagataagac caatcaatag
    10801 cattagtccg cttttccata caggtatagt ggcaaacgtt tctcctaatt gctggataga
    10861 tagagtttta ccgatggaca tcaattcacc gacaataatg acaatgacag aaatcaaaaa
    10921 gattgttttg aacagtccta ggtggttttt cagccattgt atggtatttt ttaacaaata
    10981 aatcaccttc tattcctaat agttatgttt cagaaagaaa ttgaagaact tctcgattga
    11041 agcgttcagg atcttttcta gctaattcat gtccttgttc tttcacaagc acaaaggaag
    11101 cttttggtat agttttagct atatacaaag aatgttttag cttgattaca tctttttttc
    11161 ccacgatgat caaagtgggg gagttgattt tttttaaatc attttctgta agcccgatgt
    11221 catgcagcaa aagtttgata actaaaagat ttttgcgaag acttgggcga aataaagaga
    11281 gtaaccatac ccatgcataa tgaatattac tgatgactct ggcagaaaaa cgaacgcctt
    11341 taacaagcgt gtttccagaa tttaaaatca atcgatgaac ttttttaggg aagctgctgg
    11401 caaataccag tgctagattt gctccatcac taaatccaag aaaatctgcc tgatcgattt
    11461 tttccagcaa catgatcgta ttcagatcct cggccattaa ctgaaaattc aacatagatg
    11521 cttcgtttgt cgaacgacca tgtcctcggc tatctactag atacacggta tagtagcgtt
    11581 cgagtacagg gacctgttct gaaaaaaagc ggccgctgcc gtcattgcca tgcagtaaaa
    11641 agagagggaa gccttgaccg ctcttttcat agtaaatttc gctcccatct ggcatttttg
    11701 catatttctt ttcttttttc acataatcac ctactgtgta aagtataacg attatatcag
    11761 tcattgggca attgttttta ctgaaaaaca acgatcattt tcgtaagatg tcaaaaaata
    11821 caggaaaatt cattttttct taaggcgtac acagaaatca aggaagaaat agccaagaag
    11881 ataaagaaat agtaagataa aaaaagatgt caaaataact aggtgatgaa atgaagcgaa
    11941 aacgattatc tattatccgt attctttttt acatgatcat tctgacggtt attggtgcgg
    12001 tgttgttttt atgaggaaga acttatcgtt cggtaaaacg agtggaaagc tatcaaatag
    12061 aaataacaac agctgcaaaa aattatcata tggaaaacta cgagaatttg atcaaggcga
    12121 taatattgac cgaatcaaaa ggaaaaggga cagatctcat gcagagctcg gaaagtctgc
    12181 atggagaagc tgagatcatt gataatccag ctgacagtat tgatcaaggt gtcaaatatt
    12241 tggcggaaat gatagtagaa gcaaagaaac aaggatgcga tttatggaca gctgtccaag
    12301 catataattt tgggaaagat tatattgcct atgtaaaaaa tcatggcgga gaaaacacag
    12361 tagaattagc agaagcgtat tctaaagatg tgctgagccc acttttagga aatgaggaag
    12421 ggaaacaata ccgatattgg cggatccagt ccttgcttta caatggcggc tatctctatc
    12481 ataatggagg taacatgttt tatcctgata ttgtcaaaat gaatgaaaaa ataatagaga
    12541 cctatgaaaa agtctttaag taaaatagca ggaaatgttc aatcatgaat aaaattaaat
    12601 gaaagaagga gtgcgcaaat agtggaaaaa aggcaaaaaa tggaaaatca agaagtgggg
    12661 tacacaacca aagactggat gttacggttt gtgaagggaa tgtttatcgg ttcaggattt
    12721 atcttgccag gagtcagcgg tggtgcatta gcagctgtct ttggcatgta tgagcggctg
    12781 atttcttttt tagcccacct tacaaaaaat ttcaaagaaa atgttttgtt ttttctgcct
    12841 gttggtctag gcggagtgac aggcatcttt ctactttctt ttttagtcag ttttttatta
    12901 ggtgcgtatg aaacaacgat attatggttt tttgtcggat gtatcgctgg aactgttcct
    12961 gcgttatgga aagaagcagg gaaaaaagga agagatactt ctgatgtgat catcatggtt
    13021 gttacttttg ttctggctta tttgttttta ttatacggcg ctcaattatt tgacgggcaa
    13081 gtcgatcaga attttggaac atggctgatt gccggcggat tgattggttt gggtatgatc
    13141 gtcccaggat tgagcccttc taattttttg gtgtatatgg gattgtataa agcgatggct
    13201 gacggattta aagaagtacg acttgaagtg attgtgccga tcggaattgg tgggattctt
    13261 tgtgttcttg gattgtcaaa aatcatggat tacattttct caaaggcata tagcaagcta
    13321 ttccatttca ttttaggtgt agtttttgct tcaacgatta tgatcattcc taccgattac
    13381 gcgggattgg gagttgctgg ggtactagct tgtcctgttc tttttatagc cggtacagca
    13441 ctaggttggt ggatgagccg tttggaggaa caatataagt aaagatagat tgataataaa
    13501 aacagcacac gggtgattca aacaaaaagg ttggcatctt ttttgtaaaa tcattcgtgt
    13561 gtttttctat aaattttata aaaacaagtt gtttttaatc ttggaacacg ctagacttaa
    13621 acacgtgatt atttttgaaa gttggcggaa aatggagaaa aagaaaatga atggacttac
    13681 acagcaagag gtagttcaga aaatggcttc tggccagcaa aatgactacc aggaagatgc
    13741 atcaaaaagt acaaaacaaa tattcagcga taatatattg acgttattca attttctgaa
    13801 ctttggtatt gggatttgtc tgttatttgt aggtgcgtat tccaacatgg cgtatttagc
    13861 aattattgtc gtcaatatcg ttattggaat ttatcaagaa atacatgcaa gaaatcttgt
    13921 acggaaatta tcgattgttt cccaatcaga agtactagtg atccgggacg gtaaagaaca
    13981 atcgatttct actaaagagc tggtattagg agatttggta aaaatcggag caggagaaca
    14041 gataccagcc gatttacagg ttatatctgg acgggcagaa gcaaatgaag ctcttttgac
    14101 aggcgaatct gatttgattg tcaaagaaac tggagctgaa ttgctttctg gaagcttttt
    14161 gacaagcggt tctgtcatgg ctgaagttat ccgagtaggt gcagataatt atgctgtccg
    14221 cttgactagc gaggcaaaga ctcacaaagc tattcgttct gaacttgtag attcgattcg
    14281 caaagtatcc aaattcacta gtttcgtgat tattcctttg gggatcatac tatttttgga
    14341 agcactgttc atccgcgatg cttctatcaa aatttctgtt attgcatctg cagcagcttt
    14401 gttaggcatg cttccaaagg gtttggtatt gctaataagt attgcattaa cgacaggggt
    14461 aaccaaactg gcaaaaaaac gaatccttgt tcaagacatg tattcaattg aaacgctggc
    14521 ccatgtcgat acattatgct tggataaaac agggaccatt actgaaggga aaatgagtat
    14581 ccagcgggta gaagcactac atccgctgta cgagaaagcc atcccagaaa ttatggggac
    14641 gtatctggca gaaagcagtg ataacaacat cactatgcgg gctatgagag attattttga
    14701 gctttctaat cggtatgatg tgacaaacaa agtcgcattt tcttctgatc gcaaatgggg
    14761 cgcgatggaa tttccagaac tgggagtcgt gtacgtagga gcgcctgaac gtttggtgga
    14821 agaagatcgg cttccggaca gtctaaaaac agcccaagcg aatggctatc gcgtacttat
    14881 gttggggatc gctccaaatc agcaattgaa cgaagaaaaa ccagcatttg ttgagccggt
    14941 tgctctgttt gaaatcgatg atccaatccg aaaaaacgct caagaaacac tagcttatct
    15001 tcatgaggaa ggtgtggatc tgaaagtgat ttctggagac aatcctttta ctgtctccaa
    15061 tatcgctaga cgtgcaggat taccgaatta tgatgcgtat gtcgatcttt ctcaaattac
    15121 tgaagaaatg gaagtgcgag aagctgctca tcgctatacg gtttttggac gagtgagtcc
    15181 gcagcaaaag aaattgctgg taaatgaatt gaaagaaagc ggcagaacgg ttgcaatgac
    15241 aggcgacggt gtgaatgatg ttttggcgtt aagagaagca gattgcagca ttgctatggc
    15301 agaaggtgat ggagcaacac gtcagattgc gaatctggta ttgttggact ctgattttac
    15361 tacattgcca gaagtcctgt ttgaaggacg ccgagtggtc aacaatgtga cgaaagtctc
    15421 aggcatcttt tttatcaaaa cgatttattc atttatttta tcgattatat gtgccgttac
    15481 agcaattgga tttccgttta tccctatcca aatcacgctg attgatttag cgattgaagg
    15541 gtatccttcc ttctttttat cttttgaaca agataaaaga aaaatcactt accgttattt
    15601 gcctacagca cttattaatg ctttaccaaa tgccttgctt gtagtagtga atatcattgc
    15661 ggtgtatcta cttggaaaaa cattcgcatg gggaaatgct gaaactacta ctttgatgta
    15721 ttacttgctg attgggatca gttgcatggc agttgtaaaa gcttgttatc ccttcaaccc
    15781 attgcgcatc ttcctagcag taacgacgat cgtagggata tatgtagctg caatgctgtt
    15841 ccatggaatt ttggaagtaa acttagggtt gggagagagc gtaggatact tctttgtatt
    15901 catgctcgta aatattgttc ttcgagtatt gtactggaag gctgatattc aaaatcagtt
    15961 actgaaaaga gtagcaaatt gatgcttaat gagatagaaa tgtgttatac ttgaaaaagc
    16021 aatgaaatat gatacaatca aacatgtata attgtgtcct ttaaaatcgc actgatttca
    16081 gttagtgcga ttttttaccg gttattctcc tgaaatatct tctgttaaac ccaaaagata
    16141 tcaatagagc agggggtgaa aaaatggctc aacggattcc tcaagaggtt atcgaaacag
    16201 tacgcagtca aacgaatatc gttgaggtga ttggacaata tgtccagttg aaaaagtctg
    16261 gtaaaaatta cttggggctg tgtccttttc atgaagaacg tacaccgtct ttttctgttg
    16321 cagaagacaa gcagattttt cattgctttg gttgtggtaa aggcggaaac gtgtttactt
    16381 ttcttcaaga actagaaggt ctaagttttc cagaagcagt ggtaaaagtg gcagacttcg
    16441 aacagatacc gttagaagat caatggcgtc agcgtactat agctgtccaa aatcctgaat
    16501 ctgctacggg gaaactgatt gctgctcatg aaaaagcagc ggaaatctat catcacatgc
    16561 tattgcatac aaaagctgga cagccagctt tagaatattt gcaaagtcgt gggttgtctt
    16621 tagaattaat tgaagaattc aacataggtt ttgcacctaa tgagcgttct tttcttcagc
    16681 aagtattcaa gaatgaatca atttcaaatg aattgttaga acgctctgga ttgtttgtca
    16741 cgcatgatga tggccgtatg tctgatcgtt tttatcaaag agtgatgttt ccacttcgta
    16801 attctcaagg ccagacgatc ggtttttcag gacgctggct tgcacctgaa aaagggcagg
    16861 aaaaagatca gcccaaatac ttgaatagtc cagaaacaga actgttcaat aaaagacaag
    16921 ttttatttaa tcttgataaa gcaagaagcg atatccggaa aaacggagaa attttgttgt
    16981 tcgaagggtt tatggatgtg attgctgcat ggcaggctgg tatcaaaact ggattagctt
    17041 ctatggggac aagtctaaca gctcaacaga tccaacaaat ggaaaagctg acgaaagaat
    17101 tggtcttttg ctatgacgga gacaacgcag gttttgaagc aaccaatcga gggattgaat
    17161 tgcttcggca aaacagtcga ttagcacttt ctgtcgtagt cgttccagaa aagcttgatc
    17221 ctgatgaata tctccgtaag tatggagaag catctttcca agaattagtg gctcatgggc
    17281 gcgaaacagt ctttactttt aaatcacgtt atttaaaaca agggaaaaat ttagataatg
    17341 aaaaagacaa gatcgaatac ctcgaagaat tactagttga acttagccga gttgtttcac
    17401 caatcgaaca agatatgtat ctatctcaac tgtcaacaga gtttcagatc ggcagagaga
    17461 cgatacaaaa acaactgcgc gatcttcgaa ggacaaatca gcaaaatcgt caagaacctc
    17521 aggaaagtca acacactcag gttacgagac aagaaactgc acggaaaaag agacctttga
    17581 cgcaagtaga aaaggcagag cagattctat tatatcgtgc atttaaagaa gtttctgtcc
    17641 gtaatatttt aaaagaacga gaatttcagt ttattcatga tgcatatgca gaagtttatt
    17701 ttttattcga tgcatatgtc tcacaacatt atgaattcaa ccttgcagag tttttagact
    17761 ttttgcaaga tgaaaattta aggcggttgg tggtggaaat cgaatattta ccggtggcag
    17821 aagaaagttc gccacgagaa atccaagact tgctagatgt gatcagcaaa tctggcttag
    17881 ctgatgaaat aacattaaaa aaacaaatgc agcaagaagc cagacgaact ggaaacaaac
    17941 agcttgagct tgaattgacg attgaaatca tcaatttaac gaaacagtta aaacaagcca
    18001 aatagctact caaggggggc cttcttttat ggcaaatgga acagataaaa aaacgtatga
    18061 agcagcagta gcagctttca tcaaagaaaa caaacccaaa cgacaagttg tatatgatga
    18121 tttatccaat cagcttgcta caccattcac tttgaatgca gatgaaatgg acaaattgat
    18181 ccaaaagatt gaagacgcag gtatcagtgt ggtggatgaa aatggtgatc catcaattca
    18241 cagcttgaag agtgctgaga aaaaagctga acaagcaaaa acagaagatc tttctgcgcc
    18301 aaccggtgta aaaatcaatg acccggttcg tatgtatctg aaagaaattg gccgtgtctc
    18361 ccttttaact gcggaagaag aagtagcgtt agcattgaaa atcgaagaag gcgaccaaga
    18421 agcgaaacag cgtctggcag aagcaaacct tcgtttagta gtaagtatcg ctaaacgtta
    18481 tgtaggacga ggcatgcagt tccttgactt gatccaagaa ggaaacatgg gactgatgaa
    18541 ggcagttgaa aaatttgact accgcaaagg atttaagttt tcaacctatg ctacatggtg
    18601 gattcgtcaa gccatcactc gagcaatcgc tgaccaagca cgtacgatcc gtattccagt
    18661 tcatatggtt gaaacaatta ataaattgat acgtatccaa cgtcaattgc tgcaagacct
    18721 tggaagagaa cctacgccag aagaaatcgg tgcggaaatg gatcttccaa cagaaaaagt
    18781 ccgagaaatc ttgaaaatcg cgcaagaacc tgtttccctt gaaacgccaa tcggtgaaga
    18841 agatgactct cacttaggtg atttcataga agaccaggaa gcaacaagtc cagcagaaca
    18901 cgcagcttat gagttactaa aagaacaatt ggaagatgta ctagatacat tgactgatcg
    18961 ggaagagaat gtccttcgct tacgttttgg tttagatgat ggccggacga gaacattgga
    19021 agaagtaggg aaagtatttg gtgttactcg ggaacgtatt cgtcagatcg aggctaaagc
    19081 attaagaaaa ttgcgccatc cttctcgttc aaaacaatta aaagatttct tagaataagc
    19141 gtggactatt gaccagaaga gaaatcagta aaactgtttg gtttatttta aaagaaaaa
//
LOCUS       DAFCNJ010000051        19126 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000051 DAFCNJ010000000
VERSION     DAFCNJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.052"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..703)
                     /locus_tag="LNE35_002126"
     CDS             complement(<1..703)
                     /locus_tag="LNE35_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6911250.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="LNE35_002127"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="LNE35_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="LNE35_002128"
     CDS             1758..2837
                     /locus_tag="LNE35_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6911251.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="LNE35_002129"
     CDS             2869..3462
                     /locus_tag="LNE35_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6911252.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="LNE35_002130"
     CDS             complement(3572..3799)
                     /locus_tag="LNE35_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6911253.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="LNE35_002131"
     CDS             complement(3902..6490)
                     /locus_tag="LNE35_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBK6911254.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="LNE35_002132"
     CDS             complement(6510..7169)
                     /locus_tag="LNE35_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6911255.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="LNE35_002133"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="LNE35_002133"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBK6911256.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="LNE35_002134"
     CDS             complement(7942..8808)
                     /locus_tag="LNE35_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6911257.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="LNE35_002135"
     CDS             8917..9558
                     /locus_tag="LNE35_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBK6911258.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="LNE35_002136"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="LNE35_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBK6911259.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="LNE35_002137"
     CDS             complement(10999..11898)
                     /locus_tag="LNE35_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK6911260.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="LNE35_002138"
     CDS             complement(11900..12697)
                     /locus_tag="LNE35_002138"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBK6911261.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="LNE35_002139"
     CDS             complement(12675..13379)
                     /locus_tag="LNE35_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBK6911262.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="LNE35_002140"
     CDS             13510..14085
                     /locus_tag="LNE35_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBK6911263.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="LNE35_002141"
     CDS             14203..14880
                     /locus_tag="LNE35_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK6911264.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="LNE35_002142"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="LNE35_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBK6911265.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="LNE35_002143"
     CDS             complement(16943..17941)
                     /locus_tag="LNE35_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBK6911266.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="LNE35_002144"
     CDS             complement(18167..18820)
                     /locus_tag="LNE35_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBK6911267.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DAFCNJ010000052        18740 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000052 DAFCNJ010000000
VERSION     DAFCNJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.053"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            256..531
                     /locus_tag="LNE35_002145"
     CDS             256..531
                     /locus_tag="LNE35_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911268.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="LNE35_002146"
     CDS             544..2616
                     /locus_tag="LNE35_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBK6911269.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="LNE35_002147"
     CDS             2781..4667
                     /locus_tag="LNE35_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6911270.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="LNE35_002148"
     CDS             4679..5626
                     /locus_tag="LNE35_002148"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBK6911271.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="LNE35_002149"
     CDS             5646..6173
                     /locus_tag="LNE35_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK6911272.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="LNE35_002150"
     CDS             complement(6236..6889)
                     /locus_tag="LNE35_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBK6911273.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="LNE35_002151"
     CDS             7021..7863
                     /locus_tag="LNE35_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6911274.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="LNE35_002152"
     CDS             8021..8917
                     /locus_tag="LNE35_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK6911275.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="LNE35_002153"
     CDS             8920..9633
                     /locus_tag="LNE35_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBK6911276.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="LNE35_002154"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="LNE35_002154"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK6911277.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="LNE35_002155"
     CDS             10168..10395
                     /locus_tag="LNE35_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBK6911278.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="LNE35_002156"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="LNE35_002156"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK6911279.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="LNE35_002157"
     CDS             complement(11181..11681)
                     /locus_tag="LNE35_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK6911280.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="LNE35_002158"
     CDS             complement(11960..12358)
                     /locus_tag="LNE35_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6911281.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="LNE35_002159"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="LNE35_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBK6911282.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="LNE35_002160"
     CDS             13471..14238
                     /locus_tag="LNE35_002160"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBK6911283.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="LNE35_002161"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="LNE35_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBK6911284.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="LNE35_002162"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="LNE35_002162"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBK6911285.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="LNE35_002163"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="LNE35_002163"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBK6911286.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DAFCNJ010000053        17150 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000053 DAFCNJ010000000
VERSION     DAFCNJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.054"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            138..1475
                     /locus_tag="LNE35_002164"
     CDS             138..1475
                     /locus_tag="LNE35_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBK6911287.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="LNE35_002165"
     CDS             1523..3652
                     /locus_tag="LNE35_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBK6911288.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="LNE35_002166"
     CDS             3627..4316
                     /locus_tag="LNE35_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911289.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="LNE35_002167"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="LNE35_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="LNE35_002168"
     CDS             complement(5546..5740)
                     /locus_tag="LNE35_002168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911290.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="LNE35_002169"
     CDS             complement(5790..6230)
                     /locus_tag="LNE35_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBK6911291.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="LNE35_002170"
     CDS             complement(6234..7079)
                     /locus_tag="LNE35_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBK6911292.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="LNE35_002171"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="LNE35_002171"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBK6911293.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="LNE35_002172"
     CDS             complement(8703..9650)
                     /locus_tag="LNE35_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6911294.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="LNE35_002173"
     CDS             complement(9873..10223)
                     /locus_tag="LNE35_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK6911295.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="LNE35_002174"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="LNE35_002174"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBK6911296.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="LNE35_002175"
     CDS             11646..11966
                     /locus_tag="LNE35_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK6911297.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="LNE35_002176"
     CDS             11988..12452
                     /locus_tag="LNE35_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6911298.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="LNE35_002177"
     CDS             12657..13262
                     /locus_tag="LNE35_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBK6911299.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="LNE35_002178"
     CDS             complement(13301..14590)
                     /locus_tag="LNE35_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBK6911300.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="LNE35_002179"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="LNE35_002179"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBK6911301.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16250
                     /locus_tag="LNE35_002180"
                     /pseudo
     CDS             15700..16250
                     /locus_tag="LNE35_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            16321..>17150
                     /locus_tag="LNE35_002181"
     CDS             16321..>17150
                     /locus_tag="LNE35_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HBK6911302.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT         5459 a         3105 c         3467 g         5119 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttgaagaga gtgtaaaata ttttgtgtaa atagaaaaaa
    16261 ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat
    16321 atgaacgatt ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat
    16381 ttgtttcgtc gtcacctaga attggctatt aacgctttat tacaagctga attaacggta
    16441 tttcttgact atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg
    16501 aattattcgc gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat
    16561 aggaatggag acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
    16621 gaaaccacta ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta
    16681 atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt
    16741 gtgtctgaag atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt
    16801 tttatggatg ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat
    16861 atagtaattg gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca
    16921 actgaatctg cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa
    16981 gaggttttgt tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt
    17041 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
    17101 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca aattagttta
//
LOCUS       DAFCNJ010000054        17050 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000054 DAFCNJ010000000
VERSION     DAFCNJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.055"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            205..2676
                     /locus_tag="LNE35_002182"
     CDS             205..2676
                     /locus_tag="LNE35_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6911303.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2779..4515)
                     /locus_tag="LNE35_002183"
     CDS             complement(2779..4515)
                     /locus_tag="LNE35_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBK6911304.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4512..5216)
                     /locus_tag="LNE35_002184"
     CDS             complement(4512..5216)
                     /locus_tag="LNE35_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6911305.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5347..5850
                     /locus_tag="LNE35_002185"
     CDS             5347..5850
                     /locus_tag="LNE35_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBK6911306.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5810..6148
                     /locus_tag="LNE35_002186"
     CDS             5810..6148
                     /locus_tag="LNE35_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBK6911307.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6169..7587
                     /gene="ffh"
                     /locus_tag="LNE35_002187"
     CDS             6169..7587
                     /gene="ffh"
                     /locus_tag="LNE35_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBK6911308.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7699..8277
                     /locus_tag="LNE35_002188"
     CDS             7699..8277
                     /locus_tag="LNE35_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBK6911309.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8281..8802
                     /locus_tag="LNE35_002189"
     CDS             8281..8802
                     /locus_tag="LNE35_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK6911310.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8881..9435)
                     /locus_tag="LNE35_002190"
     CDS             complement(8881..9435)
                     /locus_tag="LNE35_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK6911311.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9688..9963
                     /gene="rpsP"
                     /locus_tag="LNE35_002191"
     CDS             9688..9963
                     /gene="rpsP"
                     /locus_tag="LNE35_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBK6911312.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9975..10226
                     /locus_tag="LNE35_002192"
     CDS             9975..10226
                     /locus_tag="LNE35_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBK6911313.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10351..11142
                     /locus_tag="LNE35_002193"
     CDS             10351..11142
                     /locus_tag="LNE35_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911314.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11312..11833
                     /gene="rimM"
                     /locus_tag="LNE35_002194"
     CDS             11312..11833
                     /gene="rimM"
                     /locus_tag="LNE35_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBK6911315.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11823..12584
                     /gene="trmD"
                     /locus_tag="LNE35_002195"
     CDS             11823..12584
                     /gene="trmD"
                     /locus_tag="LNE35_002195"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBK6911316.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12720..13067
                     /gene="rplS"
                     /locus_tag="LNE35_002196"
     CDS             12720..13067
                     /gene="rplS"
                     /locus_tag="LNE35_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBK6911317.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13199..14335)
                     /locus_tag="LNE35_002197"
     CDS             complement(13199..14335)
                     /locus_tag="LNE35_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6911318.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14451..15365)
                     /locus_tag="LNE35_002198"
     CDS             complement(14451..15365)
                     /locus_tag="LNE35_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBK6911319.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15456..15884)
                     /locus_tag="LNE35_002199"
     CDS             complement(15456..15884)
                     /locus_tag="LNE35_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK6911320.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15889..16263)
                     /locus_tag="LNE35_002200"
     CDS             complement(15889..16263)
                     /locus_tag="LNE35_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6911321.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16549..16734
                     /locus_tag="LNE35_002201"
     CDS             16549..16734
                     /locus_tag="LNE35_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6911322.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16746..16994
                     /locus_tag="LNE35_002202"
     CDS             16746..16994
                     /locus_tag="LNE35_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911323.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
BASE COUNT         5543 a         3046 c         3343 g         5118 t
ORIGIN      
        1 gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg attttccacc aacacaaaat
       61 attatagaac cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg
      121 tgaggaacac tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa
      181 aacgaaatca atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag
      241 aaagtaacac aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga
      301 ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat
      361 cccacattca aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg
      421 atctttctta tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt
      481 ttaccagtta tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag
      541 atcttggacg aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata
      601 gaacaaacaa tagatataga agaccttgta atagatgaca ttgttatttt agagactgga
      661 tatcaaattc cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta
      721 ttgacaggcg aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt
      781 ttcattgttt ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt
      841 tcccagctga cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct
      901 tctcttaatc aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt
      961 ttttctcaaa gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa
     1021 gcagcattga tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca
     1081 ttaagtgcga ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag
     1141 acattggcaa gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa
     1201 atgtctgttc aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa
     1261 ttggaagcat gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa
     1321 gctgttaaag catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt
     1381 tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg
     1441 cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct
     1501 gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta
     1561 ttgacagaag cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa
     1621 gcaagagcca catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga
     1681 gataatccgc tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag
     1741 tacgttgatg catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca
     1801 gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac
     1861 aatacagtag ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac
     1921 tgcagtatag cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta
     1981 cttgattctg acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac
     2041 aatattgagc gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca
     2101 ctgttttcag tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt
     2161 agtttgttta ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga
     2221 atcagaggga aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat
     2281 atgatcgtag tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat
     2341 gtttctacag catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc
     2401 tgttcaccgc tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt
     2461 ttttcggtca tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt
     2521 ttgctgttga gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg
     2581 gtagaatatt tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct
     2641 aaaaaatttc tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta
     2701 aaaaagaaca accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc
     2761 acgagcagtt gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc
     2821 aagatgactg tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc
     2881 taaccctaat ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg
     2941 ctcaaagatc cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa
     3001 cgatagatca tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg
     3061 gacagcattt tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa
     3121 tggttcgttc tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata
     3181 attttgaacg atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt
     3241 ctcgccatct tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc
     3301 tttttgcatg atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag
     3361 taaagtctcg gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt
     3421 gccaacgaaa tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac
     3481 ccctagaatc tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt
     3541 ctgccgacct tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg
     3601 atgaatcagc tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg
     3661 atagcttacc acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc
     3721 agcatcatca atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc
     3781 ttctgtggta gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt
     3841 ctgatacaat tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt
     3901 ttgcaccatt ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat
     3961 cagataatag atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca
     4021 acggcgaaaa ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat
     4081 ggcttctaac tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa
     4141 agtagtactt ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc
     4201 gggacgggag tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc
     4261 agtagcatcc attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt
     4321 tttgttttgc gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt
     4381 ttttgtcagg tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa
     4441 ttgccagcca ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat
     4501 ttttttccat ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc
     4561 aagtactttg ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca
     4621 tctacgattc ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct
     4681 cggtcgatca cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt
     4741 gttagttcaa tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt
     4801 tgtccaatcg atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt
     4861 cgtctgaata ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa
     4921 tagtcgtcag cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc
     4981 agcatcaaaa tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca
     5041 tccatcgttg gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat
     5101 tctaatcctt cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg
     5161 aacgtcaaga gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa
     5221 atctccttta aataggtttc attctgttta tattttagca tagaatcagt acaaagggct
     5281 attttacaaa agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag
     5341 cagtcaatga ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag
     5401 aaaaaagaag taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt
     5461 ccagaagtga tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat
     5521 ggattcgatg tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg
     5581 gaagcgtaca aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat
     5641 ctagtcatca tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt
     5701 ttttccggaa taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa
     5761 atgactgtgc tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga
     5821 aaagaccaat cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca
     5881 aatgaactac atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga
     5941 atacgaagta agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga
     6001 ggattatgaa aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga
     6061 aaatatgact gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt
     6121 acagcaaatt caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa
     6181 agcttaactg aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca
     6241 gaagctgacg taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt
     6301 aacttgcaag tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa
     6361 gtgctggaaa gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg
     6421 atcacacttg gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc
     6481 atgatggctg gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat
     6541 ttaaagaaaa atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct
     6601 gcgatcgacc aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga
     6661 actgaagtca gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa
     6721 aacgactatg tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat
     6781 gagttgaaac aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc
     6841 atgactggtc aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca
     6901 ggggtcgtca tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc
     6961 tcagtgacag gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa
     7021 gtattccacc cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg
     7081 attgaaaaag cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg
     7141 cgggaaaatt cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg
     7201 ggcccactgg aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa
     7261 aacgtcaagg tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg
     7321 ccagcagaaa gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc
     7381 ggttctggaa acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga
     7441 aaaatgatgc agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt
     7501 ggttcaggcg tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat
     7561 aagaagaaaa agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa
     7621 tgtctactgc cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta
     7681 aacgaagagg tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa
     7741 tatcatgata ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg
     7801 atgcttgata ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca
     7861 ttcgatgaag catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa
     7921 atcagtgaac taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc
     7981 gtccaaaacg ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat
     8041 ctttggtcgt tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc
     8101 ccaacaacaa gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa
     8161 tttatcggaa gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac
     8221 cttgacagtt gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc
     8281 atgcgaaaaa tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg
     8341 gatgatcttc aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt
     8401 tatgaaacaa tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat
     8461 ttggagaccg aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa
     8521 ttacaattgc cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta
     8581 caaatgacag aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta
     8641 ttggaacatc aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac
     8701 gggattcctt tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca
     8761 cgattcggtg aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa
     8821 aggcaactat atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt
     8881 ttactctgtt aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact
     8941 taatgttcct tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata
     9001 tgcttccggt tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg
     9061 gtttgcgcca aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg
     9121 atcccaaaca ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc
     9181 tggcacagaa cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc
     9241 ctcacgaaaa cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta
     9301 aaatggcaga tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc
     9361 aaaagcttct gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc
     9421 aatttttttt gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga
     9481 taagtaactg gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact
     9541 tatttttaac aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc
     9601 aagctgtaaa gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga
     9661 ataaaacaat ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt
     9721 tctaaaaaga gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga
     9781 cgtttcatcg aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta
     9841 aaagaagact tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt
     9901 aacatccttt caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa
     9961 taaggtgacc ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag
    10021 tcacttatcc tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca
    10081 atctaacagt ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta
    10141 aagcaatccg cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga
    10201 atatcatcga tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc
    10261 ttttttgttt tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt
    10321 atccttatag gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt
    10381 gctctattgt taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca
    10441 agcagtatat ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc
    10501 actgaacaaa caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa
    10561 gaacaacagc caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat
    10621 atcgcagcga cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc
    10681 ctcgggttga atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa
    10741 tatgggtttg cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca
    10801 aatggtcagc aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt
    10861 tcaagttttc ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata
    10921 gaaggccaag atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt
    10981 ctaccagcac ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga
    11041 gccgtagaag taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct
    11101 atgtctgcgt tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg
    11161 agcccatcat ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc
    11221 ataattttgg cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat
    11281 aaaacagaca aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat
    11341 cgtaaacaca caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga
    11401 agaacgttac aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga
    11461 attaacagtc aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca
    11521 tccttctatc aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa
    11581 ctctgtagat ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt
    11641 agaagatgga actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt
    11701 ttgggtcgtt caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt
    11761 caaagaagtc gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga
    11821 cgatgaagat tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat
    11881 ccatcatcgg taaggctgta gataaaggat tattagatat caatatctct aacttccgcg
    11941 attattcaga taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta
    12001 tgctgctcaa agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg
    12061 atgtcaaaaa aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg
    12121 cagaagaatt ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg
    12181 atgaacgtat ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg
    12241 ggggagaatt aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt
    12301 tagggaatca gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc
    12361 aatatactcg cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa
    12421 atcataaatt gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc
    12481 ggcctgatct tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa
    12541 tcaagaaaga agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt
    12601 ctttacagta ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat
    12661 tacgatattc cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca
    12721 tgaatccatt aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc
    12781 gccctggtga cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc
    12841 agttatttga aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag
    12901 tacgtaaagt ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg
    12961 ttgcaaaaat cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac
    13021 gtgcattaca cggaaaagct gctcgtatca aagaaatccg tcgttaatca tcgttatcag
    13081 actgagaaag aacccttggt attcaagggt tctttttttg ttctctgttt ttttatatta
    13141 acgatttcta tttgggacat ttttgattaa taaaaagggg caaaaaaggg gcaacaagtt
    13201 acagtttttc tatatcttct ttcaattttt cttttactcc ctttgtcaca tgcaaataaa
    13261 tcttttcagt gatatcacta ttttcgtgcc caacgcgatc ctgaatagca tacagaggag
    13321 ttcctagttc cgctaatttt gaaatatggg tatgtctaaa aatatgtgaa ctaagttttt
    13381 tatcaatctt catgtcagct ttatgatttc tcaaataggt gttaattgct gttagttgga
    13441 aaggagttcc tttcgttgtt tgaaatagaa attgaccatt cggatttaat tctagaagct
    13501 cattgtaaat agctatcgct ttttttggta aatcaatttc tcgcattcca gcagcagttt
    13561 ttgtagaatc agattttttc atatcagcta ttgaacgttc tctatacatc atcgtcccat
    13621 ttataactac tgatgcatta ttattggtaa tgtgtacatc gtctttactt aatgcgattg
    13681 cttctccagg cctcattcca gtcaaatata gccactggaa aaggagagaa tatcttttat
    13741 tgtgtgaagt tgtgaaattt actagtcttt tatattcatc atcttctaaa aacttatctt
    13801 taatttttat cgtttttgac tctcgtttat aatcaataac tacttcgttg ataggatttt
    13861 tttctacata gccttttttc atagcgtatg aaaaaagaag attcaatttg gatttaatta
    13921 cactgacgta cttatttgac aagtcatctt tatatatcat atcttcaaaa atattattta
    13981 aatctactgt attaatacca gaaacgatgt aagtctcagg tatctttttc tttatagtgt
    14041 ttaagatatt attcgtagga taatatgtag actccttaac ttgtcttttg taaataacta
    14101 accattcttc gacaagctca tgaaatgtta gatcaggctt ctgaagtgtt ttttcgttta
    14161 attttatatc aattttttta tttaactcta gcaatgccac tttctgagtt tctcgagatt
    14221 tatttttata agtaatgctc actttttttc tttttctagt ttttggatca atatatcgct
    14281 cattatattt atatactttt tttccgtttt tatcagtttt ggtttcaatc cacattgttt
    14341 tcatctccta tttgttgcta taataggaat ggataagtaa gccaattata gcaggtttat
    14401 ttttcatcac gtccacaaac tttggcgagg gagggggcgt gtttttttgt ttagtagcta
    14461 cctttccatg aagtatctgc gaagaacact tcaaatgttg ttccatcatt tttcacttca
    14521 aaatatgctg ttccttgtgc ggatttaccc ggttggatgg tttccgaaaa gtaatctcta
    14581 ctagctaaat cagatttaat atctgatgaa tcataaaatt ctaaataact tgaacttgca
    14641 tcaaaaggtg tagtccctgt gttttctaca agaaaatcta cctttgcgta aaataggcct
    14701 tctggtttat agaaatcatc accgtaatct tttgatacag actggacagt tacgttaata
    14761 gagttaccat tttcagaagt aaaaggaact gcttctccta tactatgtgc aactgatgcc
    14821 gtctggggtt ctgaagacga agttgtagtt gtagttgtag tagagcttga atctttggat
    14881 tttccatctt tatctaagaa agttcgttga ttataatata caacctgttc agtttttgga
    14941 ctaacccaaa ttaacattgt ttcatcatca gaatcagttg aataagtata ttggagcatc
    15001 tctaattttt ttatatcttt tgcactattt ccatgttcaa ttaattcaga gtgctcttta
    15061 taataatctc cttgtgcatc ctttgaaata gatacaatta attcatcccg tgtgatagca
    15121 ttgtctaatt ctttgtaaac aaaatcattt gtccatttac gtaacggtga accaagtgtc
    15181 tgataaactt gagttaaatc atcaccaact tttattttat gaacgtctga tgaagttagt
    15241 ttttttgttt catattcaga tgttgatgaa acagtatttt ctttagtgct agcagaatta
    15301 ggactagtgg agcatccgct taataccaag cctaaaaaaa gcaacccaaa aacaattttt
    15361 ttcattttta attcctcact tctatgatat gatttttttg taggatctta gaaataaggt
    15421 ttttagtccg tgttgcagca cggacttttt ttacattata taagaaagcg ataagcgctt
    15481 tctggaagtc cgtaaagatt ctttaattcc tcaattcgtt taggatattg atcattgtct
    15541 tctttgtata gagaaacaat gagattagca gcaaagcaat tagcttcgct ttcagatttg
    15601 cttctagatg ttcttgttga tacatagtaa ctggataagc cacgatgaaa aatagcgtga
    15661 cctaattcgt gagcgcaaat gtagaatctt tcctcagagt ctctcagttc atcatttaag
    15721 aagattattg cacgacctct aatttcttga aactgtcctt tgggattctc gataaaagga
    15781 acgtattgaa ttttaatgcc catcttttca caaatataaa aaggattagc ggactggtat
    15841 ttccgcttca actcctcgac taaattaatc gtatccatct ccataagctc acatcttttt
    15901 gcctttttct ttgtcttctt tcacaatatc ccagaaggtt ccaataagga tatcttttac
    15961 gcgttggacc tgttcaggcg tcagtgtttc tccgccataa gacatattga catttgaatc
    16021 tagtaattta tcaagttcaa ctacttcatc ttttgttgcc cagtcaggaa cttggtttct
    16081 accgagtaaa taatcagtag ttacattaaa ataatctgct acaagttgta gtcttttagt
    16141 actaggagtt ctatttttcc attgataaat cgtgttttta ggtatattta attcttcttc
    16201 caattgagtt atacttatgc ctcttttatg agctaattcc tttattctat ctagtaaatt
    16261 cattttcgca cctcacaagc tacgaaaaca ctaataaaaa aattagttaa aggtgttgac
    16321 aactaataaa agtgttagta tactgttttc gtaagctaaa atattagcta aaaagacttg
    16381 ataactaata aacacttcac ggtcggcaaa ctctgaaatg taaattacta ggcatttctg
    16441 tgtcttattt agctatgtct atatactaat aaaaatatta gttattgtca acgaatatta
    16501 gctaattttt tagcttacag attatttttt tagaaaggag ctatttttat gtctgagaat
    16561 ttagacttaa aaattcgagc ggagatgaga aaaagaagaa tgactttcaa agaactagct
    16621 gcgcttatcg gtatttcagg agcttattta tcagatattc taaacggcaa tcgtgatgga
    16681 aagaaagcac aacagcatat cgaaacagtg aaaaaaatat tggacatccg ataggggggt
    16741 aggcgatgac aaaactaaaa aaacaagatt ttgtaaaaaa atacaattat tctccatcta
    16801 cttatcaacg tcgaatgtcg gaactaaaaa atacagcaat tttctcagcg gcgtatgaac
    16861 gggtcacagg acaagaagtt tggatcaata cagaattata cgataaattt ttgtctttca
    16921 aatcctataa caggttacgc acaagaaagg taacacctaa agaatttatc gagaagcatt
    16981 tagttgattt ataaaaaaca gaacattttg agaggtgaag gttaatgggt aaattcaaca
    17041 gagcattggt
//
LOCUS       DAFCNJ010000055        16668 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000055 DAFCNJ010000000
VERSION     DAFCNJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.056"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(157..864)
                     /locus_tag="LNE35_002203"
     CDS             complement(157..864)
                     /locus_tag="LNE35_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBK6911324.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(943..1281)
                     /locus_tag="LNE35_002204"
     CDS             complement(943..1281)
                     /locus_tag="LNE35_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBK6911325.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1392..2354)
                     /locus_tag="LNE35_002205"
     CDS             complement(1392..2354)
                     /locus_tag="LNE35_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBK6911326.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2369..3700)
                     /locus_tag="LNE35_002206"
     CDS             complement(2369..3700)
                     /locus_tag="LNE35_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6911327.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3708..4388)
                     /locus_tag="LNE35_002207"
     CDS             complement(3708..4388)
                     /locus_tag="LNE35_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK6911328.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4607..5086)
                     /locus_tag="LNE35_002208"
     CDS             complement(4607..5086)
                     /locus_tag="LNE35_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBK6911329.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5213..6892)
                     /locus_tag="LNE35_002209"
     CDS             complement(5213..6892)
                     /locus_tag="LNE35_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK6911330.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7440..8942
                     /locus_tag="LNE35_002210"
     CDS             7440..8942
                     /locus_tag="LNE35_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6911331.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8955..9617
                     /locus_tag="LNE35_002211"
     CDS             8955..9617
                     /locus_tag="LNE35_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6911332.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9718..9921)
                     /locus_tag="LNE35_002212"
     CDS             complement(9718..9921)
                     /locus_tag="LNE35_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK6911333.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="LNE35_002213"
     CDS             complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="LNE35_002213"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBK6911334.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="LNE35_002214"
     CDS             complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="LNE35_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBK6911335.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13885..14499)
                     /locus_tag="LNE35_002215"
     CDS             complement(13885..14499)
                     /locus_tag="LNE35_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6911336.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15105..15482)
                     /locus_tag="LNE35_002216"
     CDS             complement(15105..15482)
                     /locus_tag="LNE35_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911337.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15782..16663)
                     /gene="galU"
                     /locus_tag="LNE35_002217"
     CDS             complement(15782..16663)
                     /gene="galU"
                     /locus_tag="LNE35_002217"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK6911338.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4829 a         3593 c         2847 g         5399 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcccttatt ccaaataaat ggtattcaat cagctaactc tcggtattag
      121 ctcagatcaa agcgctgatt tcacaacttc tttattttaa tctcttcggt tgccgccgaa
      181 caataagatc aagcgcaaca gctgcaggaa tgtcgaaagc gctgctgcaa cataagtcag
      241 tgctgctgca aataatacgt gtcttgccat cggtacttct tcttccgtca gtagtccgcc
      301 ttcactcaaa atttggattg ctcgccgaga cgcgttaaat tcgactggta atgtcaccaa
      361 ttggaaaagt aatgctaaag aaaaagcgaa aatcccgata ttgattaacg tagagttcca
      421 gctaagcaat acacctacca taatcaaggg gaacgaaatc atcgaaccaa agttagctgc
      481 tggaacaagt gctgcacgaa tcttcaatgg gacataaccc gaagcatctt gtaccgcatg
      541 tccgcattca tgagctgcta cacctatcgc agcaactgaa gtagactgtg cagttgcttg
      601 agacaaactc aacatcttat tacctgaatt gtagttatct gtgagatctc cggcaatttg
      661 ttgaacaccg acattattta tctgttgact ttgtaatata tattgtgctg cttgtgtacc
      721 agtcacattg tttttgcttc tcacttgatc gtattttcgg aaagtactat tgacgtacgc
      781 tgacgcaatt ccggaaataa ctaatccaag aatgaccaaa agaaatgtcg gatctaaaaa
      841 accatacatt ccataaccat acattttcgt ttcactcctt ttcgtttact ttattttatc
      901 attagtagta ggaaaagaat gtgaaaagac cttgaatatt ctttaattct ttttcacaaa
      961 ttccgatttc agtttcatcg gtccaaatcc ttctaccttg caatcgatat tatgatcgcc
     1021 ttctactaaa cggatgttct tgacttttgt tccttgttta atggcacctg acgcaccttt
     1081 tactttcaag tctttaatca ctgtcacgct atcgccctct aacagacggt ttccatttgc
     1141 atctaatacc actaatccgt cttctatgac agtttcttgt tcttctttgt tccattcgtt
     1201 cccgcattct gggcaaatca acaagcctcg atcttcatat gtgtactctg agccacattc
     1261 tgggcaattt ggtaatgtca tcttctttct cctttaaaat ctttagtgag cactattgta
     1321 acggaaaaag aagatcttga aaagatacat ttgtgatttt tactacaaaa ggcacgtttt
     1381 cgaacgaagt atcattctgt ttttctgctc ttatacgatt ggcatatatc ttggatctct
     1441 ttatagattt cctctacttc tgctaaatct tttgcattcg caccacttgc taatggatgg
     1501 cctcctccat gatggcgttt agccaattca ttgatgatcg gccccttaga acgtaaacgt
     1561 acgcggtaat accctgaagg ctgctgaaca aaaatccccc aagccagcac ttcgtcaatc
     1621 actccaggaa gtgaaacgat cgaagctgtc tctgaatcat cgattccata tttatcaaga
     1681 atttcttgcg gaaggattat tcttcctgca ccattttcat ccacctggat attttgataa
     1741 agataaccgg aaagtttagc gactttcatc ggcatttgtt caatctctct gttcaaagca
     1801 cttgcatcaa atggataatt tctcaatatc gaagcaatct ctaatgtacg ggacgtcgtg
     1861 gagggataca aaaagcgccc tgtatctccg acgattcctg cataaagtaa ccgtgcagcc
     1921 ttaacgttca tctttaaaga ttcaggaaac atcaaagcaa attctgcaat catctcacta
     1981 cagctacttg cttttgtatt tacccacaca agatctccat aagggtcatc attgggatga
     2041 tggtcgattt tgatcagttt ttctgccaac tgatatcttt gatcactgat tctgggtgaa
     2101 ttagctgtat cagtcacgat gactagcgct ccttggtaga agacatcttc aacttcttgc
     2161 atttctgcta aaaagcgaag cccttcaatc gtttcaccta cttgacgtat ttctttttct
     2221 gggaaacttt cccgtaatag ttcggctaat cctacttgtg atcctaatgc atcaggatca
     2281 gggcgcaaat gcctgtggat aatgatttta tcgtattttt ttattgtatc tactatttct
     2341 tgctgtactg tcatttcact cctgctttct aagaacgttc cattacttga caaacaacga
     2401 ttgctttgga aacgatggtg ttttcaataa acacttcgat atccagttta gcggaacgtc
     2461 tgccgatttc caatacacgt ggacgaatgt ctaattcgct ttccaattgg atcagccgca
     2521 aataatgtag gttcacttgt tcaatcaaga tatttcgttt ttggttcatc accattgttt
     2581 tttgtgcgac atcagaaatg atttcgctaa gtacgccaaa agaaatggtt ccaacgccat
     2641 tgaccatttg tggtgctact acgaatttga acttagggtt ttccaaacga tttccttcac
     2701 ggtcagtatc aatcgtatgg atatttccag atatctgatc cgaaatcgta tctgcaatct
     2761 gcggttgtcg ctggacaagc tgcatcgctt tcattacatc ttgccgggta atcaagcctt
     2821 ccaatgtcaa gtcatcggaa acgaccggca tgacttctag cccatcccag atcatttgat
     2881 ggctaacaga ggcaatactc attgttttct ttacactgat tggttctttc gtcatcaccc
     2941 gatcgatgat ctggtttggc gatttcccta aaacatcttt tgccgtaaca atccccatca
     3001 aacgatgatg gcggttgacg actggaaaac gcgaatgctg cgtttcttct gacaaacgct
     3061 gataatcaga aatcgtatct gtctgctgca aataatgagt cttttcagct ggcatataga
     3121 tatcactgac aagcatgata tcttttttga tcaattggtc gcttaacgct cggttgatca
     3181 tggttgcgac tgtaaatgta tcataagagg tgcgtaagat cggcatttca agatcatcag
     3241 ctagctttgc aatctcagca gtcgtattga atcctcctgt gatcaatact gcggcgccat
     3301 cttccaatgc taatttttgg acttccgccc ggttgccgac gatcattagt gatcctggcg
     3361 taatatagcg ttccatggca tgtttagtca tcgcgccgat gacgaattta ttcaagacct
     3421 tgtctaatcc tgccgccccg cctaaaacgt ccccttcaat gatcctgacg acctcgccaa
     3481 atgtcaaacg ttcaatatgt ttttttaatt tgcgttcgat acggatggtc cctactcgtt
     3541 gaattgttga aactagtccg acattttctg catctttgat tgcacggtac gctgttcctt
     3601 cactgacatt caattctttt gcgatgcttc ttacagaaat tcgattgcca acaggtaaac
     3661 tttcaatata ttctaaaatc aaatcatgtt ttgtcgccat tttctgccta cacttccagc
     3721 gtttccgaga cgctcattat ttttccttca ctatcttcca gtaactctac aaaagattcg
     3781 ggatcttgtt caatgacagg gaaagtattg taatggattg gaactacttt cttggcacga
     3841 attgtttgcg cagcctttgc tgcatcttct ggccccatcg tatagttatc gccaatcggc
     3901 aagaaagcga catcaatatc gaattcttct gcaagcaatc ctagatcact gaaataagca
     3961 gtatcccctg catgataaat cgtttttcct tctgcttgaa taataattcc gctaggctcg
     4021 cccatatact gaacgatacc gtctacttca tagcttgaac tatgctgtgc atgtacgaat
     4081 tttaccacgc cgaatggaaa cgcatgtttt ccaccgatat tcataccgaa tgaatctact
     4141 cctctgctgt tcgcataatc agcaatctca acaatactga taattgtggc ttgattttct
     4201 tttgcaatag gaatcatatc tcctatgtgg tcactatgac catgagtaat cagcaaccaa
     4261 tctgtcttta cttcttctgc agttaagtct gtcaatggat ttcctgtaat aaacgggtca
     4321 atcatcacgc gttggccatt ttccatttca attgttaaac aggaatgtcc atgatacgta
     4381 attttcatat tttacacctc gcgttttctc tgtatctttt ttattaaaaa cgatttcatc
     4441 acttgcacat caatgaaaaa atatagctgc taaatatatt ttcattggat tgatagtaca
     4501 gtatgtacaa agcaattgta acagataata caggatgcac caagtaccaa gcagatataa
     4561 tctcaagttt gaggtctctg ctctgcttag cttctcttct cgtcccttaa ctaattaaat
     4621 cagcgtaaat aggatggacg atcttttcta aatcggctac ttttatccga aggcttctcc
     4681 cgatttcacc gcttgacaca attacttctt ctttttcttt taaagaactg tctgctaaaa
     4741 aacaataatc aggatgtgcg agttttattc caataggggt gtttgctccg tggggatatc
     4801 ctgtatgcat taatacaaaa tccattccag gaaaaccaac tttacgatct ttcgtcactt
     4861 gtctcgtttt tttgtaatct aagtgatcgt tcaaaggaat caatgcgatg atcaccccag
     4921 ttttgtttcc tttcactacg agagttttac atatcaaagc tggatcaatc cctctttttt
     4981 gtagttcttc tgtgaaagat tcttctacca catgactaaa atcaagtggt tgatagctga
     5041 tcttttgttc ttctaaataa cgttcgactt ttgtttgact catcattgat tcactccctt
     5101 tattctatcg tttattataa agcgagggat gctgtattca aaaattattt gtttttcgca
     5161 aaaaaagagc aagacgagca ctctatagag aggttcatct tactcttttt gtttattttt
     5221 tatttcgacg ttgagtagct tccataatga ctggcaagat cactgggcgg cgttttgttt
     5281 gttcaaataa gtaacggcct aattgatctc ggatgtcttg ctttaatttg ctccactcaa
     5341 aatcattgct ttctaaatgt ttctcaacga ttgtttcaat catattgctg ctttctttca
     5401 tcagatcacg gctagctttt acgtaaacga atccccgtga agtaatttga ggttttgaaa
     5461 tgattttctt ttctcgccga ctgatagtga caaccgctac gaaaatcccg tcttctgaca
     5521 agatttttcg atcacgtaaa acaatgtttc cgatatcacc tatgcctaat ccatcaatca
     5581 ttacattttc tgcacttgta gttcctgcaa cactcatacg cttgttctta tattccagca
     5641 cgtctccgcg tccagtaata tagatatttt tgtatggcat ccctactgca tgagccaaat
     5701 ctgcatgagc agctaattca cgatactctc cttgaactgg cacaaaatat gttggtttca
     5761 tcaaattgat cattaattgc aagtcattag gattggcatg gccagaaacg cgcatattct
     5821 cagagatcaa cttcactgtt ccaccagctc gataaatgat atcttccgtt ttcgccacga
     5881 tggtttccat cgcgatgctt ggcgtagtag tgatataaac caaatctcct tcttcaatct
     5941 tgacattacg gtgtgtaccg tttgccattt tttgcagaga cttgatgggt tcacccattc
     6001 ggccagtttc taaaatgatc agttcttctg gatcatagtt tttcatgtct ttcaacgtaa
     6061 caagcagatc ttcacttggt aattgaagtt tttctaaacg catcgctgtg cgaataattc
     6121 gttcgaaatc ttgaccagtc aagacaactt ttcgttgtgc tttttctgct gcatccaata
     6181 cttgttgcac tcgttgcaaa ttacttgcta cacaagcgac gatgatacgc ccttcccaat
     6241 atttgatcgt atcaaatact tcatctgcta tttgggcttc agaagctaca ggctgaggat
     6301 tttctgcatt tgccgattca cttagtaagg ctaagactcc ttctttgccg atttccgcca
     6361 atcgtgcata atctgtttga tacattggga tagccgtttg gtcaaattta aaatctcccg
     6421 tataaacgat actaccttct tttgttttca gattgatccc cactgaatct ggtatcgtat
     6481 gagtggtacg gaaaaagctc actactgttt caccaaagtc tatttctgta tgctcatcta
     6541 ccacatggaa atccttaaat cctttggctg cttcattacg actgacattc aattttgcta
     6601 actcgatcgt caactctgtc ccgaatactg gtacagaaat ttttgacaac aagtaaggaa
     6661 gtgccccgat cgcatctgca tgtccatgtg ttaaaaagac accagctaca cgatcaatgt
     6721 tttcttctaa atacgtaaaa tcagggatta cgacgtcgat ccccaacaac tcattttcag
     6781 gatattttag cccacaatct aaaataaaaa tatcctcgcc cacttctgcg acatacatgt
     6841 tttttccatt ttcacgaacg ccgcctaatg ggataatttt aatggtactc acaaacttca
     6901 cctcttctta tgacatccaa gtttaaagat gtcgattttc ttctttatct aaatatatga
     6961 gccattccaa tgaagcatat gcaaaatcac tatttctcaa cttatggaga atcagcagga
     7021 aaattaccag cacatccgcg ttctgacagt tggatgggca ttttattttg gatatacttc
     7081 attaacaaaa tggatctttt gcgaaacgct atagccagaa tacttatata agtattcagc
     7141 tgttccccat tattatacaa caaataaact aaatcgctag tatcagcaat tgttagaaca
     7201 actatgcacg taaaaatccc tctgtagcat ctaggatttc ttttgctatt cctctttcat
     7261 ttcttcttcg tcggtttgaa acatactttt ttcattttac cacatttttt gcaataacta
     7321 tagtgacttt ttatatatac agaacctcat gttgatggtg tgcaaacagc ataagattta
     7381 agctagcttt aagataggtt gtctattatc aaatgcatct actaggaggg atgcaaaata
     7441 tgaattttat gaaacgtgca tggaaaagta caaaagtaaa atggggacgt tcattgttat
     7501 tgtttgccgt gttcacagcc attcttattt ttgtgctagc tggcctcacg attcgaagcg
     7561 ctgcagaaga agcagcgaat caagcacaaa aagaagttgg agcaacagtg actttaagtg
     7621 ccaatcgaga agcttcattt aaaaaacagg aacaaacttc ttcaagcggt tctaccactg
     7681 aagaacgtcc agatccaggc agtttttctc ttacaccagt ttccttaacg gacgcggaaa
     7741 aaatagctga attagataat gtaaagagtt attcctttga aagttccgcc tcagctatgg
     7801 caaaagatgg aataactgcc atcactagtt ctgattcaag tgaagacagt tcagaaacta
     7861 ctgatgcagg cggtgaacct gctggaggaa tgggcgggaa tccgcaaatg actcaagcag
     7921 actttcaaat cagtggtgtg tcagcaactg cacaaaatag taatttttct gatggaacag
     7981 caaaaatcac ggatggtgaa ggaatagatg acagcgacaa aggcacaaac aatgtagtca
     8041 ttgattctac tctagcagaa acaaatgacc tggcagtcgg agatactttc acaatcacga
     8101 gtactgaaga tgaagatacc acttatgaaa tgacgatcaa aggaatttat gaatccagcg
     8161 aaacaagcgg cagtatgggt atgaaattca acttcatgaa tccgtcgaat acactttaca
     8221 cttcttacac ttttgcaaat gaactaaatg ggacatctga cgataacacg atagattctg
     8281 ctatttacac tctatctgat ccagataaaa tggatgagtt tctcaaagaa gctgagaagc
     8341 tgatcgacac agatacattt agcttacaat caaatgattc catgtatcag tcaatgttgg
     8401 aacctttaaa caacgtggca agtttttcta aaaatgttgt tttactagtg gctgtagccg
     8461 gtattatcat acttactctg atcattatga ttaccattcg ggagcgtcgg catgagttag
     8521 gcgtcttgct atctttagga gaatccagaa gtaaaatcat tctccaattg tttaccgaag
     8581 tggctatttg tatgattctt gcattaggag ttgcttcgct tagcggaaac gtcgtcgcga
     8641 atgccgtggg acagcagcta cttgaccaac agacagaaac cacgacacaa aaccagcaag
     8701 ctcctggcgg tggacaaatg ccaggtaaca gacaagaaca aggcggtcaa cgcgatggtc
     8761 aacaaggaac cacgaataat ccatttgctg tttctgatca agtcagcgaa ttggaaatca
     8821 atgtcaaacc tgcacaattg ggactattag ccggagtagc atttggaatc agtctgtttt
     8881 ctgtcttttt agcttctata gggatattaa gattaaaccc aagaaaaatc ctattaaact
     8941 aaaggaggaa tttgatgtta cggacagaac aattgggatt ttggtatcaa gatccgcaag
     9001 atgcgcttta taaagatgta gaactgaatt tcgaagctgg aaaaatgtac gctattttag
     9061 gagcaagtgg ttctggaaaa acgacttttc tttcgcttat ttctggacta gatgcaccaa
     9121 aagaaggaaa ggtatactat caagatcgtt cactgaacaa aatcgggtta agaagttatc
     9181 gtaaaaatga tgtctcgatt attttccaag cttacaactt gcttccttat ttatcggctc
     9241 tagacaacgt catagcagca atggaaatca ctaagaccaa gcaagagaat tatcgacaag
     9301 ttgctttaga tcgactggct cgtgtaggaa ttgatgaaat attagcaaaa aaacaggtga
     9361 ccaaactttc gggaggtcag cagcagcgag tcgctatcgt tcgtgctatc tgctgtgaac
     9421 atcaactaat cgtagcggat gagcctactg gaaatctaga tgaaacaact tcacaagata
     9481 tcgtcaaact tttccaagaa atcgcccatg aacaaaatcg ctgtattatc atcgtgactc
     9541 atgagattga agtagcaaaa agctgtgatg aagtctttga actaaaaaat aaaaaatttc
     9601 aaaaaatcga agtttaaaaa acaggataat gatcaaataa aaaatccgaa ccatatctgt
     9661 ctcgcagata tggttcggat tttttatttg cttgattcaa ttagtaataa gtggttttta
     9721 ttttttttcg tcttttcgag aaaacttgtc tttgacttcg tcgcctaaat ttcgagcttt
     9781 ttctttgatg tcagcaccta gtgattgtgc tttcccttct gtttctttgc tcttgtcgtc
     9841 tgttgctttg ccgtatgctt cttttccttc accgactact ttatctttca tatctttaga
     9901 acgtcctttt aaatctgcca ttgtgcttcc tcctcttagt ttgtttattg atctaccttc
     9961 agggtaacat caacaacaga tacagacaaa cagaacgctc gttttctaaa aaagatagtt
    10021 agagctgttg attaaaaact tttttcgaaa gtcccccata ctttgacttc tttttcttgg
    10081 accattattt ctcctttagc gataactgta tcaatatcaa gcgtttcttt atccaagata
    10141 cacagatctg catcagcacc aatttcgatt tttccttttt tatccaattt caatatacgt
    10201 gctggattac tggttatagc tcgtaaggcg atttccaaag ggatactttc tttttgcaca
    10261 gcttctttga tccctactag caatgccttg gcacttccta ctcctaaccc taggaaatga
    10321 ttgttttcat caaaataagg caagcttcct tgtccatctg aggtcatcgt gaaattatct
    10381 aaagaaacat cttcctcgat caatcgtttc agtgctttgc tgaagcgaac ttcaccatct
    10441 gtcttctccc aaaaatcagg atcttcgcta gcagtgaagt caatcgtgcc accacgtttg
    10501 gcaaatgcca cgcaagcttc aaataattct ggtgtgcgat ttgcatgagt aggcaaaaat
    10561 tgtgtgatcg gaatttctgt ttcttctacg attcgtgtca atagctctag ctttcttttt
    10621 cctccgccta agtggacatt gatgatccca gcttttccgg ataacatgcc tcctacacga
    10681 gcatctgcta ctgcacgagc gaactcttca aaacttggct gagaagaacg gtgatcggat
    10741 actgcgattt ctccgattcc gataatttta tcaattgtca aaaagtcttt gatgatagaa
    10801 tctgttactg tttttaccgg caatcggtac gaaccttcgt atacataagt ggagattcct
    10861 tctgcttcca gacctttcgc tttgctgatc aacgcagtca tatctctacc ttctccgtct
    10921 gttcccaaac aaccgacaac tgtcgttaca ccagctgtag tcaattggct caatgtgact
    10981 tctggcgttc gattttgata accgccttct cctcctccac ctaaaatatg gaaatgacag
    11041 tcaatgaaac ccggggttag tacttttccc tcacctgaaa gttcttctac ttccactcct
    11101 tcgtagccgc cttctagctg ttcttctaca gccaaaatct tcccaccggc aatcagtaca
    11161 tccatttttc ctaagtaatc tggtgcaaac acttcaattt gtcgaatgat tttcatacat
    11221 ttttcctctt tctttaagta taactgatga taggatctta aaaaataatc caaacgatag
    11281 gatctttact cctttgattt taccgtaaaa tgacggttct tggagtcctt atctactgtt
    11341 tggattactt ttttatttac aggtctccag tactactgca gacctattgc aatggcaatc
    11401 atgatgaaaa tagttcctaa aacaaacagg aatccttgca tcttgatttg gaatcttgcc
    11461 cattttgccc attcgatccg tgcaacacct agcacaccaa tcaagctggc tgatacgggt
    11521 gtaaaagcgt ccataaaacc tgaaccaagc tgataagcaa gaactgctac ttgtctggat
    11581 acacctacta aatctgccag tggagccatg attggcatag tcaaagctgc ttgtcctgaa
    11641 tttgatgtaa caaccaagtt gaacaaactt tggaagatgt acatcgccca agcaccaatg
    11701 acagctggaa caccggtcaa cgccgtcccg atcccatgca aaatcgtatt caaagctgaa
    11761 ggaacagatg catcagatcc tcccaagaca agaagaatcc ctttggccat acctactacg
    11821 attgctgtac cagctaaatc agctgcacca gattggaaag aagaagcgat gtcattgact
    11881 ttcattccat ctaatttgaa aagaacagca atgattccag caactaatcc catcacaaag
    11941 aattgagaag cgatttctgg aatgtaatag cctttttgag tcacgcccca aacgatccag
    12001 acaaggacaa ctaacatctc taataagatc aatttatgcc caagcatgaa ttttttattt
    12061 tcatcagatg tctttttgat atgagaacgg aaataatcat ctgatttgta agtcaacgaa
    12121 ctttctggtt ttttacggat tttttctgca tatatcatca tataaccagc ggctaatgca
    12181 gtaacaacta cccacataat caatcggaat gtcgcaccag acaacacggg gattccagca
    12241 atcccttgag ccaccgctac gctaaacggg ctcatccaag aggtcgcatt tccgacttga
    12301 gatgcgacat aagtcacggt tactgctacg atcgagtcat atcctaacgc aatgacaaat
    12361 ggaaccatca ccattgagaa tggaattacc tcttctgcca tcccgaatgt agcgccaccg
    12421 aatgaaaaag caaagaataa tagcggtaaa gctaaacgct ccagtccctt agttttactg
    12481 ataaacgcat agatacctgc gtcgatcgct ccggtacgca taataatacc aaaagcacct
    12541 ccaacaacaa ggatcaatgc agcgatccct acagcagatc catacttatc tccagatact
    12601 agtccttcaa aaacaaaatt taagaaacca aagccgccaa aatcatctgt tccccatatt
    12661 ttggctgttt tatgcaattt tttagaagta tcatagatat tttcgccgta ctgatcgtaa
    12721 agcacatcat ctgtcaagga gatttcatct agtttttcct gcgtaagatt tttttctccg
    12781 tcagcaatga ctttttccaa tcctttttcc ggaacatcca gtttttcacg ttctgctgga
    12841 tgatcttgca gttcttctaa ttgatcaaac acatacgact tatccagctc gtaagcataa
    12901 cggaaggaat cttgacgtaa aacagtacga gtacttgttt cgccatttgc atctttgtat
    12961 tcaatatctt gcgtgctgaa ctttcctgct ggaacgacaa aagtcagtat ccatgcaaag
    13021 atgactacac caaaaataat gacatacgta tggggtgtcg ataatttagt gaagtcgcgt
    13081 ttcggttttc ctgaagaatt tgttttcttc aaatttattt cttcctttct tttttcagta
    13141 attttgatga ataaaaaatg gataatcaaa tgaattatac agtaaaaatg acatttccac
    13201 atgaataatt tattagaaaa tcactttttt ctattttgtt ttttttctcc tatgtatgat
    13261 tatttttttc tctttccatt tatattaagt attttatttc tctgtttttc cttaaaattc
    13321 tttttttatt tttagattag agaaaatctc gtcctgtttt tttcatttta caatcattga
    13381 aaaattgaat ttcattgcaa aaaaaataat aaacacttat aaatatccat tatctatcct
    13441 tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca attttgttat
    13501 atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt tgtaaagagt
    13561 tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc atatggattc
    13621 tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc ttataactca
    13681 tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa
    13741 atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca
    13801 ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa
    13861 ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc
    13921 tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat
    13981 cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa
    14041 aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg
    14101 tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc
    14161 tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga
    14221 tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc
    14281 tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc
    14341 aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat
    14401 ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc
    14461 ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat
    14521 acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta
    14581 gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc
    14641 ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg
    14701 gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg
    14761 gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa
    14821 ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat
    14881 ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat
    14941 aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat
    15001 tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata
    15061 gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc
    15121 gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt
    15181 ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata cagtacagca
    15241 aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta
    15301 gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc
    15361 atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt
    15421 ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc
    15481 atagtgaaca cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat
    15541 cttctggctt ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa
    15601 aattaaacca ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc
    15661 gttttccacg actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac
    15721 gcacaagctg ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca
    15781 gttaactttc tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc
    15841 caatttccga atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc
    15901 ctacgtcgta tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag
    15961 tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata
    16021 tctcaaatat tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt
    16081 ttcccatttc aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg
    16141 ttttaggatc aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa
    16201 cagcggaagc ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat
    16261 ctttcatcaa atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg
    16321 cttgaagcac ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga
    16381 cttgagtcgt ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt
    16441 tttccaattc gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta
    16501 cgatcaagat gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg
    16561 gtttatccac gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg
    16621 ttcctagccc agcggcagga atgactgctt ttttgacctt cacttgct
//
LOCUS       DAFCNJ010000056        15949 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000056 DAFCNJ010000000
VERSION     DAFCNJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.057"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(386..808)
                     /locus_tag="LNE35_002218"
     CDS             complement(386..808)
                     /locus_tag="LNE35_002218"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBK6911339.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(822..2228)
                     /gene="atpD"
                     /locus_tag="LNE35_002219"
     CDS             complement(822..2228)
                     /gene="atpD"
                     /locus_tag="LNE35_002219"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBK6911340.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2251..3153)
                     /locus_tag="LNE35_002220"
     CDS             complement(2251..3153)
                     /locus_tag="LNE35_002220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBK6911341.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3167..4723)
                     /locus_tag="LNE35_002221"
     CDS             complement(3167..4723)
                     /locus_tag="LNE35_002221"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBK6911342.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4746..5288)
                     /locus_tag="LNE35_002222"
     CDS             complement(4746..5288)
                     /locus_tag="LNE35_002222"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBK6911343.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5275..5799)
                     /gene="atpF"
                     /locus_tag="LNE35_002223"
     CDS             complement(5275..5799)
                     /gene="atpF"
                     /locus_tag="LNE35_002223"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBK6911344.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5890..6105)
                     /gene="atpE"
                     /locus_tag="LNE35_002224"
     CDS             complement(5890..6105)
                     /gene="atpE"
                     /locus_tag="LNE35_002224"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBK6911345.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6158..6877)
                     /gene="atpB"
                     /locus_tag="LNE35_002225"
     CDS             complement(6158..6877)
                     /gene="atpB"
                     /locus_tag="LNE35_002225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBK6911346.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7179..10022)
                     /gene="rnr"
                     /locus_tag="LNE35_002226"
                     /pseudo
     CDS             complement(7179..10022)
                     /gene="rnr"
                     /locus_tag="LNE35_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10025..10780)
                     /locus_tag="LNE35_002227"
     CDS             complement(10025..10780)
                     /locus_tag="LNE35_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6911347.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10883..11119)
                     /gene="secG"
                     /locus_tag="LNE35_002228"
     CDS             complement(10883..11119)
                     /gene="secG"
                     /locus_tag="LNE35_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBK6911348.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11237..11515)
                     /locus_tag="LNE35_002229"
     CDS             complement(11237..11515)
                     /locus_tag="LNE35_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK6911349.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11628..12596)
                     /locus_tag="LNE35_002230"
     CDS             complement(11628..12596)
                     /locus_tag="LNE35_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6911350.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12811..12855
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12879..13853
                     /locus_tag="LNE35_002231"
     CDS             12879..13853
                     /locus_tag="LNE35_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBK6911351.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13858..14433
                     /locus_tag="LNE35_002232"
     CDS             13858..14433
                     /locus_tag="LNE35_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6911352.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14500..15639)
                     /locus_tag="LNE35_002233"
     CDS             complement(14500..15639)
                     /locus_tag="LNE35_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBK6911353.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3442 c         2856 g         5294 t
ORIGIN      
        1 tgagccagaa tgtggttcat tctgactcag atttgatttt gcacaactca tcagtcctta
       61 ttgacaaaga gcccaaaagt cttgtcgcag tcccttattc caaataaacg gtgttcaatc
      121 atctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttccatttgt
      181 ttctctaata ctcaggacaa aacgctgatt tcacaacctc cgatatacgg tgttccagaa
      241 gtagcttctt cgaaaaataa gctgagattt cacaaaaatt tgagaagcaa ttttcgtaaa
      301 ttccttctta tgtcttgaag ctgaccactt ctgtcataac ctctttttac tgttcttaaa
      361 cacggataaa catccgcatc tcattttatg agtgcttaga tactttgata cgattgattg
      421 ctcggtgcaa cgcaacagtt gcccgtttca attcgttcgt atcttgtttt gcttttgctt
      481 cttcgatttg gcgttctgcg cgttgttttg cacgttccgc acgagataca tcgatatcac
      541 gttcacgttc cgcactatca gcaacgattg aaacaacatt atcacgaact tccatgattc
      601 cgccattaac agcgatccaa tcgacgtggg tatctgaatc tgttcgtttt acacgaactt
      661 catcaatcgt caatggaacg atgattggtg catgtttagg caaaatccca agctcaccag
      721 ctgtcgtacg tgcaacgacg atagctgcat ggtgatcata gaccaaaccg gcaggagtta
      781 ccacatttac tgttaaatat tgatccatgg ggcacccctt tctagtagcc tagtgttttc
      841 gctttttcta cgacgtcttc gattcggcca acacttcgga aagcttcttc aggaagatca
      901 tcgtatttgc cttccaagat ttcgcggaat cctttcactg tttcttctac agggacatat
      961 gaacctggaa gaccagtaaa ttgttcagcc acgttaaagt tttgtgacaa gaagaattgg
     1021 actctgcggg cacgagaaac taacactttt tcttgatcgg ataattcatc catacctaag
     1081 atagcaatga tatcttgtaa ttcacgatat cgttgcaata cgtgttggac ttctgttgct
     1141 actttataat gttcttctcc cacgatttca ggtgctaaag cacttgaaga agaagccaat
     1201 ggatctaccg ctggataaat cccttgttct gttaatttac gttccaagtt ggttgtcgca
     1261 tccaaatggg cgaatgctgt tgccggcgcc gggtcagtgt agtcatcggc tggcacatag
     1321 attgcttgga tcgatgtgat cgaacctttt ttcgtagatg tgatacgttc ttgcaattga
     1381 cccatttctg ttgccaatgt tggttgataa ccaaccgcag aaggcatccg tcctaacaag
     1441 gctgatactt cagatccagc ttgagtgaaa cggaaaatgt tgtcgataaa caacagtaca
     1501 tcttgacctt ctacatcacg gaaatattcc gcaatcgtca aaccagtcaa agcaacacgc
     1561 atacgtgcac ctggtggttc gttcatttgt ccgaacacca tggctgtttt ttcgataact
     1621 cctgaatctt tcatttcata atacaagtca ttaccttcac gagtacgttc acctacaccg
     1681 gtaaatacag aaataccacc atgttcttgg gcgatattat ggatcagttc ttggatcagt
     1741 acggttttac caacaccggc accaccgaat agtccgactt tcccaccttt taaatatggg
     1801 gcaagcaaat cgataacttt gatccctgtt tccaaaattt ctgtacttgt acttaattcg
     1861 tcaaaggctg gtgctttttt atgaatttcg cttctttccg catcttctgg gaaaggtgtt
     1921 tctaaatcga tcgtatctcc tagtacgttg aatacgcgac ctaatgtttc tttacctaca
     1981 ggaacagaga ttgctttgcc tgtatcaatg acttccattc ctctttgtaa accatctgtt
     2041 gattccatcg caatggtccg gatcactccg tcccctaatt ctaacgctgc ttcaagaaca
     2101 acttttgatt tattttcgtc atttttataa acaactaatg cgttgttgat atctggtaag
     2161 gattggtcta aagaaaattc cacgtcgaca acgggaccga ttacttgaac aatcttgcct
     2221 gaactcatct tttttcctcc aatctcaata ctattctagt gccgaagctc cggcaacaat
     2281 ttcggtaatt tcttgggtaa tagccccttg tcttgcacgg ttgtaagaaa ccgtcagctc
     2341 atcaatgata ttagcagcat tatcagttgc tgttttcatt gctgtcatac cagcagcatg
     2401 ttctgctgtt ttggcgtcaa cgatcgcacc gtagatcaaa ctttcagcat actgaggcaa
     2461 taactgagac aagatttctt cttttgaagg ttcaaaaata tattcttgtt cgaaagtagt
     2521 tgcttcttct ggatctaagt ctgagattgg cagcattttt tctacacgaa actgactagt
     2581 tagcgaattg atatgatggt tataacatac gtataattca tcgaatactt cgttttggta
     2641 catagttgtt gccatattta cgattttgcg gacttcatca aagcttggct gatcagatag
     2701 attgcgcagc tcatatgcta gattgatacc gcgtgcttta aagaagtctg caccagttcc
     2761 gccaattgcg atcatcacgt aatcatcggg tgattggtgg tcttcttcta aaatagacat
     2821 cgtttgtttc aatatcgagc tgttatatcc accgactaaa ccgccatctg cagtgatgac
     2881 gatataaccc gttttcttaa caggacggct gagcaacatg ctgttgtaat tgatatcccc
     2941 tctaggattt tctgaggcaa tatcatttaa ctgagtagca gtcaaatgcg taacgatttc
     3001 tcttactttt gttgcataaa cctgaaactt ctgtgaagaa gcttcagatt ttgtcaactt
     3061 agaagcagac accatctgca ttgcccgcgt gatttggctc gtttttttcg ttgaagcgat
     3121 acgtgtcttg atttcattta atgaagcacc cattcatctc acctcactat gcattttgaa
     3181 tggatttcaa tttatcttct gtagaggaat tatttccttt tgtagctaag aacatgtctt
     3241 tatattcctg aatcgcactg ttcaacgctt cttcttctgg aagatctttt gttgtacgga
     3301 ttgtttcaaa gatatctgaa tgattggtat ccagatattc gaataattca tgttcgaagt
     3361 ctaaaatact gtctacaggg atactatcta agaatccatg tgtcaaagca tacaaaatca
     3421 ctacttgttt ttcaacagct agcggtgcat gcaatttttg tttcaagatt tctactgtac
     3481 ggcgtccacg atttagtttt gcttgtgttg ctgcatccaa gtcagaaccg aattgtgtaa
     3541 atgcttctaa ttcacggtaa cttgctaagt ctagacgtaa agtcccagca acttttttca
     3601 ttgctttgat ttgcgcagaa ccccctacac gagatacaga tagtccggca tcaaccgctg
     3661 gacgagtacc tgcgtagaac aagtcgcttt ctaagaagat ttgtccatca gtgatcgaaa
     3721 taacgtttgt tgggatataa gcagagatat ctccagcttg tgtttcaaca aatggtaagg
     3781 cagtcattga accgccacct aaatcatcac ttaattttgc tgcacgttcc aataagcgtg
     3841 agtgtaagta gaaaacatcc cctggataag cttcacgacc tggtggacgg cgaagcagca
     3901 aggaaagttc acgataagca acagcttgtt ttgaaagatc atcaaaaatg atcaagacat
     3961 gtttcccgtt gtacatgaac tcttcaccca ttgcagtacc agcatatggt gcgatataaa
     4021 gcaacggcgc tggttgagac gcaccggcat tcacaacgat tgtgtaatcc attgcgccat
     4081 attttttcaa tgtttcaact tgtgtacgaa ctgtagaatc tttttgtccg attgctacat
     4141 agatacagat catatcttga cctttttggt tgatgatcgt atcgatcgca atggaagttt
     4201 tccctgtttt acggtcaccg ataactaatt cacgttggcc gcgtccgatt ggtacaagag
     4261 cgtcgattgc ttttagacct gtttgcattg gttcgttaac agatttacgt tgcataacgc
     4321 ctggtgccat tgcttctact ggacgagctt tgtcagtaac gatttcacct agtccgtcaa
     4381 ttggctgacc tagcggatta actacccgtc caatcaaggc ctctccaact ggaacttcca
     4441 tgatctttcc tgttcgtttt actttatctc cttcacgaat ggactcgaaa tcgccaagga
     4501 taatgatacc tacatcattt gattctaagt tttgcgccat tccgtatgag ccatttgaaa
     4561 attcaagcaa ctcaccactc atcgcgtttt ctaaaccatg tgcacgagcg atcccgtcac
     4621 ctacataagt tacggtaccg atttcttcga ctgaaagcac gttttgataa ttttcaattt
     4681 gttctttaat caaggcacta atttcttctg ctttgatggc cattcgattc acctacctct
     4741 ttgtactatc tatttaattg attacgcata ttttctaatt gcgtgcgaat gcttccgtcg
     4801 attactcgat ggttagcttc aacaatcgcg ccaccaataa tagaagaatc aacgatttgt
     4861 tttaattcga ctgattgata gttcattgtt tttgcaacat tcttttctaa ttgtattcgt
     4921 tgttcgtctg aaagtggaac tgctgttgtg acacttccca acagcaaacc ttgatgttca
     4981 tcatatcgac gttcatattc ttcgatcatg aacaaaagat catccattcg gttatacgca
     5041 taaacaactt ctaaaaagtt tttaacgatt ccatcatagc cactaacaag cttgtccatg
     5101 atttctcgtt tttcatgagg ttctagacga acatcactta acatattacc taatccaggt
     5161 acttgttcat agatttgacg taagcttacg agatcttgat aaatttcttc tgctttattt
     5221 gaatcgatcg ccagttcaaa taaagctttc ccgtaacgtc ttcctactgt atatttatct
     5281 agtttcattt gaagaaccta gaccttcgat atattgattg attaatgatt cgtgcatttc
     5341 tggagataat tctttgttca agattttttc cgcgatttga agagatagtt ctgcaacatc
     5401 gtcttttaca gagttcagcg ctgaatcacg ttctacagtg atatcagctt gggctttact
     5461 ttttagacga gctgcttctt cttgagcatc cttcaaaata ttttggcggc ttaattctcc
     5521 actttctttc gcatttttga tgatatcagc tgcatcagaa cgagaggcta acaattgttg
     5581 ttcgcgttct tgttccattt tcgctgagtt gatgcgagat tgttctgcag aatccaaatc
     5641 attggcgatc ttgtcttcac gttttttcaa aatatcgctg attggtcccc aagcaaaatg
     5701 cttcaagaga gccagtagta tcaaaaatga gccgctgaca acgatgacat ttcctaacat
     5761 cgcattgcca acttctgcaa ttgccaattg attcagcata ggcgagcctt cctttctttg
     5821 aagtacttct agttttcatg taaaaaagca gtaacaaacc aaattgtttc tgccttaata
     5881 ctgattcaat taaacaccaa aaactagaat caaggcgaca actaccccta agataggaac
     5941 agcttcgact agagctaccc cgataaacat tgttgtacgg aattgaccag acatttcagg
     6001 ttggcgagcc attgattcga ttgttttaga gataacttga ccgttaccat aaccggctcc
     6061 gattgctgct cccatgattg cgattgctgc tgcgatataa ttcataattg ttttcctcct
     6121 aaattagata aatagtagtt tgtttttaaa acgaatatta ttcttccact tcaatcttat
     6181 ggctcatata aaccatagat aaagtaacga agacaaatgc ttggatgctt ccgataaaca
     6241 aggagaatgc gatccagacc atctctaatg gaattgctaa cggcaatgag aaccagccta
     6301 agcttattac catttttgca atcaaggtta gcaaaacttc ccccgcataa atattcccat
     6361 aaagacgtaa agcaagtgtt aataagtttg taaattcttc aatcagtttc atcggaaaca
     6421 taaaactata tggtttcaag aacgaattta cgaaatatcc tttgaagcct aactttttca
     6481 ccccaaataa gtgagtaagt aagatcatta taaatgccaa agttaaagta acaaacgggt
     6541 ctgctgtcgg gcttttccaa agtgttgttt cgttaggtaa aacgattttt gttactaaac
     6601 cgatgttgtt tgcaacaaaa acaaataaaa acaatgtaaa tgccaacaaa tggaaattgc
     6661 tcaattcttt tctaggcaga ttatctgtta cgattcctcg actaaaatca atgacccatt
     6721 ctatcgcatt ttgtttgcct gttggtttca acttaagatt tcttgtaaaa aagtaagcta
     6781 agaagaaaac aatcaaacag gtcagtaaca ccattataca aactgtacca tcaaaccaaa
     6841 ttggtccaat gtggaacgtt agcgagcgtt catccaatcc atttcacctc ttttccccga
     6901 aattcacata aaatcaattc atgtgcgtat ctggtttttt ccaatcggtt tctaaccaca
     6961 cggtatcata ccaccatgaa acagaaaatt caaaacattt tcatgaaaat tagacacttt
     7021 tttatttcag tcaaaaacga agcgaatttt tctttataca cggtgctcct gatctaagaa
     7081 aaaacctatg ataatcatgt tttatcagat taaatgctaa caaattttat cattaagtaa
     7141 aagcaaaagc accgaatatg cgaaaaagga taatgaaatt atctagagaa attttttaat
     7201 gttcgatcaa tttgtcgatc gatatctttt cgtttcaaat cttcccgttt atcataagat
     7261 tttttccctt tcgccagacc aatcaatacc ttggcatagc cgttacgaat atagactttt
     7321 aaaggaacaa cagtgattcc agtatttttc aattcatttt caagccggat gatttgtttt
     7381 ttgtgtaata ataactttct cgtgcgcaac ggatcatgat taaaaatatt tccttgttca
     7441 taaggactga tatgaacatt atgcaagaaa gcttctccgt tgcggacgcg aataaatcca
     7501 tcttttagat tgatccggct gtttcgtatc gacttgatct cggttccttg caagaccatc
     7561 cctgcttcca tcgtgtcgat gatcgaataa tcatggcgag ctttcttgtt ttgtgcaatt
     7621 aattttccct cgccttttgg catagctgtt tcctccaact cattttattt tttctttttc
     7681 ttctttttaa cacctttgta aaatggcttc ttcccttttt tcttaccgaa cgtttgctta
     7741 ctatcgctat atcgtttgtt cttcttgcca tttctttctg ggcgttttcc tgcttgtttt
     7801 ccttcgcgtt ttcctgattt tccatttcgt gtttttggcc gttccaacgc tgtaacctct
     7861 tctgcggaaa caagttcaaa atctactgct cgttcatctg gatcagcttt gactactttt
     7921 actcgcactt tctgtccgat tttaaaagtc agaccagttc tttcacctac aagagctaaa
     7981 tgattttcaa caaaatggaa gtagtcctgt ttcaattcat tgatatggat caatccctct
     8041 actgtattcg gcagttcgat aaacaggccg aatttcacaa cagagctgat gatcccatcg
     8101 aattcttccc ccactttgtc catcatgaat tctgctttct tcatggaatc tacttcacgt
     8161 tctgtttcta ctgcacgacg ttccatctta gaactatgat ctgcaatttc aggaagttcc
     8221 gtagcccacg aactttgatt gctttcactt ttatcttcac tgtaagaacg gatcagacga
     8281 tggacgatta gatcgggata gcgtcgaata ggagatgtaa agtgtgtgta gtaatctgcc
     8341 gctaagccat aatgcccaaa attgtcttct gaatagcgtg cttgttgcat gcttcttagc
     8401 aacatagtat tgataactgc agcttctggt ttgtcttcta ctccttcgat cacatgctgt
     8461 aaatcttttg gtgtgatcgt tcctttcgtt cctcggacca gaatgcccaa agctgaagca
     8521 aaatcaaaga agcgctgcat tttttcttct tttggctgtt catggattcg gtaaataaac
     8581 ggcagattta atttattata atgttcagct accgtttcat tggccgcaag catgaaagat
     8641 tcgatcaagc gttcaccaat tcctctgctt cttagctgga tatcatttgg gtgtccttca
     8701 gcatcaacaa ggatttttgc ttcgcgatcc tcaaaagaaa tcgcgccacg tttgattcgc
     8761 atcgtttcaa ggattcgatg caattcaccc atttcttgga acataggtac gagctgagca
     8821 tagcgttgca tcgtttcttc gtcttgatct tctaagattt gattgacagc tgtgtaggtc
     8881 atccgttctg ttgtttggat cacacttggg aaaatatcat gagagactac ttgtccattt
     8941 ggatcaatct ccatttcgca actcatggtc agccttggaa catggggatt cagtgaacag
     9001 ataccattcg ataaacgttg cgggatcatc ggaatcactc gatcagtcaa atatacactt
     9061 gtccctcttt catatgcttc tctgtccaat tgactgcctt gtgtcacata gtaagaaaca
     9121 tctgcaatgt gtactccaag aaaataatta ccgttatcta attttttaac cgtaactgca
     9181 tcatctagat cttttgcatc ttctccatca atcgtaacaa tgagctgatt tcttaaatct
     9241 ctacgtccag ttatatcctt ttcgctgatt tgatctggaa cttcatctgc ttcagacaat
     9301 acatcatcag gaaatttcgt agggatacca ttagccacga caattgagag gatatccatg
     9361 ccagggtcat tcttatgacc aatcacttgc ttgattatcc cttctaaact agtcgcatac
     9421 cctttttcag ggtaatgggt cagttcgatc agcacaatac ttccgtcaac tggacgaact
     9481 ccctctgctg ctgcatatac tttaaattgg ttgagctttc tatctttcgg gataacaacg
     9541 ccgtaaagat ccgtttcttg tatttcttct tctgaataag caacgaattc ccctgctaac
     9601 tgagtagtcg cccggtctcg aatctcgact actttcccct ctgctccgcg atccatatct
     9661 gtatttgctc gcttcatgat atcgattgct acggtgtctc catccattgc aaaaccagtc
     9721 gcttcttttg ggatatagat gtcatcttct tcgggatcaa tcgttacaaa tccaaatcct
     9781 cgttcatttg ctcgaaatgt tccttcgatc aagattggtt ttaaaggaag tttcactttt
     9841 ccttttttat tgaaaactat actctgttct ctttccattt gagcaatcgt ttgtactagt
     9901 aatttaaaat catcgctttt ttggaaccct aaaccttcag cgatttcttc catagaaaaa
     9961 ctgcgtttgc gactgctttc catgaaaaag agaattttct cttttatcgt ttcttttgtc
    10021 atatttattc ctcattccaa ggtaattttt ctaaaaattc caagacgtct ttttctaatt
    10081 cgcgtcttgc tgtaccaact gtaatcacat gaccactttc aggataccat cgaagtgtca
    10141 ctcgctgacg gctcaacttg cgtgcagttt caaatacgcc attagggtcg atcatttcat
    10201 ctttcccagc ttgagctaaa aatatcggct gttggatatc tgacaaccta ctttctacga
    10261 ttgctgcctg atcttgtata tccattaatt gttgttcagc taacgagcgg taagcaactg
    10321 ctttttcatt gatttctttc tctgatttac ctgctgtctt cagcacttga cgtacgtatt
    10381 tttcaaagtt ttcaggcacg tttgttttta caggagacaa aggcgaacag aaaaaaccgc
    10441 cgccaatcac tgattcttct tctagtgctc gtacagcaaa gatgccgccc attgataatc
    10501 ctaatacggc aatatcagaa aactgctcag actgtaaaaa atggatggct tttttagtgt
    10561 ctgcccacca gctttctgct ttttcttgca aaatatcata tggctccatt gttccgtgtc
    10621 ctttaaaaag cggcgcataa actgtataat ctgctttttc caaaaatcgt gcgagcattc
    10681 gaacatcgtt tggacttcct gagtaagcgt gaagcagcaa aaccgctcgc tttccatgtt
    10741 ttgcataaag gggttttggt aattgtctca ctcgtctcac ctctttcgcc tcattttacc
    10801 atagtttctt tcataacttc ttcttaagcg ttctctttgc aataaaaaaa gccgcccgcc
    10861 gttttgcgag aggcttattc aactacttcg aagataaata tgctaggaca aataataaga
    10921 tcatccagat agctcccata actgcagttg atcgctgcat gacagcttca aagccgcgag
    10981 ctttttgttt gccgaatagt tgatccgcac cgccggtaaa tgcactcgct gcactatttt
    11041 gcttgcttgg ctgcatcata atcgcaatga ccataacaat cgataaaacg atcacgattc
    11101 ccaaaattaa attatacata tcgcagcctc cttcccaaaa gatatcgtct ctttaacttt
    11161 atcatagata aaagtccgtg acaactctat ataaatggaa taacagctga aagttcaaaa
    11221 ctttcagctg ttattcttat gactgacgta agacgtcttg atattctggt ttcttttcaa
    11281 actctttttt tgcataaggg cataaaggaa tgatcttttt cccgtctcgt cttgctaatt
    11341 ctactccagt atacactagc ttttcagcta acttttgacc acgatatgct ggatctacaa
    11401 atgtatggtc aatgatcatg atgtctgggc ccgcatctga ccatgtcatt tctccgatct
    11461 cttgttgttc atcattgaat aaagcaagac gatttttttc ttctttgatt tccatatgcc
    11521 gcactcctca aataactgat atttttttat tcctacctta ttttagcagt ctcctgatct
    11581 ataaacaatt atttatcctc gaattactta caaaaaagaa gagaaaatta attcatcgtt
    11641 attctttgtt caataaatga aaaaatcact gtccaatagg ttttcggatc tttcatcgat
    11701 gcttccaagt gttctgcttc tggaacaagc agcagtgctt tatcccctgc cgtagcattt
    11761 tgaatcgtgt acgctgcttc tgtagggaca aaatgatctc tttcaccatg aatgaccaat
    11821 aaaggcagat ggttgctgcc aagctgtctc gtagctgatg cttgctttaa ggagtaaccg
    11881 gcgtatttct gggcataatg attcgcatat ctcatgacca tttttttagg aaagacagtg
    11941 attttactta acatatatcg gaattctgca taaacagaaa catatccgct gtctaaaata
    12001 aatcctttga ccgctgatgg aagtttttca ccacttgcca tcatgatcgt agaagctccc
    12061 attgatccgc cgtgtaaaat aatggacgca ttcggctgtt catctaagat gagttggatc
    12121 caggcaatta aatctagccg atctagccat cccattccga tgatttctcc ttcactttct
    12181 ccgtgcgcac gtaaatcagg tacaagaacg ttgaatcctt tctctgcata tcttttacct
    12241 atatgagaca tgtctctttt ccctgtgctt cggtaatcat gtacgcaaat gacccattta
    12301 ttctgcttat ttggatggat gaacatctga ccagctagtt tcaacccgtc ataacttttg
    12361 attgttttgg gtgttccctg ctcccaaaag ctttcacact cttcttctaa tttttgtcgt
    12421 tttttttcta ctgacgtgac tgcagcaggg tccatcaaac gatgaccagt gtcttcatac
    12481 cagtcatatc ctctctgtac tcccgctctt actaatttct ttgcataaaa ataagaaaga
    12541 aaatggtaac tgatccctat aattatcaga ataagaatga aataaaaaat aaccattgta
    12601 cacccgccgc tttctgccaa actataatct aactattatc caatgtaccg atccatagga
    12661 ttctttattt tcggcacacg taaattccat ttctttttca ttgtatcatt tacttaaagt
    12721 tctcctcaat agatttactt gtaagagagc atttacattc gcaataaaag ccagtatact
    12781 aatttcgtgc attataatca agacgctcgt cttggactgg ttcggaaact tcccagaata
    12841 aaaaactaag tatctctaaa gaaaggagat tggaaataat gcgaaaagta attattgatt
    12901 gcgatccagg gatcgacgac actttagctt tatcattagc tgtcaaatcc cctgatatcg
    12961 aagtagttgc tattaccgta gtttgtggga atgtccctgc agatataggt acgcagaatg
    13021 tgctgaaatg cctggaacgc tgtgatcgtt tagacattcc tgtttatcaa ggaatggaaa
    13081 aacctttgaa gcaacctttc atcagtgcac aagacactca tggtatggat ggtttagggg
    13141 aaacaaattt cccgatgatc ttgagaaaac aggcagaacc tctccatgcc gttgattttt
    13201 tggctgatta tttcaaagaa aagacaaata catcagtcat tgctttgggt cctttgacca
    13261 atatcgcttt tgctttgaaa gtcaacccaa atattgggaa acatatggat cgttttgtct
    13321 cgatgggtgg aacttacaaa agccatggta actgttcgcc agttgctgaa tacaattact
    13381 ggtgtgatcc tgaagcagct tcttatgtat tcgagaatct caaacaaacg atagaaatgg
    13441 ttgggttaga tgtgacaaga gaaatcgtct tgacaccgac tatcctcgaa tactgctgtc
    13501 aaatgaatcc tgaagaggga gagtatctaa aagcgatcac tcgtttttat tttgacttcc
    13561 actggaaaca agaaagaatt ttaggatgtg tcatcaatga tccgttagct gttgcttatt
    13621 ttatcgaggg ggccatctgt gaaggtttca aaagcttcac tgctgtggaa actcaaggaa
    13681 taagtcgtgg acaaacattg gttgatcgtt atgaattttg gcagaaacct gctaacagta
    13741 aaattatgac aaaagtagat actcgtcttt ttttccgaaa gtttttaacc gttcttttaa
    13801 atgcacaaga agaaacgatt atgaaagatt tggaaaggtt aaagatgggc taacaaaatg
    13861 aataaaaaca aaatggcaac tcgcgtactt acagtgatgg ctttgtgtat tgggatcaat
    13921 tatattggag gaacgattgc tttatggctt cgtttgccaa tctatctcga ttctatcgga
    13981 acgatttttg ctggtgcatt gcttgggcct gtacctggaa tgctgacagg attgtccagc
    14041 ggtattttaa gtggagtgac tacagatatt ttttctctgt attattcgcc aatccaaatt
    14101 ctcacaggct tgcttgctgg attgattttg ccaagatatc tcggcaaaga taaaaacagc
    14161 ttgaaactgt tattactcgc tttgattttg tcggtacctg gaacgatcgt ttcttcgatc
    14221 attaccgttc agttatttgg cgggatcacc tcctcaggat ccagtatgat cgttcagcta
    14281 ttacatgggt taggtttaag tcagacaaca agtgtgatct tggtacaggc aggtacagac
    14341 taccttgatc gtttgttgtc tatcgtcgtt gtttcattag tcatcactat gttgccacaa
    14401 cgcttgatat attcaaaaag aaaaataggc tgaataaaca gagctggatt agcaaaataa
    14461 tgctaatctg gctctgtttt ttcatttatt aaaaaaatct tacgctaagt aggataaaat
    14521 aattccttga tatttttcaa tcatatccaa ataattatcg atttcaacat actcatctac
    14581 ttgatgaggc aaagtaacga caccaggccc aaagacgaca aagtcgaagg aatggttagc
    14641 tttcgtaaat tcggctgcat ccgttgttgc tgccgctcca actaaaggta atggctggct
    14701 gaattgttgc tgaatgcaat gaatcaaagg agaatctgga tcagctttga caggtatttt
    14761 gttgtaatcg atcatcaatt ccaaatgata gtctgtctct tgattcaatt cattgacgat
    14821 tgattgcagc aatgcaatga ttttatcatt tgggtattct ggaatcgatc gaatgttccc
    14881 ttggagctgc gcatggcttg ggatgctgtt gacttgattc ccgccactga tcaatgtaac
    14941 attatggatt gtttttccta ggacaggatt ctcgattgtt tcagcgaggt gattcatttc
    15001 agcatttgct ttcgtaatga attcatttag gtgattgatt gcattataac cttggtctgg
    15061 catagagcta tgcgcttctt ttccgtgcga ggtgactgta tagttgattg atcccatgtg
    15121 cgtatacatc aaactgtaat tagttggctc tccgatgatc aaggcatcta agtcatctac
    15181 atagcctgct ttcgtgagct gttccccacc tagttcaccc acttcttctc cgacagtggc
    15241 taataatttg actgttccgt taaatggttt tcctgactct ttcagttcga tcatggcaat
    15301 caccattgct gccaagccac tcttcatatc tgtagcacca cgtccgtaca aacggttccc
    15361 atggatttct gctgcaaagg gtgcataggt ccaagacgat tcatcaccag ccgccacgac
    15421 atccatatgc ccagataggc ctaacacttt tcctgattgg cccttttggt aacgagcaat
    15481 caaattatct ctaccatctc tgtaagaaac aatctctcct gttatgtcat gctttgccaa
    15541 aagcttattc aaataggctg ctacttcacc ttcgtttcca ttcactgatt taattcggat
    15601 gatctcttgt aaaatcgcga ttttttcttc ttttttcatc taatcctctc ccttgccatt
    15661 tctttttatt gtatttgagt gtgctcgaat tttcaaatag gagcaaccaa agtaaaaatg
    15721 aagaaagaga gccactttat gaaaaatatg ctgctacttc cctcctattt tatttatacg
    15781 agaggacttg ctcatagatt cctattctac tgttctatcc ttaaaagctc ttatatgatg
    15841 ctagttattg ttagctttcc tttttcaagg atgatctttt cttgtaccat tctttgtaaa
    15901 catcatatac agaagctagt tgcaaaaaaa actctctacc tgctgatat
//
LOCUS       DAFCNJ010000057        15639 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000057 DAFCNJ010000000
VERSION     DAFCNJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.058"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            222..572
                     /locus_tag="LNE35_002234"
     CDS             222..572
                     /locus_tag="LNE35_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBK6911354.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            671..1201
                     /locus_tag="LNE35_002235"
     CDS             671..1201
                     /locus_tag="LNE35_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK6911355.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1355..2506
                     /locus_tag="LNE35_002236"
     CDS             1355..2506
                     /locus_tag="LNE35_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBK6911356.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2615..4654)
                     /locus_tag="LNE35_002237"
     CDS             complement(2615..4654)
                     /locus_tag="LNE35_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBK6911357.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4712..5269)
                     /locus_tag="LNE35_002238"
                     /pseudo
     CDS             complement(4712..5269)
                     /locus_tag="LNE35_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(5808..6014)
                     /locus_tag="LNE35_002239"
     CDS             complement(5808..6014)
                     /locus_tag="LNE35_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911358.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(6030..6644)
                     /locus_tag="LNE35_002240"
     CDS             complement(6030..6644)
                     /locus_tag="LNE35_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBK6911359.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6791..7474)
                     /locus_tag="LNE35_002241"
     CDS             complement(6791..7474)
                     /locus_tag="LNE35_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK6911360.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7583..8041)
                     /locus_tag="LNE35_002242"
     CDS             complement(7583..8041)
                     /locus_tag="LNE35_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBK6911361.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(8125..9888)
                     /locus_tag="LNE35_002243"
     CDS             complement(8125..9888)
                     /locus_tag="LNE35_002243"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBK6911362.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(10109..11569)
                     /locus_tag="LNE35_002244"
     CDS             complement(10109..11569)
                     /locus_tag="LNE35_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911363.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11777..12703)
                     /gene="glsA"
                     /locus_tag="LNE35_002245"
     CDS             complement(11777..12703)
                     /gene="glsA"
                     /locus_tag="LNE35_002245"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBK6911364.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12726..14153)
                     /locus_tag="LNE35_002246"
     CDS             complement(12726..14153)
                     /locus_tag="LNE35_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6911365.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14184..15398)
                     /locus_tag="LNE35_002247"
     CDS             complement(14184..15398)
                     /locus_tag="LNE35_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBK6911366.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4480 a         3117 c         2688 g         5354 t
ORIGIN      
        1 actcctttgg taggatgacg ttttggtcga ctaaatttta ccaaaagtga gtctcttttt
       61 gagttgaaac gaagaaaaag tgttagtaga aaatcgcctt cgattttcca ccaacacaaa
      121 atattataga acctttgttt ttttgaataa tcgctttttc ttctcttttt cttatatact
      181 ataagtagta aataatttga aaaaaagaaa ggtgactgat tatggcgcaa aatcgtattt
      241 ttacgacaag ttttgcaagc gtttatccta tgtatgtagc aaaagcggag agaaaagatc
      301 gtataaaaga ggaagtagat gaaatcattt gttggctgac gggttattcc caaacagaac
      361 tagaatatca actacaaaaa caagtgactt tcgaacaatt ttttgcggaa gcccctgtcc
      421 ccaatccttc acgttccttg attacaggtg ttgtttgtgg tgtacgagta gaagacatcg
      481 aggataactt gatgaaagaa attcgttatt tagataaaat gatcgatgag ctagcaaaag
      541 gcaaaaaaat ggaaaagatc ctcagaagtt gatttttcca gagatattct tctccttcgc
      601 tcattttgta acatttaatt tggtaaaatg actagttcac gttaaaataa gtcaaaggag
      661 gttttcttat atgtttgaga cgattaaaca aaatattcaa aaatacgcct tattaagagc
      721 tgctgtttat attatttctg ggattgcgat tgtgctaaac ccttctgctg tttttcattt
      781 tatcggttat ctgatcaccg cctatcttgt tttgcttggg ttgttgaatc tttttgaggc
      841 ttaccgtttt aaaaggaaaa caggttcttg gagtatcggt ctgttcagtg gtcttttttt
      901 cctgatctta gctctgatcg tctatgcttt tgcacctgca atcgtttcta tcctgccgat
      961 tcttcttgga ttagctgtta tcatcaacag cttgttccag ttgttcttca gcatgaatac
     1021 aaaagctaaa agttggagtg tttatagtag tattctgttg ttgattggcg gattcgtctt
     1081 attgttcaat ccattcaaaa gtttgatgct tctattccaa atattcggct ttattttgat
     1141 tttcatgggc atccaggaga ttatcaattt catcagaata agaaaaatga ctatacgtta
     1201 atcattttta gttattaaaa tagaccgtat aaaaaccaac aatccaaaaa aatcttttgg
     1261 attgttggtt tttttgtgag tatattttcc tattttttca ataatgtatg ttatgctaag
     1321 ttcgggctat attttataca aaggaatgat gagaatggaa tttattggta ttttatgcct
     1381 gatattgatt tcaacaacga ttggctcaca tatctcgcgg agattaggta tcccagcagt
     1441 gattggacaa ttacttgttg gtgttttgct aggccaagct ggattgaatt gggtcaaacc
     1501 tgatattcta gtccatgact tttcggagat tggtgtgatc ttattaatgt ttttagctgg
     1561 tctggaaagt gatttatctc ttttgaaaaa atatttccga cctggtatgt tcgttgcttt
     1621 gcttgggatc atcttcccgg ttgtttttgg ctggttgaca ggcaaagcat tccaagtttc
     1681 gggaaatgaa gcgattttct ttgggattat tcttgcggca acttctgtca gtatttcagt
     1741 agaagtatta aaagaactaa acgtagtgaa tacaaaagaa ggatcaacaa ttttgggtgc
     1801 ttctgtggta gatgacattt tagtcgtttt agttctaagt ttcagcctat cttttctaac
     1861 gggggaagct tctagcaatg cttctttacc gcttgttttg attgaacagc taatttattt
     1921 tgtttttatt ttcttattag tcaaatggat tgcgcctttc ctactttcat ttgcagaaaa
     1981 agtgtacgca aattcttcta ttatcatcgt atcgctagtt atttgtttag gaatgtctta
     2041 cgtagcggat cttgttgggc ttagctctgt cattggtgct ttttttgctg ggatagctgt
     2101 cagtcagaca aaggttaaag aagaagtata ccacaatgta gaggctttag gatatgcagt
     2161 atttatacca gtattttttg tcagtgtagg attagaagtt gatttttctg actttagtga
     2221 acaactactc tttatttcta tattgacgct tgtagcgatt ttgaccaaat tgcttggagg
     2281 ttattttggt gctcagattg ccggtttttc tcaacatagt tcattgatgg tcggtgctgg
     2341 tatgatttcg cgtggagaaa tggctttgat cattttacaa attggacagc aaagtcaatt
     2401 gatcgaacca cagtactatt cacctttagt tatcgtgatt ttattaagta cacttatttc
     2461 tccacttgtt ctcaaatatt ttacaaaaaa agtttatgaa gtgtgagtaa atcaaaaaga
     2521 agttgtgaaa gagacatcgt gaaaaccgtt tatttgaaaa aaggactgtg acgataacgc
     2581 cacagtcctt ttactgtact taaaaagcaa ttttttattg ataattttgg ttgaggtatt
     2641 gcaatagttc cgatgctcgt ttggttgctg aatcatcatc ttctttattt tccaacatgc
     2701 tgaccatcat ataaccttgg ttatcaagat tgaaagcaaa caagaaactg ttctcttgcc
     2761 cctttacatc ctgtttttct ttgatttccg ctgtaccagt tttcgctgcc aacggaatcc
     2821 ctaaagcaga aagagaatgt gctgtaccat ttacatcttg cacaacttct ctcagatctg
     2881 gcacgatcgt ttgtactgct gtttcgctga tgacattctt cttatctttt gtctctttat
     2941 ctgcaatcaa tttaggatag acaagtgtgc cattgttggt aaaaacagaa tacattgctg
     3001 cttgctggat aggagtaatt agaagttcgc cctgtccata tccagtatca gctagcaaga
     3061 tatctgagtt aaagctatct tcattagaaa tttgtgctgg attcatacta attggcaaat
     3121 caaggtcttc accaaaaatg aatttatcta agcctgtacg aaattttttc tcacccattt
     3181 tcaacgtttc ttgtgccgca tatatattat cggaatagat caaagcagtt tttaagtcta
     3241 cttgtgatac atcactactg acacgcgttg ttttaaaaga tccccaagaa ctgtcttttt
     3301 gccatttaag cccgttgatc gtcaacactt catttggatc gatagtgccg ttgtcgagac
     3361 cgattgctgc tgtaatcatt ttaaacgtag atccaggagc ataacctgtc gcaaatcggc
     3421 tgatgaatgg atgttcagga ttttcttcat aagctttgta atcttcttgt gagatcccgt
     3481 ttgtcatttt gtttggatca tagcttggag agctagcaag cgcaagaaga tcaccggttt
     3541 ttggcgttgt cgcaacagtt gatgcagctt ttcctcctag actgtcaaaa gctatttttt
     3601 gtgccttcgc atcgattgtc aatttgatat ctttgccatt ttgaacttca tgttcgatca
     3661 gaactttttt ctctacaccg tctgcatctg taatgcttaa tttcccacct gttgtcccac
     3721 gaagatcctt atcaaaagcc atctccaaac ctgagcgacc gatttttcca ttactgctta
     3781 attctggatt cttatcaata tcttctgctg tgatatctcc cacgtaacca atcagctgag
     3841 cagctgcttc acccaaagga taatatctgc cgtctacttc ttgaatggta gctccagctg
     3901 gaagttctgg cgttgctcca tcaatgattt tcaatgggac aaagtaatcg ggttgtaccc
     3961 agctttgtga aatcgcctga ttgatagcat cttctgttaa gtcgaaggag gaagcaatcg
     4021 ctttgatatt ggctgttttt tcgcctccat ctccaagttt acttggaacg actcctaatt
     4081 gttttagttt gcctgttgtt gctaatggtt caccatttcg atctataata ttccctcttg
     4141 ctgcttcttg tgtggtcagg cttactttgt catttccttc catttctgga aaaaccaagt
     4201 tgggttgcca gttgatcgtg gtttgaccat catttctgtc taatgttcct ttataagaaa
     4261 gatctttcaa ttcacctaat gaggtattca tctttgcttt atatgaaaaa ctgtaagtag
     4321 aatcgtcttt tttatctact tttagattcg atatctgaag tcctttgaca tcagcagcac
     4381 cgtatatatt ctggtattta tctaagatct ctttttcaga taatgcactt ttatttgccg
     4441 ccttttttga tgtcatttct gcagctttgt tataatctcc cttgttcaaa gcttggacaa
     4501 attgctcaac tgtcttttct ccagcttcta ctgcttgggt ttcttgatag tgctggtagt
     4561 aaaaataacc gccacccgca gctgctatta agatcactgc gccggcaata taagcgcctg
     4621 ttcgattttt gccgtgcttg tcacttcttt tcattttttc ctccttctct tgcttgtcat
     4681 tcctttattt cactatttat acctgtttat tctaacatat gtctttgagg aaatccatac
     4741 cacaatccag aagcacactt tctggaaggt tcgtttggat actaccaagt aatttcccaa
     4801 ccgctttggg tagcttgaat attgaagtta cgtctttcag ctgctgagag atagctttga
     4861 tcacttgctg ctgccttctg atcctcccga agtctccttc tttgtcttcc cgaaatcgag
     4921 catactggag taaagtatta ccatccatca cttgctctcc tttgtttata tatacgccat
     4981 ctaaatctag agatttttct gcatcgatct tgactccacc aggaaacagt tcattaacgc
     5041 aatcgataaa atcttgaaat gtgatacttg cataataggg agcttcaaat ttgaaatttt
     5101 cctttaatgt ttgatttaat aaatcaactc ccccataaga gtaagctgca tttatcttat
     5161 tcattccgta acctggaata tcgacatatg aatctctcat gatagagatc agttttgctt
     5221 gttgtgtttt ctggttgtat tgtgcaacca gaagtgaatc cgagcgacct ccaccaatcg
     5281 atcttttttc accgcatctc ctcctcacac agcaatccac ttgtcaggtg aggagcgagt
     5341 ttgtctcatc aaataagtca gtcacaaaaa tataaagaaa aggtcagaaa ccttttcgaa
     5401 caagaattct gacctagcat catctcatcg tccgagtttt agcactatac aacgtaaaga
     5461 aaacattctt agctattgga aaaagcctta atccaacagt ttctgtttta cccattggcg
     5521 actttcgacg tttctgggca acagcctatg tttgtatagg agcctcacct aacaaggtta
     5581 ttactatatt ctatttacga gttcatgata gacttttcct aatgattagg caatggaatt
     5641 tgaaatgaaa tagtgaaatt taacaatctt taatattttt tagaaaaaaa taacaaataa
     5701 gttgaaagaa tcccgttgca aattctttca gttgcaccag gattctttga taatttttta
     5761 gttttatgtg tttttttata tgttttttca cttttcggaa taaatattta tttgttaaag
     5821 taattaaaca atccgctaat cgtttgatct ggcatttcta tttccttacc atctaaagta
     5881 acagtagtaa cgacaaaatg gtcttgcaat ggcggaactt ctaagtactg cccttgcaga
     5941 aaatcattca ctgtttcata ttcttttttc atttcttttg aatctgtcga tggtttatag
     6001 cttattttta acataactag tgctcctttt tattcgggac gtgcaatcgt gaaaatttct
     6061 cctattgctg cataatcatt tcctgccaaa cggcttacag cattcaattt tcttggatcg
     6121 atccttcctg tcttatcatc atagactgtt tcatttaaat gatatttctg tacttctagc
     6181 aaaaataaat cagctgttac tttattttta ttccgtatct cgacatgttc atataaggat
     6241 gtttcaaaac gaacttttgc ttctgtcaat gctggtactg aaatgattac tgaatcagtt
     6301 aaagtcaagt cagtcaggta cagttcgctt tcctcattag gcagattggc tgctgtttta
     6361 tttacttctt ctacgttgtc ctcatcaagg atatggataa cagcttctct tgtctcaaga
     6421 atattctttg ctgtgtcttt tactgcatct tgttttctct ggatcgccaa ggataagatg
     6481 ggtcgattcg aactgacgat attgaagtaa ctgaagggtg cgatattcac tatatctttt
     6541 gctgatttag tcgttactac tgctactggt ctagggatga ctgaaccaat cagcaacttg
     6601 taattttcac gttctgtcaa ttctgaagga ctccgttcaa acatggtttc gccttctttc
     6661 tataaaattc gttaaaaagc agatcttgaa gacaaaatga aaagtaatgc ttttttcatc
     6721 ttcttagaat ctgctttaaa ctatctatca atttttcttt tttgtcaaga atctgtgccg
     6781 tgcgtgtttt ttagttattt gctttacttg cggcttcgac atatctttta gtgatcaatt
     6841 gcggacgggt gataatagat ccaactacga ctgaaaaagc acctaattca tatgctttct
     6901 ctacttgttc aggagagtca gtatgtcctt ccataattac tgggatatcg acttgttcta
     6961 tgatttgatt caataaatca aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc
     7021 ctgctaaagt cgtagacaaa agatcaaaac catcttcttg cgcttgaagc gcttcctcaa
     7081 aagtcgaaat atctgccata gctaaccgac cgtgttcgtg aatcatttgg acaagttcag
     7141 cagttgtttt ttctgtcgga cgtttacgag aagttgcatc cagtgcaatg atttctgctt
     7201 gcgtctccaa aagttcttcc acttcttttt ctgtcgcagt aataaacact tctgacccat
     7261 cgtaatctct tttgataatc cctattaccg gtaaatcaac gattcgcttg atttcattga
     7321 tatcttctgt cgtatttgct cgaataccaa ctgctccgcc ttcttttgca gccaaagcca
     7381 ttctccccat aataaaagaa ctgtgtagcg gctcgttttc caaagcttga caagaaacga
     7441 cgactccgcc tttgatttgt tccagtgtat ccacaatatc tcctccagta atgttatata
     7501 aaaatacagc ttcatataaa aatgtaaccc ttttctattc aggagacaat aatgagctat
     7561 ttctttcagc agacctttct tactaaaaga aaattacttc aaattcttcg tatacgacca
     7621 gcttctcttc tgtaaattct atgttcaacg cttctttata agcatgttta aaaaactcag
     7681 catggacttt ccgccaataa gctaatgtcc gatccccttc tccttctttc caagcatgct
     7741 ctttcgtgac ttcactaaaa ggcaagatgg tcacatttat atttttgatg atacaaactg
     7801 gttcattttt tccattcagg accacgtcta attgaccaac ttttggtaaa ggctcattct
     7861 cttgtttata cagatcgtaa ccacttgatg taccagtctt tatctttttt acaactagtt
     7921 cgctcaactc gtctgccatt ttttctgtat ccccaaatga aaagaaacct aatttttctg
     7981 attgataact aggatatttt tcttggaact ttttgacata ttcttctact tgctgataca
     8041 taacatccac ccctttgctt tttataatga aaaaaccatc cagatactag tataaacaat
     8101 tatctggatg gtgagcataa tgaattattg aatattcaag acacgcagac tatcttcaac
     8161 gatttgtttt ggtgttaaat gataacgttc ataaagctgc cctacaggaa cgctatccgt
     8221 gaattctttc tctgcaccaa aatttaatac gttcattgaa gaatcaccat aataacgact
     8281 gatattttcg ccaaatcctc ctgacaaact accatcttct aatgtgatga ctaattcgtg
     8341 tgtttctttt aatttatcta atgtttctac gtcattttct gtgatcgttc ctggattgat
     8401 caacgttgct tcgattcctt tttttctaaa agcttttact gtttcttttc ctaattcaag
     8461 aaaaccgcct aaagctaaaa ttgctacttt tcctccatta tttactgttt gataggctgg
     8521 aatactataa tcatttgcga tagctgtacc atgttctaca ccatgtgtag gaattcggat
     8581 tgctacaggc tgcgctgttt gttcgaacgc ccaatctagc atagagatca tatcctcttt
     8641 tgttgctgga gcaagatatt tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt
     8701 tccttggtgt gttcggtcac tagctgagat cgttccgcca ccgactaaaa taatcactgg
     8761 attctcgttg atcgctaaat catgtgataa ttgatcatat gcccgttgca agaaagtact
     8821 tgattggaag acgactggac gtactccctg agaagccaaa gcagaagcga aagtgataga
     8881 gtgctgttca gcgatcccca cgtcaaaata atgatctgga tgtttttctt ggaatgtttt
     8941 cagtccaaag gcgcctggta tcgctgcatt gactgcggca ataggagctg tacttgtttg
     9001 cattttctta tccaaataat ccatgatgat catattaaat gtttctccag aagttacttg
     9061 ctttgcctcc ccggtggaca gatcaaatgg tacatgccaa tgatatttca ttttattatc
     9121 gattgccggc tgataaccgc gacctttttc cgtatggacg tgaagtacaa tcggatgatc
     9181 aatatctttt acttcttcaa ataaacggat caccgtttcc agatcgtttc cattatccac
     9241 gtaacgataa tcaaatccca ttgctttaaa taggttattg ctgcttttac cgttggtttg
     9301 acgaagctca gctaagtttt tatataaact gccatgattc tcatcaatag acatctcatt
     9361 atcgtttact aatacgatta gattggattg taaaacacca ccattgttca atccttcaaa
     9421 agctaagcca ccacttaggg agccgtcacc gataaatgct accacatttc ctgattcttt
     9481 cttcagatct ctcgctttag ctaaacctgt tgctaagctg atagatgtag atgtgtgccc
     9541 cactgtaaaa tagtcatgtt tactttcatg ttggttggaa tatccgctca cttcacggta
     9601 atgtcctttt tcaaaaccat tttttcgacc agttaaaagc ttatgaccat aagactgatg
     9661 ggaaacatcc caaacgattt tatcctttgg tgaatcaaat acataatgaa acgcaatcgt
     9721 taattctact acacctagat tcggaccaac gtgcccacct acatcgctgt ccttttgcaa
     9781 gattttttgg cgcatctctt ctgccaactg tttcatttcc tcaacagata attttttgac
     9841 atctgcgggg ctattgattg catccaatac atgtaatact gtactcataa tacattctct
     9901 cctccgtact atcttctttt aatgaaaata gatttgttac actttatcta ttctactact
     9961 tagagttgac tctaagtcaa tagcatttta aaaaaatttc agagaaagct tgttatataa
    10021 gtagatcttc gcacgaatca ggactcaaat aaaaaccttc tacagacaat taaaaagctt
    10081 tgtagaaggt ctcctaatta aggtctcatc aagaaatatg tttttttaat aggtgaagtt
    10141 cctgagcaat atgatacaaa tggatgtttg caccttttaa tgagattgga tagatctttg
    10201 tcttttcagt ttctaatcca ttttgatagc tagcagcatc gcacaaaacc actctttttt
    10261 tcgcacatag acattcatca ggcaattctt ctaatgaacg aaataaaata ggatttattt
    10321 cagcgatgcc ttcttccgca tacaaatgaa attgggttcg ataaaaaaat tgtttgactg
    10381 aactttctga aacaacaaaa atttctattt cctgtctccc cgcttgttca ctgttgatca
    10441 aagaaagact tttgacgaat tttcgaatga aattttcatt ccatcttagt tggatttgat
    10501 ttttcttttt ccactcttcc aatatttcta caatttttcc gtacagaagt tgttcttttt
    10561 ctgatagaag atagatcctt tccggtaaaa atacttgtcc atctgcccac aacgtacgga
    10621 caaattgtaa aaaggattgc ttgaaaataa aattttctgc atcgtcgatt tcagcgatac
    10681 taataatctg taaaatcaat tcattcagtg ataggtcatt gagaacaaaa tttttataaa
    10741 caacttcgaa atcttttctt aacagctcca tgttctcatt ggtataattt ctactgttga
    10801 acaaagaaaa aatataatag acatcttctt cttgtaagtc tagaattcct ttttgctctt
    10861 ccaatccttg acagactaat tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt
    10921 ctttcagtcc aaggggaaat gcctttcttc cgattgcgat ctctattcct cgtatgataa
    10981 aggacttttc atcttccgaa tatcttcttt taaaaaaatg agtctctaca taatcaatca
    11041 actcgttaca agcttcttct acatacggat ttttattttc atagccttcc caattgacat
    11101 aacgtactaa agctagcaag atgttccgcg agtcaaattc tgggataatg atttctttat
    11161 ttttcaaata atcattttct ttaaaaaaat ccaatacgat ttttttgatt gtatatactt
    11221 tgctcaaaga aataaattca agatcagcca gttctgtact cgtgaatata ccattaaaat
    11281 aataacttaa aaactttaaa aaataagatt gaccgtacag aaactgtgtg agctcagaaa
    11341 gagcaatacc atgtttgaat acgagtttat acttatgctt ctctcttact tgttctttta
    11401 tacaatattc tagacactcg gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca
    11461 cccttagaga aatagctgtt tgttcgatgt ctatttcttg gaattcaaat aattgttcac
    11521 acagatacac ttttctaaat atatttcgct cgatgtaatt ttccagcatt cctgatacac
    11581 gcctccagtt tttcttatga ttttatttaa tcatatctgt aaatcgtatt tttgtttcct
    11641 ttttatttag aaaagccctt tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa
    11701 caagctcaca atttcagcct cattgtaggt agctttgagc cgtttggact tttaccccag
    11761 cctctttcaa gaatggttat tcaaagatgt cagcgtctag ttctttgact acatctttca
    11821 acaattggat accagctgcg ctgttaccaa atggatctag tgccgggctg aagacaccta
    11881 tgccgtaacg atttggcaca gctgccatca acccgccgcc tacaccgctt tttgcaggta
    11941 caccaacatg gacggaaaac tctcctgatt catcatacaa accagcagtt gtcataattg
    12001 atttgacgat tgtagcactt tcttcagtaa tgatttgttt gccattccaa ggagcaattc
    12061 ctttatttgc cagcacagca gctaattttg ctatgcctgt agcggtgaca ttgagggagc
    12121 attgtctgaa ataagcatcg agtatctccg gtacgtctcc ttcgatcatt tgattacctt
    12181 tcatatagta agcaagagag cgattgatat cacctgttct tgattcagaa tgatagattt
    12241 cttcattcaa tgtgatatta ggatcatcac agatctcttt cataaatgac aagattcgct
    12301 caaatttttc atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat
    12361 taacaaaagg attcattgga tgtttgcgat gattgatttc catattcatg atagaattga
    12421 aggcaaaacc ggttggttcc gtgttgattg tttgaaagac tttttctatt ccgttgtctt
    12481 tgattgctaa taacaatacg ggaacttttg acatgctttc gatggcaaag cgcacatcgg
    12541 cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata aatcgccata cctaattgtt
    12601 tgggatccac ttcagctaat gctggaatgt aatccgccac ttgtcctgtt tgataatagt
    12661 ggaaattttt tccaatcaat tgttctaatt cttttacttc catttgaata accagctttc
    12721 attttttatt tttctgcatg ctggaattta tgacggcatg cgtagatcgt aaatggaata
    12781 actaagacga taagataaga aacaatcaga atcgtttcat aaatcccatg ctgtccagct
    12841 gcgatactag caggtggaac aaatgagata caaaaagcaa aaagtgaagt caaaagacct
    12901 aaacaagcaa cgattacttt tacaactttt ccgcctggaa cctgataggc tctttcaaga
    12961 ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa acataaagta caacatcagg
    13021 tagattacaa cagtcaatga catcgctgca agaaatgaaa gattattccc cccgccgctt
    13081 aatgtaaaga cggctgccca gattgttgca acgatcccat ttgcgatcat caaatgagca
    13141 ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct
    13201 acgtgaagtg cttttgaagg agaggttacc caagaagcaa tttctccgat gacacctaaa
    13261 caaacaagaa tcgcaagaat tttcgcaatc catgcaagat ttggatcaat gtgtcttaat
    13321 aggaattcta acgattgaat cacccctgta ttaagagaca aaccatgttg aggtactgta
    13381 gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg ccagaataac caatacaaac
    13441 attgctaatg gataatcctt tttagggttt tccatttcat ttgcatgact ggcagatgtt
    13501 tctacgccca tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa
    13561 aaatcaggaa tcaatgtttt catagacagt atcgtttgta gtggagcgcc tgaagcgaca
    13621 taatggatcc ctaagccaaa caagactaat gctggcaaca agataccagc tacaaaaccg
    13681 atccgagcaa tcaatgaagt gtatttggtt ccttttaact gtgagagtaa aatagcccag
    13741 aaaataatca ataggattgc caatttcaga tatttattgt tttgtatagc accaataccc
    13801 gttgcatagg ataatgcacc agttaagaaa tacagcatgg tcaagtagcc aactgttatc
    13861 tcaaaccatt ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc
    13921 caagtataga caccgccttt ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat
    13981 gccacaggga taaaccatag caaaccgcct aataacaaga aaaatactaa gctgaagcct
    14041 gaggtcgcga atgtgggata ttcatataat gaaacgacca ttgagatggt catagccaaa
    14101 aagccaaaga acgttaattt ttttgcagga attttcgttt tgctttgttg catgaatctt
    14161 cctcttttct atttgaattt gtttcaattg agtttgttgc caaagaagaa aaagtccaat
    14221 ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa
    14281 atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat cacttgaatc
    14341 cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc aaagatccct
    14401 atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc aagtgcatca
    14461 tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc cgcaatgatc
    14521 ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat ggctgccaat
    14581 ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt tacttttgca
    14641 atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc aaatgcattg
    14701 actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca gccaaaccaa
    14761 agcagtcctg taccgatagc agctgccata agactgctat gctgacttct ttctcctaaa
    14821 ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc agctgtcgtg
    14881 tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa tccgccaccc
    14941 catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac aagtataata
    15001 tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg
    15061 ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg
    15121 ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc aataacaccg
    15181 ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat cgttgttatc
    15241 ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg tgctaatccg
    15301 ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca taaaatcatg
    15361 aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc ctttctacga
    15421 ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa ttaatacttg
    15481 catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc
    15541 acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt tattttcgtc
    15601 tttttttatt tttaaagata ttttttcata aggaataaa
//
LOCUS       DAFCNJ010000058        15230 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000058 DAFCNJ010000000
VERSION     DAFCNJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.060"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            278..787
                     /locus_tag="LNE35_002248"
     CDS             278..787
                     /locus_tag="LNE35_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6911367.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="LNE35_002249"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="LNE35_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6911368.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="LNE35_002250"
     CDS             2322..3302
                     /locus_tag="LNE35_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6911369.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="LNE35_002251"
     CDS             3424..4842
                     /locus_tag="LNE35_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBK6911370.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="LNE35_002252"
     CDS             4913..6382
                     /locus_tag="LNE35_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6911371.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="LNE35_002253"
     CDS             6392..6859
                     /locus_tag="LNE35_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6911372.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="LNE35_002254"
     CDS             6895..7767
                     /locus_tag="LNE35_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6911373.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="LNE35_002255"
     CDS             7848..10382
                     /locus_tag="LNE35_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6911374.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="LNE35_002256"
     CDS             10538..11311
                     /locus_tag="LNE35_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6911375.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="LNE35_002257"
     CDS             11304..12095
                     /locus_tag="LNE35_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6911376.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="LNE35_002258"
     CDS             12105..12596
                     /locus_tag="LNE35_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6911377.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="LNE35_002259"
     CDS             12614..13024
                     /locus_tag="LNE35_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6911378.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="LNE35_002260"
     CDS             13028..14428
                     /locus_tag="LNE35_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBK6911379.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="LNE35_002261"
     CDS             14451..14825
                     /locus_tag="LNE35_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6911380.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DAFCNJ010000059        14815 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000059 DAFCNJ010000000
VERSION     DAFCNJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.061"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(296..1024)
                     /locus_tag="LNE35_002262"
     CDS             complement(296..1024)
                     /locus_tag="LNE35_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBK6911381.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="LNE35_002263"
     CDS             complement(1002..2576)
                     /locus_tag="LNE35_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK6911382.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="LNE35_002264"
     CDS             2805..3743
                     /locus_tag="LNE35_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6911383.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="LNE35_002265"
     CDS             3755..4666
                     /locus_tag="LNE35_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6911384.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="LNE35_002266"
     CDS             4690..6162
                     /locus_tag="LNE35_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6911385.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="LNE35_002267"
     CDS             6213..6824
                     /locus_tag="LNE35_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK6911386.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="LNE35_002268"
     CDS             6854..8767
                     /locus_tag="LNE35_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HBK6911387.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="LNE35_002269"
     CDS             8895..11876
                     /locus_tag="LNE35_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HBK6911388.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="LNE35_002270"
     CDS             11873..13534
                     /locus_tag="LNE35_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HBK6911389.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="LNE35_002271"
     CDS             13574..14506
                     /locus_tag="LNE35_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBK6911390.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAFCNJ010000060        14482 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000060 DAFCNJ010000000
VERSION     DAFCNJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.062"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(287..649)
                     /locus_tag="LNE35_002272"
     CDS             complement(287..649)
                     /locus_tag="LNE35_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911391.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="LNE35_002273"
     CDS             complement(723..1103)
                     /locus_tag="LNE35_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBK6911392.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="LNE35_002274"
     CDS             1167..2360
                     /locus_tag="LNE35_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6911393.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="LNE35_002275"
     CDS             complement(2437..3021)
                     /locus_tag="LNE35_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6911394.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="LNE35_002276"
     CDS             3222..4220
                     /locus_tag="LNE35_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6911395.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="LNE35_002277"
     CDS             4332..4841
                     /locus_tag="LNE35_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBK6911396.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="LNE35_002278"
     CDS             complement(4871..5530)
                     /locus_tag="LNE35_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBK6911397.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="LNE35_002279"
     CDS             complement(5532..6296)
                     /locus_tag="LNE35_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBK6911398.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="LNE35_002280"
     CDS             complement(6304..6981)
                     /locus_tag="LNE35_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBK6911399.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="LNE35_002281"
     CDS             complement(7018..8403)
                     /locus_tag="LNE35_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK6911400.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="LNE35_002282"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LNE35_002282"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="LNE35_002283"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="LNE35_002283"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBK6911401.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="LNE35_002284"
     CDS             9342..10199
                     /locus_tag="LNE35_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6911402.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="LNE35_002285"
     CDS             complement(10280..10615)
                     /locus_tag="LNE35_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911403.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="LNE35_002286"
     CDS             complement(10682..12052)
                     /locus_tag="LNE35_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBK6911404.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="LNE35_002287"
     CDS             complement(12130..12666)
                     /locus_tag="LNE35_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6911405.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="LNE35_002288"
     CDS             12975..14456
                     /locus_tag="LNE35_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6911406.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4461 a         2883 c         2353 g         4785 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta tt
//
LOCUS       DAFCNJ010000061        13943 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000061 DAFCNJ010000000
VERSION     DAFCNJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.063"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            136..309
                     /locus_tag="LNE35_002289"
     CDS             136..309
                     /locus_tag="LNE35_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911407.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="LNE35_002290"
     CDS             949..1422
                     /locus_tag="LNE35_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBK6911408.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="LNE35_002291"
     CDS             1431..1658
                     /locus_tag="LNE35_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6911409.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="LNE35_002292"
     CDS             2294..2665
                     /locus_tag="LNE35_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911410.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="LNE35_002293"
     CDS             complement(2921..3163)
                     /locus_tag="LNE35_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6911411.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="LNE35_002294"
     CDS             complement(3195..4097)
                     /locus_tag="LNE35_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6911412.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="LNE35_002295"
     CDS             complement(4110..4298)
                     /locus_tag="LNE35_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK6911413.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="LNE35_002296"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="LNE35_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK6911414.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="LNE35_002297"
     CDS             complement(4913..5806)
                     /locus_tag="LNE35_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6911415.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="LNE35_002298"
     CDS             complement(5884..6807)
                     /locus_tag="LNE35_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK6911416.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="LNE35_002299"
     CDS             complement(6856..7206)
                     /locus_tag="LNE35_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6911417.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="LNE35_002300"
     CDS             complement(7239..8141)
                     /locus_tag="LNE35_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK6911418.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="LNE35_002301"
     CDS             complement(8134..8991)
                     /locus_tag="LNE35_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK6911419.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="LNE35_002302"
     CDS             9325..10134
                     /locus_tag="LNE35_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6911420.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="LNE35_002303"
     CDS             10174..10671
                     /locus_tag="LNE35_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6911421.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="LNE35_002304"
     tRNA            complement(11056..11129)
                     /locus_tag="LNE35_002304"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="LNE35_002305"
     CDS             11316..11639
                     /locus_tag="LNE35_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911422.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="LNE35_002306"
     CDS             11803..12057
                     /locus_tag="LNE35_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911423.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="LNE35_002307"
     CDS             12127..12372
                     /locus_tag="LNE35_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6911424.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="LNE35_002308"
     CDS             12448..12813
                     /locus_tag="LNE35_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911425.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="LNE35_002309"
     CDS             complement(12874..13437)
                     /locus_tag="LNE35_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911426.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4087 a         2577 c         2304 g         4975 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atg
//
LOCUS       DAFCNJ010000062        13917 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000062 DAFCNJ010000000
VERSION     DAFCNJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.064"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="LNE35_002310"
     CDS             131..505
                     /locus_tag="LNE35_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6911427.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LNE35_002311"
     CDS             complement(619..1248)
                     /locus_tag="LNE35_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6911428.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LNE35_002312"
     tRNA            complement(1382..1454)
                     /locus_tag="LNE35_002312"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LNE35_002313"
     tRNA            1668..1741
                     /locus_tag="LNE35_002313"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="LNE35_002314"
     CDS             complement(1879..2043)
                     /locus_tag="LNE35_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911429.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="LNE35_002315"
     CDS             2338..3666
                     /locus_tag="LNE35_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBK6911430.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="LNE35_002316"
     CDS             3868..4701
                     /locus_tag="LNE35_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBK6911431.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="LNE35_002317"
     CDS             4717..5454
                     /locus_tag="LNE35_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBK6911432.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="LNE35_002318"
     CDS             complement(5609..6577)
                     /locus_tag="LNE35_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBK6911433.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="LNE35_002319"
     CDS             6809..7642
                     /locus_tag="LNE35_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBK6911434.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="LNE35_002320"
     CDS             7676..8515
                     /locus_tag="LNE35_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6911435.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="LNE35_002321"
     CDS             8541..9101
                     /locus_tag="LNE35_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK6911436.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="LNE35_002322"
     CDS             9300..11021
                     /locus_tag="LNE35_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBK6911437.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="LNE35_002323"
     CDS             11186..12328
                     /locus_tag="LNE35_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK6911438.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="LNE35_002324"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="LNE35_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBK6911439.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAFCNJ010000063        13810 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000063 DAFCNJ010000000
VERSION     DAFCNJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.065"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(221..1423)
                     /locus_tag="LNE35_002325"
     CDS             complement(221..1423)
                     /locus_tag="LNE35_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6911440.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="LNE35_002326"
     CDS             complement(1426..1620)
                     /locus_tag="LNE35_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBK6911441.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="LNE35_002327"
     CDS             complement(1667..2515)
                     /locus_tag="LNE35_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HBK6911442.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="LNE35_002328"
     CDS             complement(2720..3070)
                     /locus_tag="LNE35_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911443.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="LNE35_002329"
     CDS             3665..4111
                     /locus_tag="LNE35_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBK6911444.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="LNE35_002330"
     CDS             4735..5727
                     /locus_tag="LNE35_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6911445.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="LNE35_002331"
     CDS             5743..6627
                     /locus_tag="LNE35_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6911446.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="LNE35_002332"
     CDS             6676..8283
                     /locus_tag="LNE35_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6911447.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="LNE35_002333"
     CDS             8284..9768
                     /locus_tag="LNE35_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBK6911448.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="LNE35_002334"
     CDS             9945..10937
                     /locus_tag="LNE35_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6911449.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="LNE35_002335"
     CDS             10989..11858
                     /locus_tag="LNE35_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6911450.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="LNE35_002336"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="LNE35_002336"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBK6911451.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAFCNJ010000064        13730 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000064 DAFCNJ010000000
VERSION     DAFCNJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.066"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            39..1451
                     /locus_tag="LNE35_002337"
     CDS             39..1451
                     /locus_tag="LNE35_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBK6911452.1"
                     /translation="MSQLLIATPFHVLASENSTYKIAKERTETTEMSNSDVTAESSEL
                     LTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESST
                     ISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQS
                     QPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQL
                     AQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLT
                     KGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEV
                     KGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFH
                     YSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEIC
                     KDSSLSQSMLVDPKTLLFGD"
     gene            1800..2432
                     /locus_tag="LNE35_002338"
     CDS             1800..2432
                     /locus_tag="LNE35_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBK6911453.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3020..4459
                     /locus_tag="LNE35_002339"
     CDS             3020..4459
                     /locus_tag="LNE35_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6911454.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4754..4972
                     /locus_tag="LNE35_002340"
                     /pseudo
     CDS             4754..4972
                     /locus_tag="LNE35_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5221..5436
                     /locus_tag="LNE35_002341"
     CDS             5221..5436
                     /locus_tag="LNE35_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911455.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5653..5909)
                     /locus_tag="LNE35_002342"
                     /pseudo
     CDS             complement(5653..5909)
                     /locus_tag="LNE35_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6096..7007)
                     /locus_tag="LNE35_002343"
     CDS             complement(6096..7007)
                     /locus_tag="LNE35_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6911456.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7026..8030)
                     /locus_tag="LNE35_002344"
     CDS             complement(7026..8030)
                     /locus_tag="LNE35_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK6911457.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8027..10150)
                     /locus_tag="LNE35_002345"
     CDS             complement(8027..10150)
                     /locus_tag="LNE35_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6911458.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10155..12602)
                     /locus_tag="LNE35_002346"
     CDS             complement(10155..12602)
                     /locus_tag="LNE35_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6911459.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12589..12978)
                     /locus_tag="LNE35_002347"
     CDS             complement(12589..12978)
                     /locus_tag="LNE35_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6911460.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13038..13541)
                     /locus_tag="LNE35_002348"
     CDS             complement(13038..13541)
                     /locus_tag="LNE35_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK6911461.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13554..13715)
                     /locus_tag="LNE35_002349"
     CDS             complement(13554..13715)
                     /locus_tag="LNE35_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911462.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4220 a         2710 c         2509 g         4291 t
ORIGIN      
        1 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
       61 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      121 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      181 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      241 gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt agtacgactt
      301 caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca agtaccgaat
      361 cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca aaaccaaacc
      421 atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa actttttcga
      481 caccacagca aatagaatca acagaatctt tccaatcaca accaatacta tctggtaatg
      541 aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa attggtgagt
      601 ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt gcacaagcga
      661 tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac aatttatttg
      721 ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa gatttgggta
      781 atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat gaggaaagct
      841 ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat ttttatgcag
      901 gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt acaggacggt
      961 atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc tacgatttaa
     1021 ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac caagtgccta
     1081 ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt caccgaggac
     1141 ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt aaggttgtta
     1201 aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa gatggatcaa
     1261 ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat gttttgcaag
     1321 gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac cttcatttgg
     1381 aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa accttattat
     1441 tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt ggattcttag
     1501 acctttgatc aggatattga gactggaatt gatatctcga ttacgatggg tttgacaaat
     1561 aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct catattttga
     1621 gcaggtttca ctagatgaaa acaatttatc tatcttgagg atttcgtgta cgtatcgttc
     1681 agtcttgtat tgtaaattag tcataaaatc agactaagaa aaattggaaa tatttttttc
     1741 tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg gtaggtgcag
     1801 tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat ggatataaaa
     1861 aatatcgttg cctatgtgac tgtgggaatg aaacgattgt atatcgatca aacttactat
     1921 caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa tataaaaatt
     1981 tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg cgaagtgaag
     2041 gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta tcggcaaagc
     2101 gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag catcacattg
     2161 aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac tgttcatgga
     2221 aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg tgagataagt tatagtaact
     2281 tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca aggacatgtg
     2341 ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat acaagtggtg
     2401 ttaagggagt ttatcgttct aaagagaaat agattgcgta tataacttta aaaaaaaaga
     2461 ggtactattt aggatcgtat gattctatta agagtgcagc tcaagctaga aaagatgctg
     2521 aaaataaatt gtttcaacct ttgattgaca aatatcgagg taatcattaa ttattgtgga
     2581 taataattaa cagtgatata taaacatcta taatatatta ttcatactat tgatatttag
     2641 tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt ggaaaacaaa
     2701 taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg
     2761 tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt ttagaaattg
     2821 ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa ataaagcatc
     2881 tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt cctatggcta
     2941 gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc tacacgatca
     3001 ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg
     3061 ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg atggcagtat
     3121 tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga atcaagcgga
     3181 atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat
     3241 tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct ttgtcttggg
     3301 atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac gggtggtaca
     3361 agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg aagtctttcc
     3421 catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat taggctcgat
     3481 tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt acattttttg
     3541 gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct taccaaaaga
     3601 tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg cggctacgat
     3661 tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc atccagtccc
     3721 ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa
     3781 agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta ccatgagctt
     3841 gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga ttccttttta
     3901 tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga tggtctttcc
     3961 tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg ttggcccgga
     4021 gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt atatgttttt
     4081 gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg gtgccggcaa
     4141 tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta tcgaaaactt
     4201 gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca gcgggatttc
     4261 gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc aagtaactaa
     4321 ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct ttttaggcag
     4381 ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa aacaaggagg
     4441 tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg
     4501 tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc gaaaaactca
     4561 agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac aagaagactg
     4621 cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt ttagaggcta
     4681 ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac ccagattatg
     4741 taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat ttccgtctag
     4801 tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa ttcgagaatg
     4861 gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta tcaatatttt
     4921 gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt aggaacaacg
     4981 gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac tcttttatca
     5041 agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct ttagcatttt
     5101 tgatttaggt tccttttcta attataataa gatgaccact acagtaacct actgaaatat
     5161 ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat cttcattctt
     5221 atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga ttttacttcc
     5281 aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt
     5341 gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga atttgctatg
     5401 aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga attacttgcc
     5461 acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg aatagagcat
     5521 acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc tatccccgta
     5581 gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat ttttgaccct
     5641 tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt taattcggtt
     5701 ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca ttcatctaag
     5761 gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc tgacattaca
     5821 tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc aagaagaaaa
     5881 actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat atagactgat
     5941 tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc gagttaccga
     6001 atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata agttacacta
     6061 cttataactt ctaataaaat tagattgagt cattattatt taacatcaac gattttccag
     6121 tttccatttt tttgtaatac taatttgtac tgaaagtagc tagtggtttt tgatacatta
     6181 tcaaggtatt ttatctttgt actcactaga aaattttttc cgttttgtag ataaactgga
     6241 ttctctaact ccgaaaactt taagtcaata ttgattggtc gcatcgcacc attttcaaca
     6301 taatactcca attctttttc agaagctgat ggataaagtt taaaaaatgt ttctaaaaaa
     6361 tcggtgattt cttccgttgt tttggcatca atttctgaat cgttttctac ttgttttgct
     6421 tcatattttg ccttgtctgg ctttccaatc attgttggca aactagttac taaatgattc
     6481 ctgttttcat cttcaaagat tgtcactcga tatgcagaag aaattgattg cgtctttttt
     6541 ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa attcttccga gtgtttttca
     6601 acatctagaa tttttacaga ttgaacttcg gaactcgtgg gaatatctgc tcgaaccata
     6661 tcttggatta aagcgagacc gttttctgta aggtactgtt cgagattact cattctcttt
     6721 tcaattgctt ctttgttatt tttccatgaa aaatattctt tcgcaaaatc ctctgtaaaa
     6781 ctttcaacgc cagaagtatt cactaccttc tcttttataa ccactttttc atgagttgta
     6841 tgttgatcaa tggcggtgaa atttttataa acgccaaaag caagactaca agacaataaa
     6901 agccaaaggg caagcaccat ttttcgatgc ttacccacgg atacaactcg ttcttttttc
     6961 ttttttgggc tcttttcctt ttgattccgt tcaattttta tcttcattct ttttcctctt
     7021 ttctcttatt tgtatcggcc agctccgata agatgctgcc gccaatagtt ttccgttaga
     7081 ttcgtccacc ccactggatc tcctgcatga aacatgcgat tctcccctgc gtaaatgccc
     7141 acatgggtca cataagtacc ggcatcatag gttgaatgaa agaaaactaa atcacctggt
     7201 ttggcatctt ttagatcaat atgagtcatg gcatcatatt gatcctgagc cacccgagga
     7261 agtttcaggt caactttggc ataacaccat tgggtcaagc cactacaatc aaaccccgtt
     7321 tctggacttg agccaccaaa cacataggcc gttccttcat acttaaatgc ctcatcaaaa
     7381 atttcttgga ctattttgtc atcaaactga gttgtagtta ggtattgctt cactaataaa
     7441 cagtaaaaca tattgccata attataccgc cacccaccat tttccttaat cgcgattggg
     7501 ttggggtaat cgactttttg accacctgat ttttcctttg aaaaagattc agctaaagca
     7561 aaactatgct tcttcccgtt tttcccgaca tgatctagat acccgccacc atagttatag
     7621 gcctgaataa tactatcttg atcacaccct agttctttgg aacgcttaac caaagacgca
     7681 aaatagagac agccttgttt gatggatgcc tcggtactta atgaattagg tggtaatccg
     7741 gctgactcag aactttgcat aacgtcttcc attgtgccac cagattccac ttgaataatc
     7801 gccaaaagaa ttggaatata ctctgttata tccgattctt tggcatactt ttccaccatt
     7861 gcacggtgtt tcaatacttc ttctgagaca ctagttccgc caggagaagt gccagaacta
     7921 ccatttccct cactgtcttc accaaacaac aaacccacac acatcaaaat acccataaaa
     7981 gagatggtca gaaaagttaa tgaaataaca cccactttct tcaagttcat tttttctcac
     8041 ctgaccttga ttttggacga tggttcattc ttttttgtac tttttgattg gtctgctttg
     8101 gcagagtgga cctctgtttc acttgttgga aagaattttt cttacttgct gattggcttt
     8161 tcccttttga aatgatttct tgactagtta gttttcgttt gatttcggga ttcagatttg
     8221 ctctcacaat ccgctttggt gcttcttttt tcagcatcat atctttctga ggttgagctg
     8281 ctttatctat tggaatattc gcccttcttt ttccctgacg atttgttgct tcactcagta
     8341 tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct cgcaaatcca
     8401 ctcgatccgc tttttcttgg aacagtccgc gcttgaattc ttctggtgct tttttcgact
     8461 tttcgattcc tttatggaga ttgtatttca aattcgttgg cgtattcctt acctgatcct
     8521 ttacttgttt agtcttatct ttcaccaaag cttgggtatc caaaactttg ccgatttttc
     8581 tcccagccaa attcattcgg gattgtttct tattatttga aactggattc tccttagtta
     8641 agtcagatag tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga gaagaaccgg
     8701 atggcttaaa tttggatttt gattgttcct ttgccaactt atttccgact gctgcaccac
     8761 caccaaaagc caaagctcgt cccacatttc gattgagttg ccgcaatttt cgattcatta
     8821 acatttgcgg ttttcgcatg acgcgtcttc ccattgactg gctatcatta ctctggaggt
     8881 tgaacatact cataatgtcg cccaatttga aatagatacc ggcaaaggtc acaatttgca
     8941 gaaaagcaac catgaaaaat ggataggttg ccgaaatatt gaaaaacatc gtggagatgc
     9001 taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat cataatcgta ttgaataaac
     9061 gaataatcgc cttctttcct aaactttcat aagtcggcaa catggacaat aagaaactaa
     9121 ttggcagaaa catcgcaaag ataatgaaga gaatttgcga gaacaacata atccctgtta
     9181 gaagaaaaac aaagaaggaa atgccaatat tgaacaatac caaaaagacc acggtcccta
     9241 aacggttcat ggtcttggta atactgagat tcgcattgtc attgtcttca atctctgcct
     9301 tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg actcacagat agaatcttgt
     9361 tgacccggtc ttcaccaatt tcttctatgt tggaatcatc aaattgcagt agtaaccaag
     9421 gttgttggac ttgaatcgaa aacaaactat cccgaatcaa atccacacta tcttttcctt
     9481 ggctttctga attgggtaca acgatctcgg ttccaagggt aagggccgct tcactaacat
     9541 ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc ataagcaatg aacgaacccg
     9601 atagtagaaa aatcatcaca aaattaatca cggctcgaac tgctttcgtg gtttctcgtt
     9661 ttaataatcc ggtatacgcc acatacaccc ccagagctaa aatcatcaat aatagaaacc
     9721 caaaataaaa tccggaggtc tggagtccat tttcagtaat accagccaat gtttggatat
     9781 tctttccaat actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc tcttgaacca
     9841 cataacccgt ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta gtaatggcat
     9901 aaagaccata catcacgctt ttccctaagc catcacccca attccaaggc aaccaatccc
     9961 aagagctatc cacaaagaaa tctagttgat agttgttcag tgaatatttg gaatatagat
    10021 ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc ggctaccgta ccaactaaac
    10081 tcaatagaac tatcagagtg agtatgctaa taaaaacgat tcccacaatc cgaaaaagtt
    10141 tctttttcat cccctcactc cattctctct tgaacaggtg gacgggtatc aaaggcatgg
    10201 aagagttctt caaagactgg atgtacctga accacgccta cccgaccata caaatcctgc
    10261 atcaagcatt ggccattttc caaatctcgt aggcgttttt gattgctttc atcttctcga
    10321 tccaagccaa agaattccaa ggttttcttg atttcatgaa tatcggtact gcgaaacgca
    10381 aacttcaacc cgatattatt cttcaagcgt tcatccagta aatcatcact attttgcgtg
    10441 acaaagtaaa ccccggcatt catagcccgc ccagcacgaa cgagcttatt cgataatgtt
    10501 ttcccttgcg ctacttgaag aaaactccac gcctcatcta agtctacaat tttgaagatt
    10561 tctcgattac tatggataaa gtccaaggca aaagtggaaa tgacgattag catggaaaca
    10621 ctaagcaatt ccatggtggt atattcttca aaagtggttt ctgcatctgg taaaaccaaa
    10681 tccgctactt gaatgatatt taactgccta tctaaactaa tcgactgggt aatcgaacca
    10741 tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg atccgcgata
    10801 ttttccgcaa ctggtgagcc ttcttttcga agctcattaa ttacttgtaa taatccccgt
    10861 tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt ctccccatct
    10921 cgactagaaa ttcctgttaa aaaggtcaga atatcaattg ctagactttc agaatccttc
    10981 tttttctcca taatcacata aggatcaagt aatccttgat tttttggctt actagttaag
    11041 ttgagaatat tgatttcatc cgcaatttct ggcaaggttt ctttccagcc accccgttcc
    11101 ccttttggat cgacaatcac agcttgtcca ccaaaaagga cggcgtaata aactaagaga
    11161 ttgttactaa aggactttcc gccaccgagt gaaccgagga atgccgctgc tagcgcattg
    11221 gtaactgaat ccttgacccc ttgtgaggcc aatgccggtt tcaggtaaac attgcgcccc
    11281 gtatctacgt tataaccaaa gtaaatgcct tccaactccc ctaacatctg agtggcgcca
    11341 aaaccaagtc cggcgagaaa atcagacgta acatactgga tataatcatt catgtatcgc
    11401 ttactagaag gtaagaattc ttcatgtagc cccatcatat cgccaaaagg ccggactagt
    11461 tttacattgg tatcatcata aaaatctagc acttcatcac accggcgctt tagttcatct
    11521 actgattcgg cacttactcg aaccacatag ctcagtttgt acatggattc tttggactga
    11581 tccaaggtag tttccaattc atctacggaa tccaatgcat ctaagacatt ggaatttgtt
    11641 tcattgtcag attgataggc atggttgtcc aagtctttta attccttttt cttattgcga
    11701 acggtagcaa gtgccttttt gttcgtcaca atctccacat tcatactagt gtcaattggg
    11761 aatgtaaatt gttgttgctg gtaatagaaa agttctgagg agggaaactc catttccccc
    11821 acaattgtat taattgttaa gtacgctaca tatgattcat gattctcatg ttccatgcat
    11881 aagtaccggg gcttttcttc aatcaaacaa cgactgggac gtaataagtc gtaacgtttc
    11941 accaaagttt ccgacttcag ctttttcttt ggtagctgaa attcatattc ttcaaatggt
    12001 gtaccctttt cgccatagat atgttcaatc aaatacatca gatcactcgg tgtgactcgt
    12061 ctgactttaa atcttcgagc caatttactt tccattagtt tttcaagctt ggtatatcgg
    12121 cgaatttctt cattggataa ggaaacaaaa tcgcccatca aatgatgatt cactccatag
    12181 acaaattctt gaaagccgga gaagaaagat ttcttgagat tctttagatt gacctcttca
    12241 tctgtcgcaa tcagtttgaa gccaataaaa aagcgataat caatctgact atcgccaatc
    12301 atggatacta atgcctctgt ctgtaaatca attcgttgtt tcgctacttc tgttaatcgc
    12361 ccggtaattt ccttttttga tcgttcttgc gtggcacgaa tactactttc tgtggcaatt
    12421 tgtaaggcat gaatcttgcc ttcacgattt tgcgcaatca attgacgaaa gttatcgtgg
    12481 acttgatatt tctgttcagg tgacaaaaag gagtaattgt aaggtattaa ctcatagtag
    12541 gcaaaacatt ctccctcttg attaaagaca agattatttt caatatactt aatcgggtac
    12601 atagttcaca ctccttacca gagtaatggg ttcgtcccat cgtttcttct gttctttgac
    12661 tttctttcct gcgaaagtga ccttaaatcg taagtgatag ctaataaaag atcgtaaaaa
    12721 tcccaatggt ttcttaccgt caaacgtttt ttgcgacatg aaccaagtaa ctgccaccgg
    12781 tatacctaag tattttaaaa aggccccctc tatcatagat agtggaggca catgggcaaa
    12841 tacgatgaca aacagcaagg ataaaacaaa ccaagccatc tggttaaaag tcacaggaaa
    12901 aggcagttga aagtcattaa ttgcataaat gaccttttca actgcccaaa tactggtgta
    12961 gctttttatt ttcttcatca aatcattcct ttaaaaagaa ggatagttta gaaatcaact
    13021 acccttcaat aattttgtta atatggatac tcaaaaacac ctcgactcgt taccaaataa
    13081 ttgcctccaa tctccaagtc tctaccacac gcatcaaaat caatatacgt cttcaagtgt
    13141 tctggtactt ctccaaaatc accagcttct acaatcaaga aatatgctac atcactcata
    13201 tcatcgcaat ctggataata gataatatca tccacatgct ctaccatctc ctcaaaagaa
    13261 ttgaagaaag catgttgaat ctcagaagca acttcaccaa ctggcgttcc ttcaagttcc
    13321 tccgctaagc cacacaaccg attaatttcc tcaattggcg tatactcatc aatctcaaag
    13381 ggaagttcat aatcatgaat tgcatattcc tcgtattcgt catttaaccc aattcgttct
    13441 ttcacttcat caaaatcgac aggtggggta aaccacgccc ccaccaattc accttcgtta
    13501 tatttcccga ggttcgcaat ataaacacgc atctgttcca tcgtgcttcc cccttacgca
    13561 ccgacaattc ggttaaacaa actgagtaag acatccttca ccccagaagc attaaagacc
    13621 aatccgaccg caatcaaagc aatcaccaaa aagccaatca gtttggaaaa ctcccgctta
    13681 aatcctaaaa acaaaccaat gaccacaatt gccagtaaga ctaaggattg
//
LOCUS       DAFCNJ010000065        13429 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000065 DAFCNJ010000000
VERSION     DAFCNJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.067"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..914)
                     /locus_tag="LNE35_002350"
     CDS             complement(<1..914)
                     /locus_tag="LNE35_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBK6911463.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="LNE35_002351"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="LNE35_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBK6911464.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="LNE35_002352"
     CDS             2006..2176
                     /locus_tag="LNE35_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911465.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="LNE35_002353"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="LNE35_002353"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBK6911466.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="LNE35_002354"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="LNE35_002354"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBK6911467.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="LNE35_002355"
     CDS             4440..5321
                     /locus_tag="LNE35_002355"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBK6911468.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="LNE35_002356"
     CDS             5318..6034
                     /locus_tag="LNE35_002356"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBK6911469.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="LNE35_002357"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="LNE35_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBK6911470.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="LNE35_002358"
     CDS             6751..7752
                     /locus_tag="LNE35_002358"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBK6911471.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="LNE35_002359"
     CDS             7749..9110
                     /locus_tag="LNE35_002359"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBK6911472.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="LNE35_002360"
     CDS             9070..10221
                     /locus_tag="LNE35_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBK6911473.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="LNE35_002361"
     CDS             10196..11365
                     /locus_tag="LNE35_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6911474.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="LNE35_002362"
     CDS             11540..12919
                     /locus_tag="LNE35_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6911475.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="LNE35_002363"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="LNE35_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAFCNJ010000066        13396 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000066 DAFCNJ010000000
VERSION     DAFCNJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.068"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(15..707)
                     /locus_tag="LNE35_002364"
     CDS             complement(15..707)
                     /locus_tag="LNE35_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK6911476.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1144..1713)
                     /locus_tag="LNE35_002365"
     CDS             complement(1144..1713)
                     /locus_tag="LNE35_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6911477.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="LNE35_002366"
     CDS             complement(1787..2110)
                     /locus_tag="LNE35_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911478.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="LNE35_002367"
     CDS             complement(2264..3076)
                     /locus_tag="LNE35_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911479.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="LNE35_002368"
     CDS             3430..4278
                     /locus_tag="LNE35_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBK6911480.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="LNE35_002369"
     CDS             4423..5364
                     /locus_tag="LNE35_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBK6911481.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="LNE35_002370"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="LNE35_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="LNE35_002371"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="LNE35_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBK6911482.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="LNE35_002372"
     CDS             complement(9828..12014)
                     /locus_tag="LNE35_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBK6911483.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="LNE35_002373"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="LNE35_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBK6911484.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="LNE35_002374"
     CDS             complement(12236..12676)
                     /locus_tag="LNE35_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBK6911485.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="LNE35_002375"
     CDS             complement(12750..13289)
                     /locus_tag="LNE35_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6911486.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3763 a         2807 c         2381 g         4445 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaa
//
LOCUS       DAFCNJ010000067        13212 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000067 DAFCNJ010000000
VERSION     DAFCNJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.070"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            147..1016
                     /locus_tag="LNE35_002376"
     CDS             147..1016
                     /locus_tag="LNE35_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBK6911487.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="LNE35_002377"
     CDS             1225..2250
                     /locus_tag="LNE35_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK6911488.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="LNE35_002378"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="LNE35_002378"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBK6911489.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="LNE35_002379"
     CDS             3333..5036
                     /locus_tag="LNE35_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6911490.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="LNE35_002380"
     CDS             5038..7071
                     /locus_tag="LNE35_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6911491.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="LNE35_002381"
     CDS             7082..7522
                     /locus_tag="LNE35_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6911492.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="LNE35_002382"
     CDS             complement(7722..8849)
                     /locus_tag="LNE35_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6911493.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="LNE35_002383"
     CDS             complement(8852..9529)
                     /locus_tag="LNE35_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6911494.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="LNE35_002384"
     CDS             9793..11559
                     /locus_tag="LNE35_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBK6911495.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="LNE35_002385"
     CDS             11574..12011
                     /locus_tag="LNE35_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6911496.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="LNE35_002386"
     CDS             12024..13175
                     /locus_tag="LNE35_002386"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBK6911497.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAFCNJ010000068        13133 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000068 DAFCNJ010000000
VERSION     DAFCNJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.071"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..694
                     /locus_tag="LNE35_002387"
     CDS             <1..694
                     /locus_tag="LNE35_002387"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HBK6911498.1"
                     /translation="LVTVAKRPLDRIDQEAVFCQELAIQGTLLPLTETYLKEYFPELH
                     ATEAFTVLQAFFLSRVINHLIAPIQNKIMAETIEKMKQDSKEIYAKNNSFLVASLSEL
                     KITDTYLPDIATSLTLLVRRLKERYLQDRKKIIFLLEGDLYVCQSIRSRVLHYFGKRH
                     DLFFMTIQALSDEFLAQEDIDLVVTNYSDYLSEYTVNTDYLLLKTVPDSQDWRLLFQK
                     IEPQLSTLIGLN"
     gene            complement(752..1747)
                     /locus_tag="LNE35_002388"
                     /pseudo
     CDS             complement(752..1747)
                     /locus_tag="LNE35_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558311.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1848..3062
                     /locus_tag="LNE35_002389"
                     /pseudo
     CDS             1848..3062
                     /locus_tag="LNE35_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738897.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(3156..3460)
                     /locus_tag="LNE35_002390"
                     /pseudo
     CDS             complement(3156..3460)
                     /locus_tag="LNE35_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738896.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3974..5487)
                     /locus_tag="LNE35_002391"
     CDS             complement(join(3974..5326,5329..5487))
                     /locus_tag="LNE35_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6911499.1"
                     /translation="MYTYEERKKAVDLYFKYDQALAATTKKLGYPSVGALRQWIREFQ
                     SSDKLHKNIRTSWKYTLAQKEVAVNHYLDYGQCYARTIRKFGYPNKTTLRKWLEEYAP
                     STRKVRQNSIHYTKQEKEAAVLALCTRSLSAKSIANDFGVTRQALYLWKNKLLGKGAT
                     KPLKSMNVTPEIKLLKEERDILQREVYKLRLEKEILEQASDLIKKEPGIDPIILTNKE
                     KTQVIDALCSKYSVIELLRILDLPKSSYYYHRKQLAIPDKYLKERSLIVQLFHDNKGR
                     YGYRRIYCSLKLLGITLSEKVVRRIMTEEKLKVKSLRLRKYNSYGGEITPAVPNLLKR
                     DFKADQPNKKWLTDITEFHIPSGKVYLSPMIDCFDGAIVSWTISTKPNADMVNSMLEN
                     AIATLNAKDSPIVHSDRGAHYRWPGWIKRMKEACLVRSMSKKGYSPDNSACEGFFGRL
                     KNEFFYGQDWKSTSIDQFITDLDNYILWYNKKRIKCSLGGLSPLDYRKKLGIL"
     gene            complement(5810..6652)
                     /locus_tag="LNE35_002392"
     CDS             complement(5810..6652)
                     /locus_tag="LNE35_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6911500.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGYGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTEIAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            6977..7393
                     /locus_tag="LNE35_002393"
     CDS             6977..7393
                     /locus_tag="LNE35_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6911501.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIRESQQSVIDCRKLLVEQLTGGDDL"
     gene            7390..7860
                     /locus_tag="LNE35_002394"
     CDS             7390..7860
                     /locus_tag="LNE35_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6911502.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            7874..8647
                     /locus_tag="LNE35_002395"
     CDS             7874..8647
                     /locus_tag="LNE35_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6911503.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            8661..9473
                     /locus_tag="LNE35_002396"
     CDS             8661..9473
                     /locus_tag="LNE35_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6911504.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            9527..10492
                     /locus_tag="LNE35_002397"
     CDS             9527..10492
                     /locus_tag="LNE35_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6911505.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            10629..>13133
                     /locus_tag="LNE35_002398"
     CDS             10629..>13133
                     /locus_tag="LNE35_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6911506.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSN"
BASE COUNT         4315 a         2101 c         2456 g         4261 t
ORIGIN      
        1 tttagtaacg gttgctaagc ggccgttgga tcggattgat caggaggcag tcttctgtca
       61 ggagttggcg atccaaggga ctttacttcc attaacagaa acgtatttaa aggaatattt
      121 tccagagttg cacgcaacag aagcatttac tgtcttgcaa gcattctttc tttcccgagt
      181 aatcaatcac ctaatcgccc ctattcaaaa taagattatg gcagaaacga ttgagaaaat
      241 gaaacaagat agtaaagaaa tttatgcgaa aaataattcg tttctggttg cttctttatc
      301 ggaattaaaa ataaccgaca cttacttgcc tgatattgct acgagtttaa cgttattagt
      361 cagaagattg aaagaacgtt atctacaaga tagaaagaag attatctttc tgcttgaagg
      421 ggatttgtat gtctgtcaaa gtattcgttc aagagttctt cactacttcg gcaaacgtca
      481 tgacctattc tttatgacga ttcaagctct aagtgatgag ttcttagcgc aagaagatat
      541 tgatttagtc gtaacgaatt acagcgatta tttgtctgag tacacagtaa atacagatta
      601 tctactatta aaaaccgtac cagatagcca agattggcga ttgctattcc aaaaaattga
      661 accacaactg agtacgttga ttggtctgaa ttaacttgaa gcctatttaa cttttttcgt
      721 taagtaggct ctttttttgc gtcttgaatt gtcaaatcaa gtgcaacact ttattaaaga
      781 agacttcata aggtgttttc caacctagac actttctagg tctgctattt agtttttcaa
      841 ccatccgata aatcgttgcg tcatctaccg tgtccatctc ttttgatttc ggtaagtact
      901 ctcgaatgag tccattcgta ttttcatttg ttcctcgttg ccaaggggca tgcggatctg
      961 gaaaataaaa gggcacatta ttcaatgctt ctgtaaccgc gaaatgattg gagaattctt
     1021 ttcctctatc tggagtgatc gttttgacca catcattggg gatactgctt aaaagttcaa
     1081 tcattttttc gctcacgtaa gcagctttct ttcttggtac ctttccagct aaaaggaatc
     1141 gcgattttcg atctgtcaac gtcactaaac aagcaccacc agtttttcca gctacagtat
     1201 cggcttccca atgtccaaat tcacttctat cgtctgcaga ttttggacga tcatgaatcg
     1261 aatgcgtaat gcgtattttt ccacgagcct cgacatgatt ttttgtatgt cttgtttttc
     1321 ctcgatggcg taatgaacgg atcagccctc tattgccact cgagagcggt tctgtatcga
     1381 aaataccgtt gtaaatggca cggtagaccg tgataaaact gattgagaag tccacctgtt
     1441 caagcttcaa acgattagag atttcctctg gcgaccattg aaattctaca aataaatgac
     1501 gaacgattga ccaaattcgc ggatcattta gaatagaatg acgccctcag tcctttctac
     1561 gagaagcata ctttttgtct gctttggaag gtgagtaggt tttctccttg tttcttctca
     1621 attcacgtga aatggtagac ggactacgat gaatgtcatt gccgattttt ctaatagaaa
     1681 caccttcttc atgaagtaga aagattcttt ctcgttcgag tatggtaaga tgatggtact
     1741 ggctcataat tgtcttcctc ctagtagtgt tctcgacaaa cctattctag ttggatattg
     1801 attatgagtc attttttagg tgttgcactt aaagtgtaaa ttcaaaacac aaaaaaaagg
     1861 tgttagtaaa atataaggtt aacgacgcag ggagcaggcc tgtcaaaatc aagccgcggg
     1921 aactttacag agcttcacat ttagattctt gcatctacag atactacgca gaatgtctaa
     1981 acaaagatta tgaaaaagta gcagaaataa gaagaattgg tgataatgtt gttgcttata
     2041 gggttggaaa aggtaaatca aacatagact atgcggctga agcaatagat tttatcaaaa
     2101 gaagcaatga tggctgttat atttttttag gagattttga aaaatttttt gatactttgg
     2161 atcatgaata tttaaagtgc aaattaaaag aagtcttaag tatgagcaca ttggatgaag
     2221 attggtttaa aatttttaaa acaataacta gtttttcttg ggttgaacaa aaagatttaa
     2281 atgatttatt cggttcagaa aaagaacaat ataagaatgg tttaaaatct tattttgata
     2341 cgtgggaaga atttaggaat tttaaagcag atggtcatgt atgtaaaaat actaacagtt
     2401 ttggaattcc tcaaggaacc ccaataagtg ctttgctatc aaatatatat atgatagatt
     2461 ttgacgattg gctaaataaa ttatcatcca aatatggtgg gaaatattta agatatgcgg
     2521 atgatttcat tttgatactg ccctttagta caacctctaa ggacattttt cttgaaatta
     2581 tccgagagat tctgcaatac gcatccgagg tcactaagtt aaaaattgag agaaataaaa
     2641 cagagaagta tctttttaca aatgattcag tatatttgtt tgagtcaaat aaaaatagta
     2701 catcaagtct agtaaaatca aaattggatt acttgggatt tgtatttgat ggaagccaag
     2761 taaagatgag acaaaaaagt gtttcaaaat tttatagaaa agctgataaa gcgatcagag
     2821 ctgctagaga aaaaagtcga attaagaaat cgagttatct aattggtaaa aggcatattt
     2881 atagatattt ttttgatgtg ggtgtaaggg atagtaaagg gagtaaattt ggaaacttca
     2941 tttcctatgc tagaagatgt caaatgaaat ttgatcaaat atctcctctg acggagaatt
     3001 taatgatgga acaaataaaa aacagacgta aaaagctatt aaagaaaatg tataagaact
     3061 gataatcaaa tgatatgctc ccatcatagt agacgttaga aaaaaatatt gataatctac
     3121 tatgatgaga atttatcaaa tcgtgacttt catatctatc gtccattaac cgctgaaaca
     3181 aacaccaaat aattcgcgaa atcgccaatc cgttgtcggt ggtcattcac atcacttctt
     3241 gtacgttgtt cttgttcttc ttgtaagaat aagcgtgatg tccaagaatg actgtataac
     3301 gaatagcagc acccatcaac tcttcccaaa tttcgataaa atccttatca ttcatatcag
     3361 cattttttaa tatttcttga tagatagtgg tcatttcagt aaaagatagc gcttctggtt
     3421 gttgtaaata attttcgtat gtttgattca attgggacat taaatcactc cttgaaatgt
     3481 ttttgtttta actagtttac tacaagaaat aaaagttagt gcatcaaaag aaaaacagga
     3541 aagcatacca ttttggtagt tttttctcgt tgagttggta taaatttgat gagacaaata
     3601 ggctagtatc actttattca ttgatactag cctattttgt atccaaaatt tatgaaaatg
     3661 attaaaacaa atctttttct tgctcaatga attgagcaaa tttatcagca cttcgttctt
     3721 atcgttctgg ataaaaatag ttgtagactt gttttgtcat ttttgaatct ttatgtccaa
     3781 cacgatacat aatatcctat agtttcatgt ctgccattgc gcacaaggaa acgtgagttt
     3841 ttctaaattc atgaagagtt atccgttttt caaggtgata tttattagcg atcatgtcat
     3901 accaatcatt tggtgtcaag ggcatcttaa aattgtaggt ttgggggtgc ggacgttttc
     3961 ctgcaccaaa attttataaa atacctagtt tcttacgata atctaatgga cttaatccgc
     4021 ccaatgaaca cttgattcgc tttttgttgt accaaagaat gtagttatcc aaatcagtga
     4081 taaattgatc aatagatgtc gacttccaat cctgcccata aaagaattca ttcttgaggc
     4141 gcccaaaaaa gccttcacaa gcagaattat caggagaata tccttttttt gacattgaac
     4201 ggactaaaca ggcttctttc attcgtttga tccaacctgg ccaacggtaa tgtgcgccac
     4261 gatcagaatg aacaattgga gaatcctttg catttaatgt ggctatagcg ttctctaaca
     4321 ttgaattgac catatctgca ttgggttttg tgctaattgt ccaactaacg atagctccat
     4381 caaaacaatc aatcattgga gaaagataga ccttcccaga gggaatatga aattctgtaa
     4441 tatcagtgag ccatttcttg tttggttggt cagctttaaa atcacgtttt aaaaggttcg
     4501 gaacagcagg agtaatttct cctccataag aattgtactt tcttaatcga agtgatttca
     4561 cctttaattt ctcttcagtc ataatgcgac gtaccacttt ttcagataaa gtaattccta
     4621 ataacttaag cgaacaataa atacgacgat agccatatct tcctttattg tcatgaaata
     4681 attgcacaat taaggagcgt tcttttaaat atttgtctgg gatagctaat tgtttcctgt
     4741 gatagtaata gctacttttg ggcaaatcaa gaattcttaa tagctcaatt acgctatatt
     4801 tggagcataa ggcatcaatc acttgtgttt tctctttgtt tgtcaatatg attggatcga
     4861 tgccaggctc tttttttatt aaatcagacg cctgttctaa aatttctttt tctaagcgca
     4921 gtttgtatac ttcacgttga aggatatctc gttcctcttt caacaatttg atttcaggag
     4981 tgacattcat tgatttcaaa ggtttagtag ctccttttcc aagcagcttg tttttccata
     5041 agtacaatgc ttgtctggtt acgccaaaat catttgcgat tgatttagca gagagtgaac
     5101 gagtgcacag ggctaggaca gcagcttctt tttcttgttt tgtatagtgt atcgaatttt
     5161 gacgaacttt acgagtagat ggagcgtatt cttccaacca ttttcttaaa gtagttttat
     5221 ttggatagcc aaactttcga attgtacgtg cataacattg cccataatct aaataatgat
     5281 tcacagcaac ttctttttga gcaagcgtat atttccaaga agtacgttga tatttttgtg
     5341 cagcttatct gaagattgaa attctctaat ccattgtctg agggcaccaa cagaaggata
     5401 tccaagtttt ttagttgtag cagccaatgc ttgatcatat ttaaaatata aatcaacagc
     5461 ttttttccgt tcttcatatg tgtacatgag acgtcctcca aacttatttc agtccaagtt
     5521 tttgtccgca tgcccttgta tgtactttta ttctgccata aacagcttaa tgaatgtcca
     5581 cttgaaaaag tgaataaacg aaatagatga ctcgatttaa taaggaagta aacccacaca
     5641 gaaatatagg tgattttcct tgaatgacca agatattgaa agttagtgaa aaaacacaaa
     5701 aaggtagccc tattcacaga aggtctgcct tttgtgttat cgccagcaaa cattagaggg
     5761 gatatttcat atagcttttg tcctctcgac agtcctatca gtttctcttt taacttatct
     5821 ctaagtgctg tccagtttta atagcttcta gcttgtttcc taactgtgtt tttaatatgt
     5881 tgaaggcttt tgaacctgtg cagtggcaag taaggatttt ttctactcgt gagtcattca
     5941 atttatttcc aattttcatt acctcttgct caggtagacc taaattattt atataaggta
     6001 tgccaatcat atggaagcca ccgattaggt attttatggg tttagtcgga aatgtttcaa
     6061 gagcaatttc catcatatcg ataatgccat gatgactaca gccagtaaag acaatcagac
     6121 cgtcttcttg ctcaattacg accaataatt catctgaaaa atcatcataa acaaatttat
     6181 catccatctt tttgtataaa ttcttggtgg tcgagtttgc tttgcctagg gggatgatat
     6241 gaaagctcgg agcaatttct gttttcgttt ttattgtatg taaattctca tgcattttaa
     6301 ggatttcatt tttaggaata ccaacataca gaaacgtggt taaaatcttt aaataatggg
     6361 gtaaagttac accttcagct gtataaatag gggcgtttcc tgattttaaa tatcttaacc
     6421 cgccggtgtg gtcagaatga ccgtgtgaaa taatcactgc ctccagatca ttcaaagttt
     6481 cccccatttt agtcgcatta tagactaatt ttttattagc gccagcatcg aagagatatt
     6541 tcttccctga gtagttaatt aagagagata acccatatcc acttttaaag ttcaaattta
     6601 ttcttgagtt ttcaaataaa accttgattt ccactataac atctcctttc acatgaacgt
     6661 gtattcatat attgtagcac attaagttct attttttgtt gtaattaccg aaaaaaatat
     6721 taatggacgc tttttacgct ttatttcgta tgtggaagaa aatttcttag gatgtggcgg
     6781 gggattgggg caaagctaaa ttccaattac agtgatcatt taattagaca cctttaaata
     6841 cccacacttt catcaacact ataatttagg gaaagaaaag tgttggcatg atatagaaaa
     6901 acctttaatt aacagcgttt cctaaaattg gcacgcatct tgcttataga aaagcgtaag
     6961 gataggagga atgaaaatgg caactataat tcttgccagc catggggaat ttgctcaagg
     7021 attaaagcaa acagcgacaa tgattattgg agatagtgta ccaattcatg ccttgtcagc
     7081 atttagagat gaagatgaat ctattttggt acaaataaaa aaacttttag ccacaataga
     7141 tatagaagaa acctacattt taactgatat tctaggtggt agtgtgaata atgatatgct
     7201 cactattatt aaagagtatg agggattgca attaattact ggaatgaact tacctttagt
     7261 tatttctatt gcaacctata atggaaaaat tggggaaagt gagctagaaa agcttattag
     7321 agagagtcaa caatcagtga ttgattgtag aaaattatta gtagaacaac ttactggagg
     7381 agatgaccta tgattaaatt agtacgtatt gaccatcgtt tgttgcacgg acaggtagta
     7441 ttttcttgga gtaaatcatt aaatatcaat cgtattatta ttgccaataa agaggcagcg
     7501 aatgatgaat tcaaaaaaat gtctttgaat ttatctaaac cgactggcat taaattaaca
     7561 attttttcag ttgaacagtt actagaaaaa atgcctaaaa tcgaaacact taacgaaaat
     7621 atcatgctta tttttggtaa tacaaaagaa attcgtcaat tttgtgaagc ctatcctaaa
     7681 gtacaagaaa tcaactatgg agggataatc aaaaaagaag gttccaagca atttagtaat
     7741 gccattttct tgaatgaatc tgagcaagaa gatgccaaaa aattaaaagc tcttggcatt
     7801 aaacaattta ttcaacaggt accaacttct aaaaaagaag atttgaatac aatgttataa
     7861 aggaggaatg aaaatgctcg ttcagtcaat tggtttaggg ttaatcggtg ttttttgtat
     7921 tttagactca aggcttttag gaagaatgaa ttttgaacga ccgttgattg tcagtacgct
     7981 tgttggtttg gtacttggag acatggaaaa aggattaatt gttggagcct ctttggaatt
     8041 gatgtcctta ggattagtaa atatcggagc tgctgcacca cctgatatga atatggcatc
     8101 aattattgct acagcctttg ccattttatc aaatgcgaat gcagaagcag cattaacgat
     8161 tgccattcca atttcagtgt taggtcaaat actaggtatc ttaatgcgga cgattttgtc
     8221 aaatttgact cacgttgcgg atcgctttat cgaacaagga aaatttagcc aagcaaggag
     8281 aattcatata ctttatggct cgggattata tgctctcatg tattttattc ctatttttct
     8341 agcaatttat tttggaacga atcttgtttc tcagattgtt gatgcgattc ctgagtggct
     8401 aacaaatggc ttaacggttg caagtaaaat tttaccagca tatgggtttg cgttgttatt
     8461 acaaacgatg ttaacgaaaa aaatggtgcc atttttatta cttggatttt taattactgc
     8521 ttattctgga ttgggaatca ttggtattgc agccttcgca tgtattgttg cgtttgtcat
     8581 ggttcaagtt caaggtaaaa actcaaatgg taaccaaaat gagccagatc cattagatga
     8641 cttataagga ggtagattaa gtgaaagaga aagatcaaaa tgcatggaaa tatcggcagt
     8701 tcttatggcg ttcatgggca attcaagcct catggaacta tgaacgacaa atgaacatgg
     8761 gttttatgta cggaattgca ccaattctag ataacattta caagaatcca gaagatgaag
     8821 caatgaagaa agaagcttac gagcggcata tgatgtacta caactgtacc cctcaaacaa
     8881 gtgcatttgt gttagggtta actgcttcta tggaggagca atactacgaa gaccaagaaa
     8941 actttaatcc ggaatcaatc agtgcagtga agacttctct gatgggaccg ctatctggtg
     9001 ttggtgattc tttctttcaa ggaacgattc gggtattagc ctttggacta ggaattaatt
     9061 tagcccaaca aggtagtatt ttaggtccaa ttctagcaat gttaatttcc tttgtacctt
     9121 cctttttagt tacttactat ggtgggaaaa ttggctataa catggggaat aaatacttgt
     9181 ctaaactgta tcaagaaggt ttgatggata aagtaatgta tgtttgctcc attgtggggt
     9241 tgatggtcat tggttcgatg attgccagca tgatcgggat tacaacacca attcaaatgg
     9301 gtaaagcttt tgtcttacaa gatgtattag atagtattat gccacaattt ttaccattag
     9361 gattaacctt cttcatgtac tggttattgc agaaaaaggt caaaacagga tggatgttgg
     9421 caatctgttt agtcggagga attattttta gtttgttagg tatcttacaa taagggaata
     9481 accaataaat ttggttgaat aatataaata aggaggaatt caattgatga attcaaaaga
     9541 aattgttaaa gaaatcgtag cgaaaaaaga ggtaaaatca atcgtatttg ttggctgtgg
     9601 cgcttcaaaa gcggaccttt atcctgcaaa gtattttcta gaccaaaatg cgaagcagtt
     9661 acggattagt cattacacag ccagcgaatt taactatgca acaccagcat ctgtagatga
     9721 gtcaactgtc gttatttcag cttctttagg aggtacgact ccagaaacag ttgaagctaa
     9781 cgcagtagct aaagaaaaag gtgctacagt tattagtcta acacgaagca ttgactcacc
     9841 gttaacaaaa gatgcagatt atgtaattta tcatcgattt gcagagaact atgctgctaa
     9901 attagaaaaa acaggctact gtttagaatt ggcagtagaa cttttacatc aagtagaagg
     9961 ttatgaccat tatgaagcaa tgcaagaagg atttgctact atctatgatc ttgcccaaaa
    10021 aagtgcagaa tcagctcgta aagatgcgaa gaaatttgct gaacgcttta aagatgaaaa
    10081 agttatttat gtaatgagta gtggtgctac acatgaagta gcatactcaa catcaatttg
    10141 cttgatgatg gaaatgcaat ggattaattc tggtaccttc cattcaggag aatttttcca
    10201 cggaccattc gaaattgtgg ataaagacgt accgtttata ttattaatga atgatggaaa
    10261 aactagaaat atagatgctc gagcattaac tttccttgaa cgctttgata cgaaaacaga
    10321 agttgtagat gccttggatt atggcttgtc ttcacatatt gctaaagaag tcttagacta
    10381 ctttaaccca ttcgtgatta ctgcagtatt tagagtttat gcagaagaat tgtcagaagc
    10441 aagagaacat ccattaacta agagacgtta tatgtggaaa ctaacttatt aaaaaataga
    10501 tcaaactaaa taaggtggct tgagaaatta agccaccttg ttttcttcaa tgtgatttta
    10561 tacatatggt aaaataacta gtacatatgg taaaataact agtacatatt gtgaaagcag
    10621 gtggcaagat gcagcgatta aatgactatc tttatcaact aacattgctt ttgaaatcta
    10681 cgccttcaga taattattca gctcgcagta tttcgcaagc tttacagttg aatagaagta
    10741 caatcagtag ctacttaaat gaaggtgttc gtgaagggct tttcataaaa gtaaaaagtt
    10801 acccggtttt atttttacat agaacagctc ttgaagaact acatatcaca ctgaataact
    10861 ccgaaataga atcaattgaa agcttgttaa caatttctca aaaacccgca ttagatcaag
    10921 taattggctc taaaggaagt ttgaaagaag caattgatca aattaaaacg gcagtcttat
    10981 atcctggaaa agggttgcct ttattattga tcggcgctag tggatcaggg aaaacctttc
    11041 tagcgaataa aatatatgaa tatgcagtag aagaaaaggt cattcacaca tcggcacctt
    11101 tcatagatta taattgtgca caatatgtga gcaatccaga gttactatcc tctgctttat
    11161 ttggctacac gaaaggagca tttaccggag caagccagga gcatactggt ttgctagaaa
    11221 aagccgatgg aggagtattg tttttagatg aagtgcatcg cttatctgaa gaagggcaag
    11281 agaaattatt tacatttatg gatacgggcg aattctctcc tatgggggat aatagtattc
    11341 ggaaaaaagc cgatgttcga ttagtctttg caacaacaga aaatatatat acgactttct
    11401 taccaacttt tcttaggaga ttgccagtta ttgttaatct acctagattt caacaacgtc
    11461 cgtcgtttga acgtctgtca ttgattgatg aattttttgt ttctgagagt aaaattttag
    11521 ctaaggaact atctgtttct gatgcattga ttcatttctt aatgaatagt gattttgaag
    11581 gtaacgtggg gaatattaaa aatattatca agtatgcttg cggaaacgca tatatccacc
    11641 agaagagtca gcaaacaatt caagttaaat tattggatat gcctttagag tacagttata
    11701 aattcaagga gcaactaaat aaacccagaa agaaaagagc agatcgaatc tactatccag
    11761 aagaaacacg acaagtgcaa ctagaaagaa acaatcagct ttttgttcaa ttttttgacc
    11821 aattacttac tggattctct gaagtgattg aaaataacaa ggatattaaa ctatttttag
    11881 aagaaatggt taccaaggta acacaagtaa tggatacgct tatttttcaa gaggattacg
    11941 aaaaagagca gtctttatac gccattctaa actatcatat tcgtcaaagt attgatttta
    12001 tgcgagaaag ctacgggttt gaacaagatg gtaaccgggt agtcgctttg gccaactatc
    12061 tatatgtcaa ggataatcaa gtgatttttg aaaatagtct tcaagaaaag aaaaccaaag
    12121 atcagcttaa cgaatttttt gctaatgttc ttgaaacccc ttattggtat gcaaaaaaaa
    12181 tcttaagcct tttatctgag tgtttagatc aacctgtgta tgaagaggac attttcttta
    12241 ttacgctata tttttatagt ttgaatgtca cgaatgttaa aacaggtatt aagagtattg
    12301 tgctagctca tggttattct acagctagta gtatggcgaa tgttgccaac cgtatgctga
    12361 ataagaacgt ttttcaagct tatgatatgc ctttagatat tacattagag aaaattgaag
    12421 caaaattgat tcaattcctt ggagattatc gaacagattc aggcttgatt ttattggtag
    12481 atatgggttc actaaaccaa ctaggcggtt tattagtgaa tcatctaaat ggaccactgt
    12541 taatcattga tcatatcaat acaccgttat tattggaagt cggccaacaa gtattaaaca
    12601 ataaaagtat caccgaaata ggagcagaaa ttgaaacaag tcttactttc aaaaagcagt
    12661 tactacttcc caagaaaaag aaaaagaaag ccatcattac ttgttgttat acagggattg
    12721 gtagtgcgac ccagattcag gagattttaa cgaaatgctt gaaagatgca gttagagaat
    12781 tagtaattat tccgtatgat tacaaaaagc tgttagaaaa caagaatttt gagactcctt
    12841 ttcaattata cgatgtcatc atgattgtag gtacagaaga tcccaaaatt aatcagattc
    12901 catatattgg attagatcgc ctgattaatg gggaagcaat tggcgaattt gttcattact
    12961 tggaaaaaga gctgactatt gaagtgaacg atttacaaaa agaattaatt ttcaattttt
    13021 caattagtaa aattgtcgaa aatttaacca ttttggatcc agataaggtg ctatcttttg
    13081 tacaaaaagc aattaaagaa ttatcgaagt tattaaattt tgatttttct aat
//
LOCUS       DAFCNJ010000069        12735 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000069 DAFCNJ010000000
VERSION     DAFCNJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.072"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="LNE35_002399"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="LNE35_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK6911507.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="LNE35_002400"
     CDS             complement(323..892)
                     /locus_tag="LNE35_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBK6911508.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="LNE35_002401"
     CDS             1078..1980
                     /locus_tag="LNE35_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6911509.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="LNE35_002402"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="LNE35_002402"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBK6911510.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="LNE35_002403"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="LNE35_002403"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBK6911511.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="LNE35_002404"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="LNE35_002404"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBK6911512.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="LNE35_002405"
     CDS             8548..8733
                     /locus_tag="LNE35_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBK6911513.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="LNE35_002406"
     CDS             complement(8795..9991)
                     /locus_tag="LNE35_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK6911514.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="LNE35_002407"
     CDS             complement(10161..11597)
                     /locus_tag="LNE35_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK6911515.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="LNE35_002408"
     CDS             11890..12699
                     /locus_tag="LNE35_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6911516.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4009 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctagtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca cgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgaca
//
LOCUS       DAFCNJ010000070        12586 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000070 DAFCNJ010000000
VERSION     DAFCNJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.073"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="LNE35_002409"
     CDS             6..305
                     /locus_tag="LNE35_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6911517.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LNE35_002410"
     CDS             378..1739
                     /locus_tag="LNE35_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK6911518.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LNE35_002411"
     CDS             1761..2021
                     /locus_tag="LNE35_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911519.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LNE35_002412"
     CDS             2035..2787
                     /locus_tag="LNE35_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBK6911520.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LNE35_002413"
     CDS             complement(2881..3486)
                     /locus_tag="LNE35_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6911521.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LNE35_002414"
     CDS             complement(3539..4513)
                     /locus_tag="LNE35_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBK6911522.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LNE35_002415"
     CDS             complement(4891..5610)
                     /locus_tag="LNE35_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6911523.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LNE35_002416"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LNE35_002416"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK6911524.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LNE35_002417"
     CDS             7161..8558
                     /locus_tag="LNE35_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6911525.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LNE35_002418"
     CDS             8639..9079
                     /locus_tag="LNE35_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6911526.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LNE35_002419"
     CDS             9227..9553
                     /locus_tag="LNE35_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6911527.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LNE35_002420"
     CDS             9554..10303
                     /locus_tag="LNE35_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911528.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LNE35_002421"
     CDS             complement(10383..10838)
                     /locus_tag="LNE35_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK6911529.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LNE35_002422"
     CDS             complement(11265..11540)
                     /locus_tag="LNE35_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911530.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..>12586
                     /gene="thiD"
                     /locus_tag="LNE35_002423"
     CDS             11834..>12586
                     /gene="thiD"
                     /locus_tag="LNE35_002423"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBK6911531.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIRE"
BASE COUNT         4020 a         2164 c         2565 g         3837 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaa
//
LOCUS       DAFCNJ010000071        11667 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000071 DAFCNJ010000000
VERSION     DAFCNJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.074"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..216)
                     /locus_tag="LNE35_002424"
     CDS             complement(37..216)
                     /locus_tag="LNE35_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911532.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="LNE35_002425"
     CDS             complement(597..1304)
                     /locus_tag="LNE35_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911533.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="LNE35_002426"
     CDS             complement(1297..1734)
                     /locus_tag="LNE35_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911534.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="LNE35_002427"
     CDS             complement(1749..2000)
                     /locus_tag="LNE35_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911535.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="LNE35_002428"
     CDS             complement(2000..2206)
                     /locus_tag="LNE35_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBK6911536.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="LNE35_002429"
     CDS             complement(2643..2903)
                     /locus_tag="LNE35_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911537.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="LNE35_002430"
     CDS             2978..3181
                     /locus_tag="LNE35_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBK6911538.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="LNE35_002431"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="LNE35_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK6911539.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="LNE35_002432"
     CDS             complement(3810..4709)
                     /locus_tag="LNE35_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBK6911540.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="LNE35_002433"
     CDS             complement(4722..5315)
                     /locus_tag="LNE35_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBK6911541.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="LNE35_002434"
     CDS             complement(5391..5540)
                     /locus_tag="LNE35_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911542.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="LNE35_002435"
     CDS             complement(5556..7760)
                     /locus_tag="LNE35_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBK6911543.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="LNE35_002436"
     CDS             complement(7771..9231)
                     /locus_tag="LNE35_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBK6911544.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="LNE35_002437"
     CDS             complement(9292..11664)
                     /locus_tag="LNE35_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911545.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DAFCNJ010000072        10924 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000072 DAFCNJ010000000
VERSION     DAFCNJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.075"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..281)
                     /locus_tag="LNE35_002438"
     CDS             complement(<1..281)
                     /locus_tag="LNE35_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6911546.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            423..1541
                     /locus_tag="LNE35_002439"
     CDS             423..1541
                     /locus_tag="LNE35_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6911547.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="LNE35_002440"
     CDS             1669..3045
                     /locus_tag="LNE35_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK6911548.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="LNE35_002441"
     CDS             3446..5026
                     /locus_tag="LNE35_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBK6911549.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="LNE35_002442"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="LNE35_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="LNE35_002443"
     CDS             complement(5652..5972)
                     /locus_tag="LNE35_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK6911550.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="LNE35_002444"
     CDS             complement(6120..8354)
                     /locus_tag="LNE35_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6911551.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="LNE35_002445"
     CDS             8833..9099
                     /locus_tag="LNE35_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBK6911552.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="LNE35_002446"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="LNE35_002446"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK6911553.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3480 a         1911 c         2215 g         3318 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aaat
//
LOCUS       DAFCNJ010000073        10723 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000073 DAFCNJ010000000
VERSION     DAFCNJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.076"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(241..1089)
                     /locus_tag="LNE35_002447"
     CDS             complement(241..1089)
                     /locus_tag="LNE35_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6911554.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1088..2155
                     /gene="gnd"
                     /locus_tag="LNE35_002448"
     CDS             1088..2155
                     /gene="gnd"
                     /locus_tag="LNE35_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBK6911555.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="LNE35_002449"
     CDS             2157..3158
                     /locus_tag="LNE35_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBK6911556.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="LNE35_002450"
     CDS             3155..3439
                     /locus_tag="LNE35_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBK6911557.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="LNE35_002451"
     CDS             3472..4269
                     /locus_tag="LNE35_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6911558.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="LNE35_002452"
     CDS             4283..5116
                     /locus_tag="LNE35_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6911559.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="LNE35_002453"
     CDS             5134..5991
                     /locus_tag="LNE35_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6911560.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="LNE35_002454"
     CDS             6013..6522
                     /locus_tag="LNE35_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6911561.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="LNE35_002455"
     CDS             6559..6960
                     /locus_tag="LNE35_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911562.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="LNE35_002456"
     CDS             7072..7425
                     /locus_tag="LNE35_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911563.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="LNE35_002457"
     CDS             7459..7902
                     /locus_tag="LNE35_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6911564.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="LNE35_002458"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="LNE35_002458"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK6911565.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="LNE35_002459"
     CDS             8997..9710
                     /locus_tag="LNE35_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6911566.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="LNE35_002460"
     CDS             complement(9917..10066)
                     /locus_tag="LNE35_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911567.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="LNE35_002461"
     CDS             10462..10662
                     /locus_tag="LNE35_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6911568.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3588 a         1694 c         2320 g         3121 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa att
//
LOCUS       DAFCNJ010000074        10532 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000074 DAFCNJ010000000
VERSION     DAFCNJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.077"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(25..162)
                     /locus_tag="LNE35_002462"
     CDS             complement(25..162)
                     /locus_tag="LNE35_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBK6911569.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="LNE35_002463"
     CDS             complement(159..1343)
                     /locus_tag="LNE35_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBK6911570.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="LNE35_002464"
     CDS             complement(1599..1853)
                     /locus_tag="LNE35_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911571.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="LNE35_002465"
     CDS             complement(1878..3020)
                     /locus_tag="LNE35_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBK6911572.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="LNE35_002466"
     CDS             complement(3080..4432)
                     /locus_tag="LNE35_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK6911573.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="LNE35_002467"
     CDS             complement(4503..4847)
                     /locus_tag="LNE35_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBK6911574.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="LNE35_002468"
     CDS             complement(4857..5231)
                     /locus_tag="LNE35_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6911575.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="LNE35_002469"
     CDS             complement(5244..5558)
                     /locus_tag="LNE35_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6911576.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="LNE35_002470"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="LNE35_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="LNE35_002471"
     CDS             complement(8976..9638)
                     /locus_tag="LNE35_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911577.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="LNE35_002472"
     CDS             10255..10485
                     /locus_tag="LNE35_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911578.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2895 a         2344 c         1786 g         3507 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ct
//
LOCUS       DAFCNJ010000075        10192 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000075 DAFCNJ010000000
VERSION     DAFCNJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.078"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            120..626
                     /locus_tag="LNE35_002473"
     CDS             120..626
                     /locus_tag="LNE35_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBK6911579.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="LNE35_002474"
     CDS             643..1542
                     /locus_tag="LNE35_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBK6911580.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="LNE35_002475"
     CDS             1629..2513
                     /locus_tag="LNE35_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK6911581.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="LNE35_002476"
     tRNA            complement(2705..2776)
                     /locus_tag="LNE35_002476"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="LNE35_002477"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="LNE35_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBK6911582.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="LNE35_002478"
     CDS             4319..5410
                     /locus_tag="LNE35_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBK6911583.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="LNE35_002479"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="LNE35_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK6911584.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="LNE35_002480"
     CDS             6562..7752
                     /locus_tag="LNE35_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBK6911585.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="LNE35_002481"
     CDS             7856..8611
                     /locus_tag="LNE35_002481"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBK6911586.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="LNE35_002482"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="LNE35_002482"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBK6911587.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2111 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct ca
//
LOCUS       DAFCNJ010000076        10086 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000076 DAFCNJ010000000
VERSION     DAFCNJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.079"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(247..759)
                     /locus_tag="LNE35_002483"
     CDS             complement(247..759)
                     /locus_tag="LNE35_002483"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBK6911588.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="LNE35_002484"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="LNE35_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBK6911589.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="LNE35_002485"
     CDS             complement(3212..3655)
                     /locus_tag="LNE35_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBK6911590.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="LNE35_002486"
     CDS             complement(3656..4444)
                     /locus_tag="LNE35_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6911591.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="LNE35_002487"
     CDS             complement(4441..5382)
                     /locus_tag="LNE35_002487"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBK6911592.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="LNE35_002488"
     CDS             complement(5559..6146)
                     /locus_tag="LNE35_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBK6911593.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="LNE35_002489"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="LNE35_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBK6911594.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="LNE35_002490"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="LNE35_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK6911595.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="LNE35_002491"
     CDS             complement(9063..9998)
                     /locus_tag="LNE35_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6911596.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAFCNJ010000077        10085 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000077 DAFCNJ010000000
VERSION     DAFCNJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.080"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            163..393
                     /locus_tag="LNE35_002492"
     CDS             163..393
                     /locus_tag="LNE35_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBK6911597.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            490..1791
                     /gene="murA"
                     /locus_tag="LNE35_002493"
     CDS             490..1791
                     /gene="murA"
                     /locus_tag="LNE35_002493"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK6911598.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1804..1980
                     /locus_tag="LNE35_002494"
     CDS             1804..1980
                     /locus_tag="LNE35_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK6911599.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2115..2411
                     /gene="yidD"
                     /locus_tag="LNE35_002495"
     CDS             2115..2411
                     /gene="yidD"
                     /locus_tag="LNE35_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBK6911600.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2475..2942
                     /locus_tag="LNE35_002496"
     CDS             2475..2942
                     /locus_tag="LNE35_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6911601.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3027..3911
                     /locus_tag="LNE35_002497"
     CDS             3027..3911
                     /locus_tag="LNE35_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6911602.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4025..4261
                     /locus_tag="LNE35_002498"
     CDS             4025..4261
                     /locus_tag="LNE35_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBK6911603.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4349..4429
                     /locus_tag="LNE35_002499"
     tRNA            4349..4429
                     /locus_tag="LNE35_002499"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4383..4385,aa:Tyr,seq:gta)
     gene            4436..4507
                     /locus_tag="LNE35_002500"
     tRNA            4436..4507
                     /locus_tag="LNE35_002500"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4468..4470,aa:Gln,seq:ttg)
     gene            4632..4802
                     /locus_tag="LNE35_002501"
     CDS             4632..4802
                     /locus_tag="LNE35_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911604.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5022..5729
                     /locus_tag="LNE35_002502"
     CDS             5022..5729
                     /locus_tag="LNE35_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6911605.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5789..6433)
                     /locus_tag="LNE35_002503"
     CDS             complement(5789..6433)
                     /locus_tag="LNE35_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBK6911606.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6560..6847
                     /locus_tag="LNE35_002504"
     CDS             6560..6847
                     /locus_tag="LNE35_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911607.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6955..7275
                     /locus_tag="LNE35_002505"
     CDS             6955..7275
                     /locus_tag="LNE35_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6911608.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7356..8669
                     /locus_tag="LNE35_002506"
     CDS             7356..8669
                     /locus_tag="LNE35_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK6911609.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8666..9385
                     /locus_tag="LNE35_002507"
     CDS             8666..9385
                     /locus_tag="LNE35_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBK6911610.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9513..10064
                     /gene="raiA"
                     /locus_tag="LNE35_002508"
     CDS             9513..10064
                     /gene="raiA"
                     /locus_tag="LNE35_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBK6911611.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1669 c         2151 g         2906 t
ORIGIN      
        1 cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa aaatgtaagg
       61 ggattcctat atttttaagt atacttgttt ttccttttat acattttatg gtattatcat
      121 tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt atacggaatt
      181 gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt ctggtcttta
      241 cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat tagaatggca
      301 atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt agaagttatc
      361 gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac tattttaaaa
      421 gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga attttttgga
      481 gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg aacagtaaga
      541 attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt agcagaagaa
      601 ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat gaatcaagtg
      661 attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt aacaatcgat
      721 gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat gcgtgcttca
      781 atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc tatgcctggt
      841 ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca agctttagga
      901 gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt gatcggtaac
      961 acgatttatt tagatttccc tagtgtaggt gctactcaaa atatcatgat ggctgccgta
     1021 aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat cgttgattta
     1081 gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga aacaatgcgt
     1141 attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga tcgtatcgaa
     1201 gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat tgcagatgct
     1261 atctctgaac ataaccgccc attgatttct aaattgatcg aaatgggtgc agaaatcatt
     1321 gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac agatgtaaaa
     1381 acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc tatccaatta
     1441 gttgcagaag gtacaagtgt agtgactgaa acagtgtttg aaaatcgttt ccaacattta
     1501 gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc aatcatggat
     1561 ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc agcagcagca
     1621 ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt gaattatcta
     1681 gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga cgttgaacgt
     1741 gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta aggaagtggc
     1801 tgaatgagta accaacgata tatcttatta actttgataa agattttagt ggtgatctta
     1861 ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat cggtggcggc
     1921 aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt ttttcattga
     1981 taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca gaacatgaca
     2041 aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt aaaagaaaag
     2101 aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat cagcgcttta
     2161 tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat tatatgataa
     2221 aagcaattca aacccatggt gcactaaaag ggacggtaat ggggactgca cggatcttac
     2281 gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc caattaacgc
     2341 gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat acgagtagca
     2401 ctgagcgata aagaaaaaga ggaaaattat tgtcaaagta acaactccgc aactacttat
     2461 atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg tccttgttat
     2521 tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg agtggctgga
     2581 tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg gaaaaagaat
     2641 acgaaatcgt caatgtagca gtagccccaa cgtatcaagg aaaggggatt ggcggaaaat
     2701 tacttgagac agcttttcag aagctttcac aactcgcttc cagtcaaact cagatcatca
     2761 tacgaaccgg cagtactagc agtgcggcgt tacatctcta tcaaaaaatg ggctatgtgg
     2821 agattggtcg agtcaaagac tattttatcc atcattactc tgagcccatt tttgaaaatg
     2881 gagatcaatt aagggatcaa gtaattttag caattcactt acctaaaaat agctttaact
     2941 aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt acaatagctt
     3001 tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt ggtggatcgg
     3061 ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa tttgcaacgc
     3121 ctgaatcttg ggaagaaatg aaagcgcaat tatttcaagt ttataaaaaa atactcgttt
     3181 cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat gagaaacagg
     3241 tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc cgagatttgg
     3301 aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc ggaatggctg
     3361 aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg gcaggaactg
     3421 ggattggcgg ggcgttgttc acgaaaggac aaatcaataa aggggcacat ctttatggtg
     3481 gagaatttgg gttgatgttc ttaaatggcg ataagacatt cagtaaactt ggtacagcgg
     3541 tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaattct tataccggaa
     3601 aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa gttgagagtt
     3661 tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat ccagaagtga
     3721 tcgtattagg cggcggtgtt tctgcaaaag aagggctcat tgatgagatc aaaagccgaa
     3781 tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt ttattatgtg
     3841 aatacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct gtacgtcaag
     3901 gggagaatta agagctttag agaaggtgca gcttgcataa aagaagtgaa tgaaatgaat
     3961 aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata tctgagaggt
     4021 gaaaatgatg tcaaaaaaaa ataaagatgt tgaagtgcgt atagaagaag tgaaaaaaga
     4081 tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa tgattggtga
     4141 gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag attttggaaa
     4201 agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc tgcacgaata
     4261 aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat taggtattat
     4321 aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa acgcgacgga
     4381 ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat ttcattgggg
     4441 tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc gaatccagct
     4501 accccagtat accacaatcg taagctctcg ttagttcgag agctcttttt tttgctttgc
     4561 taccttgttc cttgaagatt ctgctgattt aagatagagt taagttaact acaagaattg
     4621 gagggatcca catgaaatgg aaagaaaaag tagacgaagc agctgaaaag ttatatgatt
     4681 tggtcaaatc agaaaaatac aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg
     4741 tccaggtaaa atcaaaacgc ccaacgaatc atacaaaaaa atggatggct aaaaaccgct
     4801 aataataaca aaagatccat aagtcggcat tttcatcgat tttagggtct tttgttattt
     4861 tctaggttga acatatctaa aaaattgttt ctgacaatag gagtaggaag aacgatgtgt
     4921 gtctaaatcc tctgttgatg gatagaaacc tttatctttt tcagttgtga atcaggtata
     4981 taccagttat aatagaagaa acgaaaggac gggtgtacgt tatgtcgaat caattacctg
     5041 tatatataca gattcatgac caattaaaaa atgaaatcga acaaggtata tggaaagtcg
     5101 gagaccgtct tccttcagaa agagaattag cagtgaaatt caatgtcagc cgtatgacgt
     5161 tgagacaagc aattcaaaac ttggcagatg aaggaatctt agaaagaaag atcggttcgg
     5221 gtacatatgt tgcgcgagaa aaagtacaag aaaaaatgtc tggtgcaaca agttttacag
     5281 aaattatgga atcgcaaaat cgagtaccgt ctagtcggac aatctcttat ttcttgactt
     5341 cgccaagctc aagtgaaatg gaaaaactaa acttgagtaa agatgacacg atcttgcgaa
     5401 tggaacgtat tcgtttcgca gatgatgtcc ctatttgttt tgaagttgcc agtgtgccac
     5461 aaaagctgat cgatggctac agcaaagcag agattactga atcgttgtat cgcacgctag
     5521 aagaaaaagg cgggaaaaag atcggtccag cgaatcaaac gattactgct gtgttggctt
     5581 ctgagaaaat tgctgactat ttagaggtaa aacgaggaga tgcagttctt cgtttgcgcc
     5641 agatttctta ttttgaagac ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa
     5701 atcgttttga gttttatttg gaaaaataaa aaacaggtga tccctgattc cttacttaga
     5761 aataaaagag aatcagaatc acctgttttt atatatgatc agttaccagt tgctcgtgat
     5821 aagaaacagg accctcttca aattggactt gtccgttcag cagctcaatg atggattctt
     5881 taaagtggtc agcacttttt tcatctacag cacagatgac gctaacttga tcagtatatt
     5941 ggatttcttt tataaaaaaa tgttggagtt caagataatt ctgtaatttt cctagttgcg
     6001 gatacgtgat catcacagag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag
     6061 acaatgcttg agagacagca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg
     6121 ttcctccaaa atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga
     6181 cttccaacat cggtacgcca gccgttccac ttggttctcc atcatcgctg cttcgctgga
     6241 tttcattttt ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt
     6301 tttttatttc ttgaatgaaa ctttttgctt ggtcctctga ttctattcgt ctaagagaac
     6361 agataaagcg tgattttttg atctcaatct cactttgtcc attttctttt attgtacgat
     6421 agcgctcgat catcttgtca cctctttttt tcattttctt tattcagtat aaataaagtt
     6481 ctaggttgtt tcaacactgt aacggttgcg caatcgctgt agtatgcgat aataaagaca
     6541 aaggtaggtg gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta
     6601 tttgaagaac aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct
     6661 gtttcccagt aacggacttc ccaaaacagc aagggaaagt acagattggt gggcaaatga
     6721 ttataaacac cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt
     6781 catcaatttg gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt
     6841 tgattaaata aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag
     6901 taacagacgc gaagaaaacg tttgcgtagt ttgttttaaa aaggagagaa aagtatggct
     6961 aaaaaaacaa tcatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa
     7021 atgcaaaaag ctgctgaagc aaaaggaatc gactcggata ttttcgctgt gtccgcttct
     7081 gacgcagata acaatcttgc aaataaagat gtagatgtac tgttattagg acctcaagtt
     7141 cgctttatga aagctgattt tgaaaaacgt ctggaaccaa aaggcattcc tttagacgtc
     7201 atcaatatgg ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg
     7261 ctcataggtg aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg
     7321 cttgtttttt cgtatatttt tttagggcga ataatatgaa tcatttttgg ggtagaaggc
     7381 ttctgacgag agatattaaa gcaatagact gtgaggaggt aaatttacca aattatgaga
     7441 agatcgcagc agtggaacgt ttgggcaact ggattctttg tcatcgctgt ggaatgagga
     7501 cgtccgtcat tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt
     7561 tagggcgatg cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc
     7621 gagaagtcgt ctttttttgg acaggacagc ttactgaaaa acaaacggaa atcgcaaaga
     7681 gaatactttg ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga
     7741 aaacagagat gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga
     7801 tttgtacacc gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc
     7861 cagaagaaaa aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt
     7921 ttgttatctg tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga
     7981 tcgatgagat cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa
     8041 cagcgattaa accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac
     8101 taaaggaaat caagcagacg tttgacatcc acaaacttgc tttgcgcttt catcggcgtt
     8161 tacttcctga acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga
     8221 agaagataaa atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt
     8281 tattttgtcc aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc
     8341 cgagatatga gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga
     8401 aaatgcgaga agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca
     8461 catttgaaag aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt
     8521 ctttggtaca aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat
     8581 actttttcta tgaagaaaaa actgcagcta ttcgaaaagc ttgtcaggaa atcaaagaaa
     8641 tgaatgaaga agggaagaag atgctgtgag gtgcagttgc tgcagtaaag atatcgtcca
     8701 aaatttaaca ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc
     8761 agtttgtcgc gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc
     8821 aatccatttc aatctagaga gtacgaaaaa gaaagtaaag tctattcccg tttactgcag
     8881 tgactgtcaa gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt
     8941 tgtgtacgat gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg
     9001 actagcaggc acctttgcat ataaatggac gcagataaaa aaagaatacc cgacatacat
     9061 ctattgtcca atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag
     9121 cgagatgttg aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct
     9181 gctggctcaa gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact
     9241 tgcagtcacc ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac
     9301 aacaggacag actcttttcc acgcagcaga ttgtttgcag gtcgcacagc cgaaaatcat
     9361 ccgaaccatt tctttagcaa gatgaattag atgtggaaat ttgatagcga tttaatttgc
     9421 aaataaccta gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt
     9481 cttgattgtg gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa
     9541 atatcgaagt aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac
     9601 gctatttcag tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa
     9661 aaacagcaaa agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa
     9721 cttctccaga tttatacgca agtatcgatt tggttgttga caaattggaa cgccaaatcc
     9781 gcaaattcaa aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcagatg
     9841 ctactgcttt attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac
     9901 gtacgaaacg cctttcattg aaaccaatgg atagtgaaga agcagtctta caaatgaaca
     9961 tgctgggaca taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt
    10021 accgccgtaa agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat
    10081 aacaa
//
LOCUS       DAFCNJ010000078         9362 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000078 DAFCNJ010000000
VERSION     DAFCNJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.082"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(93..761)
                     /locus_tag="LNE35_002509"
     CDS             complement(93..761)
                     /locus_tag="LNE35_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6911612.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="LNE35_002510"
     CDS             895..1182
                     /locus_tag="LNE35_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911613.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="LNE35_002511"
     CDS             1598..3136
                     /locus_tag="LNE35_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBK6911614.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="LNE35_002512"
     CDS             3133..4098
                     /locus_tag="LNE35_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6911615.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="LNE35_002513"
     CDS             4085..5407
                     /locus_tag="LNE35_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6911616.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="LNE35_002514"
     CDS             5648..6391
                     /locus_tag="LNE35_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6911617.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="LNE35_002515"
     CDS             6406..7398
                     /locus_tag="LNE35_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6911618.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="LNE35_002516"
     CDS             7410..8147
                     /locus_tag="LNE35_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6911619.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="LNE35_002517"
     CDS             8137..8859
                     /locus_tag="LNE35_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK6911620.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9362)
                     /locus_tag="LNE35_002518"
     CDS             complement(8943..>9362)
                     /locus_tag="LNE35_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBK6911621.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3069 a         1607 c         1972 g         2714 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
     9361 ta
//
LOCUS       DAFCNJ010000079         9357 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000079 DAFCNJ010000000
VERSION     DAFCNJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.083"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="LNE35_002519"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="LNE35_002519"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBK6911622.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="LNE35_002520"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="LNE35_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBK6911623.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="LNE35_002521"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="LNE35_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBK6911624.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="LNE35_002522"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="LNE35_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBK6911625.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="LNE35_002523"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="LNE35_002523"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBK6911626.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="LNE35_002524"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="LNE35_002524"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBK6911627.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="LNE35_002525"
     CDS             complement(8053..8523)
                     /locus_tag="LNE35_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBK6911628.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="LNE35_002526"
     CDS             complement(8569..8994)
                     /locus_tag="LNE35_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBK6911629.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="LNE35_002527"
     CDS             complement(9061..9330)
                     /locus_tag="LNE35_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBK6911630.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         2355 a         2095 c         1621 g         3286 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttt
//
LOCUS       DAFCNJ010000080         9280 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000080 DAFCNJ010000000
VERSION     DAFCNJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.084"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..435
                     /locus_tag="LNE35_002528"
     CDS             <1..435
                     /locus_tag="LNE35_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBK6911631.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="LNE35_002529"
     tRNA            complement(759..842)
                     /locus_tag="LNE35_002529"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="LNE35_002530"
     CDS             1370..1870
                     /locus_tag="LNE35_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6911632.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="LNE35_002531"
     CDS             complement(2060..2404)
                     /locus_tag="LNE35_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBK6911633.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="LNE35_002532"
     CDS             complement(2558..3478)
                     /locus_tag="LNE35_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6911634.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="LNE35_002533"
     CDS             3665..4156
                     /locus_tag="LNE35_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBK6911635.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="LNE35_002534"
     CDS             complement(4180..6555)
                     /locus_tag="LNE35_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBK6911636.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="LNE35_002535"
     CDS             6881..7507
                     /locus_tag="LNE35_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6911637.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="LNE35_002536"
     CDS             7530..8234
                     /locus_tag="LNE35_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6911638.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="LNE35_002537"
     CDS             8426..9259
                     /locus_tag="LNE35_002537"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBK6911639.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3010 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct
//
LOCUS       DAFCNJ010000081         8675 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000081 DAFCNJ010000000
VERSION     DAFCNJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.085"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1101
                     /locus_tag="LNE35_002538"
     CDS             <1..1101
                     /locus_tag="LNE35_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6911640.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="LNE35_002539"
     CDS             complement(1280..1609)
                     /locus_tag="LNE35_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK6911641.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="LNE35_002540"
     CDS             complement(1599..1871)
                     /locus_tag="LNE35_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBK6911642.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="LNE35_002541"
     CDS             complement(2322..3125)
                     /locus_tag="LNE35_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK6911643.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="LNE35_002542"
     CDS             complement(3103..4227)
                     /locus_tag="LNE35_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6911644.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="LNE35_002543"
     CDS             complement(4243..5058)
                     /locus_tag="LNE35_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6911645.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="LNE35_002544"
     CDS             complement(5048..5920)
                     /locus_tag="LNE35_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6911646.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="LNE35_002545"
     CDS             complement(6005..7294)
                     /locus_tag="LNE35_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6911647.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7522..8166)
                     /locus_tag="LNE35_002546"
                     /pseudo
     CDS             complement(7522..8166)
                     /locus_tag="LNE35_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAFCNJ010000082         8586 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000082 DAFCNJ010000000
VERSION     DAFCNJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.086"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1511)
                     /locus_tag="LNE35_002547"
     CDS             complement(45..1511)
                     /locus_tag="LNE35_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBK6911648.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="LNE35_002548"
     CDS             1898..2494
                     /locus_tag="LNE35_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK6911649.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="LNE35_002549"
     CDS             complement(2552..3034)
                     /locus_tag="LNE35_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911650.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="LNE35_002550"
     CDS             complement(3140..3337)
                     /locus_tag="LNE35_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911651.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="LNE35_002551"
     CDS             3632..4390
                     /locus_tag="LNE35_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBK6911652.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="LNE35_002552"
     CDS             4450..4878
                     /locus_tag="LNE35_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911653.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="LNE35_002553"
     tRNA            4924..4996
                     /locus_tag="LNE35_002553"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="LNE35_002554"
     CDS             complement(5146..5517)
                     /locus_tag="LNE35_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6911654.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="LNE35_002555"
     CDS             5732..5917
                     /locus_tag="LNE35_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911655.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="LNE35_002556"
     CDS             complement(6000..6188)
                     /locus_tag="LNE35_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911656.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="LNE35_002557"
     CDS             complement(6212..6475)
                     /locus_tag="LNE35_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911657.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="LNE35_002558"
     CDS             6633..6890
                     /locus_tag="LNE35_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBK6911658.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="LNE35_002559"
     CDS             complement(6961..7152)
                     /locus_tag="LNE35_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911659.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="LNE35_002560"
     CDS             complement(7293..7613)
                     /locus_tag="LNE35_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911660.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="LNE35_002561"
     CDS             complement(7636..8070)
                     /locus_tag="LNE35_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6911661.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..>8586
                     /locus_tag="LNE35_002562"
     CDS             8405..>8586
                     /locus_tag="LNE35_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6911662.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2718 a         1694 c         1362 g         2812 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattc
//
LOCUS       DAFCNJ010000083         8493 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000083 DAFCNJ010000000
VERSION     DAFCNJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.087"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..173)
                     /locus_tag="LNE35_002563"
     CDS             complement(<1..173)
                     /locus_tag="LNE35_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6911663.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="LNE35_002564"
     CDS             complement(593..1714)
                     /locus_tag="LNE35_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBK6911664.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="LNE35_002565"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="LNE35_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBK6911665.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="LNE35_002566"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="LNE35_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBK6911666.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="LNE35_002567"
     CDS             complement(4304..5215)
                     /locus_tag="LNE35_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6911667.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="LNE35_002568"
     CDS             complement(5232..6239)
                     /locus_tag="LNE35_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK6911668.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="LNE35_002569"
     CDS             complement(6236..8365)
                     /locus_tag="LNE35_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6911669.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8493)
                     /locus_tag="LNE35_002570"
     CDS             complement(8370..>8493)
                     /locus_tag="LNE35_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6911670.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DAFCNJ010000084         8273 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000084 DAFCNJ010000000
VERSION     DAFCNJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.088"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            296..829
                     /locus_tag="LNE35_002571"
     CDS             296..829
                     /locus_tag="LNE35_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911671.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(955..1632)
                     /gene="nth"
                     /locus_tag="LNE35_002572"
     CDS             complement(955..1632)
                     /gene="nth"
                     /locus_tag="LNE35_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBK6911672.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1639..2337)
                     /locus_tag="LNE35_002573"
     CDS             complement(1639..2337)
                     /locus_tag="LNE35_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK6911673.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2605..3789
                     /locus_tag="LNE35_002574"
     CDS             2605..3789
                     /locus_tag="LNE35_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBK6911674.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3795..4238)
                     /locus_tag="LNE35_002575"
     CDS             complement(3795..4238)
                     /locus_tag="LNE35_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6911675.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4464..4751)
                     /locus_tag="LNE35_002576"
     CDS             complement(4464..4751)
                     /locus_tag="LNE35_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911676.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4922..6274)
                     /locus_tag="LNE35_002577"
     CDS             complement(4922..6274)
                     /locus_tag="LNE35_002577"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBK6911677.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6547..6993)
                     /locus_tag="LNE35_002578"
     CDS             complement(6547..6993)
                     /locus_tag="LNE35_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6911678.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(7229..8155)
                     /gene="cysK"
                     /locus_tag="LNE35_002579"
     CDS             complement(7229..8155)
                     /gene="cysK"
                     /locus_tag="LNE35_002579"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBK6911679.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2277 a         1656 c         1320 g         3020 t
ORIGIN      
        1 aaaaaagaaa ccacgtctac aagcgtggtt tcttttttgt gtctatgtaa aaaacagcta
       61 caaataaaaa tcatgtacca agctgtatta catgattttt tataagacta tccagcatat
      121 acatgtgtga tctaacattt agcttaaaaa ttagaaaatc catttttctt ttatcattac
      181 atatacttta tccatttact tgtttataat taaatcggct aaatacacac tagtctttta
      241 tttagtgcca cttcttaata atcccattgt tattatcaaa taaaaaatac aatatatggt
      301 tttgaataat tttttgaaag aaggcttcat tttgagttat ctgatagtat gcttaattat
      361 cataatttgt ctgcaccatc tttttttatc gaaaacaata cctcgttatt ccaagaataa
      421 aaaagctgaa aaattttgtt tattattaaa taaatttact ttaattgctc ctatccttgc
      481 atttattatt ttttctgtac ttttaagcac tactcttaaa ggaaaattta tggaaagaag
      541 ctctcatgca atgattctca cttttttatg gttgttgttc accagaatct atatttttct
      601 gatgagcctg aaaccgccca aatccattag tttatgttta gttataaatg gtattttttt
      661 attatcactt atcattttta ttacaccttt agatagatac gtcacttatt tatataatcc
      721 tttggaatac tggacttatt ttataggtat attggaaggc attattttct acatcgggta
      781 ttttcccaat aaaaataaca acttttattt ttatcgcaat aaattgtaat aagttaccat
      841 cctagctact gagtatgtac atttactttt tcatgcaaaa aagataaacg aaacagcttt
      901 tctccataat aacaagataa ttcatacatt tatttgcaat taaagaaacc acgtctacag
      961 gcgtggtttc ttttttagcg ccttttcttt tagacgcatc cgatttttcc catcttgaca
     1021 tattgatagc aaaggacata cctcgcactt gggatttctt gccgtgcaat gatatctacc
     1081 aaaaaaaatc agtgtatgat gtgtctttac ccataattct tgtggtactt tccgcatcag
     1141 tgtttcttct acttccatga ccgtagcatc tagtttgcag atcctcaggc gttttgagac
     1201 acgttccacg tgggtatcta ctgcgattgc tggtatcccg aatgcatccc ccaatacgac
     1261 atttgctgtc tttctgccta caccaggaag agacatcaat tcttctcttg acgttggcac
     1321 ttgcccatcg aaacgttcaa tcagctgctg tgcacacgct ttgatgtttt ttgctttatt
     1381 tcgatacaaa ccaatcgttt ttattttaag aatgatttca tcgattgaag cttctgctaa
     1441 cgcatcgggt gtcggaaaag aggcaaacaa atctggcgtt gctttattca ctgatacatc
     1501 tgtcgcctgg gcacttaaga tcactgcaat caataactca aatggattat tgtgcttgag
     1561 ttctccatgt gcttcgggaa acattccata catcgtttct agagcttcca ttgttttttg
     1621 tttgcttaac atattccgct actcctccaa ccaatttttc agtgagactt tcggcaattc
     1681 ttcttgttga gctgactgtt ctacttctct ttgaagaagc tgctgttttc ttcgtttttg
     1741 gtcttcttcc acttgttgtt ttgtacgtag attttttctt tcccacgata gcaaaatgcg
     1801 gtcaacgtaa ttaaaactat aagcctgatt taatacggct tcccgcagtg ctagcttcaa
     1861 aatttccggc tgataatggt cttcttctag ccattgtccg atccgctgga actcgatcga
     1921 agaaagcggg cgtccaaatt cttgttcaaa taattgatac actgactgga tttctttttc
     1981 ttgtgttttt tcttcgtatt ttttttcttg tgtttttaaa tactcaccta atagatcata
     2041 gatggggtaa agattatatc gatctgcttt tttaccgttg acaatcgtcg tttctatctt
     2101 gataaatcca tttgttacga gacgatccag tatttgaaaa atctgatctt gtttgattcc
     2161 catatccata gcaatcatct gcaaatcagg gaaagtatct ccttcaagct gtgccatatg
     2221 caactgtaga acaaataaaa attcttcaga ttgtagaccg atcctatgat aatttttaag
     2281 taaaagatta gatatgactg tttgtccagc gcttaagtaa ttttctaaat tcaacatgat
     2341 ttctcctccg ctcaatcaca gtatacgtaa aaaaaacatg ggaagcaaga acaggtttct
     2401 tttctattta cttcactgat cggtcagatt tacaatgtcg tctacttgat ttttgtttgt
     2461 tcatgttttt gatgcgtatc ttcacttttc ggaataaata aattaagtag atttcgctgt
     2521 tccttagaat gttgacaagg aaacaaaatt actatacact atttttgttt ccaagttaac
     2581 attattgcat aaaggagttt ttacatgtac agtttcacaa ataaacattc taaagttttt
     2641 ggatacgtct ctgtcttatt cactggatta ggatttacta tcgtcagtcc tgtacttccg
     2701 tttttaacgc tgccttattc tcactccata catcagcaag ctttctatat cactttgctc
     2761 atgtctgttt atgctctagc tgccttctta tctgccccca ttctcggtag cttaagtgat
     2821 cattttgggc gtcatccgat ccttattatc agtttattag gatcaagtat cggatatttg
     2881 ctttttggtt taggaaattc gatatggatg ttgttccttg gtcgtatcat tgaaggattg
     2941 acggcaggag aaatctccac cttatatgct tactttgccg acatcacgga acctaatgaa
     3001 agaaccaaag tttttggatg gatgggtgct ttagtcggta taggaacaac gctgggtcct
     3061 attatagggg gtctattagc tgagttgggg aattctgttc ctatatttat tggagcactc
     3121 tttactttct taaatgccgt ttatggctac acatttatgc tagaaagcct tccaataaaa
     3181 aaacgttcag tagatttaag tttctcccat gtaagacctt ttcaccaatt acaacaatta
     3241 ttcaagattt catccgttat tcctttattg acagcaggtt ttgctgtttg gttggcagct
     3301 ggctctcttc aatctatctt ttctcaattt tcgattgacg cctttcagtg gaaggctggg
     3361 ttggttggtt taagcttctc tttgattgga atcttggata ctgtctcgca attgttcatt
     3421 atgcctcgat tgctcaaaaa attttcagaa cagcagatca caagaatagg gatgttcagt
     3481 gaaatcctcg cctatctatt catcacatta tctggaattc ttctactgcc gattttgttt
     3541 ttatttggga ttatctgtta tggtttcggt gattctattt ttactcctgt attcaatggc
     3601 caactatcca attctgtctc agataaccag caaggtcttg taatgggcgg tacccaaagt
     3661 atacaatctc tatcgcgcgt catcggcccg ctaatcgcag gacagcttta tgctgcagca
     3721 ccatttcttc cgacggtatt cggatttttg cttatgattt ttgcctatta tcactacaat
     3781 aaaaaatagt agcttcattt gctacttcgt ataaattctc tgtatcgacc gataaaacga
     3841 aatactgtgt caaattcttc ttttgacata ttttcaaata cagtagcatc tcgctgtacc
     3901 catttttgat ggagttgctc gtgtctattc cttatttttt gtccgattgg cgtcaattga
     3961 aaatagattt cttttttatt ctcttcgctt tgataagtgt ttatgatccc tctagctatc
     4021 agtttttttg ttagcttact gatggctcct ctagtcatgt aactagcatt cgctaatttt
     4081 gtcacgttag gatcagggat tttcccgata tgttcaatca cttctagttc atttagagta
     4141 aatcctgcaa attctttttc catttctggt ttattacgat gaataatctc attagctaaa
     4201 tccagatatt cttctattag cagttgtctt ttatccattt ctacaccttc tttttctttc
     4261 attatattgt ttttttgttt cctattcaac aaagataaaa atattttgta ggaaaaccca
     4321 atctgaaacc aagaaaaaaa ggggttgaca aagcttgtca taaccccttt ttctgattaa
     4381 aagatagtaa agagacagtc atcggtaatt gtgatgattt tttgtcatca cttaaggatg
     4441 agcggccttt tcaaaaactg tctttaaaga tcatcatcag tttctagcac atatggctga
     4501 ccttcatatg tgtactctgt attcaatgtg ccgtcatttt catctgagta gatgcgtcta
     4561 ctgaaaggaa aacgatgaga aacattatct ttggcgtcat ttgctggcat cgtgtaatat
     4621 tcctcttgcg tttcattcaa ctgagagagt acttgatctg ccaactcatc aatattgatc
     4681 gttaatttct tcgaagcact tttataatct tctaaaaatg catcttgttg gacaaaatat
     4741 tctttattca tcttctttct cacctttctt ttcaaaatca aataaagctg cttgttcttt
     4801 tatgtaacag ctttatcttt gctgcttatt gtgacaaaaa attcgaagaa agacaactaa
     4861 ttgtactttt cacaaaaaaa gacagagggt cgtgacaaaa ttgtgtcaca accctttttc
     4921 tttattgtgc agtttcgatt tcgcctagcg ctgcttgtgc aagtaggttc aagtaattcc
     4981 atgggcggtc aaaatgtggt tggaagaaga aatctgaaag tgcgagatct tctacagtca
     5041 ttttattttg aacagctaaa gacatggtat ttgctgattg tgtaatgtcg tatttggaca
     5101 tgaactgagc acctacgata cgattcgttc ctttttcata gactagctcc atcattactt
     5161 tttcagtagt tggcatgaac tctggacgat aattatcttc aaatacagtc gcttggactt
     5221 ctagatcgtt caaagttgcg ctttctttgg tcacacctgt cgaaccgatc gtccagccaa
     5281 ataggtaaag tcctgaagtt ccttgagtac cacgatacgc cattttatga tctgtcaagt
     5341 tatttcccac aagcaaacct tgacgaacgg catttgttgc caaaggaata tagttttgcg
     5401 tgccacttgg attgtagttg actactgcag agtctccagc tgcaaaaatg tctggattac
     5461 ttgtctgcat gtattcattc actttgattg ccccgtttgg cagtgtatct acttttcctt
     5521 cgatcagttt tgtatttgga cggaagccca cacataaaat gaccatatct gtatcaaatg
     5581 tatctgttgg tgtagctact tgtttcactt ttccttcttc atctgtgata aattcagtaa
     5641 cattttcgcc tagcgcaaga gtaacaccgc ggtctgtcaa ttctttttct aagatatctg
     5701 tgaatggttt atctagatat ttattcaaga tgcgatctaa tccatcaatc aatgtcactt
     5761 gttttccaga ttcgacaaaa gcttctacta attcaatacc gatatatcca ccaccaacaa
     5821 ttactacacg ttttgcttct ttcgcttgtg caataatttc atttgcttga ttgtagtttt
     5881 tgcataggag tacatttttc gcatcgattc cactaatcgg tgggataata ggccatgaac
     5941 cagttgtcat cactaattta tcataagaaa ccgtttctgt ttctcctgtt ttcagatctt
     6001 ttgccgttac tgttttattt tctgtatcga cgtctgttac atcgtgttcc attttaactg
     6061 ttgctcctaa tgaagataat tcttcaggat ttgagtagaa caagcctgct gggtccttta
     6121 ctactccgcc gacataaagt gcgataccgc aagataagaa agaaatattg tcatttcttt
     6181 catatactgt cacttctgct tctggatgat tttttaaaat actttttaca gctgcagtac
     6241 ctgcatgagt acagccaatt actactactt tcatttatgg tccctccgat taacattttg
     6301 tccttcgaca aaactactat aacagaaata aaacggatac aacagtttaa tgcttgtgaa
     6361 ttcacaaagg tttgtttgta gtttcattcc ttttcaagca taagaaccca atatctacaa
     6421 gattcttatt ttaataatat tcacttttat atctaagatt gtgaataaaa gacaaaaaaa
     6481 taaaaaagct agcgtttttt ttacacagct agcttttttt aggtataaca cttgtagaaa
     6541 ctgtttttac atttgcgcct tttttgctga ctctttacaa tccggacaga gtccatacaa
     6601 ttccaaccga tgttcattaa tctcaaaatc agtcagtctg cttgcagcca tttctatatc
     6661 ttcaagccct gggtaatgaa aatcaacaat cttaccgcag ttttcacaaa ttacatgata
     6721 atgacgcttt gaactgaaat caaatcggct agaagcatcg ccgtacttca tttcttgaac
     6781 aaatccaatc gaagtaaata agcgaaggtt attgtaaaca gtcgcaacac tcatatttgg
     6841 aaaccgatgt tcaagtgctt tataaatctc atcagccgtt ggatgggaat gatgttcaat
     6901 caaatattct aaaaccgcgt accgttgagg tgtgattcga atgttctctt tttttaactt
     6961 ttcgataacc tcttcgacta acttattttc catgtaaaca ccccacgttg ttattctcgt
     7021 cttatcatac tatttttaca ttttaaaatc cacttaacat gaaaagaaaa ttcaatttta
     7081 ttgtatttta atgaaaaaag acgtaaaaca gtcgcgttca ctattctacg tctctattca
     7141 aataattttt ctcatcataa atttttgtgt gttcttttca tctttaaaag ttttttacat
     7201 aaacctatct ggagaaaaac caagtgcctt atatctcttg gtaaagggct gtagataaat
     7261 aacgttcacc attgtcggga accacagcta ataccttctt gccttttcct aattcttttg
     7321 cgacttttag cgcggcagca attgcagcgc cagatgagat gcctactaaa atcccttctt
     7381 ttcgcccaac ttctctagct gtttccatcg cttcgtctcc actaatagaa aggactttat
     7441 catacacaga agtatccagt gttttaggaa caaaacctgt accgattcct tggattttgt
     7501 gaggtcccgc ttctttccct tctaaaatag cagattctgc tggttctacc ccgattaatt
     7561 cgattttcgg atacacacgt tttaattctc ggccagcgcc agtgatcgtt cctcctgtac
     7621 cgatacctgc aacaaacgcg tccaagccat ttactccaaa tgcttgatgt atctcaggac
     7681 cagtcgtctt ctcatgcaca agaggatttg cttcattttc aaattgtaat ggtaaaaagt
     7741 agccgttttc ttttgccaaa cgttctgctt ctctgattga tccagaaata ccatccgcac
     7801 caggagtcaa aatcaactct gcgccgtacc ctttcatcaa taaacgtctt tcgatactca
     7861 tcgtctcagg catcacgatg attactttat atccttttgc aactcctacc atcgacaatc
     7921 cgattcctgt atttccagaa gtaggttcaa tgatagtatc acctggtttc agtagaccat
     7981 cgtgttctgc tttttctatc atacttaaag caatacgatc ttttacactt cctcctggat
     8041 taaaaaactc aagctttaca aaaacatctg ctgaatcttc tggtacgatt ttatttaatt
     8101 tgacgattgg cgttttgcca atcaattcgg tgactgagtt atagatagct gtcatcttca
     8161 attcctcctt gtctttgctc ttcttactta tcataataca atctgttcat ttatggcgag
     8221 tgattcgtct ttgactagca aaaaaaagac aaagatatac catatatggt aat
//
LOCUS       DAFCNJ010000085         7979 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000085 DAFCNJ010000000
VERSION     DAFCNJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.090"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(771..2117)
                     /gene="gor"
                     /locus_tag="LNE35_002580"
     CDS             complement(771..2117)
                     /gene="gor"
                     /locus_tag="LNE35_002580"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBK6911680.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2251..3504)
                     /locus_tag="LNE35_002581"
     CDS             complement(2251..3504)
                     /locus_tag="LNE35_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK6911681.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3510..4433)
                     /locus_tag="LNE35_002582"
     CDS             complement(3510..4433)
                     /locus_tag="LNE35_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK6911682.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4654..7146)
                     /locus_tag="LNE35_002583"
     CDS             complement(4654..7146)
                     /locus_tag="LNE35_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBK6911683.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7153..7620)
                     /locus_tag="LNE35_002584"
     CDS             complement(7153..7620)
                     /locus_tag="LNE35_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBK6911684.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7756..7829)
                     /locus_tag="LNE35_002585"
     tRNA            complement(7756..7829)
                     /locus_tag="LNE35_002585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7793..7795),aa:Arg,seq:acg)
     gene            complement(7887..7960)
                     /locus_tag="LNE35_002586"
     tRNA            complement(7887..7960)
                     /locus_tag="LNE35_002586"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7924..7926),aa:Pro,seq:tgg)
BASE COUNT         2230 a         1722 c         1289 g         2738 t
ORIGIN      
        1 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg
       61 agaaactggc ttttgaacac cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt
      121 attaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc
      181 gagaaactgg cttttgaaca ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag
      241 tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg agctaatacc
      301 gaaggacagc tgattgaaca ctatttgttc ggaaaaagtg actaactatg acacaatttt
      361 tttcacagtc ccttttttca cttgatttga ttcgtaccag cgaacttgac gagaactttc
      421 ttggtttggt aactatatgg tattctttta acatcctcgt ttgcttcttc taatttattt
      481 atacaaagtc acactttttt aaacatctgt tcttcgatac atttctattt cttttaaaca
      541 tcgataccaa tattttgaac ttaagagtgc ataccaaatg gattcattta ctcttcgtca
      601 gttacgaaga taatcaaaat ctccaacttc cctacttatt tcgttttgag aattatcgaa
      661 gcaatcttac ataaataact aaaaaacaaa aaaccagaaa atcagtatac tgattttctg
      721 gttttacatg gtttgttcaa taaaaagctg ttggaactgt taatattaaa ttatcgcatc
      781 gttacgaatt cttccgctcc tgttggatgg atggctactg tattgtcaaa gtcttcttta
      841 gttgcaccca tcttgatagc cacggcaaaa ccttgcagca tctcatcgac accaacacca
      901 atagcatgta agccgacgat tctttcttct tttccgacac agatcaattt catttcgcat
      961 ttttggcgat aatcatttaa tgcaaagtac attggggtaa agcgtgaacg ataaactttc
     1021 aattcatttt cgccatattt ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt
     1081 ggtggatgcg taaagacgac tgtcggaaca agtttatagt caagataaga atctttcttt
     1141 ccattaaata aacgttctga taaacgtcta ccagctgcga ttgcaacagg agtcagatct
     1201 aatttaccga tcacatcacc aacagcaaaa atatgttcat ccgttgtatt ttggtattta
     1261 tctactttga taaacccttt tttgtccaat tccacatttg tattttccag acctaatttt
     1321 tcggtattcg gtacacgtcc accggcaaaa agtacacaat caccagttaa ggtttcacca
     1381 ttttcaaata gcaccgtata ttcgtcattg tttttagtaa tttctttagc aatatgatgt
     1441 gcgtaagttt ttattccagc ttcttggtac atttggatca aattgtccga aagcatttta
     1501 tcaaacgttc taagtggtcg ttcttttcga tatgcccaca tgacttcact acctaaacca
     1561 cgaaatacac ctgaaagttc agccgcaatg tatccagcac ctaaaacgat tgctctctta
     1621 ggtaactctg ttaattcaaa aaatccattt gaatcaattg cgtattctcc accaggaatg
     1681 gtcattttac ttgcacggcc tccagtagcg atcaagattt taggagcacg ataggtttca
     1741 ccgtttactt cgatcgtttg actatcgata aaagtagcgt aaccttttat agcctcgaca
     1801 tgattgctat ctaatcctct ttgataagca ccgtgtagaa aatcaatata tttttctcgt
     1861 ctttctacta gaccggcgaa atcaacattt tttaaatctg cttggatacc gtaacttgag
     1921 gcatctcgtt caattgtttc caaaatcgta cttgcttgcc acataacttt tttaggtaca
     1981 caaccgacgt tgacacatgt tccgcccaat tcattcgctt caatcagcaa aacttttgcg
     2041 ccatgcatcc ctgctctgtt tgctgaagca ataccgccag agccgccacc aattacgatg
     2101 taatcatata tttgcatttt gttcctcctt attgaagaga tcaatcaaaa cctttattta
     2161 acgatccttt ttctacagaa aacaagtgta acacttttct tgaattttgt atgcgaagat
     2221 gcttaaaaca gggatatatc agctttttcc ttatttacgt tttcggatca tatctccagg
     2281 tttcaccggc tgtgcgactg gcatagttaa aatcatcatt ggatttggtg ctcggtcaat
     2341 ggattcgcct tcttcattat gcataacttc aacggtttga ctgaaatgac tgaatcctgg
     2401 accataaaat tcgatttcat ctcctacaga aaacaagttt ctctgacgta tcgtagcaat
     2461 ctgtgttgcc tcatcataag ataatacttc accgacaaat ttatatactg ggattttacg
     2521 acgttcaccg aataattgtt cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa
     2581 ttctcgttgt gctactttcc ataactcgtc gatccattct tgcttgcaga catagttttc
     2641 aggatcttcc atataactgt ctacagccgc tttatagaca tttgacaccg tagatacata
     2701 atggatcgat ttcattcttc cttcgatctt gaagctgtct acgccgtttt caatcaattc
     2761 tggaatatgt tcgatcattg acatatccac tgcactcatc gagaattctt cttccacttc
     2821 gcctgtatca gttaaactgt ttcgttcttg accaaaaggc atatcgaaca atccatactt
     2881 ccagcgacaa gattgagaac atccgccacg gtttgcatcc cgcatcgaca tatggttgga
     2941 caatgtgcat cgaccagaat aagagataca catggcacca tggataaatg cttcgatttc
     3001 ggtatccgta tttttacgaa tctctgccac ttcctccatt gaaacttcac gagctaaaac
     3061 gacacgttcc aacccttctt ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc
     3121 ttgagtggat aaatggatcg gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc
     3181 tggatcagaa acaatcactg cagaaatacc gacatcacgc aaactgcgga aaaattcccc
     3241 tgctccatct tcatttcctt catgggtgac catattagca gcaacgtata ccttcgcatc
     3301 atgttctttt gcaaaagcaa ctccttcagc catttcttca taagtaaaat tacctgcacg
     3361 gcttcttaaa ccatatgcat tcccgccaat atatactgca tctgctccat aatggatcgc
     3421 ggtttttaac ttttccagtg ttcctgctgg cgctaggatt tctggtcgtt tcaattttct
     3481 ttttactgtc atttctttca tcatactcct tatttgatag cagaaggatc aatataaaag
     3541 aatcctgtat ctaattcacg gttttcagga tgcaatttac tgatttcgct agtcattttt
     3601 tccgcttttt cttgatccca gctaccttct aaaatttgtt tttttgcttc ggcgaatagt
     3661 cgagtgatgt ttacgaattt ttcgcctgga gtaaataatc cgtccaattt ccatgtacag
     3721 taatgatgct gataaagttc attcaattca ttcgagagat tcagatcatt gctggcaaaa
     3781 atatgtgtgc catgactatc ttcataaata gaataatgtg tctcttcttt tttaggttcc
     3841 gagataaaaa gacctctttc acgatctttt tgttcgtctt gtttcgtata gttatagtaa
     3901 ttctgtagca gtggacgttt ggattgatgg atgcatgtag caccatacac aagggtttct
     3961 acaggaatat cgagtttttc ttccatcgcc ttcatctctt caaaaggaac ttcacgagca
     4021 agtactgctc caactgcgcc ttttttcgcc cagaaattga tttgacgtgc gctcgttact
     4081 aatgtttcag catcataaat atacggtata cgcaattcag gatttttttt catcgtataa
     4141 ataatacctg tatcaccaac tgtgatcaca tctacgttta tttcttttaa aaaagccaaa
     4201 tattcaggta catttttcat tttttcagga tgaatgatcc catttacagc aatattcgct
     4261 tttttaccat acttgtgtgc taattctacc aattgtcgct gttcttctcg tgtaaatgaa
     4321 gcaggcagac gcaatccaaa ttgctcttca ccgaaatata gtgtatctgc acctgcagca
     4381 agcaactgtt ctgcttgttc aatagtttct actgtggtta tgagttcgat cataattttc
     4441 tcctcctaca acttcataat agtagcatag ctctaaaaac tttccaatac ttcttctatt
     4501 taaagtcgga tttttgaaaa ttatttcaca aaaaatcaaa ataaagttcg atatattctg
     4561 tccaaatata aaaagagatt gtgacaaaaa tcttgtcaca acctcttaat caagtcttac
     4621 tattataaaa attcagctac acaggccaag taactatagg gaacctactg tttccttttt
     4681 aggtccgcga acattcaatg tgatcttacc ttttgaagca cctactgtta cgtggtcacc
     4741 taaacgaatt tctccgccaa gcaaagcctc gctcaatcga tcttcaattt ctttttgaag
     4801 tgcgcgacgg attggacgtg caccatattc aggatcaaat ccagctttcc cgatcacatc
     4861 gattgcagat ggtgtgattt tcacttgaat atcttgttct ttcaatcgtt tgataatcgc
     4921 tttgctcatg atttggacga ttgtatggat ttcatcttta ctcaatgaat ggaagacgac
     4981 agtctcatcg attcgattca agaattcagg tcggaaagct ttctttaact cttctaagat
     5041 acgtttttgc atggctttgt gatctttagt aacatcttgg acattgaatc cgacattttt
     5101 ttcttctcga atttgtgtcg ctccgatatt tgaagtcata atcatgattg tatttctaaa
     5161 gtccactttt ctacctttag aatctgttaa atgaccatca tccaatactt gcagaagtaa
     5221 attgaaaacg tctggatgtg ctttctccac ttcatctaag agaatcactg aatatggttt
     5281 ttgacgaatt ttttctgtca actgtccacc ttcatcatac cctacgtatc ctggaggaga
     5341 cccgattaat cggcttgtac tgtatttttc cataaattct gacatatcaa cacggatcag
     5401 ggcatcttca cttccaaaca tgacttctga aagtgctttg gccaattctg tttttccgac
     5461 accagttggt ccaaggaaca tgaatgaacc aattggacga tcaggatctt tcaatccgct
     5521 tcgtgcacgg cggatcgcac gtgaaactgc tttgacagcc tcatcttgac caacgacacg
     5581 ttcatgcaga agtccttcca gttctaacaa gcgttcgctt tcttttttct ccaactgctg
     5641 tagagggaca ccagtccact ccgatacaac tgtagcaaca tcttcttctg ttacacgatc
     5701 agaataacct tgctcctgtt ttgcttcaat tgcagaaact ttttgcagct ttttacgtag
     5761 tgctttttct tgtctgcgta attgagcagc attttcaaag tcttgttttt ggatcgctgc
     5821 ttccttttct tgtactagtt catcgatttc tagtttgatc acagttgatt ttgttaagtg
     5881 atcggtttga tccaaacgga ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc
     5941 gggtagttga cgagaagtga tataacgaac agaaagattg actgctgctc gtacagcttc
     6001 atctgtgatc tccactccat ggtgctcttc atatcttgta cgtaatcctt gaagaatcac
     6061 ttctgcttct tcaggagttg gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc
     6121 agaatctttt tcgatatatt tttgatattc atctagggtc gttgctccga tcgtttgtaa
     6181 ttctcctcga gctaacgccg gtttcaagat attcgaagca tcaattgctc cttctgctcc
     6241 acctgcacca attaaggtat gcaattcatc aatgaataag attacttgac catcattata
     6301 gatttcgtcg atgacctttt tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc
     6361 aaccaaagat cccatatcaa gcatcatcag gcgcttttct tgcatgtctt ctggcacttc
     6421 acctaaaatg attttttgag ctaacccttc agcaatggct gttttgccta caccaggttc
     6481 acccaccagt acagggttat tctttgtccg tcggctaaga atctggataa gccgtttcac
     6541 ttctctcgaa cgaccaacaa ctgggtccag cttgttttct tttgctaatt tcgtcaaatc
     6601 acgtgctaat gaatctagcg taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt
     6661 tacatttcta cgacgattca ttccttttcc agcagaatca ccagctagcc ccattttttg
     6721 tttcagaact tgacgggctt ttgaaaggct gatacctaag ttgatcatga ttcgagtagc
     6781 catgatactt tcatctttta ataagctcat caaaatatgt tctgttccaa ctttttgaga
     6841 accagttttc ttcacttcgc tgctagctaa agcaaaaact tgtttcattc ttggtgaata
     6901 tggaagaaag actcctttag ggtaagaacg gataccacca taaccaatca gatgttcaat
     6961 ttcttcataa atttccgttt cattcaaatc catttcacgt aatacttttc cagcaattcc
     7021 attttgctcg accactaagg ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa
     7081 ccgttttgct tcttcttgtg caatttctaa tactgcttgg gctctttcag taaaaagttc
     7141 ttccatctaa tctcacttcc catcttcata acttaaacgt tccagaaatg ctcggagaat
     7201 ccgagcacgt gtatgatctt catattcatt gaaattcaat gtgttttttg caattgcact
     7261 caacatcaag tttccttctt tttttgtaat tatttggtct tcataaagtt tttgaataat
     7321 agcaaaagca tttttctcac tgattgtatc atcaaataac tgatttatct gctcaagtaa
     7381 ttgtttttta tctgaaatcc ttacttttgc aattcgtata taaccaccac caccacgttt
     7441 gctttctact agataaccac gttggatggt aaaacgggta ttaatcacat agttgatttg
     7501 tgaaggaaca cagttaaaga ggtctgccat ttcaatcctt cgaatctcga tcatttcact
     7561 attttccaga attttcttta tatatgattc aattaaatcc gaggtattct gatgtgccat
     7621 cttattcatt ccctttgact aatattgacc ttaattataa cgaatttgat aatagaaaga
     7681 aagaagaacg ctttttgtta aataaatcct ttagaatcaa caaaaaaaaa tacggaatat
     7741 actattccgt atcgacgcgc ccaagaggag tcgaacccct aaccttttga tccgtagtca
     7801 aacactctat ccagttgagc tatgggcgca tattcaattt aaaaaaatta taaatcattt
     7861 gaaaacaaaa agcaagataa aaaaaatcgg gaagacagga ttcgaacctg cgaccccttg
     7921 gtcccaaacc aagtgctcta ccaagctgag ctacttcccg tttaaaaaat aaaaaagac
//
LOCUS       DAFCNJ010000086         7926 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000086 DAFCNJ010000000
VERSION     DAFCNJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.091"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..199)
                     /locus_tag="LNE35_002587"
     CDS             complement(<1..199)
                     /locus_tag="LNE35_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911685.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="LNE35_002588"
     CDS             complement(362..1996)
                     /locus_tag="LNE35_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6911686.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="LNE35_002589"
     CDS             complement(2000..3373)
                     /locus_tag="LNE35_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6911687.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="LNE35_002590"
     CDS             complement(3389..3628)
                     /locus_tag="LNE35_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911688.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="LNE35_002591"
     CDS             complement(3810..6485)
                     /locus_tag="LNE35_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HBK6911689.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="LNE35_002592"
     CDS             complement(6668..7591)
                     /locus_tag="LNE35_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911690.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2328 a         1560 c         1198 g         2840 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ttagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactaaat tctataacat tagaagaaat tttaaatacc tttgataaaa
     7921 aatatt
//
LOCUS       DAFCNJ010000087         7760 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000087 DAFCNJ010000000
VERSION     DAFCNJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.092"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(288..2474)
                     /locus_tag="LNE35_002593"
     CDS             complement(288..2474)
                     /locus_tag="LNE35_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK6911691.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2692..3375
                     /locus_tag="LNE35_002594"
     CDS             2692..3375
                     /locus_tag="LNE35_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBK6911692.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3456..4175)
                     /locus_tag="LNE35_002595"
     CDS             complement(3456..4175)
                     /locus_tag="LNE35_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK6911693.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4487..4693)
                     /locus_tag="LNE35_002596"
     CDS             complement(4487..4693)
                     /locus_tag="LNE35_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911694.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4938..5540
                     /locus_tag="LNE35_002597"
     CDS             4938..5540
                     /locus_tag="LNE35_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK6911695.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5681..6961
                     /locus_tag="LNE35_002598"
     CDS             5681..6961
                     /locus_tag="LNE35_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6911696.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6974..7702
                     /locus_tag="LNE35_002599"
     CDS             6974..7702
                     /locus_tag="LNE35_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6911697.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2229 a         1449 c         1299 g         2783 t
ORIGIN      
        1 tattagctaa taatatataa aaaaaaatca aaataactga ttatttacgt ttgaataatg
       61 agaatttttc tatatattct ctattttgat aatagtattc tcattttcaa aataaataag
      121 ggaggtgtga caaaattctt gtcacacctc ccttttccga ataaactgtg ttccagaaat
      181 agcttcttcg aaaataagct gaaatttcac aaaaatttga gaagcaattt tcgtacattc
      241 cttcttcttt cttgaaactg accacttctg tcacaccctc tcttctatca ctttcctttt
      301 ttcacacgac tcccggtttc tttgataaat ctcgatactt tcaattttct tgaatttttc
      361 atttgaggta cacatagata cagacgattt cttgctctag tcatcgcaac ataaaatagt
      421 cttcgttctt cttctatcgt cgtcactggt tctgctttta tttcattaaa gtcgattact
      481 ttcttttctg tcgctccgtt ataaggcaaa cttccttcgt tacaaccaat caaaaagaca
      541 tttcggtaac taagaccttt acttttgtga atgctctgga tacatagtgc atcaagtttt
      601 ccttgtctgt aagcaatcaa gcgggttttt aattctttag ctgtcgcttg ggtttcttgc
      661 gataaggctt ggagtcttct gaaagtagca tgctgcttta cttcttctcg tatccattct
      721 tgatcactct ggtcgatttc cttagctata cgtggcagat gaagatagct ttgaacaagc
      781 gaatagattt cttgatgctg gtctgtttta cgcaacgctt cctcaaactc tcggatttct
      841 gcagatttcg attttaaatt ggggcttatc gacaaaatat aatctgcaac ggctatattt
      901 tgatatttag gagaaaagta ttgatcgcat tcactttttt tcaggtagca gtctgccatc
      961 agctgccgga gcaattgctg gtaggcctga tttttctttt gcagattcag tgctgtttgc
     1021 caatgaacaa ttttttgata gaaatgttgg aaagacttga taccaaagat accttcatat
     1081 ggactatctg cttccagtac aaaaggaata ttcgttttca gcagagattg gacaagctgc
     1141 tgcgtttgtg aagctgatcg gtataatatg attgattcct tatatttccc atttttttga
     1201 ataaatgtct gaatttgttt gattagccac tgactttctg ctttagtatc agaaaacaaa
     1261 taggcttcca taacacctgc ttctttttgt gctggaatca tcgcctttgg tatccgatca
     1321 tttttattat agctgatcaa tctatttgct ccttggacaa tagctgcccg agaacgataa
     1381 ttagtcaata agaaaagagt tttggtagga tattcttttt caaagttctt gatgtatgct
     1441 ggctctgatc ctctgaatcc atagatactc tgatcatcat ctccaacaac aatcaacttg
     1501 atcctgtttt cctttgtcat ccgtttcacg atttcccatt gaagtggatt agtatcttga
     1561 aattcatcaa tgaatagata atcatatttt tggccgatat aatgctggat ctcctcattc
     1621 tccaaagcgc ttttcagatt ttggagtaca tcattgaact ctagcaattg ctggcttctt
     1681 ttttgttctt ggagttccgt gagcaatcca tacagtcgtt ctgatatcgg ctgtcgtgca
     1741 ttcttatcaa tggaatcata tttttcttta actaactgaa ccatttcttc tactgaaagg
     1801 ttcatattct gccaatagtc taaccgagaa gcaatcgttt tttctccata ttgatcaaat
     1861 atataaagat tatatttacg tatcagctgt tgcaaaacag cattcacttt ttgtgcagta
     1921 tcctttgaaa agtctccttg tattccttgc attgctactt caggaaattt tcgacaaatt
     1981 tcattgtata tcctaaaaaa gatactatgg aatgttccga tatggacagc ggtttttctc
     2041 tggctgaaat tatctactcg tttttttagt tcatttgcag cgttccttgt aaaagtgatc
     2101 attagtatgc gttcttcgtc tacttgtttt tgcgtcatca gataaacagc ttttgcacag
     2161 attgtttgcg ttttgccgct cccagctcct gctatgattc gaagatgttt cttcatatca
     2221 tatgtgatag ctgctttttg ttcttgattg aagcgattat aggaacgttt ctccatcatt
     2281 ttaaagaaag agctgtttcc ttcactttct tttacttgta cttcttcctc aataagtccg
     2341 ggtgcttcgc cataagccgc ttcataggta gcaatgattt cttcttgtag ccgaaagact
     2401 tgttgaagct ttttatactt gttctcccag cgtttcaatt ctttttgtaa tcggagaatt
     2461 tctttttcat tcaactgatt gatttccttc ccatctttct tctttgaatt tgtttatttc
     2521 aagttggtta tttaaagttt cgttgtctat tttttcttta tactaacaga cctttctaaa
     2581 aatgaaaatg ggaagcgata tgattttagt tttctgaaaa tttttccttt ttttcaatcg
     2641 tttatgctaa gataaggaag acaaaaatat tcataaaagg tgatgaaaaa aatgcagact
     2701 tcctttcacg aggggcgtac aataggactt ttgctgacat ttatcggagg agccatggac
     2761 agttatactt atatccaata tggtgctttt gcctctgctc aaactggcaa tatcattcta
     2821 tcgatcatcc aagcatttga cggagaatgg ctaagtgttg gtaaaaaagt attgtctacc
     2881 cttttctttt tcttagggat tcttctaaca aagtatctaa ttgattattt tagaaaaaaa
     2941 gaaaaacatt tctggcgctt gtttgttcta tattatgaag cagtgatttt tttctttgtt
     3001 agtttgacac ccgtccatga gcatcccgca ttagtaacga ttttgattgc ttttactgca
     3061 tcgatccagt gggtagcttt tgataaaatc aacgggcgtg cctacactaa tttatttaca
     3121 actggaaatt taaaaggggt agcaacgaac ttatatgatt attgtatttc taaagaaaaa
     3181 gcagattttg atcgattttt ccactatcta agtgtcgttc ttgcctttat cgcaggagct
     3241 attgtccttg ttttctgtca tcatctgttt ggcgcccatt cgattttgat cgtttccatt
     3301 ttatttttga tcatggccct cactcagaca tttcatttat ggcaatttta tcatacgaat
     3361 cggttcacta tttgatctca atgaatcaaa aaaactaagc aagatgctgc tcacagagaa
     3421 atatctgcga gtgagcaatt tgcttagttt ttatattatt tatttgtaaa gattcccttt
     3481 gctttcgggt tatttgtagt gtagcggatc gactgggcat ctgcctcttc tgtcattttc
     3541 tttgcccgca tcttgatttc ttgtacgatt tgttctccac atgccggatc ggctacgaaa
     3601 tcatatttgt ccccatcgat cactattttg ggacatatat caaactgttc ataccactct
     3661 tcgtatcgtc gattgagttc tttatagtag gaatagagtt caggatcttg ttctagctgt
     3721 tcatattccc ggcctctttt tttgattctc gctaacatag tttcaaagga tacacgaata
     3781 taaaccagta gatctggttt cttttgtggc gaaatttctt ctaactcctt cagcatcgtt
     3841 tctaaaagac cttcatactg ctttacttct acttcagaaa cacgccctaa atctgcattc
     3901 aaatgaaaca gcatactatc ttcgtagatg gagcggtcta gtacgttgtc atcatggctg
     3961 aaagcatctt tgattgccaa aaagcgtttg ttcaaaaaga aaatctgtaa taaaaaagta
     4021 tacttttctg gattagaata aaatagcggc aatacttcat tatcttccac gttctcataa
     4081 aaaggttttg tttctaaatc ttgggcaagc atttcagtta gtgtgctttt ccctgcaccg
     4141 atcgttcctg caacaatgat cacaattatc ggcatccttc ctgtaataaa atctgttctt
     4201 cattttacta cttcatttat agaaataaaa gccttgactt tatctgctcc ttaaaagcta
     4261 cttctctaaa agtagccaaa agtaactcct attcatgaaa ataaaggctg tgacaaaagt
     4321 cttgtcgcag cccttttttc aaataaacag tattctcctg ccaacacctc ggtcacaagg
     4381 caatattcat aaaacatgaa aagctgtttt ttaacattat tccttgtaac tcagagctaa
     4441 acgggagact cacaacctct ttatgctctc aacattttat ctgtttttat tcttctattg
     4501 tacttgattt gcgtacaact aactgccagt gcagattatg agcggtatca ttgtcttcta
     4561 ctgcgcattt catgacacgt atcatcacac tattttcata gattttttct atttttcctt
     4621 ttattggtga ttcaaataaa tcagatttaa atgcatatgt ttcgccgact tcaaacaatt
     4681 gttcattttc cataagaatc cctccatcac cttttataag gtcagtaatt ttttaacata
     4741 aatcactcga aaaagcacaa acttgtcata aaaagtactt ttcgtatcaa aactgtcact
     4801 ttttttggta aggttcttgg aaaacgaaga tttttttagg agatcatgac ggttttgatg
     4861 tcaaaaccac tttttatgaa acaaaagacg ttttttattt tttttagcat aaaatttgtg
     4921 gtggaggtac gtatgagatg aaagataaag ttgtgaacaa attattagaa gccttattca
     4981 gcggagacaa atcggaagaa gatatggtgt atgtccaagt aaaatttgcc ttagaagttt
     5041 tattgaataa tcttggaaaa ctagcggtcg ttctagtctt ttctctggtg acaggctcat
     5101 gggcggaaac aggaatcacc tttctttctt atatctgcat ccgcagatat gcttatggat
     5161 tgcattccga ctctgaattc gtttgtctgc tgtggacact actttattta tggggcgttc
     5221 ccttggtgat gaaacatctg caattgacta tctccttccc aatgatggtc tttttgttaa
     5281 tcagctgctt tttgttactg cttcgctatg gatcaagagg tacagcaatc aatcccattg
     5341 aacccgagaa acgtccccca ttattaaaga aggcgatcag catgtttcta attttttctc
     5401 tcattacttt atttttctca gcttcttact tttctaccta tttgctactt gggattgttt
     5461 tagaaatagc tacgttgctt cctattacaa actacttgat gaattttgga ggaatctttc
     5521 atgaaaaaaa caatcattaa acaattaact aacttatctg acttttttgt atctggctgc
     5581 tgctttggta tgagttctga attcaaaatc cctgaaaaat taaaaaaata atacgctaat
     5641 tgtgctataa tttggtaaaa atcaatggga gctgtacttt atgtttttat ggtccatagc
     5701 gttgcaatat ttgatttaca caatttgtat cttattgatt gaccatcaca tccgatacaa
     5761 atatctgttt gcgggagtca ttctgatttc ttgtattttg attggatggg tctattcgat
     5821 tttagggatt tttactgctc taattgctat aggaatgctg atgcttactt ttttcttctt
     5881 ttcgacttcg cgtcaacaat tggtttacgc gcttcctttt gggttattga cgtcgctttt
     5941 aggagatcac ttaacaagca tcgacgattt ttttgttttg aaatcttcgg ttattgaacc
     6001 aggagattca ttgcagtatt ttcacattgt cttatccgct tttttatctg ctttattagc
     6061 tttttgttta aaaaaatcgt tagagacttg gtttcacact gtggaccgcc gtatgatcgg
     6121 tacgatcggg acattgatat tatttacata ctatatcatt attttttata cccgtttttc
     6181 tggtgaaaca cctgatgtct tagctttgaa tacgagtttt ttcattctat atctgtgtgt
     6241 tggcctgatc atcttggttt attattggaa aatctctaat aagaaattag ctgatcagtt
     6301 attagagcaa caagtcagga tgcaacagga ttatatcaaa gatctagaaa aaaactatca
     6361 agacttgaga gaattcaaac atgattacca aaatctgctt ttttctcttc acagctatat
     6421 ttcagaaggc gacttagaag gattacggtc ttattatgat caaacgatat tacctacacg
     6481 ccagatgatg aacctttttc cggccaatct aagtatgttg gataatatga aagttcctga
     6541 gatccgtagt ttactgtcat taaaactcat gatggcacag gaaaaagggt tgatcgtcca
     6601 gcttcatttt cctgagccaa tcgaactgga tacgaaacat acggtaaacc ttgtacgtat
     6661 gcttggtatt atcttggaca atgcgattga aggagctagt gctgcaaaga agaaaaaaat
     6721 cgaattgacc atactcaaga agaatcattc cgtgatgatt cgtgtgatca atacaacaac
     6781 aaatacatta ccgttaaacc agttgaaaca aaaagggttc tcaacaaaaa caaatccaaa
     6841 aaacgaaggc cttggcttgt ttatcttgga tgaattgaca aagaccaatc cttatctttt
     6901 ccttgaaaca tcaattgaaa accagcgttt ttctcaaacg atttatatac aaataaaata
     6961 aaggagtaat gatatgattc ccatttatat ttgtgaagac gatccaaaac aattagaagt
     7021 tttatcaacg gtgatcaaaa atagaatcat gattgaaaac ttagacagtt atgtccatat
     7081 ttctacctct gatccggaag aacttctcaa tgcagccaag atcagaactt cttcttttgg
     7141 tatttacttt ttagatatcg aactagaaga cagtgaaatg gatggtgtag cgatcggtaa
     7201 attgatccgt gaattagatc ctttaggaaa aataatctat gtgacttccc atacggaaat
     7261 gtcgatgaaa atcttaaaaa gcaacatcga accgactgat tatatcgtca aagaagatct
     7321 ttatgattta aaagaaaatg tcgagcatat cctgagcaaa attttcgaag attttcaaat
     7381 gtcccaaatc caaaaagata tcttcaaaat agagtttaat gatgagatca aatttttacc
     7441 gatcaaagac atccaatatt tttctacttc gctaggagcg ccacataaac tggaagtcca
     7501 tttgactcat gcccaattac aattctatga gaagatcaaa gaaatcgaga aaatgcatcg
     7561 gaattttatc cgttgtcata aatcctatgt tatcaataca caaaatgtgc ggtcgattaa
     7621 taaaaaaacc agagaagtca cgttggcaaa cggcgagacc atcccagcct ctatccgtgg
     7681 attaaagaaa ttgatttcat aaaaagaacc gaatagtttg gaccgactaa atgaccagcc
     7741 gatccaaacc cttcggttct
//
LOCUS       DAFCNJ010000088         7697 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000088 DAFCNJ010000000
VERSION     DAFCNJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.093"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..179)
                     /locus_tag="LNE35_002600"
     CDS             complement(<1..179)
                     /locus_tag="LNE35_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBK6911698.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="LNE35_002601"
     CDS             complement(192..1295)
                     /locus_tag="LNE35_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBK6911699.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2341)
                     /locus_tag="LNE35_002602"
     CDS             complement(1385..2341)
                     /locus_tag="LNE35_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBK6911700.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="LNE35_002603"
     CDS             2622..4556
                     /locus_tag="LNE35_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6911701.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="LNE35_002604"
     CDS             5013..5429
                     /locus_tag="LNE35_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBK6911702.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="LNE35_002605"
     CDS             5443..6009
                     /locus_tag="LNE35_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBK6911703.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="LNE35_002606"
     CDS             6032..7018
                     /locus_tag="LNE35_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK6911704.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7697)
                     /gene="scm"
                     /locus_tag="LNE35_002607"
     CDS             complement(7173..>7697)
                     /gene="scm"
                     /locus_tag="LNE35_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBK6911705.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2203 a         1473 c         1591 g         2430 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgcta tcacttgtgg tagaggtagt atctgtactg
     7681 tcacttgaag ctgatgt
//
LOCUS       DAFCNJ010000089         7600 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000089 DAFCNJ010000000
VERSION     DAFCNJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.094"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(943..1356)
                     /locus_tag="LNE35_002608"
     CDS             complement(943..1356)
                     /locus_tag="LNE35_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBK6911706.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="LNE35_002609"
     CDS             complement(1812..2297)
                     /locus_tag="LNE35_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911707.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="LNE35_002610"
     CDS             complement(2436..2651)
                     /locus_tag="LNE35_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBK6911708.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="LNE35_002611"
     CDS             2875..3675
                     /locus_tag="LNE35_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6911709.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="LNE35_002612"
     CDS             3694..5562
                     /locus_tag="LNE35_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK6911710.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="LNE35_002613"
     CDS             5555..6046
                     /locus_tag="LNE35_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBK6911711.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="LNE35_002614"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="LNE35_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBK6911712.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="LNE35_002615"
     CDS             6606..>7600
                     /locus_tag="LNE35_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBK6911713.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAFCNJ010000090         7115 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000090 DAFCNJ010000000
VERSION     DAFCNJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.095"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            94..1248
                     /locus_tag="LNE35_002616"
     CDS             94..1248
                     /locus_tag="LNE35_002616"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBK6911714.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="LNE35_002617"
     CDS             complement(1270..2022)
                     /locus_tag="LNE35_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6911715.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="LNE35_002618"
     CDS             complement(2386..5052)
                     /locus_tag="LNE35_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6911716.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="LNE35_002619"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="LNE35_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK6911717.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2099 a         1467 c         1290 g         2259 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtt
//
LOCUS       DAFCNJ010000091         6706 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000091 DAFCNJ010000000
VERSION     DAFCNJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.096"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(7..1164)
                     /locus_tag="LNE35_002620"
     CDS             complement(7..1164)
                     /locus_tag="LNE35_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6911718.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="LNE35_002621"
     CDS             complement(1167..1523)
                     /locus_tag="LNE35_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911719.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="LNE35_002622"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="LNE35_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBK6911720.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="LNE35_002623"
     CDS             complement(2991..3830)
                     /locus_tag="LNE35_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6911721.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="LNE35_002624"
     CDS             4015..4902
                     /locus_tag="LNE35_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK6911722.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="LNE35_002625"
     CDS             complement(5486..6181)
                     /locus_tag="LNE35_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBK6911723.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="LNE35_002626"
     CDS             complement(6165..6563)
                     /locus_tag="LNE35_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK6911724.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2108 a         1319 c          987 g         2292 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catctctgtt cctcctcgta aaatatcatt tgtctaatag
     6601 tttttattca aaatgtttct agattataaa atttattgaa taacacattc ctaacggaat
     6661 ttgtttcgat attgtgtgat ttctcagagt acaatggatg tatgga
//
LOCUS       DAFCNJ010000092         6643 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000092 DAFCNJ010000000
VERSION     DAFCNJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.097"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..859)
                     /locus_tag="LNE35_002627"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="LNE35_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="LNE35_002628"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="LNE35_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBK6911725.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="LNE35_002629"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="LNE35_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="LNE35_002630"
     CDS             complement(2143..2373)
                     /locus_tag="LNE35_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBK6911726.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="LNE35_002631"
     CDS             complement(2603..3421)
                     /locus_tag="LNE35_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6911727.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="LNE35_002632"
     CDS             3582..4271
                     /locus_tag="LNE35_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK6911728.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="LNE35_002633"
     CDS             4285..5787
                     /locus_tag="LNE35_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6911729.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="LNE35_002634"
     CDS             5800..6276
                     /locus_tag="LNE35_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6911730.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="LNE35_002635"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="LNE35_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAFCNJ010000093         6600 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000093 DAFCNJ010000000
VERSION     DAFCNJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.098"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..486
                     /locus_tag="LNE35_002636"
     CDS             <1..486
                     /locus_tag="LNE35_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911731.1"
                     /translation="LIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLKDESNTTVSD
                     ANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKTGADIDHFDK
                     ALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQETIKKLRIG
                     G"
     gene            493..1344
                     /locus_tag="LNE35_002637"
     CDS             493..1344
                     /locus_tag="LNE35_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBK6911732.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSEPKNASGAYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            1356..1625
                     /locus_tag="LNE35_002638"
     CDS             1356..1625
                     /locus_tag="LNE35_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911733.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            1630..1794
                     /locus_tag="LNE35_002639"
     CDS             1630..1794
                     /locus_tag="LNE35_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911734.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            1787..2089
                     /locus_tag="LNE35_002640"
     CDS             1787..2089
                     /locus_tag="LNE35_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911735.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            2086..2397
                     /locus_tag="LNE35_002641"
     CDS             2086..2397
                     /locus_tag="LNE35_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBK6911736.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            2394..2612
                     /locus_tag="LNE35_002642"
     CDS             2394..2612
                     /locus_tag="LNE35_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HBK6911737.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            2623..3438
                     /locus_tag="LNE35_002643"
     CDS             2623..3438
                     /locus_tag="LNE35_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBK6911738.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            3577..3933
                     /locus_tag="LNE35_002644"
     CDS             3577..3933
                     /locus_tag="LNE35_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBK6911739.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            3920..4138
                     /locus_tag="LNE35_002645"
     CDS             3920..4138
                     /locus_tag="LNE35_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911740.1"
                     /translation="MDLYDIESGATVSVESDHLIISFADERQIIIWVVDDMLYPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            4135..4533
                     /locus_tag="LNE35_002646"
     CDS             4135..4533
                     /locus_tag="LNE35_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBK6911741.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4530..4868
                     /locus_tag="LNE35_002647"
     CDS             4530..4868
                     /locus_tag="LNE35_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911742.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4865..5095
                     /locus_tag="LNE35_002648"
     CDS             4865..5095
                     /locus_tag="LNE35_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911743.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            5102..5305
                     /locus_tag="LNE35_002649"
     CDS             5102..5305
                     /locus_tag="LNE35_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBK6911744.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            5302..5607
                     /locus_tag="LNE35_002650"
     CDS             5302..5607
                     /locus_tag="LNE35_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911745.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVINNGIKN"
     gene            5591..5884
                     /locus_tag="LNE35_002651"
     CDS             5591..5884
                     /locus_tag="LNE35_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911746.1"
                     /translation="MELKIDKVTFEEPAFQEMDERIAELVEKKQDELIALLEKKYQFP
                     KYMNKMQAAKYLGVSYNTMMDKYVPAGLKMGIVNGVIRISKEECDRFMKEHQK"
     gene            6144..6560
                     /locus_tag="LNE35_002652"
     CDS             6144..6560
                     /locus_tag="LNE35_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK6911747.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2470 a          969 c         1497 g         1664 t
ORIGIN      
        1 ctcataggcg atattttcat cgatacaggc atgccaacgg acttctggaa ggaattcttt
       61 tacttccgct ttgaaggtgt cacagggcgc aaaataagcc tcaaagatga atcgaatacg
      121 actgtgagtg atgctaacgt cttagcaaat atcatcttag atttcatctt tgaacatcat
      181 attcctttca aagaaggcta tgagatttta ccagcgaatc aagaatatta cttctacaaa
      241 tgcatcacaa aaagagtctg ttgcatctgt ggcaaaacag gagctgatat cgatcacttt
      301 gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc attcagagta cacatttgca
      361 gcactctgca gaatccatca cacggagaaa cacaagatag gtgtgatcaa tttcaaaaat
      421 aaatatcaaa tcaaagggat caagttaaac caggaaacaa ttaagaaact tagaatagga
      481 ggataaaaaa tattggctga caacaaacga tactactatt taaaactaaa agagaacttt
      541 ttcgatagcg acgagatggt tcttttagaa agtatgccag atggatacat ttattctaat
      601 attcttctca aactttattt aagaagtctg aaacatgaag gcaaactaat gtttaatgac
      661 aggattccat tcaattctac aatgcttgcg actattacaa gacactctgt gggagttgta
      721 gaaaaagcag tacaaatatt tcgtgattta caacttattg aggtattaga taacggagca
      781 atttatatgt ctgatataca aagttttatt gggaaatctt caactgaagc tgataggaag
      841 agaaaataca gaaaagaaat tgaggaagca aaacagaatt taataacaag tggacaaatg
      901 tcggacaaat gtccggacaa aactacacca gagttagaga tagagttaga gaaagatata
      961 gagttagaga aagatataga gaatgtaacg ccttcgaaaa aatcgaaggc taagcccgtc
     1021 cgtcataaat acggagagta taaaaatgtt cttttgtcag atgagcagat ggagaaactc
     1081 aaaatagaat tccctaatga ctatcaagaa cgaatagaac ggctatctga gtattgtgaa
     1141 tcatctggta agacttataa aaactatttg gcaactattc gaagttgggc aaggaaagaa
     1201 aaaagtgagc ctaagaatgc aagtggtgca tacaagcgca caggaagacg agagaagctt
     1261 cctgaatggg caatcgacca agaagcctat ctcaagaaaa aagcgctaga acgagctaat
     1321 agacaatcaa aagcaccatt ttaagaggtg gaaaattgaa aatcgattat ctagaactaa
     1381 ttaatgaaat agcaaagtat aaaactggtg aggaaataga aatcctgaga gacgtatatg
     1441 atcaactcga agaagctgga atcgaaggaa ttaataatga tcgttcgagt tggagtaaac
     1501 tcagatacta tttcgcactc tatatcgata caacacaatt aagaaattta gcttatacaa
     1561 aattactatt tgttgattgc gttaaaggat tgcaaaaaca tcttagtgaa cttgagcagg
     1621 tgtaatcaga tggatctaaa aacatttaca gcacagatcg aactaatgca tcaagaagct
     1681 ttaagacaaa gcgcctcgta cgaagacaag tggctcaaca cgttccatgg tggacgtgag
     1741 agcgcacttg atcaagtact caaattattg aaaggggaac gtcgggatgg ataagaaagc
     1801 ggcaatgaaa cgaatcatcg aactgacaca ttctgagaat tggcaagaag acaaagaaat
     1861 agttacagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac
     1921 gccgagaaag attgcaattt ggcatggtga tcgaattcta gtaacaggta ctgctgaaca
     1981 gttatctgaa attactggtc tgagcaaaaa catcatctgg gatagagcaa ggagcttatg
     2041 gattgattca aaaggacgac aatttaggta tgtggaggag aaaaaatgaa cgaactaatc
     2101 acaaaagtag agcagtgggc taaagacaag ggactggatc aagcagatcc aaaagcacag
     2161 tttttgaaag tagctgagga attcggggaa atcgcttcag caatggcaag aagtaatgat
     2221 gagctattta aagatagcgt aggagacgta atcgtcacgt tgattatcct ttccatgcaa
     2281 aaagggacaa acgtacaaga gtgtttagaa atggcataca acgaaatcaa agggcgcaca
     2341 ggaaaaatgg tagatggtgt attcgtgaag tcgagtgatt tggaggacag caaatgatag
     2401 gtttaacaat attttggtgt gcgattggtg gtttactagc atttctagga tactacctca
     2461 tctgtgaaag tagaaaagga agaaaatata cagtgacagg tattgtcctg ttaattatcg
     2521 gaattctagt tgcagttgga gcagaactga atctttatgt aaatggtaca caagaagatt
     2581 tggtgcgatt tatgttttgg atgaagaatt aggaggaaaa aaatgaacga aaacaaatta
     2641 atcaaattag gtgtagcagg agcagtaatc gtaggtattg gagttatcgg aggatttaag
     2701 ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa cggcggtatc
     2761 aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt gatccaatat
     2821 ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag cgacggcaaa
     2881 aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc agcaaaaatg
     2941 tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt aaaatctaag
     3001 cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt cctttcagga
     3061 gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt tgaatctaaa
     3121 gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga aacacaaaaa
     3181 tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct agatgcagaa
     3241 actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc agaagcagag
     3301 gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg ttatgaagaa
     3361 gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac tgtagttacg
     3421 gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt tcttaggttt
     3481 agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca aagaagatga
     3541 caacaacgat gaaatgaaat tttaggagga gaaataatgg atctcattac acaatacagt
     3601 gatatcatcc tcaagaaaat catggcaaag attcagaaag ataaaaaagc aaaagaacga
     3661 gcggaattag ttaagttgga aatggctgaa acaggagtag gagtgcgaag tagcaggcat
     3721 tggaaagcag cagcaaacat tgaattttat tacaacgaaa ttcaaaaagg gttcgatcag
     3781 atgcgtgagc tggatcggca aacaaattgg agcaagaaac ttcatcaaga tcgtttcaaa
     3841 tttgtagaga agtataaaga aatattagaa gagtatttga ggaggacagc gaatgataaa
     3901 aaagctcatt caattcagca tggatttata tgatatcgaa tcaggagcaa cagtatctgt
     3961 ggagtctgat catttaatca taagttttgc tgacgaacgt caaattatca tatgggtagt
     4021 tgacgatatg ctatatccag aaattgtaca tgattttgaa gaatcaaaag cggttgagtt
     4081 tgaaatagtg aaaaaagtaa tggaattgat tgaaaaatac gaggaggaca gcaaatgata
     4141 ccgaagttta gagcgtggga taagcgagaa aacacaatga gagatgtagc tgtcttacat
     4201 tttactaaaa acggcaaaac aaactttatt gaatattgga taaatcctac cgaattgaaa
     4261 tcatatcacg tgcgaaacat cgacctcatg caatccacag gaatgaaaga taagaatggt
     4321 gtggagatat ttgaagggga tgtagtaatt acatcaaaag gagcgattgg atatgttgcg
     4381 tatcttcaac aagaagccgg ctttgtagtt gtactaaaga aaagtgatta tagattaggc
     4441 cataggaata ctggcgaaag ttatgatgta gcaactgctc atgaagtcat cggaaatata
     4501 tacgagaata gcgagttatt ggaggtagag tgattatctt agctactgac tacatcgatt
     4561 ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa ttgtggaatt aaaagcaaga
     4621 atagaatcat tttgttacgg atatcggctg atttagcaaa atatctttat ctgagcaaga
     4681 cagcagtata ttacactgcg gctacacgtt atcagtacaa gggaaaaaca atcagtcagg
     4741 attattatga tcgtttaatc agtcctgaca tgcatggaaa atcaaaatca gcgatcaagt
     4801 tatttggcgc aatagagata tatgccgatg attttccaaa tttatggttg aaggaggaag
     4861 cggaatgaaa tacgaaatac cactaagtga agcgggcatt caagcaatta tcaatggtcg
     4921 ggaggttaat aaaaaacttc ctgatggtac tgaattagtc atcagacaaa gttatttgaa
     4981 agatatggca gctccagtat taattgatcg ttttaacgtg actgattctg tggtagagaa
     5041 ccacttgaaa gaatttcgat caagtataaa cgacactttc agattaggga gttgattgac
     5101 aatgaacacc agacaccgca gagtagcaaa actaagaaaa caggaactga atgtactaaa
     5161 gacaaagttt gaaaaagaat atggaatttc agcagaagaa acatataaag tggcaagtca
     5221 gtttgttgct gatgcgagtg agactattcg taagtttggg atttcgatat taaatgatga
     5281 tcgtaaatgg gaggcagaaa gatgaaacta aaagacggat tttacactga tgcttatgga
     5341 attggtggtt tgatgatgga tctaccaact aagaatccta taaagcaaga ggaatcagaa
     5401 attaaagtcg gtgacatggt tcgctgcgaa gcagaagggt tcatctatcc atttcgtgga
     5461 tatgtagagc atctctataa tcactcagcg atcattcgta ttgaaaacac aatggaatgc
     5521 gataagtggt tagcgaaaag caaagagaat ttagcagtgg tgagattggt ggatatggag
     5581 gttataaaca atggaattaa aaattgacaa ggtaacgttt gaagaaccag catttcaaga
     5641 aatggatgaa cgaattgcag aattggtcga gaaaaaacaa gatgaattga ttgctttgct
     5701 agaaaagaaa taccaatttc cgaaatatat gaacaaaatg caagcagcaa aatatcttgg
     5761 tgtaagttac aacacgatga tggataagta cgtaccagct ggtttgaaaa tgggtatcgt
     5821 taacggcgtt attcgtatca gcaaagaaga atgtgatcgt tttatgaaag aacatcaaaa
     5881 ataaaataaa aaaacgttta gccccttagc taaacgcaca attataattt acctgacagt
     5941 taattataac aaaattgcgg aggttgagga atggcggaat gaaaaagaca ataaaaacag
     6001 aagaagcata tattgaagta tttgacacac cagcatgcta agtcgagaag tgtaactcgg
     6061 caaataaaaa aagccggatc gctccgactg atgtaataaa cccaacaagt ttattatatc
     6121 acataaagga gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa
     6181 gatgcaatgc gagagatgtg ctgaagaatt ttcgacgctt agagcggatg gcaggtcgct
     6241 ctttgataga tattaagtcg ccgattatta cggatatgcc gaaggcaccg aagcacggca
     6301 ataaggcaga ggacgcgatt attcagatga tggatataga agcagagaga gatgcgattc
     6361 tagcggcttt gatggctctt agtctgatta gccgtcagat actctattat agcttttgtg
     6421 acgtaaacaa gcactctaat tatgaaatag ggcaattgat acgaggatac ggagagaaga
     6481 atgtagagaa gctgaaatct atcgcgctga tcgaatttgc tgaagcatac aaaaaaggcg
     6541 tattagttca gtatcgttga ttttgtaggg tttttgtagg gatagtgtag ggtttttgag
//
LOCUS       DAFCNJ010000094         6570 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000094 DAFCNJ010000000
VERSION     DAFCNJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.099"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..579
                     /locus_tag="LNE35_002653"
     CDS             127..579
                     /locus_tag="LNE35_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911748.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="LNE35_002654"
     CDS             593..1282
                     /locus_tag="LNE35_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911749.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="LNE35_002655"
     CDS             1407..3758
                     /locus_tag="LNE35_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBK6911750.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="LNE35_002656"
     CDS             3838..4233
                     /locus_tag="LNE35_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911751.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="LNE35_002657"
     CDS             4558..5175
                     /locus_tag="LNE35_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6911752.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="LNE35_002658"
     CDS             5216..5515
                     /locus_tag="LNE35_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBK6911753.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="LNE35_002659"
     CDS             5549..5716
                     /locus_tag="LNE35_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6911754.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="LNE35_002660"
     CDS             complement(5761..6366)
                     /locus_tag="LNE35_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6911755.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DAFCNJ010000095         6456 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000095 DAFCNJ010000000
VERSION     DAFCNJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.100"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            414..620
                     /locus_tag="LNE35_002661"
     CDS             414..620
                     /locus_tag="LNE35_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBK6911756.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(822..1823)
                     /locus_tag="LNE35_002662"
     CDS             complement(822..1823)
                     /locus_tag="LNE35_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6911757.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1828..3741)
                     /locus_tag="LNE35_002663"
     CDS             complement(1828..3741)
                     /locus_tag="LNE35_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBK6911758.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3909..4415)
                     /locus_tag="LNE35_002664"
     CDS             complement(3909..4415)
                     /locus_tag="LNE35_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6911759.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4575..5015
                     /locus_tag="LNE35_002665"
     CDS             4575..5015
                     /locus_tag="LNE35_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK6911760.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5174..>6456
                     /locus_tag="LNE35_002666"
     CDS             5174..>6456
                     /locus_tag="LNE35_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6911761.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT         1832 a         1314 c         1047 g         2263 t
ORIGIN      
        1 ttatttttgt ttcttatttt ctaaaaaatt aaaaaaaatt ttggtgaatt aagtatttcc
       61 ttgataacac tggcttttgg cctcatttta ggttatcatt tgtaatttta tttcacagtt
      121 gatatgattg ttatgtaaaa taaaaaagtt taggatacat cgaatttttg gggaaattct
      181 tttgggggta tcctaaatct tttctattat attcccatat tttctattat tggtctagac
      241 gtatgatggt aaactttgta cctatctgat tgctgaatca ttaattgctt atcattagcc
      301 atctttgttt caagttcgct agatgatcat tttattcaca tcctaacaaa cttgtcacat
      361 ttatagattc ttaatctatt tatcatacaa ttataaaaaa ggaggtgctg cttatgaaag
      421 ctatccatat ttctttgctt tttttcgcta gtgcattgat tttatttgct attcatcttt
      481 gttcccagcc ttatatcaat aacaacggaa tattagtgga accagcattc ttttgcttac
      541 cactcggctt tttgtccctt cttgcctccg caggcttcgg atttgttgct ttttttaaac
      601 gatcaaaaca acaaatctga cttataaaat ttacgtaaaa gagatcgtga caataatcgt
      661 gtgatattcc caaatttcga ataaaaggtg ccccttgcca tctctaactc ctcagtcaca
      721 agttacaata ttcataaacc atgaaaagct tgtttattga aattgctcct tgtgacttgg
      781 agctaagtga cagattacaa cctcttttgc atcataagac tttactcctt gaaaaacatt
      841 actaactttt gatatgtttc ttgcgcttgt ttattgctga aatcaaactg atattcatgg
      901 ttcaaaacag catttttctc cgcataatct acttttgtga ctttcacccc cgctttctca
      961 aatacagaag ttacttcttc catttgtttc gtaaacgtat ttgtatttcc atccgttaag
     1021 aatgtgtttt gcgtccaacg atccgcatgt ctgaccaagg ctaaactttt gaccttctct
     1081 gattgttcaa agtccttatt atcaaagtaa gcccatccac atttttcata aagaaagcta
     1141 gagattttat cacttacttg agtaagtgca gaaggatcca acagaccaga caaagaaata
     1201 aacttcgtaa tctgcttgtt ctccaaagtc ggatcaagat gatcagcttt tttaatttct
     1261 tcattttgaa gtgctaacaa atattctcca ctaatttgtg cacctgccga atttccacca
     1321 atcgaaatat tcgaccaatc tgcttgatct tttatctctt ttttagctgc ttgaatggct
     1381 tcgttcaatt ggataactgg tgtaggatgt tttgcttccg gtgatcgttc ataatctata
     1441 tttacgatga tttgctgcgt gtctgaacat agatattcca aataatcacg acaatcattt
     1501 ttatcccctc ctacatatgc gcctccatgc atccagaaaa gaatcggtaa ttttttcttt
     1561 tccactttcg gtgtgtagat atccatatta ttatttttgt actttgaagg ataggatatt
     1621 tcttttttta tatcgatttg ttcgattttt tgctggaaat catctggtcg ggcatatact
     1681 tgttcttttt cacttgtgtc aaattgtttt ctaacaagca agctaatcgg ctttggtgtg
     1741 atttgattaa tcaaaaatac agtgactaaa ataactacga tgataactgg tactgcaatc
     1801 actattcgct tgaaccattt cattcattta ccctctcttt cttcgccaaa tccatgtgat
     1861 aatcgtgcta acagataaaa atccacctga ataaatgaga agcgaatgtt tcttttctcc
     1921 agttttcggc aacattcttt tttttgcatc tgtctttttt tgtactttca ctgtcatctg
     1981 aatcggttgc gtatcatcac ttgattgatc ctctcgaggt tcattctttc ccttattgtc
     2041 tgcattctct gtcggatctt gcttttttgg tggagctact gggatttttt tgtaatagaa
     2101 aataatagtt ggtatcgtct cattaaaaat cccttcagtc ggtccttcaa catttgctaa
     2161 ttcgtatcct tcgaatgtct tttgctctgt catataattt tctccataag ctccgtaaag
     2221 ctggatttcg tcctggtctt tgagtagact tttattgttt tcatccacat actttacgtt
     2281 tatctgatca tggaacacta ctttcaaaaa gtccgttaca tgtttcgtgt aggcagcatg
     2341 gtcaacttcc tgtgcctttc catgccctgc tccttctact aataaaagat ctttataccc
     2401 tcctgataac tcatataatt tttgcgcgtt agacacaggt actacatcat ctgctgttcc
     2461 atggataatc aatttaggca atgaatctga cattccaatc gtaggtagag cagcttttgt
     2521 atccaaatgc atctcctgat caaaatagta ttgtttcaaa aggcccataa ataaatcttc
     2581 atgctcttga tccccaatga tgtctaactg attttttatg gcatccggta gtgcgggggc
     2641 tactgtctgg ctgtacgtaa aacgtagttg ttcatcaatt gatgcaaatc cgcaattttc
     2701 aataattcct ttgacattga caggaatatc tttcgctaat acagacatca tcgtagctgc
     2761 tcccatcgaa ccaccataaa gtaaaatctt ttgagatggc cgctctcgaa cttcttgatt
     2821 tatccagtaa aggacgtcat ctgactcata ttgtccatag gtcacataat cgcctccgct
     2881 attaccagtc gctctggaat caacaaataa taaattatac ccggccttat aaaaatcatt
     2941 gtattcttct gtaacgatcc cgttattcca attccctcga aatccgccat ggatcatgac
     3001 tgttttatct gaaccttgat cgacataata tgcatataca tccatcattt tgccatcttg
     3061 tttgtttagt acagtttgat gaagttgttt tgtatttgga tagaagcctg tgttttctgt
     3121 aggcgttgtc ccatcatctg tacctccaag catcgacatc aacacactgt tttttgcaaa
     3181 agcgatatcg tacaatagat aagtagcaat cagatgtttg ataatctctt tatctacacc
     3241 ataatcatta acagcaagct cagtgactaa taactctatt aattttgact tgatgtcttc
     3301 tgttggtcca ttaagtattt ctgtcatctg tttaccgaag taattcaaat attctttacc
     3361 attgaaattt tcacctttac cgatatcatc attttctgtc ccattctctt tgtcacttac
     3421 tttagcagaa gaggattcgt cgtttgacat ttttgtagct gttgcttctt ctgtttgttg
     3481 gttatctgtg gtagcggtcg tttcttgatc tgtgctagta acagatgtct caaccgttgt
     3541 ttcagtattt gtttctgttt gtgaagtact cgatttcgtt tcatttccag catcagtagt
     3601 ttctgtggtt tctacctccc gagattccga ttcggaagaa atgctctctc catatactgt
     3661 ccattgcggg acgcttgtac ttacagcaaa taagatgacc aacgctccct ttaatacttg
     3721 ttttgttctc aagctattca ctccttttta tgtttctagg agaattatat gtaaacgcaa
     3781 acatttttta tacaaaaaat aaatggttta tcccataaaa atagaacagg attattttct
     3841 cattttacgt aataaagcag aatggataaa ccagtatcca ttctgctttt tttaaacgaa
     3901 gaaacaactt attgctccaa aaatgtcaga aatcgtttat cccaaatttt ctcgtaaaaa
     3961 gcgattgttt cctttgcttt cataaaagga ttatcataag ttgtcgttgc tcctctacac
     4021 atcataagct ggtatcctaa tccatgagca aacttcacag tcgtatctat acaatactct
     4081 gcttgtgccc cacataattc gatcgaatga cagtccaatt cagttaataa aacactcaaa
     4141 ttagtcttat aaaatgaatt cgcatgggtt tttctcacaa aataactgtg ctcaggaata
     4201 gataattccg acaatagctc ccatggtttt gtttgtggca ctaattgagc atcttcgtgt
     4261 tgaacgaaaa taactggttt cccaatcgtt tcgtatgttt tgattctttt attcactaat
     4321 gctgtcactg tttctaattc gtgtatcggc gaagaatcaa aacatacccc tttttgtaaa
     4381 tcgatcacta ctattgcgtc ggctgtgttt gacatacttg tgccttcttt cttttttctt
     4441 gatttaatag tatttcttat ctagctagtt ccattatacg tgacaacatt ttcttatttt
     4501 acacctctaa atggcacttt tcgactattt cttcatgtta ttatttcata aatacttagc
     4561 tattggagga atgtatgatg attaaattgt atacaggttc tggctgctcg tcttcaagac
     4621 gcgcaaaacg ttggcttatc gtccatgata taccgtttat cgaacagaac ttaattcgtc
     4681 aaccactaac caaaaatgaa tttatccact tactaaaatt gacaaacaac ggcgtgaccg
     4741 aaatgctctc tactcattct attagttata aaaatcttgt tcacagaata gatgaactca
     4801 gtttgagtga actatatatt gagattcgta catccccttc tttattgaaa aaaccgatta
     4861 tctacgacca atctgcaaaa cttttgactg gatttagcga agataaaatc aggatgttcc
     4921 tttctaaaac taaaagaaag caagagctca acgaactctt gctttctggt catcttgtcg
     4981 attttaaaga aaataatctt tttgcaatgt cttaattttg gcataaacaa tctgcttttc
     5041 tggatctata atatctagtg ttggtgacgc cttctgttgc caatactttt tcttttttct
     5101 aataaacgca aacagacacc ttgaaatcct ttagaataaa gtcgacgaaa accattcaaa
     5161 ggagtgtttc aagatgtcct attcagagtt taccaaagag ttactagata ttctagacct
     5221 aaatcttacc tttcatgaag atgcctttcg caaagagcgc attaatgacg aaacatgttt
     5281 tgtttttgat ggcacattga cctatcaacc agaggaatgc ttccattgcc actatcaaaa
     5341 taagcaaacg atcattaaat ggggctggaa aaaagtatcc atcttattga atgatgtcag
     5401 taactacaag accattgtgc ggattaacaa acagcgcttc aaatgtaaac actgtggaaa
     5461 gactttttta gcagaagatt cagttagcga tcgtcgctgc tccattgccc gcagagtcaa
     5521 acaagccatt cttgagctat taagtgagcc tatttctatg tctttgattg cacgtatgaa
     5581 gcacatttct ccgaccactg tcattcgaat acttcgcagc cttcggccta aaaccgtttc
     5641 tttgaaccag ccgttaccag aagtggtgtg tttcgatgaa ttcaaatcgg tgaaaaacgt
     5701 ctcgggagcc atgagctttg tcatgatgga cggcaagacc catcaactga ttgatattgt
     5761 ggaaaatcgg caactcaatc ctttacgcga ctatttcttg cgctacccta gaaaagtccg
     5821 tgaacaagta cgtctagtgg tttcagattt ctatacaccc tatcgcacat tggtgaaaga
     5881 gtgttttccg aatgcccgaa tcgtcgccga tcgctttcat attagccaac acatcggtcg
     5941 ggcctttacc aaccaccgaa ttcaagtaat gaagaccttt aaaaaagggg atcgtcggtc
     6001 gaagcactta aagaaatact ggagactact tcaaaagaat gcgtgggaat tgaatggtca
     6061 gcatcgctac tggcgtccgt catttcgaga tcatctgaca gaagcggaga tagtcgatcg
     6121 gctattatat tatgatgact ccttaaagcg aggctacgaa gtctaccagg tttttctttc
     6181 cgctatcaga agacaagatg ttcctgaatt cgtcgcgctc ctaaaagaag actacaagga
     6241 gttgccagag cactatcaac cagtgttcac gaccttcaag aaatatcgaa cagaaatcaa
     6301 acgagcgttg cgagttccct attccaacgg ccctatagaa tgtttgaaca accatatcaa
     6361 agtactaaaa cgaattgcct atggctttag aaactttcaa aactaccgag aacgaatctt
     6421 tttatatcga ggaaaatatt tcaagaaaac taagaa
//
LOCUS       DAFCNJ010000096         6303 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000096 DAFCNJ010000000
VERSION     DAFCNJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.101"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            334..714
                     /locus_tag="LNE35_002667"
     CDS             334..714
                     /locus_tag="LNE35_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBK6911762.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="LNE35_002668"
     CDS             696..1610
                     /locus_tag="LNE35_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK6911763.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="LNE35_002669"
     CDS             1855..2544
                     /locus_tag="LNE35_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911764.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="LNE35_002670"
     CDS             complement(2665..2751)
                     /locus_tag="LNE35_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK6911765.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="LNE35_002671"
     CDS             3427..4377
                     /locus_tag="LNE35_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBK6911766.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="LNE35_002672"
     CDS             4398..4931
                     /locus_tag="LNE35_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK6911767.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="LNE35_002673"
     CDS             4947..5117
                     /locus_tag="LNE35_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911768.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="LNE35_002674"
     CDS             5567..6100
                     /locus_tag="LNE35_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911769.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAFCNJ010000097         6287 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000097 DAFCNJ010000000
VERSION     DAFCNJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.102"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..177
                     /locus_tag="LNE35_002675"
     CDS             <1..177
                     /locus_tag="LNE35_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911770.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="LNE35_002676"
     CDS             190..690
                     /locus_tag="LNE35_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK6911771.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="LNE35_002677"
     CDS             787..2634
                     /locus_tag="LNE35_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBK6911772.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="LNE35_002678"
     CDS             2637..3533
                     /locus_tag="LNE35_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6911773.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="LNE35_002679"
     CDS             3583..3972
                     /locus_tag="LNE35_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6911774.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="LNE35_002680"
     CDS             3959..>6287
                     /locus_tag="LNE35_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6911775.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAFCNJ010000098         6173 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000098 DAFCNJ010000000
VERSION     DAFCNJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.103"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            725..811
                     /locus_tag="LNE35_002681"
     CDS             725..811
                     /locus_tag="LNE35_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK6911776.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="LNE35_002682"
     CDS             complement(932..1621)
                     /locus_tag="LNE35_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911777.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="LNE35_002683"
     CDS             complement(1628..1906)
                     /locus_tag="LNE35_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911778.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="LNE35_002684"
     CDS             complement(1866..2780)
                     /locus_tag="LNE35_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK6911779.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="LNE35_002685"
     CDS             complement(2762..3142)
                     /locus_tag="LNE35_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBK6911780.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="LNE35_002686"
     CDS             complement(3592..3879)
                     /locus_tag="LNE35_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6911781.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="LNE35_002687"
     CDS             complement(3899..4123)
                     /locus_tag="LNE35_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBK6911782.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="LNE35_002688"
     CDS             complement(4578..4748)
                     /locus_tag="LNE35_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911783.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="LNE35_002689"
     CDS             complement(4763..5296)
                     /locus_tag="LNE35_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK6911784.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="LNE35_002690"
     CDS             complement(5317..6159)
                     /locus_tag="LNE35_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6911785.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAFCNJ010000099         6133 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000099 DAFCNJ010000000
VERSION     DAFCNJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.104"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(207..1169)
                     /locus_tag="LNE35_002691"
     CDS             complement(207..1169)
                     /locus_tag="LNE35_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6911786.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="LNE35_002692"
     CDS             complement(1171..2610)
                     /locus_tag="LNE35_002692"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBK6911787.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="LNE35_002693"
     CDS             2795..4741
                     /locus_tag="LNE35_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBK6911788.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="LNE35_002694"
     CDS             5005..5877
                     /locus_tag="LNE35_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6911789.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1954 a         1056 c         1080 g         2043 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aat
//
LOCUS       DAFCNJ010000100         5924 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000100 DAFCNJ010000000
VERSION     DAFCNJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.105"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(75..1169)
                     /locus_tag="LNE35_002695"
     CDS             complement(75..1169)
                     /locus_tag="LNE35_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK6911790.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="LNE35_002696"
     CDS             1358..2119
                     /locus_tag="LNE35_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6911791.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="LNE35_002697"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="LNE35_002697"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK6911792.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2894..3649
                     /gene="fabI"
                     /locus_tag="LNE35_002698"
     CDS             2894..3649
                     /gene="fabI"
                     /locus_tag="LNE35_002698"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBK6911793.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3759..4772)
                     /locus_tag="LNE35_002699"
     CDS             complement(3759..4772)
                     /locus_tag="LNE35_002699"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBK6911794.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4937..5857
                     /locus_tag="LNE35_002700"
     CDS             4937..5857
                     /locus_tag="LNE35_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBK6911795.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1180 c         1063 g         1875 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag caatcttttt
     2761 ttaatttaag aaaaaatagt ttgacaatca aaacattttg actaataatt aagaaatcaa
     2821 gaagttattt tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca
     2881 aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa
     2941 caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat
     3001 ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc
     3061 attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat
     3121 cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga
     3181 agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat
     3241 cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag
     3301 cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat
     3361 gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc
     3421 taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg
     3481 tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg
     3541 tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg
     3601 aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc
     3661 attctttaaa gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt
     3721 ttttcttttt acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag
     3781 ccttctggtg cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt
     3841 gttactggtc ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc
     3901 ccaccgattg gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct
     3961 agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac
     4021 tgcaacaagg aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat
     4081 tttccgtctt ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt
     4141 gtggctggat caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga
     4201 agtaaactcg ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca
     4261 cgatcgtaat cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct
     4321 actggcgtac agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca
     4381 ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca
     4441 aaatcttcat ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc
     4501 attggcatca gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga
     4561 aagagattga ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga
     4621 ccataatgaa gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct
     4681 ccttcttccc attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa
     4741 gaaaaaccta gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca
     4801 cattttatca ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag
     4861 ttatttttgc tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt
     4921 tagaaaagag gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg
     4981 cgagacattg acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg
     5041 gattgattgg gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt
     5101 gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa
     5161 tcagtattct aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt
     5221 aaatactatt tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat
     5281 cggtgtgccg atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct
     5341 tggtgcccaa gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca
     5401 gatggatgtt ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc
     5461 tgttggtatc cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa
     5521 tcgaaacatc accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat
     5581 tcggattgtt cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg
     5641 tacattagct gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt
     5701 ggatcttgga aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca
     5761 atatgcctta aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt
     5821 cacgattccg aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat
     5881 ctcactaaaa agagcgtggg acaaaagtaa agaacacttt tgtc
//
LOCUS       DAFCNJ010000101         5859 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000101 DAFCNJ010000000
VERSION     DAFCNJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.106"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..395
                     /locus_tag="LNE35_002701"
     CDS             <1..395
                     /locus_tag="LNE35_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911796.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="LNE35_002702"
     CDS             398..1228
                     /locus_tag="LNE35_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBK6911797.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="LNE35_002703"
     CDS             1244..2095
                     /locus_tag="LNE35_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6911798.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="LNE35_002704"
     CDS             2092..2451
                     /locus_tag="LNE35_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBK6911799.1"
                     /translation="MNKYRNKKTVHRGIKSDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="LNE35_002705"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="LNE35_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="LNE35_002706"
     CDS             2624..2929
                     /locus_tag="LNE35_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911800.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="LNE35_002707"
     CDS             2929..3240
                     /locus_tag="LNE35_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBK6911801.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="LNE35_002708"
     CDS             3237..3410
                     /locus_tag="LNE35_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911802.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="LNE35_002709"
     CDS             3407..3823
                     /locus_tag="LNE35_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBK6911803.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="LNE35_002710"
     CDS             3823..4491
                     /locus_tag="LNE35_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBK6911804.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="LNE35_002711"
     CDS             4488..4865
                     /locus_tag="LNE35_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911805.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="LNE35_002712"
     CDS             4862..5161
                     /locus_tag="LNE35_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911806.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="LNE35_002713"
     CDS             5151..5309
                     /locus_tag="LNE35_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911807.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="LNE35_002714"
     CDS             5403..5819
                     /locus_tag="LNE35_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK6911808.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2175 a          886 c         1361 g         1437 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtctg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgag
//
LOCUS       DAFCNJ010000102         5809 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000102 DAFCNJ010000000
VERSION     DAFCNJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.107"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            450..1604
                     /locus_tag="LNE35_002715"
     CDS             450..1604
                     /locus_tag="LNE35_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBK6911809.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="LNE35_002716"
     CDS             1598..2020
                     /locus_tag="LNE35_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911810.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="LNE35_002717"
     CDS             3143..4075
                     /locus_tag="LNE35_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6911811.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="LNE35_002718"
     CDS             4092..4607
                     /locus_tag="LNE35_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK6911812.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="LNE35_002719"
     CDS             4903..5184
                     /locus_tag="LNE35_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911813.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="LNE35_002720"
     CDS             5186..5602
                     /locus_tag="LNE35_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911814.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAFCNJ010000103         5790 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000103 DAFCNJ010000000
VERSION     DAFCNJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.108"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="LNE35_002721"
     CDS             complement(99..1229)
                     /locus_tag="LNE35_002721"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBK6911815.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="LNE35_002722"
     CDS             complement(1877..3079)
                     /locus_tag="LNE35_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6911816.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="LNE35_002723"
     CDS             complement(3139..3342)
                     /locus_tag="LNE35_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBK6911817.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="LNE35_002724"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="LNE35_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="LNE35_002725"
     CDS             4879..5691
                     /locus_tag="LNE35_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6911818.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAFCNJ010000104         5611 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000104 DAFCNJ010000000
VERSION     DAFCNJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.110"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..2008)
                     /locus_tag="LNE35_002726"
     CDS             complement(59..2008)
                     /locus_tag="LNE35_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBK6911819.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="LNE35_002727"
     CDS             complement(2050..3108)
                     /locus_tag="LNE35_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HBK6911820.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="LNE35_002728"
     CDS             complement(3227..3682)
                     /locus_tag="LNE35_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911821.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="LNE35_002729"
     CDS             complement(4266..5069)
                     /locus_tag="LNE35_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6911822.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2073 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat a
//
LOCUS       DAFCNJ010000105         5532 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000105 DAFCNJ010000000
VERSION     DAFCNJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.111"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..985)
                     /locus_tag="LNE35_002730"
     CDS             complement(<1..985)
                     /locus_tag="LNE35_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HBK6911823.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="LNE35_002731"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="LNE35_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK6911824.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="LNE35_002732"
     CDS             1810..2964
                     /locus_tag="LNE35_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK6911825.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="LNE35_002733"
     CDS             3179..4147
                     /locus_tag="LNE35_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK6911826.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="LNE35_002734"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="LNE35_002734"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK6911827.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5532
                     /locus_tag="LNE35_002735"
     CDS             5177..>5532
                     /locus_tag="LNE35_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HBK6911828.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1669 a         1078 c         1252 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc gg
//
LOCUS       DAFCNJ010000106         5340 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000106 DAFCNJ010000000
VERSION     DAFCNJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.112"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(61..279)
                     /locus_tag="LNE35_002736"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="LNE35_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="LNE35_002737"
     CDS             complement(545..739)
                     /locus_tag="LNE35_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911829.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="LNE35_002738"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="LNE35_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="LNE35_002739"
     CDS             2091..3131
                     /locus_tag="LNE35_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK6911830.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3955..4287
                     /locus_tag="LNE35_002740"
     CDS             3955..4287
                     /locus_tag="LNE35_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK6911831.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4906..5247
                     /locus_tag="LNE35_002741"
     CDS             4906..5247
                     /locus_tag="LNE35_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBK6911832.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1769 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     4801 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat
     4861 ataaaacata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     4921 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     4981 ggatttgtga cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac
     5041 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     5101 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     5161 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     5221 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     5281 tagaaggagt cacgatgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
//
LOCUS       DAFCNJ010000107         5247 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000107 DAFCNJ010000000
VERSION     DAFCNJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.113"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1389
                     /gene="gadC"
                     /locus_tag="LNE35_002742"
     CDS             <1..1389
                     /gene="gadC"
                     /locus_tag="LNE35_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HBK6911833.1"
                     /translation="GGILWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAI
                     FFQWFQITVGFITMIYFILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTN
                     RTAKIAKAGFVIGIVIPSIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFV
                     SFILAYMGVEASASHINELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLS
                     LSAGVIQTFETLILHFNSHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGL
                     LPVFFRKTNKQDVPVPLIMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGY
                     LLFFLGYFVLVFKKKDLKRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAAS
                     EHRTYQIILLISFVVTVLLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEH
                     HVTKNDEHILKH"
     gene            1402..2802
                     /locus_tag="LNE35_002743"
     CDS             1402..2802
                     /locus_tag="LNE35_002743"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBK6911834.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(2967..4407)
                     /locus_tag="LNE35_002744"
                     /pseudo
     CDS             complement(2967..4407)
                     /locus_tag="LNE35_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4800..5247
                     /locus_tag="LNE35_002745"
                     /pseudo
     CDS             4800..5247
                     /locus_tag="LNE35_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT         1675 a          850 c         1001 g         1721 t
ORIGIN      
        1 ggaggtattt tatggtttct accagttgct ttatgtgctg cagaaatggc aacagttgaa
       61 ggatggaaag atggtgggat tttcagttgg gttagtcaaa ctttaggaga acgttttggt
      121 tttgcagcta tttttttcca atggtttcaa attacagtag gttttattac aatgatctac
      181 ttcatactag gagcgctgtc gtatgtaatt aattttccag agcttaatac agatccattt
      241 atcaagttta ttggtgtatt agtcattttc tgggtactga ctttttcaca gttgggcggg
      301 accaatcgta ccgctaaaat tgctaaagca ggatttgtca ttggaattgt tattccttcg
      361 atcattctct ttggtttagc agcagcctac tttatcggcg gtaatccgat tcaaatggct
      421 attaatgcga aagcttttat tcctgacttt tctcagcttt caactttagt agtatttgta
      481 tcttttatat tggcttatat gggcgtagaa gcttcagcat cacatattaa tgagctggaa
      541 aatccccaaa gaaactaccc attggcaatg tttttactag tgattttggc catcgcctta
      601 gatgcaatcg gcggtttctc agtagcagcg gttattccac aacatgactt gtctctcagt
      661 gctggtgtaa tccaaacatt tgaaacgctt attttacatt ttaacagtca tttacattgg
      721 ttggtaaaag tgattgctct catgattgcc tgtggcgtta tgggtgaagt tagttcttgg
      781 gtagttggtc catcacgagg gatgtttgca gctgctcagc gaggtctgtt accagttttc
      841 ttccggaaaa ctaataaaca agacgttcca gttccgttga ttatgataca aggaattatc
      901 gttactattt gggcagcagt cttaactttt ggtggaggag gcaacaattt atcattctta
      961 gtagcaatct ccttaactgt tgtcatttat ctcgtaggat atttactatt tttcctaggt
     1021 tactttgtat tagtttttaa gaaaaaagac ttaaaacgaa cttacaatgt accaggaaaa
     1081 actgttggca aaacgattat cggtggaatt ggttttattt tatcagtgtt cgcattgctt
     1141 atctcttttg taccaccagc ttcaattgca gctagtgagc accgaacata ccaaatcata
     1201 ctacttatta gctttgtagt gactgttctt ttacctttta tcatttatga agtacatgat
     1261 aaaagtgctc acacaactat tgaggaacct actcacgtga aagctgaaga cgttaatccc
     1321 gctgtttatc cagctgctag aggagagcac cacgttacaa aaaatgatga acatatttta
     1381 aaacattagg aggaattcat tatgttatac ggaaaagata atcaagaaga aaaaaactat
     1441 ttggaaccaa tttttggctc tgcaagtgag gatgttgact tgcctaaata taagttaaac
     1501 aaagaatcca ttgaaccacg aattgcttat caattagtac aagacgagat gttggatgaa
     1561 ggaaatgcgc gattaaactt agctactttt tgtcaaacgt atatggaacc tgaagcagtg
     1621 aaattgatga cccaaacgtt agaaaaaaat gcaattgata aatcagaata cccacgaaca
     1681 acggaaattg aaaaccgctg tgtaaatatg attgctgatt tatggcatgc tccaaataat
     1741 gaaaaattca tgggaacttc aacgatcggc tcttcagaag cctgcatgct gggtggtatg
     1801 gccatgaaat ttgcttggcg taaacgtgct gaaaaattag gtcttgatat tcaagcaaaa
     1861 aaacctaacc tggtgatctc ttctggttac caagtttgtt gggaaaaatt ctgtgtatat
     1921 tgggatgtgg aactgagaga agtcccaatg gatgaaaaac atatgtcaat taatctagat
     1981 actgtcatgg attatgtgga tgagtacaca attggtattg taggtattat gggtattact
     2041 tacactggtc gttatgatga tatcaagggt ctgaatgatt tagttgaagc tcacaataaa
     2101 caaactgact ataaagtata cattcatgtt gacgctgcat cgggtggctt ttatgcacca
     2161 tttactgaac ctgatctagt ttgggatttt caattgaaaa atgttatctc aattaattct
     2221 tcaggtcaca aatatggttt ggtatatcca ggtgtgggtt gggtcttatg gcgtgaccaa
     2281 caatacttac cagaagaatt agtatttaaa gttagttact taggtggaga aatgccaact
     2341 atggctatca acttctctca tagtgcagca caactaattg ggcaatacta caactttgta
     2401 cgctatggct ttgatggtta tcgtgatatt caccaaagaa ctcatgatgt tgctgtttat
     2461 ttagccaaag agatcgaaaa aactggtatt tttgaaatca ttaatgatgg atcagaattg
     2521 ccagttgtgt gctataagct gaaagaagat cccaatcgcg aatggacact atatgattta
     2581 tctgatcgtc tgttaatgaa gggatggcaa gtcccagcct acccattacc taaagacttg
     2641 gatcaattaa ttattcaacg cttagttgtt cgagcagact ttggaatgaa catggctggt
     2701 gattatgtac aagatatgaa ccaagcaatt gaagagttga ataaagctca tattgtttat
     2761 cataaaaaac aggatgtgaa aacgtacggc tttactcact aaaattcttc agagaaatat
     2821 aaaagaagtt aattaactaa taaaaggagt tgtgattacc acacagctcc ttaaatctat
     2881 ttgaataaat atatgatatt catttgttac actctcaatc ttagctgttg ttctattcat
     2941 cattactctt attgtaagta gttcagtcaa atttgcttgt tcaattgatc gataacagta
     3001 tgaattgttt gaaaagtaat cggatatata attgttttct cactacctaa gccttcttgg
     3061 tataaagaac tatcgcaaag tataatccgc tgagtacaaa agaaagtgtc gtctactatt
     3121 tctctaagtt gtttacaaat gatattacta atagaaaagt gttctcccaa aatgtcttct
     3181 aactgttgtc gatagtataa atacctaaag tctgacggag ctactatgaa cacttcaatt
     3241 ggagatttcc gtttatgctc atttaaaaga cttaaactaa tggttagttt acgaatagaa
     3301 ttctcattcc aacgcagaaa taaattgttt tgttttcgcc attgttcaag aatttcatga
     3361 acttctaagt acaaagcgcg ttgctcttca tttaacaagt aagtcttatc aggttgaaat
     3421 atctgaatat cagcccaaag tgagcggata aacgggagaa gtgctttatc aaaaagtatc
     3481 tgattttctt cagatatatt cagagataag ataagttttt gactgagctc aatcaccaaa
     3541 gggttatctg ctacatggtt ttgataaaca acgttcaagt ctttttgtaa caattcaaag
     3601 ttatcgcata gataatttct agagttgaat agaaagtaga tataaaggca ttcatcttcg
     3661 tcatgacata aagagaaaga aagttgctct aatcctttct gtattagctg aaacaaggga
     3721 gtatgtcggg caactctttt ttcttcctga ctaaaatata ttgggttagt cttagatctc
     3781 tgcaaaccga tagcgatacc taataaaata aattgttttt caactgtagg gtaacttctt
     3841 gaaaaaaaat gtttttctac ataatcaatc aattgttgac cgctttgaat aattgattta
     3901 tttagctgga tcaactctgt ccgatttgtc tcatagatta tcgttagtaa caaacttcga
     3961 taatcctttt caggaatttc aaaatggcca tcagcattta agtactgcat ttgtttaaaa
     4021 aaattaatca actcatttcg taagttgtaa actttgctta acgaaatgaa ggtagtatca
     4081 gctatattac tccaattgtg ttcccctgat aaataatgtc ctagtacatt gaaaaagtca
     4141 gagcgctgat agatgagttg agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact
     4201 gtaaagctac ttctagattt ctccactgaa ataatttgct gatggagtat gtcggtaatg
     4261 cattccaagt cgttagtgac agacagtgag tgggcaaacg ttcaattgat ctgctatttc
     4321 gcgaatctga atggtccctt tttcgtgtaa ttgctcacaa atgtaaactt gacgaaaaat
     4381 acttttctcc agataggttt ccaacatagt actcctccta gtcattttat acataactat
     4441 tataactcta ccaatcggaa tagtcctgta atatattgaa tcaaaaatat aaattataaa
     4501 ataattagat ggggtatttg gtttaagtat cccttatata gtaataaatt tataggtgtg
     4561 attctcgaat taatacccga aaatgtgaga gaatttttat ggatttttta ggatacaaat
     4621 taattatttt tgcttttatc ctgattacca gtccagtact gtcggtatcc cattttgcag
     4681 caaaatcatg ttgataaagt gacaagaaga ctgatgagat atattatcca aattgagaag
     4741 ataattaatc ggattcttga gtatacccta ctttttaaga aagtagggta tacaagcgta
     4801 tgaataaaga cataactttc cctgtgatgg acgttcggct tgttccaatt accaaaatac
     4861 aggcgaacga ttataatccc aatcgagtag cgtctccgga aatgaactta ttggagcttt
     4921 ctatcattga agatggcttt actcaaccat tagtttgtta ttacaaggaa aaagaagaga
     4981 agtatatctt ggttgatgtg cttcataggt atcgtgttgc taaggaaaga ctcaacttaa
     5041 aagttgtacc ggtaacagtt attgataaac ccatataaga acgtatggct tcaacaattc
     5101 gacacaatcg tgcaagaggg acccatgaaa taagaggaat ggaaattgtc atccaaagat
     5161 tagtaaaaaa tggatggtca gacaagagaa tatccaaaga attaggcatg gacattgaag
     5221 aaatttttcg ctttaagcaa gtgtctg
//
LOCUS       DAFCNJ010000108         5220 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000108 DAFCNJ010000000
VERSION     DAFCNJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.114"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1256)
                     /locus_tag="LNE35_002746"
     CDS             complement(<1..1256)
                     /locus_tag="LNE35_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBK6911835.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="LNE35_002747"
     CDS             complement(1270..2487)
                     /locus_tag="LNE35_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBK6911836.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="LNE35_002748"
     CDS             complement(2477..4072)
                     /locus_tag="LNE35_002748"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBK6911837.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="LNE35_002749"
     CDS             4197..4982
                     /locus_tag="LNE35_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HBK6911838.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DAFCNJ010000109         4788 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000109 DAFCNJ010000000
VERSION     DAFCNJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.115"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            564..989
                     /locus_tag="LNE35_002750"
     CDS             564..989
                     /locus_tag="LNE35_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911839.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="LNE35_002751"
     CDS             1019..1183
                     /locus_tag="LNE35_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911840.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="LNE35_002752"
     CDS             2141..3118
                     /locus_tag="LNE35_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6911841.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="LNE35_002753"
     CDS             3128..3625
                     /locus_tag="LNE35_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6911842.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="LNE35_002754"
     CDS             4001..4282
                     /locus_tag="LNE35_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911843.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="LNE35_002755"
     CDS             4284..4700
                     /locus_tag="LNE35_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911844.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAFCNJ010000110         4720 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000110 DAFCNJ010000000
VERSION     DAFCNJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.116"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1097)
                     /locus_tag="LNE35_002756"
     CDS             complement(<1..1097)
                     /locus_tag="LNE35_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK6911845.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="LNE35_002757"
     CDS             complement(1227..2603)
                     /locus_tag="LNE35_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6911846.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="LNE35_002758"
     CDS             complement(2603..3259)
                     /locus_tag="LNE35_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6911847.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="LNE35_002759"
     CDS             complement(3269..4546)
                     /locus_tag="LNE35_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6911848.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="LNE35_002760"
     CDS             complement(4565..>4720)
                     /locus_tag="LNE35_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBK6911849.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          858 c          566 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DAFCNJ010000111         4719 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000111 DAFCNJ010000000
VERSION     DAFCNJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.117"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..354)
                     /locus_tag="LNE35_002761"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="LNE35_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="LNE35_002762"
     CDS             complement(614..1162)
                     /locus_tag="LNE35_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911850.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="LNE35_002763"
     CDS             complement(1163..2020)
                     /locus_tag="LNE35_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6911851.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2594)
                     /locus_tag="LNE35_002764"
                     /pseudo
     CDS             complement(2306..2594)
                     /locus_tag="LNE35_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2584..2913)
                     /locus_tag="LNE35_002765"
     CDS             complement(2584..2913)
                     /locus_tag="LNE35_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6911852.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            3091..3660
                     /locus_tag="LNE35_002766"
                     /pseudo
     CDS             3091..3660
                     /locus_tag="LNE35_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
     gene            3795..4121
                     /locus_tag="LNE35_002767"
     CDS             3795..4121
                     /locus_tag="LNE35_002767"
                     /inference="COORDINATES: protein motif:HMM:NF012464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6911853.1"
                     /translation="MKYGYARVSTRHQDLEGQMRQLEEERCDKIFFEKITGTKSDRPE
                     FQKLLQEVQTGDTLVVTKLDRFARSTQDALHTIKLLFEKGVRINVLNLGIIENTSTGR
                     LIIHYF"
     gene            4153..4347
                     /locus_tag="LNE35_002768"
     CDS             4153..4347
                     /locus_tag="LNE35_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBK6911854.1"
                     /translation="MERTQEGKEIAKQRPGFREGRPKKFSKQQMTLAMKLLETNSYKE
                     VEKMTGISKSTLTRYKRKKS"
     gene            4499..>4719
                     /locus_tag="LNE35_002769"
     CDS             4499..>4719
                     /locus_tag="LNE35_002769"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6911855.1"
                     /translation="MFQSFIHEKQRELSIKMIQALKTFRQSEEAIVNALNYHYSNGLV
                     EGINNKIKVIKRTAYGYRNFSNFRNRIFI"
BASE COUNT         1438 a          905 c          738 g         1638 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccagtc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ccccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagg aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
     2521 tctttcaatg gcttctcttt tagctttttt aaatacttaa tgacatgttt tgaaggaacg
     2581 atttcataag ccaatttctg ttgccaactc ctctttagtc attactttag aattagtaag
     2641 tgtatcttca gcaatatctc taaaggaaga atgcattttg ttttttctat tcttaaattc
     2701 tacaagaatc tcatctttat cgtagccttc tgcaattata tcagacaaaa tatcttcgct
     2761 aaaatcataa aattcttttt gtggttcaac aaattcataa agaataccat tctctaccaa
     2821 ctttgcttca acttcgactc cattaggaac attaaaatct ttaggtactg tcaacatgat
     2881 tgaattgcct tgttttcttg tttttgtaat catttaaatc cctcatttct aacagtatgt
     2941 aattacttag taaatacatt ataccacgag ataatgtatt tacaagtaat tactgtgact
     3001 cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc atcttcatct accttatttg
     3061 atagtagatt taaaactttg caacagaacc attgtagcta ttggggcaaa gacttattca
     3121 gctcactcga ttgccggcag cattgaaagt tttgtttata tgccagctta tggattagct
     3181 accgctgcag caactttagc aggaaatagt attggaaaaa aagactatgt ggaaacaaaa
     3241 cgtgttgcat tttatgcggt taaatacggc gtgattgttt taagtatcct aggtatagga
     3301 ttgttttttg gtacgccata tattgcacct tggtttacca cagatgctga ggcaataaag
     3361 caaattgtta ttgcgctaag aattgacgct tttaatcaac caggactagc tatcagtcta
     3421 atattagctg gcgtactcca aggaatggga gatactaaaa ccccattata cagtactgca
     3481 tttggaatgt gggtcacacg catattaggt gtaatacttt tagggaatat attggattta
     3541 gggattgctg gagtgtggtt agcaattggc atggatctat atatacgttc acttttttta
     3601 atctatcgtt tcagacgaaa tatccaaatt ttaaataaca ataacgaaac accttcctaa
     3661 accagtgatt ttttatcgct ggtttttgcc atttagcgat taaaaaaatt gccatataaa
     3721 tagaaaagta acacaagggg tacaatggtg tcataactaa tggtcataag tgaagtgaaa
     3781 taaggggtga aaacatgaaa tatggctatg cacgggtgag tacccgccac caagatctgg
     3841 aagggcaaat gcgtcagctg gaagaggaac gctgtgataa aattttcttt gaaaaaataa
     3901 cgggaacgaa gagtgatcgt ccagaattcc aaaaactgct tcaggaagtt cagactggtg
     3961 atacgttagt ggtcacaaaa ttagaccgat ttgctcgtag cacgcaggat gctcttcata
     4021 caatcaagct tctttttgaa aaaggcgtgc gaattaatgt tttgaatttg ggcattattg
     4081 agaacacgtc tactgggcga ttaattattc actattttta gtgcctttgc ggactttgaa
     4141 cgcgatttaa ttgtggaacg gacacaagag ggaaaagaaa ttgccaagca gcggcccggt
     4201 tttagagaag ggcgaccaaa aaaattttcc aaacaacaga tgactttagc aatgaaacta
     4261 ctagagacaa attcctataa agaagtagag aaaatgactg gtattagcaa aagtacttta
     4321 acgcgttata aaaggaagaa gagttaggag ccacagaata ccgatatcat cgtcttttca
     4381 aaggcatggt gacggaaagg ggaatcgttg attattttct ttccttggac gaaggtttac
     4441 gccgccatta caacgtctac caaacaactg tctttgccat taactatcgg aaccgaaatt
     4501 gtttcagtcc ttcatccatg aaaagcaacg ggagctttca attaagatga ttcaagcctt
     4561 aaaaactttc cgacaatcag aggaagccat tgtcaatgcg ttaaattatc actactccaa
     4621 tggtttagtg gaaggtatca ataataaaat caaagttatt aaacggacag cctatggcta
     4681 ccgcaacttt tccaattttc gtaatcgtat ctttattga
//
LOCUS       DAFCNJ010000112         4710 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000112 DAFCNJ010000000
VERSION     DAFCNJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.118"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            106..357
                     /locus_tag="LNE35_002770"
                     /pseudo
     CDS             106..357
                     /locus_tag="LNE35_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="LNE35_002771"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="LNE35_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBK6911856.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="LNE35_002772"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="LNE35_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="LNE35_002773"
     CDS             1786..1869
                     /locus_tag="LNE35_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBK6911857.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="LNE35_002774"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="LNE35_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBK6911858.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="LNE35_002775"
     CDS             complement(2994..3857)
                     /locus_tag="LNE35_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HBK6911859.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="LNE35_002776"
     CDS             complement(3859..4131)
                     /locus_tag="LNE35_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK6911860.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="LNE35_002777"
     CDS             complement(4148..4357)
                     /locus_tag="LNE35_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBK6911861.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="LNE35_002778"
     CDS             complement(4455..>4710)
                     /locus_tag="LNE35_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HBK6911862.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DAFCNJ010000113         4646 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000113 DAFCNJ010000000
VERSION     DAFCNJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.119"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..717)
                     /locus_tag="LNE35_002779"
     CDS             complement(133..717)
                     /locus_tag="LNE35_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6911863.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="LNE35_002780"
     CDS             complement(928..1131)
                     /locus_tag="LNE35_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911864.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="LNE35_002781"
     CDS             complement(1141..1563)
                     /locus_tag="LNE35_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911865.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="LNE35_002782"
     CDS             1801..2415
                     /locus_tag="LNE35_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6911866.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="LNE35_002783"
     CDS             complement(2580..3404)
                     /locus_tag="LNE35_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911867.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="LNE35_002784"
     CDS             complement(3428..3583)
                     /locus_tag="LNE35_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911868.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="LNE35_002785"
     CDS             complement(4042..4299)
                     /locus_tag="LNE35_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK6911869.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="LNE35_002786"
     CDS             complement(4292..4561)
                     /locus_tag="LNE35_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6911870.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttat
//
LOCUS       DAFCNJ010000114         4632 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000114 DAFCNJ010000000
VERSION     DAFCNJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.120"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            174..518
                     /locus_tag="LNE35_002787"
     CDS             174..518
                     /locus_tag="LNE35_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBK6911871.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="LNE35_002788"
     CDS             519..938
                     /locus_tag="LNE35_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBK6911872.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="LNE35_002789"
     CDS             complement(1030..1380)
                     /locus_tag="LNE35_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911873.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="LNE35_002790"
     CDS             complement(1346..2566)
                     /locus_tag="LNE35_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBK6911874.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="LNE35_002791"
     CDS             2932..3498
                     /locus_tag="LNE35_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6911875.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="LNE35_002792"
     tRNA            3643..3730
                     /locus_tag="LNE35_002792"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4632
                     /locus_tag="LNE35_002793"
     CDS             4064..>4632
                     /locus_tag="LNE35_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBK6911876.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1682 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat gg
//
LOCUS       DAFCNJ010000115         4609 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000115 DAFCNJ010000000
VERSION     DAFCNJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.121"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(84..338)
                     /locus_tag="LNE35_002794"
     CDS             complement(84..338)
                     /locus_tag="LNE35_002794"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6911877.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(449..703)
                     /locus_tag="LNE35_002795"
     CDS             complement(449..703)
                     /locus_tag="LNE35_002795"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6911878.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(896..1171)
                     /locus_tag="LNE35_002796"
     CDS             complement(896..1171)
                     /locus_tag="LNE35_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBK6911879.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1143..2096)
                     /locus_tag="LNE35_002797"
     CDS             complement(1143..2096)
                     /locus_tag="LNE35_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6911880.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2708..4201
                     /locus_tag="LNE35_002798"
     CDS             2708..4201
                     /locus_tag="LNE35_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBK6911881.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4336..>4609
                     /locus_tag="LNE35_002799"
     CDS             4336..>4609
                     /locus_tag="LNE35_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBK6911882.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1541 t
ORIGIN      
        1 gcttgaattc ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta
       61 tgttttcttc tagtctacac accttaattt ttctgtctag ttaattgtag cctatccatg
      121 atcggtttat tagacctttg aggtctatat tttttaacta caatcgacct taaatttaat
      181 tgtttcatta gcttttggac atgtttaaga gatactgaaa tcccttcttt taataaaact
      241 tgatggatct ttgtggcacc atagcgttgt ttattcgtaa aaaaaattgt tgaaatcttc
      301 tttttcaact tattatttct gatttctgta gctgtcaacg gtttattctt ataaaaataa
      361 tagacacttc tagggatttc taataaccga cacaacaaag aaacagtgta atccttacac
      421 catttttcaa taaattgaac aagcgaattt aggttttccg agagaatatg gctgcggctt
      481 tttttaagat ttcaagttcc tcatttaaac gagcattttc ttttctcagt tcagctactt
      541 cttttccagt cagtcctgtg gactgatcag gtaaatataa attcttccat ttgtaaatcg
      601 ttgcttcaga taagccatat tctttcgcta accctcgaac agaacgtcca gtttgattca
      661 gttcaacaat catttgttta aaattttctt catatcgggt cataaaaatt catcctctcg
      721 tagataagtt taatttattc ttgtctctac gaaaagctgt ctaagatttg atactaacac
      781 caaatgttgt atacccccaa acaatacaca tattttgaag gattaggatt aataaagcta
      841 aaaatatagt aagtatacta aatatattta ctatatctag tatacttact atattttaat
      901 cttttgatcg tctattatat ataatccgtt cctcgtcgtt taaagtatca acataggcgt
      961 ccattgcaag tttgactatt tcatacatag gaatatcttt tgcataacta agatcccgaa
     1021 tggtttttag tacgggagga tctacttgaa ctggttttct ttctttcgca gtgtactttc
     1081 gttcccctaa ctcgacatta atatcatctt taattgaaat atcttcagca ccaattttct
     1141 tcttatcttt gttcgttggt aataatgcca tttagtttaa tctcctttcc aagaggtagg
     1201 atatgattgg ccattgagtc agcatattta ggtgtataat caaatccttc aatatcacca
     1261 tgttgttcat agtattcaat tctttcttct aattctgtaa agatgtctgc aaaaatagcc
     1321 caccatcttc gatctacata atcatttaat tggataccat tctcaccata catttctaac
     1381 cgatccgagc ctttaacaat gcttttaaaa atgtttgatt caccatactt atcaattgtt
     1441 ttttgatagt ttgcgtgttg agaggaacgt cgcactgaga aaagaacagg caaaattcca
     1501 atgatttgaa gatgagactc ttcaaaattc gtgacaatcg gaactaaaac tttattaata
     1561 aaatcttctg ttccttccat tgcgtaccgt tttaattctt gcattgcaat aatataatct
     1621 gcagcagtta agaatgcacg aaccacatta tctgttgatg gtgggcagtc gataaagata
     1681 taatcgaaat tttttctcaa ttcttctaaa ggtttaatga aggccaatga tctcttacga
     1741 cgatcaggat atttttcgga caagtattca ttaagttctt cagatccagg agagccagcc
     1801 ataatatata aatgtggact tacttgagta atacctgaaa cccagtcccc ttttttgatt
     1861 gctgcagaca tggatcgatc aaagctagta atttgatacg ttgatccaat gttggaagta
     1921 gtatttcttt gttggtcata atcaattagc aacacgtctt tttcaaaaag ttgactagaa
     1981 acaatcgcta ataaatcatt gtctgtagtc ttaccaatgc cgccttttct acctgtaagc
     2041 attacaactt ttcctagttt atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt
     2101 ccctccaatt tcctatacta aatatattta gtatacttca tatacttaat atattcgata
     2161 tatctaatat actccaaatt gataagaatt cagactttat aaggaatata gtggatatac
     2221 taaatatatt tgctatattc agtatatcag atatattcaa aatgtcaaaa tatatttgtg
     2281 cgttttgcgt tgacaattta gagtgaaaag aggtaaactt aattctatag aaaccacaga
     2341 taattcacta tttacaaagt agcaaaagca gtggtactct ttaaacagaa aaattacata
     2401 aaaaaaagag acggaaataa ttgattttag cagaatcaat tatttctgaa tcacatggtt
     2461 aggacatgtg ttcacgctcg tctcaagaat atgtaagtag ctaccaacta cttatattaa
     2521 aagttgatcg gatacctttt ccgattgcct ttattctacc aaaaaaaggc gagcaactca
     2581 acagaagaac gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac
     2641 acatactatg gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt
     2701 acaatcgatg ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa
     2761 atatcgaaac agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat
     2821 ctttgcgtac cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga
     2881 agaagctatt ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt
     2941 tggtacttat gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag
     3001 tcaaatcaac acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat
     3061 tttagcctgt ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg
     3121 ttatcaagtt tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa
     3181 ggtgatcgat gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt
     3241 gtccggagtg gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa
     3301 tattgttttt aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat
     3361 gaagcttgaa tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc
     3421 tgagaaaaaa gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc
     3481 aaacatcgtc ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt
     3541 agcttattac tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa
     3601 cgaacggtta caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta
     3661 ttctggtaat tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt
     3721 ttcaccaacc ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa
     3781 aaagccacga gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc
     3841 ttatctaagc gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt
     3901 ggcttcacaa ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga
     3961 ggagaataaa gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc
     4021 agtcaaaacg ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc
     4081 aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca
     4141 attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg
     4201 aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg taggtgttgc
     4261 tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat
     4321 catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt
     4381 atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag
     4441 gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt
     4501 cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt
     4561 gtgttagagc aggagtatat tgagtataaa atagctaacg agttaagaa
//
LOCUS       DAFCNJ010000116         4475 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000116 DAFCNJ010000000
VERSION     DAFCNJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.122"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            323..1054
                     /locus_tag="LNE35_002800"
     CDS             323..1054
                     /locus_tag="LNE35_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK6911883.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="LNE35_002801"
     CDS             1199..3226
                     /locus_tag="LNE35_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBK6911884.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="LNE35_002802"
     CDS             3334..4431
                     /locus_tag="LNE35_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK6911885.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DAFCNJ010000117         4433 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000117 DAFCNJ010000000
VERSION     DAFCNJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.123"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(543..2048)
                     /locus_tag="LNE35_002803"
     CDS             complement(543..2048)
                     /locus_tag="LNE35_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6911886.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="LNE35_002804"
     CDS             complement(2045..2731)
                     /locus_tag="LNE35_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6911887.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LNE35_002805"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LNE35_002805"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBK6911888.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAFCNJ010000118         4416 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000118 DAFCNJ010000000
VERSION     DAFCNJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.124"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..913
                     /locus_tag="LNE35_002806"
     CDS             59..913
                     /locus_tag="LNE35_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK6911889.1"
                     /translation="MENSLYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="LNE35_002807"
     CDS             1010..3259
                     /locus_tag="LNE35_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK6911890.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="LNE35_002808"
     CDS             3262..>4416
                     /locus_tag="LNE35_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6911891.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          626 c          805 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct ctatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acaggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DAFCNJ010000119         4345 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000119 DAFCNJ010000000
VERSION     DAFCNJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.125"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(387..1853)
                     /locus_tag="LNE35_002809"
     CDS             complement(387..1853)
                     /locus_tag="LNE35_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6911892.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="LNE35_002810"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="LNE35_002810"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK6911893.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DAFCNJ010000120         4324 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000120 DAFCNJ010000000
VERSION     DAFCNJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.126"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..281)
                     /locus_tag="LNE35_002811"
     CDS             complement(<1..281)
                     /locus_tag="LNE35_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK6911894.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="LNE35_002812"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="LNE35_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="LNE35_002813"
     CDS             complement(2359..2721)
                     /locus_tag="LNE35_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911895.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="LNE35_002814"
     CDS             complement(2725..3063)
                     /locus_tag="LNE35_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911896.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="LNE35_002815"
     CDS             complement(3042..3461)
                     /locus_tag="LNE35_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911897.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="LNE35_002816"
     CDS             complement(3454..3687)
                     /locus_tag="LNE35_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911898.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="LNE35_002817"
     CDS             complement(3684..>4324)
                     /locus_tag="LNE35_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6911899.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DAFCNJ010000121         4282 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000121 DAFCNJ010000000
VERSION     DAFCNJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.127"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..1257)
                     /locus_tag="LNE35_002818"
     CDS             complement(37..1257)
                     /locus_tag="LNE35_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBK6911900.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="LNE35_002819"
     CDS             complement(1277..1888)
                     /locus_tag="LNE35_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK6911901.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="LNE35_002820"
     CDS             complement(1931..2863)
                     /locus_tag="LNE35_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBK6911902.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="LNE35_002821"
     CDS             complement(3284..4144)
                     /locus_tag="LNE35_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6911903.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAFCNJ010000122         4153 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000122 DAFCNJ010000000
VERSION     DAFCNJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.128"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..106
                     /locus_tag="LNE35_002822"
     CDS             <1..106
                     /locus_tag="LNE35_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6911904.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="LNE35_002823"
     CDS             complement(359..508)
                     /locus_tag="LNE35_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911905.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="LNE35_002824"
     CDS             970..1182
                     /locus_tag="LNE35_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911906.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="LNE35_002825"
     CDS             complement(1460..3259)
                     /locus_tag="LNE35_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK6911907.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="LNE35_002826"
     CDS             complement(3383..3979)
                     /locus_tag="LNE35_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK6911908.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DAFCNJ010000123         3923 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000123 DAFCNJ010000000
VERSION     DAFCNJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.129"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(573..1130)
                     /locus_tag="LNE35_002827"
     CDS             complement(573..1130)
                     /locus_tag="LNE35_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911909.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="LNE35_002828"
     CDS             complement(1309..1920)
                     /locus_tag="LNE35_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911910.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="LNE35_002829"
     CDS             complement(2033..2290)
                     /locus_tag="LNE35_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911911.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="LNE35_002830"
     CDS             complement(2887..3165)
                     /locus_tag="LNE35_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911912.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="LNE35_002831"
     CDS             complement(3199..3450)
                     /locus_tag="LNE35_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911913.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DAFCNJ010000124         3875 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000124 DAFCNJ010000000
VERSION     DAFCNJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.130"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            158..2257
                     /locus_tag="LNE35_002832"
     CDS             158..2257
                     /locus_tag="LNE35_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK6911914.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="LNE35_002833"
     CDS             2453..3316
                     /locus_tag="LNE35_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK6911915.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3875
                     /locus_tag="LNE35_002834"
     CDS             3421..>3875
                     /locus_tag="LNE35_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6911916.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1543 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaa
//
LOCUS       DAFCNJ010000125         3770 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000125 DAFCNJ010000000
VERSION     DAFCNJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.131"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            55..1023
                     /locus_tag="LNE35_002835"
     CDS             55..1023
                     /locus_tag="LNE35_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6911917.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1034..1849
                     /locus_tag="LNE35_002836"
     CDS             1034..1849
                     /locus_tag="LNE35_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBK6911918.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            1917..2627
                     /locus_tag="LNE35_002837"
     CDS             1917..2627
                     /locus_tag="LNE35_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6911919.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2663..>3770
                     /locus_tag="LNE35_002838"
     CDS             2663..>3770
                     /locus_tag="LNE35_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBK6911920.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAY"
BASE COUNT         1246 a          554 c          846 g         1124 t
ORIGIN      
        1 tacttttatt ttggataaaa ttaacatttt taaaatttta aggaggaaat gttgatggat
       61 gtagcaaaaa cggttaacgc aattgtagaa aaaaatgatg gagagatttc tggaatttat
      121 ttggtggctt gtggcggttc gctggtagat atgtatgtct cagattattt tcttaaagcc
      181 gaagcatcga agtgtattac aggcttatat acagcgaacg aatttaacca tgtaacccca
      241 aagcaattag atgataaatc tgttgtcatt ctatgttctc atggaggaaa tacagcagaa
      301 acggtaaaag caggagaagt cgctcaagct agaggagctg tgactgttgg tctgactcat
      361 aataaaactg cggatttatt aaaggtttct gatcatagtt tcctttacga atggggagat
      421 gatgcaaaaa tagtcaataa tcctatggcc atcattcttg atttaactgt atctttggtc
      481 aatgttaccg aaacatttga acattacggt aaattcagag aaggcatgaa ggttatcgat
      541 accgtaattg aaaatggcat taagcaggtg caggatcgta ttaaagtgtt tgctgataag
      601 tacaaagatg aaacaatgtt ttatatctta ggtagtggac catcatatgg acatgcttat
      661 ggtttttcaa tttgctcttt gatggaaatg caatggcttg attcaacttc tgttcattct
      721 ggagaattct tccatggacc ttttgaagta acagacaaag atacgaattt tattctcttg
      781 atgagcattg gacgcactcg tgctttagat gaacgggcaa aacgattcct tgataaatat
      841 gcagaaagag tagaaatcgt tgatgcaaag gaactaggat tggatatgat atcagatgaa
      901 gtttctgaat tcttcaatcc cattttattc tacagtattc tttcagaata tcgttcagct
      961 ttagcagatg tacgcaatca tgatttagat gttcgtcgct atatggggaa agtggagtac
     1021 taggaggatt aaaatgaagg tattaggctt aggtgataac gtcgtcgata aatatgaaaa
     1081 tctgggaatt atgtatcctg gaggaaattc attgaatttt gcagtttttg caaaaaaact
     1141 gggacatgaa agtagcttta tgggagtttt tggctcagat gaggaggcag ctcatgtttt
     1201 atcagcaata aaagaaatag gactggacaa cagtcactct cgctttgaaa aaggcgagaa
     1261 tggctgtgcc agagtgaaga ttgatgaggg cgaccggatt ttccttggca gcaattcggg
     1321 aggtgttact cgtgaatacc cgattcagct tactgaagaa gacagagaat atttaaacca
     1381 gtttgaattg attcatatgg gattatatag ccatgtcaat catctattag aagagttaca
     1441 aaatgtttct ggaaagattt cttatgattt ttctgacgac tttacggaag aagaggttca
     1501 gcgggcgatt gataaagttg attttggctt cttttctata gaagactttt ccgatgaaga
     1561 agtaaagcag tttttgatga agcatttttg tgagcgaaat gatgtattga ttgcaacccg
     1621 aggagaaaaa acggctattg gttatgatgg agcaaagttt tatgaagtga accccgtgct
     1681 agaagaacca gttgatacaa tggcagcggg agattctttc ttaacagctt tcctacttta
     1741 ttttctcgaa ggggacgata tagtcaccgc gatgaaaaag gggaatgaat ttgctgggaa
     1801 gtcttgtctg gttgacggtt cgttcggctt tggtattcat tacgattgag cttgttttaa
     1861 attcctgcat ttgataacct aaagaaagaa atcccggtaa agtggaggtt aaaaaaatgc
     1921 tggatacaaa tgcacaatta ccattgtact tacaattaaa agaaatatta aaaaataaaa
     1981 tcaatacagg ggagtatccc gagaatacga aaattcccgc tgaatcagag ctgatagaaa
     2041 gttatgaggt cagccgaatc acagtgagaa aagcagtaga agaactggta gaagaagggt
     2101 acctacttaa aaaacaaggg aaaggaactt ttgtcaaccg gcataaagtt tttcgtaaaa
     2161 ttgaatatat tatgggtttc agtgagagtt gtaaatctaa tggttttaca ccgactagca
     2221 ttgttttgga acgcaagatc attccagcaa ctgaagggat ggcaaaaaaa ctcgatatcg
     2281 ctcaaggtga tgatgtttta tatatccaga gaaagcgaat ggcggataac atccccatta
     2341 tgttagaaaa caattatttt tcttatgaaa gatatagttt cttaatggag gaagagctga
     2401 ctgattcttt gtatgaagta cttaaagtcc aaggtacctt agccatcaat cctggggaaa
     2461 ctgcacttga aattgtcaca gctgatgacc aaattgccaa aataatggaa gtagctgttg
     2521 ggacgccctt tttctttatg gatactttaa tcaacgatca aaacaacata ccaattcatg
     2581 taggtaatca atattacctg ggagaagtat atcgttttta cttatgattt cctgtttgga
     2641 tttcaaggag tagattttat ttatgaagca aatattgcgg tcaaaaatga tttttgatgg
     2701 agagcagcca cctttcaaag gttatattgt aatcaatgaa gggatcattg aaaaggtgga
     2761 aaacaactgg aattacagtg atctattaaa tgaaaacacg gaattaattg cttatgatga
     2821 tcaattcgtt atgccagcaa ttcatgataa tcatgtattt ttcagtggtt atttggctat
     2881 gaatgctggc atagatttaa gcgatacatc ttcagctaac gaggctgtag agcgtattaa
     2941 agctgtaatg aaccataaaa aagcagatga accaatttat gctcatggct gggatgctgc
     3001 cctgtggaaa actgacccat cagaagagct attgaatgaa ataaaccact ttggtccaat
     3061 cacagcgatc gacaaaaatc gcagccattg ttggatgaat gctattgcta agcagactta
     3121 tggttttaca gaaaaagaaa ccagtgctga aagtcgagta ttattaatta aggaaatgct
     3181 agcaaacaaa aagcttttag cagaggttta cgctgaattt gaagcattac tgctttcaaa
     3241 aggtgttata tcaatgaaag aaatcgtttt tgatgacgca gaatttcttt ctaatatacc
     3301 tcagaggaaa atacttagta acctatatgt gcaggcagtc caagaacctc tacagcatga
     3361 acttctatat gagtataagg aaaaagtttt ccctgaaaag gtccgctttg gtggagtgaa
     3421 gatcatggta gatggtgttg ttgcggatag aagtggagat atttacggtg tttatcagtc
     3481 aggagtagct tcacccgaga ttgactatga ggcaattaaa gcagaagttg ctgcattcaa
     3541 tgaagtagag attccttgct gtttgacaac ggaaggagat aaagcgggtt ataaagctgc
     3601 gtcaattctt gtcgaatttg gaaagcggtt acctgaaggc gtttttaatt cgattagtga
     3661 tttggaaatg gttactcaaa agacagcaga aaaaatgaac gaaggacaag tagtagcaga
     3721 ggtatatcca caaattctcg gcttgaatca aacgttagaa gaggcttaca
//
LOCUS       DAFCNJ010000126         3712 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000126 DAFCNJ010000000
VERSION     DAFCNJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.132"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LNE35_002839"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LNE35_002839"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBK6911921.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="LNE35_002840"
     CDS             complement(2260..2511)
                     /locus_tag="LNE35_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBK6911922.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="LNE35_002841"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="LNE35_002841"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBK6911923.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="LNE35_002842"
     CDS             3461..3679
                     /locus_tag="LNE35_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911924.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DAFCNJ010000127         3262 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000127 DAFCNJ010000000
VERSION     DAFCNJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.133"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            54..734
                     /locus_tag="LNE35_002843"
                     /pseudo
     CDS             54..734
                     /locus_tag="LNE35_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            796..1413
                     /locus_tag="LNE35_002844"
     CDS             796..1413
                     /locus_tag="LNE35_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911925.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            2433..>3262
                     /locus_tag="LNE35_002845"
     CDS             2433..>3262
                     /locus_tag="LNE35_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK6911926.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT         1179 a          463 c          559 g         1061 t
ORIGIN      
        1 caaaaaatct attttagtgt aaaattgaga aaaaagacag agaggacaga gtaatgaatc
       61 attttaaagg caaacaattc aaaaaagacg tcattattgt cgctgttggt tactacctgc
      121 gttacaatct aagctatcgt gaagttcagg aattgttata tgatcgtgga ataaatgttt
      181 gtcatactac gatttatcgt tgggtataag agtacagcaa agtcctctat tatctttgga
      241 agaagaaaaa tagacaatcc ttctattcat ggaaaatgga cgaaacctat atcaaaatta
      301 agggacgttg gcattatctt tatcgtgcaa ttgatgcgga cggcttaacc ttagatatct
      361 ggttacgaaa gaaacgggat acgcaagcag cctatgcttt cttaaaacga ctccataaac
      421 agtttggtga gccgaaagca attgtgaccg ataaagcacc ttctcttggc tccgccttta
      481 gaaagttaca gagtgtgggt ttatatacta agacagagca ccgaactgtg aagtatctta
      541 acaatttaat agaacaagac catcgaccta ttaaacgacg aaataaattt tatcaaagtc
      601 tccgtacagc ctcttccacg attaagggca tggagaccat tcgaggaata tataaaaaga
      661 accgaagaaa tggaacgctc ttcggctttt cggtgtctac tgaaatcaag gtattaatgg
      721 gaataacagc ctaagatatt tggagttcag agagggcgcg tttgattttc aaacttcgca
      781 acagaaccga gaaccttggg ttttcaagtg ctttttggtt cattaatctt aaccatagtg
      841 tttttaaagt ggaaaacaag caacaaaaaa agtatgtgga gccttgtagg aatagttctt
      901 attattttta ctgtgactgc agcaacagaa gcaaattatt cgcataaaat tcagtcgtat
      961 aaaggtcgtt tagttatgac caaaaaagat gttacacgtt atacatcctc aagaactgga
     1021 ggcctatatg actatgcacc attagatgca ttgaaatata aagatcattt aaagaaacat
     1081 gaagtaaaag ttggtgatcg gtgggagaat gcatctcata aagcatataa ttccaaaata
     1141 gtctatacag tagaagatgc cgatgagcaa aatatcacat tacctgtatt caattattgg
     1201 ggaacagttg tgaaagtaaa tgggaaaaag agcactatag aaaatgacaa tggtttagtg
     1261 tctattaaag gtacggggaa aagaactata gttgaggttt catctacgta tccaactacg
     1321 atgattctat ctttattaat tagttcaata acttttatat tcttatttac acgttattta
     1381 ataagatgct gcggtgaaat gaaaatagat tagatagata ttataaatat gttgattcta
     1441 ttttcatagc aatggagaaa atacattagc aatatataaa agaaggtatc aagagtcaca
     1501 gtgaatttat tccgaaaagt gaaaagagca aatataaaat ttaatgaaaa tatagaacta
     1561 aataggaaaa cgtagacgta attttctacg ttttcctttt catatatatt aaatattacg
     1621 gaaaattata taaactaatt tagtgttttg tttgtaattc ctaattttgc tccactttta
     1681 gataattttt aggtctattt aactcatcag acattctatg ctcatttttc tccaaatttg
     1741 tttcaactct ttcgtactta attttgcgta tttgaaaatt cttcttttag agtactttta
     1801 ttacgatact agtatgatat atatctatag caaacagtta aaatcttcct aaaataagcg
     1861 aatagcattc ctaaaaccta taaaattttt agatgaagcg acaagtggac aattaataaa
     1921 ctacttgaac atgaaattat ggatcacctt tcaaatttag aaaatggaat tcttatactt
     1981 attgcgcaac atttattgat tgcaaaatta tgtgacaaaa aaaactagta gaagaaagaa
     2041 taatggcttg ataataaagt actacctatt aaaggtagta ctttatttaa tttagtataa
     2101 attaacatac tactatattt taggtttcat attatataaa ttaataatat actaccattt
     2161 gttggtagta taatttaaag gtaataagtt attcttttat tactatgttt gggttttata
     2221 gcaaagaaag tttaataaga gtttggacct agcaaacaat attaatgagg tcattaaatc
     2281 ttattatctt tagtgatata acgaaatatc tacacagaca aaatgatagc gtaaaatatt
     2341 ttgtgtaaat agatatttag ggtagaaaaa aagaaagtcc attctgtaaa attaaagtta
     2401 ccacaaccga aattttatag gaggactttc tcatgcccaa ttttactaca gaaattatgg
     2461 aaacactaat caataaaggt gatttagatg aattatttcg atgtcattta gaattagctg
     2521 ttaattcatt attgcaggct gaattaacag cttttcttga ttatgaaaaa tatgatcgtg
     2581 ctgggttcaa ttcaggtaat tcccgtaatg ggaattattc acgttcattc agaacagagt
     2641 atggagaatt aaatttggta attcctagag atagaaatgg taagttttct caacaaactt
     2701 tacctgctta taaaagaacc aatgactctt tagaaaccac tattattcag ctatttcaaa
     2761 aaggaattac tatgtctgaa atctctgaat tgattgaaaa aatgtacggg catcactata
     2821 cgccacaaac catttcaaat atgactaaaa tagtttctga agatattatt gcctttaaag
     2881 aaaggtcctt agaatccaga tactctgtca tttttatgga tgctactcac attcctttaa
     2941 aaagacaaac tgtttcaaaa gaagcagttt atattgtaat tggcattcga ttagatggaa
     3001 ccaaagaggt tcttgggttt agtcttgctc caactgaatc tgcttatgtt tggaaagaga
     3061 ttcttcaaga cctaaaagac cgtggtttaa aagaggtttt attagtcgta actgatggtt
     3121 taagtggcat taacgatagt attcatagtg tttatccaaa tgctcaattt caacaatgtt
     3181 gtgtccatat atctagaaat atcgctcata aggttcgtgt tagtgatcga caagaaatct
     3241 gtagcgattt caaattagtt ta
//
LOCUS       DAFCNJ010000128         3221 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000128 DAFCNJ010000000
VERSION     DAFCNJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.134"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            301..585
                     /locus_tag="LNE35_002846"
     CDS             301..585
                     /locus_tag="LNE35_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911927.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="LNE35_002847"
     CDS             588..1004
                     /locus_tag="LNE35_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911928.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="LNE35_002848"
     CDS             complement(1141..1860)
                     /locus_tag="LNE35_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6911929.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="LNE35_002849"
     CDS             2535..2630
                     /locus_tag="LNE35_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK6911930.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="LNE35_002850"
     CDS             complement(2932..3177)
                     /locus_tag="LNE35_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911931.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAFCNJ010000129         3221 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000129 DAFCNJ010000000
VERSION     DAFCNJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.135"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..698
                     /locus_tag="LNE35_002851"
     CDS             <1..698
                     /locus_tag="LNE35_002851"
                     /inference="COORDINATES: protein motif:HMM:NF013429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK6911932.1"
                     /translation="SNCQAVKQLAQKHALDIIAFTPESIIYPYNIAAPETMQWEKSKA
                     YFRNAILATAELGCSLMTINSGYGLMEEPIEESWKRSREMLRYLAEIAETEGVTIALE
                     TLRPEESKIVTKLQEAQRMVHDVAHPSLKLMVDTAAMGVAGEGLQDWFKAFGDDIVHL
                     HFVDGTPEGHLAWGDGNFNLHEFLTILEKYDYQGFLGQEITDSRYLKRPYLADKQVFD
                     KLSEALRKREGNV"
     gene            695..1483
                     /locus_tag="LNE35_002852"
     CDS             695..1483
                     /locus_tag="LNE35_002852"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911933.1"
                     /translation="MKDFVAVGYACMDIYDDLGKKYPTGNGVDVIAHLSKAGYDVALV
                     SAVGDDTYGKEFIEFCEDMEFDTSHVHVEEGDTAQIHLSLIDGDRVHGKVIDGVLANF
                     SLTKEDRAFIKEFKYLHTDYFGRIYGMLGEFQQAGCKIVFDFSSFINDKDFEMVAPYV
                     DIGQISVEGFSQETKKIMQYMHCLGIKICIATAGSQGALAYDGKNFYCQEALDPQEIV
                     NTVGCGDAFLAGFLMGDAASKPIEECLLLGAKSSQKIIQKFEPY"
     gene            1504..2367
                     /locus_tag="LNE35_002853"
     CDS             1504..2367
                     /locus_tag="LNE35_002853"
                     /inference="COORDINATES: protein motif:HMM:NF016777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBK6911934.1"
                     /translation="MTIDTCFHPIYFDMIQTSEAELKKREEAFGVHKQSPYTMDEILS
                     EMDYGKIEKSILHPIALTTTENVSIVTNEEISKIIQQEPNRFIGFASVDPNNPEAETE
                     LEQAFQKGLAGLTFHPGKQQIYPNDPKMKPLYQLCEKYDKPIYFHSGLSWEPRSYTKF
                     CQPLFVEDVLIEHPALRICLAHFSWPWIQEVMMLMIKYSNLYTDTSMLYLNSFDDFFG
                     DFFSNQLTPSTIERNFPRQIMFGSNTPRFRAFKVKRALEKIPFSKEVRENILTNNAYD
                     YVYGRKSRHDL"
     gene            2357..2785
                     /locus_tag="LNE35_002854"
     CDS             2357..2785
                     /locus_tag="LNE35_002854"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBK6911935.1"
                     /translation="MIYKEYLEFFSTEDVQLINITEEVEKVMARSNITSGVAFILSNH
                     TTTGITINEPLECVEKDIKNTLERLIPENSNYIHAHFLPSYGATSNNSPGHLKSSLLG
                     NHAMFSVEDGKLEKGSAQDIYLVECDGPQTRKILIKILGE"
     gene            complement(3084..>3221)
                     /locus_tag="LNE35_002855"
     CDS             complement(3084..>3221)
                     /locus_tag="LNE35_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6911936.1"
                     /translation="REVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSL
                     I"
BASE COUNT         1039 a          545 c          683 g          954 t
ORIGIN      
        1 attctaattg ccaagccgtt aagcaactag cacagaaaca tgctcttgat atcattgctt
       61 ttactccaga atcaattatc tacccttata acattgctgc gccagagacg atgcagtggg
      121 aaaaaagcaa ggcctatttt cgaaatgcaa tattagctac ggctgagtta ggctgctcac
      181 tgatgacaat caactccgga tatggtttga tggaagagcc tatcgaagaa agctggaagc
      241 gctcaagaga gatgctacga tacctcgctg aaatagcaga aactgaggga gtcacgattg
      301 cattggaaac gttgcgtccc gaagagtcga aaattgtaac caaattacag gaggctcagc
      361 gaatggttca tgatgtggca cacccatctt tgaagctgat ggttgatacc gcagcaatgg
      421 gggtcgctgg agaaggtctt caagattggt ttaaagcctt cggggatgat attgtccacc
      481 ttcattttgt tgatggaaca ccagaaggac atcttgcctg gggagacgga aattttaatt
      541 tacatgagtt tcttactatt ttagaaaaat atgattatca gggatttctg ggacaagaaa
      601 tcacagatag ccgctatcta aaacggcctt atcttgcaga caaacaagta tttgataaat
      661 tatcagaagc attgaggaaa agagagggaa atgtatgaag gattttgttg ctgtaggcta
      721 tgcatgtatg gatatctatg acgatttagg gaagaaatac cccactggaa atggagtgga
      781 tgttatagct catctctcaa aagctggtta cgatgtagct cttgtttcag cggtaggaga
      841 cgacacttat ggtaaagaat tcattgaatt ttgcgaggat atggaatttg atacttctca
      901 tgttcatgta gaggagggag atacagcaca aatacacttg tctctgatag atggggatcg
      961 agttcatgga aaagtaattg atggtgtact agcaaatttt tccttaacaa aagaggaccg
     1021 ggcattcatc aaagagttca agtatctaca cacagattat tttggccgca tctatgggat
     1081 gctaggagag tttcaacaag ccggctgtaa aattgtattt gatttttctt cttttatcaa
     1141 tgataaggat tttgaaatgg ttgctcccta tgttgatatt ggacagattt ctgtagaagg
     1201 attctctcag gaaactaaaa aaattatgca gtatatgcat tgtttaggaa tcaaaatctg
     1261 cattgccact gctggaagtc aaggggctct cgcctacgat ggaaagaatt tttattgtca
     1321 agaagcgctt gatcctcaag aaattgtcaa tacagtgggg tgtggggatg ctttcctcgc
     1381 gggtttccta atgggagatg cagctagcaa acctattgaa gaatgtttgt tattgggagc
     1441 taaaagttca caaaagatca ttcagaaatt tgaaccttat taattgacag aggagcggaa
     1501 gtaatgacca tagatacttg ttttcatcct atctatttcg atatgattca aacatcagaa
     1561 gcagaattaa aaaaaagaga agaggctttc ggtgtacaca aacaaagccc ctatacaatg
     1621 gacgaaattc tttcggagat ggattatgga aagatagaaa aatcaattct tcatccaatt
     1681 gctctaacga ctaccgaaaa tgtcagcatt gtgacaaatg aggagattag caaaattatc
     1741 caacaggagc caaatcgttt tattggtttt gccagtgtag atcccaataa tcccgaggca
     1801 gagaccgaac tggaacaagc ctttcaaaag ggacttgccg gtttaacttt ccatcctggg
     1861 aaacagcaaa tttatccgaa tgatcctaaa atgaaaccgc tttaccagtt atgtgaaaaa
     1921 tatgacaagc caatttattt tcactctggt cttagctggg agccaagaag ctacacaaag
     1981 ttctgtcagc cactatttgt ggaagatgta ttgattgaac atcctgcgct tcgaatctgt
     2041 ttagcacatt tttcatggcc ctggattcaa gaagtgatga tgttgatgat aaaatattca
     2101 aatttgtata ctgatacctc tatgctttat ttgaactctt ttgatgactt ctttggagat
     2161 ttttttagca atcaattgac tccctcaaca attgagcgaa attttccaag gcaaattatg
     2221 tttggatcaa atacaccacg ttttcgagcc ttcaaagtga aacgggctct ggaaaagatt
     2281 cccttctcaa aagaagtgag agaaaacatc ttgacaaata acgcttatga ttatgtatat
     2341 gggaggaaaa gtaggcatga tctataaaga atacttagaa ttcttttcca cagaggatgt
     2401 ccagttaata aatattactg aagaggtaga aaaggttatg gcgcggagca atataacaag
     2461 tggcgttgcc tttatcctta gtaaccatac aacaacgggc atcacaatca atgagccact
     2521 ggaatgcgtg gagaaggaca taaagaatac actagaacga ttaatccctg agaattcgaa
     2581 ctatattcat gcacattttc ttccctcata tggagcgaca agtaataact ctccaggaca
     2641 tttaaaatcc tccttactag gaaatcatgc aatgttttca gtggaggatg gtaagctgga
     2701 gaaggggagt gcccaagata tttatttagt agaatgtgat ggacctcaaa ctcgcaaaat
     2761 tctaataaaa atactaggag agtagttttg aattaccgga agggactggg acaaaagttt
     2821 tttcttaaaa aatagtccca tttcttcagt attaattaaa taaaaagtaa aatacattcc
     2881 cgaaactatt ggatgaagat cccagtagtt tcgggaatgt attttttcta gaggctttct
     2941 tttgctcttt gtcaagaagt gttgatgacg agattttcta attcaaatga aggataattt
     3001 cacaatctat taagcagtgg ctaggaacca attggttcct aagttgctgc ttttatttct
     3061 aataatttat attctatgat ttgtcaaatt aagctagaat aaaatgcttc ttgtacatag
     3121 ctcaaaaata tttcgattgg tgttctgtaa ttcaatgatt ttcttggaat atgattacgt
     3181 tgattgctga cactggaaat aaatgtctga ttcacttctc t
//
LOCUS       DAFCNJ010000130         3012 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000130 DAFCNJ010000000
VERSION     DAFCNJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.136"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            177..359
                     /locus_tag="LNE35_002856"
     CDS             177..359
                     /locus_tag="LNE35_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBK6911937.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="LNE35_002857"
     CDS             462..746
                     /locus_tag="LNE35_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911938.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="LNE35_002858"
     CDS             762..998
                     /locus_tag="LNE35_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911939.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="LNE35_002859"
     CDS             1020..1274
                     /locus_tag="LNE35_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911940.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="LNE35_002860"
     CDS             1356..1541
                     /locus_tag="LNE35_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBK6911941.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="LNE35_002861"
     CDS             1608..2003
                     /locus_tag="LNE35_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK6911942.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="LNE35_002862"
     CDS             1993..2370
                     /locus_tag="LNE35_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911943.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="LNE35_002863"
     CDS             2392..2766
                     /locus_tag="LNE35_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911944.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DAFCNJ010000131         3010 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000131 DAFCNJ010000000
VERSION     DAFCNJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.137"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1412
                     /locus_tag="LNE35_002864"
     CDS             <1..1412
                     /locus_tag="LNE35_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6911945.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="LNE35_002865"
     CDS             1546..2889
                     /locus_tag="LNE35_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6911946.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1197 a          406 c          578 g          829 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa
     2941 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
     3001 atgaaaaaat
//
LOCUS       DAFCNJ010000132         2980 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000132 DAFCNJ010000000
VERSION     DAFCNJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.138"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            8..649
                     /locus_tag="LNE35_002866"
     CDS             8..649
                     /locus_tag="LNE35_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911947.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="LNE35_002867"
     CDS             708..1148
                     /locus_tag="LNE35_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911948.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="LNE35_002868"
     CDS             1160..1828
                     /locus_tag="LNE35_002868"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK6911949.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="LNE35_002869"
     CDS             1892..2515
                     /locus_tag="LNE35_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBK6911950.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="LNE35_002870"
     CDS             2571..2957
                     /locus_tag="LNE35_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBK6911951.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
BASE COUNT         1065 a          491 c          619 g          805 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata
//
LOCUS       DAFCNJ010000133         2914 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000133 DAFCNJ010000000
VERSION     DAFCNJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.139"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1364)
                     /locus_tag="LNE35_002871"
     CDS             complement(45..1364)
                     /locus_tag="LNE35_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6911952.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="LNE35_002872"
     CDS             complement(1361..2014)
                     /locus_tag="LNE35_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6911953.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..>2914
                     /locus_tag="LNE35_002873"
     CDS             2302..>2914
                     /locus_tag="LNE35_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6911954.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          955 a          474 c          321 g         1164 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tattgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc
     2701 tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca gccacgtatg
     2761 aacttccttc cgacggtgtt gtgtatggat gagttcaaat caatgaagtc tgtttctggt
     2821 tccatgagtt ttatttgtgt tgatgggaca acccatcagc tatttacgat ccttgaagat
     2881 cgcagacttt acaaactaac tcaatacttc ctgc
//
LOCUS       DAFCNJ010000134         2886 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000134 DAFCNJ010000000
VERSION     DAFCNJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.140"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            798..1052
                     /locus_tag="LNE35_002874"
                     /pseudo
     CDS             798..1052
                     /locus_tag="LNE35_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="LNE35_002875"
     CDS             1122..1634
                     /locus_tag="LNE35_002875"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6911955.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="LNE35_002876"
     CDS             1631..2668
                     /locus_tag="LNE35_002876"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6911956.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1041 a          513 c          574 g          758 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
     2881 ctctac
//
LOCUS       DAFCNJ010000135         2739 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000135 DAFCNJ010000000
VERSION     DAFCNJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.141"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="LNE35_002877"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="LNE35_002877"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK6911957.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DAFCNJ010000136         2514 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000136 DAFCNJ010000000
VERSION     DAFCNJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.142"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(543..1883)
                     /gene="glnA"
                     /locus_tag="LNE35_002878"
     CDS             complement(543..1883)
                     /gene="glnA"
                     /locus_tag="LNE35_002878"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBK6911958.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1911..2303)
                     /locus_tag="LNE35_002879"
     CDS             complement(1911..2303)
                     /locus_tag="LNE35_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6911959.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          728 a          551 c          424 g          811 t
ORIGIN      
        1 aggagatgcg acaaaagtcg tgcctcctta tttctaaata aacggtgttc tcctgccaac
       61 tcctcggtca caagtcacaa tatccatcaa acatgagaag ctgtttttga aattatttct
      121 tgtgattcgg agttaaatgg taggctcaca acctctggat aaacggtatt caaaagccag
      181 tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt
      241 cttaatactc gaaactaaac gtctttttca aaacctcttg gtaaacggtg ttcaaaagct
      301 tgcatctgcg gtaataagtt cagccaattc aaaaacaaga aaagctgttt ttgcttggct
      361 attcttatta cttgttgcag ggcagctttt tcacaacctc tccactaaac ggtgttcaat
      421 cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat ttttccgtta
      481 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
      541 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      601 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      661 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      721 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      781 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      841 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      901 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      961 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
     1021 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
     1081 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
     1141 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
     1201 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
     1261 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
     1321 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
     1381 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
     1441 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1501 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1561 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1621 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1681 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1741 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1801 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1861 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1921 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1981 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     2041 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     2101 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     2161 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     2221 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     2281 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     2341 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     2401 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     2461 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DAFCNJ010000137         2488 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000137 DAFCNJ010000000
VERSION     DAFCNJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.143"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            191..445
                     /locus_tag="LNE35_002880"
     CDS             191..445
                     /locus_tag="LNE35_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6911960.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="LNE35_002881"
     CDS             446..763
                     /locus_tag="LNE35_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6911961.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="LNE35_002882"
     CDS             1322..2257
                     /locus_tag="LNE35_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911962.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILITRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            2351..>2488
                     /locus_tag="LNE35_002883"
     CDS             2351..>2488
                     /locus_tag="LNE35_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK6911963.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          921 a          342 c          457 g          768 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga aacgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttta ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataactc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag gaaaatgtct
     2281 aaacatgtta gtaaatcaac aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg
     2341 aggactacat atgcgttcat caaaatatac tgaacattat acagatactt ttataacatt
     2401 caaggaaatt atgagaactg tttctaacat ataccacaat tgtgtaccag ataagattaa
     2461 aaaccgaaga aatactgatc aactgaag
//
LOCUS       DAFCNJ010000138         2412 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000138 DAFCNJ010000000
VERSION     DAFCNJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.144"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..515
                     /locus_tag="LNE35_002884"
     CDS             123..515
                     /locus_tag="LNE35_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911964.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="LNE35_002885"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="LNE35_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            857..1054
                     /locus_tag="LNE35_002886"
     CDS             857..1054
                     /locus_tag="LNE35_002886"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911965.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="LNE35_002887"
     CDS             complement(1097..1672)
                     /locus_tag="LNE35_002887"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBK6911966.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="LNE35_002888"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="LNE35_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          765 a          413 c          377 g          857 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tc
//
LOCUS       DAFCNJ010000139         2408 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000139 DAFCNJ010000000
VERSION     DAFCNJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.145"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(243..464)
                     /locus_tag="LNE35_002889"
     CDS             complement(243..464)
                     /locus_tag="LNE35_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911967.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="LNE35_002890"
     CDS             complement(562..807)
                     /locus_tag="LNE35_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911968.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="LNE35_002891"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="LNE35_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2408)
                     /locus_tag="LNE35_002892"
     CDS             complement(2031..>2408)
                     /locus_tag="LNE35_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HBK6911969.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          817 a          419 c          331 g          841 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctac tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattgg
//
LOCUS       DAFCNJ010000140         2407 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000140 DAFCNJ010000000
VERSION     DAFCNJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.146"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1150
                     /locus_tag="LNE35_002893"
     CDS             5..1150
                     /locus_tag="LNE35_002893"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBK6911970.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="LNE35_002894"
     CDS             1321..1779
                     /locus_tag="LNE35_002894"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBK6911971.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="LNE35_002895"
     CDS             1938..>2407
                     /locus_tag="LNE35_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBK6911972.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAFCNJ010000141         2398 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000141 DAFCNJ010000000
VERSION     DAFCNJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.147"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..2069
                     /locus_tag="LNE35_002896"
     CDS             207..2069
                     /locus_tag="LNE35_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6911973.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="LNE35_002897"
     CDS             2069..>2398
                     /locus_tag="LNE35_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6911974.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAFCNJ010000142         2232 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000142 DAFCNJ010000000
VERSION     DAFCNJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.148"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..543
                     /locus_tag="LNE35_002898"
     CDS             <1..543
                     /locus_tag="LNE35_002898"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6911975.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(899..1471)
                     /locus_tag="LNE35_002899"
     CDS             complement(899..1471)
                     /locus_tag="LNE35_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6911976.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1487..2092)
                     /locus_tag="LNE35_002900"
     CDS             complement(1487..2092)
                     /locus_tag="LNE35_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBK6911977.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          453 c          338 g          740 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
      601 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
      661 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
      721 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
      781 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
      841 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
      901 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
      961 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1021 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1081 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1141 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     1201 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     1261 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     1321 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     1381 tcttctctac gcagttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     1441 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     1501 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     1561 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     1621 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     1681 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     1741 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     1801 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     1861 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     1921 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     1981 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2041 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2101 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     2161 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     2221 ttgcacagtc ta
//
LOCUS       DAFCNJ010000143         2065 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000143 DAFCNJ010000000
VERSION     DAFCNJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.149"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1008
                     /locus_tag="LNE35_002901"
     CDS             <1..1008
                     /locus_tag="LNE35_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911978.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="LNE35_002902"
     CDS             1025..1174
                     /locus_tag="LNE35_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911979.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="LNE35_002903"
     CDS             1171..1617
                     /locus_tag="LNE35_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911980.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="LNE35_002904"
     CDS             1619..1756
                     /locus_tag="LNE35_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK6911981.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="LNE35_002905"
     CDS             1794..>2065
                     /locus_tag="LNE35_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911982.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAFCNJ010000144         1991 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000144 DAFCNJ010000000
VERSION     DAFCNJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.150"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="LNE35_002906"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="LNE35_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK6911983.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="LNE35_002907"
     CDS             852..1040
                     /locus_tag="LNE35_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK6911984.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="LNE35_002908"
     CDS             1053..1610
                     /locus_tag="LNE35_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6911985.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="LNE35_002909"
     CDS             1642..1884
                     /locus_tag="LNE35_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6911986.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          553 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc c
//
LOCUS       DAFCNJ010000145         1962 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000145 DAFCNJ010000000
VERSION     DAFCNJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.151"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..272)
                     /locus_tag="LNE35_002910"
     CDS             complement(<1..272)
                     /locus_tag="LNE35_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911987.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="LNE35_002911"
     CDS             complement(309..446)
                     /locus_tag="LNE35_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK6911988.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="LNE35_002912"
     CDS             complement(448..894)
                     /locus_tag="LNE35_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911989.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="LNE35_002913"
     CDS             complement(891..1040)
                     /locus_tag="LNE35_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911990.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..1386)
                     /locus_tag="LNE35_002914"
                     /pseudo
     CDS             complement(1054..1386)
                     /locus_tag="LNE35_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
BASE COUNT          509 a          407 c          342 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DAFCNJ010000146         1954 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000146 DAFCNJ010000000
VERSION     DAFCNJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.152"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..626)
                     /locus_tag="LNE35_002915"
                     /pseudo
     CDS             complement(1..626)
                     /locus_tag="LNE35_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            647..1813
                     /locus_tag="LNE35_002916"
                     /pseudo
     CDS             647..1813
                     /locus_tag="LNE35_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
BASE COUNT          672 a          304 c          374 g          604 t
ORIGIN      
        1 tttgaaatac ccgattggta gcgatggaag atcgtgaatt tctttttaaa cgactgaggt
       61 aacgtagatg aaattgtttt aagggtttct tgaaagccta ccgtatctcg tttaacgaaa
      121 taatagcgta ataattgaca aaaatgatac gcagttcgca attcttcgtt gtagcttaac
      181 agctcattaa cgatttctgt ttgactaatt ggtcgtttaa acagtcgatg ataatgccgt
      241 ttgagtgcct cccatgtcgt ggaatctttg agtaagagtt tccaatatcg cttgagccga
      301 cggtacttct tctgattttc agagtgtgag gttcgaaaac gattcatgat ctggacacgt
      361 aattgattga agctgcgatt gatgtgttgg acaatatgaa agcggtcagt tacgatctcc
      421 gcatttggaa agaccgtttt aagcaactga caatagctgg cattcatgtc catcactagg
      481 tacttgacct ttaaacgggc ctttctagga aagcgcagga agtattgagt tagtttgtaa
      541 agtctgcgat cttcaagggt cgtaaatagc tgattggttg tcccatcaac acaaataaaa
      601 ctcatggaac cagaagtggc aacattgtgt acttttcttt gcaatcttgt gtacaaaact
      661 attgcaaaac acagcgcata agaatcctta caaaacagta gatgaagcaa cccaattaga
      721 tcttgttata gttgttaatc agctcttaac gggttacgat tcaaaatggg tcaacaccct
      781 atacttagat aagtcacttg aatatcagaa cataattcag gctttttcac gcacaaatcg
      841 tcttaatggt cctgaaaaac cgttcggaac cattcgttat tatcgtaaag cacatacgat
      901 ggaacagaac attgaagagg ctattgagtt atactcaggc aaacaagcaa aagggttatt
      961 tgttgaaaaa ttatataata acttagaaaa gttgaacgat atattcgaag aaattaagtc
     1021 cgtatttaat atttctggca tagaaaactt cagtcattta ccggaggttc caggggaaat
     1081 taagcaattt gtaaatcttt ataatcaact aatccattgt tatgaatcag cgatgataca
     1141 gggattcaca tgggcaaaat cgacttatga aatccaggat tatgaaccta taacagtagt
     1201 aatcacagaa tatgaattta atatcttggc acaacgttac agcgagatag agagaaccta
     1261 gagacggaaa tgatagtata gcgtatgaca ttgattacca attacctgaa ggtagtcgta
     1321 gtcgtattaa ttatgagtat ttaaacacaa acttcaaaaa atacatgaat ataaaagaag
     1381 aacgtgattc gaatttaatt caatcattgt taaacgactt acatcgcaca tttgcgcaat
     1441 taccagtgga gttacagcca tatgctgaga tgattctaaa tgatgtaaat aatggggaat
     1501 tagttatcaa agaaggatgg gaatttactg attacctcaa tgaattttag tggattcaac
     1561 caatattaat gaggcattat tacgtgagat gttggattta cgcttaaccg aggtaaacat
     1621 taatgaatat agtcgatttg ataaattaaa atcaagtgtt aacgtaggtc atttcagtgg
     1681 atatatcgag aaaaatttag gtaaaatggt aataccaatc aaggtaaata tgctaattga
     1741 cagattattg cgtgcatttt tattagaaga tgtattcgat gttacagagt acataaagaa
     1801 ttactttaac tagagtacac ctacatctta tagtacataa aaaaattcat cttttttagt
     1861 ttaataattc accaaaaagg tgagtaatgc catttttgaa aattgagctt gttttatgtc
     1921 tacatctgta tccgtatata atccaaagga ttat
//
LOCUS       DAFCNJ010000147         1875 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000147 DAFCNJ010000000
VERSION     DAFCNJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.153"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(149..619)
                     /locus_tag="LNE35_002917"
     CDS             complement(149..619)
                     /locus_tag="LNE35_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911991.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            706..1404
                     /locus_tag="LNE35_002918"
     CDS             706..1404
                     /locus_tag="LNE35_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBK6911992.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1582..>1875
                     /locus_tag="LNE35_002919"
     CDS             1582..>1875
                     /locus_tag="LNE35_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911993.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYG"
BASE COUNT          685 a          267 c          374 g          549 t
ORIGIN      
        1 gcaagcgttg ataggaacga ttgtttacat ggtttggatt tttatggggc ttgatgaagc
       61 tgaagctgag tacagagcga aaaagccaac cgagggggct gactaaaaat gaatagaaaa
      121 gagaaactag aatgtatatt actattactc agtttaattc tttcactaat ttctctattg
      181 ggaagtttgt atttttgata tcaacaattt tgtggatttg ggttctattt agagtaaata
      241 tcatttttgt ttggtgatta aggagtttat taaaattaat tttccctact tcaaatgcaa
      301 ggtaacaaga tatagcactt ttaggaggaa tatttagcgg tacagcttca gagtatattc
      361 tattatcatc gactgatcct cctttagcaa ttaatttctt aaatggagta gctgaccatc
      421 tgcggtttaa gttagcggat gaaagttcta aattaactaa catagctggt tctgttgaat
      481 agttagaaat gattactttt gtataaatca tatctgcagc gaagtaagaa gcattaagtt
      541 caacttctag ttgagggcgc attcctttta tttttatccc tgttaaaaac gtactcaata
      601 taaaacttat tattgacatc cattgaaaaa ttgttaagtg aaaaaaattc aaaataaaca
      661 ccaccagttt ttaactaaat tataccaaaa aggagagaag aaataatgca agaattagta
      721 attttgaaaa ataaagaagc tgtgactacg agcttacaag tggcagaaag cttcgagaaa
      781 aaacatcaac atgttttaag agatattgat gcactaaaaa aagatgtgtc caattttgga
      841 cagatgtttg tagaaggtaa tgaaccagat tcatatggca gaaatcgacg agttttcttc
      901 attagtagag atggattttt cttgctggct atggggttta caggaaagaa agctatctac
      961 ttcaaacaaa aatacattga agcattcaac gaaatggaag atgttattcg caagaatact
     1021 gttcctcaaa caattgaaga tatgatgatc tatcaattag aagaaatgaa agatgttaaa
     1081 aaagatgttt ccatgcttaa agatactatg cgaattagcg gacaacaaga gtttgaaatt
     1141 aagcaaaaag gaaatatgaa agttatggga gttctagggg gaaaagaaag ccgagcttat
     1201 gaagaaatca gcaaaaaagt attctcaaaa ttttggtctg aatttaaacg taccttttca
     1261 atcccaagat atggcgagtt acctcgtaag agattcgatg atgctgtttc atttattgaa
     1321 atgtggttgc cagaaactgc gatccgtatg gaaattgatc aactgaacag acaacagaga
     1381 ctttttggtg atgacaatga atagagctga agcgctaaga atagggacgg caattgctaa
     1441 ttgctggtgg aaatactata aaccaatcat cctaagccaa caacatattg acaagcaaaa
     1501 agcatggcaa caaataaaaa agtgactccg ccggcaagca aagagtcaca aagaaaacac
     1561 atcataagga gattttagca tatggaaaaa gaactttcca ctctagatca atatttgatt
     1621 gatcctgatt ggggcaagcc gaaaattgag gaaacaagtg gtcgaaaaat cagacgaaat
     1681 cttttgacga atgaagaact agcttgggat caagatgatt taggcaacca tgtaactatt
     1741 tgggatcatg tttatcttat ccatctatcg aagcattcga ataaacctga atatatttac
     1801 gtcatcgaag atggcttgat tgatgcgcta gaagagtacg acagagataa cttgattgat
     1861 atctcttatt acgga
//
LOCUS       DAFCNJ010000148         1796 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000148 DAFCNJ010000000
VERSION     DAFCNJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.154"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="LNE35_002920"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="LNE35_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK6911994.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAFCNJ010000149         1741 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000149 DAFCNJ010000000
VERSION     DAFCNJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.156"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..1228
                     /gene="aph(2'')-I"
                     /locus_tag="LNE35_002921"
                     /pseudo
     CDS             1..1228
                     /gene="aph(2'')-I"
                     /locus_tag="LNE35_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1212..1604
                     /locus_tag="LNE35_002922"
     CDS             1212..1604
                     /locus_tag="LNE35_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6911995.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1605..>1741
                     /locus_tag="LNE35_002923"
     CDS             1605..>1741
                     /locus_tag="LNE35_002923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911996.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     S"
BASE COUNT          717 a          133 c          298 g          593 t
ORIGIN      
        1 aaaaaaacat tatacagagc cttgggaaga tgaagttttt agagtaatta ttgaatataa
       61 caatgttcct attggatatg gacaaatata taaaatgtat gatgagttat atactgatta
      121 tcattatcca aaaactgatg agatagtcta tggtatggat caatttatag gagagccaaa
      181 ttattggagt aaaggaattg gtacaagata tattaaattg atttttgaat ttttgaaaaa
      241 agaaagaaat gctaatgcag ttattttaga ccctcataaa aataatccaa gagcaataag
      301 ggcataccaa aaatctggtt ttagaattat tgaagatttg ccagaacatg aattacacga
      361 gggcaaaaaa gaagattgtt atttaatgga atatagatat gatgataatg ccacaaatgt
      421 taaggcaatg aaatatttaa ttgagcatta ctttgataat ttcaaagtag atagtattga
      481 aataatcggt agtggttatg atagtgtggc atatttagtt aataatgaat acatttttaa
      541 aacaaaattt agtactaata agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt
      601 tttaaataca aatttagaaa ctaatgtaaa aattcctaat attgaatatt cgtatattag
      661 tgatgaatta tctatactag gttataaaga aattaaagga acttttttaa caccagaaat
      721 ttattctact atgtcagaag aagaacaaaa tttgttaaaa cgagatattg ccagtttttt
      781 aagacaaatg cacggtttag attatacaga tattagtgaa tgtactattg ataataaaca
      841 aaatgtatta gaagagtata tattgttgcg tgaaactatt tataatgatt taactgatat
      901 agaaaaagat tatatagaaa gttttatgga aagactaaat gcaacaacag tttttgaggg
      961 taaaaagtgt ttatgccata atgattttag ttgtaatcat ctattgttag atggcaataa
     1021 tagattaact ggaataattg attttggaga ttctggaatt atagatgaat attgtgattt
     1081 tatatactta cttgaagata gtgaagaaga aataggaaca aattttggag aagatatatt
     1141 aagaatgtat ggaaatatag atattgagaa agcaaaagaa tatcaagata tagttgaaga
     1201 atattatcct attgaaacta ttgtttattg atgataatat ggtttttgat attgataatt
     1261 taaaaggatt tcttaatgat accagttcat ttgggtttat agctaaagaa aataataaaa
     1321 ttataggatt tgcatattgc tatacacttt taagacctga tggaaaaaca atgttttatt
     1381 tacactcaat aggaatgtta cctaactatc aagacaaagg ttatggttca aaattattat
     1441 cttttattaa ggaatattct aaagagattg gttgttctga aatgttttta ataactgata
     1501 aaggtaatcc tagagcttgc catgtatatg aaaaattagg tggtaaaaat gattataaag
     1561 atgaaatagt atatgtatat gattatgaaa aaggtgataa ataaatgaat atagttgaaa
     1621 atgaaatatg tataagaact ttaatagatg atgattttcc tttgatgtta aaatggttaa
     1681 ctgatgaaag agtattagaa ttttatgatg gtagagataa aaaatataca ttagaatcat
     1741 t
//
LOCUS       DAFCNJ010000150         1573 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000150 DAFCNJ010000000
VERSION     DAFCNJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.157"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAFCNJ010000151         1551 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000151 DAFCNJ010000000
VERSION     DAFCNJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.158"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            132..344
                     /locus_tag="LNE35_002924"
     CDS             132..344
                     /locus_tag="LNE35_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911997.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="LNE35_002925"
     CDS             506..1024
                     /locus_tag="LNE35_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBK6911998.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="LNE35_002926"
     CDS             1031..1543
                     /locus_tag="LNE35_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6911999.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          241 g          518 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt a
//
LOCUS       DAFCNJ010000152         1532 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000152 DAFCNJ010000000
VERSION     DAFCNJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.159"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..792)
                     /locus_tag="LNE35_002927"
     CDS             complement(<1..792)
                     /locus_tag="LNE35_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6912000.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT          504 a          297 c          255 g          476 t
ORIGIN      
        1 atacaaccaa caaaactcct cttctgataa agcaacttga taatttgttt gaatcgcttc
       61 tttcaaggaa tacattaagc gaaaattctc actagcaatt actcgctcaa tcagaccttg
      121 aggaatggct atcgtttttc cttgtctcac acgctcaatc gcaatataca aactataata
      181 aaattcagca ggagaagtcc ctgttccaat cgagaaatac tcaaatttat catagaatgt
      241 agcttgcaca agttcatgaa gatcccttgg tggaacaaga gtatggggcg tcatctcccc
      301 ctcataatag aagtctttaa aaaacttgcg aatatttgcc tcttctcctt ttaaatcaag
      361 aggataaaga ctcaattcta agccgtattc ttccaaaact ggttgaatct gcttcaaata
      421 ccgacgaaaa gtcgcttcag ataaatgaaa acgatcaatt agctccgtca tatcttccaa
      481 ttcgccataa aagacattcc ctaacaactc aaataaaatt tcgttctcca gtagttgctc
      541 cttctgcttg agaaaaagaa ccggtctttc cttctgaaaa gaatacccac ttttcgttga
      601 aacaaattga acactttcag aaaagtagtc cttcatttta ttgacatctg tttgaatggt
      661 tcgttcagat acatgattac tctttgctaa ttctcctttt gtggcaagtt gattgcgctc
      721 aaattgattg ataatccgaa accagcgtac aatcgatgga tcaacaataa attttaactg
      781 taaatttttc atcaacaacc ctcctcttag aaaaaaattt catcctatac ctatataatt
      841 acgataccat tttttctcct ttataggtaa tgttttgggg aaggaaaaca agtttctatt
      901 actcacctga taaaatcaat accgtcgact atactgcgga attgtttggg tatagcaaat
      961 aagaccaaat gaaaaaactt ggtcttggat aaaaaatgta accgatttct atttacccaa
     1021 aaagaggata tgctatactt aatgagacaa cttactatca ttcaggtgta gtattgttcg
     1081 gtcgtggtac cccaacaact ttacaatttc ttttgaaacg ttggttgtga ggagggttgc
     1141 caatgaatca cagtggaatg ggaaaggtta cgttctgaaa tagaatgttg caaaatattc
     1201 tagcaatctt atcgatcatt aacttgatct tagagattgc gaacaaagta aaaagcttaa
     1261 aggactagtc ctttaagcca cactcattac cactgccgaa gcagtttcgt aaaaggtgat
     1321 accagcacct tttacctaca cctgaattat agcaagtttt ttggggttag tcaaaatagc
     1381 taataatggt gtagtgcaat ttctttttga aatgttggtt gtatgctaat gaatcccaat
     1441 gaactgggga aggttacgtt ctgaaataga tagaatgttg caaaatattc tagcaatccc
     1501 taggatcatt cacatgacct tagggattgc ga
//
LOCUS       DAFCNJ010000153         1509 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000153 DAFCNJ010000000
VERSION     DAFCNJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.160"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            101..868
                     /locus_tag="LNE35_002928"
     CDS             101..868
                     /locus_tag="LNE35_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6912001.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="LNE35_002929"
     CDS             1060..1458
                     /locus_tag="LNE35_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK6912002.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DAFCNJ010000154         1469 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000154 DAFCNJ010000000
VERSION     DAFCNJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.162"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..695
                     /locus_tag="LNE35_002930"
     CDS             288..695
                     /locus_tag="LNE35_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912003.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="LNE35_002931"
     CDS             709..1110
                     /locus_tag="LNE35_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912004.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..>1469
                     /locus_tag="LNE35_002932"
     CDS             1112..>1469
                     /locus_tag="LNE35_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912005.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKII"
BASE COUNT          562 a          178 c          258 g          471 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataa
//
LOCUS       DAFCNJ010000155         1451 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000155 DAFCNJ010000000
VERSION     DAFCNJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.163"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..625)
                     /locus_tag="LNE35_002933"
     CDS             complement(<1..625)
                     /locus_tag="LNE35_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HBK6912006.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="LNE35_002934"
     CDS             complement(784..1050)
                     /locus_tag="LNE35_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6912007.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="LNE35_002935"
     CDS             complement(1040..1396)
                     /locus_tag="LNE35_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6912008.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DAFCNJ010000156         1440 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000156 DAFCNJ010000000
VERSION     DAFCNJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.164"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            105..1406
                     /locus_tag="LNE35_002936"
     CDS             105..1406
                     /locus_tag="LNE35_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6912009.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAFCNJ010000157         1417 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000157 DAFCNJ010000000
VERSION     DAFCNJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.165"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="LNE35_002937"
     CDS             complement(150..1337)
                     /locus_tag="LNE35_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBK6912010.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAFCNJ010000158         1393 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000158 DAFCNJ010000000
VERSION     DAFCNJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.166"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1393
                     /locus_tag="LNE35_002938"
     rRNA            <1..1393
                     /locus_tag="LNE35_002938"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          374 a          300 c          424 g          295 t
ORIGIN      
        1 aagtctgaag aggagtcaaa tgcttctttt cttaaggaca agctgtgatg gggagggaaa
       61 taatagtacc gaagttcctg atgtcacact gccgagaaaa gcttctagtg agaaaacagc
      121 ggcccgtacc gcaaaccgac acaggtagtc gaggagagaa tcctaaggtg agcgagagaa
      181 ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgatcat
      241 acgatcagcc gcagtgaata ggcccaagcg actgtttatc aaaaacacag gtctctgcaa
      301 aatcgtaaga tgaagtatag gggctgacgc ctgcccggtg ctggaaggtt aagaggagtg
      361 cttagcgcaa gcgaaggtac gaattgaagc cccagtaaac ggcggccgta actataacgg
      421 tcctaaggta gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaacgat
      481 ttgggcactg tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta
      541 cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt gagtgtctgt
      601 accgcatgta caggataggt aggagccgta gaaatcggaa cgctagtttc gatggaggcg
      661 ctggtgggat actacccctg cgttatggcc actctaaccc gcaccactaa tcgtggtggg
      721 agacagtgtc agatgggcag tttgactggg gcggtcgcct cctaaaaggt aacggaggcg
      781 cccaaaggtt ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc agaagggagc
      841 ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga tccggtggtt
      901 ccgcatggaa gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct
      961 cccccaagag tccacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct
     1021 ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca cgcgagctgg
     1081 gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga aatttgagag
     1141 gagctgtcct tagtacgaga ggaccgggat ggacttaccg ctggtgtacc agttgttctg
     1201 ccaagggcat tgctgggtag ctatgtaggg aagggataaa cgctgaaagc atctaagtgt
     1261 gaagcccacc tcaagatgag atttcccatt tctttaagaa agtaagatcc ctgagagatg
     1321 atcaggtaga taggtcagga gtggaagtac agtgatgtat ggagcggact gatactaatc
     1381 gatcgaggac tta
//
LOCUS       DAFCNJ010000159         1302 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000159 DAFCNJ010000000
VERSION     DAFCNJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.169"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..>1302
                     /locus_tag="LNE35_002939"
     CDS             52..>1302
                     /locus_tag="LNE35_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6912011.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DAFCNJ010000160         1296 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000160 DAFCNJ010000000
VERSION     DAFCNJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.170"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            141..398
                     /locus_tag="LNE35_002940"
     CDS             141..398
                     /locus_tag="LNE35_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912012.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1061)
                     /locus_tag="LNE35_002941"
     CDS             complement(630..1061)
                     /locus_tag="LNE35_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBK6912013.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          489 a          165 c          162 g          480 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaattttt atgtaccgcc cctcaa
//
LOCUS       DAFCNJ010000161         1294 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000161 DAFCNJ010000000
VERSION     DAFCNJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.171"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1244
                     /locus_tag="LNE35_002942"
     CDS             <1..1244
                     /locus_tag="LNE35_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK6912014.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAFCNJ010000162         1271 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000162 DAFCNJ010000000
VERSION     DAFCNJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.172"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            265..957
                     /locus_tag="LNE35_002943"
     CDS             265..957
                     /locus_tag="LNE35_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK6912015.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          554 a          144 c          183 g          390 t
ORIGIN      
        1 aattattgac acaacctatt ctggaaactt acaaataaaa gcttattctt cagccacctt
       61 cgggtggtta ttttttataa aaaaatgtaa aaaaatattg atacataaat aaattagttg
      121 tagtaaatag ttttagaatt gaatttctct atccttttta aataattttt tagacaaaaa
      181 acagaaagtg tctaaagaaa gcttgacgaa gaaaataatt tttaataact tatttaagga
      241 aattttttga aggagaagat aaaagtgaga aaaaagatta ttattaatgc tggagtaatt
      301 ataggattga tgttagtaat tttctccgga aagcaatatg ctgataatat tttattatgg
      361 ggaggggaag ataatattga aaagatcaac aacaatttaa ctatattaga cacagctcta
      421 gaaaataaag agcaaaaaat tagtatttta aattcacaat tgtcttcaaa caaccaaact
      481 cttgaacaac taagaactaa tattgaagtt tccaaacaaa aagtggcatc tttagaaaac
      541 gacaagaatc aattagtatc agagaaaact aatttagaaa atcaattgtc agcaaaagac
      601 tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa tagacgaaat taaccaaaaa
      661 gtagctgaaa taaatcgatt aaatagcgat tggaataatc gtttacaaaa tgaaaagaac
      721 aaatttaatg aagaacaatc tactattaat agtttaaacc agcaaattaa tcaaatgttg
      781 agtgaaaaaa ataacattca aaatcaactg caagctactc agcaaaagct agaagaagaa
      841 aagtcaaaaa atagtagttt gaataattac attgaaaagc ttcaaaaagc taaaaatgat
      901 gtagaggata ccgcaaataa agcacaacaa attgtaaatg aacatacaat ccaatagata
      961 tagttttaat ttaacaaatg gtatgtttgg agataatgag atagaatata aatgtcataa
     1021 tattatacaa tctaaacacc gtttcctttt ctatttttga tgacatcagt atatagctac
     1081 tcgaaagagt agtcttttta ttattctcgc ttattattga tatgaaaatt tcgatttgac
     1141 aaaaggtacc gaataatttt gtaatatgaa ttattatcaa gaagtaatta tgatgataac
     1201 atatggaaac tagttagtaa taataacagg tgattaaact tttgaacgac ttgtaattct
     1261 atattttttc t
//
LOCUS       DAFCNJ010000163         1269 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000163 DAFCNJ010000000
VERSION     DAFCNJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.173"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..1103
                     /locus_tag="LNE35_002944"
     CDS             24..1103
                     /locus_tag="LNE35_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6912016.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          378 a          302 c          310 g          279 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaacccatga taacacagtt tcacgcaagg
       61 gaaaacatct ttcctactca gaacgttgcc aaatcgctat cctgaaaaag gaaggctatt
      121 ccaaccgtca ggttgccaaa gtgctgggac gtgttccgca aaccataaac aatgagattg
      181 atcgcggaac catcacccag ttaaaacgcc aaacgcaaaa tggaaaagtg tatgacacct
      241 actattctgt ttatgacgct gatgcaggac aggccttcta tgaaaagcaa cgactgaact
      301 gtggcagacg ccccaaatgg gcgaacacaa atgccttcat tgattgggca gatgacaaaa
      361 tgttaaatga aaaatggtcg ccggacgtcg tggtcggatt tgccagaaaa cacgagctgt
      421 tcgattgtgc catcattcct ggcacaaccg ctctttatca atggattgac cgcggaatca
      481 tgcgaacaaa gaacatggac ttgcttgaaa aactgtcccg taaaatcaaa gcggtccgcc
      541 caggacaccg tcccaacaag cgtgttctcg ggacgtcaat tgaagaacgc cctaaagaag
      601 ttgaaacacg ggaaacgttc ggtcattggg aaatcgacac ggtagtggga aacaagacca
      661 agtcggatgc cgtgctgctg acgctggctg aacggcagac acgctttgaa gtcatcctaa
      721 agttgaacgg gaaggacacc gaatcggtgg accaagcggt tcaacagcta agagaacggg
      781 ctggcgctaa cttcgatcaa ttatttaaga ccgttacatc cgacaatggc tcggagtttt
      841 ctgggctaca cgatgctctg caagatgtag ctgacgtcta tttcagccac ccctatgctt
      901 cttgggagcg tgggacgagc gaaaaccagc acaaattaat tcgccgtttc gtccccaagg
      961 gaagcccgat tagtgaggtg agcgagaacc agattttgcg gatccagcgt tggatgaacg
     1021 attacccgcg gaaaattttg ggttatgcga caccacatga tacattcgtc caggcattca
     1081 agcaagaacg gttggtggct tgacccatta ttccttgata acgatcctca ctcgggacat
     1141 ggcttcatgt ctggaaagct ggcctggggc cagaatcgtc tgaagactat catacctcag
     1201 ttcattatca aggcctcact gatttagaat aacgaggcag ttacggtatc aaacaaatga
     1261 gttacctgt
//
LOCUS       DAFCNJ010000164         1265 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000164 DAFCNJ010000000
VERSION     DAFCNJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.174"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..1103
                     /locus_tag="LNE35_002945"
     CDS             24..1103
                     /locus_tag="LNE35_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6912017.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          335 a          343 c          356 g          231 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaaaccatga taacacagtt tcacgcaagg
       61 gaaaacacct aacctactca gaacgttgcc aaatcgccat cctgaaaaag gaaaactatt
      121 ccaaccgtca ggttgcggat gtcctgggct gcgtgccgca gaccataaac aacgaaatca
      181 aacgcgggac cgttactcag cttaaccgcc agaatcagaa cgggaaggtc tacgactatt
      241 atcatgacgt ctacgacgcc gatgccgggc aggcggccta cgagaagaac cgcttgaact
      301 gcggcagacg gcccttatgg gccgacacgg acgccttcat tgaatgggcg gacgacatga
      361 tgctgttgga aaaatggtcc ccggacgtgg tggtgggctt tgccagaaaa cacgaattgt
      421 tcgacccggc cattatccct gggaccacca ccctctatca gtggatcgac cgtggcatca
      481 tgaaaacgaa gaatatggac ctgctagaaa aactgtcccg caaaccgaaa tcgtcccgtc
      541 atggcaaccg cccgaataaa cgggtgctag ggaaatccat tgaagagcgc ccggaggcgg
      601 ttggtacccg gaaaaccttc ggccattggg aagtcgacac ggtcgtagga aacaagacca
      661 agtcggatgc cgtgctgctg acgttggtgg aacggcagac acgcttcgag gtcattctga
      721 agctggccgg gaaggatgcc gaatcggtca accaagcggt ccgacagctg agagaacggg
      781 ctggtgcgta cttcggccag ctgttcaaga cggttacctc ggacaacggc tcggagttcg
      841 cgggactgca cgatgccttg caggatgtgg caaacatcta cttcagccac ccctatgctt
      901 cctgggagcg cgggacgagc gaaaaccaac acaagctgat tcgccgtttc atccccaagg
      961 gcagcccaat cggcaacgtg agcgaaaacc aaatcttgcg gattcagcgt tggatgaacg
     1021 attacccgcg aaagattttg ggctatgcaa cgccgcatga cacgttcgtc caggcattta
     1081 ggcaggaacg gttgacggct tgacacgtgg gcccttgaca acgagcctcc ctcggcatgg
     1141 cttgtgtctg gaaagctggc cggggccagc cagtcgggag actaccatgc ctcagctcat
     1201 tgtcaagggc ccactcgatt agaaaaatta gacggttggt ggtaacgagc aaccgggtca
     1261 ccatg
//
LOCUS       DAFCNJ010000165         1246 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000165 DAFCNJ010000000
VERSION     DAFCNJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.175"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="LNE35_002946"
     CDS             200..742
                     /locus_tag="LNE35_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912018.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="LNE35_002947"
     CDS             898..>1246
                     /locus_tag="LNE35_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBK6912019.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAFCNJ010000166         1211 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000166 DAFCNJ010000000
VERSION     DAFCNJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.176"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1211
                     /locus_tag="LNE35_002948"
     CDS             <1..>1211
                     /locus_tag="LNE35_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBK6912020.1"
                     /translation="QKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVV
                     IDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     IRIFLINQLIN"
BASE COUNT          402 a          239 c          231 g          339 t
ORIGIN      
        1 ttcagaagaa aaagacgttg gtcgtcgatg ctgttctctc gcctactcct cgtgcttgta
       61 gaagttgtgg ttctactgtg gtagatggaa acgggaaagc aattatagtg aaaaatggaa
      121 aaaaggaaac gattgtccgt tttgaacaat acaatcatat gcctttggtt atgcgcctaa
      181 aaaagcaacg ctatacctgt aaaaactgtc gcacccattg gacggctcaa agttattttg
      241 tccaacccag acattcaatc gcaaatcatg ttagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa gctgtcaggt atctttgacc accgttattc
      361 gtacattgaa agagtttaaa agctatttac caaagcaatc taagaagatc ctccccagag
      421 tattgatggt tgacgaattt cgttcgcatg cttccataga agataagatg agctttattt
      481 gcgcggatgg cgaaacagga aaattaatag atgttttgcc tacgcgtaaa ttacctcgat
      541 taacaagcta tttcttaggt tgtaccaatc cagaagaagt agaattcttg gtgacagaca
      601 tgaacgccgc ctacttccag ctcaccaaac gtgttctgcc aaatgcgaaa gtcgtgattg
      661 atcggtttca tattgtcaaa cacatgaatc aagcgttcaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagcc ggacagaaaa gccaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 tccgagcacc aaaataccca tttcttactg aagcaatgat gattgatcga ttgcttgagt
      901 tttctccgcc cctaaaggag gcgtatccct tttttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgaca
     1021 gctttcggga aaagcttcaa aaccttctga cctatgaaga aggcatcacc aacgcaatga
     1081 tctatcctta ttccaatgga aaaatagaag cgaagaatac ccacataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc
     1201 aattaatcaa t
//
LOCUS       DAFCNJ010000167         1193 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000167 DAFCNJ010000000
VERSION     DAFCNJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.178"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1076
                     /locus_tag="LNE35_002949"
     CDS             <1..1076
                     /locus_tag="LNE35_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK6912021.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAFCNJ010000168         1167 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000168 DAFCNJ010000000
VERSION     DAFCNJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.179"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1167
                     /locus_tag="LNE35_002950"
     CDS             <1..>1167
                     /locus_tag="LNE35_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HBK6912022.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSF"
BASE COUNT          383 a          230 c          227 g          327 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttg
//
LOCUS       DAFCNJ010000169         1143 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000169 DAFCNJ010000000
VERSION     DAFCNJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.180"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="LNE35_002951"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="LNE35_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBK6912023.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DAFCNJ010000170         1134 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000170 DAFCNJ010000000
VERSION     DAFCNJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.181"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            13..423
                     /gene="spx"
                     /locus_tag="LNE35_002952"
     CDS             13..423
                     /gene="spx"
                     /locus_tag="LNE35_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK6912024.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            437..793
                     /locus_tag="LNE35_002953"
     CDS             437..793
                     /locus_tag="LNE35_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBK6912025.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          396 a          170 c          217 g          351 t
ORIGIN      
        1 atgagtgaga aaatgataaa agtatatact tcgcctagct gtacttcctg tagaaaagca
       61 agagcatggt tgcaagcgaa tcaacttgaa tttgaagaga agaatatttt tgccgaaccg
      121 ttaacagaaa gtgagatcaa aaagatcttg gcagcgactg aaggaggagt tcaagatatt
      181 atttccactc ggtcgaaaat ttacgaaaag ctgggcatag attttaacga attaacttta
      241 aagcaaatga tcgtcttatt caaagaatat ccttcattat tgagacgacc tattttgatt
      301 gatgaaaaaa gaatactaat cggatttaat gaagatgaga tccggtcatt tgtccctcgt
      361 gaaattcgat gtgttgccag aaatctgatc tatttacaaa acaataacat cttaaattca
      421 tagggaggga gccttcgtgg aagtgaaagt ctatggatca gcgcactgtg taacaagtct
      481 gaaggcgaaa caatggttag aacaccatca attgccttat caatttgttg acttagaaga
      541 acgcgatcta agtacagaag aagcagaaga attatgcagt tttgaagaag tgcaagcaga
      601 acagctattt gcgacttggt ctgaggcttt tcacaacatt gcgattgacc tttcctgtgt
      661 aggaaaaaaa caactgattt ttttatgcac acaacatacc aatctcctca gacgtccttt
      721 gattattatc gatgaagttc tatttattgg atacaatgag cagttgatgg aacagcttat
      781 cttaaataaa tagactttaa ctgttttaat caagtcactg agcatttcat acagttgtaa
      841 cagtcgaaca cttagcatca agcgtgttat gtgttctttt tttgacttta tttttctact
      901 atagagacat gctcaattta tgtggtcaaa attcaaatct aggtggggat atagagttaa
      961 aaatatattg acatgtgtaa atttacatga tatattcatt tctgttgttt taagtagcaa
     1021 agcaataaaa tagtatttta aaaaacatat aaaatagttg ttgacatgtt agaaatgaca
     1081 tgataagata actgagttgt taaaaaagaa ccacttgcga agcttgattc tatc
//
LOCUS       DAFCNJ010000171         1114 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000171 DAFCNJ010000000
VERSION     DAFCNJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.182"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..897)
                     /gene="vanY"
                     /locus_tag="LNE35_002954"
     CDS             complement(<1..897)
                     /gene="vanY"
                     /locus_tag="LNE35_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBK6912026.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          399 t
ORIGIN      
        1 agtatgtgtt gatccgggaa acactgtcac aattacaccg tctatattgt ttcctgatat
       61 ctcataacga agattagtcg gcacatgaat ggtggtattt ttagtaacag gataataaaa
      121 gatctcatat ttttccccat ttacactaac agaaatggtt ttttcttctt ttaggtaatc
      181 catatattcc tcgagaacga aattcttttc tttcataatc gcactatgtg gtaaaccaac
      241 atagcgaata tgccatggtt catattgaat tcctgttaac tctgttttgt cctctggata
      301 acgtaaaatg aacccgtatt tccaagcatt ttcttctatc cactttcctt caggggctcg
      361 ttccattttc gtcaagcttg atcctacatc tagtgataaa cctgaattat gctcactata
      421 acctgctggt aaggcatact cagcccccat ttcttggtaa agcacacttt gctcatcaaa
      481 gtctcgatag ccactattaa taataaaatg actaacgcca ccctttacag catcattgac
      541 catctctgaa aatttttgtg ctatttcttt tgacatataa atattactat caagcaaccc
      601 gtatccattt attaattcgt catgtttaga taaattcacg atatctgact tcacactttc
      661 ttggcgaaca ggatatttac tattgattaa tagcagattt ccttgataaa cctgttcttc
      721 tgtaattgtt ttctcttggg tattttcaga agtcccacta ttttctaaat gttctttggg
      781 attttgatca taattttgaa attcgacttt ttcctgagaa aacagtgctt cattaacgta
      841 gtcataacct aagtatatta agaataacaa taacaataaa aaaaacaact tcttcatttt
      901 cagtctcctc cttaactata tagttggaag tatgaaatat ttctagaatc ttccccttaa
      961 acggcacaat ctcgtttgag attgtgccgt tttgccccgt aaattacagt caattatttc
     1021 gccctactgg atttactatt atcaccaatg tagatgaatt ttcataagtg atacccatgc
     1081 tgaatttaac gaggctgaac tgcctgttga caat
//
LOCUS       DAFCNJ010000172         1105 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000172 DAFCNJ010000000
VERSION     DAFCNJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.183"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1087
                     /locus_tag="LNE35_002955"
     CDS             <1..1087
                     /locus_tag="LNE35_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6912027.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DAFCNJ010000173         1069 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000173 DAFCNJ010000000
VERSION     DAFCNJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.184"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..969
                     /locus_tag="LNE35_002956"
     CDS             <1..969
                     /locus_tag="LNE35_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK6912028.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAFCNJ010000174         1062 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000174 DAFCNJ010000000
VERSION     DAFCNJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.185"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..963
                     /locus_tag="LNE35_002957"
     CDS             4..963
                     /locus_tag="LNE35_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBK6912029.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          369 a          200 c          210 g          283 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DAFCNJ010000175         1058 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000175 DAFCNJ010000000
VERSION     DAFCNJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.186"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..992)
                     /locus_tag="LNE35_002958"
     CDS             complement(36..992)
                     /locus_tag="LNE35_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912030.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DAFCNJ010000176         1051 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000176 DAFCNJ010000000
VERSION     DAFCNJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.187"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="LNE35_002959"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="LNE35_002959"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBK6912031.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAFCNJ010000177          997 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000177 DAFCNJ010000000
VERSION     DAFCNJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.188"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..913)
                     /locus_tag="LNE35_002960"
     CDS             complement(26..913)
                     /locus_tag="LNE35_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK6912032.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DAFCNJ010000178          994 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000178 DAFCNJ010000000
VERSION     DAFCNJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.189"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>994)
                     /locus_tag="LNE35_002961"
     rRNA            complement(<1..>994)
                     /locus_tag="LNE35_002961"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAFCNJ010000179          988 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000179 DAFCNJ010000000
VERSION     DAFCNJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.190"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..704
                     /locus_tag="LNE35_002962"
     CDS             <1..704
                     /locus_tag="LNE35_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK6912033.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          335 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataa
//
LOCUS       DAFCNJ010000180          978 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000180 DAFCNJ010000000
VERSION     DAFCNJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.191"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            47..274
                     /locus_tag="LNE35_002963"
     CDS             47..274
                     /locus_tag="LNE35_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBK6912034.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            343..609
                     /locus_tag="LNE35_002964"
     CDS             343..609
                     /locus_tag="LNE35_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK6912035.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            599..868
                     /locus_tag="LNE35_002965"
     CDS             599..868
                     /locus_tag="LNE35_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6912036.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT          346 a          157 c          205 g          270 t
ORIGIN      
        1 tcgcaatccc taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa
       61 ccaaaatacg aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac
      121 tgatactact gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat
      181 ccctgaaaat tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag
      241 attttgcgat accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt
      301 tgtactaacg ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg
      361 aacggaggta gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa
      421 gaactggtat tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat
      481 gctgtactgg ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa
      541 gtttccaaag agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat
      601 gaattataaa gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa
      661 ccaagctaaa ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg
      721 tcagcacgga aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa
      781 ctatcgaata ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg
      841 acatagaagt acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt
      901 caatgtataa tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg
      961 gttggtttct gctttttt
//
LOCUS       DAFCNJ010000181          907 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000181 DAFCNJ010000000
VERSION     DAFCNJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.192"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..>907)
                     /gene="menA"
                     /locus_tag="LNE35_002966"
     CDS             complement(132..>907)
                     /gene="menA"
                     /locus_tag="LNE35_002966"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBK6912037.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT          324 a          185 c          159 g          239 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaat
//
LOCUS       DAFCNJ010000182          904 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000182 DAFCNJ010000000
VERSION     DAFCNJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.193"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            265..888
                     /locus_tag="LNE35_002967"
     CDS             265..888
                     /locus_tag="LNE35_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6912038.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DAFCNJ010000183          875 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000183 DAFCNJ010000000
VERSION     DAFCNJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.194"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..>875
                     /locus_tag="LNE35_002968"
     rRNA            2..>875
                     /locus_tag="LNE35_002968"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          265 a          174 c          260 g          176 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      361 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      421 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      481 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      541 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      601 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      661 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      721 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      781 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      841 ctctccgaaa tagctttagg gctagcctcg gaatt
//
LOCUS       DAFCNJ010000184          811 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000184 DAFCNJ010000000
VERSION     DAFCNJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.195"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..672
                     /locus_tag="LNE35_002969"
     CDS             <1..672
                     /locus_tag="LNE35_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6912039.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAFCNJ010000185          803 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000185 DAFCNJ010000000
VERSION     DAFCNJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.196"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            129..>803
                     /locus_tag="LNE35_002970"
     CDS             129..>803
                     /locus_tag="LNE35_002970"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6912040.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNA"
BASE COUNT          240 a          155 c          147 g          261 t
ORIGIN      
        1 gctctataat ttctaggact gatggaaatt ttctatctgt cctagttttt ttgtaaaaaa
       61 acaaaaagac atacaaaatt tccggtagaa tagagtctcc tacaacatca ttctggggga
      121 attcttgtat gtccatgaat cattttatca aacaactctt tactattaaa gactcaaata
      181 tccaaattca atcctttgaa gaaacttttt ataaaagcag aaaaagtatc gttattgagg
      241 gaactctgat ccgtacctac agacgttgtc cgtgttgtaa agattctaca aagcaaatcg
      301 ttaaaaatgg caagaagatc tctatgattc tcctgaatcg tagcggtaat aaacggacct
      361 atcttcggtt gaagaaacag cgatacctct gccgagcatg taaaaaatat tttaccgctc
      421 ggacgtatct cgttacccca ttctgcttta tttctaagca gattcattac aagatcttag
      481 aagaattgac agagcgtcaa tctataaaag caattggtaa acattgcgat gtttctgtga
      541 caactgttca gcggacattg tccgccttgt catcagatat ccgtcagaat cttgagtatt
      601 tacctcggtg tctattattc gatgagtttc gttctctttc tacatggcac ggtaaatttt
      661 ctttttcttg tatggacggt gagaccggga aactgtttga gatcttacct agccgccgta
      721 aaaaagatct cgttgctttt ttcatgcgtt tttctctcaa agcgaggttg ggtgtacatt
      781 acatcgtaac ggatatgaac gct
//
LOCUS       DAFCNJ010000186          736 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000186 DAFCNJ010000000
VERSION     DAFCNJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.197"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..734
                     /locus_tag="LNE35_002971"
     CDS             <1..734
                     /locus_tag="LNE35_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBK6912041.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          318 a           94 c          122 g          202 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagtt
//
LOCUS       DAFCNJ010000187          730 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000187 DAFCNJ010000000
VERSION     DAFCNJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.198"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(91..>730)
                     /locus_tag="LNE35_002972"
     CDS             complement(91..>730)
                     /locus_tag="LNE35_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HBK6912042.1"
                     /translation="RRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVT
                     DLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHT
                     DRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQ
                     LRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          200 a          170 c          139 g          221 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt
//
LOCUS       DAFCNJ010000188          714 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000188 DAFCNJ010000000
VERSION     DAFCNJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.199"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..448)
                     /locus_tag="LNE35_002973"
     CDS             complement(<1..448)
                     /locus_tag="LNE35_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6912043.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          216 a          124 c           95 g          279 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aacc
//
LOCUS       DAFCNJ010000189          703 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000189 DAFCNJ010000000
VERSION     DAFCNJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.200"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          297 a          106 c          122 g          178 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgt
//
LOCUS       DAFCNJ010000190          661 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000190 DAFCNJ010000000
VERSION     DAFCNJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.201"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..126)
                     /locus_tag="LNE35_002974"
     CDS             complement(<1..126)
                     /locus_tag="LNE35_002974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK6912044.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPH"
     gene            complement(159..>661)
                     /locus_tag="LNE35_002975"
     CDS             complement(159..>661)
                     /locus_tag="LNE35_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HBK6912045.1"
                     /translation="GIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGVLVILEGGMII
                     DTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVMAPLGDLMNVS
                     RDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFAILTLVSAVLL
                     YIATLF"
BASE COUNT          241 a          132 c          117 g          171 t
ORIGIN      
        1 atgcggagca cctccccaaa gttcaacaga agaaatacca attttctgtt gataagcgaa
       61 aaattctttg agagaataaa ataaatagtg gatattcatt cctgctaatt gatttatttt
      121 aatcataact attgcctcct attaatggat gattaagact aaaagagtgt agcaatgtat
      181 aacaaaacag cagaaacaag ggttaagatg gcaaatattt tgaagccaac cttaaaccat
      241 ttagagaaat taatctctgc gatggctaat cctgccagta atacaccact agttggggag
      301 ataatattca ttaaaccatt tccaaactgg ttacttagaa caacaacatc acgacttaca
      361 ttcattagat cacccaatgg tgccataacc ggcatcgtta atgccgcagc tcctgaggag
      421 gatggaacaa aaaaggaaac aattacttga gaaatgagta gaatcgtagt gaacaggaat
      481 tttggaagac cacctaaaag gttagccaag ctgtttagaa tagtgtcaat aatcattcca
      541 ccttccaaga ttaccaagac tcctctagct agcccaatga ttacagcagc ataggcgaaa
      601 tctgaacagc cttcgacaaa agcttcagca atttcctttt gagaaaatct agcgatgccc
      661 c
//
LOCUS       DAFCNJ010000191          650 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000191 DAFCNJ010000000
VERSION     DAFCNJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.202"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..>650
                     /locus_tag="LNE35_002976"
     CDS             51..>650
                     /locus_tag="LNE35_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBK6912046.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLY"
BASE COUNT          235 a          107 c          129 g          179 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat
//
LOCUS       DAFCNJ010000192          649 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000192 DAFCNJ010000000
VERSION     DAFCNJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.203"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>649
                     /locus_tag="LNE35_002977"
     CDS             <1..>649
                     /locus_tag="LNE35_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6912047.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAFCNJ010000193          608 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000193 DAFCNJ010000000
VERSION     DAFCNJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.204"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>608
                     /locus_tag="LNE35_002978"
     CDS             <1..>608
                     /locus_tag="LNE35_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK6912048.1"
                     /translation="DQLKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPS
                     RSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIA
                     KVGYNSTKKSYFYGLKIHMIVTKTGFPVTYSITNPGVHDVKVLETLSEEANLPNILGD
                     KGYIRHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQR"
BASE COUNT          207 a          115 c          110 g          176 t
ORIGIN      
        1 ctgatcaact gaagcaacgc gatacagtga ttattgcttg tgttatatgg ggcataatta
       61 atggctatac tagccaaaga gccacgtata gagcggtttg ttccgtctta tttcctaatg
      121 gtgactttcc tagtaggagt cgattcactc gcttaagttc aaacttagct tacactatca
      181 agattattcg atactttttt atcaagaagc tcaccaaagg tgaactagtc ggaattatag
      241 atagttttcc tagtccatta tgtaaaccag ttagaaatag acaagcaaaa ctattgaatc
      301 aaatagccaa agttggctat aactcaacaa aaaaatctta tttttatggt cttaaaattc
      361 atatgatcgt tactaagact ggctttccgg ttacttactc aatcacgaat ccaggtgtcc
      421 atgacgtgaa agtgttagag actttatcgg aagaagcgaa tcttcccaat atcctagggg
      481 acaaaggata tattaggcat aaaatccatg aaaagcttgc ccttaagggc attactatat
      541 ccgttccgcc acgtaaaaac atggataaat cagaaaaact agaccacagc ttacttggaa
      601 aacaaaga
//
LOCUS       DAFCNJ010000194          590 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000194 DAFCNJ010000000
VERSION     DAFCNJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.205"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          203 a           67 c           99 g          221 t
ORIGIN      
        1 tgacataatt gcattgtatc cgctttcttt gtgtttgtca ataggaaaaa tttatttctc
       61 taaaaaatat aaataatctg cagtgaaact tgagtacata tactaatttc gaaaaatgcc
      121 tatgatttaa ataattatgg tgctgggatg aaggtttata agtttgtctt gatttgttta
      181 gaagaaatcg tttttgcaca cagttgaatt aggaaactac tggagtggaa aataaagtga
      241 tttgacgggt agatgggtgc acttaaattt gaaaatggct taaaatgctt tattttgaat
      301 tgcagaattt cttttagtac gcaatttatg gtcttaaaac tgattaaaaa gtgatacatt
      361 gattttaata caatttatga tacaaaaaat gacctccttt cttaaataaa agaggtcatt
      421 ttgttttgtc tcataatcga ttgtttttga tacagtagtt atattcagaa aaaataaaga
      481 ctcttctgag tttataatcc tttggattat atacggatac agatgtagac ataaaacaag
      541 ctcaattttc aaaaatggca ttactcacct ttttggtgaa ttatttgtat
//
LOCUS       DAFCNJ010000195          560 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000195 DAFCNJ010000000
VERSION     DAFCNJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.206"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          187 a           88 c           87 g          198 t
ORIGIN      
        1 gaagagtatt atacaagcta aagcttttct tcattgccac tcaaatgagt ggctttttta
       61 tgtatccttt tatggattaa tgaaaggatg tttcacatag ttatacttct gtatatttga
      121 aaagttttac tttgattttt aaatagaaag acatttgggt taaattgtga gataataata
      181 aagaagagtt taaagcgcac cccaaaccac ttccccataa gtgtgttacg ctttaaactc
      241 ttttatattt gaagccatta aaaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttacaattg gagtggtagt taattagtga cttatttttg attttatagc actgatacta
      361 taaaatatag atatcatcat attacacaat cttaatacta acttaaaaaa tatctccttt
      421 cacaagtatg gtgataaaat tcgttccggg ctaccttttt aggtagccta ctttaatctt
      481 tatacctttc tggatcaacg aaagtatact ttatatagtc ataacgccga tgatcgctac
      541 gtgcgtccgg cacgtcagtc
//
LOCUS       DAFCNJ010000196          557 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000196 DAFCNJ010000000
VERSION     DAFCNJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.207"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            69..>557
                     /locus_tag="LNE35_002979"
     CDS             69..>557
                     /locus_tag="LNE35_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBK6912049.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT          208 a          102 c           92 g          155 t
ORIGIN      
        1 caaaaaagga agtcccttct gtagaataaa gttaacgaca accaattcac agaaaagagg
       61 acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcgatt
      121 taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta cggactgaat
      181 taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca ggtaattcga
      241 gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca ttggaaatac
      301 ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa agaacaaacg
      361 atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg tctgaaattg
      421 ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg tccaacatga
      481 ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat gataagtatg
      541 tcgctatttt tatggac
//
LOCUS       DAFCNJ010000197          544 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000197 DAFCNJ010000000
VERSION     DAFCNJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.208"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..360
                     /locus_tag="LNE35_002980"
     CDS             <1..360
                     /locus_tag="LNE35_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBK6912050.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
     gene            366..>544
                     /locus_tag="LNE35_002981"
     CDS             366..>544
                     /locus_tag="LNE35_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912051.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTV"
BASE COUNT          202 a           89 c          116 g          137 t
ORIGIN      
        1 cgaaaccaac cagttgttaa accaaatgca atgcaaatca ataactcatt gaagcgatgc
       61 tttgtgaaag cattggctct acacggactg ggattatatg tatttcaagg ggaagatatt
      121 ccaacaccac ctagaatcga tacaaagaaa ttaaacatgc tagagacgat tctagaagct
      181 ttcaatgagc agatgggtaa agatatgacc aaaaccttaa tcgaatatgt taatgagcag
      241 acagataaat tagggctctt agctgataac gttgaaacta ttgaacagtt aagctatgag
      301 caatgtgcct tgatggagcg agcaatagca gctaagagaa aagaattaga taagaagtga
      361 tatgagtgtt taaaccatta atcgattcat attcagcggt tctgaaaaag ttcaaaggaa
      421 aagacataag cgcaaccatc aatgaggaag tgaacattga tcgactaaag acgatgtatg
      481 acggctacga tggtgatcga gtcattgaaa ttcgttttat tgatcctaga cgtttcaccg
      541 taca
//
LOCUS       DAFCNJ010000198          542 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000198 DAFCNJ010000000
VERSION     DAFCNJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.209"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..75
                     /locus_tag="LNE35_002982"
     CDS             <1..75
                     /locus_tag="LNE35_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBK6912052.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDENE"
     gene            249..>542
                     /locus_tag="LNE35_002983"
     CDS             249..>542
                     /locus_tag="LNE35_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912053.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYG"
BASE COUNT          213 a           94 c          105 g          130 t
ORIGIN      
        1 ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacaaag acttttcggt
       61 gatgaaaatg aatagagctg aagcgctaag aatagggacg gtaattgcta atcgctggtg
      121 gagacacaat aaaccaaaca tcctaagcca acaacatatc gaaaagcaaa aagcatggca
      181 acaaataaaa aagtgactca gccggcaagc atagagtcac aaaacatatc taaggagaat
      241 tttagcatat gaataaagaa ctttccactt tagatcaata tttgactgat tctgaatggg
      301 gcaagtcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacgaacg
      361 aagaactatc atgtgatcaa gatgacttgg gaaattttgt gagtatttgg gatcatgttt
      421 accttatcca tctatcaaaa cattcaaata aacctgaata catctatgtc atcgaagatg
      481 gcttgattga tgcgctagaa gagtacaaca gagataactt gattgatatc tcttattacg
      541 ga
//
LOCUS       DAFCNJ010000199          530 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000199 DAFCNJ010000000
VERSION     DAFCNJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.210"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            312..428
                     /locus_tag="LNE35_002984"
     CDS             312..428
                     /locus_tag="LNE35_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HBK6912054.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          223 a           50 c           70 g          187 t
ORIGIN      
        1 taataaagaa ttgaacttct gagaaaacaa agaatctttt ttcttttttt gaattgactc
       61 atggattggc gttaaaattt tattttgaaa aagttatttc tggtttaact caaaaaaaga
      121 gatatgaatc taatagtgat agaaaggatg ttatttttca tgaaatggtt gttttagatt
      181 taatacatat aactaatgaa tgctattgaa tgtatttaaa ctaattaaca aaagaatttg
      241 aattgttaat aaaatccaat aaaatatatt aataaagtag atagaaataa aaaaacaaag
      301 actaaacata tataattgga ttaaacgcaa tatctgcaaa atattgtaaa tttaaagcct
      361 ttgtagaaaa atttactgaa agacaaactg ataagattat tcagtatcat attacctata
      421 ctgattaaaa ttttttgaaa cgggatacta aataaaaaat ttatttataa cagaactaat
      481 tatttaatgc ggatgcgcca atacttggtc ctgcaagagc tatttatttt
//
LOCUS       DAFCNJ010000200          528 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000200 DAFCNJ010000000
VERSION     DAFCNJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.211"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..90)
                     /locus_tag="LNE35_002985"
     tRNA            complement(5..90)
                     /locus_tag="LNE35_002985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="LNE35_002986"
     tRNA            complement(107..178)
                     /locus_tag="LNE35_002986"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="LNE35_002987"
     tRNA            complement(190..262)
                     /locus_tag="LNE35_002987"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="LNE35_002988"
     tRNA            complement(280..361)
                     /locus_tag="LNE35_002988"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="LNE35_002989"
     tRNA            complement(369..441)
                     /locus_tag="LNE35_002989"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="LNE35_002990"
     tRNA            complement(445..517)
                     /locus_tag="LNE35_002990"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAFCNJ010000201          526 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000201 DAFCNJ010000000
VERSION     DAFCNJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.212"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="LNE35_002991"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="LNE35_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBK6912055.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="LNE35_002992"
     CDS             complement(349..>526)
                     /locus_tag="LNE35_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912056.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAFCNJ010000202          514 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000202 DAFCNJ010000000
VERSION     DAFCNJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.213"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(107..>514)
                     /locus_tag="LNE35_002993"
     CDS             complement(107..>514)
                     /locus_tag="LNE35_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBK6912057.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DAFCNJ010000203          510 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000203 DAFCNJ010000000
VERSION     DAFCNJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.214"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..510
                     /locus_tag="LNE35_002994"
                     /pseudo
     CDS             1..510
                     /locus_tag="LNE35_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           88 c           92 g          118 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag
//
LOCUS       DAFCNJ010000204          502 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000204 DAFCNJ010000000
VERSION     DAFCNJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.215"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            338..>502
                     /locus_tag="LNE35_002995"
     CDS             338..>502
                     /locus_tag="LNE35_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK6912058.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIAC"
BASE COUNT          177 a           89 c           74 g          162 t
ORIGIN      
        1 tctgtactga aatcaaggta ttattgggaa tcccagctta aatcatagat accgtaaggg
       61 attttattct ttatttaaaa ctttgcaaca gaaccttttg ccatatctac tccccctcta
      121 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
      181 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
      241 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
      301 gagtagtctc aaaactccca agaaatgagg actacatatg cgttcatcaa aatatactga
      361 acattataca gatactttta taacattcaa ggaaattatg agaactgttt ctaacatata
      421 ccacaattgt gtaccagata agattaaaaa ccgaagaaat actgatcaac tgaagcaacg
      481 cgatacagtg attattgctt gt
//
LOCUS       DAFCNJ010000205          485 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000205 DAFCNJ010000000
VERSION     DAFCNJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.216"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            3..>485
                     /locus_tag="LNE35_002996"
     CDS             3..>485
                     /locus_tag="LNE35_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBK6912059.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     D"
BASE COUNT          128 a           84 c          111 g          162 t
ORIGIN      
        1 aaatggaaac aatgaaaaga aaattcacct tcccacatag ttatacattg atttttatct
       61 taattatttt ggctgcaatt ttaacttgga taattcctag tggtcagttt gagacagttg
      121 ttgaaaacgt cgcaggtatg gaaaaaacaa cggttgttcc gggaacctat caccaaatcg
      181 ataaggcaga ttatggacaa ggtttttcct ctatactaat ggcaccagct gaagggatta
      241 tttcagctgt tgaggttgtt gcttttgttc tcatcgttgg tggtgccttc gggattatcc
      301 taaaaacggg agccatcgat cgagggctgt ttaccttagc aaaagttctg ggagacaagg
      361 ggatcttagt tattccgatg tcgatgattc tattcagctt aggaggatca acctttggca
      421 tgagcgagga agtcattccc ttatttgcgg tgtttatttc tctgatgttt tctcttggct
      481 atgat
//
LOCUS       DAFCNJ010000206          452 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000206 DAFCNJ010000000
VERSION     DAFCNJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.217"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>452
                     /locus_tag="LNE35_002997"
     CDS             <1..>452
                     /locus_tag="LNE35_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912060.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAFCNJ010000207          448 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000207 DAFCNJ010000000
VERSION     DAFCNJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.218"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            87..437
                     /locus_tag="LNE35_002998"
     CDS             87..437
                     /locus_tag="LNE35_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6912061.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          182 a           47 c           75 g          144 t
ORIGIN      
        1 tgttgggtaa attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt
       61 tttggtagtt ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag
      121 tcatacatga attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag
      181 atattactga tgttttattt caggtatata aaaaaattga tacagcaaaa tatcctgaaa
      241 ttcttgtaaa taaattagtt aattatattt acatcgtggg ttttgataat aaaatacgct
      301 ttattgataa tgatgaaaaa ttattaatag aattagggga tatttctaaa aaagctggaa
      361 ttaatagtaa atacaaagca aactttacag ataaatcgca attttatagt tattcagaaa
      421 caatgcctca acgataaaat atagtata
//
LOCUS       DAFCNJ010000208          415 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000208 DAFCNJ010000000
VERSION     DAFCNJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.219"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..339)
                     /locus_tag="LNE35_002999"
     CDS             complement(<1..339)
                     /locus_tag="LNE35_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6912062.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAFCNJ010000209          400 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000209 DAFCNJ010000000
VERSION     DAFCNJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.220"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..390
                     /locus_tag="LNE35_003000"
     CDS             <1..390
                     /locus_tag="LNE35_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6912063.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          156 a           72 c           60 g          112 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt
//
LOCUS       DAFCNJ010000210          399 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000210 DAFCNJ010000000
VERSION     DAFCNJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.221"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DAFCNJ010000211          375 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000211 DAFCNJ010000000
VERSION     DAFCNJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.222"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..132)
                     /locus_tag="LNE35_003001"
     CDS             complement(<1..132)
                     /locus_tag="LNE35_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK6912064.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DAFCNJ010000212          365 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000212 DAFCNJ010000000
VERSION     DAFCNJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.223"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          144 a           47 c           45 g          129 t
ORIGIN      
        1 ttttacactc tctgattgac gtcataaaaa gtagctttga attcataatc gtttatataa
       61 cgattaatag aaaacaatat agtatttagt gttcatacaa tgattctaag atcattttga
      121 attaatcagt cttatagttg atttctaaat tgtcatatat tattaaaaat ttttgccatt
      181 taaaaagaaa agcgacagtt taccaataaa tttcaactga attatctaaa agtagatcag
      241 ttagtttaga taattcagtt gaaattgacc gactcaaatt acttgtataa tacctaggaa
      301 aaaattaact agcaccacgg gtatagttat ttatatttat aacaaataaa ccagagaaat
      361 agcct
//
LOCUS       DAFCNJ010000213          360 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000213 DAFCNJ010000000
VERSION     DAFCNJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.224"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..358
                     /locus_tag="LNE35_003002"
     CDS             <1..358
                     /locus_tag="LNE35_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBK6912065.1"
                     /translation="FFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRRFPEGEE
                     VWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDDWQKVMA
                     AEVIATYLDGDLVYKK"
BASE COUNT          114 a           60 c           87 g           99 t
ORIGIN      
        1 cttctttaat tattgtcgat tagaggaaag tggtgtaaca gtcactagcg gtacagattt
       61 accgctgttt attactgata ttcctgaatc gattataaga gcagtattcc gtcgttttcc
      121 tgaaggagaa gaagtctggg agaaagaaac aagcttcacg acaaccgagc tgctgaaaag
      181 ttttacaagc aacagcttca aagtaaatgg tttatcggag tacggagaaa taaaagtagg
      241 gaaaagcgcc agcttagctg ttttcagcga ggacctctgt actgatgact ggcaaaaagt
      301 gatggctgcg gaagttatcg ctacctatct tgatggtgac ttagtttaca agaaataatg
//
LOCUS       DAFCNJ010000214          351 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000214 DAFCNJ010000000
VERSION     DAFCNJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.225"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            18..>351
                     /locus_tag="LNE35_003003"
     CDS             18..>351
                     /locus_tag="LNE35_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6912066.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRR"
BASE COUNT          120 a           71 c           71 g           89 t
ORIGIN      
        1 aaggagagtg aacataaatg acttataccc atcttacacc aaacgagctt gtaatgatag
       61 aagcatattt tcatcaagaa actccggttg ctatcgttgc gaagcagctt aaacgtggac
      121 gccaaacaat ttacaatgtc tataactttc tcaaatgtgg tggaacagca cttgaatact
      181 ttgaacaata caaagaaaat aagcgacgtt gtgggagaac cgaaatcatt tttcctgctg
      241 aggaaaaaga atacattgaa aaaagatcaa ctgaaggttg gaccccagac gtcattattg
      301 gccgtgcaga gcgaaccttc tcttgttcag taagtaccct ctatcgccgg t
//
LOCUS       DAFCNJ010000215          329 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000215 DAFCNJ010000000
VERSION     DAFCNJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.226"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>329
                     /locus_tag="LNE35_003004"
     CDS             <1..>329
                     /locus_tag="LNE35_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBK6912067.1"
                     /translation="YVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIVFT
                     MLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIWGI
                     ISKGWYM"
BASE COUNT           91 a           54 c           72 g          112 t
ORIGIN      
        1 gatatgttgg gtcgacgatt aatcccttca acgttttaat cgcacaggga gtagctggcg
       61 tttacggaaa tcccctgctt tggtttcgga tgctatcctg gattgtcatt actgggattt
      121 caattgtttt caccatgctt tatgctagca gagtcaaaaa gaatccactg tcctctattg
      181 tttatgagag tgatttaaag gttaagcata agtttcaaaa ttttgacact gaaaaagagt
      241 tttcaacaca agataaacta attatcgccg gttttatcgt aggtttagga atcatgattt
      301 ggggtatcat ttccaaggga tggtatatg
//
LOCUS       DAFCNJ010000216          321 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000216 DAFCNJ010000000
VERSION     DAFCNJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.227"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(69..321)
                     /locus_tag="LNE35_003005"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="LNE35_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAFCNJ010000217          308 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000217 DAFCNJ010000000
VERSION     DAFCNJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.228"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(76..>308)
                     /locus_tag="LNE35_003006"
     CDS             complement(76..>308)
                     /locus_tag="LNE35_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6912068.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAFCNJ010000218          305 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000218 DAFCNJ010000000
VERSION     DAFCNJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.229"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="LNE35_003007"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="LNE35_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBK6912069.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DAFCNJ010000219          301 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000219 DAFCNJ010000000
VERSION     DAFCNJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.230"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..301
                     /locus_tag="LNE35_003008"
                     /pseudo
     CDS             1..301
                     /locus_tag="LNE35_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           60 c           57 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 g
//
LOCUS       DAFCNJ010000220          299 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000220 DAFCNJ010000000
VERSION     DAFCNJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.231"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>299
                     /locus_tag="LNE35_003009"
     CDS             <1..>299
                     /locus_tag="LNE35_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6912070.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAFCNJ010000221          297 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000221 DAFCNJ010000000
VERSION     DAFCNJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.232"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>297
                     /locus_tag="LNE35_003010"
     rRNA            <1..>297
                     /locus_tag="LNE35_003010"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           92 a           61 c           76 g           68 t
ORIGIN      
        1 tcgggttacc gaattcagat aaactccgaa tgccattcat tcatatccgg gagtcagact
       61 gtgagtgata agatccatag tcgaaaggga aacagcccag accaccagct aaggtcccaa
      121 aatatatgtt aagtggaaaa ggatgtgggg ttgcacagac aactaggatg ttggcttaga
      181 agcagccacc atttaaagag tgcgtaatag ctcactagtc gagtgaccct gcgccgaaaa
      241 tgtaccgggg ctaaacatat taccgaagct gtggagtaca cctttaggtg tattggt
//
LOCUS       DAFCNJ010000222          282 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000222 DAFCNJ010000000
VERSION     DAFCNJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.233"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>282
                     /locus_tag="LNE35_003011"
     CDS             <1..>282
                     /locus_tag="LNE35_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBK6912071.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAFCNJ010000223          270 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000223 DAFCNJ010000000
VERSION     DAFCNJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.234"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>270
                     /locus_tag="LNE35_003012"
     CDS             <1..>270
                     /locus_tag="LNE35_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6912072.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DAFCNJ010000224          256 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000224 DAFCNJ010000000
VERSION     DAFCNJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.235"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           86 a           47 c           35 g           88 t
ORIGIN      
        1 gtatagcaag caaaattctt ctccacttcc agaatttttt attttcattt ttagaaaagt
       61 gaaaaaactg acaacaaaag cttgtttctt tcactctatt aatatcttta gtgaaaaagc
      121 tcgttttata tcgtattctt ccagcgaatg tacttctcat aagtccaaaa aaagctgcga
      181 aaaagaaagt acctcggtac ctactttttc gtagctgcac ataacttgag caagtattaa
      241 tcgaagatac ttttgt
//
LOCUS       DAFCNJ010000225          252 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000225 DAFCNJ010000000
VERSION     DAFCNJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.236"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(32..>252)
                     /locus_tag="LNE35_003013"
     CDS             complement(32..>252)
                     /locus_tag="LNE35_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK6912073.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DAFCNJ010000226          227 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000226 DAFCNJ010000000
VERSION     DAFCNJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.237"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>227)
                     /locus_tag="LNE35_003014"
     rRNA            complement(<1..>227)
                     /locus_tag="LNE35_003014"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DAFCNJ010000227          224 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000227 DAFCNJ010000000
VERSION     DAFCNJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.238"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>224
                     /locus_tag="LNE35_003015"
     CDS             <1..>224
                     /locus_tag="LNE35_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBK6912074.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAFCNJ010000228          220 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000228 DAFCNJ010000000
VERSION     DAFCNJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.239"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..188)
                     /locus_tag="LNE35_003016"
     CDS             complement(<1..188)
                     /locus_tag="LNE35_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6912075.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTK"
BASE COUNT           67 a           46 c           27 g           80 t
ORIGIN      
        1 aattttgtag tgtagctttt gccacctcca accaagcctt cccaaccatg atagttataa
       61 atccttaccc aatgtctaac cagtgtacga tttatttgat atttatgtgc aagatatttg
      121 tagccgcctt cgttatttaa atagtctgaa actacttttt tcttaaaaac aaatgtatat
      181 ttccgcaaaa aaagcacccc ttttaattag atttctagtc
//
LOCUS       DAFCNJ010000229          220 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000229 DAFCNJ010000000
VERSION     DAFCNJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.240"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>220
                     /locus_tag="LNE35_003017"
     CDS             <1..>220
                     /locus_tag="LNE35_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6912076.1"
                     /translation="METNGLSIQETAKKFNIGSNRTLSKWIEQYEEGGASSLESQKRG
                     RKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT           90 a           31 c           43 g           56 t
ORIGIN      
        1 atggaaacaa atggtctttc tatccaagaa actgctaaaa aatttaatat cggttcaaat
       61 agaactctaa gtaaatggat agagcaatat gaagaaggcg gtgcttcttc acttgagagc
      121 caaaaaaggg gcagaaaaat tagtatgaat tccaagctaa acattcctaa aaaacttaaa
      181 gatgagtctc ttgaagaaga agttattcgt ttaagagcag
//
LOCUS       DAFCNJ010000230          215 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000230 DAFCNJ010000000
VERSION     DAFCNJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.241"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>215)
                     /locus_tag="LNE35_003018"
     CDS             complement(<1..>215)
                     /locus_tag="LNE35_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6912077.1"
                     /translation="KQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFD
                     NAVAESTYKSLKVEFVYQYTFETLQQL"
BASE COUNT           68 a           37 c           35 g           75 t
ORIGIN      
        1 tccaattgtt gtaaggtttc aaatgtgtat tgatagacaa attctacttt caacgactta
       61 taagttgatt cagctacggc attatcaaaa ggacagcctt tatgactcaa tgatcgattg
      121 atgtcaaaag ttgttaataa ttcatcaata gcttggttat caaactcttt tccacgatca
      181 gtatgaaaaa tctcaacctc tgtcagaggt tgttt
//
LOCUS       DAFCNJ010000231          212 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000231 DAFCNJ010000000
VERSION     DAFCNJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.242"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           80 a           27 c           46 g           59 t
ORIGIN      
        1 aatcgatcag agtgaagcag acatacaaat tgaaagtcta caagaactgg aacaaattgt
       61 tttagaaaaa ggttagtgaa ttaatggaaa taaggctata tatctgtgct agctattttg
      121 actaaccaaa aaaacttgct ataattcagg tgtagtaaaa ggtgctggta acacctttta
      181 cgaaaatgct tcggcagtga taatgggatt gg
//
LOCUS       DAFCNJ010000232          211 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000232 DAFCNJ010000000
VERSION     DAFCNJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.243"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..>211)
                     /locus_tag="LNE35_003019"
     CDS             complement(36..>211)
                     /locus_tag="LNE35_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912078.1"
                     /translation="RIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIP
                     DLELRLLSDIVQG"
BASE COUNT           58 a           53 c           18 g           82 t
ORIGIN      
        1 tcaaaaatta ctcccattgt cattcgaatt cactcctatc cttgaacaat atcgctcaat
       61 aatcgcaact ctaaatctgg tatttcaatt gtgtattccc tttcaatctc aaaaaaagcg
      121 ctatgaattt tatccattat ttctccattt gcttgcatat attcttcaat gtcctcttgc
      181 tcatccacac tttctttacg tacaattcgt t
//
LOCUS       DAFCNJ010000233          206 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000233 DAFCNJ010000000
VERSION     DAFCNJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.denovo.244"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>206)
                     /locus_tag="LNE35_003020"
     CDS             complement(<1..>206)
                     /locus_tag="LNE35_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6912079.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DAFCNJ010000234        39259 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.guided.021, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000234 DAFCNJ010000000
VERSION     DAFCNJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.guided.021"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(177..884)
                     /gene="deoD"
                     /locus_tag="LNE35_003021"
     CDS             complement(177..884)
                     /gene="deoD"
                     /locus_tag="LNE35_003021"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK6912080.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(901..1719)
                     /locus_tag="LNE35_003022"
     CDS             complement(901..1719)
                     /locus_tag="LNE35_003022"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK6912081.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1743..2909)
                     /gene="deoB"
                     /locus_tag="LNE35_003023"
     CDS             complement(1743..2909)
                     /gene="deoB"
                     /locus_tag="LNE35_003023"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK6912082.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(3008..3964)
                     /locus_tag="LNE35_003024"
     CDS             complement(3008..3964)
                     /locus_tag="LNE35_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6912083.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3964..5097)
                     /locus_tag="LNE35_003025"
     CDS             complement(3964..5097)
                     /locus_tag="LNE35_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6912084.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5090..6652)
                     /locus_tag="LNE35_003026"
     CDS             complement(5090..6652)
                     /locus_tag="LNE35_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6912085.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6864..7946)
                     /locus_tag="LNE35_003027"
     CDS             complement(6864..7946)
                     /locus_tag="LNE35_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6912086.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(8012..8410)
                     /locus_tag="LNE35_003028"
     CDS             complement(8012..8410)
                     /locus_tag="LNE35_003028"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBK6912087.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8431..9093)
                     /gene="deoC"
                     /locus_tag="LNE35_003029"
     CDS             complement(8431..9093)
                     /gene="deoC"
                     /locus_tag="LNE35_003029"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBK6912088.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9139..10440)
                     /locus_tag="LNE35_003030"
     CDS             complement(9139..10440)
                     /locus_tag="LNE35_003030"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBK6912089.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10581..11597)
                     /locus_tag="LNE35_003031"
     CDS             complement(10581..11597)
                     /locus_tag="LNE35_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK6912090.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11905..12510)
                     /locus_tag="LNE35_003032"
     CDS             complement(11905..12510)
                     /locus_tag="LNE35_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6912091.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12706..13629
                     /locus_tag="LNE35_003033"
     CDS             12706..13629
                     /locus_tag="LNE35_003033"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBK6912092.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13688..15253
                     /gene="guaA"
                     /locus_tag="LNE35_003034"
     CDS             13688..15253
                     /gene="guaA"
                     /locus_tag="LNE35_003034"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBK6912093.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15321..16514)
                     /locus_tag="LNE35_003035"
     CDS             complement(15321..16514)
                     /locus_tag="LNE35_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6912094.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(16541..16741)
                     /locus_tag="LNE35_003036"
     CDS             complement(16541..16741)
                     /locus_tag="LNE35_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBK6912095.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            complement(17239..17469)
                     /locus_tag="LNE35_003037"
     CDS             complement(17239..17469)
                     /locus_tag="LNE35_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6912096.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            complement(17466..17888)
                     /locus_tag="LNE35_003038"
     CDS             complement(17466..17888)
                     /locus_tag="LNE35_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6912097.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            18419..18772
                     /locus_tag="LNE35_003039"
     CDS             18419..18772
                     /locus_tag="LNE35_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6912098.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            complement(18830..19018)
                     /locus_tag="LNE35_003040"
     CDS             complement(18830..19018)
                     /locus_tag="LNE35_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBK6912099.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(19116..20156)
                     /locus_tag="LNE35_003041"
     CDS             complement(19116..20156)
                     /locus_tag="LNE35_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK6912100.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20518..21015
                     /gene="dfrG"
                     /locus_tag="LNE35_003042"
     CDS             20518..21015
                     /gene="dfrG"
                     /locus_tag="LNE35_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBK6912101.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(21087..23039)
                     /locus_tag="LNE35_003043"
     CDS             complement(21087..23039)
                     /locus_tag="LNE35_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBK6912102.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(23046..23282)
                     /locus_tag="LNE35_003044"
     CDS             complement(23046..23282)
                     /locus_tag="LNE35_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912103.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(23517..24320)
                     /locus_tag="LNE35_003045"
     CDS             complement(23517..24320)
                     /locus_tag="LNE35_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK6912104.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(24336..24452)
                     /locus_tag="LNE35_003046"
     CDS             complement(24336..24452)
                     /locus_tag="LNE35_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK6912105.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(24697..25626)
                     /locus_tag="LNE35_003047"
     CDS             complement(24697..25626)
                     /locus_tag="LNE35_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6912106.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(25643..26665)
                     /locus_tag="LNE35_003048"
     CDS             complement(25643..26665)
                     /locus_tag="LNE35_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK6912107.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(26662..28689)
                     /locus_tag="LNE35_003049"
     CDS             complement(26662..28689)
                     /locus_tag="LNE35_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912108.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(28686..31139)
                     /locus_tag="LNE35_003050"
     CDS             complement(28686..31139)
                     /locus_tag="LNE35_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6912109.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            complement(31123..31518)
                     /locus_tag="LNE35_003051"
     CDS             complement(31123..31518)
                     /locus_tag="LNE35_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6912110.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            complement(31590..32228)
                     /locus_tag="LNE35_003052"
     CDS             complement(31590..32228)
                     /locus_tag="LNE35_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912111.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            complement(32284..32778)
                     /locus_tag="LNE35_003053"
     CDS             complement(32284..32778)
                     /locus_tag="LNE35_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK6912112.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            complement(32845..33624)
                     /locus_tag="LNE35_003054"
     CDS             complement(32845..33624)
                     /locus_tag="LNE35_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBK6912113.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            complement(33666..33887)
                     /locus_tag="LNE35_003055"
     CDS             complement(33666..33887)
                     /locus_tag="LNE35_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912114.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(33884..34174)
                     /locus_tag="LNE35_003056"
     CDS             complement(33884..34174)
                     /locus_tag="LNE35_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912115.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(34171..35355)
                     /locus_tag="LNE35_003057"
     CDS             complement(34171..35355)
                     /locus_tag="LNE35_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6912116.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(35537..36940)
                     /locus_tag="LNE35_003058"
     CDS             complement(35537..36940)
                     /locus_tag="LNE35_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK6912117.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(36962..37735)
                     /locus_tag="LNE35_003059"
     CDS             complement(36962..37735)
                     /locus_tag="LNE35_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912118.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(37745..38122)
                     /locus_tag="LNE35_003060"
     CDS             complement(37745..38122)
                     /locus_tag="LNE35_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6912119.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(38143..38457)
                     /locus_tag="LNE35_003061"
     CDS             complement(38143..38457)
                     /locus_tag="LNE35_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6912120.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(38657..39223)
                     /locus_tag="LNE35_003062"
                     /pseudo
     CDS             complement(38657..39223)
                     /locus_tag="LNE35_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
BASE COUNT        11453 a         8025 c         6778 g        13003 t
ORIGIN      
        1 tttttgaaat tgttccttgt gactcagagt taaacggcag attcacaacc tcttggtaaa
       61 cggtgttcaa aagcttacat ttgcaataat aagtccagcc aagtcaaaaa catgaggagc
      121 tatttttgct tggatgttct tattacttaa tgcagggcag ctttttcatt tccttcttat
      181 tctgctatcg ctgtttccaa tcctacttcg atcatttcat tgaacgtaga ttgacgttct
      241 gcagctgttg tttcttcacc ggtgatcaaa tggtcgctaa cggtcatcaa acttaatgtc
      301 tgcacatggt atttcgcgcc taagtaataa agagccgctg cttccatttc cactcctaaa
      361 acgccaagtt cagctaattg gaacgtctct gtcaaatcat ctttgtagaa agaatcttcc
      421 gaaagaacat ttcctacatg aaccgtatag cccttttctt tagctatctc atatgctttc
      481 agtaatagat tgaaatcact gattggagga aaatgaaagg cttggaaatt tttttcgatc
      541 atcgaagaac tggtcgctgc accttgtgct agtactaaat cacgaacatg gacgtctttt
      601 gacaatgcac cgcatgtacc tactcgaatc aatttcttta caccatacga ctggatcaat
      661 tcatgggcat agatggtggc tgatggcatt cccattcctg ttccttgaac agaaatacgt
      721 tctcctttat atttgccggt aaagcctaac ataccgcgta cctgattgta acaaactgga
      781 tcttccaaaa aagtctccgc aatatattta gctcgcaaag gatctcccgg aagtaaaatc
      841 ttatcggcga tctcgccttc tttcgcttca atatgcacac tcattttatt gttcctccta
      901 ttacaataat gtcaatgttt ctttaaccaa tgctttaaaa gattgtttga ctctctgagt
      961 tgtttctaca acttcttcat ggctcagatt cgcttgcata cctgctgcta agttggtaat
     1021 acaagagatt cctaacactt ttaatccgct gtgagctgct acgatgactt ctggaacagt
     1081 cgacatccct actgcatcgg ctcctattgt ccgagacatt cggatctccg caggcgtttc
     1141 ataagttggt ccagaatagc ccatatatac gccttcttta agatccaaac ctagtttttc
     1201 tgctgctgct tttgctgttg cttgatagtc aggcgtataa gcttgactca tatctggaaa
     1261 tctagggccc attgcatctt cattcggacc gatcaaagga ttttcaccag taaaattgat
     1321 atgatcggta atcagcatca aatctcctgg tacaaatgat tcgttgacac caccacatgc
     1381 attagtcaca atgactgaat gggcccctag tccggccatc acacgtactg gataggtcac
     1441 tgtttgcatg gaatgacctt catagtagtg gaatctccct tgcatggcta acactttctt
     1501 gcctgccaga gacccataaa ctagctttcc tgcatgccca actactgtcg aaacaggaaa
     1561 gaacgggatc tcatcataag aaatcattac agcatcttcg atttcatctg ctaattcacc
     1621 taatcctgaa cctagaatca aaccaaaatc aatttctttt actcctttat caaggataaa
     1681 ctgggtcgtt tcttgtaaca tctcgcttaa cttggtcatt tgctttctcc tatttaaaaa
     1741 gattatttta ataatttcaa gaagctttcg ccattttcag tcgaagaaac actaaagttc
     1801 tcagcaatcg ttgctgaaat atctgaataa tatccttgag gcaatgcccc ttgttctttc
     1861 atttttttac tgtaaaccaa taacggtaca tattctcgcg tatgatcggt accagtaaac
     1921 gtcggatcgt ttccgtgatc ggctgtaatc attagcaagt cgtcttcttc catcgcttca
     1981 agcaattctg gaattcgtaa gtcaaattct tcaatcgcat gcgcatagcc gactacatcc
     2041 cgacgatgac cgtataatgc atcaaaatct actaaattgg taaagctgat tcctgaaaaa
     2101 tcgtttttca taactttcag cagctggtca acgccatcca tattgctttt cgtgcggaca
     2161 gattcagtga ttccttgtcc gttgaaaata tcgttgatct ttccaacagc aatcacgtcc
     2221 tttcctgctt ctttcaaaga atcaaggact gtgtgtccga atggattcaa cgcataatca
     2281 tggcgattgc ttgttcgtgt gaagtctcct ggttcaccca cgtacggacg tgcaatgatt
     2341 cggccaatca tatacggatc atcttttgtt atatcacgaa cgaattggca aatccgatat
     2401 aattcttcta aaggaatgat ttcttcatgt gccgcgatct gaaggacagg atcggcagaa
     2461 gtatatacaa ttaaatcgcc tgttttcatt tggtgttcac cgtaatcgtc aataacagct
     2521 gttccgctat aaggtttgtt acatacgact ttacggccag aaaattcttc gatttgtttg
     2581 atcagatcat cagggaatcc attaggaaac acacgaaatg gagttttgat attcaatccc
     2641 atgatttccc agtggcctgt catcgtatct ttccctacag aaacttcttc taatttcgtc
     2701 gcatagcctt gatgatcttt cactgattcc acaccttcaa gaggacggat cgttcctaaa
     2761 ccaagctgct ctaaatgtgg aacgttcaat cctgcttctt tcgcaatgtg tcctaatgta
     2821 tcagaaccaa catcaccgaa cttatcggca tctggtgctt ctccaattcc tactgaatcc
     2881 atgacgatca gatgtacgcg tttaaacatg attattcctt ctttcttttt atttttattg
     2941 cctttaaaac aaaaagggcg agacgaaaaa cacttcccgt aacccaccct ttttattcgc
     3001 taaaagatta ttttgatttg atatagttct tcccgtttgc ttttggacca gaagctttcc
     3061 ctaagaaaac aaccagcaca atgattgtca gcaaatatgg agcaatttgc agcaatactg
     3121 gcggaatcga agaaataact ggtaattgtg cgccaatgat actcaagctt tgagcaaaac
     3181 caaagaacaa tgaggcaccc attgccccta aaggattcca cttaccaaag atcatagcag
     3241 ccatggagat gaacccttgt ccaacgatcg taccagctga aaagttaccg gaaatcgact
     3301 gagcaaaaac agcaccgccc ataccgccta acaagccaga aatcagtacg cctgaataac
     3361 gcatagcata tacattgata cctaatgtgt cggcagcttg aggattttca ccaactgaac
     3421 ggaggcgcaa gccgaatttc gtcttgaaga tgatcaccca agctaggaca gcaatcaaaa
     3481 tagcacaata agcaggtaag aatgtatttt taaagaaaat tggtccaatg accggaatat
     3541 ctgctaatcc tgggaaagaa aaatacccca gattctgcgt gatgttatca gtttgacctt
     3601 taccatacca tgctttgatc aagaaaacac ctaaagcagg tgccatcaga ttaattactg
     3661 tcccgctgat aatatggtca gcacgcaaac tgactgttgc tactgcgtga atcagagaaa
     3721 ataggatacc tacgacgcca cccactaaaa tagcgatcca tggtgtcata gaaccaagtg
     3781 atggactcat cgtcaagttg aaaacgatgg aactaaaagc acccatgacc atgattccct
     3841 ctagccctac gtttacgatc ccgcttcgtt cagaaaaagt tccgcctagt gacgttagaa
     3901 ttagcggtgt tgaataaacc agtgtagaag taaggatcaa ggcaattaat tcgatagtcg
     3961 acattatagt tcgccccctt catggttttg attcgttggt ttcgattcta ttgtcgcaac
     4021 gatctcttct tgttttttcc cttggaagac tttagccatg acgaaacgaa tcaggaagtt
     4081 gatcccgaca aagaagataa tcaaagcaat cgatacattg acgatctcaa acggaacacc
     4141 tgctcctgtc tgcatgccaa gtccaccgat cttcaaaata ctgaacaaga tcgctgataa
     4201 aagaataccg attgctgatc cagaaccaag caaggaaaca gccattccgt caaatccaat
     4261 agatagcgaa gtcgtttgaa caaagaagtt ttgatacgtt cctaatcctt caaccacacc
     4321 gccaagccct gccaaagcgc cagaaatgac catcgacaaa acgatcgtac gtttactact
     4381 cattcctgca tactcagaag caaaaggatt caatccaaca gaacggattt caaatcctaa
     4441 agtcgttttc ttcatcagca gccaaacgaa aataagcgcg atgattgcta aaaataatcc
     4501 tgagtttacg cgagagccgc caaaagcttg tgttaaccat ggtaaccgca gtgaggcatt
     4561 ttgactgatc atattggaag agtccaaact tgagcgatag ctttcaggca tcacttcatg
     4621 taaagcaaat gtgcttacgt ataataagat atagttcatc atgattgtta cgatcacttc
     4681 acttgttcca aaaaatgctc gaagtaaacc tggaacagcc gctgccatcg caccagctaa
     4741 ggcacctaag atcacgcata acggaagcaa aacaactttt ggcagatctg gaaacgcaag
     4801 tgccacccag acactgacga tccacccgca aagtgcttgt ccagaaagcc caatattgaa
     4861 aaacccagct gagttagcta cagaaaaacc taatgctgta aaaatcaacg gcgttgcttg
     4921 ccttaacgtt tcacctatcc ctctttgcga accaagggct gcatctagca tagaagaata
     4981 accttggatg ggctgaaaac caaaagcagc catcaatatc gcgcctaaga tcaatcctaa
     5041 aacaactgaa agtacaggca ctaataaatt acggaatttt tcgctgcgat cattcatgtg
     5101 cgccagcccc cttttctaac tcttttcgtg cttcttctaa ggtatagccc gccatcaata
     5161 gacctaattc attttcagat gtttctttcg gatcaacgat accgacaatt tctccttcat
     5221 ggataacagc aatacgatcc gaaacattta aaatttcttc taattcgaaa ctgatcagta
     5281 acactgcttt gtatttgtcc ctttgttcga tcagacgttt atgaataaat tcgatcgcac
     5341 ctacatccag tccacgagtt ggatttgcaa caatcaacag atcaggatct cggtcgactt
     5401 cacgagcgat aattgctttt tgctgatttc cgcctgataa agcttttgcg ggaacaagtt
     5461 cattcgttgt tcgaacatca tattcttcga tcaattctct ggcatgttca ttcattactg
     5521 aataattcag catgccgttt ttactgtaag gtttttgatg ataggtttgt agtgcgatat
     5581 tttcagataa tgtcatatct aggactaacc cgtatttatg acggtcttct ggcacgtgtc
     5641 ctacaccatg ttcagtgatt tttcggggtt ttttattagt gatgtcttcc ccttttagct
     5701 tgatatgtcc gctttctgcc tttcgcaaac cagtcaaagc ttggatcaac tccgactgcc
     5761 cgtttccatc gattccagcg ataccaagta cttcgccagc acgaacctct aagttcaagt
     5821 ttttcacggc ttctaatcca cgattttctt tcaccactag attttcaata gacaaaacga
     5881 cttcttgagg cttcgcttct tttttcatcg ttttgaatga aaccgttctt ccaaccatca
     5941 tatcggctaa ttgttgtgag gttacgtctt taacgttgac tgtaccgatt ccttttccac
     6001 ggcggataac tgtacagcgg tcagctactg ctttgatttc atctaactta tgcgtaatca
     6061 aaatgatcga cttgccttct ttgactaatt ccttcatgat cacgattaat tcatcaattt
     6121 cttgaggggt caatacagct gtcggctcat caaaaatcag tacatctgct cctcggtaaa
     6181 gtgtttttaa aatttctacc cgttgttcca ttccaactga aatatcacga acgtaagcat
     6241 ctgggttgac tgacaatcca tactgttcag aaactttttg gatctctttt cgtgcttttt
     6301 tatggtcaag catccctgcg cgactaggct cgcttcccaa cacgatgttt tctgttacag
     6361 taaaggcatc aacaagcata aagtgctgat ggaccatccc aatccctaat tggttggctt
     6421 tggtcggact tgtgatcttt acttctttcc cacgcatcaa aattttccct gatgtcggct
     6481 ctaataatcc ggaaagcacg ttcatcaatg tagatttgcc agcaccgttt tctccaagca
     6541 acgcatgaat ttctcctgcc tttacttgta aattgatgtt gtcatttgcc ttgaatgttc
     6601 caaattcttt ggtaatattc tgcatttcta tgacaatatt ttcttgaacc accttttttt
     6661 cacatcctaa cgatttctat ctagggcaac gattgactaa gattcacgct tagtgagcgt
     6721 tcttcttttg aaacaaacat tacttcaggg agttgttccc ttatccaaac tgtcacaagg
     6781 cagcttggat aaatgaacaa ttcttgttct aatcaaacca tagccttgtc aaacatgtgt
     6841 ctaacaaggc tatagaaatg gttttagttt tcttcaggag tttctggaac ttttacgtca
     6901 cctgaaatga ttttttcttt tgcttcatca actgcttttt tcgcttcgtc agaaagttgt
     6961 ccttctgtta gaccaacacc gtcttctttc aatccataaa cagtgtgttc gccgccaggg
     7021 aatttaccgt ccgctgattt ttgtgccaaa tcttctacta ctgttccaac tgctttcagt
     7081 gtagatgtca atgtgaagtt tttcttttcg ccgtttaatg tgtattcgcc ttcgtctgat
     7141 tggtcgcggt caacaccgat aacccaaact tttttgtcgc ctgattcgtt caatgatttt
     7201 gcttcttgga atacaccgtt acctgtaccg cctgaagcat ggaagatgat atctgcgttt
     7261 tgtgcataca taccttgagc aattgaacgt cctttatctg gagcagagaa gtcaccagca
     7321 tattgattca atactttgat ttcttttcct aattctttcg cacctgcatc aacaccggct
     7381 ttgaatccag catcaaaacg atcgattact tcacctttta caccgccgat aaagccgact
     7441 acgtttgttt cagtcgtata agctgctgca acacctgcaa gataagaagc ttcgttgtct
     7501 ttgaaagttg cagatacaac gttatctaat ccatcgatga catcatcgat aataacaaag
     7561 ttcgtacctg tattgtttgt tgcttgttct tggatcgctg gttttagttt ataaccaata
     7621 ccaaaaatcg ttttgaatcc agcattcaat gcttggtcaa tatttgggat gtaatctgat
     7681 tcatttgaag attggaaata ttggaagcca tcatttcctc gtgaaagtcc ttgatctttc
     7741 ccccattttt ctagtccttc ccaagctgac tggttgaatg aacggtcgtc tacaccacct
     7801 gtgtctgtga tcaatgctgc agttgttgtg tctgatgagc ttttgccacc ggatgaatca
     7861 gtagagccgt tgttgccgca tgcacctaaa aataaccctg ctgctagtgc gacagcacct
     7921 aaaccaaata attttgcttt tttcatttct gtaaagcctc ctaaaataat tgttttattt
     7981 ttcaacagtc agctgactga atgaacatga gttatagatc tttttcagta aatgcataag
     8041 gcagcagtgc acgaacggtc gttgccttcg tcacaccatt gtctcctact agcgtaaccg
     8101 gcatatcagg ggaacagaat tctgccatta cttgtcgaca tgcaccacaa ggagagatag
     8161 gatcgggggt gtgccccgct acgactagat gcgtaaatgt tttttctcct tcagatactg
     8221 ctttgaaaaa agctgtgcgc tcggcacaat tcgttaatcc aaatgatgca ttctcaatgt
     8281 tgatgccttg ataaatcttg cctgattccg ttacgagaca agcgcctacc ggaaaatggg
     8341 aataaggaac ataggcttta tctaacgctt caatagctgt gttgatccat tcttgtttga
     8401 gttctgccat gagcttctcc tttgaaaaaa ttaatagcct tcaccagttg aaccagatac
     8461 gattgctact cctgcgcttg ttccgattct tgtagcacca gcttcgatca ttgccattgc
     8521 ttcttcttcg ttatggatac cgccggaagc tttgacaccc atttctggtc ctacagtttc
     8581 acgcatcaaa cggacatcct ctacctttgc tccgcctgtt gagaaaccag ttgatgtttt
     8641 gacaaaatca gcgcctgcag cttttgctat ttcgcaagct cggatgattt cttctctatc
     8701 taataacgat gtttcaataa taactttgac aagcgcacga tcttttgctg cttcaacaac
     8761 tgcttcgata tctttttgga cttgttgatc atcctttgat ttcaaagcac cgatattgat
     8821 gaccatatcg acttcatctg caccgttatt gatggcatct gttgtttcaa atgcttttgt
     8881 ctcgggtgtg tttgctccta gaggaaaccc gattactgta catacggcta ctggctcacc
     8941 ttgtaattgt tctttcgcta gcgcaaccca tgctggattg atgcagacag aataaaaatg
     9001 gtattttttt gcttcttcga taatttgcat cacagcttct ttagatgcgt ctgctttcaa
     9061 aatagtatgg tcgatcattc ggtttagttc catttctttt ccccttttct ttaaaacggt
     9121 aaagtccgct tttttccttt attctgtgat gatgtcatga atcaacgttg gcttcattgc
     9181 cgattcacca atttcaatat ttttatataa taattcaatg acagaagtga tttcttcgtc
     9241 gttactatag atcgtcaata gtgactcacc ttttgtcaca gtgtcaccaa tttttttatg
     9301 cagtttcaat ccaacagcgt gatcaatatc ttcttctttg gttttacgcc cagcacctag
     9361 catcattgca gcaattccta attcatttgc cacgattttt gacacaactc cgctttgttt
     9421 agcaggaagc tccattgtgt agcgtgcagt caaaattcgt tctggatgtt ccacgatcgc
     9481 tgggtctccc ccttgatttt ggatcatttc tttgaatttt gctaacgctt tacctgattc
     9541 cagagcttct tgaagcaacg ttcttgcttc atcggttgtc ttagctttgc cagcaagaac
     9601 aaccatttgg cttcctaagg cgtagcacat ttctactaga tcttctggac catttccttg
     9661 taatgtttcg attgcctcga caacttctag actatttccg atagcttcgc caagaggttc
     9721 tgacatatct gagataaccg ccactgtttc tcggttagcc agtttcccaa tgctcgtcat
     9781 ggtctttgcc agacgtcttg catcttcgat gttcttcatg aaagcaccgt ctccagtggt
     9841 cacatccaaa acgattgcat ctgctcctgc cgcgattttt ttactcataa tcgaactggc
     9901 aatcagcgga agagaatcaa cggtcgctgt gacgtcacgc aaggcataca attttttgtc
     9961 cgccggtgcc aagtttcctg attgaccaat taccgctact ttactttcat taacaatgcg
    10021 tacaaattct tcatccgaaa gctcgatctg aaatccggga attgcttcta gcttgtctag
    10081 tgtaccacct gtgtaaccta gaccgcgtcc agacatttta gccactgtca ctccgacact
    10141 agctactagg ggggctaaaa tcaatgtggt cgtgtcacca acgccacctg tcgaatgctt
    10201 atctaccttt attccttcaa gagaactcaa atcgatgaca tcaccagaat tagcaatcgc
    10261 caatgttaag ttcgtgattt cttcatttgt catgtcttga taaaaaattg ccatcaataa
    10321 tgcactgact tgataatctg gaatctttcc attcgtatag ttcgtgacga tgtactcgat
    10381 ttcctctttc gacagctcgt ttccatccct ctttttttca atcagatcca ccattcgcat
    10441 tgcctgatcc tccttgtctt cattcggtat tatacattat ttttaggaaa ggtaaaccac
    10501 tttagtaaac tggtttacta aaaataaaaa aagcactaac tagcctttgt aaatgcttac
    10561 ataagcaata taccgccgag ttagcccttt gtcaatactt ttacgctctc acgaataatt
    10621 aattttgttt cgaacacttt gttcggaact tttctttgag gaaattcgat cgtatcgatc
    10681 aggaaacgtg cagcataaaa tccaatatca aaacttggct gctgcactgt cgtcaattgc
    10741 ggaatcatgt attctgacag ctttaatcca tcgaatccta tgactgagat atcttctggg
    10801 atttttttgc ccaattcatc gatcgcctga tagcatccga tcgccatggc atcattcccg
    10861 caaaaaatag ccgtgatatc tttatgtttg agtaattttt ttgatgcagt atatccacct
    10921 tggacagtca actctccatg ttcaacaaat ttcccattaa aaggcaggcc atgatctttc
    10981 agcgcatgac gatatccgtt gaaacgttct tctaattgat agtatccagt ggtttccttc
    11041 agcatgccga tcttcgtatg gccattttca ataaacagcg aaacagcttg ataggcgccc
    11101 tcatattcct ttacgataag ccgtccgtta tcccgacgat tcaatccgcg atcgattaaa
    11161 atgattggca ttttttgata aaaaccgctg tctaatgcat atttctcagg taaaatattt
    11221 ggtgtggcca gcaagattcc gtccactgag cggtgggaaa gctcttgtaa atacttgatc
    11281 tcattttctt ggctatgacg agaattgcat aacatgatca catagcccaa aggattcaaa
    11341 tagctctcta caccttcgat cagtttggag aagaagaaat cagtgacatc agggacgatc
    11401 attccgatgg tctttgaatg tcgattgatc acatttgaag cgaaataatc gggcttataa
    11461 tgatgttcat tcacgatcga caacactttt tttcttgtct tatcactaaa tctgcttcct
    11521 ttgttattca aaatctgtga gacagttgtt actgatacgc ctgccatttc tgcgatatct
    11581 ctgatggtca gcttcatcct attggctccc ttctatctaa attccctatc agtgtaccaa
    11641 aacccccaag aaaagaatag agaaaaaagg aaagtatgtt ttttgataaa aacctccttt
    11701 atctaccgac ttggcagcct tctatacaag ttctgtatct tatttatttt atcgtaaaaa
    11761 acaacgactc tgttcctact atttcttttt gaagaaaaaa tagttctttg tttttaaaaa
    11821 cttctcaaca aaaaaagaac tgatacgtac ccgtacatgc acgcccagtt ccactctttc
    11881 gtcgctccac tatttttcag ctagttattc tttcacactt ttgatgatat aatatccttt
    11941 atcttttgtg acgatttctg cattaccaaa tgtttcttcc attttctttt gcgcactcgg
    12001 cgctccttgt ttcttctgga tcacaattgt cagtgtaccg cccttcttca accgaggata
    12061 tgcgcctgtc aaaatttcat gtacaacttt ttttccagca cggatcggcg gattgcttac
    12121 gatagctgca taggtttctt catgaactgc ttcatagata ttcgattgat ggatatcaac
    12181 atgttcgatt tggttacgct ttgcgtttcc ttgcgctaga cttaccgctc gttcattaat
    12241 gtcgaccatt tcaacaaacc gctgtgtcgc aaatgccaaa gctaacccaa tcggtccata
    12301 cccgcatccc acatctaaga tctttccttc atcaggtaac tcttcccaat cgaaagcatc
    12361 aatcaggaca cgtgaaccat aatccactgt ttcacgtgaa aatacaccag aatctgttac
    12421 aaattgaaag gttctgcctt ttaactcaaa agcccattgt tcaaaatcgt gcgccgtatc
    12481 tgggcgttca gaatagtaat gatttgtcat gtcttccatc cttctcattt ctttcatgac
    12541 aacagtctat ccttttatgt atccgtgcgc aagtcaagaa taagactccg tttctttcta
    12601 ttataaagat tatacaaaaa gatcggcaat ctctcttaag tggtggggtt tgcgtttatt
    12661 ttgtgttaaa atagttccat tgatgggaga acgcaggagg ctaatatgaa tgatccaatg
    12721 aactactacc gtgtggcaag agaagaatgg cgagaatttt atcgtaatgg gcaagcacct
    12781 ttgacacaag atgaactaga taatatcaaa agtttgaatg accgcatttc catgcaggat
    12841 gttcgagata tctatgtacc tcttgctcat ctgatttatc tttatatgaa agaatttgaa
    12901 tctttgttct tgagtaaagg tttatttctt cataaatacg tagaagtacc gccttttatt
    12961 atcgggatcg ccggaagtgt agccgttgga aaaagtacaa cagccagact gctgcagacg
    13021 attttgtctc gtaccttcaa acgaagaaac gttcagctga tcacgactga cggatttctt
    13081 tatccaaatc gtatattaaa agagcgtgga atcatggacc gtaaaggatt tcctgaaagt
    13141 tatgatatgg agatgctgat cgatttcttg aaccgtgtaa aatctggtca ggaagagata
    13201 aaagtacctg tttactcaca tgatgtgtat gatatcattc cagacaagta cgaattgatc
    13261 cagcagcctg atatcttgat cgttgagggg atcaatacgc ttcaattacc tgcgaatcag
    13321 caaatctacg tcagcgactt ttttgatttt tctgtctatg tcgatgccga tcccgtgctg
    13381 atcgaaaaat ggtatttgga tcgttttggt gcgttactgg atacggcttt ccaaaatcca
    13441 gagaattatt attatcgttt tgctatcgga gatcgtgaag aagcctttaa aatggcaaaa
    13501 gatgtatgga aaaatgtgaa tttgaaaaat ttacaggaat atatcttgcc tacacgagga
    13561 agagccgata taattctcca taaaactgaa aatcatatca tcgattcgat tttcatgcga
    13621 aaatattagc attttaaaat agtttgacta ttttgagata aaatttaaag ggataggggt
    13681 aataaatttg acaaacgttg ccgatatgac gaacgttgaa aagattatcg tattagacta
    13741 cggtagtcag tataaccaat tgatcacaag acgtattcgt gatcttggag tcttttccga
    13801 attattgagc caccgcatta ctgcagaaga agtaaaagcg atgaatccaa agggaatcat
    13861 tctttctggc ggaccaaaca gtgtatatga tgagaatgca tttgggatcg atcctgagat
    13921 cttcaattta ggtattcctg tattagggat ttgctatggt atgcaattga tcacgtacaa
    13981 acttggcggt tccgtagagc ctgctaaaaa ccgcgaatat gggaaagcct tgttagaagt
    14041 aacaagcgat caagctgtct tgtttaaaga aacaccaaaa caacaaacag tatggatgag
    14101 ccatggtgac ttggtaacta aagttcctga aggctttgaa acagtcggaa caagcaaaga
    14161 ttgtccgatt gcttctatcc aagacacaac acgtaatttt tatggtatcc aattccatgc
    14221 agaagtacgt cattccgaat acggaatcga attgctgcgc cactttgcct ttgatgtttg
    14281 tcactgtgaa ggtaactgga caatggataa ctttatcgat atgcaagtag aaaaaatccg
    14341 tgaacaagtt ggtgacaaaa aagtcttgct tggtctttca ggtggcgttg attctagtgt
    14401 cgttggggtt cttttacaaa aagcgatcgg tgaccaattg acatctatct ttgttgacca
    14461 cggtctgcta cgcaaaggcg aagcggaaca agtaatggaa agtctaggcg gaaaattcgg
    14521 cttgaacatc atcaaagtcg atgcaagaga tcgcttctta gacaagttga aaggtgtatc
    14581 tgacccagaa caaaaacgta aaatcattgg gaatgaattc gtatatgttt ttgatgacga
    14641 agcaacaaaa cttgctggtg aagaaggcgt ctctttccta gcacaaggaa cgttgtatac
    14701 tgacgttatc gaatctggta cagaaacagc acagacgatc aaatctcacc ataatgtagg
    14761 tggactgcca gaagatatgc aatttgaatt gatcgaacca ttaaacacat tattcaaaga
    14821 cgaagtccgt gaattaggga ctcagttagg catgcctgat tcaatcgttt ggcgtcagcc
    14881 attccctggt cctggtcttg gtattcgtgt tcttggcgaa atcactgaag acaagctaga
    14941 aatcgtacgc gaatctgatg ctatcttgcg tgaagaaatc gcagctgctg gacttgaccg
    15001 tgatatctgg caatatttca ctgttcttcc agggatccgc tctgtcggtg ttatgggtga
    15061 cggacgtact tacgactaca cagtcggtat ccgtgctgta acttcaatcg acggaatgac
    15121 cgctgacttt gcccgtatcc catgggaagt cttgcaaaaa atctctgtac gtatcgtaaa
    15181 cgaagtagcc cacgtcaacc gtatcgtgta tgacatcact tctaaaccac cagcaacagt
    15241 ggagtgggaa tagttgtagg agagctagaa atcctcttat accaagggtt tctggctctt
    15301 cttatctttt tactgcactt ttattgcact tttttcattt ctttgtttcg caagtagtct
    15361 acagtaaatt gattctttgg tgaagtatca taattttgtt ttgcttcttt aaaggaaatg
    15421 gctccattta atttgttagc tacctcgaaa ttactgttag gataaagatg tccatacgtt
    15481 cctaaagtcg tctcaatatc ttcgtgtcct agacgatctt taatgataag tggattttct
    15541 cccatactga ttaacaagga agcatgagaa tgccgtaacc catggattct gattcgatgg
    15601 ataccagcca gcttagcata gcgttcaata gcatatgata gtgtatgttt ttgggtagga
    15661 ataccgttat agctcattac aaagtctgtc tgaattagat tttgttgtac ttccttccat
    15721 tcggataaat aagttaatgt gcatttatct aatacaatat gacgaacgct cgcctttgtt
    15781 tttggttcaa caaatctata attcgattga ttcttgtaat agagtgtttt gttgatggtt
    15841 agtactcctg agtcaaaatc aatatcttcc cattgaatgg cagtagcttc accaattcgc
    15901 attccagtca taaataaaaa ccacagagag ataaataaaa agtgttgata gtaatcttct
    15961 ttgtaaatta gagagattac tttttcaaat tcttcttttg tccaaaattc aattttagac
    16021 ttttgctttt tcacattgcc aataatctta gatggatttg tggtggttat cccaaggaca
    16081 attgctcgat ccatagcaac tgaaaatagt ccttgaacag ctcttacata agaagactta
    16141 cacttctttg ataattttag ttgccaattt tgcacatcaa taggctcaat atcggaaaca
    16201 gccattttat caaaataaga gaaatgcttc ttaatagttg gtaagcgatt ttcatatgtt
    16261 ctgagcttta cctgtgtctt ataccatgga aggaaaattt caaagatata atgtttaaac
    16321 gtcattttat ccttattctc acttaattct tctggtttca tcaatagcag tttcgaatat
    16381 tcttctcttg cttctttttt agtagtgaat ccactacgat atttttgaat ttttttccct
    16441 ttagaatcat aacctagatt tgcacgaaaa taataggttc cattctttgc tttttttatt
    16501 gggtctttaa ccatagtttt tactccttac tgtgcaaaaa ctactcagac ctgttttcat
    16561 ttgaaaaccg aattcctaga atttcttcaa cagcttcacg agggactcta tctagctttc
    16621 gagattgata gtagatatgc ccttttgtaa tcatcaattc ttttgctttt tttatgatgt
    16681 ctgctgaaaa agaagttcca tatcctagct caactaaatc cttttttgtg accgttatca
    16741 ttattactcc tttcccaatt ataggctagg aaaactccaa tattcaccta taattatatc
    16801 aaatagtttt ataataatac actgacaaga gaagaatatc ttagcaatcg aacagctcta
    16861 gcaaagctca caggaatttc acttctgcat ggttctcatg tagccgtacc cattgcctgt
    16921 gacgcttcta acgctcggac tatggctata cgggagtatc attatcacga taagaaagtc
    16981 atggcagcaa tcctgctaaa gattactttc gaattactaa catgaatcgc tcactaattt
    17041 tgtaggtcat ggcgtgttag ttcatcagct cttttcttac cgaaacgtat tcgattgcct
    17101 ttatttggtt gtcaaagaac gtttggcttg ttagcctatc aaaacctact gaacacacaa
    17161 tatgctttga tggaataaca aacctcccaa atcgggaagc gtggatacga ttcgacacgc
    17221 ttccacgaaa aaatgggact atttaatttc gaacgataaa atcttagaga tgagtttcgt
    17281 ttccattctg ccacgtaggg tttcatcaat aaccatatga gaatttccat attcgtctgt
    17341 catgcgacgt aatgaacgtt ttgaggtata ccctcgataa tgtttaacaa tctgattaat
    17401 tgcttccacg tcgccatctg tcgcctttac aatgaggggg aagggaaccc ttggataagc
    17461 tgttttcatt ctttacactc cttcatgaat tctttaatca tggctagtcc acttattcga
    17521 tgacgataaa cagtcgaacg attcaaattt agtaactccg caatctccac atcactcata
    17581 tccataaagt aatgaagtaa aataatgttc cgttttttct ctgaaagatg acgcagggct
    17641 tcacttaata aatcactttc aacattgatt cttaatccaa ataatttaaa tagttggaaa
    17701 tcagtgacgt acgtatcaac agtagaaaaa gagttgacaa ggtagttatc tatatcagaa
    17761 aacgagactt cttttgctga tagtctgtct agatgtttga agtaatcttt tcgttcatct
    17821 tcaataactt gtttgcagat atagtcaaac tgattttcta tggtttcttg aaaagaagat
    17881 ggtttcatgc ttttcacccc ctttctgtcg tgaaagggag tggcatgtgc ttctttatct
    17941 cccttcatac taaaccccga cacgaacggg ggatttgttg cataaatgaa gagaaatcct
    18001 aaaagatatt tattaagtga ccaaaaaagc acataaacag attgttttct ctgttcatat
    18061 gctttgattt ttctatctat gtaatctgca aaaccacatt gatggtcata tctatccagt
    18121 acaggtggat aagttttttt gataagaact taattaagag aactagatga cagattatct
    18181 ttctcatggc gacttttccc tccattaagt cgccaatttt atctactttc taggagtatg
    18241 actaaatttt ataaagttgt ataggtaaat aaaatcagcg acctttctat ttgaaattgg
    18301 attcatcgca ttaataaatt ccacagtgtt ctataggaac tcataaacta tataacatgt
    18361 ttttctatac ctgtttatgg ttatatagga acagtaaaat gtatagaggt gatttagtat
    18421 gcgtaaaaaa gaagataaat acgattttag agccgttggt ttagcgatta aagaggctcg
    18481 aatgaaacgg ggtctcactc gtgaacaagt gggaacaatg attgaaattg acccacgtta
    18541 tttaacaaat atagaaaaca aaggacaaca cccaagtaca caagtgctgt atgatcttgt
    18601 atcattactc catgtatcta ttgatgaatt tttcttacct acagataatt tgattaaaag
    18661 cactcgaagg ttacaggtag aaaaatacat ggatagcttt acagacaaag aactatcctt
    18721 aatggaagca ctagcaaaag gtatcaatga agcaagaaat attgaaggct agtcagtaaa
    18781 attcagataa acaaaaagag ccgataagat gagagtttca tttctcaaat tatcggctct
    18841 gcgtcttggc gtctggctct ttgattgtta ttatattcat tttttgaaca ttaattaaag
    18901 tttcgttctt acatttgggg cagtataaag ggaaattttt aagtatagta tccactcgta
    18961 ttcgtagtct tgttttattt ccacaaatag gacacaatat ccacttgtag tttataataa
    19021 caatctcctc ctttccactt taattcaaat ctatattaaa gaatatttca tcttatttaa
    19081 taagaaacca tatttatata acaacataaa acgcactaag ttattttatt gaacatatat
    19141 cttactttat ctatccgact atttagacga cgggtctggc aaacaggttc gccagtggta
    19201 acctgatatc cttttagctc tgctaaacaa acactaagcc catttgtaaa aaaagttaaa
    19261 tcattgcgat aatcttgaat acatcgagca ggaatttctc caataataat gacctcatta
    19321 tttttcagtt gagtatttac gatatttgca caatatttgg gagcatcgtt atatgcccgt
    19381 gaaagatatt cctgtggtgc ataaacttta aaactaagat atggctctaa caattctgtt
    19441 ccagcttttc taaaggcttg ctccagtaca ataggagtaa gcatccgaaa atctgctgga
    19501 gtactaacag ggctatagta taaaccgtac ttaaaacaga ttttacaatc cgtcacattc
    19561 caaccatata atccttgttc gcaaccatag cgtatccctt ccataactgc attttgaaat
    19621 gattgattta agtatccaag agaaaccgag ctctcatact gcattccact tcccaacgga
    19681 agcggtgata cagataaacc aatggaagcc cagaaaggat ttggcggcac ttcgatgtga
    19741 atggtatatt ctgcattttt taacggtctc tccatataaa tgactgtagg ctcttttagt
    19801 tctatctcca catgatactt ttcttgcaac agtgcactaa tcacttccat ttgtactttc
    19861 cctaagaaag aaagtataat ttcatgtgtc gtagaatcca cgtaatatcg tagaagcgga
    19921 tcactatctg agatttccaa aagggcatca agcaacattt ctctctgttc aggtttactc
    19981 ggttcaacag ttgtttgtag tagagggtgc ggattttcaa tcttttttct ctgtggcaat
    20041 agttttgtat ctccaagaac actatttaac ttcaaaaact cattttgcaa aataacaatt
    20101 tctccagaat aagctctatc aatcttacat aattcaccat ttattgaagt atacatttct
    20161 gtaactttta ttttttcttt ttctgatact ctaaccgaat ctcgtaaatg tagtactcca
    20221 ctataaaggc gtatatatgc aagtaagtag ttcagttttg gagtacaaaa tgacaaaatg
    20281 tacttcaaaa tggactactt ttttcatttt aacttacata ccaaattaca tacaaaatga
    20341 tttataaaat gcaattgaaa atgataattg aatgaaaatt tgtgattttg tggtattatg
    20401 ataatataac ttatagaaaa aattgcttat tcaactaacg ggcaggataa tgagaaaagg
    20461 ttttttgata gaaataagaa attagatggt aaattagtta aaaaggctgt gttataaatg
    20521 aaagtttctt tgattgctgc gatggataag aatagagtga ttggcaaaga gaatgacatt
    20581 ccttggagga ttcccaagga ctgggaatat gttaaaaata ctacaaaggg acatccgata
    20641 atattaggta ggaagaacct tgaatcaatc ggaagagcct tacctgacag aagaaatatt
    20701 attctgacga gagataaggg gtttaccttt aatggttgtg aaattgttca ttcaatagaa
    20761 gatgtttttg agttatgtaa aaacgaagaa gaaattttta ttttcggagg agaacagatt
    20821 tataatttgt ttttccctta tgttgagaaa atgtacatca caaaaataca tcatgaattc
    20881 gaaggagata ctttttttcc agaagtgaat tatgaggaat ggaatgaggt atttgcccaa
    20941 aaagggataa agaatgataa aaatccgtat aactactatt ttcatgtata tgaaagaaaa
    21001 aacttattga gttaacgggt gcgttaattg aagaagaaca gtaaagataa agcacaagag
    21061 cttgggatta ctccaagctc tttttgtcat ttgaaataaa ttatatcttt ccaatcaaat
    21121 tgtttatctg taatccctaa tctttgagca ggtgtcaaaa cctttttatc cgctgattta
    21181 aaagggcgac aaaagttgta gtaagttctt agaattgtaa tcgcaaattg agcatacttc
    21241 ggattgaagt tagcgtaaat atagctcttc ttatcaccac gagctgttgt taatggtctt
    21301 tcaagagatg atatataacg tcttatctgt tgaataaagc tattggtcga atggtcatta
    21361 acatttaata gcatctttgc gatttcgttt ggttccagag cagataaatc cgttctacaa
    21421 tcaactgaat aaaatccttt atccttagtg gctaatggat gtttgatagg gttatcggca
    21481 tatttgcgat atctttctcc atctttacta atagcctctt cgtggaattg atgacttgtt
    21541 agtaattcag ttagataatg agatgccaaa gtccgtagaa actttgtttt gaatcctctg
    21601 atatctcccc aatcgagcag ttctgctttt gcttgtccga attcttttaa gcattgtttt
    21661 ctgcttttcg tcttgttaac ttggcaaata aaatgatgtg catcgctcaa ccttaattct
    21721 ttcgtgaata ctctataaaa ggcgtttcta atagagaagt catcatcact aatcattcgc
    21781 cattcgtcgg cattaaccat ttgtttaatt aaccaatagt gagcagttgt agtataagga
    21841 gcatcaacat ttaaaccatc gacaaatcta ctcctattag taattttccc taattcgtga
    21901 agataagcat tcctattttc gctatcgtta gcggatggtt cttgtggata ataagaccaa
    21961 tctaacctat catttttacg actaaaagta ttgagatggt cttctttgaa ttttcttgta
    22021 tcttcattta attcatccat tgaaatattc caatcatagg caacatcaga acggaacaca
    22081 tatcttgata agacctcagc agatacaact atatacgttt gcatattaag gtcttcaaac
    22141 cccgtatatt tctttgaacc ttgtcctttc ttacggacgt tatttaggta gtagtgcatc
    22201 ttgtccgtat taagccacat ctcgttaaat ttctttgttt gaaggggttg tgtctcatat
    22261 cgttctaaaa actccaaaca acgcctatac agccactcca gcttatgata gtaggttcca
    22321 actccaattc ctaaaatatc acaagtacga ctgactgaaa cccttccaac aaccatctta
    22381 gcgaacattg gaagaatggt gttcttttgt tggtgataag tagtggtttc ttctcttttt
    22441 ggtagaacat tagtgaactt cttacacgcc ttacattgat agcgttgaga tttgccccta
    22501 cttttccccc ttttgtaaaa ttgcttcggc tcattgaatg gggttgattc ttcttccgag
    22561 cagccttctt tatgaaattg ataatctggc tcaacatctt ggatgctatt gatttctata
    22621 aggcgtttaa tttcttcaac aacagaccaa ttagataaag gggttactgt acagttttgc
    22681 gatacacctc tgccaatagg attgtcattg cagtacatac ctttatgacc tttatctttg
    22741 gaactaccag tcattttata tcgacttggt ttccctttta catctttgaa tttgtgctgt
    22801 tctttcccaa aattacaaca aaacggattg atgcagtaat tatattgaat gggatattca
    22861 gaaccgttcc atttaaaaga cgttggcaag aacaacattt tgttatacct tgttgcaaat
    22921 cgttttggag ttaataaatc atattgttta gccctatctt ttaattcttc gtttgaaatc
    22981 ggaattttaa catctacaat ttcatcttcc ttcgtggcta atcttttaag cttggtcatt
    23041 gtctatcaac ccactctatc tttgataccc attttttcaa caatccgctt attacttcgc
    23101 ttgttggcaa tccattcaat aaacttctta tcgtctaaaa tgttcaataa gaattcatcc
    23161 actgcttcgc tttcagtacg ctctgcatat tcagcgtatt gcttaactat cacccttact
    23221 tgctctgata tttcccaatc cacagactta gcgttcttat tttttggctc aagaaattgc
    23281 ataggaaaat gcaccccttt ttgcttagta aattgtaacc attttaacac accaatttgc
    23341 attcctacaa taattatttc acgttcgcat ttttatatac attttaactt acattttgtg
    23401 gtgtaaatta acaacaaacg ttgatttaac aataaaaaac gagtatcaaa ttcagttgca
    23461 atttgatact catttaggtt tacattttca ctttttgtac tccaaaactg aactacttaa
    23521 tatgcaagac gttgtctttt ttttgtatat tcaattttga aaacatttcc gcaaagttca
    23581 gacggacctc gatgtgttga tgaataaaat ttattagtaa taacttctat aaggttatca
    23641 atccctatat tactttttgc acttccatga taaagaggga acagagaaca attctgaaat
    23701 cttatgcttt cctcttgttc gagttccaat gcttctaatg atttaccgga catatatttc
    23761 tctaaaaggt catcgtttcc ctctattacc gtatcccatt gttcagattc ggtaaagttc
    23821 gtcacacaca tattaggata cagttctacc ttctgtttga ttacaatttc ggcagaaagt
    23881 ttctctttaa tatcctgata aaccgttgat aaatcaattc cattttggtc aatcttattg
    23941 ataaaaaaga ttgtgggaat ccccattttc ctaagtgcat gaaataatat acgagtttgt
    24001 gcttgtacgc catcttttgc agaaatcagt agaattgccc catctaaaac tgataatgaa
    24061 cgatatactt ctgctaagaa atccatatgt cctggcgtgt ctatgatgtt caccttcgta
    24121 ttttcccact gaaaagaggt tattcctgtc tgaattgtaa ttcctctctg acgttctaaa
    24181 agcgtattat ccgtcctcgt tgtacctttg tccacgcttc ctaattctgt aatcgctcca
    24241 ctgttatata ataagctttc tgttaaggta gtttttcctg catcaacatg agctaaaact
    24301 ccaatattaa taattttcat gtgattttcc tccattcaaa aacccaaaag ggcataaaaa
    24361 tcccagtgat aaatactttt atcactggga tttttatgca taaccatagg tatacaaagc
    24421 atacagatat tctctggata ctttagaatc acatgataaa ggtattctta aactgggtac
    24481 aaaaaactaa gccctcctaa aaaaggacat ctaattattt gttcccgcta tcaaattgac
    24541 agtttattta agaatacctt gccgcatatt tattaactcc ttttaaatag tcacttaaat
    24601 aatagcacgt aagagcatat ttgtaaagga atctccaatt ttttatcaaa aagagtacat
    24661 gattacaaag tatctgtaat catgtaccaa tatttgttat tttacaatct tccaattatc
    24721 ttgtttttct aatatcaact caaattgcga gatttgggtt gcctttgttt cttgatctaa
    24781 atatttcaca gcgacattca cgattacttg gttatctttt ctggtaaaga taggattcac
    24841 caattcctcg aaaacatatt ccttgttaat cataggtaac acatgattac ttacatagta
    24901 agtcaattct ttttctgtgg cggtaggata gagctggaag aacgtttcta aaaagctagt
    24961 gatttcgtcc atcgtttctg tatctactgt atggtcactt tctagctgtt tgggttggta
    25021 gtccgatttt tgaggtttct tactcattgt cggatttttg ataataacca tattatctga
    25081 ttcatctata tggacaacca catgaaagct agacgatata gtttccttgt ctttgtcttc
    25141 ggtaataact tgttcaacag aaaacagtac ctcaaaggtg ttctcattca cttgagaaac
    25201 ctgccacacc tgtatatcgt tcacactaga actagtagga atatccttac gaatcatttc
    25261 ttcatttaat acctgcaatt cttccgtcaa ataatgggtc aatttttcat ttcgtttatc
    25321 aatggcttct tgggattgtt gccacgaaaa gtagtctttc gcaaaggatt tcacaaaact
    25381 ttctacttgg ttggtatcga caatctgctg tttaatgatt tctgtttcac ggacagtatg
    25441 agtgtcaatg gcagtaaaat ttttatagat tccaaatccg acactaccaa tcaataaaac
    25501 ccataaaaca aaggttaatt tcttatgcgt gccaacttta atcatcggaa cttttctttg
    25561 cttgctcggt tttttagatt tctttggttt ctccttcttt tcttttcgct cgatttttat
    25621 aatcatttga ttcattcctt tcttagttat gaataacacg tctagcccca atcagatggt
    25681 cttgccaata acgactgctt aagtcgccat aaccgattgg gtcgcctgca tgataaaagc
    25741 gatttccctc taaatatatc gccacatgag tcacatatgt gcctgcatta taggttgaat
    25801 gaaagaagat aaggtcgcct gcttgggctt cttccataga gatttcttgc gtagcgtcat
    25861 actgatcttg ggcgacacga gggagagaaa tgccagcttt gtcatacacc cattgtatta
    25921 agccactaca atcaaaagag gtggtaggcg acgcccctcc aaagacatag ggaaaccctt
    25981 cgtatttcaa ggcttcgtcc ataatgactt gcacggtttc atcatcaaat tctgttgggg
    26041 aagtgtcggt taggtattgt gaaaccaact gaacataaaa ctgatttcca tagttatatc
    26101 gccaaccacc attcacaggt atggctatag gattgggata gtctgctttt tgaccacccg
    26161 acttctcttt agaaaactgt tcggctaatt cataggtata ttttttccca tgactcctca
    26221 cataatttaa aaagccaccg ccataattat aagattggat aacagaatct atatccactc
    26281 cttgttgttc tgcactggtt aataattcac tgaaatattt aaccccttgt ttgatggact
    26341 cttcggtact caaactgttg ggcggtaaac ccaaagattc ggaactttgc ataacatctt
    26401 ctgctgtacc gcccgattcc acttgcataa tggctaagat ataagaaaca tagtcttcaa
    26461 tcccatattc cttaccatat ttttcaatca acggacggtg tgccaacact tctggtgaaa
    26521 cggtcacacc gccttgtgga atagaagaat ccttgttgtt ttcgccactg tccgtatcat
    26581 ctgcaaagaa aatgactagg aataataaga gaaaaaagaa gagagagaag accacactac
    26641 aaatcactgt tatttttagc ttcatgtttg ttgtcctcgc ttatccgttc ttttatttag
    26701 cttcctctga ttgaattgct tcggtggtgt cttttgtttt tgctcttttt tcaatggtcg
    26761 ctgatgattt tcacgtttag tgttttgtga caaaacaggc gtgacggttt gctgacgctt
    26821 gcgaacatgc tgctggttat tctgaacagg agatgatggg ttttcttttc gtcttttttc
    26881 ttttgcttta tccagctcca aacgcttttg agcaatgttc tttcgttttt gctcctgctg
    26941 gttcatacgc tgttgtttgc gttgagcttt ggtatctgcc atagattgtt taaagtctgt
    27001 tacattatga gtgacattct cttttccttg atgaagggca tatttgacat gggttggtgt
    27061 cgcctgtatc tgttgttttg cctgctttct tttattgact agacgatttt tcgtgtccaa
    27121 tacatgggct gtcttttcac ctaatcgttg cccgataccg ccttgttgct ttttcacatt
    27181 ggaaagtgct gtttctttct ttggtgtagt ccgttcattt tgacgagtat ggttagcttt
    27241 attggtttga ttctgctttg ttgtcattgt cttttgcttc gttgaccctg cttttccacg
    27301 aaaaatctta ctcatactgc gttgaaatct tctggtttgt cgattcatca tttgacgagg
    27361 tctgcgaaac acttgtctgc ccatgttttg agagtcgttg ctttgtaaag tgaataggct
    27421 cattaaatcg cccagtttaa aataaatccc tgcaaaggtc acaatttgta aaaacataat
    27481 catgaaaaag ggagaacttg ctgacagcga ataaagcata gtggaaatac taaaggcaac
    27541 ggtaataatc aaggtaatac cagcccttgt cataatcaca ttaaataact tggtaatggc
    27601 atgcctgccc atgccatcaa aactcggcaa catacttaat aggaaactga ttggcaaaaa
    27661 catagcgaaa ataataaata gcacttgcga aaaaatcatg attcctgtta gaaggaaaac
    27721 aaaaatcgag ataccaatat tgaatacaaa caaaaagaag actgttccta atctggaaat
    27781 cgtcttcggt agggttaaat aggtattgtc atgatcttca atttcttcaa tcactacatt
    27841 ttctctatct tcaccattgt ttgtatctgg actggtagac aataaacttt ccacacgttc
    27901 ttcccctaac gcttctacat cagtggagcc atattgaagc aatagccatg gttgctttac
    27961 ttgaacagag aataagctgt cacgtatcat gtccacactg tctttccctt gactatccgt
    28021 gttaggaaga acaatttttg tcccaatatt cagactggcg gtactgacat ctttggaaaa
    28081 atcattgatt ttcgcaatat aatcgggtgc atacgcaata aaggaagccg atagaataaa
    28141 caccacgaca aagtttaaaa cagctcgtat cactttcgtc gtttctcgct taaccaatcc
    28201 tgtataggca acatagattc caacaatgag aataaaaaga aggaggaaac cgacataaaa
    28261 ccctgaactg gaaaagccat tcggtgtaat gcctgcaatc gtctgcatgt tttcgccaat
    28321 tgcttgggat gtttgactaa tgaaatctaa ggcataggct tctttcacca aataacctgt
    28381 ggcgttcgat agataaagag aaattgtcca aataaaatta gtaatggcat acaatccata
    28441 catcaccgat tgtcctatgc catcactcca attccatgga agccagcccc agctattgtc
    28501 cacataataa tcaagctgat attgattcaa accatacttg gagtattcat tgcttgtatc
    28561 aatggtttca tcaactaagc ctgtcgcttg tactgcattg cccagcgtgc ctaataaaag
    28621 taaaataacg cccacacaaa ggagcgttat ccaactatag tgaagaaatt tcttttgttt
    28681 ttgtgtcatt cttccacctc atttcgcaca ggcggtctgg tatcaaaggc atgaagcaac
    28741 tcttcaaaaa tcgggtgaaa ctgcataacc cctacacgac catagatgtc tgaaactaaa
    28801 cattgtccat tttctaattc acgtaagcgt ttctgattgt tttcatcttc cttgtccaca
    28861 ccaaaaaatt ctaaggtctt tttgatttcc actaggtcag tggaacgaaa ggcaaatttc
    28921 aagccaatat tattcttcat cttttcatct agtaaatcgt ccgcgttttg agtaacgaaa
    28981 tagacccctg cattcatggc acgtcctgct cgaaccaatc gcatagatag agcttttcct
    29041 tgtgccactt gtaaaaaact ccaagcctca tcaaggtcaa caattttaaa aatggaacgg
    29101 tcagagtgga taaagtctaa agcaaaggta ctaatgacaa tcagcattgc cacacttaat
    29161 aattccatgg tagtgtattc ttcaaaagag ctattggaat cgggtaagac caaatctgcc
    29221 acttgaataa tattgagttg tttttccaag ctaatggatt gggtgacact gccatcactg
    29281 aataacagat gggcaaaatc ataatcgaca aagctttcga tatggtcagc aatactattg
    29341 ctgatgggcg tatcttcttt tcgcaattca tcaatcacca ataacaaccc tcgttgtttg
    29401 ctttgagtta cactccgaat agctttacgc aggacaggaa acttctcgct atctcggctg
    29461 gaaatacctg ttaagaacgt gagaatatca atggcaaggc tttctgaatc tttcgggagt
    29521 tttaaaatca cataagggtc aagtaaccct ttattgtcgt tctcactcgt gagattaaca
    29581 atattgattt catgggcaat atcaggtaag gtttctttcc attcaccacg ttcagctttt
    29641 gggtcaacaa tggttgcctg tccgccaaag agtactgaat aatagaccag catattgtta
    29701 gagaatgatt ttccgccacc taatgaacca ataaaagcgg acgctaatgc atttgttaca
    29761 gagcctttaa ttccttgact tgcaaggcta ggttggagat aaatatttct gcctgtatcg
    29821 agattgtaac cgacgtaaat accagaattt tcaccaatca tttgtgtagc accgaaacca
    29881 agaccagcca aaaaatccga cgtgacatat tgaatatagt cattgatata tcgcttgctt
    29941 gagggaataa attcattatg caagcctagc atatcgccaa aaggtcgtac taatttgata
    30001 gacagatcat cataaaaatc ttttacttca tcacaacgtt gtttcagctc gtccaaattc
    30061 tttgctgata cccgtaccac atagcttagt ttgtacatag attctttggt ttggtctaaa
    30121 ttactttcta attcatcgac agattctaaa gcgtccacca cattagtact ggtttcgttg
    30181 tcgctttccc atgcgtggct atctaaatct ttcagttctt tctttttatt gcgaacggta
    30241 gataaggctt ttttattggc gacaatttca acattcatac tggtatcaac ggggaaagta
    30301 aactgctgtt gctggtaata aaaaatttca ctattaggga aatccaattc accaacaata
    30361 ctattaattg taaaatacgc cacataagta gtggtatctt cctgttcaat ccgtaaatag
    30421 cgttgatttt cttcgattaa acagcgagtg ggtttgagaa tatcgtattt ctttaccaac
    30481 acttttttat cttcgtatcg tttcggtaaa tggtagtcgt agtcatcaaa agggacacct
    30541 gtttgcccgt aaagatgttc aataatataa ccaaaatcat ctttctccaa tttacgaatc
    30601 tgaaagcgtc tggataattt actttctaac aactgttcca tcttagaaaa gcgttcgatt
    30661 tccgccatag gcatagaaac aaaatcgccc attaatttgt gattcacacc atgaaagaat
    30721 tccgcaaatg ctttttgaat ttctttccca acatttttta cggatacttc ctgttcattg
    30781 aggtagagtt taaaaccaat aaaaaagcga tagtctactt gattgtcacc aatcatagaa
    30841 attaacgctt cggtctgttc atctattttt tcgtaagcta tctcttttaa tcgtccagtg
    30901 atttcttttt tcgattgttc ctgtgtggct cgaatacttg attcggttgc tagttgcaga
    30961 gcatgaatct tgccatcacg attttgggca atcaattgac agaaattatc atgaacttga
    31021 attttttcat cggaacttaa aaaactgtaa ttatagggaa ttaattcata ataagcgaat
    31081 acttcgccat catgattaaa tacgagatta tcttccatat atttaatcgg atatgccatc
    31141 tagttgttca ctccttacaa tcgtaatcga ttcattcatt gtttcttttt ttaatcgtat
    31201 ttgtttgcca gcgtaggtta ctttagggcg tagagcatag gtaatgatag actttagaaa
    31261 tccatagggt ttctttccgt caaaggtctt ttggcacatg aacccagtga gaacaatggg
    31321 aatccctaag tatttgagta gtgctccgtc aatcagagaa agtggtggta aactgccaaa
    31381 gagtatgacg acaaacaaag aaacgataaa ccatgtcatt tgcgtaaagg taattgggaa
    31441 agggagttgt aaatcattaa tcgcataaat cactttttct actgaccaaa tactggtgta
    31501 acttcttatt tttttcatgt cttcacgtcc tttccgtaaa aatagaaaaa gaacaatcaa
    31561 aataggtgat tgctctttca atgctttaac tattcaattt tattttttgt ccattttgca
    31621 ataatacctt catcagtttc ttcatcatct ggattcattg aatatctgaa acttaaattg
    31681 gtatctgacc ctaatcgttc atataagctg ggtctataca atctcgtttt attgtcatta
    31741 aaaggacttc gttttcccag ctcaccattt ccctttaaag gatttcttct aactgaaaaa
    31801 caatattttt ttctgggatc ttctgaatca ctttgactta atgagaaaca catggcttct
    31861 ttttggtctt catttttctt ctcactaact tctgtcggaa taaatcgagc aagagtttgg
    31921 ctaaattgaa ataaaacatc tcccaagtta ctactgtatt cgataccaaa atattctctt
    31981 attgttttcg tgtctgtata taattttacc gaattagcat agacagctag catatctgaa
    32041 aaatcgtttt cctttaaaaa ttctagtttt aacagagcat attctggaac tttttcctct
    32101 ttttcaaata gcttaacaag tacaatataa ttttgctgtt tgtaacgaaa gtaaaaggaa
    32161 tctatcaccc agcctttatc tttcatgtct agttttaata gtttaagatt ttcaaataca
    32221 tttaccataa tttatacctc tccgtgctat ttattaattt aattataaca cggattgtta
    32281 aacttactga caatattcaa ataccccatg agaggtaact aggtaattac cctctatttc
    32341 catatcacgt cccaaagatt ggtagtcaat ataattttgt aaattggaag ggacttcacc
    32401 aagctgtcct gtttcttcca cgtaataacg ggcgacatct tccatcgaat cacaatcact
    32461 ataacaatga atatcctctt tgttttccag taattcttcc aagctactaa accacatgcc
    32521 ttgaatttct ttgagttcat tataaatagg tgtaccttcg atttcttgaa tggcttcaca
    32581 tagacggttg atttcactaa tcggcgtgta ttcgtccaca tcaaaaggaa gctcaaaatc
    32641 atgtattgca tattcttcgt agtcctcatt taaaccgatt ctttctgcca tttcttcctc
    32701 atcaattggt ggagtaaacc atgctccgac taattcacct tcattatacc gtcctaaatt
    32761 tgccacatag actttcattt ccaattctta tcacatcctt tttataaaaa tagtcataaa
    32821 aaaagtagca atcagttaat gacgctactt atcaaggtat ccgctaatat aatctgtaac
    32881 tgtacttgaa atacctatta ttagttttgc ttctctcttt ccaataatat ttgtattagg
    32941 gtgtgcaagt gaatgcctat ttcttgcttc attaagagag ctaatcattc catttgatga
    33001 ccttatcgtt gtcttcacta tatcagcaag ttcagtaggt tgaatttctt tttcaattaa
    33061 ttgttgtatc ctactatata atttagatag attttcgtct ctaggaactg taatttcata
    33121 tttttttagt atttcaatta aatatccatg aaaagcagtg tgaactctgt caaaggcacg
    33181 ttgatattca ccattactca taaaagactc agcgtcttca atagcttgtg aaaccacatc
    33241 actaattttc aactgactaa agttaattac ttcgtaattc tctggaagtg gtactaaaat
    33301 agtatccact aatacattat caccttgttt atcatatatg gagcttgctg tctgggtaat
    33361 caaatcttta tgactttcaa aaaagtctaa atcatcaaaa ggaactctta ttttaagata
    33421 tgtgcaaagc gtattccagc cctttttata tcctttataa taagtaaaat cgtcagtctc
    33481 atctaaagtt acctctgata ttttcatttt agaaacaatc tcgttacctc cttcataata
    33541 atttgaagag ctctttcttt ctaatgtttt aattaaaaga gatttaaatt gatcatttgt
    33601 aaacatattc ataaatcctt tcataacaaa atatatactt cctattttat catgaaaaat
    33661 atctgttaag caccaatgat tcggttaaat aagtttaata acacatcttt cacgccagct
    33721 gcattgaaca caagtccgac ggcgattaga gcaataatca agaagccaat gagttttgaa
    33781 aattctcgtt taaaacctaa aaacagtcca atgacaacaa tcgctaacaa taccaatgac
    33841 tgtgcattat ccaagaacca gttgtacaaa ttctgcccaa aattcatgtg attgtcctcc
    33901 tttccttaag atatttttct cgataataga tatagctttt tacacgcctt aataattgaa
    33961 aaggggttaa atgctctcca tagacggtta ctcgattggt ataattatta taaaatctca
    34021 ccttgtaaaa atcataggta tagatggtat caccagcttg ccgttttccc tcaataatga
    34081 gagacgtata tttccctgta ttttgtatat acgcttgaaa ctcaatgcca cactgaaacc
    34141 ctaacttctt taattctttt gttaaaaggc tcatgtaatc aaatcctcca tggctagatg
    34201 ttgttgttca attaatttct catgtttttc tgtcagtttg gcttcctgaa tcatggtgga
    34261 gataatggtt gtaccgttga gaacatctaa aatgcttgcg atttttaatg taggggcgac
    34321 ttgatgatgt aaccagttca atgttcgttc aaaagaataa ggctctggct gtgtggttag
    34381 gcgtaatgct ccacgatttt taccgataaa ccattcccaa cgttcattag ttttccaatc
    34441 actccgacgt ttagtgtcgt ctttatctgc aaagcggata tagcgattga taatttcaaa
    34501 ggctgttttt tccgcctgtt gatgtgccaa taaatctttc atggcttggg tggctcggtc
    34561 attttttaac cgaatttcaa accgattctt tacttctgcg tcttctaagg gaacatcatt
    34621 tttcacgtat tgctcataat ctttctcata tatacagaaa tagacttcac tttttaatga
    34681 gccaatataa agtgtgtttc ccatgtatgg tttttcatca cggtgaacca attcaccact
    34741 gcgataattt ttaaagcttc gaaagacgga aatgcattct tcctgtttac atttctttgc
    34801 caaatctgga atattcaaaa tccctgtttt atcgttgata gctaaatcca agcgtttgaa
    34861 aataccttta tgttccatgc aatcttgaaa gaaatcatac cagctccgat gttgagccaa
    34921 tagaaagttt tcaaattgcc gacagccttt tcctttcagt tctaataaaa cacctttact
    34981 tacatcatga gagactagga caacaatatc accgaaacga tagtgttcgg gataagaata
    35041 atagccaaag tcttcgtgta gcatataatc catattcaaa cgtaaaacct caccgataat
    35101 ttgagccaca tcattggtag ggaatcgaat ccgtacatag tcgaataaca tctccaatgg
    35161 gttatcggga ttgaagcgtt ccaaagcgtt cagtaaatct tctttcacct gttgagtagg
    35221 gactgccttg cctgtttcaa tatccgatag gtaaggtctg gtaatatgag aagcaaccgc
    35281 cagtttattt tgagaaacgc cataagccag tcgcttttct ttcaattcct taatccaaat
    35341 tttttcgttt tccattcgca aacctccaat ctaaaaaaga tgtaaactat gacccttgag
    35401 tttacacctt tcatattttc cgcaatcctt tgtaccatca acattggttg atgagaattg
    35461 cctgttttca gttgtatttg tgcccccctg ttagatagag ggggctaaag gattggcgtg
    35521 gctaacgcca caccaatcaa agcaagtctg aacctgtacc tttcacttcg tgcggtacag
    35581 gctcagattg ctttttttga atgacttttc ctatttctcc taagaaatcg tacctttttg
    35641 gtacaagggg tgtataaaac tcactaatca cactttttcc tgtatccacg taacctcttc
    35701 ccttgatggg ttttaagaaa aattgttttt gtgtatcgct tccgaacatc ataccaaagc
    35761 ccatttcaga catacgaccc aaagccacac ggaagttaaa gttgtcccga atcccatcac
    35821 taaaatattt tgcgtctgga cgttgacagg caagaattaa aaagaagcct gcttgtcgtc
    35881 ctaacattac aatttgtttg agtttatgaa taacggtggt actttcacga cctagcatat
    35941 ccatcattgc cacatattca tcaaaaataa gaaaattggc agacaaacca agataagcat
    36001 aattttctcc tgttttatag ttagggtgtt gtttcatggt ttcactgcga tcaatcatat
    36061 cttcgtaaaa ttgattgata catgccaaca tatcttcttt acggtaatag acattattca
    36121 tgaccgcccc taagtctgcc aaatcagcat ttttagggtc aagaatataa agctttgcat
    36181 ttgtatgcag gagagattca atcaatgtga gaataaaata ggattttcca ctaccagtgc
    36241 ccccagcaat taacatgtga gggagagaat cgaactccca atagaccgat tccatcaatt
    36301 tgacacgtcc atcttgagct accacgtctt caatgccaat tcgattgcca atagtatcat
    36361 aaagtaggac atactctaca taagaatctt tcaacgtttt ttccactaat tcacaataca
    36421 atcctgtttc tagtttgttt tctaggtgca aaagctgatc ttgatatttc cctaaggtga
    36481 tttctacatg aatatggatt agtccatctt tcattcgata atatatttta ggaaaatggc
    36541 ggattcgttg tttggttttc ttgctgggta aatctttaaa gaatccctct gattgagttt
    36601 cttcggcttc ataccaatga ttttctaaaa tcatacgagc taacttttga cggtgctcta
    36661 actgttttat agaatctata cgatagtgat aataaagcca gctgataagt ccgcatacaa
    36721 aaagagcagt gagaatactg ctccaaaagt atggcgtatg aagttgatag aaaaatgtat
    36781 ctaactgaac tgcttgccag ttcaccgatt gtaacgctct ccaatgaaaa gggagtagga
    36841 cgactagaaa aactaccagc aatgaaatta gtacaaaatg atagaccaag tgcttatcgt
    36901 tggctttgat tcgctctcct ctgtcatgaa gcaatggcat aaaaatcctc ctttccacct
    36961 atcaaaataa ttgtgtattg tagatacgag cctgtttatc aaaagttggt tgagaaatgg
    37021 cttcatattt ctgataagcg attcgctttt caaagcgtaa atttttttct ttcggataat
    37081 aatgacgtcg atagagagtg tgtcctaaca gttttttcgc ttcttttgtc aacggataga
    37141 gatagcgaaa catgcgtcca ttgattttct caatcccttt gtattcgcaa aatgcctggg
    37201 aaagccaatg ctttttctct acctgttcaa agcgagcatt ttcctctaat aaaaggcgag
    37261 cacttcgagg gtgtatcttt tcccatgact gcttatcacg atagacactt gtcttgaaat
    37321 acccgcagta aaagaagttg gacgcttgat agacatagcc acattttccc tcaattccat
    37381 ctgccagtgt atataaaaaa tgacagtccg tatgttcttt caaccactta attagagctg
    37441 aaagagcttg actgccaaaa taattggttt gattcatttc tggaagaaaa cacatctttc
    37501 caatttctaa ataatctgtg gtttgaaggg aactgtcggg aaagagtttg cgtatggttt
    37561 gtaggggttg cgtaccccag cctaattcca caacgcctaa taatttttct tctgaaaaaa
    37621 tccctagaaa gtatttacac aatctaggga gtatctttga ataatggtat tgccgaataa
    37681 aagaaatggc ttcatccttc ggaatttctt tcaaaatcaa tggtgtgttt gtcataagca
    37741 acacctattt ttcactttcg gacgcttctt ttttatattt ctgatgaggt tttgtctgtc
    37801 cttttaatac aagatcgtct gctttcacgt accaatcaac gtctgcccct cgaaaagtag
    37861 ccgtcgctac ggtatctgcc acaggattga ttaattccac ttctgcgttg tagtcaaatt
    37921 ctttcaaagg aacagaagcg gggatactga cttgtatcat gcgtccttgt ccttttgatt
    37981 ttaagtcata ggtacgttct ttaatttctt cggaaaccgt accatcttca ttctggatac
    38041 gaacctcacg acgcaaggcg gaaaatttca atgtgccaaa tgtctggtct ttttcaatca
    38101 caataccttc tgctaatctc atattttttc acctttcctt tcttatgctt tcaccatatc
    38161 gtctgcgtgc ataatgtaat tggtaaatcc tcgattgcca attttataac cttctgcggt
    38221 aatatgtggg ttgatgagtt tgactttttc gtcggattca aaatgtttct cgccagcttc
    38281 ggagggaaga atgacaacaa tatcatctgc tcgttgaatc gttgagtata aattgtaact
    38341 acgagagaga atggttggtc tgccatttat tcgacgttgt tcaatgttcc cttcaccagc
    38401 atattctaat tgtccaaatg ttttttccat attgggaatg acatatttaa gttccatatt
    38461 tttacctatc ctttctgttt ttatgtttga atgaatctta tttgtgtgat ggttttaaag
    38521 gggggagagc gaccttttga ttcttgattt tttattttca taagttcact tcctttcaaa
    38581 attgggtaaa aaaatagacg ctccgtactg agcgtccgat tcctactata tttaaaacaa
    38641 cgactaccta ggtattttaa atattataat caaattctat tttatctaat aattgagtgg
    38701 gataaagcaa gtctccttta ctcgtaaaat aacccacctc tgagaaaaga aataaattat
    38761 tttttgctct tgaacaaatt acgtatagta gttttctcgc tgaactaatt cctttatcag
    38821 aaacattaaa aatttcattc caatgaggaa ttttcccgtt tagaagatta atagcgataa
    38881 cagtatcata ctcatttcct ttaactttat gtgctgtagt aagtgtgata ccatctcgag
    38941 atttaaacat cttcttaaaa ctagatacac tactagataa tgaattattt tttatccgat
    39001 cttctacttc gttaataaaa aatagatagt gcttaaaaat agacgggtaa tttttctcgt
    39061 caatattaag attaacaaat atatgtttgg taacaaattt gtataaatca acaccgttgt
    39121 cgtctccact atacattctt ttcacactgt ttattagctg taataaatct aagtcaataa
    39181 aataatcttt aacttctatt ccaaattcat tttttaatat aacgccgatt gtaaaattaa
    39241 gctagacaac taaaaaagt
//
LOCUS       DAFCNJ010000235        15532 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.guided.059, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000235 DAFCNJ010000000
VERSION     DAFCNJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.guided.059"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..857
                     /locus_tag="LNE35_003063"
     CDS             <1..857
                     /locus_tag="LNE35_003063"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK6912121.1"
                     /translation="SAQALGLDLLDKPTDQTIRLASSYALGELIKDALLQEVEQIIVT
                     LGGSGCSDGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRRNVLVSKVKLIIAADVTN
                     PYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWEVDLDDIPGSGAAGGIG
                     GALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGKVPYGVAVLAK
                     KYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILGQTLS
                     RFFTILPK"
     gene            1115..2617
                     /gene="eat(A)"
                     /locus_tag="LNE35_003064"
     CDS             1115..2617
                     /gene="eat(A)"
                     /locus_tag="LNE35_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK6912122.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2645..3211
                     /locus_tag="LNE35_003065"
     CDS             2645..3211
                     /locus_tag="LNE35_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6912123.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            3422..4483
                     /locus_tag="LNE35_003066"
     CDS             3422..4483
                     /locus_tag="LNE35_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK6912124.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4486..5382
                     /gene="murQ"
                     /locus_tag="LNE35_003067"
     CDS             4486..5382
                     /gene="murQ"
                     /locus_tag="LNE35_003067"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK6912125.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5396..7378
                     /locus_tag="LNE35_003068"
     CDS             5396..7378
                     /locus_tag="LNE35_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6912126.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7443..7889)
                     /gene="nrdI"
                     /locus_tag="LNE35_003069"
     CDS             complement(7443..7889)
                     /gene="nrdI"
                     /locus_tag="LNE35_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK6912127.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            8100..9524
                     /locus_tag="LNE35_003070"
     CDS             8100..9524
                     /locus_tag="LNE35_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6912128.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(9705..10478)
                     /locus_tag="LNE35_003071"
     CDS             complement(9705..10478)
                     /locus_tag="LNE35_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6912129.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(10475..11380)
                     /locus_tag="LNE35_003072"
     CDS             complement(10475..11380)
                     /locus_tag="LNE35_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6912130.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(11534..11854)
                     /locus_tag="LNE35_003073"
     CDS             complement(11534..11854)
                     /locus_tag="LNE35_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBK6912131.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12328..13014
                     /locus_tag="LNE35_003074"
     CDS             12328..13014
                     /locus_tag="LNE35_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBK6912132.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13016..13960
                     /locus_tag="LNE35_003075"
     CDS             13016..13960
                     /locus_tag="LNE35_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBK6912133.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            14159..14803
                     /gene="pcp"
                     /locus_tag="LNE35_003076"
     CDS             14159..14803
                     /gene="pcp"
                     /locus_tag="LNE35_003076"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBK6912134.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            14882..>15532
                     /locus_tag="LNE35_003077"
     CDS             14882..>15532
                     /locus_tag="LNE35_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6912135.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKIS"
BASE COUNT         4955 a         2744 c         3361 g         4472 t
ORIGIN      
        1 aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac
       61 ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac
      121 aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat
      181 ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac
      241 ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg
      301 ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag
      361 gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa
      421 aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg
      481 gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa
      541 tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga
      601 tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat
      661 atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg
      721 attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg
      781 aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca
      841 ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata
      901 ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag
      961 actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt
     1021 cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa
     1081 ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct
     1141 gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga
     1201 cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa
     1261 tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta
     1321 tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac
     1381 ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat
     1441 cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact
     1501 tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat
     1561 ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt
     1621 cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag
     1681 tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga
     1741 tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac
     1801 agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt
     1861 cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc
     1921 acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa
     1981 agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc
     2041 tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt
     2101 cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg
     2161 tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg
     2221 agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa
     2281 tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa
     2341 caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag
     2401 tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata
     2461 tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact
     2521 gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa
     2581 agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca
     2641 aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag
     2701 acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct
     2761 gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt
     2821 tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt
     2881 tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg
     2941 ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc
     3001 tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt
     3061 gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat
     3121 aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt
     3181 agataataca gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata
     3241 tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata
     3301 atataaactc aacctttaga aatgaaattt aattatttta ataaaagtat tgaaattttg
     3361 tttctttaga tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa
     3421 tatgtttggc ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat
     3481 ccgccaaatg gcatccaatg gctttgtcgg tattttcact tccatgcata taccagaaga
     3541 tgatgtttcg gcatatcaaa agcgattgat cgaactagga agcgaagcga aaaagaataa
     3601 actagaattg atggtcgata tttcaggaga ggcattggaa cgagcgggtt tttcattaga
     3661 agatctaacg cctttgaaag caattggtgt aacaggttta agaatggatt atcatatttc
     3721 caatcagcag attgccgact ggtcacatca attgaaaatc agtttgaatg ctagcacgat
     3781 cacgcctaaa gatatcgatg aattgaaaga agcagaagct gatttctcac agattgaagc
     3841 ttggcataat tattatccac gtcccgaaac agggctggac aaagaatggt atcagaaaaa
     3901 aaatcaatgg ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag gagatacaga
     3961 gttgagaggc ccactatatc aaggattgcc aactttggaa gaacatcgag ggatccatcc
     4021 tcttgcaggc gctctagatc tattagcaag taacacggat attgtttaca tcggagatgc
     4081 aggtctgtca gaaaacgttc aggagcaatt tgcttctttc caaaaagaac agacagtctt
     4141 gctccatacg gaacctgtag atgaagaatt ttacgaatat atattaggga agcatacgaa
     4201 tcggcaagac gatgcaagag atgtcatacg aagcgtagat gcacgttttc gagagattcc
     4261 gcctatccca gcaagaaata cagctacgcg tatgaaaggg tctattactt tagataacga
     4321 aaagtatttg cgctatatgg gtgagatcca attaaccaaa tatgatttgc cagcggatga
     4381 aaaagtgaat gtagtagcta aagttatcaa agaagagcta ccattgatca accaaatcaa
     4441 agctggtatg aactatcaat ttataagaaa agaaggaaga taaaaatgag caaaatcaat
     4501 ttagagaacc taacaacgga acgcagaaac caagaaactt ttggattaga tgagatgagt
     4561 atttctcaag ccgttcaact aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa
     4621 aaagaactga caagtatcga accagtgatt gctaacacga ttgagtcctt taaaaaaggt
     4681 ggacgtctga tttatatggg agccggaaca agtggaagac taggtgtgtt agatgctgct
     4741 gaatgcgtac cgacattcgg tgtagaaccg gaaatggtca taggtctaat tgctggtgga
     4801 gaagaagcaa tgactgtagc tgtcgaaggg gccgaagatt cttatgagct agggcaagaa
     4861 gatctaatca agctgaattt gacaaaaaat gatatagtta ttggtatcgc agctagcggt
     4921 cgtacaccat atgtgatcgg tggattagat tacgcaaaat cgatcggtgc tgtcacagga
     4981 acgatttctt gcaataaaca agccgaaatc agtaaacacg cggatttccc aatcgaagtc
     5041 gatgcaggtc cagaattttt gacaggctct actcgattga agtcaggaac agcacaaaaa
     5101 cttatcttga atatgatttc tactatcagt atgattggta taggtaaagt attcaataac
     5161 ttgatggtcg atgtcaaacc aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc
     5221 atgcaagcga cagaagctga ttacgaaaca gctgaaaagt actttgaaga agcagatcaa
     5281 aatgttaagc tggctatcgt catgattttg acaaatagtg ataaagagga agctcaagca
     5341 aaattagctg aagcaaacgg atttgtaaaa gggaccttat gagggggaaa agaccatggc
     5401 aaaaaacgaa ctgactttgg aagaactggc aaaacaaatt tataaagggg caggcggcat
     5461 gggcaatgta gaaagcattg tccactgcat gactcgtgtg cgtatgaaga tcatcaatga
     5521 tgatcttgta aatgtctctg aattaaaagc gattccagga gtgcttggtg tagtagaaga
     5581 tgaacagctt caagtcatca tcggacctgg gaaagtgaac aaagtagcac agcaaatggt
     5641 tgatttggca ggcgtgaaat taggagaaaa actttcttct gtaagtacag ctacagcaaa
     5701 ttcatcagca tctggaaaag accgtgtaaa cgaacgggca caagaaatga aagcggcaca
     5761 aaaatcgaaa caaaaaccat ctaaaatcaa atcggtatta aaagacattt ctaatatctt
     5821 tgtgccaatg attcctgcat ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt
     5881 agcaaactta gtcacagccg gaacacttga tgcagcaaca tggcaacaat atatcgatgt
     5941 gatgaacatc ttgaaaaacg ggatgtttag ctacctagtt atctatacag gtatcaatgc
     6001 tgcacaagtt tttggtgcaa caccaactct tggtggagtt atcggggccg ttgtgatgct
     6061 gacagggatg aatccggaag caccactgaa aaacttattt actggtgaag ctcttgcagc
     6121 tggacaaggt gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa
     6181 aaaactgcat aaagtagtac ctgatgcggt agatatcatc gttacaccaa ctctttcatt
     6241 gattgccatc ggtctgattg aaattttctt catcatgcct ttagcaggtt tcatttctga
     6301 tggcatggtc ggtgggatca actgggtact tggcgttggt ggagcatttg ccggcttcgt
     6361 tctaggcaca ttattcttgc cgatggttat gttcggactg catcaaattt tgactccaat
     6421 ccatgtgcaa atgattgatg aaactggtcg tacgctattg cttccaattc ttgcgatggc
     6481 tggtgcagga caagtgggtg cagcattggc attatggatt cgctgcaaaa aagataaaga
     6541 attaacagaa atgatcaaag gagctttacc agtcgggatc ctaggaatcg gcgaaccatt
     6601 gatttacgga gtaacattac ctctaggacg cccatttatt actgcttgta tcggtggagg
     6661 tatcggcggg gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc
     6721 aggagccgct ttgatcccat tgatctctga cggtaaatgg ttaggctaca tccttggatt
     6781 attggcagct tatgctggtg gcttcgttgc tacattcttc ttcggtattc ctaaagaccg
     6841 tttagaaaaa gaagaattag cgactgaaac agccacagta aatgaagtgc ctgaaaaagt
     6901 agtacctcaa acttcagctg aaagtgtgac tatcctctca tcagttgctg acggaacagt
     6961 tgtttcttta gataaagcaa gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta
     7021 ctttgtagaa ccagaaaacg gacatatcta ttctccagtt actggggtag tcagctctgt
     7081 cttcccaacg aaacacgcta ttgggatcac aacgccaaat ggtttagaaa tcttgcttca
     7141 tatgggaatc aacacagttg acttaggcgg tacgccattt gatgtgaaag taacagaagg
     7201 gcagcaagtg acacctgata cgcttgtagc agaagctgac ttagaagcga tcaaagcagc
     7261 aggcaaagaa acatccatga tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt
     7321 cttggaaaaa acaggtaaag caaaagcaaa agcacctgtg atggatgtca aagcttaata
     7381 tgatagaaac aatagaaacg aaagaagaaa ctgaaatcta tttttcagct tcttcttttt
     7441 tattatttat tgttttctgc taaaatctga taaattcgtg tcacatcaga tggtgttccc
     7501 cgtaattcat aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt
     7561 agacaatact gattattgaa gtttttattt ccgctaccga ctacaccaag acaaagttta
     7621 tgattgtctt catgttccag atattcacgc atcgtttcgg tcaagatttc tgtatcaccg
     7681 ttatctaaac catttccgcc gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt
     7741 tctttcgtaa aatcactatt ttcatggatt tcttttaaag taacttcagg attgacagga
     7801 tcttctgcat gcattttttc agcatattct gctagatgtt tggcaaaggc acgcgtattt
     7861 cctgaaatcg atatatataa aatgttcatg gatcatcctc caattctttc cttttagtat
     7921 agcatctttt ttaagttttt tggtggtgta atttggaaag ggactaggcg ctttgcttgt
     7981 ataatgagaa aaaagccggt agaattatat ttctattaaa gtttttatta attaagtaaa
     8041 aataaaaaag gtttgttata ctttttgata aaagcggttt tattttggag gatgcttaca
     8101 tggatacaga gcaacagaaa aaaggaattg gtttaattcc acttgctgcc ttagtaattg
     8161 gttcagcgat tggaggtggc gtatttggta ttatgacgga catctccgga tcggctggcg
     8221 gcccagctat tttcagttgg gtactcgtag gaacagcaat gttgatgctc tccttgtcga
     8281 tcaataatat caataaaaaa cgtccagatt taaaaggcgg gatctttagt tacgcagaag
     8341 aaggttttgg ccgtttcgct ggttttatca gcggttgggg ttactggctc acatgctggt
     8401 taggcaacgt agcttttgca acattgttga tgagcgcatt aggatatttc ttcccagtat
     8461 ttgaaggtgg gcaaaatctg gcatcgatcg tgatgagtac agtcttttta tggggatatg
     8521 catggctagt gaatcgggga gtcgaaaatg ctagtttcgt caatgctatc gtcacattct
     8581 gtaaattagt gccattattt ttatttattg tagtagctat cagcacgttt tcgatccaac
     8641 aattcattga taacttttct agcgacatta tattgaatgc gaatggacag ccgatcccgt
     8701 tttccagtca agtattgagt tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag
     8761 gagcctctgt cgtaggttca cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc
     8821 tagggatatt cggcttgatt attatttacg tcttggtttc catgcttcca tatgggacaa
     8881 tgacgatgaa tcagctgcaa acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa
     8941 tgatggttgg taaatgggga gcagtcgtga tcaacggcgg tttgatcatt tctttagttg
     9001 gtgtttggct ctcatggacg atcttaccga ttgaaacaat gggaaacatg gctgaagacg
     9061 gacttttgcc taaagcatgg ggaaaactca acaaaaatgg ggcaccaact tatgctatcg
     9121 tattgacgac gttatgtacg aatgtctttt tactttcatt attattcact gacagtgctt
     9181 ataactttgc ttacacactt ggtacagcag ctattttctt cacgtggctt ttcttaggat
     9241 tgtaccaaat gaaactttct tatcagcgga aagaatgggt ccaattttta gtagggcttt
     9301 tagcaagtgc ctttcaagta tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg
     9361 ttcttgttct tttcttgcca ggcatttatt tttacctcaa agcacgtaag gagcaaaacg
     9421 agcctgtaca acatatccac ttagataaga ttttacttat aggtgtgacg atggtcggag
     9481 tgattgcaat ggttttattt gccactggga tgcttcacgt atagaagtat aaaagaagta
     9541 taaagagagg ttgtgagcct gtcgtttatt caaaaacata ctaggaaagg agctgtgaca
     9601 caaatgttgt cacagctcct ttttcagaat aaacggtgtt taagagacag ccaatcggca
     9661 ataaggctga acgtctcttt cacagcctct tttttgatga gtcatcatac attttttcgc
     9721 ttaaatacga gatatcctaa tgctaagaac aaaacgatgt aagctaaatt accgaatagc
     9781 atttgattag tagaaagatg agtcaattgc ttcatgatct cttcgtttcc aatttggttt
     9841 tgcagattca acatactgat cggattccat ttgatccact cccatttttg gatgagtgca
     9901 ataaggatac cagatatgat tgacgaagca aaataaaaga cgattcctgc tgagatggat
     9961 gcgccggaac tattgataaa gcaagcaatc atcaacacta aacttaaaat cagccataaa
    10021 ccgatataac tacccaaagt ataagtgaac aagctttgga tcagtgtttg accagtagaa
    10081 accttctccg tgaaggaaat cgaagggaaa agaatcagtt tcatcaacac agtgacaata
    10141 atagctaaaa ggtataaata aacagaataa ataactaatg tgatccattt gcttactaat
    10201 atttctcctc gagaatactt tcgataaagg agatttttca atgtaccata ttgtgcttcc
    10261 atcgaaatga tcgtgcttgc agctgcaatc atgataaaga caatccaaga tgtggcacta
    10321 aaaagctgag cagtcatttc aactggatca atgatcgtcg tgtatttttt ggctagaagt
    10381 gctgttccta tgagcaaaac aactaataga acagataaaa cagcagttga ctttttcttg
    10441 atcaatttaa ataattcttg ttttacaagc aatgtcattt ttcttcttcc ccctttactg
    10501 aatccagaat ttccaataga gaagattcca aatcattcgt cagatgctgg acgtctgtca
    10561 catggatatt ttgtgcagac aatagctgga tcacggttcc taataattct tcgttttctt
    10621 ttaaaagctt gattttatca ggatcttcca aagtatatcc agcttctgtc aagatcgttc
    10681 gagcagctag atcatttgtt gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt
    10741 cagccattgt acattgtttg atgatctttc cttgattgat caggatcagc tcatcgacca
    10801 ttttttcaag ctcgctgagt aagtgactgg agatcaagaa agaggtgccc tgagtagcca
    10861 agtcggtaat gatttttcgc acatctcttg ttgcttgtgg atccaaaccg ttcatcggct
    10921 catctaaaat aacgatagac ggatggttca gtaaggcaag agcaattccc aatttttgtt
    10981 tcattcccaa cgagtaggat ttcgcttttt tgtgaatgta cttttccatc ttgagctgat
    11041 tcacgatagt gtcgatagca gatacatcgt tggtttcaga gaataacttc agatgatcat
    11101 agcctgttaa gaatggatag atacccgggt attcgatcaa tgctcctact tgttccaacc
    11161 ctttatgact agagggagaa atctcatttt caccaataca gattgttcca tctgagtaat
    11221 ggatcagtcc taaaatagct ttcatgatcg ttgtttttcc agcgccatta ggacctacta
    11281 atccaacgat tttgccaggg gataagctaa aactgacatc gctgagggct tgtttgtgac
    11341 caaagttctt ggataattct tggaccgaaa gacttttcaa aaatattcac tccttttcta
    11401 tagatagtac aagcatacac ctgatgagtg gagaagtcgc taggtcattg gacttatttc
    11461 ttattttttc ataaaaaaat tgaatattaa ataaaaaggc ttattttttc tgatcagtac
    11521 ttattccaag tgtctagtgg ccgctagtaa tcttgatgcc aatgatactg atgagtagca
    11581 tccccacaaa gaaccaagtg gaaagattca gcttatcatg aaacaagacc acaccaatta
    11641 tgaccgaacc aactgcgcca atccctgtcc agataggata agagagactc atcggcaaac
    11701 ttttgattgc ctttgacaaa aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag
    11761 aataattgat tttagaaaaa ccatcactcc atttcatcat cgttgaccag aatacttcca
    11821 aaattcctgc tatgattaat tccatccatg ccatacttac attcctcctt aactataaaa
    11881 aaatacacaa aaagaggaaa atcatccctt caaagaccta atggaatgat catcctcttt
    11941 atcctacccg gtggcagatt gtatatcttt ttctgtagta tagcacacct atccagatat
    12001 cggcaatctc atacttttta gtaaagtgta ccaagcactg acggatatat cgttagcgga
    12061 aaagaagatg aatgctttgc aatttcatag aaagaagttg ttatttatta atcatttaaa
    12121 aaaaacagta actagacctt atttctatat attatcgggc aataaattaa aaaaagcttt
    12181 accttacaat aagtaagata atttttgtga taaaaaaata acgaaaaata tttaaaaata
    12241 gtgtctattt cgtgagaaaa caactatatt ttttatgtgt tttgctttat aattaaaaaa
    12301 atattgtcat tttatggagg aaaatatatg gaaaattatc ttagcctttt aggcgtcttg
    12361 attgttattc tcgggtttgc atttaaatta gactcgatat tgatcgtaat ggttgcttta
    12421 gttgttacag ctttgactgg cggtcttggc attcaaggaa tgttagaact tttaggtaca
    12481 agttttgtaa acaatcgcgg gatggcgatt tttatcatca ttatgttggc aacaggaacg
    12541 cttgaaagaa atggtctgaa agaatcagcg gcttccttga tcaagcgatt caagaaagta
    12601 tctgctggta tgattgtaga tatttatggg atctttcgaa tgatctttgc ggcttttaac
    12661 gtgagttttg gtggagttgc tggatttgtt cgtccaatca ttttaccgat ggcattgggg
    12721 accatcgaat caaaagggtt agaagtccat cctaagcatg aagaagaact aaaaggtatg
    12781 gcttcagcta tggaaaatgt ctgctggttc tttggccaag tactattcat cggaggagca
    12841 ggggcgttgc tagtccaatc aacactgaaa gatctaggat atgacgtaac attgggacag
    12901 cttgctttag tggaagttcc agtggctatc acggctttag ttgtggcaag tgtctacttc
    12961 tatcttaaag aaaagaaatt agcgaagaaa tattatccag gaaaggggca ataagatgga
    13021 cttttttaca aatcctgata ttttattagg cgataaatta ctggagatcc tttatatcgt
    13081 gatgggactc gtattgattt acactggagt aagaaattta acagataaaa ccaatcctca
    13141 tcgttatggg tcagcctttt tctggacggt cttaggggta gttattgcag gaggaagatg
    13201 gcttccggca atcgtgaacg gtggcttgat ctttgcgatg accataccag caattgctaa
    13261 gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga
    13321 caaactagga atgaaaattt ttattccagc tttgagtatc ggtgtctttg cgatcttgtt
    13381 tgctctattt accaatttag gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat
    13441 gctaatcttg atgttctttt ctcgagataa tcgaccaagt acatttttag atgatgcagc
    13501 agatatgctg ggcacagtag ggccgttaag catgttgcca atgcttttag cttctctagg
    13561 tgcggtattt acaagcgctg gagttgggac agtgatttcc aatgcagtag gtacgatcgt
    13621 tccagaagga aatgtgaaca tcggcatcat catttacgct cttggcatgg tgatatttac
    13681 ggtaatcatg gggaatgcat ttgcggccat caccgtgatg acggttggga tcggtgcacc
    13741 attcgtattt agtcatggag cgaatccagc gttaatagga atggttgcat tgacttgcgg
    13801 tttttgtggg accctgctga caccgatggc tgctaacttc aatatcgtgc ctgtagcgat
    13861 gctggagatg aaagacaaat acggagtgat caaaaaccag ttgtttattg cgttatttat
    13921 gctagttttc caaattgctt atatgattct atttaaataa tagaagagag gggttgtgac
    13981 agaataattg tcgcaacctc ttctaacatt gtagtggaac actatttttt tataagaata
    14041 tattaagttt gatctgttat gttatttaaa ctattgtttc ctttatttac gctgttcaag
    14101 gaaaatggta tgataaaaga taaccatatg ttaaatcaat cggacggagg aacaacagat
    14161 gaaagtacta gtaacaggtt ttgatccatt tggtggggac aaagtgaatc cagcctatga
    14221 agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa atcatcaaag tggaagtacc
    14281 gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc atccaacagc accaaccaga
    14341 tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc
    14401 aatcaatcta gcagaagcaa gaatccctga taatgaagga aatcagccat tcgatacggc
    14461 tttagaagaa aacggtccag ccgcttactt tacctcattg ccaatcaaag caatgacaaa
    14521 aaatgtacaa gatcatggtc taccagcgta catctcttat acagcaggaa catttgtctg
    14581 caatgatatc atgtatcgtt tgcttcattt gatcgaaaca caattcccgg caattcgagg
    14641 tgggttcatc cacgtgccat tctcacccga tcaagtgatc gatcgtccag tgggtacgcc
    14701 ttctatgtct ttagaagaca ttgcttcttc tttgacttat gcagttgaag cagcagtaga
    14761 aaataaagaa gatatttccg gaaatgccgg aacaactcac taatttagca agaaacactg
    14821 gctggttgct aacgttatgt tagtagccag ctttttgtaa ttagaaagga aagaaacagc
    14881 cgtggaagaa tggcaatccg tctttgaaga atggtttcca aaagaaatca gcaaaagcta
    14941 tcccatcaaa atttctaaac aatacacaag cagtcaacgt tgggagatct atgcaaaatt
    15001 aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga tatctaatca gctctcgttt
    15061 tatggaagaa cattatttag ctgcaacaga ttgggtgttt tctgacttca agatcaatcc
    15121 gttttttcga acaaaacgaa gccagcaaaa attgtattgt gagtgtggta gagaattaaa
    15181 agtgcaatac atcgttaaat cgccaaaaac cggaaagatc ctaaaactag gcatcaacca
    15241 ctttgccgat cacctgcatg tctctccgac agtggcagct tcgatccatc agggaatgac
    15301 aaaagttgat ctggccttag acgaattatt atggcttaag cagaagaata tcgattttcc
    15361 tgaaggacta tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc
    15421 atatttaccg gatataaaat tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc
    15481 gatttacata gcggattatc aagcattgga aaatgaaatc aagaaaataa gt
//
LOCUS       DAFCNJ010000236        13213 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.guided.069, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000236 DAFCNJ010000000
VERSION     DAFCNJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.guided.069"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(204..2162)
                     /locus_tag="LNE35_003078"
     CDS             complement(204..2162)
                     /locus_tag="LNE35_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6912136.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="LNE35_003079"
     CDS             complement(2159..3031)
                     /locus_tag="LNE35_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6912137.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="LNE35_003080"
     CDS             complement(3373..4245)
                     /locus_tag="LNE35_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6912138.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="LNE35_003081"
     CDS             4373..4942
                     /locus_tag="LNE35_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBK6912139.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="LNE35_003082"
     CDS             5009..5746
                     /locus_tag="LNE35_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6912140.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="LNE35_003083"
     CDS             5977..7245
                     /locus_tag="LNE35_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK6912141.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="LNE35_003084"
     CDS             7732..8196
                     /locus_tag="LNE35_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6912142.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="LNE35_003085"
     CDS             8180..8809
                     /locus_tag="LNE35_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK6912143.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="LNE35_003086"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="LNE35_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK6912144.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="LNE35_003087"
     CDS             10688..11134
                     /locus_tag="LNE35_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK6912145.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="LNE35_003088"
     CDS             complement(11333..11848)
                     /locus_tag="LNE35_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6912146.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="LNE35_003089"
     CDS             11965..13167
                     /locus_tag="LNE35_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6912147.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAFCNJ010000237         9521 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.guided.081, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000237 DAFCNJ010000000
VERSION     DAFCNJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.guided.081"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            229..288
                     /locus_tag="LNE35_003090"
                     /pseudo
     CDS             229..288
                     /locus_tag="LNE35_003090"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
     gene            346..1077
                     /gene="erm(A)"
                     /locus_tag="LNE35_003091"
     CDS             346..1077
                     /gene="erm(A)"
                     /locus_tag="LNE35_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HBK6912148.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1203..1985)
                     /locus_tag="LNE35_003092"
     CDS             complement(1203..1985)
                     /locus_tag="LNE35_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HBK6912149.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2139..2516)
                     /locus_tag="LNE35_003093"
     CDS             complement(2139..2516)
                     /locus_tag="LNE35_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6912150.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            complement(2523..4415)
                     /locus_tag="LNE35_003094"
     CDS             complement(2523..4415)
                     /locus_tag="LNE35_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6912151.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            complement(4412..5497)
                     /locus_tag="LNE35_003095"
     CDS             complement(4412..5497)
                     /locus_tag="LNE35_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6912152.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            complement(5628..6179)
                     /gene="radC"
                     /locus_tag="LNE35_003096"
                     /pseudo
     CDS             complement(5628..6179)
                     /gene="radC"
                     /locus_tag="LNE35_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            complement(6234..6887)
                     /locus_tag="LNE35_003097"
     CDS             complement(6234..6887)
                     /locus_tag="LNE35_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6912153.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            complement(6877..8190)
                     /locus_tag="LNE35_003098"
     CDS             complement(6877..8190)
                     /locus_tag="LNE35_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBK6912154.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            complement(8500..>9521)
                     /locus_tag="LNE35_003099"
     CDS             complement(8500..>9521)
                     /locus_tag="LNE35_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HBK6912155.1"
                     /translation="GADALRWFLSNGSAPGQDVRFSYEKMDASWNFINKIWNASRFVI
                     MNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTELFDRFEFGEAGRQLYNFIWDD
                     FCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRLLHPIMPFVTEEIWEHIPHQG
                     NSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIRSEVNTPLSKPITLLIKTNDA
                     KIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAVLTGAEIFLPLAGLINIEEEI
                     KRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAERAKEKDYLEKQTAVKERIES
                     LRTIQ"
BASE COUNT         2874 a         1910 c         1425 g         3312 t
ORIGIN      
        1 gtaaagacag tggcttctct tatcaagttt caaaacatat tattttgaag aaaacgtcca
       61 tctgaagtgt caagtgcaaa attacatata aaggtttatt ctaaaatgaa aagatgatac
      121 aatcatattc agttacataa ggaggtttca attatgtgca ccagtatcgc agtagtagaa
      181 attactttat ctcattcata atgaaaaaaa tggaaaggag ataaaagtat gggtactttt
      241 tctatatttg ttattaataa agttcgttat caaccaaatc aaaattaatt ggttataatg
      301 aacgcttaat gtcagttcat tataaccagt aaggagaagg ttataatgaa ccagaaaaac
      361 cctaaagaca cgcaaaattt tattacttct aaaaagcatg taaaagaaat attgaatcac
      421 acgaatatca gtaaacaaga caacgtaata gaaatcggat caggaaaagg acattttacc
      481 aaagagctag tcaaaatgag tcgatcagtt actgctatag aaattgatgg aggcttatgt
      541 caagtgacta aagaagcggt aaacccctct gagaatataa aagtgattca aacggatatt
      601 ctaaaatttt ccttcccaaa acatataaac tataagatat atggtaatat tccttataac
      661 atcagtacgg atattgtcaa aagaattacc tttgaaagtc aggctaaata tagctatctt
      721 atcgttgaga agggatttgc gaaaagattg caaaatctgc aacgagcttt gggtttacta
      781 ttaatggtgg agatggatat aaaaatgctc aaaaaagtac caccactata ttttcatcct
      841 aagccaagtg tagactctgt attgattgtt cttgaacgac atcaaccatt gatttcaaag
      901 aaggactaca aaaagtatcg atcttttgtt tataagtggg taaaccgtga atatcgtgtt
      961 cttttcacta aaaaccaatt ccgacaggct ttgaagcatg caaatgtcac taatattaat
     1021 aaactatcga aggaacaatt tctttctatt ttcaatagtt acaaattgtt tcactaaatt
     1081 aaagtaataa agcgttctct aatttcacaa gaggacgctt tattcttccc aaaaattgtt
     1141 caatatttat caataaatca gtagttttaa aagtaagcac ctgttattgc aataaaatta
     1201 gcctaattga gagaagtttc tatagaattt ttcatatact taacgagtgc tttcaccttt
     1261 gaatatagtc cttcccactt atcatcacac tctccccgat agccttttct agctatatcc
     1321 agtaaagtta catgctcttt aggtaaaaga ggtatagccc attctgcagc gacatctttc
     1381 gaggtaattt caccagtagt cactgtttgc cacattcgag ctagggttaa aattacatta
     1441 cgctcatcac cttttatccc ctcaattagt tctggcaaag aatccttaat tgctcttcga
     1501 atatctgtca aaggtacgga gacaagtata cttgaagaat caggaccaaa tagagaaata
     1561 ctattctttc ttgcttgtgc taaaacaata gccaaatcag gatcatagct tggttcctga
     1621 atttgtccat tctcaaattc acccctgagc cactcaccgt atataaattc tctttttgga
     1681 ggatattgcc aagggacaac ttcactccta tttataaccg taacttcaag tggtctaaca
     1741 gaatccgtat ttccaatctt tcctgatata gtcattagtc tttctgttag tttttttcga
     1801 gttaattgag gtaaactatg attcacgacg actagaacat ctacatcgct gttaatgcgt
     1861 aaaccaccat ttactgctga accaaataga tatactccaa ctattgaact tccaaataaa
     1921 tcttttacga tttttaatgt ttgaatcgct tgatttggta tttttccgtt aatcaaattg
     1981 ctcatgattt cacctcgtaa ctgattatgt tcatataaag tttatattga tactcaattt
     2041 acttacccta gattggacat atacctaaat gactgttcaa taaagctgac cgttagcgtt
     2101 taagtacatc ctttcacaat ttgtctacag attaataatt attctttatt atacagatct
     2161 ccatataatt tttgaatttg gttctgtaat tttttatttt ctttttctaa ttccattact
     2221 cttcttttta aggttttaat aaggatttcc tccgaacgag aacttttctt gggttttgag
     2281 actacatttg ctgttatttg acgctcacga agggattcga ttctttgcct aatatcgtgt
     2341 tccttataaa gccatgattt agaaacatta gcttcctttg ctattgaatt aaaattaata
     2401 actttacctt caatcgaaaa tttagaaatc gctttgtcta ctttttccct tgtctttttt
     2461 gatttctgct tcgccaaacg tacaatttct gttgtatttc taacttgttt atccattgat
     2521 aattaccccg tcaaacttcc aatgatttgt tctaaacgct ctttaacacg gctattagtc
     2581 tctacttgtc tttgccattg tttatcctta gctatggcta ataactcttc tgtacgctct
     2641 aactgttctt cgtgctgtgg taagaattgc ttactggtac agaagtgagt gcaatctaag
     2701 catgcattcg catgtggaca accacctgct actactggca atctacaata accatttgga
     2761 agcacttgtg catttatatt tttcttgaac cattgaagct ctacatcatc gacttcatta
     2821 tcttcatcta gatcaagcac atctccatta ttggtaacca gtttttcctg aaatttagta
     2881 aattcatttt ttagagtttc atcaaagata tgagcgtatc tgcttgtcat ttctgggctt
     2941 tcatgcccca aaaatttctg cacaatatgc tggggcatcc cgttgttaat cattcttgtt
     3001 cctactgtat ggcgaaaggc atgggcatgg aatctataaa tctcacctga tttatccact
     3061 atattttgct cataagctaa tttatttaac tcacctctaa atgtttcttg ttttaatggc
     3121 gatccatctt ttcttggaaa gaggtattca ctatctggaa attcctctga aactttatct
     3181 tcccgaactt taataagtaa agctacctct ttagatattg gaactatatg ctcctttttc
     3241 attttccatt gataatactt taaaaagaaa tctccatctt tgtcctctaa tagacagcct
     3301 tttttcaagg tgcacaattc acttatcctc attccacatt cttgaacaat catagtcatc
     3361 gtagctatat attcgggtaa tttatcaaga tgactgttca attgctctag gacgaattca
     3421 tctataaagc gtggttttgc tcttggtatt ttcggatagt cctcagaata aattaatatt
     3481 ttggaaggaa catcatccca ttctagccta agaagggtac taaatagtcc ttccaatata
     3541 gagatcctcc cagttattgt actaggtttt attcccatca tgtttagttc acttaaatat
     3601 gcttcaattt ccactctcgt taattggtgt actctctgaa cttgtttaaa tttcatgtcc
     3661 agaaaattaa agaactcttt aagtctttgg gcaatatcac ttacatagga aaagctatcc
     3721 atgttcaatc tcaacttaca atatcttttt acaagttgtt taaaatatgt attccgaaac
     3781 cctttaaagt taattgtata ttcatattgt gttgggttaa ccttatcatc tggcaaaggt
     3841 aagttacgtc tatcccaaac gtctttatcc cactcctctc catcaaaata aaagttctca
     3901 taaaactcca taaattgttt tagattagta acatagtagg aattagcttt tacaggtgtt
     3961 ttttcttgat tagcagtaat cttataatta gtagtggtaa ttctaacacc ccgttttgtc
     4021 aaataagttc tatactccgt cattgctttt tcaataggaa cttcagtaat tgaagtaatg
     4081 ctaggatact ttaaatctaa gaaatctaac attttattaa ttactgttcc ttttctaatc
     4141 cagacagttt ttgcattcca tattccattg tttaaatggt aaaaataaaa atatttcaat
     4201 tctgttctta accacagatt tttaacacgt tcaaaacgaa cccaacgatt ccttaaagca
     4261 ggattcttac ttaattctat ggcagaagga tgtggacatt ttcttatatc ccaactatta
     4321 ttagcccaaa acccctgcat ttcttcattc attacagcta tttttttgct aatctcactc
     4381 tgactaataa ttttcctttt actagaagca ttcatttctt atgctccttt ctctcgaggt
     4441 atttattaaa ctcatttttc atatcctgat ctgaaagatg aacataggta tttaacgttg
     4501 tctgaacatg tgcgtgacct aatctctttt gaacgaacgc aacatcccat ccttccctaa
     4561 ttagctgcgt tgcgtgagtg tggcgaagca tatgtgatgt aaattctatt ccagtccttt
     4621 taactattct tctaactaga tcaagaacac tttggtactt tagtggtttc ccaaaatagc
     4681 cttcttttaa ggaaataaaa acataatcat gctccaattc ctcactatac tcatatatca
     4741 agtaatctgt ataaagtgac ataagttctt tactcacatg tattgttctt tccttcctta
     4801 atttaatata agcttcatta acattaacat ctctaggtgt taaatggatt tgattgtccc
     4861 aagtgacaat atcttcaagc ctaagcgata acacttcacc gattcttaaa ccaccctcat
     4921 acataagcat taaaattaat ttatctcttt tcgtatgaca agcatcaata atttgcttaa
     4981 cttcctctga tctcaatgtt cttatctgtt tctttttaac ccttaacttt aagacattct
     5041 tttggtatct acccttatta acatgatgta aaaatccttt gaaatttctt ccctttgctt
     5101 gtttaaatac atcaattgat ttaaattctc ctaatctact taaataatca agaaaactca
     5161 taactacatt taaaattgta ttcactgtcg tttcttctct tatggctttt tttgactgaa
     5221 gatcaattac atttgatgct gaaggatatc tcaaccaacc tacgaagtct gctaacaact
     5281 caaagttaat atcattaaga ataacacctc tctgttccat gaactcgtac agcaacttta
     5341 aatgatagca gtatgcttta atggtattag gagacttacc agtattatct aagtatttaa
     5401 taaatttcat tactggttct attagctggt attctttatc tagtaataaa tacaatggat
     5461 acggcttatt ctccacttct atcctttgaa ccttcacatg ttcccacctc tttaaatact
     5521 ttaactacta caaattataa accttgttaa tttttattta aagtaaaata tccctttaat
     5581 atctcctctt aaactacttt aactactatt atttattata ctatggttaa aacattacca
     5641 gaaggatgat tatgcgctaa aacgatcctt gctgcacaat aacgtacagc ataatggaaa
     5701 atttctctag gatgagcgac tgactgattc agcgaaccga tgaacactgt tttttttaag
     5761 atcacttgat tcttcgtgtc caaataaata caaaccaaat gttcttgttt gtgatctttc
     5821 atttccagaa tcaattgctg ggccaattcg tagctggtac gaaccggtgg agccgctctt
     5881 tcatgatccg tctgaattcg tcgacctaat tcgatcaagg ctttgatttc gattgctttc
     5941 acgcgtccta tccctcgaat ctcttctaac tcatgcaaag tagcctgtcg taaagaggcc
     6001 agtcctccaa acgtttttaa cagatttcca gcaatcgaca tcacactata agggtgttga
     6061 ccggtacgca gtaagattgc caaaagctcc tgatccgata aagcttccgc gccataaatt
     6121 tccatacgtt cccttggtaa agagctagta ggtacttctt tgatcaatcg ttttcccaaa
     6181 aaaacatctc ctttctatct tatctaaaat aagatacgaa aagagatgga tttttatttt
     6241 aacaatgcag ggatttcatg taaagaagaa acaaccccag ctgttttttt gatcaattct
     6301 tgggatggtg gaagcagatc tggtaccatg actaccggga tctcagccgc agaagctgct
     6361 aaaataccat tttgtgaatc ttctaaaatc aatgtttctt tcttcgctgt acctagcttc
     6421 tgattagcta acaagaaaat ttctgggtct ggttttgctc gttgaacatt ttctgcagaa
     6481 acaatcgtct caaatcgttc aaacagattg ttcttcttca ataaaagttc aatgactgct
     6541 cgttggttac tggaagcgac aactttggga atctgatgtt cttctaaaaa agagaggagt
     6601 tccaacacac cgggcttcat ttgtaccgct ccttgcgaaa aacgttcaag cgtttcctgg
     6661 tatgaatcat ctatgaatct ttgtacactt gttttgccaa attccgagaa aatcatatga
     6721 taattcgccc atacttcttc atctgaaaca cctaagaatt ttaaatacag gtctttatca
     6781 taaggaaaac ccatcttgtc tgctactact tgtgtggctt cgtaatacac taactctgtg
     6841 tcgaacagca aaccatccat gtcaaaaatc actgcattat acttcatgat ctcctcctaa
     6901 ttgtattaat aaatggcgaa cttctgaaat gaaataaagt gaaccggtca caactaatac
     6961 gtcttcattt gtcatctttt ccaatatatc tgctaatccg aattgccaca atgaaacgat
     7021 tgatactctc tcacttgcta aatctgatac ttcacttaat tcgatggctt ttggatgttc
     7081 aaaggtcgtc aaataaatat ggacatttgg tacttgctgc aatcgttcta gcatttctct
     7141 aacattttta gttcgtaagg cggaaaacaa aatgttgatt tgatatcctt tgaattcttt
     7201 tttcaaattc tcaacaagcc ggttcattgc atgcgtatta tgggcaccat ctaaaacaac
     7261 taatggctcg ttactcaaac gctccattct tcctggccat cgagatttct tcaatccctt
     7321 caaaacatct ttttctcgga aaggcagctt ttccaattga cagtaaacaa aatacaattc
     7381 gatggctact cctgcatttt cagcttgatg ttgccctaaa agcggagtgg ttaattgagg
     7441 gatctttcct atttcattaa aaaattcaaa tacttctccc cacatctcat caggatgcag
     7501 atagtttatt ttataatttt ccccgtatac ataaagcggt gcattttttg ctttagctgt
     7561 ttgttggatg acagtaaaag cttctggggc aatatttcct gttactactg gaacgccttg
     7621 tttgatgatt ccagcttttt gtgcagcgat ttcttcaatt gtatctccaa gtatttcggt
     7681 atgatcaagt ccaatcgttg ttattcccgt cagcattggt acagctacat tggtagaatc
     7741 caataagccg cctaagccaa cttcgataat cgctacgtcc actttcttca tcaaaaaata
     7801 atcaaatgcc attgctgtta atgtctcgaa ttcagtaata ccagctactg cttcaaattg
     7861 atcaagttgt ttgatcagtg gctgatattt ttttacaagc tggatcaaat cattgtctgg
     7921 aatgcttttg ccattgattg cgatccgttc attgaacgtt tcgatataag gtgaagtaaa
     7981 tgtacctact gtcagcccag cctcttctaa taggcttctc aaataagcta cagtagaacc
     8041 ttttccgttt gttccagcga tatggatcgt ttttactttt ctctctggat gccctaccac
     8101 ttctaataaa gcttcgactc ggtcaaggcc aggacgtgaa ccaaatggta atcgactatg
     8161 gatccattct attgcttctt caatcgtcat caacaattcc cccatttctc ttttattata
     8221 acaaatagaa cacataagaa aaataaaagt atgacaaaga gaaaagaaac agagagagga
     8281 acgattttat caactacttt attgactctt gatacatgat ccacctccct ccttctccta
     8341 cataataaga gtagactaaa aaagagatgc gacaaaattt atgtcacatc tccttatgaa
     8401 tcgaagctaa gagtgaggct caaactcttt cctgtaacga tgttcaatca gctagctttt
     8461 agtaatagct agctgatttc acactctctt tttctattct tattgaatag tacgcaaact
     8521 ttcgattcgt tctttgacag ccgtttgctt ctctaaataa tctttttctt ttgcacgctc
     8581 tgcctctact acttcttccg gcgcgttaga aacaaaacgt tcattagaaa gttttccttg
     8641 gacacgtttt acttcgcttg tccacttttc cagttctttt tctaatcgtt tgatttcttc
     8701 ttcaatattg atcaatcctg ctaacggcaa gaagatttct gcacctgtca gaacagctga
     8761 catcgctaga tctggcgcag tgatctcact tgatatcgtc aactcttcgg gattacagaa
     8821 gcgttcgata tagcttgtat tttcaactaa aaattgatcg attttagcat cgtttgtttt
     8881 aattagcaac gtaattggtt tagacaatgg tgtattcact tctgaacgaa tatttcgaac
     8941 ggctcgaatc aattctttca atacttccat ccctttagaa gctgtttcat catcaaactc
     9001 tggacgtaca acaggatatt ctgccactac tagagaattt ccctgatgag gaatatgttc
     9061 ccatatttct tctgtaacga atggcatgat tggatgcaat aaacgtagga tctgatcaag
     9121 tgtatggact aagatgctac gggtggtttg ttttgaaaca gcatcatctc cataaagtac
     9181 ttctttactc atttcgatgt accagtcaca aaaatcatcc cagataaagt tatacagttg
     9241 tcgacctgct tcaccaaatt caaaacgatc aaatagctca gttactcgtt cgatcgtttc
     9301 attcaaacga gtaaggatcc accggtcagc cactgatttt tctccagata aatcgatctc
     9361 ttctacggtc attccttcga tattcatgat aacgaaacgt gaagcattcc agattttgtt
     9421 gataaagttc caagaagcat ccattttttc atagctgaag cggacatctt gtcctggtgc
     9481 agaaccattc gataagaacc agcggagagc atctgctcca t
//
LOCUS       DAFCNJ010000238         8061 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.guided.089, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000238 DAFCNJ010000000
VERSION     DAFCNJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.guided.089"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            128..1564
                     /locus_tag="LNE35_003100"
     CDS             128..1564
                     /locus_tag="LNE35_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6912156.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="LNE35_003101"
     CDS             1753..4200
                     /locus_tag="LNE35_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK6912157.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="LNE35_003102"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="LNE35_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBK6912158.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="LNE35_003103"
     CDS             complement(5690..6793)
                     /locus_tag="LNE35_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6912159.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="LNE35_003104"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="LNE35_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK6912160.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2696 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DAFCNJ010000239         5613 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.guided.109, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000239 DAFCNJ010000000
VERSION     DAFCNJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.guided.109"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(40..1026)
                     /locus_tag="LNE35_003105"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="LNE35_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="LNE35_003106"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="LNE35_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK6912161.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="LNE35_003107"
     CDS             1851..3005
                     /locus_tag="LNE35_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK6912162.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="LNE35_003108"
     CDS             3220..4188
                     /locus_tag="LNE35_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK6912163.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="LNE35_003109"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="LNE35_003109"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK6912164.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="LNE35_003110"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="LNE35_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DAFCNJ010000240         1781 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.guided.155, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000240 DAFCNJ010000000
VERSION     DAFCNJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.guided.155"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..416
                     /locus_tag="LNE35_003111"
     CDS             24..416
                     /locus_tag="LNE35_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6912165.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="LNE35_003112"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="LNE35_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBK6912166.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DAFCNJ010000241         1504 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.guided.161, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000241 DAFCNJ010000000
VERSION     DAFCNJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.guided.161"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            262..345
                     /locus_tag="LNE35_003113"
                     /pseudo
     CDS             262..345
                     /locus_tag="LNE35_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="LNE35_003114"
     CDS             394..477
                     /locus_tag="LNE35_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBK6912167.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="LNE35_003115"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="LNE35_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBK6912168.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAFCNJ010000242         1382 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.guided.167, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000242 DAFCNJ010000000
VERSION     DAFCNJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.guided.167"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1311
                     /locus_tag="LNE35_003116"
     CDS             <1..1311
                     /locus_tag="LNE35_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HBK6912169.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAFCNJ010000243         1350 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.guided.168, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000243 DAFCNJ010000000
VERSION     DAFCNJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.guided.168"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            47..298
                     /locus_tag="LNE35_003117"
                     /pseudo
     CDS             47..298
                     /locus_tag="LNE35_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="LNE35_003118"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="LNE35_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBK6912170.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAFCNJ010000244         1194 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V3860
            SAMN22631203-rid13287963.guided.177, whole genome shotgun sequence.
ACCESSION   DAFCNJ010000244 DAFCNJ010000000
VERSION     DAFCNJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631203
            Sequence Read Archive: SRR16608668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171173.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 2,976
            CDSs (with protein)               :: 2,976
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 26 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 21 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631203-rid13287963.guided.177"
                     /isolate="V3860"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-01-26"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="LNE35_003119"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="LNE35_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBK6912171.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
